Cp4.1LG04g09960 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g09960
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionproline-rich receptor-like protein kinase PERK1
LocationCp4.1LG04: 9750604 .. 9753534 (-)
RNA-Seq ExpressionCp4.1LG04g09960
SyntenyCp4.1LG04g09960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTTTTAATTTTTTATATTTTTGTGTTGACGTCGACAGCTCACACATTGCAGATTATGTGCAATAGCAATTATACATCATGTATTGGTAATTTGTTTGGTATACTGCAAATTCCGCCATTGTTATCTTCTTCTACTTCCAGTTGCAGAGCAGGGATTTTCAATCTTCTTGAATAGAGTGTTTTTCCTTATATAACCAAGACCCATTTCATTGTTTTCGATTTCTGCTTCTCTCTGGGACTGAAATTTTGGGCGAAAATGTCGTCTCCTGCTCCCATCTCGCCGCCGGGGACTAACTCGACCTCACCCCCACCACCGACCACGGATGCACCACCTCCAGCATCTCCATCTCTCTCACCACCTCCTCCTGCTTCATCGCCGCCGCCTCCGAACAGTACCACTCCATCTCCGCCTACTTCTTCTCCTTCCCCGCCGGACGTGCCTTCGAGCTCTCCCCCGCCGCCGCCTACCGACTCGACAGCGCCACCGCAACTTCCGAGTCCGACGCCGCCATCCAATTCTAGTACACCTGTGAATCCTGGAGGGAATCAGGCTCCACCGTCGGAAACGGCGCCGTCTCCCTCAACATCGTCGGGAGTTTCGGCGGGACTTGTGTCTGGGGTTGCCGTTGGAGTGGTGGTGGTGGTTGTAGCGTTGATTGCTATGTGTTTTTATCTAAGAAAGAAGAGGAGACGCGACGAGGAGGCGTACTACCGGCCACCGCATCCGCCTGCGCCACCGGCGGCGTTTAAAGGTGAGAACCCTCAACCCACACAAATTTCTCAACTAATCCCTAAATTAATTGTCGAATTTGACATCAAGTTTTATGAAACAAATGAATTTCAGACGGCCCATACGGTCCCCAACAGCACCATTGGCAATCACATCCGCCGCCGCCAGGGGATCATATCGCGGGGGCAGTACCAAAGCCGTCTCCTCCGCCAGCATCACGGCCACCGCCGTCACCCCCGATTATAAACAGTAGCGGAGGGTCTGGATCTAATTATTCAGGGTCTGAAAATTCGTTGGCTCCAGCTCCATCTTCTGTTCCCTTGGGGTTCTCACAGAGCAGTTTCACTTACGAAGAATTAGCAATGGCGACAGATGGGTTTTCAGAAGCCAATCTCCTCGGACAAGGTGGGTTTGGTTATGTGCATAAAGGAGTTCTTCCTAACGGAAAGGAAGTGGCAGTGAAACAACTTAAAGCTGGAAGCGGACAAGGGGAGCGTGAATTCCAAGCAGAAGTTGAAATCATCAGCCGAGTTCATCATAGGCATCTTGTTTCTTTGGTCGGATATTGCATCACTGGCTCCCAAAGATTGCTGGTTTATGAATTCGTTCCCAACAACACATTGGAGTTTCACTTGCACGGTCTGTAATAAACCTCAATCCAATCCACCCTTTTGCTATCACTTCATGATCTTGACGCTCTTAACTTTCAGGGAAAGGACGACCTACCATGGATTGGCCAACCAGGCTCAAAATCGCTTTGGGATCTGCCAAGGGATTGGCCTACCTTCATGAGGACTGTAAGTATATATAGTGTTCGTGACCAGATCTCTTCTGGGTTTCTTCAATTTTCACGTTTTGAAGCCTTATCATTTACTTCTGATGCAATGCTCAGGTAATCCTAAGATCATTCATCGTGACATCAAAGCAGCAAACATACTTTTGGATCTCAAATTCGAAGCAAAGGTAATAATGGGAGCTTTCTAAATTCTTGAATTTGAATCTATAAAGAGACTCAAATGGGGTTGTTCCTGATATCTTTTTCCAGGTTGCAGATTTTGGGCTTGCCAAGTTGTCTTCTGATGTTAATACCCATGTTTCAACTCGAGTCATGGGAACTTTCGGGTATGTTAATACCCTTTGGAACCATTTCTTGACAAATTCATATTTCTGAACTCATGAATTTGATATCATGCTATGCCAGGTATCTTGCTCCTGAATACGCTTCAAGTGGGAAACTAACTGAGAAATCAGATGTGTTCTCCTATGGAGTAATGCTTCTGGAGTTGATTACTGGACGCAGACCTGTTGATCCAACTCAATCATTCATGGACGATGGATTGCTCGATTGGGTAAGCCCATTACCGAATTCTGTTCAAATTCAGCATCTCTGCAAACCCAATTCAGACGTTGAAAAACTAAATCAACCCATCTTGTCAATTTCAGGCAAGGCCATTACTACTTCGGGCAACAGAAAATGGAAACTACGACGGTTTGGTAGATCCAAAGCTGCGAGATAACCACGACCACAACGAGATGGCGCGCATGGTTGCTTGCGCTGCGGCCTGTGTGCGTCATTCGGCGAGGCGTCGACCACGGATGAGTCAAGTGGTTCATGCTCTTGAAGGAGAAGCCTCGCTTTCTGATCTGAACGAAGGAATCCGACCTGGACATAGCACTGTGTACAGTTCTTATGGAAGCTCCGACTACGACACGGCTCAGTACAACGAGGATCTGAAGAAGTTTAGGAAGATGGCGTTGGCGAGCCAGGAGTACGGCAGCGAGTACAGCGAGCCGACAAGCGAGTATGGGCTGTACCCATCTGGGTCGAGTGGCGATGGGCAAACAACGAGGGAAATGGAGACGAGAACGATGAAGAAAGAAAGTGGATGATTGATTTAGTGGAAGCTCGTGATGATGGTGAAGCAAAGATGCTTCATTTCTGTAATGTGTACAAACTACAACACTTTTTTCTGTGTTTGTTTTGGATTATAGGGATATTCTTTCATCTGTAGTGTGGTTCGTAAAATGGAAGAGCGTTCGGATCAAAGATTGGATTATATTTTGTCCACAGAAAACAAGTTATGAGATGGACTTCGATTCTTTTATTTGTTGTTTGGGCGATTGTATAGCCAATAGCAATTAGTAACCATATGGATCATTTAGTGCACTGATATTTGCAGCTCCAGTAGCTGGAATCCAC

mRNA sequence

ATTTTTTAATTTTTTATATTTTTGTGTTGACGTCGACAGCTCACACATTGCAGATTATGTGCAATAGCAATTATACATCATGTATTGGTAATTTGTTTGGTATACTGCAAATTCCGCCATTGTTATCTTCTTCTACTTCCAGTTGCAGAGCAGGGATTTTCAATCTTCTTGAATAGAGTGTTTTTCCTTATATAACCAAGACCCATTTCATTGTTTTCGATTTCTGCTTCTCTCTGGGACTGAAATTTTGGGCGAAAATGTCGTCTCCTGCTCCCATCTCGCCGCCGGGGACTAACTCGACCTCACCCCCACCACCGACCACGGATGCACCACCTCCAGCATCTCCATCTCTCTCACCACCTCCTCCTGCTTCATCGCCGCCGCCTCCGAACAGTACCACTCCATCTCCGCCTACTTCTTCTCCTTCCCCGCCGGACGTGCCTTCGAGCTCTCCCCCGCCGCCGCCTACCGACTCGACAGCGCCACCGCAACTTCCGAGTCCGACGCCGCCATCCAATTCTAGTACACCTGTGAATCCTGGAGGGAATCAGGCTCCACCGTCGGAAACGGCGCCGTCTCCCTCAACATCGTCGGGAGTTTCGGCGGGACTTGTGTCTGGGGTTGCCGTTGGAGTGGTGGTGGTGGTTGTAGCGTTGATTGCTATGTGTTTTTATCTAAGAAAGAAGAGGAGACGCGACGAGGAGGCGTACTACCGGCCACCGCATCCGCCTGCGCCACCGGCGGCGTTTAAAGACGGCCCATACGGTCCCCAACAGCACCATTGGCAATCACATCCGCCGCCGCCAGGGGATCATATCGCGGGGGCAGTACCAAAGCCGTCTCCTCCGCCAGCATCACGGCCACCGCCGTCACCCCCGATTATAAACAGTAGCGGAGGGTCTGGATCTAATTATTCAGGGTCTGAAAATTCGTTGGCTCCAGCTCCATCTTCTGTTCCCTTGGGGTTCTCACAGAGCAGTTTCACTTACGAAGAATTAGCAATGGCGACAGATGGGTTTTCAGAAGCCAATCTCCTCGGACAAGGTGGGTTTGGTTATGTGCATAAAGGAGTTCTTCCTAACGGAAAGGAAGTGGCAGTGAAACAACTTAAAGCTGGAAGCGGACAAGGGGAGCGTGAATTCCAAGCAGAAGTTGAAATCATCAGCCGAGTTCATCATAGGCATCTTGTTTCTTTGGTCGGATATTGCATCACTGGCTCCCAAAGATTGCTGGTTTATGAATTCGTTCCCAACAACACATTGGAGTTTCACTTGCACGGTAATCCTAAGATCATTCATCGTGACATCAAAGCAGCAAACATACTTTTGGATCTCAAATTCGAAGCAAAGGTTGCAGATTTTGGGCTTGCCAAGTTGTCTTCTGATGTTAATACCCATGTTTCAACTCGAGTCATGGGAACTTTCGGGTATCTTGCTCCTGAATACGCTTCAAGTGGGAAACTAACTGAGAAATCAGATGTGTTCTCCTATGGAGTAATGCTTCTGGAGTTGATTACTGGACGCAGACCTGTTGATCCAACTCAATCATTCATGGACGATGGATTGCTCGATTGGGCAAGGCCATTACTACTTCGGGCAACAGAAAATGGAAACTACGACGGTTTGGTAGATCCAAAGCTGCGAGATAACCACGACCACAACGAGATGGCGCGCATGGTTGCTTGCGCTGCGGCCTGTGTGCGTCATTCGGCGAGGCGTCGACCACGGATGAGTCAAGTGGTTCATGCTCTTGAAGGAGAAGCCTCGCTTTCTGATCTGAACGAAGGAATCCGACCTGGACATAGCACTGTGTACAGTTCTTATGGAAGCTCCGACTACGACACGGCTCAGTACAACGAGGATCTGAAGAAGTTTAGGAAGATGGCGTTGGCGAGCCAGGAGTACGGCAGCGAGTACAGCGAGCCGACAAGCGAGTATGGGCTGTACCCATCTGGGTCGAGTGGCGATGGGCAAACAACGAGGGAAATGGAGACGAGAACGATGAAGAAAGAAAGTGGATGATTGATTTAGTGGAAGCTCGTGATGATGGTGAAGCAAAGATGCTTCATTTCTGTAATGTGTACAAACTACAACACTTTTTTCTGTGTTTGTTTTGGATTATAGGGATATTCTTTCATCTGTAGTGTGGTTCGTAAAATGGAAGAGCGTTCGGATCAAAGATTGGATTATATTTTGTCCACAGAAAACAAGTTATGAGATGGACTTCGATTCTTTTATTTGTTGTTTGGGCGATTGTATAGCCAATAGCAATTAGTAACCATATGGATCATTTAGTGCACTGATATTTGCAGCTCCAGTAGCTGGAATCCAC

