Cp4.1LG04g09400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g09400 vs. ExPASy Swiss-Prot
Match: Q9SLN8 (2-alkenal reductase (NADP(+)-dependent) OS=Nicotiana tabacum OX=4097 GN=DBR PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.5e-82
Identity = 157/343 (45.77%), Postives = 223/343 (65.01%), Query Frame = 0

Query: 2   EVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSH 61
           EV N+ V +++++  +P ESD E+K+  + + V  G +D+VVKNLY+S DPY  +RM+  
Sbjct: 4   EVSNKQVILKNYVTGYPKESDMEIKNVTIKLKVPEGSNDVVVKNLYLSCDPYMRSRMR-- 63

Query: 62  SSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD----------- 121
              +   S+  S  P  PI  YGV +VL SG+  F KGDLV G+  W +           
Sbjct: 64  ---KIEGSYVESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITPTQTLF 123

Query: 122 -------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLHG 181
                              G+TAYAG  EV   K+GETVFVSAASG+VG L GQ AK+ G
Sbjct: 124 KIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLG 183

Query: 182 CYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLEA 241
           CYVVGSAGS EKV LLK K GFDEAFNYK+E+DL++ L+RYFPDGID+YF+NVGG+ML+A
Sbjct: 184 CYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDA 243

Query: 242 AVANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAMA 301
            + N+K +GR+A+CG+IS+Y     +    +  L+ KRI++EGFL  D+ ++Y  ++ M 
Sbjct: 244 VLVNMKLYGRIAVCGMISQYNLEQTEGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMV 303

Query: 302 SQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
              + +GK+  +EDV+ G+++ P+A +GLF G NIGK++V ++
Sbjct: 304 IPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMVS 341

BLAST of Cp4.1LG04g09400 vs. ExPASy Swiss-Prot
Match: Q39172 (NADPH-dependent oxidoreductase 2-alkenal reductase OS=Arabidopsis thaliana OX=3702 GN=AER PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.8e-80
Identity = 150/345 (43.48%), Postives = 220/345 (63.77%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           M   N+ V ++ ++  FP ESDF+  +  + + V  G + ++VKNLY+S DPY   R++ 
Sbjct: 1   MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYM--RIRM 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
                 T++ A +  P QPI  YGV  ++ SG+ D+ KGDL+ G++ W +          
Sbjct: 61  GKPDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHA 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                G+TAYAG +EV   KEGETV+VSAASG+VG L GQLAK+
Sbjct: 121 HFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKM 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
            GCYVVGSAGS EKV LLK K GFD+AFNYK+E DLT+ L+R FP+GID+YF+NVGG+ML
Sbjct: 181 MGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKML 240

Query: 241 EAAVANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIA 300
           +A + N+   GR+A+CG+IS+Y   +++    + +++YKRI+++GF+  DF + YS F+ 
Sbjct: 241 DAVLVNMNMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVSDFYDKYSKFLE 300

Query: 301 MASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
               ++  GKI  +EDV+ G++  P A +GLF G N+GK++V +A
Sbjct: 301 FVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA 343

BLAST of Cp4.1LG04g09400 vs. ExPASy Swiss-Prot
Match: Q39173 (NADP-dependent alkenal double bond reductase P2 OS=Arabidopsis thaliana OX=3702 GN=P2 PE=2 SV=2)

HSP 1 Score: 288.5 bits (737), Expect = 9.1e-77
Identity = 147/341 (43.11%), Postives = 213/341 (62.46%), Query Frame = 0

Query: 5   NRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSHSSS 64
           N+ V  + H+  FP ESDF   +  + + V  G   ++VKNLY+S DPY  +RM      
Sbjct: 4   NKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRM---GKP 63

Query: 65  QKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD-------------- 124
             +S+ A +  P +PI  YGV  V+ SG+ D+ KGDL+ G++ W +              
Sbjct: 64  DPSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKI 123

Query: 125 -----------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLHGCY 184
                            G+TAYAG +EV   K+GETV+VSAASG+VG L GQ AK+ GCY
Sbjct: 124 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCY 183

Query: 185 VVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLEAAV 244
           VVGSAGS EKV LLK K GFD+AFNYK+E DL++ L+R FP GID+YF+NVGG+ML+A +
Sbjct: 184 VVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLSAALKRCFPKGIDMYFENVGGKMLDAVL 243

