Cp4.1LG04g04580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g04580 vs. ExPASy Swiss-Prot
Match: Q9HGL2 (Uncharacterized calcium-binding protein C800.10c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC800.10c PE=4 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.5e-15
Identity = 118/463 (25.49%), Postives = 182/463 (39.31%), Query Frame = 0

Query: 7   SAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRA 66
           SA     FD  F+ AD    G I+G EAV F + SGL  QVL QIW ++D    GFL  +
Sbjct: 6   SAEEQTAFDQLFKIADKQDIGVITGEEAVPFLEKSGLAPQVLGQIWQIADAENRGFLTFS 65

Query: 67  EFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIP-APQINFNTPPASQFNSTPALPTP 126
            F  A+RLV +AQ K     +          + KIP    I+ +   +S+F         
Sbjct: 66  GFVIAMRLVALAQEKLPFDYK---------KSGKIPYFADIHISGVDSSKF--------- 125

Query: 127 QTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGFSGVGP 186
              +    P+ VS  +                  + S  PP+++     +  Q F+ V P
Sbjct: 126 ---VQLNRPNNVSSGDG----------------SDGSFLPPISSDEMTRYQ-QMFTTVCP 185

Query: 187 LSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSLPPRPQ 246
             G              DRA  + G          +P  T+                   
Sbjct: 186 TDGLMD----------GDRASSIFGR---------APLSTE------------------- 245

Query: 247 TAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTGNISST 306
               I +   + VD++ +G     +    +  G H              + L  G++ S 
Sbjct: 246 ----ILARVWNLVDTHKRG----ALDIREFNTGMHII------------NLLLNGSLKSP 305

Query: 307 SVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASSGLSTG 366
               PV  +   I  A S  S+      P L+  PP   P         + P S      
Sbjct: 306 ----PVSISPSFIASAASTSSVSAPSQYPGLSRSPPVQAP---------NIPVSD----- 342

Query: 367 SQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPREVLKQ 426
                      PW   +Q D+  + ++F  VDK   G ++G EA + FL+ +LP +VL Q
Sbjct: 366 -----------PWAIPSQ-DLTSFCQLFSNVDKAHKGYVSGSEAYSFFLASKLPEDVLAQ 342

Query: 427 VWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVV 469
           +WDLSD +++  L++ EFCI+LYL++    G  LP +LPS+++
Sbjct: 426 IWDLSDTNSNGKLNIGEFCISLYLIKLKLSGKELPKVLPSSML 342

BLAST of Cp4.1LG04g04580 vs. ExPASy Swiss-Prot
Match: A5DP36 (Actin cytoskeleton-regulatory complex protein PAN1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=PAN1 PE=3 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 1.4e-10
Identity = 135/502 (26.89%), Postives = 189/502 (37.65%), Query Frame = 0

Query: 7   SAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRA 66
           +A +   F+  FR A    +  ISG  A      SGLP   LA+IW+LSD  + G L   
Sbjct: 114 TASDQSKFEHLFRTAVRKGETAISGDAARDILLRSGLPPVTLAEIWSLSDTNKSGSLLFP 173

Query: 67  EFNNALRLVTVAQSKREL---TPE--------IVKAALFS----PAASKIPAPQINFNTP 126
           EF  +L L  +A     L    PE         V A  FS    P+      P  +  T 
Sbjct: 174 EFALSLHLCNLALKSEPLPSVLPEKWSNEVQSFVDAISFSVPEDPSKILANTPFASLGTE 233

Query: 127 PASQFNSTPALPT----PQ-TGIGTP-TPSQVSGLE---------SQVARSVTTTASPVP 186
             + +   PA P+    PQ TG   P  P+Q +G +         S +      TA  VP
Sbjct: 234 EKTSWMDAPAAPSTSFQPQLTGFQPPQMPAQRTGGQITAQRTGGGSLIPLQPQQTAGLVP 293

Query: 187 S---RENQSVRP--PLATSNSAFHPVQGFSGVGPLSGPPPTNSSISNDWVSDRAGGVQGT 246
           +   R+N   +P  PL + N+ F    G    G     PP                 QGT
Sbjct: 294 AGLQRQNTGYQPPQPLQSQNTGFQSSVGRQNTG---YQPPLQQ--------------QGT 353

Query: 247 PSQP---PNRGFSPAGTQVGFGQSSAGLITSLPPRPQTAPGIKSATPSPVDSNVQGVSGN 306
             QP    N G+ P   Q G G          PP  Q   G +     P+    QG    
Sbjct: 354 GYQPLKQQNTGYQPPLQQQGTGYQ--------PPLQQQGTGYQ----PPLQQ--QGTGYQ 413

Query: 307 GIASGSYFGGGHFAASSVPSKQDVPSDKLSTGNISSTSVTVPVPSATQPIVRAGSLDSLQ 366
            + S     G  F +      Q       STG         P   ++QP  + G     Q
Sbjct: 414 PLQS----QGTGFQSQGTIQPQGTGFQPQSTG-----FQPQPTGLSSQPTGKPG-----Q 473

Query: 367 NSFMKPPLANQPPRNQPLGKPNQQSISHPASSGLSTGSQNSVSG--QSQRPWPRMTQTDV 426
             F+  P    P      G    +    P +   S   QN++ G  ++   W  +T+ + 
Sbjct: 474 WGFVSTPTGGIP------GLSAMEQHFLPNTQLSSNNLQNAMGGSLKTNVTW-AITKQEK 533

Query: 427 QKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIA 469
             Y  +F   DK R G I GE A  +F    L R  L+ +W+L+D DN   L+  EF +A
Sbjct: 534 SIYDGIFQAWDKSRQGFIDGETAIGIFGKSGLARPDLETIWNLADGDNKGKLNKDEFSVA 563

BLAST of Cp4.1LG04g04580 vs. ExPASy Swiss-Prot
Match: Q6C908 (Actin cytoskeleton-regulatory complex protein PAN1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=PAN1 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.9e-10
Identity = 124/505 (24.55%), Postives = 184/505 (36.44%), Query Frame = 0

Query: 14  FDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNALR 73
           F+  FR+A    +  +SG +A      SGLP   L+ IW L+D  + G L   EF  A+ 
Sbjct: 237 FENLFRQALPKGEQALSGDKARDILFRSGLPPITLSAIWNLADTTRSGALLFPEFAVAMY 296

Query: 74  LVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTPALPTPQTGIGTPT 133
           L   A  K +  P  +   + +  +S +    I+FN P A    S+     PQ+      
Sbjct: 297 LCGQA-VKGQTVPNNLSENIKNEVSSMVDI--ISFNIPDAGSRPSSSGQSVPQS-----Q 356

Query: 134 PSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGFSGVGPLSGPPPTN 193
           P Q     S    +      P   ++ Q+         S  +P+Q       ++G  P  
Sbjct: 357 PQQQQQQSSASMLAGLNLGQPTGYQQQQATGYQPMQQQSTGYPMQAMQ--PQITGGMPLQ 416

Query: 194 SSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSLPPRPQTAPGIKSA 253
                     R G +Q  P Q  + G++P  +Q+  G      +T   P      G    
Sbjct: 417 --------QQRTGPMQ--PLQQQSTGYAPLQSQLTGGAPLQSQLTGGAPLQSQLTG---- 476

Query: 254 TPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTGNISSTSVTVPVPS 313
             +P+ S + G +     S  Y      A     S    P  + STG +     T   P 
Sbjct: 477 -GAPLQSQLTGGAPLQQQSTGY------APLQQQSTGYAPLQQQSTGYMPQQQ-TGMQPQ 536

Query: 314 ATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASSGLSTGSQNSVSGQ 373
           +T      GS+              QP    P GKP Q    +  S GL       +   
Sbjct: 537 ST----GYGSM--------------QPLTAMPTGKPGQWGFINTPSQGLP-----GIETM 596

Query: 374 SQRPWPRMTQTDVQK-----------------------YTKVFVVVDKDRDGKITGEEAR 433
            QR  P+ T   VQ+                       Y  +F+  DK R G I G+ A 
Sbjct: 597 QQRLMPQATGAPVQQLPPMQLQQSATVNWAIAKEEKQIYDGIFMAWDKKRAGAIDGDTAI 656

Query: 434 NLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFD 493
            +F    L R  L+ +W LSD  N   L   EF +A++L+ RH  G+ +P+ LP  +V  
Sbjct: 657 KIFTQSGLNRADLEAIWTLSDPSNKGRLDRDEFAVAMHLIYRHLNGYPIPSRLPPELVPP 686

Query: 494 FSSNGHPVTPAASNYSNAAWRPTAG 496
            S N         +Y  A    T G
Sbjct: 717 SSKNFSDSVNQVKSYLKAGGGRTGG 686

BLAST of Cp4.1LG04g04580 vs. ExPASy Swiss-Prot
Match: Q6BNL1 (Actin cytoskeleton-regulatory complex protein PAN1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=PAN1 PE=3 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-08
Identity = 126/496 (25.40%), Postives = 186/496 (37.50%), Query Frame = 0