Coding sequence (CDS)

ATGTCGTCTCCTGCTCCCATCTCGCCGCCGGGGACTAACTCGACCTCACCCCCACCACCGACCACGGATGCACCACCTCCAGCATCTCCATCTCTCTCACCACCTCCTCCTGCTTCATCGCCGCCGCCTCCGAACAGTACCACTCCATCTCCGCCTACTTCTTCTCCTTCCCCGCCGGACGTGCCTTCGAGCTCTCCCCCGCCGCCGCCTACCGACTCGACAGCGCCACCGCAACTTCCGAGTCCGACGCCGCCATCCAATTCTAGTACACCTGTGAATCCTGGAGGGAATCAGGCTCCACCGTCGGAAACGGCGCCGTCTCCCTCAACATCGTCGGGAGTTTCGGCGGGACTTGTGTCTGGGGTTGCCGTTGGAGTGGTGGTGGTGGTTGTAGCGTTGATTGCTATGTGTTTTTATCTAAGAAAGAAGAGGAGACGCGACGAGGAGGCGTACTACCGGCCACCGCATCCGCCTGCGCCACCGGCGGCGTTTAAAGACGGCCCATACGGTCCCCAACAGCACCATTGGCAATCACATCCGCCGCCGCCAGGGGATCATATCGCGGGGGCAGTACCAAAGCCGTCTCCTCCGCCAGCATCACGGCCACCGCCGTCACCCCCGATTATAAACAGTAGCGGAGGGTCTGGATCTAATTATTCAGGGTCTGAAAATTCGTTGGCTCCAGCTCCATCTTCTGTTCCCTTGGGGTTCTCACAGAGCAGTTTCACTTACGAAGAATTAGCAATGGCGACAGATGGGTTTTCAGAAGCCAATCTCCTCGGACAAGGTGGGTTTGGTTATGTGCATAAAGGAGTTCTTCCTAACGGAAAGGAAGTGGCAGTGAAACAACTTAAAGCTGGAAGCGGACAAGGGGAGCGTGAATTCCAAGCAGAAGTTGAAATCATCAGCCGAGTTCATCATAGGCATCTTGTTTCTTTGGTCGGATATTGCATCACTGGCTCCCAAAGATTGCTGGTTTATGAATTCGTTCCCAACAACACATTGGAGTTTCACTTGCACGGTAATCCTAAGATCATTCATCGTGACATCAAAGCAGCAAACATACTTTTGGATCTCAAATTCGAAGCAAAGGTTGCAGATTTTGGGCTTGCCAAGTTGTCTTCTGATGTTAATACCCATGTTTCAACTCGAGTCATGGGAACTTTCGGGTATCTTGCTCCTGAATACGCTTCAAGTGGGAAACTAACTGAGAAATCAGATGTGTTCTCCTATGGAGTAATGCTTCTGGAGTTGATTACTGGACGCAGACCTGTTGATCCAACTCAATCATTCATGGACGATGGATTGCTCGATTGGGCAAGGCCATTACTACTTCGGGCAACAGAAAATGGAAACTACGACGGTTTGGTAGATCCAAAGCTGCGAGATAACCACGACCACAACGAGATGGCGCGCATGGTTGCTTGCGCTGCGGCCTGTGTGCGTCATTCGGCGAGGCGTCGACCACGGATGAGTCAAGTGGTTCATGCTCTTGAAGGAGAAGCCTCGCTTTCTGATCTGAACGAAGGAATCCGACCTGGACATAGCACTGTGTACAGTTCTTATGGAAGCTCCGACTACGACACGGCTCAGTACAACGAGGATCTGAAGAAGTTTAGGAAGATGGCGTTGGCGAGCCAGGAGTACGGCAGCGAGTACAGCGAGCCGACAAGCGAGTATGGGCTGTACCCATCTGGGTCGAGTGGCGATGGGCAAACAACGAGGGAAATGGAGACGAGAACGATGAAGAAAGAAAGTGGATGA

Protein sequence

MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPDVPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVSGVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHPPPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDGQTTREMETRTMKKESG
Homology
BLAST of Cp4.1LG04g09960 vs. ExPASy Swiss-Prot
Match: Q9LV48 (Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 GN=PERK1 PE=1 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 3.2e-184
Identity = 389/640 (60.78%), Postives = 456/640 (71.25%), Query Frame = 0

Query: 3   SPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPDVP 62
           SP+P SPP  ++T+ PPP   +PPP +       P+S PP P++ + SPP SSP PP +P
Sbjct: 12  SPSPPSPPTNSTTTTPPPAASSPPPTT------TPSSPPPSPSTNSTSPPPSSPLPPSLP 71

Query: 63  SSSPP---PPPTDSTAP-----PQLPSPTPPSNSSTPVNP---------GGNQAPPSETA 122
             SPP    PP    +P     P  PSPT PSN  +P +P         G     PS T 
Sbjct: 72  PPSPPGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSGSTPRTPSNTK 131

Query: 123 PSP--STSSGVSAGLVSGVAVGVVVVVVALIAMCFYLRKKRRR---DEEAYYRPPHPPAP 182
           PSP   +S G+S G+V G+A+G V ++V L  +C   +KKRRR   DE AYY PP PP+ 
Sbjct: 132 PSPPSDSSDGLSTGVVVGIAIGGVAILVILTLICLLCKKKRRRRHDDEAAYYVPPPPPSG 191

Query: 183 PAAFKDGPYGPQQHHWQSH--PPPPGDHIAGAVPKPSP--PPASRPPPSPPIINSSGGSG 242
           P A   GPYG QQ +WQ      P  +H+  ++P P P  PP   PPP PP    S   G
Sbjct: 192 PKA--GGPYGGQQQYWQQQNASRPSDNHVVTSLPPPKPPSPPRKPPPPPPPPAFMSSSGG 251

Query: 243 SNYSGSENSLAPAPSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNG 302
           S+YS       P+P  V LGFS+S+FTYEEL+ AT+GFSEANLLGQGGFGYVHKG+LP+G
Sbjct: 252 SDYSDLPVLPPPSPGLV-LGFSKSTFTYEELSRATNGFSEANLLGQGGFGYVHKGILPSG 311

Query: 303 KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLE 362
           KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSL+GYC+ G QRLLVYEFVPNN LE
Sbjct: 312 KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLE 371

Query: 363 FHLHG-----------------------------NPKIIHRDIKAANILLDLKFEAKVAD 422
           FHLHG                             NPKIIHRDIKA+NIL+D KFEAKVAD
Sbjct: 372 FHLHGKGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVAD 431

Query: 423 FGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDP 482
           FGLAK++SD NTHVSTRVMGTFGYLAPEYA+SGKLTEKSDVFS+GV+LLELITGRRPVD 
Sbjct: 432 FGLAKIASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDA 491

Query: 483 TQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARR 542
              ++DD L+DWARPLL RA+E G+++GL D K+ + +D  EMARMVACAAACVRHSARR
Sbjct: 492 NNVYVDDSLVDWARPLLNRASEEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARR 551

Query: 543 RPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSY-GSSDYDTAQYNEDLKKFRKMALAS 585
           RPRMSQ+V ALEG  SLSDLNEG+RPGHS VYSSY GS+DYDT+QYN+D+ KFRKMAL +
Sbjct: 552 RPRMSQIVRALEGNVSLSDLNEGMRPGHSNVYSSYGGSTDYDTSQYNDDMIKFRKMALGT 611