Query: 245 ANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAMASQ 304
            N+   GR+A+CG+IS+Y   +++    + +++YKRI+++GF+  DF + Y  F+ +   
Sbjct: 244 LNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVADFYDKYPKFLELVLP 303

Query: 305 YLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
            +  GKI  +EDV+ G++  P A +GLF G N+GK++V +A
Sbjct: 304 RIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIA 341

BLAST of Cp4.1LG04g09400 vs. ExPASy Swiss-Prot
Match: Q6WAU0 ((+)-pulegone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.8e-69
Identity = 140/344 (40.70%), Postives = 214/344 (62.21%), Query Frame = 0

Query: 3   VMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDD-IVVKNLYVSIDPYQINRMKSH 62
           VMN+ + + ++I+    +SD  L++  + + +  GC+  I+VKNLY+S++PY I RM   
Sbjct: 2   VMNKQIVLNNYINGSLKQSDLALRTSTICMEIPDGCNGAILVKNLYLSVNPYLILRMGKL 61

Query: 63  SSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAG-LLHWAD---------- 122
              Q       SI P   I +YGV +VL S +  + KG+L+ G    W +          
Sbjct: 62  DIPQ-----FDSILPGSTIVSYGVSKVLDSTHPSYEKGELIWGSQAGWEEYTLIQNPYNL 121

Query: 123 --------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLH 182
                               G+TAYAG FE+   K+GETVFV+AA+GSVG L GQ AK+ 
Sbjct: 122 FKIQDKDVPLSYYVGILGMPGMTAYAGFFEICSPKKGETVFVTAAAGSVGQLVGQFAKMF 181

Query: 183 GCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLE 242
           GCYVVGSAGS EKV LLK K GFD+AFNYK+E D  + L+R+FP+GID+YFDNVGG+MLE
Sbjct: 182 GCYVVGSAGSKEKVDLLKNKFGFDDAFNYKEESDYDTALKRHFPEGIDIYFDNVGGKMLE 241

Query: 243 AAVANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 302
           A + N++  GR+A+CG++S+Y+    +    +L L+ K+I+++GF+  D+ ++Y  F+ M
Sbjct: 242 AVINNMRVHGRIAVCGMVSQYSLKQPEGVHNLLKLIPKQIRMQGFVVVDYYHLYPKFLEM 301

Query: 303 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
               +  GK+  +ED+S G+++ PSA +G++ G N+G ++V ++
Sbjct: 302 VLPRIKEGKVTYVEDISEGLESAPSALLGVYVGRNVGNQVVAVS 340

BLAST of Cp4.1LG04g09400 vs. ExPASy Swiss-Prot
Match: O34812 (Putative NADP-dependent oxidoreductase YfmJ OS=Bacillus subtilis (strain 168) OX=224308 GN=yfmJ PE=2 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.9e-54
Identity = 123/329 (37.39%), Postives = 184/329 (55.93%), Query Frame = 0

Query: 18  PVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSHSSSQKTSSFATSIKPA 77
           PV  DF  ++   I   +P   +++VK LYVS+DPY   RM      Q T S+       
Sbjct: 18  PVHEDFRFET---IPVPEPKQGEVLVKTLYVSVDPYMRGRM------QDTKSYVEPFALD 77

Query: 78  QPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD--------------------------- 137
           + +    + EV++ GN    KGD+V G L W +                           
Sbjct: 78  KALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVSESALRKIDTSLAPASAYLGILG 137

Query: 138 --GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLHGCYVVGSAGSDEKVALLK 197
             GLTAY GL ++ + KEGETV VS A+G+VGS  GQ+AK+ G  VVG AGSDEK+  LK
Sbjct: 138 MTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLK 197

Query: 198 EKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLEAAVANLKAFGRVALCGVI 257
           ++L FDEA NYK   D+   L+   PDG+DVYFDNVGG + +A +  L  F R+ +CG I
Sbjct: 198 QELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAI 257

Query: 258 SEYTSSKKA---APKMLD-LVYKRIKVEGFLAGDFLNVYSDFIAMASQYLGSGKIESLED 314
           S Y +  +A    P++   L+  +  ++GF+  D+ + +S+     +++L +GK+   E 
Sbjct: 258 SSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEET 317