Query: 7   SAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRA 66
           +A +   F+  FR A    +  ISG  A      SGL    LA+IW+L+D  + G L   
Sbjct: 125 TAADQSKFEHLFRTAVPKGEQAISGDSARDILLRSGLQPITLAEIWSLADTNKSGSLLFP 184

Query: 67  EFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTP--PASQFNSTP---- 126
           EF  AL L  ++  K +  P ++     +   S + A  I+F+ P  PA+  ++TP    
Sbjct: 185 EFALALHLCNLS-LKGDPLPTMLPEKWSNEVKSFVDA--ISFSVPENPANILSNTPFASS 244

Query: 127 ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSV------RPPLATSNSAF 186
              +         P       S    S +  A P     +Q +       PPL    + F
Sbjct: 245 GASSNPINNDWMAPQATGFNNSGAVPSTSFQAQPTGFGASQEMMAQRTGNPPLPQQATGF 304

Query: 187 HPVQGFSGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSA 246
               G + V PL              +  R GG    P QP               Q ++
Sbjct: 305 ----GSNNVAPL--------------LPQRTGGGTLIPLQP---------------QQTS 364

Query: 247 GLITSL---PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQD 306
            LI +    P +PQT  G ++  P             G A     G              
Sbjct: 365 NLIPAQKTGPLQPQTT-GFQTQNPHQTGPGALQPQSTGFAQRMNNG-------------- 424

Query: 307 VPSDKLSTGNISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQ 366
            P    +TG    T+   P  +  QP  ++         F   P    P + QP GKP Q
Sbjct: 425 -PLQAQTTGFQQQTTGFQPQSTGFQP--QSTGFQPQSTGFQ--PQQTGPLQAQPTGKPGQ 484

Query: 367 QS-ISHPA---------------SSGLSTGS-QNSVSG--QSQRPWPRMTQTDVQKYTKV 426
              +S P                SS L T + QN++ G  ++   W  +T+ + Q Y  V
Sbjct: 485 WGFVSTPTGGIPGMNAMEQHFLPSSQLPTNNLQNAMGGSLKTNVTW-SITKQEKQIYDGV 544

Query: 427 FVVVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIALYLLER 469
           F   D    G I GE A N+F    L R  L+ +W+L+D +N   L+  EF +A++L+ R
Sbjct: 545 FSAWDSRNKGFIDGEVAINIFGKSGLARPDLESIWNLADTNNRGKLNKDEFAVAMHLVYR 563

BLAST of Cp4.1LG04g04580 vs. ExPASy Swiss-Prot
Match: Q9Z0R4 (Intersectin-1 OS=Mus musculus OX=10090 GN=Itsn1 PE=1 SV=2)

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-07
Identity = 93/385 (24.16%), Postives = 166/385 (43.12%), Query Frame = 0

Query: 306 SVTVPVPSATQPIV-RAGSLDSLQNSFMKPPLAN-QPPRNQPLGKPNQQSISHPASSGLS 365
           +   PVP  + P+V  +  L S       PPLAN  PP  QPL      + + P SS  S
Sbjct: 127 TAVAPVPMGSIPVVGMSPPLVSSVPPAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFS 186

Query: 366 ---TGSQNSVSGQSQRPWP----------RMTQTDVQKYTKVFVVVDKDRDGKITGEEAR 425
               GSQ +   Q  + +            + Q+   KY ++F   DK   G +TG +AR
Sbjct: 187 RSGPGSQLNTKLQKAQSFDVASAPPAAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQAR 246

Query: 426 NLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVV-- 485
            + +   LP+  L  +W+LSD D D  L+  EF +A++L++    G  LP +LP   +  
Sbjct: 247 TILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPP 306

Query: 486 -FDFSSNGHPVTPAASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPV 545
            F    +G  ++  +S+  +         Q+    P S +        E+ P        
Sbjct: 307 SFRRVRSGSGMSVISSSSVD---------QRLPEEPSSED--------EQQPEKKLPVTF 366

Query: 546 EDEPQTNKPKSKVEELEKEILESRQKIEYYRTKMQELVLYKSRCDNRLNEISERVSSEKR 605
           ED+ + N  +  V ELEK     RQ +   + K QE +    R +    E   +    KR
Sbjct: 367 EDKKRENFERGSV-ELEKR----RQALLEQQRKEQERLAQLERAEQERKERERQEQERKR 426

Query: 606 EVE---SFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMELYHAIVKME----QDVSE 665
           ++E      K+ E + ++  +    +   EA  R+++ ++   +    + E    ++  +
Sbjct: 427 QLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQ 486

HSP 2 Score: 48.5 bits (114), Expect = 5.8e-04
Identity = 46/154 (29.87%), Postives = 69/154 (44.81%), Query Frame = 0

Query: 27  GRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNALRLVTVAQSKRELTP 86
           G I+G +A  FF  SGLP+ VLAQIWAL+D    G + + EF+ A++L+ +     +L  
Sbjct: 37  GFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPS 96

Query: 87  EIVKAALFSPAA-SKIPAPQIN--FNTPPASQFNSTPALPTPQTGIGTPTPSQV-SGLES 146
            +       P A S  PA  I    + PP +     P    P  G+  P  S V      
Sbjct: 97  TLPPVMKQQPVAISSAPAFGIGGIASMPPLTAVAPVPMGSIPVVGMSPPLVSSVPPAAVP 156

Query: 147 QVARSVTTTASPVPSRENQSVRPPLATSNSAFHP 177
            +A        P+P+  + +   P ++S S   P
Sbjct: 157 PLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGP 190

BLAST of Cp4.1LG04g04580 vs. NCBI nr
Match: XP_023529584.1 (actin cytoskeleton-regulatory complex protein PAN1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529585.1 actin cytoskeleton-regulatory complex protein PAN1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529586.1 actin cytoskeleton-regulatory complex protein PAN1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2225 bits (5766), Expect = 0.0
Identity = 1157/1190 (97.23%), Postives = 1157/1190 (97.23%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEILESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVGQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG
Sbjct: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS
Sbjct: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
            PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS
Sbjct: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1190

BLAST of Cp4.1LG04g04580 vs. NCBI nr
Match: XP_023529588.1 (actin cytoskeleton-regulatory complex protein PAN1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2169 bits (5619), Expect = 0.0
Identity = 1135/1190 (95.38%), Postives = 1135/1190 (95.38%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEILESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVGQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                      FDASPRDKDTQYRDSNLGKAAG
Sbjct: 841  ESPKEHRDSNFGKAAG----------------------FDASPRDKDTQYRDSNLGKAAG 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS
Sbjct: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
            PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS
Sbjct: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1168

BLAST of Cp4.1LG04g04580 vs. NCBI nr
Match: KAG7022768.1 (hypothetical protein SDJN02_16504 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2074 bits (5374), Expect = 0.0
Identity = 1091/1190 (91.68%), Postives = 1099/1190 (92.35%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFD YFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALPTPQTGIGTPTPSQ SGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPTPQTGIGTPTPSQGSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGP+SGPPPTNSSISNDWVSD AGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPVSGPPPTNSSISNDWVSDHAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQTAPGIKSATPSPVDSNVQG SGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQTAPGIKSATPSPVDSNVQGFSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTS TVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSATVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVPGSGN+H APVVG +PPTPAAASPVEDEPQ+NKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNVHVAPVVGVQPPTPAAASPVEDEPQSNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEI ESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVSQLSTEEQNSLNSKFQEAADAEKKVEELEKEISESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKMEQDVSEDGIIQVRADRIQADIE+LVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDG STPDADSK EKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                                            
Sbjct: 841  ESPKEHRDSNFGKAAG-------------------------------------------- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFD SPRDKDALSDHGG GSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDASPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DD+DSVWGFNAGGSTKTD DVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDVDSVWGFNAGGSTKTDTDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPNNYHEGSEPSFE+FSRFDSSSVHEGGFPPQDTFARFDSMRSS+DFDQGPGFS
Sbjct: 1021 LFNSGNSPNNYHEGSEPSFESFSRFDSSSVHEGGFPPQDTFARFDSMRSSKDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
            PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS
Sbjct: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1146

BLAST of Cp4.1LG04g04580 vs. NCBI nr
Match: XP_022928490.1 (epidermal growth factor receptor substrate 15-like [Cucurbita moschata] >XP_022928491.1 epidermal growth factor receptor substrate 15-like [Cucurbita moschata] >XP_022928492.1 epidermal growth factor receptor substrate 15-like [Cucurbita moschata])

