Cp4.1LG04g08660 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g08660 vs. NCBI nr
Match: XP_023539563.1 (uncharacterized protein LOC111800203 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216 bits (551), Expect = 1.37e-60
Identity = 120/226 (53.10%), Postives = 136/226 (60.18%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG--------- 120
           ++ + K IKKCVNLKHLDL  CN LTH+ KGLGELTSLQT NLF+LKKDIG         
Sbjct: 685 LRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCDLSELNRL 744

Query: 121 -----------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVL 180
                             DL  SSFN + +LGVQEL  V NYS+DEHMN I+D EC GVL
Sbjct: 745 NKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTIVDNECNGVL 804

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 227
            CLQPHSKVQKIDI +Y  V                           LPQ DQF FLK+L
Sbjct: 805 HCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKYL 864

BLAST of Cp4.1LG04g08660 vs. NCBI nr
Match: XP_023547024.1 (disease resistance protein RGA2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023547025.1 disease resistance protein RGA2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210 bits (535), Expect = 6.25e-59
Identity = 132/283 (46.64%), Postives = 156/283 (55.12%), Query Frame = 0

Query: 22  DDFETHYNIQKDALIKQWVAQAYCCIAQFKNT-YLSRLFS---------------IKRIA 81
           DD +    + +DA  K W+   Y  +A+ K   YL    +               +K + 
Sbjct: 607 DDCKLLQELPRDA--KNWMKLRYLSLARNKEIKYLPDSIAALHNLETLILQDCSLLKELP 666

Query: 82  KYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG-------------- 141
           K IKKCVNLKHLDL  C+ LTH+PKGLGELT LQT NLF+LKKDIG              
Sbjct: 667 KDIKKCVNLKHLDLRGCHALTHMPKGLGELTRLQTMNLFVLKKDIGCDLSELNRLDKLKG 726

Query: 142 ------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVLKCLQP 201
                        DL  SSFN +L  GVQELK V N S+DEHMNI+LD E KGVL+CLQP
Sbjct: 727 SLTIKGLEVCTTDDLEKSSFNLRLMSGVQELKLVWNNSKDEHMNIVLDNEGKGVLQCLQP 786

Query: 202 HSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHLSLKSI 228
           HSKV+ IDI  YR+V                           LPQ DQF FLKHLSLKS+
Sbjct: 787 HSKVRNIDICGYREVRLCDWLSSNTLIHLVSIKLSDCFKLQALPQFDQFPFLKHLSLKSL 846

BLAST of Cp4.1LG04g08660 vs. NCBI nr
Match: XP_023547023.1 (disease resistance protein RGA2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 210 bits (535), Expect = 8.13e-59
Identity = 132/283 (46.64%), Postives = 156/283 (55.12%), Query Frame = 0

Query: 22  DDFETHYNIQKDALIKQWVAQAYCCIAQFKNT-YLSRLFS---------------IKRIA 81
           DD +    + +DA  K W+   Y  +A+ K   YL    +               +K + 
Sbjct: 648 DDCKLLQELPRDA--KNWMKLRYLSLARNKEIKYLPDSIAALHNLETLILQDCSLLKELP 707

Query: 82  KYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG-------------- 141
           K IKKCVNLKHLDL  C+ LTH+PKGLGELT LQT NLF+LKKDIG              
Sbjct: 708 KDIKKCVNLKHLDLRGCHALTHMPKGLGELTRLQTMNLFVLKKDIGCDLSELNRLDKLKG 767

Query: 142 ------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVLKCLQP 201
                        DL  SSFN +L  GVQELK V N S+DEHMNI+LD E KGVL+CLQP
Sbjct: 768 SLTIKGLEVCTTDDLEKSSFNLRLMSGVQELKLVWNNSKDEHMNIVLDNEGKGVLQCLQP 827

Query: 202 HSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHLSLKSI 228
           HSKV+ IDI  YR+V                           LPQ DQF FLKHLSLKS+
Sbjct: 828 HSKVRNIDICGYREVRLCDWLSSNTLIHLVSIKLSDCFKLQALPQFDQFPFLKHLSLKSL 887

BLAST of Cp4.1LG04g08660 vs. NCBI nr
Match: XP_022931674.1 (putative disease resistance protein RGA1 [Cucurbita moschata] >XP_022931675.1 putative disease resistance protein RGA1 [Cucurbita moschata])

