Cp4.1LG04g04760 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g04760 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 6.9e-226
Identity = 456/895 (50.95%), Postives = 590/895 (65.92%), Query Frame = 0

Query: 59  EPSGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDG-IGTGGSASWASG 118
           + +   EVI+K+D    +    S E+    GK+WR+  YDFW++ +G +  G +A+    
Sbjct: 4   DAADRREVIVKIDGENGNNNGVSGETV---GKIWRDGSYDFWTDGEGNLNKGHNAAAVDS 63

Query: 119 AR-ASDSGD--RNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLD 178
            R A+ +G+  ++EGFEF RRG   +DPPTKLIG FLHKQ+  GE  LD+DL M+EL+  
Sbjct: 64  DRSAATTGEQQKDEGFEF-RRG---EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ-S 123

Query: 179 RTKTPMMDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSH 238
           R  TP+ +SP        +VS + D         V RR                      
Sbjct: 124 RGLTPVSESP--------RVSTKRD--------PVGRR---------------------- 183

Query: 239 HEHGGSSISGVQNDSVAEAMR-SASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPA----- 298
                 S S   N+   E ++ S +N   Q +      S LLK + +SRL+DPP      
Sbjct: 184 -----DSRSNTNNNDDGEVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQLPP 243

Query: 299 -EPDRLSGLVNKSGQLRSGFLGRT-----DDEEDDPFLEEDFPDDFRRGKLDALTVLQWI 358
              D  SG + KSGQ++SGF G++     ++EEDDPF  ED P+++R+ KL    VL+W+
Sbjct: 244 QTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWL 303

Query: 359 SLFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNF 418
           SL LI A  VCTL+I  LR+  LWE  +WKWE MVLVLICGRLVS W + I VFFIERNF
Sbjct: 304 SLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNF 363

Query: 419 LLRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLS 478
           LLRK+VLYFVYG+++ VQNCLWLGLVL+AWH LFD++V    N+  L  V K  VCLL+ 
Sbjct: 364 LLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVG 423

Query: 479 TLIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIAD 538
            L+WL KTL++KVLASSFH+STYFDRIQESLF QYVIETLSGPP IE+QK+EEE+++I+ 
Sbjct: 424 FLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISV 483

Query: 539 EVQRLQSAGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNG----- 598
           EV++ Q+ G                 G  ++S         GK P LS V +  G     
Sbjct: 484 EVKKFQNPG-----------------GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGE 543

Query: 599 SEGINIDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSERE 658
           ++GI ID LHKL  KNVSAW MKRL+ I+R GS+TTLDEQ+Q PSLDD+    +I+SE E
Sbjct: 544 NKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDK-GNQIRSEFE 603

Query: 659 AKAAAKKIFQNVARPG---------------------FNRFEGATETRRISKSALKNWVV 718
           AK AA+KIF NVA+PG                      + FEGA+ET RISKS+LKNWVV
Sbjct: 604 AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVV 663

Query: 719 NAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVL 778
           NAFRERRAL+LTLNDTKTAV RL  +VN++  I I+V+W+I+LGI S K ++ +SSQ+V+
Sbjct: 664 NAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVV 723

Query: 779 VAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIV 838
           VAFIFGN  K +FE+II+LFV+HPFDVGDRCEID +QMVVEEMNILTTVFLR DN K++ 
Sbjct: 724 VAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVY 783

Query: 839 PNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPII 898
           PNS+L TK I N+YRSPDMG+ IE  +HI TP EKI  +KQRI SYIE  K+HW P+P+I
Sbjct: 784 PNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMI 824

Query: 899 LMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 912
           + KD++S + + +++W +H MNHQD+ E+WARRS +VEE+ KIC+E DI   L P
Sbjct: 844 VFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYP 824

BLAST of Cp4.1LG04g04760 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 780.8 bits (2015), Expect = 1.7e-224
Identity = 471/950 (49.58%), Postives = 621/950 (65.37%), Query Frame = 0

Query: 4   FKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEPSGH 63
           F+ S K +  +K   +I + G  SE + E LPIL DH P                    H
Sbjct: 3   FRNSFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHHPD-------------------H 62

Query: 64  NEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARASD- 123
           + ++  VDD    + +RS    G    V R++ Y FW +N    T G+++  +  R SD 
Sbjct: 63  SGMV--VDDQKPDS-TRSSLDDGRNAPVERDASYKFWQDN----TTGTSTDHTAVRTSDK 122

Query: 124 -------SGDRNEG-FEFVRRGNGTDDPPTKLIGDFLHKQKVRG----ETTLDLDLEMEE 183
                   GDR  G F+FV      D+ PTK++      ++ RG    E TLD+D E ++
Sbjct: 123 DPIAISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDD 182

Query: 184 LRLDRTKTPMMDSPLS-RASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPS 243
           +      TP   +  S  AS++++VSF           +VRR    F         G   
Sbjct: 183 VSHQTMPTPTSTARTSFDASREMRVSF-----------NVRRAGGAF-------VAGSVP 242

Query: 244 WHQSHHEHGGSSISGVQND----SVAEAMRSASNLSFQSTLSLHRKSHLL-KAKGKSRLT 303
              SH     S+      D       E +R  SN+SFQ      RKS L+ + K +SRL 
Sbjct: 243 SSSSHSSSSSSATMRTNQDQPQLQEEEVVRCTSNMSFQ------RKSELISRVKTRSRLQ 302

Query: 304 DPPAEPDR-LSGLVNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWIS 363
           DPP E +   SG   +SGQL+SG L    DEEDDP  EED PD+++RGKLDA+T+LQW+S
Sbjct: 303 DPPREEETPYSGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLDAITLLQWLS 362

Query: 364 LFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFL 423
           L  I AAL C+LSI+  ++  +W   +WKWEV +LVLICGRLVSGWGI I VFFIERNFL
Sbjct: 363 LVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFL 422

Query: 424 LRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLST 483
           LRK+VLYFVYG++R VQNCLWLGLVL+AWH LFDK+V+ +T S  L YV K LVC LLST
Sbjct: 423 LRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLST 482

Query: 484 LIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADE 543
           ++WL KTL++KVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP IE+ + EEE+++  DE
Sbjct: 483 ILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDE 542

Query: 544 VQRLQSAGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSE-GIN 603
           + ++Q+AG  +PPDL AAA    KSG             R  +PKLS +  K+ ++ GI+
Sbjct: 543 IFKMQNAGANLPPDLCAAAFPPGKSG-------------RVMNPKLSPIIPKSTTDNGIS 602

Query: 604 IDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAA 663
           ++HLH++  KN+SAWNMKRL+KIVR  S+TTLDEQ+   + +DES T +I+SE+EAKAAA
Sbjct: 603 MEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDES-TRQIRSEKEAKAAA 662