HSP 1 Score: 2067 bits (5356), Expect = 0.0
Identity = 1086/1190 (91.26%), Postives = 1097/1190 (92.18%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFD YFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFN TP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNPTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALP PQTGIGTPTPSQ SGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPKPQTGIGTPTPSQGSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGP+SGPPPTNSSISNDWVSD AGGVQGTPSQPPN GFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPVSGPPPTNSSISNDWVSDHAGGVQGTPSQPPNIGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQTAPGIKSATPSPVDSNVQG SGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQTAPGIKSATPSPVDSNVQGFSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTS TVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSATVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHP TP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPATP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVPGSGN+HGAPVVG +PPTPAAASPVEDEPQ+NKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNVHGAPVVGVQPPTPAAASPVEDEPQSNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEI ESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVSQLSTEEQNSLNSKFQEAADAEKKVEELEKEISESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKME+DVSEDGIIQVRADRIQADIE+LVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEEDVSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDG STPDADSK EKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                                            
Sbjct: 841  ESPKEHRDSNFGKAAG-------------------------------------------- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGG GSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DD+DSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDVDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPNNYHEGSEPSFE+FSRFDSSSVHEGGFPP+DTFARFDSMRSS+DFDQGPGFS
Sbjct: 1021 LFNSGNSPNNYHEGSEPSFESFSRFDSSSVHEGGFPPRDTFARFDSMRSSKDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
            PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFP+LS
Sbjct: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPTLS 1140

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSM SSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMHSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1146

BLAST of Cp4.1LG04g04580 vs. NCBI nr
Match: XP_022989290.1 (actin cytoskeleton-regulatory complex protein PAN1-like [Cucurbita maxima] >XP_022989291.1 actin cytoskeleton-regulatory complex protein PAN1-like [Cucurbita maxima] >XP_022989293.1 actin cytoskeleton-regulatory complex protein PAN1-like [Cucurbita maxima])

HSP 1 Score: 1989 bits (5154), Expect = 0.0
Identity = 1056/1190 (88.74%), Postives = 1068/1190 (89.75%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFD YFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKR+LTP+IVKAALFSPAASKIPAPQINFNTPPASQFNSTP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRDLTPDIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALPTPQTGIGTPTP Q S LESQVARSVTT  SPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPTPQTGIGTPTPPQSSRLESQVARSVTTNVSPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGP+SG PPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPVSGSPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQ APGIKSATPSPVDSNVQG S N IASGSY GGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQAAPGIKSATPSPVDSNVQGFSENRIASGSYIGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTSVTVPVPSATQPIVRAGSLDS QNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSVTVPVPSATQPIVRAGSLDSSQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVF+FSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFNFSSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVP SGNLHGAP+VG RPPTPAAAS VEDEPQTNKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPESGNLHGAPIVGVRPPTPAAASLVEDEPQTNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEILESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVSQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKMEQD SEDGIIQVRADRIQADIE+LVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEQDGSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKS+S QKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSDSAQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDG STPDADSK EKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                                            
Sbjct: 841  ESPKEHRDSNFGKAAG-------------------------------------------- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFD SPRDKDALSDHGG GSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDASPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DDIDSVWGFNAGGSTKTDNDV +DNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDIDSVWGFNAGGSTKTDNDVIQDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPN+YHEGSEPSFE+FSRFDSSSVHEGGFPP+DTFARFDSM SSRDFDQGPGFS
Sbjct: 1021 LFNSGNSPNSYHEGSEPSFESFSRFDSSSVHEGGFPPRDTFARFDSMHSSRDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
             FGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQ                   EFPSLS
Sbjct: 1081 SFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQ-------------------EFPSLS 1127

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSMRSSKDFDQGHGFPSFDDADFGSTGPF+ASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFQASLDNQTPKKGSDNWSAF 1127

BLAST of Cp4.1LG04g04580 vs. ExPASy TrEMBL
Match: A0A6J1EK34 (epidermal growth factor receptor substrate 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435284 PE=4 SV=1)

HSP 1 Score: 2067 bits (5356), Expect = 0.0
Identity = 1086/1190 (91.26%), Postives = 1097/1190 (92.18%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFD YFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFN TP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNPTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALP PQTGIGTPTPSQ SGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPKPQTGIGTPTPSQGSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGP+SGPPPTNSSISNDWVSD AGGVQGTPSQPPN GFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPVSGPPPTNSSISNDWVSDHAGGVQGTPSQPPNIGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQTAPGIKSATPSPVDSNVQG SGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQTAPGIKSATPSPVDSNVQGFSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTS TVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSATVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHP TP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPATP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVPGSGN+HGAPVVG +PPTPAAASPVEDEPQ+NKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNVHGAPVVGVQPPTPAAASPVEDEPQSNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEI ESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVSQLSTEEQNSLNSKFQEAADAEKKVEELEKEISESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKME+DVSEDGIIQVRADRIQADIE+LVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEEDVSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDG STPDADSK EKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                                            
Sbjct: 841  ESPKEHRDSNFGKAAG-------------------------------------------- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGG GSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DD+DSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDVDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPNNYHEGSEPSFE+FSRFDSSSVHEGGFPP+DTFARFDSMRSS+DFDQGPGFS
Sbjct: 1021 LFNSGNSPNNYHEGSEPSFESFSRFDSSSVHEGGFPPRDTFARFDSMRSSKDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
            PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFP+LS
Sbjct: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPTLS 1140

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSM SSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMHSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1146

BLAST of Cp4.1LG04g04580 vs. ExPASy TrEMBL
Match: A0A6J1JPV3 (actin cytoskeleton-regulatory complex protein PAN1-like OS=Cucurbita maxima OX=3661 GN=LOC111486407 PE=4 SV=1)

HSP 1 Score: 1989 bits (5154), Expect = 0.0
Identity = 1056/1190 (88.74%), Postives = 1068/1190 (89.75%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQNSAPNVDLFD YFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI
Sbjct: 1    MASAQNSAPNVDLFDAYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEFNNALRLVTVAQSKR+LTP+IVKAALFSPAASKIPAPQINFNTPPASQFNSTP
Sbjct: 61   GFLGRAEFNNALRLVTVAQSKRDLTPDIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            ALPTPQTGIGTPTP Q S LESQVARSVTT  SPVPSRENQSVRPPLATSNSAFHPVQGF
Sbjct: 121  ALPTPQTGIGTPTPPQSSRLESQVARSVTTNVSPVPSRENQSVRPPLATSNSAFHPVQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVGP+SG PPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL
Sbjct: 181  SGVGPVSGSPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQ APGIKSATPSPVDSNVQG S N IASGSY GGGHFAASSVPSKQDVPSDKLSTG
Sbjct: 241  PPRPQAAPGIKSATPSPVDSNVQGFSENRIASGSYIGGGHFAASSVPSKQDVPSDKLSTG 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            NISSTSVTVPVPSATQPIVRAGSLDS QNSFMKPPLANQPPRNQPLGKPNQQSISHPASS
Sbjct: 301  NISSTSVTVPVPSATQPIVRAGSLDSSQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR
Sbjct: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVF+FSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFNFSSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASNYSNAAWRPTAGYQQHQGVP SGNLHGAP+VG RPPTPAAAS VEDEPQTNKPKSKV
Sbjct: 481  AASNYSNAAWRPTAGYQQHQGVPESGNLHGAPIVGVRPPTPAAASLVEDEPQTNKPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEILESRQKIEYYRTKMQELVLY
Sbjct: 541  PVLEKNLVSQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVLY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YHAIVKMEQD SEDGIIQVRADRIQADIE+LVKSLNERCKSYGLRAKPITLSELPFGWQP
Sbjct: 661  YHAIVKMEQDGSEDGIIQVRADRIQADIEELVKSLNERCKSYGLRAKPITLSELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKS+S QKEQGSAIDSQKV
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSDSAQKEQGSAIDSQKV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPAPDVDTKDG STPDADSK EKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI
Sbjct: 781  TPAPDVDTKDGGSTPDADSKGEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
            ESPKEHRDSNFGKAAG                                            
Sbjct: 841  ESPKEHRDSNFGKAAG-------------------------------------------- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
            FDASPRDKDTQYRDSNLGKAAGFD SPRDKDALSDHGG GSVFSGDKSYDEPAWGTFDMN
Sbjct: 901  FDASPRDKDTQYRDSNLGKAAGFDASPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFDMN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DDIDSVWGFNAGGSTKTDNDV +DNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP
Sbjct: 961  DDIDSVWGFNAGGSTKTDNDVIQDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFS 1080
            LFNSGNSPN+YHEGSEPSFE+FSRFDSSSVHEGGFPP+DTFARFDSM SSRDFDQGPGFS
Sbjct: 1021 LFNSGNSPNSYHEGSEPSFESFSRFDSSSVHEGGFPPRDTFARFDSMHSSRDFDQGPGFS 1080

Query: 1081 PFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPSLS 1140
             FGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQ                   EFPSLS
Sbjct: 1081 SFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQ-------------------EFPSLS 1127