HSP 1 Score: 209 bits (531), Expect = 5.47e-58
Identity = 118/227 (51.98%), Postives = 135/227 (59.47%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG--------- 120
           ++ + K IKKCVNLKHLDL  CN LTH+PKGLGELT+LQT NLFIL KD+G         
Sbjct: 690 LRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLFILNKDVGCDLSELNRL 749

Query: 121 -----------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVL 180
                             DL NS FN +L+LG QELKFV NYS+DEHMNI+LD ECKGVL
Sbjct: 750 SKLRGSLTIKGLEVCTTEDLKNSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKGVL 809

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 228
            CLQPHS V KIDI  Y +V                           LPQ DQF  LK L
Sbjct: 810 NCLQPHSNVGKIDICGYPEVMLCDWLSSNTLLHLVSIKLSDCFQLQVLPQFDQFPCLKCL 869

BLAST of Cp4.1LG04g08660 vs. NCBI nr
Match: XP_023528691.1 (putative disease resistance protein RGA1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 205 bits (522), Expect = 4.33e-57
Identity = 115/227 (50.66%), Postives = 133/227 (58.59%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG--------- 120
           ++ + K IKKCVNLKHLDL  CN LTH+PKG+GELT LQT NLF+LKKDIG         
Sbjct: 459 LRELPKDIKKCVNLKHLDLHGCNALTHLPKGIGELTRLQTMNLFVLKKDIGCDLSELNRL 518

Query: 121 -----------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVL 180
                             DL  SS N  L+LGVQ+LK V  YSED+HMN +LD ECK VL
Sbjct: 519 NNLKGSLTVKGLEACTTDDLEKSSLNLGLKLGVQKLKLVWTYSEDQHMNNVLDNECKEVL 578

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 228
           KCLQPHS V++I I  YR+V                           LPQ +QF FLKHL
Sbjct: 579 KCLQPHSNVRRIHICGYREVRLCDWLSSNTLFNLVSIKLSDCFKLQALPQFNQFPFLKHL 638

BLAST of Cp4.1LG04g08660 vs. ExPASy TrEMBL
Match: A0A6J1EUW9 (putative disease resistance protein RGA1 OS=Cucurbita moschata OX=3662 GN=LOC111437812 PE=4 SV=1)

HSP 1 Score: 209 bits (531), Expect = 2.65e-58
Identity = 118/227 (51.98%), Postives = 135/227 (59.47%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIG--------- 120
           ++ + K IKKCVNLKHLDL  CN LTH+PKGLGELT+LQT NLFIL KD+G         
Sbjct: 690 LRELPKDIKKCVNLKHLDLRGCNALTHLPKGLGELTNLQTMNLFILNKDVGCDLSELNRL 749

Query: 121 -----------------HDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVL 180
                             DL NS FN +L+LG QELKFV NYS+DEHMNI+LD ECKGVL
Sbjct: 750 SKLRGSLTIKGLEVCTTEDLKNSYFNLRLKLGFQELKFVWNYSKDEHMNIVLDNECKGVL 809

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 228
            CLQPHS V KIDI  Y +V                           LPQ DQF  LK L
Sbjct: 810 NCLQPHSNVGKIDICGYPEVMLCDWLSSNTLLHLVSIKLSDCFQLQVLPQFDQFPCLKCL 869

BLAST of Cp4.1LG04g08660 vs. ExPASy TrEMBL
Match: A0A6J1FNX2 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111445964 PE=4 SV=1)

HSP 1 Score: 196 bits (499), Expect = 5.92e-54
Identity = 111/223 (49.78%), Postives = 135/223 (60.54%), Query Frame = 0

Query: 61   IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIGHDL------ 120
            ++ + K IKKCVNLKHL+L  C  LTH+PKGLGELTSLQT NLF+LKKDIG DL      
Sbjct: 834  LRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELNRL 893

Query: 121  --VNSSFNFQ---------------LRLGVQELKFVRNYSEDEHMNIILDKECKGVLKCL 180
              +  S   +               L+LGVQEL+ V NYSED+ MNI+LD ECKG  K L
Sbjct: 894  DKLEGSLTIEGLEVCTFDVLEKSLPLKLGVQELELVWNYSEDQDMNIVLDNECKGGFKWL 953