Query: 664 KKIFQNVARPG---------------------FNRFEGATETRRISKSALKNWVVNAFRE 723
           +KIF+NV + G                        FEGA E +RISKSALKNW+VNAFRE
Sbjct: 663 RKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRE 722

Query: 724 RRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIF 783
           RRAL+LTLNDTKTAV +L  ++N++ +I I+V+W++LL IAS KV+L++SSQ+VL+AFIF
Sbjct: 723 RRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIF 782

Query: 784 GNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVL 843
           GNT+K +FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLR+DNLKI+ PNS+L
Sbjct: 783 GNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLL 842

Query: 844 ATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDI 903
             K I+N+YRSPDMG+ IE CVHI TPLEKI+ +KQRI +YI++  E+W P   I++KD+
Sbjct: 843 WQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDL 882

Query: 904 DSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 912
           +    + ++IW  H +NHQD+ ERW RR+V+VEEV+KI  E DI     P
Sbjct: 903 EDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYP 882

BLAST of Cp4.1LG04g04760 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 1.3e-213
Identity = 429/907 (47.30%), Postives = 585/907 (64.50%), Query Frame = 0

Query: 59  EPSGHNEVILKVDDGGSSAVSRSQESA-GYGGKVWRESRYDFWSNNDGIGTGGSASWASG 118
           + +   + I+ ++   S AV  +  S+   GG +W+ES YDFW    G          + 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKG---------KND 64

Query: 119 ARASDSGDRNEGFEFVRRGN------GTDDPPTKLIGDFLHKQKVRG-ETTLDLDLEMEE 178
            +  D  +    F F +RG          DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 179 LRLDRTKTPMMDSPLSRASKDLKVSFQHDSTEISSN---ESVRRRYKEFREMQEESKRGQ 238
           L   ++ TP   +  S   +    +    S+ + +    ++VRRR               
Sbjct: 125 L---QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------------- 184

Query: 239 PSWHQSHHEHGGSSISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPP 298
               Q+    GGSS    +N   AE ++  S           +K  L + K KSRL DPP
Sbjct: 185 ----QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPP 244

Query: 299 A-------EPDRLSGLVNKSGQLRSGFLGRT------------DDEEDDPFLEEDFPDDF 358
                   + +  SG   +SG  +SGFLG++            ++EE+DPFL+ED P++F
Sbjct: 245 TPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEF 304

Query: 359 RRGKLDALTVLQWISLFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSG 418
           +R KL     L+WISL LI  +LVC+L+I +L+  + W+  +WKWEV VLVLICGRLVS 
Sbjct: 305 KRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSS 364

Query: 419 WGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDI 478
           W + I VF +E+NF  RK+VLYFVYG+++ VQNCLWLGLVL+AWH LFDK+V+ +T S  
Sbjct: 365 WIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTA 424

Query: 479 LDYVRKSLVCLLLSTLIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRI 538
           L YV + LVCLL++ +IWL KT+++KVLASSFH+STYFDRIQESLF QYVIETLSGPP +
Sbjct: 425 LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLM 484

Query: 539 ELQKSEEEQKKIADEVQRLQS-AGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDP 598
           E+Q+ EEE++++A++V+ L+  AG  +PP L+A   S +K                GK P
Sbjct: 485 EIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKV---------------GKSP 544

Query: 599 KLSRVSTKNG--SEGINIDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDD 658
            L+R+ +K G   EGI ID L ++ +KNVSAWNMKRL+ I+  G+I+TLD+ +Q  + +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 659 ESTTTEIKSEREAKAAAKKIFQNVARPG--------FNR-------------FEGATETR 718
           E   T I+SE EAK AA+KIF NV  PG        F R             FEGA+E+ 
Sbjct: 605 ED-ATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESD 664

Query: 719 RISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASR 778
           +ISKS LKNWVV AFRERRAL+LTLNDTKTAV+RL  ++NV+  I II++W+++LGIA+ 
Sbjct: 665 KISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 724

Query: 779 KVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTT 838
           + +L +SSQ++LVAF+FGN+ K IFEAIIFLFVMHPFDVGDRCEID +Q+VVEEMNILTT
Sbjct: 725 RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTT 784

Query: 839 VFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIE 898
           VFLR+DN KII PNSVL TK I N+YRSPDMG+ +E CVHI TP EKITA+KQRI+SY++
Sbjct: 785 VFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVD 844

Query: 899 SDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFD 912
           + K++W P+P+I+   +D  + + +++WL+H MNHQD+ ER+ RR +++EEV K C+E D
Sbjct: 845 NKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELD 851

BLAST of Cp4.1LG04g04760 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 1.9e-207
Identity = 419/872 (48.05%), Postives = 568/872 (65.14%), Query Frame = 0

Query: 83  ESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARASDSGDRNEG-FEFVRRGNGTD- 142
           E  G   K WRES  +FW N+      G             G+ ++G F+F+RR +    
Sbjct: 19  EDNGDSEKFWRESSINFWHNDKSSKPPG-------------GEEDDGSFDFMRRSSEKSE 78

Query: 143 --DPPTKLIGDFLHKQKVRG-ETTLDLDLEMEELRLDRTKTPMMDSPLSRASKDLKVSFQ 202
             DPP+KLI  FL+KQK  G E +LD++  M EL+   T  P+  + +S ++  +     
Sbjct: 79  EPDPPSKLINQFLNKQKASGDEISLDMEANMPELQ-KNTVPPLSSTAVSGSASPVTAPVT 138

Query: 203 HDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGSSISGVQNDSVAEAMRSA 262
             S    + +++RRR      +    K G  S  + +   G            +E ++  
Sbjct: 139 -ASYRNGTGDAIRRRQNRV-TLSPSVKDGDSSEDEENRVDG------------SEVVKCT 198

Query: 263 SNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAE--PDRLSGLVNKSGQLRSGFLGRTD--- 322
           SN   +ST+   R   L+K K +SRL DPP    PD +SG   +SG L  GF GR     
Sbjct: 199 SN---RSTM---RTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPG 258

Query: 323 ----------DEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHLRE 382
                     +EE+DPF EED P+  R+ K+    +++WI L LI A+L+C+L I +LR 
Sbjct: 259 TPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRG 318

Query: 383 NSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQNC 442
            +LW+ ++WKWEVMVLVLICGRLVS W + + V+F+E NFL RKKVLYFVYGI++PVQNC
Sbjct: 319 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 378

Query: 443 LWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSFHV 502
           LWLGLVLIAWH LFDK+V+ +  S +L YV K L+CLL++ +IWL KTL++KVLASSFH+
Sbjct: 379 LWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHM 438