Query: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFRASLDNQTPKKGSDNWSAF 1157
            RFDSMRSSKDFDQGHGFPSFDDADFGSTGPF+ASLDNQTPKKGSDNWSAF
Sbjct: 1141 RFDSMRSSKDFDQGHGFPSFDDADFGSTGPFQASLDNQTPKKGSDNWSAF 1127

BLAST of Cp4.1LG04g04580 vs. ExPASy TrEMBL
Match: A0A6J1GLI1 (epidermal growth factor receptor substrate 15-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111455055 PE=4 SV=1)

HSP 1 Score: 1727 bits (4473), Expect = 0.0
Identity = 936/1193 (78.46%), Postives = 996/1193 (83.49%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQN APNVDLFD YFRRADLDRDGRISGAEAV+FFQGSGLPK VL QIWALSDPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKPVLFQIWALSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEF NALRLVTVAQSKRELTP+IVKAALFSPAASKIPAPQINFNT PA QFNST 
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAASKIPAPQINFNTQPAPQFNSTA 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
             + TP +GIGTPTPS+ SGLE QV R+VTT    V SRE+Q VRPPLATSNSAF P QGF
Sbjct: 121  PVSTPLSGIGTPTPSRSSGLELQVPRNVTTNVPLVSSRESQLVRPPLATSNSAFRPAQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
            SGVG +SGPPPTNS ISNDWVS+RA G+QGTPSQPPNRG SPAG QVGFGQSSAGL TS 
Sbjct: 181  SGVGTVSGPPPTNSIISNDWVSERASGIQGTPSQPPNRGVSPAGMQVGFGQSSAGLTTSQ 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            P RPQ+APG+  A  SPV+S VQG+SGNG ASGSYFG   F A+ V SKQDVP+     G
Sbjct: 241  PQRPQSAPGVMPAASSPVESKVQGISGNGTASGSYFGRDAFPATPVSSKQDVPA-----G 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            N +STSVTVPV S TQPIVRA SLDSLQNSFMKPPLANQ  RNQP  KPNQQSI  PASS
Sbjct: 301  NKTSTSVTVPVSSVTQPIVRATSLDSLQNSFMKPPLANQALRNQPHVKPNQQSILQPASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
            GLSTG QNSV GQSQRPWPRMTQ DVQKYTKVFV VDKDRDGKIT +EARNLFLSWRLPR
Sbjct: 361  GLSTGLQNSVYGQSQRPWPRMTQNDVQKYTKVFVEVDKDRDGKITAQEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVL QVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSN++FDF SNGHPVTP
Sbjct: 421  EVLMQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFPSNGHPVTP 480

Query: 481  AASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSKV 540
            AASN+SNAAWR TAG+QQHQGVPGSGNLHGAP +G RPP PAAA PVEDEPQTN+PKSKV
Sbjct: 481  AASNHSNAAWRTTAGHQQHQGVPGSGNLHGAPTIGVRPPIPAAALPVEDEPQTNQPKSKV 540

Query: 541  ---------------------------------EELEKEILESRQKIEYYRTKMQELVLY 600
                                             EELEKEIL+SRQKIEYYRTKMQEL+LY
Sbjct: 541  PLLDKNLVSQLSTEEQNSLNSKFQEAEDAEKKVEELEKEILDSRQKIEYYRTKMQELILY 600

Query: 601  KSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660
            KSRCDNRLNEISERVSSEKREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL
Sbjct: 601  KSRCDNRLNEISERVSSEKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMEL 660

Query: 661  YHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQP 720
            YH IVKMEQD+SEDG++Q RADRIQ+DIE+LVK LNERCKSYGLRAKPITL+ELPFGWQP
Sbjct: 661  YHTIVKMEQDISEDGVLQARADRIQSDIEELVKVLNERCKSYGLRAKPITLTELPFGWQP 720

Query: 721  GIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKV 780
            GIQVGAADWDEDWDKFEEEGF+VVKELTLDVQNVIAPPKQKSKS  VQKE+GS +DSQ V
Sbjct: 721  GIQVGAADWDEDWDKFEEEGFSVVKELTLDVQNVIAPPKQKSKS--VQKEKGSTVDSQNV 780

Query: 781  TPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVI 840
            TPA D D K+G S PDAD+K EKPPS+DE AVENGS HDNKSE+GSA+SAPNSPFA    
Sbjct: 781  TPAADADAKEGGSAPDADTKGEKPPSIDETAVENGSAHDNKSENGSAKSAPNSPFA---- 840

Query: 841  ESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAG 900
              PK    S F              T+   S+        +SP      +  S +G    
Sbjct: 841  --PKSAPSSPFA-------------TKSAPSSPFAPKSAPSSP------FASSVIG---- 900

Query: 901  FDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFDMN 960
               SP+    +Y DS+ GKAAGFD SPR KDALSDHGG GSVFSGDKSYDEPAWG FD N
Sbjct: 901  ---SPK----EYMDSSFGKAAGFDASPRAKDALSDHGGAGSVFSGDKSYDEPAWGAFDAN 960

Query: 961  DDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTP 1020
            DDIDSVWGFNAGGSTK DNDV RDNY+FDSGD GLNPIRT+PFQAKRSTFAFDESVPSTP
Sbjct: 961  DDIDSVWGFNAGGSTKADNDVTRDNYYFDSGDFGLNPIRTDPFQAKRSTFAFDESVPSTP 1020

Query: 1021 LFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGF-PPQDTFARFDSMRSSRDFDQGPGF 1080
            L NSGNSPNNYH+GSEPSF++FSRFDSSSVH+ GF PP+DTF RFDSMRSSRDFDQGPGF
Sbjct: 1021 LVNSGNSPNNYHDGSEPSFDSFSRFDSSSVHDSGFFPPKDTFTRFDSMRSSRDFDQGPGF 1080

Query: 1081 SPFGQFDTSRSSRDFDQGGSS-LTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEFPS 1140
            S F QFDT+ ++RDFDQGGSS LTRFDSMRSSKDFDQG PS SRFDSMRSSKDFDQ FPS
Sbjct: 1081 SSFSQFDTTYNNRDFDQGGSSSLTRFDSMRSSKDFDQGSPSLSRFDSMRSSKDFDQGFPS 1140

Query: 1141 LSRFDSMRSSKDFDQGHGFPSFDDAD-FGSTGPFRASLDNQTPKKGSDNWSAF 1157
            L+RFDSMRSSKDFDQGHGFPSFDD D FGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 LARFDSMRSSKDFDQGHGFPSFDDPDPFGSTGPFRASLDNQTPKKGSDNWSAF 1150

BLAST of Cp4.1LG04g04580 vs. ExPASy TrEMBL
Match: A0A5D3DI91 (Epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G003400 PE=4 SV=1)

HSP 1 Score: 1707 bits (4421), Expect = 0.0
Identity = 933/1195 (78.08%), Postives = 994/1195 (83.18%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQN APNVDLFD YFRRADLDRDGRISGAEAV+FFQGSGLPKQVLAQIW+LSDPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKQVLAQIWSLSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEF NALRLVTVAQSKRELTP+IVKAALFSPAA+KIPAPQINFN  PASQFNST 
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNAQPASQFNSTA 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            A+PTPQ+G+   TPS  SG              PV SRENQSVRPPLA  NSAF P QGF
Sbjct: 121  AVPTPQSGVVAQTPSPSSGAN----------VPPVSSRENQSVRPPLAAPNSAFRPAQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
             GVG +SGPPPTNSSISNDWVS+RA GVQGTPSQPPNRG SPAGTQVGFGQSSAGL  SL
Sbjct: 181  PGVGAVSGPPPTNSSISNDWVSERASGVQGTPSQPPNRGVSPAGTQVGFGQSSAGLTASL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQ+APG+  ATPSP++S VQG++GNG  SGSYFG   F A+ V SKQDVP+     G
Sbjct: 241  PPRPQSAPGVTPATPSPLESKVQGITGNGTVSGSYFGRDAFGATPVSSKQDVPA-----G 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            N +STSV VPV S TQPIVRA SLDSLQ+SFMKPPLANQ  RNQ LGK NQQS+   ASS
Sbjct: 301  NKTSTSVAVPVSSVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKSNQQSVLQSASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
             LS GSQNSVSGQSQRPWPRMTQTDVQKYTKVFV VDKDRDGKITG+EARNLFLSWRLPR
Sbjct: 361  VLSAGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGQEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSN++FDFSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFSSNGHPVTP 480

Query: 481  AASNYSNAAWRP-TAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSK 540
            AASNYSNA WRP TAGYQQHQGVPGSGNL GAP VG RPP PA ASPVE E QT++PKSK
Sbjct: 481  AASNYSNAGWRPPTAGYQQHQGVPGSGNLQGAPTVGGRPPIPATASPVEGEQQTSQPKSK 540