BLAST of Cp4.1LG04g09400 vs. NCBI nr
Match: XP_023529328.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1526), Expect = 3.21e-212
Identity = 313/345 (90.72%), Postives = 314/345 (91.01%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAEYSIVEAQTSL 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRKIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
           ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV
Sbjct: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 345

BLAST of Cp4.1LG04g09400 vs. NCBI nr
Match: KAG7021788.1 (2-alkenal reductase (NADP(+)-dependent) [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580 bits (1496), Expect = 1.20e-207
Identity = 308/345 (89.28%), Postives = 310/345 (89.86%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVK GCDDIVVKNLYVSIDPYQINRMKS
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGLLHWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
            SQYLGS KIESLEDVSIGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 301 VSQYLGSRKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKIVKLAV 345

BLAST of Cp4.1LG04g09400 vs. NCBI nr
Match: XP_022934032.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita moschata])

HSP 1 Score: 578 bits (1489), Expect = 1.39e-206
Identity = 306/345 (88.70%), Postives = 309/345 (89.57%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVK GCDDIVVKNLYVSIDPYQINRMKS
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGLLHWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKV LLKEKLGFDEAFNYKQE+DLTSTLERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVGLLKEKLGFDEAFNYKQERDLTSTLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
            SQYLGS KIESLEDVSIGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 301 VSQYLGSRKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKIVKLAV 345

BLAST of Cp4.1LG04g09400 vs. NCBI nr
Match: XP_022972110.1 (2-alkenal reductase (NADP(+)-dependent)-like [Cucurbita maxima])

HSP 1 Score: 576 bits (1485), Expect = 5.67e-206
Identity = 304/345 (88.12%), Postives = 312/345 (90.43%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIR++IDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMK+
Sbjct: 1   MEVMNRVVAIRNYIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKT 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGL+HWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLVHWAEYSIVKAHTNM 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRKIDSMGFPLTNHLGILGLSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTS+LERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSSLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLK FGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKPFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
           ASQYLGSGKIESLEDV+IGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 301 ASQYLGSGKIESLEDVTIGVDTIPSAFIGLFKGDNIGKKIVKLAV 345

BLAST of Cp4.1LG04g09400 vs. NCBI nr
Match: KAG6587901.1 (Heme oxygenase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580 bits (1496), Expect = 7.79e-203
Identity = 308/345 (89.28%), Postives = 310/345 (89.86%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVK GCDDIVVKNLYVSIDPYQINRMKS
Sbjct: 316 MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 375

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGLLHWA+          
Sbjct: 376 HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 435

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 436 LRQIDSMGLPLTNHIGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 495

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML
Sbjct: 496 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 555

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 556 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 615

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
            SQYLGS KIESLEDVSIGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 616 VSQYLGSRKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKIVKLAV 660

BLAST of Cp4.1LG04g09400 vs. ExPASy TrEMBL
Match: A0A6J1F1I6 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita moschata OX=3662 GN=LOC111441259 PE=4 SV=1)

HSP 1 Score: 578 bits (1489), Expect = 6.75e-207
Identity = 306/345 (88.70%), Postives = 309/345 (89.57%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVK GCDDIVVKNLYVSIDPYQINRMKS
Sbjct: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKAGCDDIVVKNLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGLLHWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLLHWAEYSIVKAQTSL 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRQIDSMGLPLTNHLGILGFSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKV LLKEKLGFDEAFNYKQE+DLTSTLERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVGLLKEKLGFDEAFNYKQERDLTSTLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
            SQYLGS KIESLEDVSIGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 301 VSQYLGSRKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKIVKLAV 345

BLAST of Cp4.1LG04g09400 vs. ExPASy TrEMBL
Match: A0A6J1I7M4 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucurbita maxima OX=3661 GN=LOC111470739 PE=4 SV=1)

HSP 1 Score: 576 bits (1485), Expect = 2.75e-206
Identity = 304/345 (88.12%), Postives = 312/345 (90.43%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEVMNRVVAIR++IDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMK+
Sbjct: 1   MEVMNRVVAIRNYIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKT 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADF KGDLVAGL+HWA+          
Sbjct: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFKKGDLVAGLVHWAEYSIVKAHTNM 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL
Sbjct: 121 LRKIDSMGFPLTNHLGILGLSGLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTS+LERYFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSSLERYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAAVANLK FGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM
Sbjct: 241 EAAVANLKPFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLAV 314
           ASQYLGSGKIESLEDV+IGVDTIPSAFIGLFKGDNIGKK+VKLAV
Sbjct: 301 ASQYLGSGKIESLEDVTIGVDTIPSAFIGLFKGDNIGKKIVKLAV 345