Query: 503 STYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRAAA 562
           STYFDRIQESLF QYVIETLSGPPRIE+   EE   K+A++V+  +  G  + P L   A
Sbjct: 439 STYFDRIQESLFTQYVIETLSGPPRIEIHIEEE---KVANDVKTFEIVGRKLSP-LGPKA 498

Query: 563 LSSIKSGERVRSNVASQRSLRGKDPKLSRVSTK--NGSEGINIDHLHKLTSKNVSAWNMK 622
           +SS          +    S  GK P LSR  +K   G EGI IDHL ++ +KNVSAW MK
Sbjct: 499 VSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMK 558

Query: 623 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPG------- 682
           +L+ +++ G+++TLDEQIQ  +  ++   T+I+SE EAK AA+KIFQNVA PG       
Sbjct: 559 KLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYME 618

Query: 683 --------------FNRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 742
                          + FEGA+E  +ISKS LKNWVVNAFRERRAL+LTLNDTKTAV RL
Sbjct: 619 DFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRL 678

Query: 743 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 802
             +V+V+ SI I+++W+++LGIA+ K +L ISSQ++LV F+FGN+ K IFEA+IF+FVMH
Sbjct: 679 HRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMH 738

Query: 803 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 862
           PFDVGDRCEID +QM+VEEMNILTTVFLR DN KI+ PNS+L TK I N+YRSPDM + I
Sbjct: 739 PFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAI 798

Query: 863 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 912
           E  VHI TP EK TA++QRI+SY+++ K+HW PSP+I+ +D+   + + +++W +H MNH
Sbjct: 799 EFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNH 852

BLAST of Cp4.1LG04g04760 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 1.8e-194
Identity = 391/763 (51.25%), Postives = 520/763 (68.15%), Query Frame = 0

Query: 174 DRTKTPMMDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQS 233
           D TK+   D P  R    L V+ + + TE  SN +     KE R   + +  G  +   S
Sbjct: 65  DYTKSGSFDFPQYREEITLDVNEETEETEDVSNNNNLSGSKETRVFFKINSSGTNNMSGS 124

Query: 234 HHEHGGSSISGVQNDSVAEAMRSASNLSFQSTL----SLHRKSHLL-KAKGKSRLTDPPA 293
                 S  S     S    +     L  +  +    S  RK+ L+ +AK +SRL DPP 
Sbjct: 125 VR----SCTSSTSFSSATMRLNLEQQLEDEGEVVVRCSSVRKTELVSRAKARSRLIDPPQ 184

Query: 294 EPD-RLSGLVNKSGQLRSGFLGRTDD---EEDDPFLEEDFPDDFRRGKLDALTVLQWISL 353
           E + + S  +  S QLRSG LGR  D   EEDD   EED P ++R+ K+DA+T+LQW+SL
Sbjct: 185 EEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSL 244

Query: 354 FLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLL 413
             +  ALV +L +   R  +LW   +WKWEV++LVLICGRLVSG GI I VFFIERNFLL
Sbjct: 245 IALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLL 304

Query: 414 RKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTL 473
           RK+VLYFVYG+K  VQNCLWLGLVL+AWH LFDK+V+ +T SD+L  + K LVC LLST+
Sbjct: 305 RKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTV 364

Query: 474 IWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEV 533
           +WL KTL++KVLASSFHVSTYFDRIQE+LF+ Y+IETLSGPP +EL + EEE+ +  DE+
Sbjct: 365 LWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEI 424

Query: 534 QRLQSAGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSE-GINI 593
            ++Q  G  + P+L +AA    KSG  +             + K S +  K GS+ GI +
Sbjct: 425 YKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKFSPIIPKTGSDNGITM 484

Query: 594 DHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAK 653
           D LHK+  KNVSAWNMKRL+KIVR  S++TLDEQ    + +DES T +I+SE+EAKAAA+
Sbjct: 485 DDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDES-TRQIRSEKEAKAAAR 544

Query: 654 KIFQNVARPGFNR---------------------FEGATETRRISKSALKNWVVNAFRER 713
           KIF+NVA+PG                        FEGA  T++I+KSALKNW+VNAFRER
Sbjct: 545 KIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRER 604

Query: 714 RALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFG 773
           RAL+LTLNDTKTAV +L  +++ + +I IIV+W+ILL IA+ K +L+++SQ+VL+AF+FG
Sbjct: 605 RALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFG 664

Query: 774 NTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLA 833
           N+LK +FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTTVFLR DNLKI+ PN +L 
Sbjct: 665 NSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLW 724

Query: 834 TKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDID 893
            K IHN+ RSPDMG+ +  CVHI TP EKI A+KQRI SYI+S  E+W P   +++KD++
Sbjct: 725 QKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVE 784

Query: 894 SADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDI 906
             + + ++IWL H +NHQ++ ER+ RR++++EEV+KI  E DI
Sbjct: 785 DLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDI 809

BLAST of Cp4.1LG04g04760 vs. NCBI nr
Match: XP_023529593.1 (mechanosensitive ion channel protein 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1748 bits (4526), Expect = 0.0
Identity = 912/934 (97.64%), Postives = 913/934 (97.75%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP
Sbjct: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
           GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900
           EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

Query: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 913
           QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP
Sbjct: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 934

BLAST of Cp4.1LG04g04760 vs. NCBI nr
Match: XP_022928129.1 (mechanosensitive ion channel protein 6-like [Cucurbita moschata])

HSP 1 Score: 1730 bits (4481), Expect = 0.0
Identity = 902/934 (96.57%), Postives = 909/934 (97.32%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLK NGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH+EVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLMLKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
           GLVVNVIFSIFI+VLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 GLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900
           EICVHIMTPLEKITAMKQRIIS+IE+DKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 EICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

Query: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 913
           QDIRERWARRSVVVEEVLKICQEFDITP+LVPPP
Sbjct: 901 QDIRERWARRSVVVEEVLKICQEFDITPTLVPPP 934

BLAST of Cp4.1LG04g04760 vs. NCBI nr
Match: XP_022989307.1 (mechanosensitive ion channel protein 6-like [Cucurbita maxima])

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 891/934 (95.40%), Postives = 904/934 (96.79%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRLKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH++VILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSDVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFL KQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLLKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEH GS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHRGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLM+KVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMVKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREA+AAAKKIFQNVARP F      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREARAAAKKIFQNVARPRFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATE+RRISKSAL NWVVNAFRERRALSLTLNDTKTAV+RL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATESRRISKSALNNWVVNAFRERRALSLTLNDTKTAVDRL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
            LVVNVIFSIFI+VLWIILLGIASRKVILYISSQIVL AFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 RLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLAAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900
           +ICVHIMTPLEKITAMKQRIIS+IE+DKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 QICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

Query: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 913
           QDIRERWARRSVVVEEVLKICQEFDITP+LVPPP
Sbjct: 901 QDIRERWARRSVVVEEVLKICQEFDITPTLVPPP 934