Query: 541  V---------------------------------EELEKEILESRQKIEYYRTKMQELVL 600
            V                                 EELEKEILESRQKIEYYRTKMQELVL
Sbjct: 541  VPVLEKNLISQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVL 600

Query: 601  YKSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME 660
            YKSRCDNRLNEISERVSS+KREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME
Sbjct: 601  YKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME 660

Query: 661  LYHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQ 720
            LY AIVKMEQD S DG++Q RADRIQ+DIE+LVKSLNERCKSYGLRAKPITL+ELPFGWQ
Sbjct: 661  LYQAIVKMEQDGSADGVLQARADRIQSDIEELVKSLNERCKSYGLRAKPITLTELPFGWQ 720

Query: 721  PGIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQK 780
            PGIQVGAADWDEDWDKFE+EGF+VVKELTLDVQNVIAPPKQKSKS  VQK     +DSQ 
Sbjct: 721  PGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKS--VQK---GKVDSQN 780

Query: 781  VTPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPF-ASS 840
            VTPA D DTK+GDS P+AD+K +KPPSMDE AVENGS HDNKSEDGSA+SAPNSPF A S
Sbjct: 781  VTPAADDDTKEGDSAPNADTKRDKPPSMDEAAVENGSAHDNKSEDGSAKSAPNSPFTAKS 840

Query: 841  VIESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKA 900
               SP   +      A G   +P+             A G   SP      +  S +G  
Sbjct: 841  APNSPFALK-----SAPGSPFAPK------------SAPG---SP------FASSIIG-- 900

Query: 901  AGFDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFD 960
                 SP+    +Y DS+ GK AGFD+SPRDKDALSDHGG GSVFSGDKSYDEPAWGTFD
Sbjct: 901  -----SPK----EYMDSHFGKTAGFDSSPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFD 960

Query: 961  MNDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPS 1020
             NDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRT+PFQAKRSTFAFDESVPS
Sbjct: 961  ANDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTDPFQAKRSTFAFDESVPS 1020

Query: 1021 TPLFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGF-PPQDTFARFDSMRSSRDFDQGP 1080
            TPLFNSGNSP+NYHEGSE +F++FSRFD+SSVH+ GF PP++TF+RFDSMRSSRDFDQG 
Sbjct: 1021 TPLFNSGNSPHNYHEGSEANFDSFSRFDTSSVHDSGFFPPRETFSRFDSMRSSRDFDQGS 1080

Query: 1081 GFSPFGQFDTSRSSRDFDQGG-SSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEF 1140
            GFS FGQFDT+ SSRDFDQ G SSLTRFDSMRSSKDFDQGFPS SRFDSM+SSKDFDQ F
Sbjct: 1081 GFSSFGQFDTTHSSRDFDQSGPSSLTRFDSMRSSKDFDQGFPSLSRFDSMQSSKDFDQGF 1138

Query: 1141 PSLSRFDSMRSSKDFDQGHGFPSFDDAD-FGSTGPFRASLDNQTPKKGSDNWSAF 1157
            PS SRFDSMRSSKDFDQGHGFPSFDD D FGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 PSFSRFDSMRSSKDFDQGHGFPSFDDPDPFGSTGPFRASLDNQTPKKGSDNWSAF 1138

BLAST of Cp4.1LG04g04580 vs. ExPASy TrEMBL
Match: A0A1S3BHS4 (epidermal growth factor receptor substrate 15-like 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490209 PE=4 SV=1)

HSP 1 Score: 1707 bits (4421), Expect = 0.0
Identity = 933/1195 (78.08%), Postives = 994/1195 (83.18%), Query Frame = 0

Query: 1    MASAQNSAPNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQI 60
            MASAQN APNVDLFD YFRRADLDRDGRISGAEAV+FFQGSGLPKQVLAQIW+LSDPRQI
Sbjct: 1    MASAQNPAPNVDLFDAYFRRADLDRDGRISGAEAVSFFQGSGLPKQVLAQIWSLSDPRQI 60

Query: 61   GFLGRAEFNNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTP 120
            GFLGRAEF NALRLVTVAQSKRELTP+IVKAALFSPAA+KIPAPQINFN  PASQFNST 
Sbjct: 61   GFLGRAEFYNALRLVTVAQSKRELTPDIVKAALFSPAAAKIPAPQINFNAQPASQFNSTA 120

Query: 121  ALPTPQTGIGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGF 180
            A+PTPQ+G+   TPS  SG              PV SRENQSVRPPLA  NSAF P QGF
Sbjct: 121  AVPTPQSGVVAQTPSPSSGAN----------VPPVSSRENQSVRPPLAAPNSAFRPAQGF 180

Query: 181  SGVGPLSGPPPTNSSISNDWVSDRAGGVQGTPSQPPNRGFSPAGTQVGFGQSSAGLITSL 240
             GVG +SGPPPTNSSISNDWVS+RA GVQGTPSQPPNRG SPAGTQVGFGQSSAGL  SL
Sbjct: 181  PGVGAVSGPPPTNSSISNDWVSERASGVQGTPSQPPNRGVSPAGTQVGFGQSSAGLTASL 240

Query: 241  PPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSDKLSTG 300
            PPRPQ+APG+  ATPSP++S VQG++GNG  SGSYFG   F A+ V SKQDVP+     G
Sbjct: 241  PPRPQSAPGVTPATPSPLESKVQGITGNGTVSGSYFGRDAFGATPVSSKQDVPA-----G 300

Query: 301  NISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSISHPASS 360
            N +STSV VPV S TQPIVRA SLDSLQ+SFMKPPLANQ  RNQ LGK NQQS+   ASS
Sbjct: 301  NKTSTSVAVPVSSVTQPIVRASSLDSLQSSFMKPPLANQAQRNQALGKSNQQSVLQSASS 360

Query: 361  GLSTGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPR 420
             LS GSQNSVSGQSQRPWPRMTQTDVQKYTKVFV VDKDRDGKITG+EARNLFLSWRLPR
Sbjct: 361  VLSAGSQNSVSGQSQRPWPRMTQTDVQKYTKVFVEVDKDRDGKITGQEARNLFLSWRLPR 420

Query: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFDFSSNGHPVTP 480
            EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGH+LPAMLPSN++FDFSSNGHPVTP
Sbjct: 421  EVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHVLPAMLPSNIMFDFSSNGHPVTP 480

Query: 481  AASNYSNAAWRP-TAGYQQHQGVPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSK 540
            AASNYSNA WRP TAGYQQHQGVPGSGNL GAP VG RPP PA ASPVE E QT++PKSK
Sbjct: 481  AASNYSNAGWRPPTAGYQQHQGVPGSGNLQGAPTVGGRPPIPATASPVEGEQQTSQPKSK 540

Query: 541  V---------------------------------EELEKEILESRQKIEYYRTKMQELVL 600
            V                                 EELEKEILESRQKIEYYRTKMQELVL
Sbjct: 541  VPVLEKNLISQLSTEEQNSLNSKFQEAADAEKKVEELEKEILESRQKIEYYRTKMQELVL 600

Query: 601  YKSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME 660
            YKSRCDNRLNEISERVSS+KREVES AKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME
Sbjct: 601  YKSRCDNRLNEISERVSSDKREVESLAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKME 660

Query: 661  LYHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQ 720
            LY AIVKMEQD S DG++Q RADRIQ+DIE+LVKSLNERCKSYGLRAKPITL+ELPFGWQ
Sbjct: 661  LYQAIVKMEQDGSADGVLQARADRIQSDIEELVKSLNERCKSYGLRAKPITLTELPFGWQ 720

Query: 721  PGIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQK 780
            PGIQVGAADWDEDWDKFE+EGF+VVKELTLDVQNVIAPPKQKSKS  VQK     +DSQ 
Sbjct: 721  PGIQVGAADWDEDWDKFEDEGFSVVKELTLDVQNVIAPPKQKSKS--VQK---GKVDSQN 780

Query: 781  VTPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPF-ASS 840
            VTPA D DTK+GDS P+AD+K +KPPSMDE AVENGS HDNKSEDGSA+SAPNSPF A S
Sbjct: 781  VTPAADDDTKEGDSAPNADTKRDKPPSMDEAAVENGSAHDNKSEDGSAKSAPNSPFTAKS 840

Query: 841  VIESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKA 900
               SP   +      A G   +P+             A G   SP      +  S +G  
Sbjct: 841  APNSPFALK-----SAPGSPFAPK------------SAPG---SP------FASSIIG-- 900

Query: 901  AGFDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSYDEPAWGTFD 960
                 SP+    +Y DS+ GK AGFD+SPRDKDALSDHGG GSVFSGDKSYDEPAWGTFD
Sbjct: 901  -----SPK----EYMDSHFGKTAGFDSSPRDKDALSDHGGAGSVFSGDKSYDEPAWGTFD 960

Query: 961  MNDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPS 1020
             NDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRT+PFQAKRSTFAFDESVPS
Sbjct: 961  ANDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTDPFQAKRSTFAFDESVPS 1020