Query: 181  QPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHLSLK 227
            QPHS ++ IDI  Y +V                           LPQ DQF FLK+L+L+
Sbjct: 954  QPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQFPFLKYLTLQ 1013

BLAST of Cp4.1LG04g08660 vs. ExPASy TrEMBL
Match: A0A0A0K5Z6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G425920 PE=4 SV=1)

HSP 1 Score: 129 bits (325), Expect = 1.10e-30
Identity = 86/225 (38.22%), Postives = 110/225 (48.89%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIGHDLVNSSFN 120
           ++ +   I   +NLKHLD+  C  LTH+PKGLG LTSLQT NLF+L KD G DL   +  
Sbjct: 239 LRELPTDINNLINLKHLDVHGCYRLTHMPKGLGGLTSLQTMNLFVLGKDKGCDLSELNEL 298

Query: 121 FQLR-------------------------LGVQELKFVRNYS-EDEHMNIILDKECKGVL 180
            +LR                          G+Q+LK   N    D   +   + + + VL
Sbjct: 299 ARLRGSLLIKGLELCTTTDLKNAKYMEEKFGIQKLKLRWNRDLYDAETDYASENDDERVL 358

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 226
            CL+PHS V K+ I  YR V                           LPQ DQF FLKHL
Sbjct: 359 DCLKPHSNVHKMQIRGYRGVKLCNWLSFDYLGGLVNIELQSCEKLQHLPQFDQFPFLKHL 418

BLAST of Cp4.1LG04g08660 vs. ExPASy TrEMBL
Match: A0A6J1DMC1 (putative disease resistance protein RGA3 OS=Momordica charantia OX=3673 GN=LOC111021883 PE=4 SV=1)

HSP 1 Score: 125 bits (313), Expect = 4.56e-29
Identity = 80/221 (36.20%), Postives = 110/221 (49.77%), Query Frame = 0

Query: 69  KKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILK--------------------- 128
           K  ++L+HLD+CDC  LTH+PKGLGE+TSLQT NLF+L                      
Sbjct: 639 KNLISLRHLDMCDCYALTHMPKGLGEMTSLQTMNLFVLGDTNSNGLSELNGLGNLKGSLT 698

Query: 129 ------KDIGHDLVNSSFNFQLRLGVQELKFVRNYSEDEHMNIILDKECKGVLKCLQPHS 188
                 + IG DL  +++  +L+ G++ L         +   I+   + + VL+CL+PH 
Sbjct: 699 IRDLQFQTIG-DLQKNAYYLKLKSGIKRLMLHWKDGRGKKSKIVALDDEERVLECLEPHP 758

Query: 189 KVQKIDIYQYR---------------------------KVLPQSDQFTFLKHLSLKSIPN 227
            +QKI+I  Y                            + LPQ DQ+ FLKHL L+S+  
Sbjct: 759 NLQKIEILGYHGARLCNWLSSDSLVRLVTIKLSHCSRLQQLPQFDQYPFLKHLCLESLIC 818

BLAST of Cp4.1LG04g08660 vs. ExPASy TrEMBL
Match: A0A0A0K9P7 (Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G425940 PE=4 SV=1)

HSP 1 Score: 120 bits (301), Expect = 1.88e-27
Identity = 83/227 (36.56%), Postives = 119/227 (52.42%), Query Frame = 0

Query: 61  IKRIAKYIKKCVNLKHLDLCDCNVLTHIPKGLGELTSLQTTNLFILKKDIGHDL--VNSS 120
           ++++ K I   +NL+HLD+ DC+ LTH+PKGLG +TSLQT ++F+L K+ G DL  +N  
Sbjct: 453 LRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGL 512

Query: 121 FNFQLRLGVQELKF-----VRNYSE----------DEHMNIILDKEC---------KGVL 180
            + +  L ++ L+F     ++N S           + H +I +D E          +GVL
Sbjct: 513 KSLRGLLCIKGLQFCTTADLKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVL 572

Query: 181 KCLQPHSKVQKIDIYQYRKV---------------------------LPQSDQFTFLKHL 227
           + L+PHS ++K+ I  YR +                           LPQ DQF +LKHL
Sbjct: 573 EGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHL 632