BLAST of Cp4.1LG04g09960 vs. ExPASy Swiss-Prot
Match: Q9LK03 (Proline-rich receptor-like protein kinase PERK2 OS=Arabidopsis thaliana OX=3702 GN=PERK2 PE=2 SV=3)

HSP 1 Score: 542.7 bits (1397), Expect = 5.0e-153
Identity = 359/628 (57.17%), Postives = 422/628 (67.20%), Query Frame = 0

Query: 3   SPAPI---SPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTP-SPPTSSPSP 62
           SP P    SPP T S  PP PTT +PPP SPS+  PP   SPPP +   P SPP  SP+ 
Sbjct: 108 SPPPAITPSPPLTPSPLPPSPTTPSPPPPSPSIPSPPLTPSPPPSSPLRPSSPPPPSPAT 167

Query: 63  PDVPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAP--PSET---APSPSTSSG 122
           P  P  SPPPP T  T PP++ S +PP     P +P G ++P  PS T   +P   +S  
Sbjct: 168 PSTPPRSPPPPST-PTPPPRVGSLSPP----PPASPSGGRSPSTPSTTPGSSPPAQSSKE 227

Query: 123 VSAGLVSGVAVGVVVVVVALIAMCFYL-RKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQ 182
           +S G + G+A+G   V++  +A+ F+L +KKRRRD E         APPA     PYG Q
Sbjct: 228 LSKGAMVGIAIGGGFVLLVALALIFFLCKKKRRRDNE---------APPAPIDGVPYGGQ 287

Query: 183 QHHWQSHPPPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSG---SNYSGSENSLAPA 242
           Q   Q +     DH+  +VP P  P  S  PP PP   SSG SG   SNY  S+ S+ P 
Sbjct: 288 Q---QQNASRRSDHVVMSVPPPKSP--SSAPPRPPHFMSSGSSGDYDSNY--SDQSVLPP 347

Query: 243 PS---SVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKA 302
           PS   ++ LG  Q +F YEEL+ AT+GFSEANLLGQGGFGYV KG+L NGKEVAVKQLK 
Sbjct: 348 PSPGLALGLGIYQGTFNYEELSRATNGFSEANLLGQGGFGYVFKGMLRNGKEVAVKQLKE 407

Query: 303 GSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG----- 362
           GS QGEREFQAEV IISRVHHRHLV+LVGYCI  +QRLLVYEFVPNNTLEFHLHG     
Sbjct: 408 GSSQGEREFQAEVGIISRVHHRHLVALVGYCIADAQRLLVYEFVPNNTLEFHLHGKGRPT 467

Query: 363 ------------------------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDV 422
                                   NPKIIHRDIKA+NIL+D KFEAKVADFGLAK++SD 
Sbjct: 468 MEWSSRLKIAVGSAKGLSYLHENCNPKIIHRDIKASNILIDFKFEAKVADFGLAKIASDT 527

Query: 423 NTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLL 482
           NTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS+GV+LLELITGRRP+D      D+ L+
Sbjct: 528 NTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVVLLELITGRRPIDVNNVHADNSLV 587

Query: 483 DWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHA 542
           DWARPLL + +E GN++ +VD KL + +D  EMARMVACAAACVR +A RRPRM QV   
Sbjct: 588 DWARPLLNQVSELGNFEVVVDKKLNNEYDKEEMARMVACAAACVRSTAPRRPRMDQVARV 647

Query: 543 LEGEASLSDLNEGIRPGHSTVY-SSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEP 585
           LEG  S SDLN+GI PGHS VY SS GS+DYD++Q NE + KFRK+ L +Q+    YS P
Sbjct: 648 LEGNISPSDLNQGITPGHSNVYGSSGGSTDYDSSQDNEGMNKFRKVGLETQDL---YSNP 707

BLAST of Cp4.1LG04g09960 vs. ExPASy Swiss-Prot
Match: Q9XI96 (Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 GN=PERK7 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.8e-134
Identity = 343/668 (51.35%), Postives = 413/668 (61.83%), Query Frame = 0

Query: 4   PAPISPPGTN---STSPPP----PTTDAPPPASPSLSPPP--PASSPPP--PNSTTPSPP 63
           P+P SPP +N   +TSPPP     TT  PPP+SP ++PPP     SPPP  P +++    
Sbjct: 17  PSPPSPPSSNDQQTTSPPPSDNQETTSPPPPSSPDIAPPPQQQQESPPPPLPENSSDGSS 76

Query: 64  TSSPSPPDVPSSSPPPPPTDSTAPPQLP----------------------------SPTP 123
           +SSP PP   SS    PP  ST+PPQ                              S TP
Sbjct: 77  SSSPPPPSDSSSQSQSPPPPSTSPPQQSDNNGNKGNNNENNKGNDGSSGDGGNKNMSHTP 136

Query: 124 PSNSSTPVNPGGNQ----APPSETAPSPSTSS-GVSAGLVSGV--AVGVVVVVVALIAMC 183
           P  S T  +   +Q    APP+  + S S+S+ G++ G V G+  A G++ +V+ L+ +C
Sbjct: 137 PPPSKTSDHSSHSQPRSLAPPTSNSGSNSSSNDGLNIGAVIGLVAAAGILFIVMILLCVC 196

Query: 184 FYLRKKRRR--DEEAYYRPPHPPAPPAA----FKDGPYGPQQHHWQSH------PPPPGD 243
            + +KK++   D+  YY     PA        +       QQ H+  +      PPPPG 
Sbjct: 197 CFRKKKKKSKLDQMPYYGSNAYPAGKTGGDQYYNQNAATQQQQHYNQNDHIVNLPPPPGS 256

Query: 244 HIAGAVPKPSPPPAS--RPPPSPPI-------INSSGGSGSNYSGS--ENSLAPAPSSVP 303
                V  P PPP    +P PSPP        +  SG   SN+S      SL P   SV 
Sbjct: 257 MGTNWVSSPPPPPPGNWQPMPSPPAPVSGGANVIQSGEMSSNFSSGPYAPSLPPPHPSVA 316

Query: 304 LGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGERE 363
           LGF+ S+FTYEELA AT GFS+  LLGQGGFGYVHKG+LPNGKE+AVK LKAGSGQGERE
Sbjct: 317 LGFNNSTFTYEELASATQGFSKDRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGERE 376

Query: 364 FQAEVEIISRVHHRHLVSLVGYCI-TGSQRLLVYEFVPNNTLEFHLHG------------ 423
           FQAEVEIISRVHHRHLVSLVGYC   G QRLLVYEF+PN+TLEFHLHG            
Sbjct: 377 FQAEVEIISRVHHRHLVSLVGYCSNAGGQRLLVYEFLPNDTLEFHLHGKSGTVMDWPTRL 436

Query: 424 -----------------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTR 483
                            +PKIIHRDIKA+NILLD  FEAKVADFGLAKLS D NTHVSTR
Sbjct: 437 KIALGSAKGLAYLHEDCHPKIIHRDIKASNILLDHNFEAKVADFGLAKLSQDNNTHVSTR 496

Query: 484 VMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLL 543
           VMGTFGYLAPEYASSGKLTEKSDVFS+GVMLLELITGR PVD     M+D L+DWARPL 
Sbjct: 497 VMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGRGPVD-LSGDMEDSLVDWARPLC 556

Query: 544 LRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASL 571
           +R  ++G Y  LVDP L   ++  EMARMVACAAA VRHS RRRP+MSQ+V  LEG+ASL
Sbjct: 557 MRVAQDGEYGELVDPFLEHQYEPYEMARMVACAAAAVRHSGRRRPKMSQIVRTLEGDASL 616

BLAST of Cp4.1LG04g09960 vs. ExPASy Swiss-Prot
Match: Q9ZNQ8 (Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 GN=PERK4 PE=1 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.2e-133
Identity = 326/630 (51.75%), Postives = 399/630 (63.33%), Query Frame = 0

Query: 5   APISPPGTNSTSPPPP------TTDAPPPASPSLSPPPP---ASSPPPPNSTTPSPPTSS 64
           +P S P TNSTS P P      TT +PP  SP  SP PP   +SS PPP+ST+P P   +
Sbjct: 4   SPESAPPTNSTSSPSPPSNTNSTTSSPPAPSPP-SPTPPQGDSSSSPPPDSTSP-PAPQA 63

Query: 65  PSPPDVPSSSPPPPPT-------------DSTAPPQLPSPTPPSNSSTPVNPGGNQAPPS 124
           P+PP+  ++SP PP                +  PP   SP  P + S   N G   +PP 
Sbjct: 64  PNPPNSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPPSRSNGDNGGSRSSPPG 123

Query: 125 ETAPS----PSTSSGVSAG-----------LVSGVAVGVVVVVVALIAMCFYLRKKRRRD 184
           +T  S    P +S G S G           ++ GV VG  ++++ LI +C  LR+K++R 
Sbjct: 124 DTGGSRSDNPPSSGGSSGGGGGGRSNTNTAIIVGVLVGAGLLMIVLIIVC--LRRKKKRK 183

Query: 185 EEAYYRPPHPPAPPAAFKDGPYGPQQH--------HWQSHPPPPGDHIAGAVPKPSPPPA 244
           +  Y      P P    +   YG   +        +W  +         G      P   
Sbjct: 184 DSFY------PEPMKGNQYQYYGNNNNNNASQNYPNWHLNSQGQNQQSTGGWGGGGP--- 243

Query: 245 SRPPPSPPIINSSGGSGSNYSGSENSLAPAPS-SVPLGFSQSSFTYEELAMATDGFSEAN 304
              PP PP + +SG   S YSG    + P PS ++ LGF++S+FTY+ELA AT GF++AN
Sbjct: 244 --SPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTYQELAAATGGFTDAN 303

Query: 305 LLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCI 364
           LLGQGGFGYVHKGVLP+GKEVAVK LKAGSGQGEREFQAEV+IISRVHHR+LVSLVGYCI
Sbjct: 304 LLGQGGFGYVHKGVLPSGKEVAVKSLKAGSGQGEREFQAEVDIISRVHHRYLVSLVGYCI 363

Query: 365 TGSQRLLVYEFVPNNTLEFHLHG-----------------------------NPKIIHRD 424
              QR+LVYEFVPN TLE+HLHG                             +P+IIHRD
Sbjct: 364 ADGQRMLVYEFVPNKTLEYHLHGKNLPVMEFSTRLRIALGAAKGLAYLHEDCHPRIIHRD 423

Query: 425 IKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF 484
           IK+ANILLD  F+A VADFGLAKL+SD NTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF
Sbjct: 424 IKSANILLDFNFDAMVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF 483

Query: 485 SYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNE 544
           SYGVMLLELITG+RPVD + + MDD L+DWARPL+ RA E+GN++ L D +L  N++  E
Sbjct: 484 SYGVMLLELITGKRPVDNSIT-MDDTLVDWARPLMARALEDGNFNELADARLEGNYNPQE 543

Query: 545 MARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSYG-SSDYD 559
           MARMV CAAA +RHS R+RP+MSQ+V ALEGE SL  LNEG++PGHS VY S G SSDY 
Sbjct: 544 MARMVTCAAASIRHSGRKRPKMSQIVRALEGEVSLDALNEGVKPGHSNVYGSLGASSDYS 603

BLAST of Cp4.1LG04g09960 vs. ExPASy Swiss-Prot
Match: Q8GX23 (Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 GN=PERK5 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 6.0e-130
Identity = 324/664 (48.80%), Postives = 408/664 (61.45%), Query Frame = 0

Query: 2   SSPAPISPPGTNSTSPPPPTTDAPPP-ASPSLSPP--PPASSPPPPNSTTPSPPTSSPSP 61
           +SP+  S P T  +SPPP +  APPP  S S SPP  PP     PP S + SPP  +   
Sbjct: 24  TSPSNESSPPTPPSSPPPSSISAPPPDISASFSPPPAPPTQETSPPTSPSSSPPVVANPS 83

Query: 62  PDVPSSSPPPPPTDST-----APPQLPS------PTPPS-----------------NSST 121
           P  P +  PP P  ST     APPQ PS      PTPPS                 + ST
Sbjct: 84  PQTPENPSPPAPEGSTPVTPPAPPQTPSNQSPERPTPPSPGANDDRNRTNGGNNNRDGST 143

Query: 122 PVNP---------GGNQAPPSETAP-----SPSTSSG----VSAGLVSGVAVGVVVVVVA 181
           P  P         GG+ +PP   +P        +SSG     + GL+ GV VG  ++++ 
Sbjct: 144 PSPPSSGNRTSGDGGSPSPPRSISPPQNSGDSDSSSGNHPQANIGLIIGVLVGAGLLLLL 203

Query: 182 LIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPY-GPQQHHWQSHPPPPGDHIAGAV 241
            + +C    +K+++         +       + + PY G    +   +   P DH+    
Sbjct: 204 AVCICICCNRKKKKKSPQVNHMHY-------YNNNPYGGAPSGNGGYYKGTPQDHVVNMA 263

Query: 242 PKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQSSFTYEELAMAT 301
            +       + P S P  ++S  +G        +  P+P +  LG +QS+FTY+EL++AT
Sbjct: 264 GQGGGNWGPQQPVSGPHSDASNLTG-------RTAIPSPQAATLGHNQSTFTYDELSIAT 323

Query: 302 DGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLV 361
           +GF+++NLLGQGGFGYVHKGVLP+GKEVAVK LK GSGQGEREFQAEV+IISRVHHRHLV
Sbjct: 324 EGFAQSNLLGQGGFGYVHKGVLPSGKEVAVKSLKLGSGQGEREFQAEVDIISRVHHRHLV 383

Query: 362 SLVGYCITGSQRLLVYEFVPNNTLEFHLHG-----------------------------N 421
           SLVGYCI+G QRLLVYEF+PNNTLEFHLHG                             +
Sbjct: 384 SLVGYCISGGQRLLVYEFIPNNTLEFHLHGKGRPVLDWPTRVKIALGSARGLAYLHEDCH 443

Query: 422 PKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKL 481
           P+IIHRDIKAANILLD  FE KVADFGLAKLS D  THVSTRVMGTFGYLAPEYASSGKL
Sbjct: 444 PRIIHRDIKAANILLDFSFETKVADFGLAKLSQDNYTHVSTRVMGTFGYLAPEYASSGKL 503

Query: 482 TEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLR 541
           ++KSDVFS+GVMLLELITGR P+D T   M+D L+DWARPL L+A ++G+Y+ L DP+L 
Sbjct: 504 SDKSDVFSFGVMLLELITGRPPLDLTGE-MEDSLVDWARPLCLKAAQDGDYNQLADPRLE 563

Query: 542 DNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSY 585
            N+ H EM +M +CAAA +RHSARRRP+MSQ+V ALEG+ S+ DL+EG RPG ST Y S 
Sbjct: 564 LNYSHQEMVQMASCAAAAIRHSARRRPKMSQIVRALEGDMSMDDLSEGTRPGQST-YLSP 623

BLAST of Cp4.1LG04g09960 vs. NCBI nr
Match: XP_023529433.1 (proline-rich receptor-like protein kinase PERK1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1091 bits (2822), Expect = 0.0
Identity = 587/616 (95.29%), Postives = 587/616 (95.29%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD
Sbjct: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS
Sbjct: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP
Sbjct: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. NCBI nr
Match: KAG6587962.1 (Proline-rich receptor-like protein kinase PERK1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1084 bits (2803), Expect = 0.0
Identity = 583/616 (94.64%), Postives = 584/616 (94.81%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD
Sbjct: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSS PPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS
Sbjct: 61  VPSSSSPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GV VGV+VVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP
Sbjct: 121 GVVVGVLVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAG VPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGTVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. NCBI nr
Match: XP_022933488.1 (proline-rich receptor-like protein kinase PERK1 [Cucurbita moschata])

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 581/616 (94.32%), Postives = 583/616 (94.64%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD
Sbjct: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPS+SSGVSAGLVS
Sbjct: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSSSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GV VGV+VVVVALIAM FYLRKKRRRDEEAYYRPPHPPAPPAAFKDG YGPQQHHWQSHP
Sbjct: 121 GVVVGVLVVVVALIAMWFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGQYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAG VPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGTVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. NCBI nr
Match: XP_023007117.1 (proline-rich receptor-like protein kinase PERK1 [Cucurbita maxima])

HSP 1 Score: 1066 bits (2758), Expect = 0.0
Identity = 575/616 (93.34%), Postives = 577/616 (93.67%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAP SPPGTNSTSPPPPT DAPPP+ PSLSPPPPASSPPPPNSTTPSPPTSSPSPP 
Sbjct: 1   MSSPAPTSPPGTNSTSPPPPTADAPPPSPPSLSPPPPASSPPPPNSTTPSPPTSSPSPPV 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSSPPPPPT+STAPPQLPSPTPPSNS TP NPGG QAPPSETAPSPSTSSGVSAGLVS
Sbjct: 61  VPSSSPPPPPTNSTAPPQLPSPTPPSNSDTPANPGGGQAPPSETAPSPSTSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GV VGVVVVVVALIAMCFYLRKKRRRDEEAYYRPP PPAPPAAFKDGPYGPQQHHWQSHP
Sbjct: 121 GVVVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPPPPAPPAAFKDGPYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAG VPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGTVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. NCBI nr
Match: XP_038879967.1 (proline-rich receptor-like protein kinase PERK1 [Benincasa hispida])

HSP 1 Score: 949 bits (2453), Expect = 0.0
Identity = 530/634 (83.60%), Postives = 550/634 (86.75%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPAS----------------SPPPP 60
           MSSPAP SPP TN+TSPPPPTT APPPA PSLSPPPP +                SPPPP
Sbjct: 1   MSSPAPTSPPATNTTSPPPPTTGAPPPAPPSLSPPPPDAASQPPPASSPPPTTTPSPPPP 60

Query: 61  NSTTPSPPTSSPSPPDVPSSSPPPPP-TDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSE 120
           +++ PSPPTSSPSPP  PSSS PPPP T+STAPPQLPSPTPPS S TP +PGGN +P   
Sbjct: 61  DTSAPSPPTSSPSPPTTPSSSSPPPPSTNSTAPPQLPSPTPPSGSGTPASPGGNLSP--- 120

Query: 121 TAPSPSTSSGVSAGLVSGVAV-GVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPA 180
             PSP +SSG SAGLV+GVA+ GVVVVVVALI M   LRKKRRRDEEAYYRPP    PP 
Sbjct: 121 --PSPPSSSGASAGLVAGVAIAGVVVVVVALIVMFLCLRKKRRRDEEAYYRPP----PPP 180

Query: 181 AFKDGPYGPQQHHWQSHPPPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGS 240
           AFKDGPYGPQQHHWQSH PPP DH+ GAVPKPSPPP+SRPP SPPIINSSGGSGSNYSGS
Sbjct: 181 AFKDGPYGPQQHHWQSHQPPPADHVVGAVPKPSPPPSSRPPLSPPIINSSGGSGSNYSGS 240

Query: 241 ENSLAPAPSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVK 300
           ENSLAPAPSS+PLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVK
Sbjct: 241 ENSLAPAPSSIPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVK 300