Query: 1021 TPLFNSGNSPNNYHEGSEPSFENFSRFDSSSVHEGGF-PPQDTFARFDSMRSSRDFDQGP 1080
            TPLFNSGNSP+NYHEGSE +F++FSRFD+SSVH+ GF PP++TF+RFDSMRSSRDFDQG 
Sbjct: 1021 TPLFNSGNSPHNYHEGSEANFDSFSRFDTSSVHDSGFFPPRETFSRFDSMRSSRDFDQGS 1080

Query: 1081 GFSPFGQFDTSRSSRDFDQGG-SSLTRFDSMRSSKDFDQGFPSFSRFDSMRSSKDFDQEF 1140
            GFS FGQFDT+ SSRDFDQ G SSLTRFDSMRSSKDFDQGFPS SRFDSM+SSKDFDQ F
Sbjct: 1081 GFSSFGQFDTTHSSRDFDQSGPSSLTRFDSMRSSKDFDQGFPSLSRFDSMQSSKDFDQGF 1138

Query: 1141 PSLSRFDSMRSSKDFDQGHGFPSFDDAD-FGSTGPFRASLDNQTPKKGSDNWSAF 1157
            PS SRFDSMRSSKDFDQGHGFPSFDD D FGSTGPFRASLDNQTPKKGSDNWSAF
Sbjct: 1141 PSFSRFDSMRSSKDFDQGHGFPSFDDPDPFGSTGPFRASLDNQTPKKGSDNWSAF 1138

BLAST of Cp4.1LG04g04580 vs. TAIR 10
Match: AT1G20760.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 694.1 bits (1790), Expect = 1.9e-199
Identity = 505/1214 (41.60%), Postives = 649/1214 (53.46%), Query Frame = 0

Query: 9    PNVDLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEF 68
            PN+D F+ YF+RADLD DGRISGAEAV FFQGSGL KQVLAQIW+LSD    GFL R  F
Sbjct: 6    PNMDQFEAYFKRADLDGDGRISGAEAVGFFQGSGLSKQVLAQIWSLSDRSHSGFLDRQNF 65

Query: 69   NNALRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTPALPTPQTG 128
             N+LRLVTVAQSKR+LTPEIV AAL +PAA+KIP P+IN +           A+P P+  
Sbjct: 66   YNSLRLVTVAQSKRDLTPEIVNAALNTPAAAKIPPPKINLS-----------AIPAPRPN 125

Query: 129  IGTPTPSQVSGLESQVARSVTTTASPVPSRENQSVRPPLATSNSAFHPVQGFSGVGPLSG 188
                T   VSG     A +     +  P ++NQ +RP           + G + + P +G
Sbjct: 126  PAATTVGPVSGFGGPGAPNAIVNQNYFPPQQNQQMRPN--------QGISGLTSLRPAAG 185

Query: 189  PPPTNSSISNDWVSDRAGGVQGTPSQPP-------------NRGFSPAGTQVGFGQSSAG 248
            P    S++S  +     G V   P   P             N  ++ AG   G+     G
Sbjct: 186  PEYRPSALSGQFQPVPVGSVTHPPQPVPTSVSGPGSSTFNLNSLYAGAGNTSGYSSGFGG 245

Query: 249  LITSLPPRPQTAPGIKSATPSPVDSNVQGVSGNGIASGSYFGGGHFAASSVPSKQDVPSD 308
               + P     +PG+K    S +D     VSGNG   G  F       SS   KQ+    
Sbjct: 246  GSLAAP-----SPGLKQ--ESHIDPKALVVSGNG---GDMF-------SSFQQKQEP--- 305

Query: 309  KLSTGNISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRNQPLGKPNQQSIS 368
              +  N S +S  VP  +  QP  +  +LDSLQ++F   P  NQ  + +P         S
Sbjct: 306  --TLSNSSISSAIVPTSAGIQPPTKPNALDSLQSTFSMLPSGNQLQQPRPAASSQPAVSS 365

Query: 369  HPASSGLSTGS-----QNSVSGQSQRPWPRMTQTDVQKYTKVFVVVDKDRDGKITGEEAR 428
               SSGL  GS      ++ +G +Q PWP+M  +DVQKYTKVF+ VD D+DGKITGE+AR
Sbjct: 366  QGPSSGLPPGSAVGSGHSTPAGNNQPPWPKMKPSDVQKYTKVFMEVDSDKDGKITGEQAR 425

Query: 429  NLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFCIALYLLERHREGHILPAMLPSNVVFD 488
            NLFLSWRLPREVLK VW+LSDQDND+MLS+REFCI+LYL+ER+REG  LP  LPS+++FD
Sbjct: 426  NLFLSWRLPREVLKHVWELSDQDNDTMLSLREFCISLYLMERYREGRPLPTALPSSIMFD 485

Query: 489  ---FSSNGHPVTPAASNYSNAAWRPTAGYQQHQGVPGSGNLHGAPVVGERPPTPAAA--- 548
                S +G P    +  Y+NA W    G+ Q    PG G     P  G RPP PA     
Sbjct: 486  ETLLSISGAP----SHGYANAGWGSGQGFVQQ---PGMGARPITPTTGMRPPVPAPGPQP 545

Query: 549  ------------SPVEDEP-----------QTNKPKS-----KVEELEKEILESRQKIEY 608
                        +PV D P            +N P++     KV+E +   ++SR+K++Y
Sbjct: 546  GSGIPPNQPRNQAPVLDAPFANHLGNGYSASSNLPEAAADEEKVDEKQNAYMDSREKLDY 605

Query: 609  YRTKMQELVLYKSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEAT 668
            YRTKMQ++VLYKSRCDNRLNEISER S++KRE E+ AKKYEEKYKQ  ++ S+LT+EEA 
Sbjct: 606  YRTKMQDIVLYKSRCDNRLNEISERASADKREAETLAKKYEEKYKQVAEIGSKLTIEEAR 665

Query: 669  FRDIQEKKMELYHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPI 728
            FR+I+ +KMEL  AIV MEQ  S DG++QVRADRIQ+D+E+L+K+L ERCK +GL     
Sbjct: 666  FREIEGRKMELSQAIVNMEQGGSADGLLQVRADRIQSDLEELMKALTERCKKHGLEVNSK 725

Query: 729  TLSELPFGWQPGIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQK 788
             L +LP GWQPGIQ GAA WDE+WDKFE+EGF    E+T D        K K ++ S +K
Sbjct: 726  ALVDLPAGWQPGIQEGAALWDEEWDKFEDEGFG--NEITFD--------KSKEQNSSGEK 785

Query: 789  EQGSAIDSQKVTPAPDVDTKDGDSTPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARS 848
            E G+                DG   PD+ +                 V DN         
Sbjct: 786  ENGT--------------VDDGSGPPDSPT----------------HVEDN--------- 845

Query: 849  APNSPFASSVIESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQ 908
                PF+    E+   H +S +G     D SP                            
Sbjct: 846  --YGPFS----ETSDRHHESEYGTTHSEDRSP---------------------------- 905

Query: 909  YRDSNLGKAAGFDASPRDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGDKSY 968
             RDS + + A    SP        D + GK + F                         +
Sbjct: 906  -RDSPVSRNATEVPSP--------DYSQGKNSEF-------------------------F 965

Query: 969  DEPAWGT-FDMNDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTE-----PF 1028
            D+  W + FD NDD+DSVWGF+A  S   D       YF    D G N  R +      F
Sbjct: 966  DDSNWASAFDTNDDVDSVWGFDASKSQDGD-------YFGSGDDFGGNTARADSPSSRSF 1019

Query: 1029 QAKR-STFAFDESVPSTPLFNSGNSPNNYHEGS--EPSFENFSRFDSSSVHE--GGFPPQ 1088
             A+R S FAFD+SVPSTPL   GNSP  + + S  + +F++FSRFDS +  E   GF  Q
Sbjct: 1026 GAQRKSQFAFDDSVPSTPLSRFGNSPPRFSDASARDNNFDSFSRFDSFNTSEAGAGFSSQ 1019

Query: 1089 -DTFARFDSMRSSRDFDQGPGFSPFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKDFDQGF 1148
             +  +RFDS+ SS+DF                       GG++ +RFDS+ SS+D   G 
Sbjct: 1086 PERLSRFDSINSSKDF-----------------------GGAAFSRFDSINSSRDV-TGA 1019

Query: 1149 PSFSRFDSMRSSKDFDQEFPSLSRFDSMRSSKDFDQGHGFPSFDDAD-FGSTGPFRASLD 1158
              FSRFDS+ SSKDF    PSLSRFDSM S+KDF   HG+ SFDDAD FGSTGPF+ S D
Sbjct: 1146 EKFSRFDSINSSKDFGG--PSLSRFDSMNSTKDFSGSHGY-SFDDADPFGSTGPFKVSSD 1019