BLAST of Cp4.1LG04g04760 vs. NCBI nr
Match: KAG6589025.1 (Serine/threonine-protein kinase ATR, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1698 bits (4397), Expect = 0.0
Identity = 885/916 (96.62%), Postives = 892/916 (97.38%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH+EVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLMLKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQ+AGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQNAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
           GLVVNVIFSIFI+VLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 GLVVNVIFSIFIVVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 895
           EICVHIMTPLEKITAMKQRIIS+IE+DKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 EICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

BLAST of Cp4.1LG04g04760 vs. NCBI nr
Match: KAG7022741.1 (Mechanosensitive ion channel protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1639 bits (4243), Expect = 0.0
Identity = 861/913 (94.30%), Postives = 866/913 (94.85%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH+EVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLMLKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQ+AGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQNAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFNRFEGA 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFNRFEGA
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFNRFEGA 660

Query: 661 TETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLG 720
           TETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFI+VLWIILLG
Sbjct: 661 TETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIVVLWIILLG 720

Query: 721 IASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMN 780
           IASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMN
Sbjct: 721 IASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMN 780

Query: 781 ILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRII 840
           ILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEI                   
Sbjct: 781 ILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEIY------------------ 840

Query: 841 SYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKIC 900
                                    KLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKIC
Sbjct: 841 -------------------------KLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKIC 870

Query: 901 QEFDITPSLVPPP 913
           QEFDITP+LVPPP
Sbjct: 901 QEFDITPTLVPPP 870

BLAST of Cp4.1LG04g04760 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1730 bits (4481), Expect = 0.0
Identity = 902/934 (96.57%), Postives = 909/934 (97.32%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLK NGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH+EVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLMLKVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
           GLVVNVIFSIFI+VLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 GLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900
           EICVHIMTPLEKITAMKQRIIS+IE+DKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 EICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

Query: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 913
           QDIRERWARRSVVVEEVLKICQEFDITP+LVPPP
Sbjct: 901 QDIRERWARRSVVVEEVLKICQEFDITPTLVPPP 934

BLAST of Cp4.1LG04g04760 vs. ExPASy TrEMBL
Match: A0A6J1JFG4 (mechanosensitive ion channel protein 6-like OS=Cucurbita maxima OX=3661 GN=LOC111486413 PE=4 SV=1)

HSP 1 Score: 1709 bits (4426), Expect = 0.0
Identity = 891/934 (95.40%), Postives = 904/934 (96.79%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNR KVRRPANDGEP
Sbjct: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRLKVRRPANDGEP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120
           SGH++VILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA
Sbjct: 61  SGHSDVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARA 120

Query: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTPM 180
           SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFL KQKVRGETTLDLDLEMEELRLDRTKTPM
Sbjct: 121 SDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLLKQKVRGETTLDLDLEMEELRLDRTKTPM 180

Query: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGS 240
           MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEH GS
Sbjct: 181 MDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHRGS 240

Query: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300
           SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS
Sbjct: 241 SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLVNKS 300

Query: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360
           GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL
Sbjct: 301 GQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHL 360

Query: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQ 420
           RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYG+KRPVQ
Sbjct: 361 RENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKRPVQ 420

Query: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSF 480
           NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLL TLIWLAKTLM+KVLASSF
Sbjct: 421 NCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMVKVLASSF 480

Query: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540
           HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA
Sbjct: 481 HVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRA 540

Query: 541 AALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600
           AALSSIKSGERVRSNV SQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK
Sbjct: 541 AALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAWNMK 600

Query: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF------ 660
           RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREA+AAAKKIFQNVARP F      
Sbjct: 601 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREARAAAKKIFQNVARPRFKYIYLD 660

Query: 661 ---------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 720
                          +RFEGATE+RRISKSAL NWVVNAFRERRALSLTLNDTKTAV+RL
Sbjct: 661 DLMRFMKEEEVLKTLSRFEGATESRRISKSALNNWVVNAFRERRALSLTLNDTKTAVDRL 720

Query: 721 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 780
            LVVNVIFSIFI+VLWIILLGIASRKVILYISSQIVL AFIFGNTLKNIFEAIIFLFVMH
Sbjct: 721 RLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLAAFIFGNTLKNIFEAIIFLFVMH 780

Query: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840
           PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI
Sbjct: 781 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 840

Query: 841 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900
           +ICVHIMTPLEKITAMKQRIIS+IE+DKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH
Sbjct: 841 QICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 900

Query: 901 QDIRERWARRSVVVEEVLKICQEFDITPSLVPPP 913
           QDIRERWARRSVVVEEVLKICQEFDITP+LVPPP
Sbjct: 901 QDIRERWARRSVVVEEVLKICQEFDITPTLVPPP 934

BLAST of Cp4.1LG04g04760 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1254 bits (3244), Expect = 0.0
Identity = 667/936 (71.26%), Postives = 770/936 (82.26%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MD+ KKS K N  FKH+RKISAGG  SEIN EELPILL+HQ +D H         ND +P
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHH-------LVNDSDP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQES-AGYGGKVWRESRYDFWSNNDGIGTGGSASWASGAR 120
           S   EVILK+DDGGSSAVSRS +S A  GGKVWRESRYDFW NND  G G SAS   GAR
Sbjct: 61  SDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFW-NNDETGIGESASRVRGAR 120

Query: 121 ASDSGDR-NEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKT 180
            SDSGD  NEGF+FV+ G G +DPPTKLIGDFLHKQK+RGETTLD+DLEMEEL+ +R   
Sbjct: 121 MSDSGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PMMDSPLSRASKDLKVSFQHDSTEISSNE-SVRRRYKEFREMQEESKRGQPSWHQSHHEH 240
           P+ +SPLS+ SKDLKVSFQ DSTEISSN+ S+RRRY++  +++EE K  QP W QSHHE 
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 GGS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGL 300
            GS +ISGVQN+S+AEAMR ASNLSF S LS  RKS+LL+AK KSRLTDPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 VNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLS 360
           + KSGQLRSGFLG+ +D++DDPFLE+D PDDF+RG   ALTVLQW SL LITAA +CTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 IRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIK 420
           + +LRE SLWE  IWKWEVM+L+LICGRLVSGWGI I VFFIERNFLLRK+VLYFVYG++
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 RPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVL 480
           +PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+STLIWL KTLM+KVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSA 600
           DL+A   +SIKSG  + S   + +S   K  K SR  TKNG++GI IDHLHKL+ KNVSA
Sbjct: 541 DLKA-TFASIKSGRAISSE-RTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF-- 660
           WNMKRLL IVRYGSI+TLDEQI+GP LDDESTT EIKSEREAKAAAKKIFQNVAR G+  
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT-EIKSEREAKAAAKKIFQNVARRGYKY 660