BLAST of Cp4.1LG04g09400 vs. ExPASy TrEMBL
Match: A0A1S3B9C7 (2-alkenal reductase (NADP(+)-dependent)-like OS=Cucumis melo OX=3656 GN=LOC103487456 PE=4 SV=1)

HSP 1 Score: 480 bits (1236), Expect = 2.26e-168
Identity = 246/344 (71.51%), Postives = 276/344 (80.23%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEV NR+V  R  IDEFPVESDFE+K E  +V VK G DDI+VKNLYVSIDPYQINRMKS
Sbjct: 1   MEVRNRIVTTRKAIDEFPVESDFEVKCEDFVVLVKSGSDDIIVKNLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
            SS+ KTS FAT I P+QPIDA GVGEVLASGN +F +GD+V G+ HWA+          
Sbjct: 61  FSSTHKTSDFATPIIPSQPIDALGVGEVLASGNPEFKEGDIVIGITHWAEYSVVKAHTNL 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                GLTAYAGLFEVAK+KEGETVFVSAASGSVGSL GQ AKL
Sbjct: 121 LRKLETLGFPLTHHLGILGFSGLTAYAGLFEVAKIKEGETVFVSAASGSVGSLVGQFAKL 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
           HGCYVVG AGSD+KV LLKEK GFD AFNYKQEKDLT+TLE+YFPDGIDVYFDNVGGEML
Sbjct: 181 HGCYVVGCAGSDQKVTLLKEKFGFDNAFNYKQEKDLTATLEKYFPDGIDVYFDNVGGEML 240

Query: 241 EAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           EAA+AN+K FGRVA+CGVISEYT+SKKA P M+DLVYKRIKVEGFLAGDFL+++ +F++ 
Sbjct: 241 EAAIANMKPFGRVAVCGVISEYTNSKKAVPNMIDLVYKRIKVEGFLAGDFLDMFPNFVSK 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 313
            SQYL SGKIE LED+S+GV+ IPSAFIGLFKGDNIGKK+VK A
Sbjct: 301 VSQYLHSGKIEPLEDISVGVENIPSAFIGLFKGDNIGKKIVKFA 344

BLAST of Cp4.1LG04g09400 vs. ExPASy TrEMBL
Match: A0A6J1CVC7 (2-alkenal reductase (NADP(+)-dependent)-like OS=Momordica charantia OX=3673 GN=LOC111014607 PE=4 SV=1)

HSP 1 Score: 479 bits (1232), Expect = 9.52e-168
Identity = 245/345 (71.01%), Postives = 276/345 (80.00%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEV NR VA R HID+FPVESDFEL SEA+++SV PG DDI++K LYVSIDPYQINRMKS
Sbjct: 1   MEVSNRFVATRTHIDDFPVESDFELNSEAVVLSVAPGSDDIILKTLYVSIDPYQINRMKS 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
           H+SS KTSSFAT I P QPID +G  EV+ASGN DF +GDLV GL++WA+          
Sbjct: 61  HNSSHKTSSFATPISPGQPIDGFGAAEVVASGNPDFRRGDLVVGLINWAEYTVVKAQNRL 120

Query: 121 ----------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAK 180
                                 GLTA+ GLF VAK K+GE VFVSAASGSVG+L GQ AK
Sbjct: 121 LRKLDTALEFPITHHLGIFGLSGLTAHVGLFVVAKAKKGEKVFVSAASGSVGNLVGQFAK 180

Query: 181 LHGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEM 240
           LHGCYV+G AGSD+KV +LKEKLGFD+AFNYKQEKDLTSTLE+YFPDGIDVYFDNVGG M
Sbjct: 181 LHGCYVIGCAGSDQKVTVLKEKLGFDDAFNYKQEKDLTSTLEKYFPDGIDVYFDNVGGVM 240