BLAST of Cp4.1LG04g04580 vs. TAIR 10
Match: AT1G21630.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 666.8 bits (1719), Expect = 3.2e-191
Identity = 523/1268 (41.25%), Postives = 667/1268 (52.60%), Query Frame = 0

Query: 12   DLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNA 71
            DLFD YFRRADLD DG ISGAEAVAFFQGS LPK VLAQ+W+ +D ++ G+LGRAEF NA
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYLGRAEFYNA 70

Query: 72   LRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTPALPTPQTGIGT 131
            L+LVTVAQS+RELT EIVKAA++SPA++ IPAP+IN    P+ Q      LP  Q    T
Sbjct: 71   LKLVTVAQSRRELTAEIVKAAIYSPASANIPAPKINLAATPSPQ--PRGVLPATQAQGVT 130

Query: 132  PTPSQVSGLESQVARSVTTTASP--VPSRENQSVR-PPLATSNSAFHPVQGFSGVGPLSG 191
              PS  +G+         +T++   VP ++NQ    PP  T  +   P     G    + 
Sbjct: 131  SMPSVAAGVRGPHMGGTVSTSNQQVVPGQQNQFTGIPPSQTQQNFQSPGMPAGGT---NA 190

Query: 192  PPPTNSSISNDWVSDRAGGVQGT-----PSQPPNRGF---SPAGTQVGFGQSSAGLITSL 251
            P P N  + +DW+S R+ G  G      PS     G    +     +     +  + +S 
Sbjct: 191  PRPANQPMPSDWLSGRSVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSST 250

Query: 252  PPRPQTA-----PGIKSAT--------PS----PVDSNVQGVSGNGIASGSYFGGGHFAA 311
              RPQ +     P   SAT        PS    P D      SGNG  S S F G  F+ 
Sbjct: 251  TTRPQESAPVHNPQESSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLF-GDVFSV 310

Query: 312  SSVPSKQDVPSDKLSTGNISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRN 371
            +S   KQ       +TG  S T+ TV  P  TQ +VR  S+   Q S  +  +  Q    
Sbjct: 311  TSTQPKQHPTGSASTTGISSVTTGTVAGPEITQSVVRQSSIPQ-QGSLSQHAVGVQTQLT 370

Query: 372  QPLGKPNQQS--ISHPASSGLSTG-----------------SQNSVSGQSQRPWPRMTQT 431
               G+P   S   S P  S +  G                  Q    GQSQ PWP+MT  
Sbjct: 371  GNSGQPYTSSGAASGPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPA 430

Query: 432  DVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFC 491
            DVQKYTKVFV VD DRDGKITG +ARNLFLSWRLPR+ LKQVWDLSDQDNDSMLS+REFC
Sbjct: 431  DVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFC 490

Query: 492  IALYLLERHREGHILPAMLPSNVVFD---FSSNGHPVTPAASNYSNAAWRPTAGYQQHQG 551
            IA+YL+ER+REG  LP + PS+++     F+S G  V P    + NA+W    G+QQ   
Sbjct: 491  IAVYLMERYREGRPLPPVFPSSIIHSESMFTSPGQSVAP----HGNASWGHPQGFQQQ-- 550

Query: 552  VPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPK------------------------ 611
             P  G L        +PP P   SP +   Q  +PK                        
Sbjct: 551  -PHPGGLRPPAGPKGKPPRPVPLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNT 610

Query: 612  ---------SKVEELEKEILESRQKIEYYRTKMQELVLYKSRCDNRLNEISERVSSEKRE 671
                      KV+ELEKEI +S+QKI+++R KMQELVLYKSRCDNR NEI+ERV  +KRE
Sbjct: 611  KFEEATAVDKKVDELEKEIADSKQKIDFFRAKMQELVLYKSRCDNRYNEIAERVLGDKRE 670

Query: 672  VESFAKKYEEKYKQSGDVASRLTVEEATFRDIQEKKMELYHAIVKMEQDVSEDGIIQVRA 731
            +ES AKKYEEKYK+SG+V S+LT+EEATFRDIQEKKMELY AIVK E+   +D I++ R 
Sbjct: 671  LESLAKKYEEKYKKSGNVGSKLTIEEATFRDIQEKKMELYQAIVKFEEGKLDDSIVKERT 730

Query: 732  DRIQADIEDLVKSLNERCKSYGLRAKPITLSELPFGWQPGIQVGAADWDEDWDKFEEEGF 791
            + IQ+ +E+L+K+LNERCK YG+R KP +L ELPFGWQPGIQ GAADWDEDWDK E+EGF
Sbjct: 731  EHIQSGLEELIKNLNERCKQYGVRGKPTSLVELPFGWQPGIQEGAADWDEDWDKLEDEGF 790

Query: 792  TVVKELTLDVQNVIAPPKQKSKSESVQKEQGSAIDSQKVTPAPDVDTKDGD--STPDADS 851
            T VKELTLD+QNVIAPPK+  KS + +KE              DV +K+G+  S  DADS
Sbjct: 791  TFVKELTLDIQNVIAPPKE--KSSAWRKE-------------VDVSSKEGEDVSFSDADS 850

Query: 852  KEEKPPSMDEKAVENGSVHDNKSEDGSARSAPNSPFASSVIESPKEHRDSNFGKAAGFDA 911
            K  K  S  E   E+    + K+ D  AR    S              DS   K    D+
Sbjct: 851  KTGKKQSSGE---EDSEQSEGKTSDVDARDKNGS------------LDDSKVRKGIEADS 910

Query: 912  SPRDKDTQYRDSNFGKAAGFDASPRDKDTQYRDSNLGKAAGFDASP---RDKDTQYRDSN 971
            SPR KDT+   S  G   G   +   K   Y DS+    +    +P   +DKD    DS 
Sbjct: 911  SPRTKDTR---SENGHDDGESTASAGKTVNY-DSHDETDSVSSVNPDNGKDKDHGKYDSG 970

Query: 972  LGKAAGFDTSPRDKDALSDHGGVGSVFSGD----------KSYDEPAWGTFDMNDDIDSV 1031
             G   GFD      D        GS  S D           S   P     D++    S+
Sbjct: 971  FGFGFGFD------DFSIKPIKTGSTISNDFLPPKLSIFADSVPSPPANASDVSPTKPSL 1030

Query: 1032 WGFNAGGSTKTDNDVNRDNYFFDSGDLGLNPIRTEPFQAKRSTFAFDESVPSTPLF--NS 1091
            +  +   +  T+N        F    +   P       A++ +F FD+SVPSTP +  N 
Sbjct: 1031 FADSVPSTPATNNASYPGQKSFFDDSVPSTPAYPGNLFAEKKSF-FDDSVPSTPAYPGNL 1090

Query: 1092 GNSPNNYHEGSEPSFENFSRFD--------SSSVHEGGFPPQDTFARFDSMRSSRDFDQG 1151
                 +Y + S PS   +S  D         +   +  FP +  F  FDS+ S+      
Sbjct: 1091 FAEKKSYFDDSVPSTPAYSTSDFGGKPFASETPRSDNLFPGRSPF-MFDSVPSTPAAHDD 1150

Query: 1152 PGFSPFGQFDTSRSSRDFDQGGSSLTRFDSMRSSKD---FDQGFPSFS--RFDSMR---- 1158
               + F +FD+  S+   +    SL+R DSMRS+ +   F   F SF+  R+DS      
Sbjct: 1151 FSNNSFSRFDSFNSN---NNDAFSLSRTDSMRSTSEPDPFASRFDSFNYQRYDSFNAQSY 1210

BLAST of Cp4.1LG04g04580 vs. TAIR 10
Match: AT1G21630.2 (Calcium-binding EF hand family protein )

HSP 1 Score: 651.4 bits (1679), Expect = 1.4e-186
Identity = 523/1298 (40.29%), Postives = 667/1298 (51.39%), Query Frame = 0

Query: 12   DLFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNA 71
            DLFD YFRRADLD DG ISGAEAVAFFQGS LPK VLAQ+W+ +D ++ G+LGRAEF NA
Sbjct: 11   DLFDTYFRRADLDGDGHISGAEAVAFFQGSNLPKHVLAQVWSYADSKKAGYLGRAEFYNA 70

Query: 72   LRLVTVAQSKRELTPEIVKAALFSPAASKIPAPQINFNTPPASQFNSTPALPTPQTGIGT 131
            L+LVTVAQS+RELT EIVKAA++SPA++ IPAP+IN    P+ Q      LP  Q    T
Sbjct: 71   LKLVTVAQSRRELTAEIVKAAIYSPASANIPAPKINLAATPSPQ--PRGVLPATQAQGVT 130