Query: 661 -------------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTA 720
                              + FEGA E RRISKS LKNWVVN FRERRAL+LTLNDTKTA
Sbjct: 661 IYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTA 720

Query: 721 VERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFL 780
           V++L  +VNVIF I I++LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFL
Sbjct: 721 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 780

Query: 781 FVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDM 840
           FVMHPFDVGDRCEID  QMVVEEMNILTTVFLR+DNLKII+PNSVLATK+IHN YRSPDM
Sbjct: 781 FVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDM 840

Query: 841 GEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSH 900
           GE +E  VHI TP EKITAMKQRIISYIE +KEHWCP+P+I+ KDID  +KL +++WLSH
Sbjct: 841 GESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSH 900

Query: 901 TMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 911
            MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 RMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLP 925

BLAST of Cp4.1LG04g04760 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1251 bits (3238), Expect = 0.0
Identity = 666/936 (71.15%), Postives = 769/936 (82.16%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MD+ KKS K N  FKH+RKISAGG  SEIN EELPILL+HQ +D H         ND +P
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDHH-------LVNDSDP 60

Query: 61  SGHNEVILKVDDGGSSAVSRSQES-AGYGGKVWRESRYDFWSNNDGIGTGGSASWASGAR 120
           S   EVILK+DD GSSAVSRS +S A  GGKVWRESRYDFW NND  G G SAS   GAR
Sbjct: 61  SDRTEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFW-NNDETGIGESASRVRGAR 120

Query: 121 ASDSGDR-NEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKT 180
            SDSGD  NEGF+FV+ G G +DPPTKLIGDFLHKQK+RGETTLD+DLEMEEL+ +R   
Sbjct: 121 MSDSGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PMMDSPLSRASKDLKVSFQHDSTEISSNE-SVRRRYKEFREMQEESKRGQPSWHQSHHEH 240
           P+ +SPLS+ SKDLKVSFQ DSTEISSN+ S+RRRY++  +++EE K  QP W QSHHE 
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 GGS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGL 300
            GS +ISGVQN+S+AEAMR ASNLSF S LS  RKS+LL+AK KSRLTDPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 VNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLS 360
           + KSGQLRSGFLG+ +D++DDPFLE+D PDDF+RG   ALTVLQW SL LITAA +CTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 IRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIK 420
           + +LRE SLWE  IWKWEVM+L+LICGRLVSGWGI I VFFIERNFLLRK+VLYFVYG++
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 RPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVL 480
           +PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+STLIWL KTLM+KVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSA 600
           DL+A   +SIKSG  + S   + +S   K  K SR  TKNG++GI IDHLHKL+ KNVSA
Sbjct: 541 DLKA-TFASIKSGRAISSE-RTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSA 600

Query: 601 WNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF-- 660
           WNMKRLL IVRYGSI+TLDEQI+GP LDDESTT EIKSEREAKAAAKKIFQNVAR G+  
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT-EIKSEREAKAAAKKIFQNVARRGYKY 660

Query: 661 -------------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTA 720
                              + FEGA E RRISKS LKNWVVN FRERRAL+LTLNDTKTA
Sbjct: 661 IYLDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTA 720

Query: 721 VERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFL 780
           V++L  +VNVIF I I++LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFL
Sbjct: 721 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 780

Query: 781 FVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDM 840
           FVMHPFDVGDRCEID  QMVVEEMNILTTVFLR+DNLKII+PNSVLATK+IHN YRSPDM
Sbjct: 781 FVMHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDM 840

Query: 841 GEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSH 900
           GE +E  VHI TP EKITAMKQRIISYIE +KEHWCP+P+I+ KDID  +KL +++WLSH
Sbjct: 841 GESVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSH 900

Query: 901 TMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 911
            MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 RMNHQDSAERWARRSVLVEEVVKVCQELDIQYRLLP 925

BLAST of Cp4.1LG04g04760 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1232 bits (3187), Expect = 0.0
Identity = 656/936 (70.09%), Postives = 767/936 (81.94%), Query Frame = 0

Query: 1   MDSFKKSLKPNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRHKVRRPANDGEP 60
           MD+ KKS K N  FKH+RKISAGGAGSEIN EELPILL+HQ +D     + R P ND + 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SGHNEVILKVDDGGSS-AVSRSQESAGY-GGKVWRESRYDFWSNNDGIGTGGSASWASGA 120
           S   EVILK+D GGSS AVSRS +SAG  GG VWRESRYDFW NND IG G SA     +
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFW-NNDEIGIGESARMGDNS 120

Query: 121 RASDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKT 180
                 DRNEGFEFV+ G G +DPPTKLIGDFL KQK+ GETTLD+DLEMEEL+ +R   
Sbjct: 121 GV----DRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVP 180

Query: 181 PMMDSPLSRASKDLKVSFQHDSTEISSNE-SVRRRYKEFREMQEESKRGQPSWHQSHHEH 240
           P+ +SPLS+ SKDLKVSFQHDSTEISSN+ S+RRR ++  +++EESK GQ    Q HHE 
Sbjct: 181 PLAESPLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHER 240

Query: 241 GGS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGL 300
            GS +ISGVQN+S+AEAMR ASNLSF S LS  RKS+LL+AK KSRL DPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGL 300

Query: 301 VNKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLS 360
           + KSGQLRSGFLG+ +D++DDPFLE+D PD+F+RG   ALTVLQW+SL +ITAAL+CTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLS 360

Query: 361 IRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIK 420
           + +LRE SLWE  IWKWEVM+ +LICGRLVSGWGI IGVFFIERNFLLRK+VLYFVYG++
Sbjct: 361 VPYLREKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 RPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVL 480
           +PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+STLIWL KTLM+KVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPP 540

Query: 541 DLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSA 600
           DL+AA  +SIKSG  + S   + +S   K  KLSR  TKN ++GI IDHLHKL++KNVSA
Sbjct: 541 DLKAATFASIKSGREIGSG-RTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSA 600

Query: 601 WNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGF-- 660
           WNMKRLL IVRYGSI+TLDEQI+GP LDDESTT EIKSEREAKAAAKKIFQNVA  G+  
Sbjct: 601 WNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT-EIKSEREAKAAAKKIFQNVAHRGYKY 660

Query: 661 -------------------NRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTA 720
                              + FEGA E +RISKSALKNWVVNAFRERRAL+LTLNDTKTA
Sbjct: 661 IYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTA 720

Query: 721 VERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFL 780
           V++L  +VNVIF I I++LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFL
Sbjct: 721 VDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFL 780

Query: 781 FVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDM 840
           FVMHPFDVGDRCEID +QMVVEEMNILTTVFLR+DNLK+I+PNSVLATK+IHN YRSPDM
Sbjct: 781 FVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDM 840