Query: 241 LEAAVANLKAFGRVALCGVISEYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIA 300
           LEAAVANL  FGRVALCGVISEYT+SKKAAP MLDLVYKRIK+EGFLAGD  +VYSDFIA
Sbjct: 241 LEAAVANLNTFGRVALCGVISEYTNSKKAAPNMLDLVYKRIKIEGFLAGDHFDVYSDFIA 300

Query: 301 MASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 313
            AS Y+ SGKIE+LEDVS+G+ +IPSAF+GLFKGDNIGKK+VK A
Sbjct: 301 KASDYIRSGKIETLEDVSMGIHSIPSAFVGLFKGDNIGKKIVKFA 345

BLAST of Cp4.1LG04g09400 vs. ExPASy TrEMBL
Match: A0A5J5AAJ5 (PKS_ER domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_035106 PE=4 SV=1)

HSP 1 Score: 422 bits (1085), Expect = 2.32e-145
Identity = 214/343 (62.39%), Postives = 261/343 (76.09%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           MEV N+ +AIR HID  P ESDFELK+EAL VS++PGC+D+VVK LYVSIDPYQINRMK 
Sbjct: 1   MEVTNKYIAIRSHIDGAPQESDFELKTEALSVSIEPGCNDVVVKTLYVSIDPYQINRMKK 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
            SSSQKTS+FA +I P Q IDA+GVG V+ASG+  F K DLVAG++ W +          
Sbjct: 61  FSSSQKTSTFAVAINPGQAIDAFGVGRVVASGHPGFEKDDLVAGVISWGEYSIVRGGNML 120

Query: 121 --------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLH 180
                               GLTAY G F +   K+GE VFVSAASGSVG+L GQ AKL 
Sbjct: 121 NKLDSMGFPLSYHVGILGFSGLTAYGGFFHICNPKKGEKVFVSAASGSVGNLVGQYAKLF 180

Query: 181 GCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLE 240
           GCYVVG AGS +KV LLKEKLGFD+AFNYK+E DL STL+RYFP+GID+YFDNVG EMLE
Sbjct: 181 GCYVVGCAGSQKKVELLKEKLGFDDAFNYKEETDLKSTLKRYFPEGIDIYFDNVGAEMLE 240

Query: 241 AAVANLKAFGRVALCGVISEYTSSKK-AAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAM 300
           AAVAN+  +GRVA+CGVISEYT +KK AAP M+D+VYKRIK+ GFLAGD +N+Y+DF++ 
Sbjct: 241 AAVANMNTYGRVAVCGVISEYTDTKKRAAPDMIDVVYKRIKIHGFLAGDLMNLYADFLST 300

Query: 301 ASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKL 312
              YL +GKI+++ED+S G++ +PSAFIGLF GDN+GKK+VK+
Sbjct: 301 TIDYLRTGKIQAIEDISHGLENVPSAFIGLFCGDNVGKKIVKV 343

BLAST of Cp4.1LG04g09400 vs. TAIR 10
Match: AT5G16970.1 (alkenal reductase )

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-81
Identity = 150/345 (43.48%), Postives = 220/345 (63.77%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           M   N+ V ++ ++  FP ESDF+  +  + + V  G + ++VKNLY+S DPY   R++ 
Sbjct: 1   MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPEGTNSVLVKNLYLSCDPYM--RIRM 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
                 T++ A +  P QPI  YGV  ++ SG+ D+ KGDL+ G++ W +          
Sbjct: 61  GKPDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHA 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                G+TAYAG +EV   KEGETV+VSAASG+VG L GQLAK+
Sbjct: 121 HFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKM 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
            GCYVVGSAGS EKV LLK K GFD+AFNYK+E DLT+ L+R FP+GID+YF+NVGG+ML
Sbjct: 181 MGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKML 240

Query: 241 EAAVANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIA 300
           +A + N+   GR+A+CG+IS+Y   +++    + +++YKRI+++GF+  DF + YS F+ 
Sbjct: 241 DAVLVNMNMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVSDFYDKYSKFLE 300

Query: 301 MASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
               ++  GKI  +EDV+ G++  P A +GLF G N+GK++V +A
Sbjct: 301 FVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA 343

BLAST of Cp4.1LG04g09400 vs. TAIR 10
Match: AT5G17000.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 291.2 bits (744), Expect = 1.0e-78
Identity = 148/345 (42.90%), Postives = 217/345 (62.90%), Query Frame = 0