Query: 301 QLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG- 360
           QLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGS+RLLVYEFVPNNTLEFHLHG 
Sbjct: 301 QLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSKRLLVYEFVPNNTLEFHLHGK 360

Query: 361 ----------------------------NPKIIHRDIKAANILLDLKFEAKVADFGLAKL 420
                                       NPKIIHRDIKAANILLDLKFEAKVADFGLAKL
Sbjct: 361 GRPTMDWPTRLKIALGSAKGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKL 420

Query: 421 SSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMD 480
           SSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS+GVMLLE+ITGRRPVD TQSFMD
Sbjct: 421 SSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEMITGRRPVDTTQSFMD 480

Query: 481 DGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQ 540
           DGLLDWARPLLLRATE+G YDGLVDPKLRDN+DHNEMARMVACAAACVRHSARRRPRMSQ
Sbjct: 481 DGLLDWARPLLLRATEDGVYDGLVDPKLRDNYDHNEMARMVACAAACVRHSARRRPRMSQ 540

Query: 541 VVHALEGEASLSDLNEGIRPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEY 587
           VVHALEGEASLSDLNEGIRPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEY
Sbjct: 541 VVHALEGEASLSDLNEGIRPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEY 600

BLAST of Cp4.1LG04g09960 vs. ExPASy TrEMBL
Match: A0A6J1F4W3 (proline-rich receptor-like protein kinase PERK1 OS=Cucurbita moschata OX=3662 GN=LOC111440893 PE=4 SV=1)

HSP 1 Score: 1078 bits (2788), Expect = 0.0
Identity = 581/616 (94.32%), Postives = 583/616 (94.64%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD
Sbjct: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPS+SSGVSAGLVS
Sbjct: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSSSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GV VGV+VVVVALIAM FYLRKKRRRDEEAYYRPPHPPAPPAAFKDG YGPQQHHWQSHP
Sbjct: 121 GVVVGVLVVVVALIAMWFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGQYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAG VPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGTVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. ExPASy TrEMBL
Match: A0A6J1KXQ4 (proline-rich receptor-like protein kinase PERK1 OS=Cucurbita maxima OX=3661 GN=LOC111499712 PE=4 SV=1)

HSP 1 Score: 1066 bits (2758), Expect = 0.0
Identity = 575/616 (93.34%), Postives = 577/616 (93.67%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPD 60
           MSSPAP SPPGTNSTSPPPPT DAPPP+ PSLSPPPPASSPPPPNSTTPSPPTSSPSPP 
Sbjct: 1   MSSPAPTSPPGTNSTSPPPPTADAPPPSPPSLSPPPPASSPPPPNSTTPSPPTSSPSPPV 60

Query: 61  VPSSSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSAGLVS 120
           VPSSSPPPPPT+STAPPQLPSPTPPSNS TP NPGG QAPPSETAPSPSTSSGVSAGLVS
Sbjct: 61  VPSSSPPPPPTNSTAPPQLPSPTPPSNSDTPANPGGGQAPPSETAPSPSTSSGVSAGLVS 120

Query: 121 GVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHP 180
           GV VGVVVVVVALIAMCFYLRKKRRRDEEAYYRPP PPAPPAAFKDGPYGPQQHHWQSHP
Sbjct: 121 GVVVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPPPPAPPAAFKDGPYGPQQHHWQSHP 180

Query: 181 PPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240
           PPPGDHIAG VPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS
Sbjct: 181 PPPGDHIAGTVPKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQS 240

Query: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300
           SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE
Sbjct: 241 SFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVE 300

Query: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG------------------- 360
           IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                   
Sbjct: 301 IISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSA 360

Query: 361 ----------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420
                     NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY
Sbjct: 361 KGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGY 420

Query: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480
           LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG
Sbjct: 421 LAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENG 480

Query: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540
           NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI
Sbjct: 481 NYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGI 540

Query: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 587
           RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG
Sbjct: 541 RPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSEYSEPTSEYGLYPSGSSGDG 600

BLAST of Cp4.1LG04g09960 vs. ExPASy TrEMBL
Match: A0A1S3CJ37 (proline-rich receptor-like protein kinase PERK1 OS=Cucumis melo OX=3656 GN=LOC103501562 PE=4 SV=1)

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 524/636 (82.39%), Postives = 546/636 (85.85%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPAS-----------------SPPP 60
           MSSP+P SPP TN+TSPPPPTT APPPA+PSLSPPPP S                 SPPP
Sbjct: 1   MSSPSPTSPPATNTTSPPPPTTTAPPPATPSLSPPPPDSASQPPPASSSPPPSTTPSPPP 60

Query: 61  PNSTTPSPPTSSPSPPDVPS-SSPPPPPTDSTAPPQLPSPTPPSNSSTPVNPGGNQAPPS 120
           P+++TPSPPTSSPSPP  PS SSP PP T+ST PPQLPSPTPPS S TP +PG N  P  
Sbjct: 61  PDTSTPSPPTSSPSPPTPPSTSSPAPPSTNSTTPPQLPSPTPPSGSGTPASPGENLTP-- 120

Query: 121 ETAPSPSTSSGVSAGLVSGVAV-GVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPP 180
               SPS SSGVSAGLV+GVA+ GVVVVVVALI M   +RKKRRRDEEAYYRPP    PP
Sbjct: 121 ---SSPSPSSGVSAGLVAGVAIAGVVVVVVALIVMFLCMRKKRRRDEEAYYRPP---PPP 180

Query: 181 AAFKDGPYGPQQHHWQSHPPPPGDHIAGAVPKPSPPPASRPPPSPPIINSSGGSGSNYSG 240
            A+KDGPYG QQHHWQSHPPPP DHI GAVPKPSPPPA RPP SPPIINSSGGSGSNYSG
Sbjct: 181 PAYKDGPYGQQQHHWQSHPPPPADHIVGAVPKPSPPPAPRPPLSPPIINSSGGSGSNYSG 240

Query: 241 SENSLAPAPSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAV 300
           SENSLAPA SS+PLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAV
Sbjct: 241 SENSLAPASSSIPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAV 300

Query: 301 KQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG 360
           KQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGS+RLLVYEFVPNNTLEFHLHG
Sbjct: 301 KQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSKRLLVYEFVPNNTLEFHLHG 360

Query: 361 -----------------------------NPKIIHRDIKAANILLDLKFEAKVADFGLAK 420
                                        NPKIIHRDIKAANILLDLKFEAKVADFGLAK
Sbjct: 361 KGRPTMDWPTRLKIALGSAKGLAYLHEDCNPKIIHRDIKAANILLDLKFEAKVADFGLAK 420

Query: 421 LSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFM 480
           LSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFS+GVMLLE+ITGRRPVD TQSFM
Sbjct: 421 LSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLEMITGRRPVDTTQSFM 480

Query: 481 DDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMS 540
           DDGLLDWARPLLLRATE+G+YDGLVDPKLRDN+DHNEMARMVACAAACVRHSARRRPRMS
Sbjct: 481 DDGLLDWARPLLLRATEDGHYDGLVDPKLRDNYDHNEMARMVACAAACVRHSARRRPRMS 540

Query: 541 QVVHALEGEASLSDLNEGIRPGHSTVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSE 587
           QVVHALEGE+SLSDLNEGIRPGHS+VYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSE
Sbjct: 541 QVVHALEGESSLSDLNEGIRPGHSSVYSSYGSSDYDTAQYNEDLKKFRKMALASQEYGSE 600

BLAST of Cp4.1LG04g09960 vs. ExPASy TrEMBL
Match: A0A6J1JJF0 (proline-rich receptor-like protein kinase PERK1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485132 PE=4 SV=1)

HSP 1 Score: 868 bits (2243), Expect = 1.70e-311
Identity = 506/691 (73.23%), Postives = 537/691 (77.71%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPAS--------------------- 60
           MSSP+P SPP  ++TSP PPTTDAPPP +PSLSPPPP +                     
Sbjct: 1   MSSPSPTSPPAPSTTSPLPPTTDAPPPEAPSLSPPPPDAASQPPPATSLPPSTTPSPPPP 60

Query: 61  ---------------SPPPPNSTTPSPPTSSPSPPDVPSSSPPPPPTDSTA------PPQ 120
                          SPPPP+STTPSPPT+SP PP  PSSS PPPP  +T       PP 
Sbjct: 61  DSATPSPPPPDSATPSPPPPDSTTPSPPTTSPLPPTAPSSSSPPPPDSATPSPPTSLPPT 120

Query: 121 LPS---PTPPSNSSTPVNPGGNQAPPS-----------ETAPSPSTSSGVSAGLVSGVAV 180
           LPS   P PPSNSSTP +PGG+ APPS           E  PS S SSG SAGLV+GV +
Sbjct: 121 LPSGSSPPPPSNSSTPTSPGGHDAPPSHSGTPTNPGGNEAPPSFSPSSGASAGLVTGVVI 180

Query: 181 -GVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHPPPP 240
            GVVVVVVA I +   LRKKRRRDEE+YYRPP    PP AFKDGPYG QQHHWQSHPPPP
Sbjct: 181 AGVVVVVVAXILLVLCLRKKRRRDEESYYRPP----PPGAFKDGPYGLQQHHWQSHPPPP 240

Query: 241 GDHIAGAVPKPSPPPASRPPPSPP----------IINSSGGSGSNYSGSENSLAP----- 300
            +H+ G VPKPSPPPASRPPPSP           II SSGGSGS YSGSE S AP     
Sbjct: 241 TNHVVGTVPKPSPPPASRPPPSPSPASRPPPSPAIIYSSGGSGSYYSGSEYSHAPVPAPA 300