Query: 841 GEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSH 900
           GE IE  VHI TP EKITAMK RIISYIE +KEHW P+P+I+ KDID  +K+ +++WLSH
Sbjct: 841 GESIEFLVHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSH 900

Query: 901 TMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 911
            MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 RMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 929

BLAST of Cp4.1LG04g04760 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 785.4 bits (2027), Expect = 4.9e-227
Identity = 456/895 (50.95%), Postives = 590/895 (65.92%), Query Frame = 0

Query: 59  EPSGHNEVILKVDDGGSSAVSRSQESAGYGGKVWRESRYDFWSNNDG-IGTGGSASWASG 118
           + +   EVI+K+D    +    S E+    GK+WR+  YDFW++ +G +  G +A+    
Sbjct: 4   DAADRREVIVKIDGENGNNNGVSGETV---GKIWRDGSYDFWTDGEGNLNKGHNAAAVDS 63

Query: 119 AR-ASDSGD--RNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLD 178
            R A+ +G+  ++EGFEF RRG   +DPPTKLIG FLHKQ+  GE  LD+DL M+EL+  
Sbjct: 64  DRSAATTGEQQKDEGFEF-RRG---EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ-S 123

Query: 179 RTKTPMMDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSH 238
           R  TP+ +SP        +VS + D         V RR                      
Sbjct: 124 RGLTPVSESP--------RVSTKRD--------PVGRR---------------------- 183

Query: 239 HEHGGSSISGVQNDSVAEAMR-SASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPA----- 298
                 S S   N+   E ++ S +N   Q +      S LLK + +SRL+DPP      
Sbjct: 184 -----DSRSNTNNNDDGEVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQLPP 243

Query: 299 -EPDRLSGLVNKSGQLRSGFLGRT-----DDEEDDPFLEEDFPDDFRRGKLDALTVLQWI 358
              D  SG + KSGQ++SGF G++     ++EEDDPF  ED P+++R+ KL    VL+W+
Sbjct: 244 QTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWL 303

Query: 359 SLFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNF 418
           SL LI A  VCTL+I  LR+  LWE  +WKWE MVLVLICGRLVS W + I VFFIERNF
Sbjct: 304 SLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNF 363

Query: 419 LLRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLS 478
           LLRK+VLYFVYG+++ VQNCLWLGLVL+AWH LFD++V    N+  L  V K  VCLL+ 
Sbjct: 364 LLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVG 423

Query: 479 TLIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIAD 538
            L+WL KTL++KVLASSFH+STYFDRIQESLF QYVIETLSGPP IE+QK+EEE+++I+ 
Sbjct: 424 FLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISV 483

Query: 539 EVQRLQSAGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNG----- 598
           EV++ Q+ G                 G  ++S         GK P LS V +  G     
Sbjct: 484 EVKKFQNPG-----------------GVEIQSGAQKSPMKTGKSPFLSHVLSNGGGGGGE 543

Query: 599 SEGINIDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSERE 658
           ++GI ID LHKL  KNVSAW MKRL+ I+R GS+TTLDEQ+Q PSLDD+    +I+SE E
Sbjct: 544 NKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDK-GNQIRSEFE 603

Query: 659 AKAAAKKIFQNVARPG---------------------FNRFEGATETRRISKSALKNWVV 718
           AK AA+KIF NVA+PG                      + FEGA+ET RISKS+LKNWVV
Sbjct: 604 AKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVV 663

Query: 719 NAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVL 778
           NAFRERRAL+LTLNDTKTAV RL  +VN++  I I+V+W+I+LGI S K ++ +SSQ+V+
Sbjct: 664 NAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVV 723

Query: 779 VAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIV 838
           VAFIFGN  K +FE+II+LFV+HPFDVGDRCEID +QMVVEEMNILTTVFLR DN K++ 
Sbjct: 724 VAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVY 783

Query: 839 PNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPII 898
           PNS+L TK I N+YRSPDMG+ IE  +HI TP EKI  +KQRI SYIE  K+HW P+P+I
Sbjct: 784 PNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMI 824

Query: 899 LMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDITPSLVP 912
           + KD++S + + +++W +H MNHQD+ E+WARRS +VEE+ KIC+E DI   L P
Sbjct: 844 VFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYP 824

BLAST of Cp4.1LG04g04760 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 744.6 bits (1921), Expect = 9.6e-215
Identity = 429/907 (47.30%), Postives = 585/907 (64.50%), Query Frame = 0

Query: 59  EPSGHNEVILKVDDGGSSAVSRSQESA-GYGGKVWRESRYDFWSNNDGIGTGGSASWASG 118
           + +   + I+ ++   S AV  +  S+   GG +W+ES YDFW    G          + 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKG---------KND 64

Query: 119 ARASDSGDRNEGFEFVRRGN------GTDDPPTKLIGDFLHKQKVRG-ETTLDLDLEMEE 178
            +  D  +    F F +RG          DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 179 LRLDRTKTPMMDSPLSRASKDLKVSFQHDSTEISSN---ESVRRRYKEFREMQEESKRGQ 238
           L   ++ TP   +  S   +    +    S+ + +    ++VRRR               
Sbjct: 125 L---QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------------- 184

Query: 239 PSWHQSHHEHGGSSISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPP 298
               Q+    GGSS    +N   AE ++  S           +K  L + K KSRL DPP
Sbjct: 185 ----QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPP 244

Query: 299 A-------EPDRLSGLVNKSGQLRSGFLGRT------------DDEEDDPFLEEDFPDDF 358
                   + +  SG   +SG  +SGFLG++            ++EE+DPFL+ED P++F
Sbjct: 245 TPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEF 304

Query: 359 RRGKLDALTVLQWISLFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSG 418
           +R KL     L+WISL LI  +LVC+L+I +L+  + W+  +WKWEV VLVLICGRLVS 
Sbjct: 305 KRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSS 364

Query: 419 WGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDI 478
           W + I VF +E+NF  RK+VLYFVYG+++ VQNCLWLGLVL+AWH LFDK+V+ +T S  
Sbjct: 365 WIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTA 424

Query: 479 LDYVRKSLVCLLLSTLIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRI 538
           L YV + LVCLL++ +IWL KT+++KVLASSFH+STYFDRIQESLF QYVIETLSGPP +
Sbjct: 425 LRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLM 484

Query: 539 ELQKSEEEQKKIADEVQRLQS-AGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDP 598
           E+Q+ EEE++++A++V+ L+  AG  +PP L+A   S +K                GK P
Sbjct: 485 EIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKV---------------GKSP 544