Query: 1   MEVMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKS 60
           M   N+ V +++++  FP ESDF+ K+  +   +  G + ++VKNLY+S DPY   R++ 
Sbjct: 1   MTATNKQVVLKNYVSGFPKESDFDFKTTTVEFKLPGGSNSVLVKNLYLSCDPYM--RIRM 60

Query: 61  HSSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD---------- 120
                 T++ A +  P +PI  YGV  V+ SG+ D+ KGDL+ G++ W +          
Sbjct: 61  GKPDPSTAALAQAYAPGKPIFGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMTHM 120

Query: 121 ---------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKL 180
                                G+TAYAG +EV   KEGETV+VSAASG+VG L GQ AK+
Sbjct: 121 HFKIQHTDIPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQFAKM 180

Query: 181 HGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEML 240
            GCYVVGSAGS EKV LLK K GFD+AFNYK+E DL++ L+R FP GID+YF+NVGG+ML
Sbjct: 181 MGCYVVGSAGSTEKVDLLKTKFGFDDAFNYKEEPDLSAALKRCFPTGIDIYFENVGGKML 240

Query: 241 EAAVANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIA 300
           +A + N+   GR+A+CG+IS+Y   +++    + +++YKRI+++GF   DF   YS F+ 
Sbjct: 241 DAVLLNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFAVFDFYEKYSKFLD 300

Query: 301 MASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
               ++  GKI  +EDV+ G++  P A +GLF G N+GK++V +A
Sbjct: 301 FVLPHIKEGKITYVEDVADGLEKGPEALVGLFHGKNVGKQVVVIA 343

BLAST of Cp4.1LG04g09400 vs. TAIR 10
Match: AT5G16990.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 288.5 bits (737), Expect = 6.5e-78
Identity = 147/341 (43.11%), Postives = 213/341 (62.46%), Query Frame = 0

Query: 5   NRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSHSSS 64
           N+ V  + H+  FP ESDF   +  + + V  G   ++VKNLY+S DPY  +RM      
Sbjct: 4   NKQVIFKDHVSGFPKESDFNFTTTTVELRVPEGSKSVLVKNLYLSCDPYMRSRM---GKP 63

Query: 65  QKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD-------------- 124
             +S+ A +  P +PI  YGV  V+ SG+ D+ KGDL+ G++ W +              
Sbjct: 64  DPSSALAQAYAPGKPIYGYGVSRVIESGHPDYKKGDLLWGIVGWEEYSVITPMAHMHFKI 123

Query: 125 -----------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLHGCY 184
                            G+TAYAG +EV   K+GETV+VSAASG+VG L GQ AK+ GCY
Sbjct: 124 QHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKKGETVYVSAASGAVGQLVGQFAKMMGCY 183

Query: 185 VVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLEAAV 244
           VVGSAGS EKV LLK K GFD+AFNYK+E DL++ L+R FP GID+YF+NVGG+ML+A +
Sbjct: 184 VVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLSAALKRCFPKGIDMYFENVGGKMLDAVL 243

Query: 245 ANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAMASQ 304
            N+   GR+A+CG+IS+Y   +++    + +++YKRI+++GF+  DF + Y  F+ +   
Sbjct: 244 LNMNPHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIRIQGFVVADFYDKYPKFLELVLP 303

Query: 305 YLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
            +  GKI  +EDV+ G++  P A +GLF G N+GK++V +A
Sbjct: 304 RIKEGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVIA 341

BLAST of Cp4.1LG04g09400 vs. TAIR 10
Match: AT1G65560.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 285.0 bits (728), Expect = 7.1e-77
Identity = 146/347 (42.07%), Postives = 215/347 (61.96%), Query Frame = 0

Query: 3   VMNRVVAIRHHIDEFPVESDFELK-SEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSH 62
           V N+ V +++++D  P E+D E+K  E + +    G    +VKNLY+S DPY   RM+  
Sbjct: 7   VENKKVILKNYVDGIPTETDMEVKLGETIELKAPKGSSCFLVKNLYLSCDPYMRGRMRDF 66

Query: 63  SSSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD----------- 122
                  S+     P Q I+ +G+  V+ S + ++  GD+V+G++ W +           
Sbjct: 67  H-----GSYLPPFVPGQRIEGFGLARVIDSDDTNYKPGDIVSGIIGWEEYSLLRSSDNLQ 126

Query: 123 ----------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAK 182
                                 G TAYAG  E+   K+G++VFVSAA G+VG L GQLAK
Sbjct: 127 LRNIQLDDDIPLSYHLGLLGMAGFTAYAGFNEICCPKKGDSVFVSAACGAVGQLVGQLAK 186

Query: 183 LHGCYVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEM 242
           LHGCYVVGSAGS +KV +LK +LG+DEAFNYK+E DL + L+RYFP+GID+YFDNVGG M
Sbjct: 187 LHGCYVVGSAGSKQKVEILKNELGYDEAFNYKEEADLDTALKRYFPEGIDIYFDNVGGSM 246

Query: 243 LEAAVANLKAFGRVALCGVIS--EYTSSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDF 302
           L+AA+ N+K  GR+ALCG++S    ++S +    +   +YKR+++EGFL  D+L+++  F
Sbjct: 247 LDAALLNMKVRGRIALCGMVSLQSLSTSSQGIKNLYSAIYKRLRLEGFLQSDYLHIFPQF 306

Query: 303 IAMASQYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
           +    +Y   GKI  +ED+S G+D  P+A +GLF G NIGK++V++A
Sbjct: 307 LENVKRYYKEGKIVYVEDISEGLDLAPAALVGLFSGKNIGKQVVRVA 348

BLAST of Cp4.1LG04g09400 vs. TAIR 10
Match: AT3G03080.1 (Zinc-binding dehydrogenase family protein )

HSP 1 Score: 282.0 bits (720), Expect = 6.1e-76
Identity = 144/342 (42.11%), Postives = 212/342 (61.99%), Query Frame = 0

Query: 3   VMNRVVAIRHHIDEFPVESDFELKSEALIVSVKPGCDDIVVKNLYVSIDPYQINRMKSHS 62
           V N+ V +R ++  FP ESD       + + +  G   ++VKNLY+S DPY   R++   
Sbjct: 9   VANKQVILRDYVTGFPKESDLIFTDSTIDLKIPEGSKTVLVKNLYLSCDPYM--RIRMGK 68

Query: 63  SSQKTSSFATSIKPAQPIDAYGVGEVLASGNADFTKGDLVAGLLHWAD------------ 122
               T++ A    P +PI  + V +V+ SG+ D+ KGDL+ GL+ W +            
Sbjct: 69  PDPGTAALAPHYIPGEPIYGFSVSKVIDSGHPDYKKGDLLWGLVGWGEYSLITPDFSHYK 128

Query: 123 ------------------GLTAYAGLFEVAKVKEGETVFVSAASGSVGSLAGQLAKLHGC 182
                             G+TAYAG +E+   K+GETVFVSAASG+VG L GQ AK+ GC
Sbjct: 129 IQHTDVPLSYYTGLLGMPGMTAYAGFYEICSPKKGETVFVSAASGAVGQLVGQFAKIMGC 188

Query: 183 YVVGSAGSDEKVALLKEKLGFDEAFNYKQEKDLTSTLERYFPDGIDVYFDNVGGEMLEAA 242
           YVVGSAGS+EKV LLK K GFD+AFNYK E DL + L+R FP+GID+YF+NVGG+ML+A 
Sbjct: 189 YVVGSAGSNEKVDLLKNKFGFDDAFNYKAEPDLNAALKRCFPEGIDIYFENVGGKMLDAV 248

Query: 243 VANLKAFGRVALCGVISEYT-SSKKAAPKMLDLVYKRIKVEGFLAGDFLNVYSDFIAMAS 302
           + N+K  GR+A+CG+IS+Y    ++    + +++YKRI+++GF+  D+ + +  F+    
Sbjct: 249 LLNMKLHGRIAVCGMISQYNLEDQEGVHNLANVIYKRIRIKGFVVSDYFDKHLKFLDFVL 308

Query: 303 QYLGSGKIESLEDVSIGVDTIPSAFIGLFKGDNIGKKMVKLA 314
            Y+  GKI  +EDV  G++  PSA +GLF G N+GK+++ +A
Sbjct: 309 PYIREGKITYVEDVVEGLENGPSALLGLFHGKNVGKQLIAVA 348