Query: 301 ---APSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLK 360
              APSS+PLG+SQSSFTYEELAMATD FSEANLLGQGGFGYVHKGVLPNGKEVAVKQLK
Sbjct: 301 PAPAPSSIPLGYSQSSFTYEELAMATDRFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLK 360

Query: 361 AGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG---- 420
           AGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHG    
Sbjct: 361 AGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRP 420

Query: 421 -------------------------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSD 480
                                    NPKIIHRDIKAANILLDLKFEA VADFGLAKLSSD
Sbjct: 421 TMDWPTRLKIALGSAKGLAYLHEDCNPKIIHRDIKAANILLDLKFEAMVADFGLAKLSSD 480

Query: 481 VNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGL 540
           VNTHVSTRVMGTFGYLAPEYASSGKLT+KSDVFS+GVMLLE+ITGRRPVD TQS+MDDGL
Sbjct: 481 VNTHVSTRVMGTFGYLAPEYASSGKLTDKSDVFSFGVMLLEMITGRRPVDTTQSYMDDGL 540

Query: 541 LDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVH 587
           LDWARPLLLRA E+GNYDGLVDPKLRDN+DHNEM RM+ACAAACVRHSARRRPRMSQVVH
Sbjct: 541 LDWARPLLLRAAEDGNYDGLVDPKLRDNYDHNEMGRMIACAAACVRHSARRRPRMSQVVH 600

BLAST of Cp4.1LG04g09960 vs. ExPASy TrEMBL
Match: A0A6J1JHF7 (proline-rich receptor-like protein kinase PERK1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485132 PE=4 SV=1)

HSP 1 Score: 865 bits (2235), Expect = 1.13e-310
Identity = 505/666 (75.83%), Postives = 534/666 (80.18%), Query Frame = 0

Query: 1   MSSPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPP--ASSPPP----PNSTTPSPP-- 60
           MSSP+P SPP  ++TSP PPTTDAPPP +PSLSPPPP  AS PPP    P STTPSPP  
Sbjct: 1   MSSPSPTSPPAPSTTSPLPPTTDAPPPEAPSLSPPPPDAASQPPPATSLPPSTTPSPPPP 60

Query: 61  ---TSSPSPPDVPSSSPPPP-------PTDSTAPPQLPSPT--PPSNSSTPVNPGGNQAP 120
              T SP PPD  + SPPPP       PT S  PP  PS +  PPSNSSTP +PGG+ AP
Sbjct: 61  DSATPSPPPPDSATPSPPPPDSTTPSPPTTSPLPPTAPSSSSPPPSNSSTPTSPGGHDAP 120

Query: 121 PS-----------ETAPSPSTSSGVSAGLVSGVAV-GVVVVVVALIAMCFYLRKKRRRDE 180
           PS           E  PS S SSG SAGLV+GV + GVVVVVVA I +   LRKKRRRDE
Sbjct: 121 PSHSGTPTNPGGNEAPPSFSPSSGASAGLVTGVVIAGVVVVVVAXILLVLCLRKKRRRDE 180

Query: 181 EAYYRPPHPPAPPAAFKDGPYGPQQHHWQSHPPPPGDHIAGAVPKPSPPPASRPPPSPP- 240
           E+YYRPP    PP AFKDGPYG QQHHWQSHPPPP +H+ G VPKPSPPPASRPPPSP  
Sbjct: 181 ESYYRPP----PPGAFKDGPYGLQQHHWQSHPPPPTNHVVGTVPKPSPPPASRPPPSPSP 240

Query: 241 ---------IINSSGGSGSNYSGSENSLAP--------APSSVPLGFSQSSFTYEELAMA 300
                    II SSGGSGS YSGSE S AP        APSS+PLG+SQSSFTYEELAMA
Sbjct: 241 ASRPPPSPAIIYSSGGSGSYYSGSEYSHAPVPAPAPAPAPSSIPLGYSQSSFTYEELAMA 300

Query: 301 TDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHL 360
           TD FSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHL
Sbjct: 301 TDRFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHL 360

Query: 361 VSLVGYCITGSQRLLVYEFVPNNTLEFHLHG----------------------------- 420
           VSLVGYCITGSQRLLVYEFVPNNTLEFHLHG                             
Sbjct: 361 VSLVGYCITGSQRLLVYEFVPNNTLEFHLHGKGRPTMDWPTRLKIALGSAKGLAYLHEDC 420

Query: 421 NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGK 480
           NPKIIHRDIKAANILLDLKFEA VADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGK
Sbjct: 421 NPKIIHRDIKAANILLDLKFEAMVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGK 480

Query: 481 LTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKL 540
           LT+KSDVFS+GVMLLE+ITGRRPVD TQS+MDDGLLDWARPLLLRA E+GNYDGLVDPKL
Sbjct: 481 LTDKSDVFSFGVMLLEMITGRRPVDTTQSYMDDGLLDWARPLLLRAAEDGNYDGLVDPKL 540

Query: 541 RDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSS 587
           RDN+DHNEM RM+ACAAACVRHSARRRPRMSQVVHALEGEASLSDLN+G++PGHSTVYSS
Sbjct: 541 RDNYDHNEMGRMIACAAACVRHSARRRPRMSQVVHALEGEASLSDLNKGVQPGHSTVYSS 600

BLAST of Cp4.1LG04g09960 vs. TAIR 10
Match: AT3G24550.1 (proline extensin-like receptor kinase 1 )

HSP 1 Score: 646.4 bits (1666), Expect = 2.3e-185
Identity = 389/640 (60.78%), Postives = 456/640 (71.25%), Query Frame = 0

Query: 3   SPAPISPPGTNSTSPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPDVP 62
           SP+P SPP  ++T+ PPP   +PPP +       P+S PP P++ + SPP SSP PP +P
Sbjct: 12  SPSPPSPPTNSTTTTPPPAASSPPPTT------TPSSPPPSPSTNSTSPPPSSPLPPSLP 71

Query: 63  SSSPP---PPPTDSTAP-----PQLPSPTPPSNSSTPVNP---------GGNQAPPSETA 122
             SPP    PP    +P     P  PSPT PSN  +P +P         G     PS T 
Sbjct: 72  PPSPPGSLTPPLPQPSPSAPITPSPPSPTTPSNPRSPPSPNQGPPNTPSGSTPRTPSNTK 131

Query: 123 PSP--STSSGVSAGLVSGVAVGVVVVVVALIAMCFYLRKKRRR---DEEAYYRPPHPPAP 182
           PSP   +S G+S G+V G+A+G V ++V L  +C   +KKRRR   DE AYY PP PP+ 
Sbjct: 132 PSPPSDSSDGLSTGVVVGIAIGGVAILVILTLICLLCKKKRRRRHDDEAAYYVPPPPPSG 191

Query: 183 PAAFKDGPYGPQQHHWQSH--PPPPGDHIAGAVPKPSP--PPASRPPPSPPIINSSGGSG 242
           P A   GPYG QQ +WQ      P  +H+  ++P P P  PP   PPP PP    S   G
Sbjct: 192 PKA--GGPYGGQQQYWQQQNASRPSDNHVVTSLPPPKPPSPPRKPPPPPPPPAFMSSSGG 251

Query: 243 SNYSGSENSLAPAPSSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNG 302
           S+YS       P+P  V LGFS+S+FTYEEL+ AT+GFSEANLLGQGGFGYVHKG+LP+G
Sbjct: 252 SDYSDLPVLPPPSPGLV-LGFSKSTFTYEELSRATNGFSEANLLGQGGFGYVHKGILPSG 311

Query: 303 KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNTLE 362
           KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSL+GYC+ G QRLLVYEFVPNN LE
Sbjct: 312 KEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLIGYCMAGVQRLLVYEFVPNNNLE 371

Query: 363 FHLHG-----------------------------NPKIIHRDIKAANILLDLKFEAKVAD 422
           FHLHG                             NPKIIHRDIKA+NIL+D KFEAKVAD
Sbjct: 372 FHLHGKGRPTMEWSTRLKIALGSAKGLSYLHEDCNPKIIHRDIKASNILIDFKFEAKVAD 431

Query: 423 FGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDP 482
           FGLAK++SD NTHVSTRVMGTFGYLAPEYA+SGKLTEKSDVFS+GV+LLELITGRRPVD 
Sbjct: 432 FGLAKIASDTNTHVSTRVMGTFGYLAPEYAASGKLTEKSDVFSFGVVLLELITGRRPVDA 491

Query: 483 TQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARR 542
              ++DD L+DWARPLL RA+E G+++GL D K+ + +D  EMARMVACAAACVRHSARR
Sbjct: 492 NNVYVDDSLVDWARPLLNRASEEGDFEGLADSKMGNEYDREEMARMVACAAACVRHSARR 551

Query: 543 RPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSY-GSSDYDTAQYNEDLKKFRKMALAS 585
           RPRMSQ+V ALEG  SLSDLNEG+RPGHS VYSSY GS+DYDT+QYN+D+ KFRKMAL +
Sbjct: 552 RPRMSQIVRALEGNVSLSDLNEGMRPGHSNVYSSYGGSTDYDTSQYNDDMIKFRKMALGT 611

BLAST of Cp4.1LG04g09960 vs. TAIR 10
Match: AT1G49270.1 (Protein kinase superfamily protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.3e-135
Identity = 343/668 (51.35%), Postives = 413/668 (61.83%), Query Frame = 0