Query: 599 KLSRVSTKNG--SEGINIDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDD 658
            L+R+ +K G   EGI ID L ++ +KNVSAWNMKRL+ I+  G+I+TLD+ +Q  + +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 659 ESTTTEIKSEREAKAAAKKIFQNVARPG--------FNR-------------FEGATETR 718
           E   T I+SE EAK AA+KIF NV  PG        F R             FEGA+E+ 
Sbjct: 605 ED-ATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESD 664

Query: 719 RISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASR 778
           +ISKS LKNWVV AFRERRAL+LTLNDTKTAV+RL  ++NV+  I II++W+++LGIA+ 
Sbjct: 665 KISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 724

Query: 779 KVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTT 838
           + +L +SSQ++LVAF+FGN+ K IFEAIIFLFVMHPFDVGDRCEID +Q+VVEEMNILTT
Sbjct: 725 RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTT 784

Query: 839 VFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIE 898
           VFLR+DN KII PNSVL TK I N+YRSPDMG+ +E CVHI TP EKITA+KQRI+SY++
Sbjct: 785 VFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVD 844

Query: 899 SDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFD 912
           + K++W P+P+I+   +D  + + +++WL+H MNHQD+ ER+ RR +++EEV K C+E D
Sbjct: 845 NKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELD 851

BLAST of Cp4.1LG04g04760 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 724.2 bits (1868), Expect = 1.3e-208
Identity = 419/872 (48.05%), Postives = 568/872 (65.14%), Query Frame = 0

Query: 83  ESAGYGGKVWRESRYDFWSNNDGIGTGGSASWASGARASDSGDRNEG-FEFVRRGNGTD- 142
           E  G   K WRES  +FW N+      G             G+ ++G F+F+RR +    
Sbjct: 19  EDNGDSEKFWRESSINFWHNDKSSKPPG-------------GEEDDGSFDFMRRSSEKSE 78

Query: 143 --DPPTKLIGDFLHKQKVRG-ETTLDLDLEMEELRLDRTKTPMMDSPLSRASKDLKVSFQ 202
             DPP+KLI  FL+KQK  G E +LD++  M EL+   T  P+  + +S ++  +     
Sbjct: 79  EPDPPSKLINQFLNKQKASGDEISLDMEANMPELQ-KNTVPPLSSTAVSGSASPVTAPVT 138

Query: 203 HDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQSHHEHGGSSISGVQNDSVAEAMRSA 262
             S    + +++RRR      +    K G  S  + +   G            +E ++  
Sbjct: 139 -ASYRNGTGDAIRRRQNRV-TLSPSVKDGDSSEDEENRVDG------------SEVVKCT 198

Query: 263 SNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAE--PDRLSGLVNKSGQLRSGFLGRTD--- 322
           SN   +ST+   R   L+K K +SRL DPP    PD +SG   +SG L  GF GR     
Sbjct: 199 SN---RSTM---RTKTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGNLNPGFSGRNTKPG 258

Query: 323 ----------DEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSIRHLRE 382
                     +EE+DPF EED P+  R+ K+    +++WI L LI A+L+C+L I +LR 
Sbjct: 259 TPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYLRG 318

Query: 383 NSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQNC 442
            +LW+ ++WKWEVMVLVLICGRLVS W + + V+F+E NFL RKKVLYFVYGI++PVQNC
Sbjct: 319 KTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQNC 378

Query: 443 LWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTLIWLAKTLMLKVLASSFHV 502
           LWLGLVLIAWH LFDK+V+ +  S +L YV K L+CLL++ +IWL KTL++KVLASSFH+
Sbjct: 379 LWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLIKTLLVKVLASSFHM 438

Query: 503 STYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPDLRAAA 562
           STYFDRIQESLF QYVIETLSGPPRIE+   EE   K+A++V+  +  G  + P L   A
Sbjct: 439 STYFDRIQESLFTQYVIETLSGPPRIEIHIEEE---KVANDVKTFEIVGRKLSP-LGPKA 498

Query: 563 LSSIKSGERVRSNVASQRSLRGKDPKLSRVSTK--NGSEGINIDHLHKLTSKNVSAWNMK 622
           +SS          +    S  GK P LSR  +K   G EGI IDHL ++ +KNVSAW MK
Sbjct: 499 VSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMK 558

Query: 623 RLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPG------- 682
           +L+ +++ G+++TLDEQIQ  +  ++   T+I+SE EAK AA+KIFQNVA PG       
Sbjct: 559 KLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYME 618

Query: 683 --------------FNRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAVERL 742
                          + FEGA+E  +ISKS LKNWVVNAFRERRAL+LTLNDTKTAV RL
Sbjct: 619 DFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRL 678

Query: 743 GLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMH 802
             +V+V+ SI I+++W+++LGIA+ K +L ISSQ++LV F+FGN+ K IFEA+IF+FVMH
Sbjct: 679 HRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMH 738

Query: 803 PFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFI 862
           PFDVGDRCEID +QM+VEEMNILTTVFLR DN KI+ PNS+L TK I N+YRSPDM + I
Sbjct: 739 PFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRSPDMQDAI 798

Query: 863 EICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNH 912
           E  VHI TP EK TA++QRI+SY+++ K+HW PSP+I+ +D+   + + +++W +H MNH
Sbjct: 799 EFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMNH 852

BLAST of Cp4.1LG04g04760 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 681.0 bits (1756), Expect = 1.3e-195
Identity = 391/763 (51.25%), Postives = 520/763 (68.15%), Query Frame = 0

Query: 174 DRTKTPMMDSPLSRASKDLKVSFQHDSTEISSNESVRRRYKEFREMQEESKRGQPSWHQS 233
           D TK+   D P  R    L V+ + + TE  SN +     KE R   + +  G  +   S
Sbjct: 65  DYTKSGSFDFPQYREEITLDVNEETEETEDVSNNNNLSGSKETRVFFKINSSGTNNMSGS 124

Query: 234 HHEHGGSSISGVQNDSVAEAMRSASNLSFQSTL----SLHRKSHLL-KAKGKSRLTDPPA 293
                 S  S     S    +     L  +  +    S  RK+ L+ +AK +SRL DPP 
Sbjct: 125 VR----SCTSSTSFSSATMRLNLEQQLEDEGEVVVRCSSVRKTELVSRAKARSRLIDPPQ 184

Query: 294 EPD-RLSGLVNKSGQLRSGFLGRTDD---EEDDPFLEEDFPDDFRRGKLDALTVLQWISL 353
           E + + S  +  S QLRSG LGR  D   EEDD   EED P ++R+ K+DA+T+LQW+SL
Sbjct: 185 EEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSL 244

Query: 354 FLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLL 413
             +  ALV +L +   R  +LW   +WKWEV++LVLICGRLVSG GI I VFFIERNFLL
Sbjct: 245 IALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLL 304