Query: 132  PTPSQVSGLESQVARSVTTTASP--VPSRENQSVR-PPLATSNSAFHPVQGFSGVGPLSG 191
              PS  +G+         +T++   VP ++NQ    PP  T  +   P     G    + 
Sbjct: 131  SMPSVAAGVRGPHMGGTVSTSNQQVVPGQQNQFTGIPPSQTQQNFQSPGMPAGGT---NA 190

Query: 192  PPPTNSSISNDWVSDRAGGVQGT-----PSQPPNRGF---SPAGTQVGFGQSSAGLITSL 251
            P P N  + +DW+S R+ G  G      PS     G    +     +     +  + +S 
Sbjct: 191  PRPANQPMPSDWLSGRSVGPSGNVNSQIPSSQSTYGLTAPNSTANHITKPHITPAVTSST 250

Query: 252  PPRPQTA-----PGIKSAT--------PS----PVDSNVQGVSGNGIASGSYFGGGHFAA 311
              RPQ +     P   SAT        PS    P D      SGNG  S S F G  F+ 
Sbjct: 251  TTRPQESAPVHNPQESSATFGSRVSNVPSNQLVPKDPKELAASGNGFTSDSLF-GDVFSV 310

Query: 312  SSVPSKQDVPSDKLSTGNISSTSVTVPVPSATQPIVRAGSLDSLQNSFMKPPLANQPPRN 371
            +S   KQ       +TG  S T+ TV  P  TQ +VR  S+   Q S  +  +  Q    
Sbjct: 311  TSTQPKQHPTGSASTTGISSVTTGTVAGPEITQSVVRQSSIPQ-QGSLSQHAVGVQTQLT 370

Query: 372  QPLGKPNQQS--ISHPASSGLSTG-----------------SQNSVSGQSQRPWPRMTQT 431
               G+P   S   S P  S +  G                  Q    GQSQ PWP+MT  
Sbjct: 371  GNSGQPYTSSGAASGPPGSTVGVGISATSQLAQRPPHPHSQPQPRPQGQSQPPWPKMTPA 430

Query: 432  DVQKYTKVFVVVDKDRDGKITGEEARNLFLSWRLPREVLKQVWDLSDQDNDSMLSVREFC 491
            DVQKYTKVFV VD DRDGKITG +ARNLFLSWRLPR+ LKQVWDLSDQDNDSMLS+REFC
Sbjct: 431  DVQKYTKVFVQVDTDRDGKITGNQARNLFLSWRLPRDALKQVWDLSDQDNDSMLSLREFC 490

Query: 492  IALYLLERHREGHILPAMLPSNVVFD---FSSNGHPVTPAASNYSNAAWRPTAGYQQHQG 551
            IA+YL+ER+REG  LP + PS+++     F+S G  V P    + NA+W    G+QQ   
Sbjct: 491  IAVYLMERYREGRPLPPVFPSSIIHSESMFTSPGQSVAP----HGNASWGHPQGFQQQ-- 550

Query: 552  VPGSGNLHGAPVVGERPPTPAAASPVEDEPQTNKPKSK---------------------- 611
             P  G L        +PP P   SP +   Q  +PK K                      
Sbjct: 551  -PHPGGLRPPAGPKGKPPRPVPLSPSDGMVQPTQPKRKMPVLEKPLVDQLSKEEQDSLNT 610

Query: 612  -----------------------------------------VEELEKEILESRQKIEYYR 671
                                                     V+ELEKEI +S+QKI+++R
Sbjct: 611  KFEEATAVDKKFSFFAFSIRRYLLPLPYGIYMKYLWICVAIVDELEKEIADSKQKIDFFR 670

Query: 672  TKMQELVLYKSRCDNRLNEISERVSSEKREVESFAKKYEEKYKQSGDVASRLTVEEATFR 731
             KMQELVLYKSRCDNR NEI+ERV  +KRE+ES AKKYEEKYK+SG+V S+LT+EEATFR
Sbjct: 671  AKMQELVLYKSRCDNRYNEIAERVLGDKRELESLAKKYEEKYKKSGNVGSKLTIEEATFR 730

Query: 732  DIQEKKMELYHAIVKMEQDVSEDGIIQVRADRIQADIEDLVKSLNERCKSYGLRAKPITL 791
            DIQEKKMELY AIVK E+   +D I++ R + IQ+ +E+L+K+LNERCK YG+R KP +L
Sbjct: 731  DIQEKKMELYQAIVKFEEGKLDDSIVK-RTEHIQSGLEELIKNLNERCKQYGVRGKPTSL 790

Query: 792  SELPFGWQPGIQVGAADWDEDWDKFEEEGFTVVKELTLDVQNVIAPPKQKSKSESVQKEQ 851
             ELPFGWQPGIQ GAADWDEDWDK E+EGFT VKELTLD+QNVIAPPK+  KS + +KE 
Sbjct: 791  VELPFGWQPGIQEGAADWDEDWDKLEDEGFTFVKELTLDIQNVIAPPKE--KSSAWRKE- 850

Query: 852  GSAIDSQKVTPAPDVDTKDGD--STPDADSKEEKPPSMDEKAVENGSVHDNKSEDGSARS 911
                         DV +K+G+  S  DADSK  K  S  E   E+    + K+ D  AR 
Sbjct: 851  ------------VDVSSKEGEDVSFSDADSKTGKKQSSGE---EDSEQSEGKTSDVDARD 910

Query: 912  APNSPFASSVIESPKEHRDSNFGKAAGFDASPRDKDTQYRDSNFGKAAGFDASPRDKDTQ 971
               S              DS   K    D+SPR KDT+   S  G   G   +   K   
Sbjct: 911  KNGS------------LDDSKVRKGIEADSSPRTKDTR---SENGHDDGESTASAGKTVN 970

Query: 972  YRDSNLGKAAGFDASP---RDKDTQYRDSNLGKAAGFDTSPRDKDALSDHGGVGSVFSGD 1031
            Y DS+    +    +P   +DKD    DS  G   GFD      D        GS  S D
Sbjct: 971  Y-DSHDETDSVSSVNPDNGKDKDHGKYDSGFGFGFGFD------DFSIKPIKTGSTISND 1030

Query: 1032 ----------KSYDEPAWGTFDMNDDIDSVWGFNAGGSTKTDNDVNRDNYFFDSGDLGLN 1091
                       S   P     D++    S++  +   +  T+N        F    +   
Sbjct: 1031 FLPPKLSIFADSVPSPPANASDVSPTKPSLFADSVPSTPATNNASYPGQKSFFDDSVPST 1090

Query: 1092 PIRTEPFQAKRSTFAFDESVPSTPLF--NSGNSPNNYHEGSEPSFENFSRFD-------- 1151
            P       A++ +F FD+SVPSTP +  N      +Y + S PS   +S  D        
Sbjct: 1091 PAYPGNLFAEKKSF-FDDSVPSTPAYPGNLFAEKKSYFDDSVPSTPAYSTSDFGGKPFAS 1150

Query: 1152 SSSVHEGGFPPQDTFARFDSMRSSRDFDQGPGFSPFGQFDTSRSSRDFDQGGSSLTRFDS 1158
             +   +  FP +  F  FDS+ S+         + F +FD+  S+   +    SL+R DS
Sbjct: 1151 ETPRSDNLFPGRSPF-MFDSVPSTPAAHDDFSNNSFSRFDSFNSN---NNDAFSLSRTDS 1210

BLAST of Cp4.1LG04g04580 vs. TAIR 10
Match: AT4G05520.1 (EPS15 homology domain 2 )

HSP 1 Score: 75.5 bits (184), Expect = 3.2e-13
Identity = 34/81 (41.98%), Postives = 56/81 (69.14%), Query Frame = 0

Query: 13 LFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNAL 72
          ++  +F  AD D DGR+SG +A  FF  S L +Q L Q+WA++D ++ GFLG +EF  A+
Sbjct: 19 IYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAM 78

Query: 73 RLVTVAQSKRELTPEIVKAAL 94
          +LV++AQ   E+T +++K ++
Sbjct: 79 KLVSLAQEGHEITSDLLKGSI 99

BLAST of Cp4.1LG04g04580 vs. TAIR 10
Match: AT4G05520.2 (EPS15 homology domain 2 )

HSP 1 Score: 75.5 bits (184), Expect = 3.2e-13
Identity = 34/81 (41.98%), Postives = 56/81 (69.14%), Query Frame = 0

Query: 13 LFDVYFRRADLDRDGRISGAEAVAFFQGSGLPKQVLAQIWALSDPRQIGFLGRAEFNNAL 72
          ++  +F  AD D DGR+SG +A  FF  S L +Q L Q+WA++D ++ GFLG +EF  A+
Sbjct: 19 IYKEWFNIADSDGDGRVSGNDATKFFAMSKLSRQELKQVWAVADSKRQGFLGLSEFITAM 78

Query: 73 RLVTVAQSKRELTPEIVKAAL 94
          +LV++AQ   E+T +++K ++
Sbjct: 79 KLVSLAQEGHEITSDLLKGSI 99