Query: 4   PAPISPPGTN---STSPPP----PTTDAPPPASPSLSPPP--PASSPPP--PNSTTPSPP 63
           P+P SPP +N   +TSPPP     TT  PPP+SP ++PPP     SPPP  P +++    
Sbjct: 17  PSPPSPPSSNDQQTTSPPPSDNQETTSPPPPSSPDIAPPPQQQQESPPPPLPENSSDGSS 76

Query: 64  TSSPSPPDVPSSSPPPPPTDSTAPPQLP----------------------------SPTP 123
           +SSP PP   SS    PP  ST+PPQ                              S TP
Sbjct: 77  SSSPPPPSDSSSQSQSPPPPSTSPPQQSDNNGNKGNNNENNKGNDGSSGDGGNKNMSHTP 136

Query: 124 PSNSSTPVNPGGNQ----APPSETAPSPSTSS-GVSAGLVSGV--AVGVVVVVVALIAMC 183
           P  S T  +   +Q    APP+  + S S+S+ G++ G V G+  A G++ +V+ L+ +C
Sbjct: 137 PPPSKTSDHSSHSQPRSLAPPTSNSGSNSSSNDGLNIGAVIGLVAAAGILFIVMILLCVC 196

Query: 184 FYLRKKRRR--DEEAYYRPPHPPAPPAA----FKDGPYGPQQHHWQSH------PPPPGD 243
            + +KK++   D+  YY     PA        +       QQ H+  +      PPPPG 
Sbjct: 197 CFRKKKKKSKLDQMPYYGSNAYPAGKTGGDQYYNQNAATQQQQHYNQNDHIVNLPPPPGS 256

Query: 244 HIAGAVPKPSPPPAS--RPPPSPPI-------INSSGGSGSNYSGS--ENSLAPAPSSVP 303
                V  P PPP    +P PSPP        +  SG   SN+S      SL P   SV 
Sbjct: 257 MGTNWVSSPPPPPPGNWQPMPSPPAPVSGGANVIQSGEMSSNFSSGPYAPSLPPPHPSVA 316

Query: 304 LGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGERE 363
           LGF+ S+FTYEELA AT GFS+  LLGQGGFGYVHKG+LPNGKE+AVK LKAGSGQGERE
Sbjct: 317 LGFNNSTFTYEELASATQGFSKDRLLGQGGFGYVHKGILPNGKEIAVKSLKAGSGQGERE 376

Query: 364 FQAEVEIISRVHHRHLVSLVGYCI-TGSQRLLVYEFVPNNTLEFHLHG------------ 423
           FQAEVEIISRVHHRHLVSLVGYC   G QRLLVYEF+PN+TLEFHLHG            
Sbjct: 377 FQAEVEIISRVHHRHLVSLVGYCSNAGGQRLLVYEFLPNDTLEFHLHGKSGTVMDWPTRL 436

Query: 424 -----------------NPKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTR 483
                            +PKIIHRDIKA+NILLD  FEAKVADFGLAKLS D NTHVSTR
Sbjct: 437 KIALGSAKGLAYLHEDCHPKIIHRDIKASNILLDHNFEAKVADFGLAKLSQDNNTHVSTR 496

Query: 484 VMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLL 543
           VMGTFGYLAPEYASSGKLTEKSDVFS+GVMLLELITGR PVD     M+D L+DWARPL 
Sbjct: 497 VMGTFGYLAPEYASSGKLTEKSDVFSFGVMLLELITGRGPVD-LSGDMEDSLVDWARPLC 556

Query: 544 LRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASL 571
           +R  ++G Y  LVDP L   ++  EMARMVACAAA VRHS RRRP+MSQ+V  LEG+ASL
Sbjct: 557 MRVAQDGEYGELVDPFLEHQYEPYEMARMVACAAAAVRHSGRRRPKMSQIVRTLEGDASL 616

BLAST of Cp4.1LG04g09960 vs. TAIR 10
Match: AT2G18470.1 (roline-rich extensin-like receptor kinase 4 )

HSP 1 Score: 478.4 bits (1230), Expect = 8.2e-135
Identity = 326/630 (51.75%), Postives = 399/630 (63.33%), Query Frame = 0

Query: 5   APISPPGTNSTSPPPP------TTDAPPPASPSLSPPPP---ASSPPPPNSTTPSPPTSS 64
           +P S P TNSTS P P      TT +PP  SP  SP PP   +SS PPP+ST+P P   +
Sbjct: 4   SPESAPPTNSTSSPSPPSNTNSTTSSPPAPSPP-SPTPPQGDSSSSPPPDSTSP-PAPQA 63

Query: 65  PSPPDVPSSSPPPPPT-------------DSTAPPQLPSPTPPSNSSTPVNPGGNQAPPS 124
           P+PP+  ++SP PP                +  PP   SP  P + S   N G   +PP 
Sbjct: 64  PNPPNSSNNSPSPPSQGGGGERGNGGNNGGNDTPPSRGSPPSPPSRSNGDNGGSRSSPPG 123

Query: 125 ETAPS----PSTSSGVSAG-----------LVSGVAVGVVVVVVALIAMCFYLRKKRRRD 184
           +T  S    P +S G S G           ++ GV VG  ++++ LI +C  LR+K++R 
Sbjct: 124 DTGGSRSDNPPSSGGSSGGGGGGRSNTNTAIIVGVLVGAGLLMIVLIIVC--LRRKKKRK 183

Query: 185 EEAYYRPPHPPAPPAAFKDGPYGPQQH--------HWQSHPPPPGDHIAGAVPKPSPPPA 244
           +  Y      P P    +   YG   +        +W  +         G      P   
Sbjct: 184 DSFY------PEPMKGNQYQYYGNNNNNNASQNYPNWHLNSQGQNQQSTGGWGGGGP--- 243

Query: 245 SRPPPSPPIINSSGGSGSNYSGSENSLAPAPS-SVPLGFSQSSFTYEELAMATDGFSEAN 304
              PP PP + +SG   S YSG    + P PS ++ LGF++S+FTY+ELA AT GF++AN
Sbjct: 244 --SPPPPPRMPTSGEDSSMYSGPSRPVLPPPSPALALGFNKSTFTYQELAAATGGFTDAN 303

Query: 305 LLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCI 364
           LLGQGGFGYVHKGVLP+GKEVAVK LKAGSGQGEREFQAEV+IISRVHHR+LVSLVGYCI
Sbjct: 304 LLGQGGFGYVHKGVLPSGKEVAVKSLKAGSGQGEREFQAEVDIISRVHHRYLVSLVGYCI 363

Query: 365 TGSQRLLVYEFVPNNTLEFHLHG-----------------------------NPKIIHRD 424
              QR+LVYEFVPN TLE+HLHG                             +P+IIHRD
Sbjct: 364 ADGQRMLVYEFVPNKTLEYHLHGKNLPVMEFSTRLRIALGAAKGLAYLHEDCHPRIIHRD 423

Query: 425 IKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF 484
           IK+ANILLD  F+A VADFGLAKL+SD NTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF
Sbjct: 424 IKSANILLDFNFDAMVADFGLAKLTSDNNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVF 483

Query: 485 SYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNE 544
           SYGVMLLELITG+RPVD + + MDD L+DWARPL+ RA E+GN++ L D +L  N++  E
Sbjct: 484 SYGVMLLELITGKRPVDNSIT-MDDTLVDWARPLMARALEDGNFNELADARLEGNYNPQE 543

Query: 545 MARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSYG-SSDYD 559
           MARMV CAAA +RHS R+RP+MSQ+V ALEGE SL  LNEG++PGHS VY S G SSDY 
Sbjct: 544 MARMVTCAAASIRHSGRKRPKMSQIVRALEGEVSLDALNEGVKPGHSNVYGSLGASSDYS 603

BLAST of Cp4.1LG04g09960 vs. TAIR 10
Match: AT4G34440.1 (Protein kinase superfamily protein )

HSP 1 Score: 466.1 bits (1198), Expect = 4.2e-131
Identity = 324/664 (48.80%), Postives = 408/664 (61.45%), Query Frame = 0

Query: 2   SSPAPISPPGTNSTSPPPPTTDAPPP-ASPSLSPP--PPASSPPPPNSTTPSPPTSSPSP 61
           +SP+  S P T  +SPPP +  APPP  S S SPP  PP     PP S + SPP  +   
Sbjct: 24  TSPSNESSPPTPPSSPPPSSISAPPPDISASFSPPPAPPTQETSPPTSPSSSPPVVANPS 83

Query: 62  PDVPSSSPPPPPTDST-----APPQLPS------PTPPS-----------------NSST 121
           P  P +  PP P  ST     APPQ PS      PTPPS                 + ST
Sbjct: 84  PQTPENPSPPAPEGSTPVTPPAPPQTPSNQSPERPTPPSPGANDDRNRTNGGNNNRDGST 143

Query: 122 PVNP---------GGNQAPPSETAP-----SPSTSSG----VSAGLVSGVAVGVVVVVVA 181
           P  P         GG+ +PP   +P        +SSG     + GL+ GV VG  ++++ 
Sbjct: 144 PSPPSSGNRTSGDGGSPSPPRSISPPQNSGDSDSSSGNHPQANIGLIIGVLVGAGLLLLL 203

Query: 182 LIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKDGPY-GPQQHHWQSHPPPPGDHIAGAV 241
            + +C    +K+++         +       + + PY G    +   +   P DH+    
Sbjct: 204 AVCICICCNRKKKKKSPQVNHMHY-------YNNNPYGGAPSGNGGYYKGTPQDHVVNMA 263