Query: 414 RKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLSTL 473
           RK+VLYFVYG+K  VQNCLWLGLVL+AWH LFDK+V+ +T SD+L  + K LVC LLST+
Sbjct: 305 RKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTV 364

Query: 474 IWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEV 533
           +WL KTL++KVLASSFHVSTYFDRIQE+LF+ Y+IETLSGPP +EL + EEE+ +  DE+
Sbjct: 365 LWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEI 424

Query: 534 QRLQSAGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDPKLSRVSTKNGSE-GINI 593
            ++Q  G  + P+L +AA    KSG  +             + K S +  K GS+ GI +
Sbjct: 425 YKMQKGGADLSPELCSAAFPQEKSGSTM-------------NMKFSPIIPKTGSDNGITM 484

Query: 594 DHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAK 653
           D LHK+  KNVSAWNMKRL+KIVR  S++TLDEQ    + +DES T +I+SE+EAKAAA+
Sbjct: 485 DDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCEDES-TRQIRSEKEAKAAAR 544

Query: 654 KIFQNVARPGFNR---------------------FEGATETRRISKSALKNWVVNAFRER 713
           KIF+NVA+PG                        FEGA  T++I+KSALKNW+VNAFRER
Sbjct: 545 KIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRER 604

Query: 714 RALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASRKVILYISSQIVLVAFIFG 773
           RAL+LTLNDTKTAV +L  +++ + +I IIV+W+ILL IA+ K +L+++SQ+VL+AF+FG
Sbjct: 605 RALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFG 664

Query: 774 NTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLA 833
           N+LK +FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTTVFLR DNLKI+ PN +L 
Sbjct: 665 NSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLW 724

Query: 834 TKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIESDKEHWCPSPIILMKDID 893
            K IHN+ RSPDMG+ +  CVHI TP EKI A+KQRI SYI+S  E+W P   +++KD++
Sbjct: 725 QKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVE 784

Query: 894 SADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFDI 906
             + + ++IWL H +NHQ++ ER+ RR++++EEV+KI  E DI
Sbjct: 785 DLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELDI 809

BLAST of Cp4.1LG04g04760 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 680.6 bits (1755), Expect = 1.7e-195
Identity = 407/907 (44.87%), Postives = 555/907 (61.19%), Query Frame = 0

Query: 59  EPSGHNEVILKVDDGGSSAVSRSQESA-GYGGKVWRESRYDFWSNNDGIGTGGSASWASG 118
           + +   + I+ ++   S AV  +  S+   GG +W+ES YDFW    G          + 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEKG---------KND 64

Query: 119 ARASDSGDRNEGFEFVRRGN------GTDDPPTKLIGDFLHKQKVRG-ETTLDLDLEMEE 178
            +  D  +    F F +RG          DPP+KLIG FLHKQ+  G E +LD++L M E
Sbjct: 65  KKGDDEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAE 124

Query: 179 LRLDRTKTPMMDSPLSRASKDLKVSFQHDSTEISSN---ESVRRRYKEFREMQEESKRGQ 238
           L   ++ TP   +  S   +    +    S+ + +    ++VRRR               
Sbjct: 125 L---QSNTPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRR--------------- 184

Query: 239 PSWHQSHHEHGGSSISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPP 298
               Q+    GGSS    +N   AE ++  S           +K  L + K KSRL DPP
Sbjct: 185 ----QNRTSLGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPP 244

Query: 299 A-------EPDRLSGLVNKSGQLRSGFLGRT------------DDEEDDPFLEEDFPDDF 358
                   + +  SG   +SG  +SGFLG++            ++EE+DPFL+ED P++F
Sbjct: 245 TPTHPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEF 304

Query: 359 RRGKLDALTVLQWISLFLITAALVCTLSIRHLRENSLWEFSIWKWEVMVLVLICGRLVSG 418
           +R KL     L+WISL LI  +LVC+L+I +L+  + W+  +WKWEV VLVLICGRLVS 
Sbjct: 305 KRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSS 364

Query: 419 WGIGIGVFFIERNFLLRKKVLYFVYGIKRPVQNCLWLGLVLIAWHLLFDKRVKNQTNSDI 478
           W + I VF +E+NF  RK+VLYFVYG+++ VQNCLWLGLVL+AWH LFDK+V+ +T S  
Sbjct: 365 WIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTA 424

Query: 479 LDYVRKSLVCLLLSTLIWLAKTLMLKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPRI 538
           L                                  TYFDRIQESLF QYVIETLSGPP +
Sbjct: 425 L---------------------------------RTYFDRIQESLFTQYVIETLSGPPLM 484

Query: 539 ELQKSEEEQKKIADEVQRLQS-AGVPVPPDLRAAALSSIKSGERVRSNVASQRSLRGKDP 598
           E+Q+ EEE++++A++V+ L+  AG  +PP L+A   S +K                GK P
Sbjct: 485 EIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKV---------------GKSP 544

Query: 599 KLSRVSTKNG--SEGINIDHLHKLTSKNVSAWNMKRLLKIVRYGSITTLDEQIQGPSLDD 658
            L+R+ +K G   EGI ID L ++ +KNVSAWNMKRL+ I+  G+I+TLD+ +Q  + +D
Sbjct: 545 GLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQED 604

Query: 659 ESTTTEIKSEREAKAAAKKIFQNVARPG--------FNR-------------FEGATETR 718
           E   T I+SE EAK AA+KIF NV  PG        F R             FEGA+E+ 
Sbjct: 605 ED-ATHIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESD 664

Query: 719 RISKSALKNWVVNAFRERRALSLTLNDTKTAVERLGLVVNVIFSIFIIVLWIILLGIASR 778
           +ISKS LKNWV  AFRERRAL+LTLNDTKTAV+RL  ++NV+  I II++W+++LGIA+ 
Sbjct: 665 KISKSCLKNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATT 724

Query: 779 KVILYISSQIVLVAFIFGNTLKNIFEAIIFLFVMHPFDVGDRCEIDDIQMVVEEMNILTT 838
           + +L +SSQ++LVAF+FGN+ K IFEAIIFLFVMHPFDVGDRCEID +Q+VVEEMNILTT
Sbjct: 725 RFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTT 784

Query: 839 VFLRHDNLKIIVPNSVLATKVIHNHYRSPDMGEFIEICVHIMTPLEKITAMKQRIISYIE 898
           VFLR+DN KII PNSVL TK I N+YRSPDMG+ +E CVHI TP EKITA+KQRI+SY++
Sbjct: 785 VFLRYDNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVD 816

Query: 899 SDKEHWCPSPIILMKDIDSADKLAMSIWLSHTMNHQDIRERWARRSVVVEEVLKICQEFD 912
           + K++W P+P+I+   +D  + + +++WL+H MNHQD+ ER+ RR +++EEV K C+E D
Sbjct: 845 NKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELD 816