Query: 242 PKPSPPPASRPPPSPPIINSSGGSGSNYSGSENSLAPAPSSVPLGFSQSSFTYEELAMAT 301
            +       + P S P  ++S  +G        +  P+P +  LG +QS+FTY+EL++AT
Sbjct: 264 GQGGGNWGPQQPVSGPHSDASNLTG-------RTAIPSPQAATLGHNQSTFTYDELSIAT 323

Query: 302 DGFSEANLLGQGGFGYVHKGVLPNGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLV 361
           +GF+++NLLGQGGFGYVHKGVLP+GKEVAVK LK GSGQGEREFQAEV+IISRVHHRHLV
Sbjct: 324 EGFAQSNLLGQGGFGYVHKGVLPSGKEVAVKSLKLGSGQGEREFQAEVDIISRVHHRHLV 383

Query: 362 SLVGYCITGSQRLLVYEFVPNNTLEFHLHG-----------------------------N 421
           SLVGYCI+G QRLLVYEF+PNNTLEFHLHG                             +
Sbjct: 384 SLVGYCISGGQRLLVYEFIPNNTLEFHLHGKGRPVLDWPTRVKIALGSARGLAYLHEDCH 443

Query: 422 PKIIHRDIKAANILLDLKFEAKVADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKL 481
           P+IIHRDIKAANILLD  FE KVADFGLAKLS D  THVSTRVMGTFGYLAPEYASSGKL
Sbjct: 444 PRIIHRDIKAANILLDFSFETKVADFGLAKLSQDNYTHVSTRVMGTFGYLAPEYASSGKL 503

Query: 482 TEKSDVFSYGVMLLELITGRRPVDPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLR 541
           ++KSDVFS+GVMLLELITGR P+D T   M+D L+DWARPL L+A ++G+Y+ L DP+L 
Sbjct: 504 SDKSDVFSFGVMLLELITGRPPLDLTGE-MEDSLVDWARPLCLKAAQDGDYNQLADPRLE 563

Query: 542 DNHDHNEMARMVACAAACVRHSARRRPRMSQVVHALEGEASLSDLNEGIRPGHSTVYSSY 585
            N+ H EM +M +CAAA +RHSARRRP+MSQ+V ALEG+ S+ DL+EG RPG ST Y S 
Sbjct: 564 LNYSHQEMVQMASCAAAAIRHSARRRPKMSQIVRALEGDMSMDDLSEGTRPGQST-YLSP 623

BLAST of Cp4.1LG04g09960 vs. TAIR 10
Match: AT3G18810.1 (Protein kinase superfamily protein )

HSP 1 Score: 459.9 bits (1182), Expect = 3.0e-129
Identity = 324/687 (47.16%), Postives = 401/687 (58.37%), Query Frame = 0

Query: 16  SPPPPTTDAPPPASPSLSPPPPASSPPPPNSTTPSPPTSSPSPPDVPSSSPPPPPTDSTA 75
           SPP PT     P SPS S     SSPPP +S++PSPP + P P D  + SP PP +DS +
Sbjct: 10  SPPSPT-----PPSPSSSDNQQQSSPPPSDSSSPSPP-APPPPDDSSNGSPQPPSSDSQS 69

Query: 76  PPQLP------------------------------------------------------- 135
           PP                                                          
Sbjct: 70  PPSPQGNNNNDGNNGNNNNDNNNNNNGNNNNDNNNGNNKDNNNNGNNNNGNNNNGNDNNG 129

Query: 136 ----------------------SPTPPSNSSTPVNPGGNQAPPSETAPSPSTSSGVSA-- 195
                                 SP PPS +S   +P    +PP   AP  S+  G ++  
Sbjct: 130 NNNNGNNNDNNNQNNGGGSNNRSPPPPSRNSDRNSP----SPPRALAPPRSSGGGSNSSG 189

Query: 196 ----------GLVSGVAVGVVVVVVALIAMCFYLRKKRRRDEEAYYRPPHPPAPPAAFKD 255
                     G+V+G   G++ +V+ L  +C   +KK++     Y    +       ++ 
Sbjct: 190 NNEPNTAAIVGIVAG--AGLLFLVMILFCVCCCRKKKKKHQMPYYAGNGYATGKGDQYQQ 249

Query: 256 GPYGPQQHH----WQSHPPPPGDHIAGAVPKPSPPPA---SRPPPSPPIINSSGGS---- 315
             Y  Q  H     Q +P   G++     P P PP +   S PPP PP+     G+    
Sbjct: 250 QQYNNQSDHVMNLSQQYPGSNGNNNWMNSPPPPPPGSWQPSPPPPPPPVSGGMNGNSSDF 309

Query: 316 GSNYSGSENSLAPAP-SSVPLGFSQSSFTYEELAMATDGFSEANLLGQGGFGYVHKGVLP 375
            SNYSG      P P  SV LGF++S+FTY+ELA AT GFS++ LLGQGGFGYVHKG+LP
Sbjct: 310 SSNYSGPHGPSVPPPHPSVALGFNKSTFTYDELAAATQGFSQSRLLGQGGFGYVHKGILP 369

Query: 376 NGKEVAVKQLKAGSGQGEREFQAEVEIISRVHHRHLVSLVGYCITGSQRLLVYEFVPNNT 435
           NGKE+AVK LKAGSGQGEREFQAEV+IISRVHHR LVSLVGYCI G QR+LVYEF+PN+T
Sbjct: 370 NGKEIAVKSLKAGSGQGEREFQAEVDIISRVHHRFLVSLVGYCIAGGQRMLVYEFLPNDT 429

Query: 436 LEFHLHG-----------------------------NPKIIHRDIKAANILLDLKFEAKV 495
           LEFHLHG                             +P+IIHRDIKA+NILLD  FEAKV
Sbjct: 430 LEFHLHGKSGKVLDWPTRLKIALGSAKGLAYLHEDCHPRIIHRDIKASNILLDESFEAKV 489

Query: 496 ADFGLAKLSSDVNTHVSTRVMGTFGYLAPEYASSGKLTEKSDVFSYGVMLLELITGRRPV 555
           ADFGLAKLS D  THVSTR+MGTFGYLAPEYASSGKLT++SDVFS+GVMLLEL+TGRRPV
Sbjct: 490 ADFGLAKLSQDNVTHVSTRIMGTFGYLAPEYASSGKLTDRSDVFSFGVMLLELVTGRRPV 549

Query: 556 DPTQSFMDDGLLDWARPLLLRATENGNYDGLVDPKLRDNHDHNEMARMVACAAACVRHSA 571
           D T   M+D L+DWARP+ L A ++G+Y  LVDP+L + ++ +EMA+MVACAAA VRHSA
Sbjct: 550 DLTGE-MEDSLVDWARPICLNAAQDGDYSELVDPRLENQYEPHEMAQMVACAAAAVRHSA 609

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LV483.2e-18460.78Proline-rich receptor-like protein kinase PERK1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9LK035.0e-15357.17Proline-rich receptor-like protein kinase PERK2 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9XI961.8e-13451.35Proline-rich receptor-like protein kinase PERK7 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZNQ81.2e-13351.75Proline-rich receptor-like protein kinase PERK4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q8GX236.0e-13048.80Proline-rich receptor-like protein kinase PERK5 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
XP_023529433.10.095.29proline-rich receptor-like protein kinase PERK1 [Cucurbita pepo subsp. pepo][more]
KAG6587962.10.094.64Proline-rich receptor-like protein kinase PERK1, partial [Cucurbita argyrosperma... [more]
XP_022933488.10.094.32proline-rich receptor-like protein kinase PERK1 [Cucurbita moschata][more]
XP_023007117.10.093.34proline-rich receptor-like protein kinase PERK1 [Cucurbita maxima][more]
XP_038879967.10.083.60proline-rich receptor-like protein kinase PERK1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F4W30.094.32proline-rich receptor-like protein kinase PERK1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KXQ40.093.34proline-rich receptor-like protein kinase PERK1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A1S3CJ370.082.39proline-rich receptor-like protein kinase PERK1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1JJF01.70e-31173.23proline-rich receptor-like protein kinase PERK1 isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1JHF71.13e-31075.83proline-rich receptor-like protein kinase PERK1 isoform X2 OS=Cucurbita maxima O... [more]
Match NameE-valueIdentityDescription
AT3G24550.12.3e-18560.78proline extensin-like receptor kinase 1 [more]
AT1G49270.11.3e-13551.35Protein kinase superfamily protein [more]
AT2G18470.18.2e-13551.75roline-rich extensin-like receptor kinase 4 [more]
AT4G34440.14.2e-13148.80Protein kinase superfamily protein [more]
AT3G18810.13.0e-12947.16Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 254..498
e-value: 3.6E-24
score: 96.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 338..426
e-value: 4.4E-23
score: 82.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 254..504
score: 26.689951
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 336..510
e-value: 9.9E-50
score: 170.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 226..329
e-value: 3.5E-36
score: 125.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 90..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..115
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..231
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..587
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..575
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..209
NoneNo IPR availablePANTHERPTHR47982PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 12..342
NoneNo IPR availablePANTHERPTHR47982:SF12SERINE-THREONINE/TYROSINE-PROTEIN KINASE CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 12..342
NoneNo IPR availablePANTHERPTHR47982PROLINE-RICH RECEPTOR-LIKE PROTEIN KINASE PERK4coord: 342..577
NoneNo IPR availablePANTHERPTHR47982:SF12SERINE-THREONINE/TYROSINE-PROTEIN KINASE CATALYTIC DOMAIN-CONTAINING PROTEIN-RELATEDcoord: 342..577
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 260..282
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 345..357
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 239..498

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g09960.1Cp4.1LG04g09960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity