Cp4.1LG03g17370 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g17370
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	tRNA N6-adenosine threonylcarbamoyltransferase
Location	Cp4.1LG03: 12752493 .. 12757353 (+)
RNA-Seq Expression	Cp4.1LG03g17370
Synteny	Cp4.1LG03g17370

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGTCTCCTTCGACCTTACAGACTTTCAGAAGCGCTGCTGCTCCGCGGGAATGGCATTTTCTTCATCACTTTCCCGCTTAAACCTTATCCCAAAACCTTCACTTCCGTACGCGCTTAATACTTTGAAATCACTGAACTTCCACAAGCTACTACGAATCAATGGGATTCGACCATGTATATCATCAAATGATTCAGCTGCCCGATTTTCTGCTTTGAAAATGACTAGCTCTGCTGGAATCCACACTCAAATTTCAAGGCCGCAAGACGATTTGATTGTTCTTGGCATCGAAACGAGCTGCGATGATACCGCGGCTGCCATTGTAAGCTATAGACCTTGCTTTACTTTGCGTATTTTCGAGCGAGTGCTTTTGACTGTTGTAAACGTGAATTCTGGTTTTTATGATGCTTTTAGGTAAAAGGAAATGGCGAAATTCTTAGCCAAGTTATAGTTTCTCAGGTATTTATCTTTAAGTTAATATCAATGAATCTCCCTTTTCCCAGGAGTTTTTGCACATTTGTAATGTTAGGTTTCATTATACTTGGAACGAGTTTCGAATTTTTTAGTCAAACATGTTGATAATTTTGTCTATTTGGAGTTATTTAGTTATTTCTTGCAGTACATTTTTGTCTAAATTTATCCTTGCCACATCAAGGCAGAGCTGCTTGCTCGTTATGGAGGTGTTGCTCCTAAAATGGCAGAAGAAGCGCATGCACAAGCGATTGACAAGGTCATGATTGCAAGGCATACTAAATGATGAATTTCCATATCTTCTTTATTTACTCTCACGTAGGTTTCAATTCCTTTTTGTCTGTCGACTACAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCGGCAGTTGCTGTAACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGGTAATTTGCAGGATTCCCCCTTAGGGTTTAAGCCTAAGTTATCTGTCTGCTTTGTGCTGTTGCAAACCCATCTGTTTTATCTCTTTATTAGCACTGTTAAACAAATATGTAAGATATATGTATCAATGCTTATGTTCGCACGAATATGGTTCTTAGGCTAGTAGCCATGGAACGAATCACTATTCACTAATCATAACATAGACGTACGGAATCAGTTCAGCTTCTTTCTATCTTTAATTATACAAGTGAAGATTTATCTTTATTAGGAGATTCTTAGGACTTATTCTCCATGCAATTGAAATGCAGTTGGTGTGCAGAAGGCCCGGAAAATTGCTGGTAGCTTTAGTCTACCAATTGTTGGGGTGCATCACATGGAGGCTCATGCTCTGGTGGCTAGGTATGCATATCAAATTATCAATGCAAATTAGATCTGGGTTGGCTCATATTTGTTAATTTGTTTACTGTCAGAGGATGAATTTTTGGTTTGGTTTGGTTTGGTTTGTACTTCCTGGCGGGATACTTTTCTGATTAAAACATTCTTCTTTCCTCTGTAGATTAGTTGAACGGGAGTTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGTAAGACTTTATATTCTCCTATACATTCTCCATTCACATTTATTTTTCATCCTTCCTTCATTTTCCTTACACACATGCAAATTGTATTTTCATTTCCCGTGTCTAGATGTGTAAAAACAGAAAAGAGATAGAGAGTGCAAATTTCTGGATTAATGTATTTTTAAATTGCTTGATCTTTTCCAACTTTTTGTTTCAAAATTTTAGGGTCTTTTATAATTTGATGCATCTCAAATCCAATTATGAAATGCTATGCAGGAGGTAAAACTTCTAAAGTCGTTAACATTTAGGTTCAGTTCTTTTTATTGATAGCATTATATTTAAATGGTCGAGCGCTGTCATTGCTATCTCATTAATTGTAAGTACATTATTTGAACTATTTCAATAAATTTTGAGCAATGGAGTGGGTTGAACTTCAGTTTCATGAATAGAATTGGTGTTTCATGAGTGCGATGCATAAGGGGAAAAGTATTACAAACTACTACATTTTTAATCAACTGTGATGGAGAGAACGAACACTGCATCTTTTTCATGACTGTAGAGGTAAAGTTTGTCTGGTTTATTCTGCTCATTGTGGAGTATAGTATTCGGTCTTCTAGGAGGTGTTATTAAGATGACTCAAGATGATTTTTCGAGCTTTTGACAAGGACTTCAAAGAATAGAAAATTAATGTCTATCCATTCTAGGGGGACACAACTTACTTGTTCTCGCCCAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCGATTGGGGAGGCATATGACAAGACTGCCAAATGGCTTGGTCTTGATCTTAGGAAGAGCGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAATCAATTAAGTTCTCAGTAATTTTTCTAATTCAACGTGTTGTTTGTCATTGCATTTGCATATCCTTATTTTATTGGTAGTGAATAGACATGAGATACAAATAGTAAGCATATATTTCTGTGTTGGCAAGTATGTATATTAAGTTTTCAAATATTTATCTCTATAGACTCCAATGAAGCAACATAAAGATTGCAACTTCTCTTATGCGGGGCTGAAGACTCAAGTCAGGCTGACTATTGAATCCAGAAACATGTATGTATTTATCTGGAATGTACTTCTTCATCCTTTCTAATAACAATGTCATCAGCAAGAATCACATATAATCTTTGAGCTTCAAATAACCTAACCATGTTGTTGGAGTTGTGGCAAAATCTATACAAAAATGAAAATCCAAATATAGTTTTGATGTAAAGGGCCAGACATAGTTAATATTTATTTTGAAGGCATGATGTTTTAAAGGTCTATTAGATTTGAAGTATTGATGTATCTACCCGGTTTTAAGTAACTCATGCTATTAATTTCACAATGTAATATCTGAGCACTTGGGTCATTTGTTTGTATAGTTTATTTCCAGTACTACAGTTCCGTCAGATTGGTAAGACTGGGCTGATGGTTATCCTGTCTGAGAAGCTTATTACAGAGTTTTCACGTGTTGAAAGTTTCTGCTTTGACATTTTTAAGAGCAATAGGTTTTTTTTCTCAGTTCTTTGATGAATGTACATAACGTGTGCAGTGATGCAAAAATTCCTCTTTCTTCTGCAAGCAGCCAAGACAGGAGTGTGAGGGCTGATATTGCTGCCTCTTTTCAGGTATCCATCAGTCTGTATGGACTAATCTTGTACCGTTACGTCAATTGATCCTTGTGTTAAATTTATTGCAGCGTGTTGCAGTACGGCATCTAGAGGAAAGGTGTGAACGAGCAATTGAATGGGCATTGAAGATTGAACCATCCATAAAACATTTGGTAAAATGAGCTGCTCAACATCTTTTATTCCCTTCGCATTACTCCCAATGCATCTTACATTTTCATTTTCTTGCCAGTGAGCTGTACACATTGTTGAAACGAGGAATTCATCTTGTGTTTTTCCTATGCAGGTAGTCTCAGGAGGTGTTGCATCAAATAAGTACGTTAGGGCTCAACTTACTGAGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTATATTTCCTTTTGCTCAACTTGGATAAATTCATGTCTATTAGAGTAATTGACCATTTATGTAACAACCCAAGCTCACCACCAACAAATATTGTCATTTTTAGGCTTTTCCTTTCAGGCTTTCCCTCAAAGTTTTTAAAACGTGTTTGCTAGGGAGTGGTTTCCACACCTTTATAAAGAATGATTTGTTCCCCTCTCCAACAGATATGAGATCTCTAAATTTAAAACCTGAACTCGGACATAAAAAATGTAGGTTGACCATAGTTTAAAAATGATTAATGTTGATGACTTATTTTAAGCTCCAAGTACGTTTGTCGGTGTGTTTATGGGAGATGTAGAGAGCTTTCCTTAACAAAGTAGCAGATTTTCTTGTGCAAAATATTTGGCCTGCTTAATATACAACGGTTTTGTTGACCATTTCTGGTGCAATCTTATTTAAATAAGTTCGCACACCGTATCGTATGCGTATTAGGACACCAATCTCTTTTAATTGTGTTGCCAAGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTATCGCATTGGGAGATTTGATCCTCCACCACCAGCCGATGAACCCGAAGATACTTTGGTATGGTTCTAATCTTCACTGTAACCCTCTTGCCCCTTGATTAAAATAGGAACAAGTGAAGCCATCACATGTTTGGGTTTTACTACTAAGCTTAGAAAAAGAATACTTTGTCCAAAGTTATTCAAAGGGCCTGATGAATATTCTAGTTTATGATGACAGCATCTTTCTTTTATTTTATGGTTTTTTATCCTTCTCAAGTCTACATATTCAATTTTGTATCCAGTACGACGTGCGACCGAGGTGGCCATTGGGAGAAGAATACGTTGAGGGAAGGAGTATAGCTCGCTCGCTAAGAACAGCCCGAATCCATCCATCTCTTACGTCTATCATCCAGGCATCTCTACAGCAACAATAGTAGCCATAGCTTCAGTTTCCAACTCAAAGGAATTTGCATCTCTTCTTACCTACGAGATTTCTCAGGAAACAAACGGAATCATACCGGAGTTCTAATTTCCCAACCATCAACGTGGTACTACCATCACTTGATGCCTTCAGTTAAATTATTGGGCGAGTTTGGTTGTTTTCATGAGATATGACATCAATATGTTATGTTATTTATTGATTCATTTGTATCATATTAACATATATTAACAAGTCTCATACACGAGAACCTTCAAAAGAAGAAATACATGTGGATCATCGCTAAGTTATGCTCAGATAGTCAATTATTAGGTAATCTT

mRNA sequence

CGTCTCCTTCGACCTTACAGACTTTCAGAAGCGCTGCTGCTCCGCGGGAATGGCATTTTCTTCATCACTTTCCCGCTTAAACCTTATCCCAAAACCTTCACTTCCGTACGCGCTTAATACTTTGAAATCACTGAACTTCCACAAGCTACTACGAATCAATGGGATTCGACCATGTATATCATCAAATGATTCAGCTGCCCGATTTTCTGCTTTGAAAATGACTAGCTCTGCTGGAATCCACACTCAAATTTCAAGGCCGCAAGACGATTTGATTGTTCTTGGCATCGAAACGAGCTGCGATGATACCGCGGCTGCCATTGTAAAAGGAAATGGCGAAATTCTTAGCCAAGTTATAGTTTCTCAGGCAGAGCTGCTTGCTCGTTATGGAGGTGTTGCTCCTAAAATGGCAGAAGAAGCGCATGCACAAGCGATTGACAAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCGGCAGTTGCTGTAACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGTGTGCAGAAGGCCCGGAAAATTGCTGGTAGCTTTAGTCTACCAATTGTTGGGGTGCATCACATGGAGGCTCATGCTCTGGTGGCTAGATTAGTTGAACGGGAGTTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAACTTACTTGTTCTCGCCCAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCGATTGGGGAGGCATATGACAAGACTGCCAAATGGCTTGGTCTTGATCTTAGGAAGAGCGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAATCAATTAAGTTCTCAACTCCAATGAAGCAACATAAAGATTGCAACTTCTCTTATGCGGGGCTGAAGACTCAAGTCAGGCTGACTATTGAATCCAGAAACATTGATGCAAAAATTCCTCTTTCTTCTGCAAGCAGCCAAGACAGGAGTGTGAGGGCTGATATTGCTGCCTCTTTTCAGCGTGTTGCAGTACGGCATCTAGAGGAAAGGTGTGAACGAGCAATTGAATGGGCATTGAAGATTGAACCATCCATAAAACATTTGGTAGTCTCAGGAGGTGTTGCATCAAATAAGTACGTTAGGGCTCAACTTACTGAGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTATCGCATTGGGAGATTTGATCCTCCACCACCAGCCGATGAACCCGAAGATACTTTGTACGACGTGCGACCGAGGTGGCCATTGGGAGAAGAATACGTTGAGGGAAGGAGTATAGCTCGCTCGCTAAGAACAGCCCGAATCCATCCATCTCTTACGTCTATCATCCAGGCATCTCTACAGCAACAATAGTAGCCATAGCTTCAGTTTCCAACTCAAAGGAATTTGCATCTCTTCTTACCTACGAGATTTCTCAGGAAACAAACGGAATCATACCGGAGTTCTAATTTCCCAACCATCAACGTGGTACTACCATCACTTGATGCCTTCAGTTAAATTATTGGGCGAGTTTGGTTGTTTTCATGAGATATGACATCAATATGTTATGTTATTTATTGATTCATTTGTATCATATTAACATATATTAACAAGTCTCATACACGAGAACCTTCAAAAGAAGAAATACATGTGGATCATCGCTAAGTTATGCTCAGATAGTCAATTATTAGGTAATCTT

Coding sequence (CDS)

ATGGCATTTTCTTCATCACTTTCCCGCTTAAACCTTATCCCAAAACCTTCACTTCCGTACGCGCTTAATACTTTGAAATCACTGAACTTCCACAAGCTACTACGAATCAATGGGATTCGACCATGTATATCATCAAATGATTCAGCTGCCCGATTTTCTGCTTTGAAAATGACTAGCTCTGCTGGAATCCACACTCAAATTTCAAGGCCGCAAGACGATTTGATTGTTCTTGGCATCGAAACGAGCTGCGATGATACCGCGGCTGCCATTGTAAAAGGAAATGGCGAAATTCTTAGCCAAGTTATAGTTTCTCAGGCAGAGCTGCTTGCTCGTTATGGAGGTGTTGCTCCTAAAATGGCAGAAGAAGCGCATGCACAAGCGATTGACAAGGTGGTGCAGGAAACAATGGAAAAGGCTAACTTAACAGAAAGGGATTTATCGGCAGTTGCTGTAACCATTGGCCCTGGTTTGAGCCTCTGCCTCCGTGTTGGTGTGCAGAAGGCCCGGAAAATTGCTGGTAGCTTTAGTCTACCAATTGTTGGGGTGCATCACATGGAGGCTCATGCTCTGGTGGCTAGATTAGTTGAACGGGAGTTGCAATTTCCTTTCATGGCACTGCTCATTTCTGGGGGACACAACTTACTTGTTCTCGCCCAAGATCTTGGCCAATACATACAACTTGGAACAACAATAGACGATGCGATTGGGGAGGCATATGACAAGACTGCCAAATGGCTTGGTCTTGATCTTAGGAAGAGCGGTGGGCCGGCTATAGAGGAGCTTGCCAGAGAGGGGGATCCAAAATCAATTAAGTTCTCAACTCCAATGAAGCAACATAAAGATTGCAACTTCTCTTATGCGGGGCTGAAGACTCAAGTCAGGCTGACTATTGAATCCAGAAACATTGATGCAAAAATTCCTCTTTCTTCTGCAAGCAGCCAAGACAGGAGTGTGAGGGCTGATATTGCTGCCTCTTTTCAGCGTGTTGCAGTACGGCATCTAGAGGAAAGGTGTGAACGAGCAATTGAATGGGCATTGAAGATTGAACCATCCATAAAACATTTGGTAGTCTCAGGAGGTGTTGCATCAAATAAGTACGTTAGGGCTCAACTTACTGAGGTTGTCAGGAAGAAGGGCCTGCAGCTTGTCTGCCCTCCTCCCAGTCTCTGCACTGATAATGGTGTAATGGTGGCTTGGACGGGCCTCGAGCATTATCGCATTGGGAGATTTGATCCTCCACCACCAGCCGATGAACCCGAAGATACTTTGTACGACGTGCGACCGAGGTGGCCATTGGGAGAAGAATACGTTGAGGGAAGGAGTATAGCTCGCTCGCTAAGAACAGCCCGAATCCATCCATCTCTTACGTCTATCATCCAGGCATCTCTACAGCAACAATAG

Protein sequence

MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSSAGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGGVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPEDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ

Homology

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot
Match: O22145 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GCP1 PE=2 SV=2)

HSP 1 Score: 665.6 bits (1716), Expect = 4.1e-190
Identity = 339/472 (71.82%), Postives = 397/472 (84.11%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPS-LPYALNTLKSLNFHKLL----RINGIRPCISSNDSAARFSAL 60
           +  S ++SR NL P  S L    N+L+    HKL       + I P  S N    RF + 
Sbjct: 6   LTLSPAISRFNLYPGISILARNNNSLRLQKHHKLKTKTPTFSLISPSSSPNFQRTRFYST 65

Query: 61  KMTSSAGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGV 120
           +   S+  +++     D+L+VLGIETSCDDTAAA+V+GNGEILSQVI SQAELL +YGGV
Sbjct: 66  ETRISSLPYSENPNFDDNLVVLGIETSCDDTAAAVVRGNGEILSQVISSQAELLVQYGGV 125

Query: 121 APKMAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSF 180
           APK AEEAH++ IDKVVQ+ ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+F
Sbjct: 126 APKQAEEAHSRVIDKVVQDALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNF 185

Query: 181 SLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAI 240
           SLPIVGVHHMEAHALVARLVE+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAI
Sbjct: 186 SLPIVGVHHMEAHALVARLVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAI 245

Query: 241 GEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRL 300
           GEA+DKTAKWLGLD+ +SGGPA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRL
Sbjct: 246 GEAFDKTAKWLGLDMHRSGGPAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRL 305

Query: 301 TIESRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHL 360
            IE++ IDAK P+SSA+++DR  RADIAASFQRVAV HLEE+CERAI+WAL++EPSIKH+
Sbjct: 306 AIEAKEIDAKCPVSSATNEDRRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHM 365

Query: 361 VVSGGVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPP 420
           V+SGGVASNKYVR +L  +V  K L+LVCPPPSLCTDNGVMVAWTGLEH+R+GR+DPPPP
Sbjct: 366 VISGGVASNKYVRLRLNNIVENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPP 425

Query: 421 ADEPEDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQA-SLQQQ 467
           A EPED +YD+RPRWPLGEEY +GRS ARS+RTARIHPSLTSII+A SLQQQ
Sbjct: 426 ATEPEDYVYDLRPRWPLGEEYAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot
Match: B3PQA4 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 652) OX=491916 GN=tsaD PE=3 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.0e-72
Identity = 160/364 (43.96%), Postives = 223/364 (61.26%), Query Frame = 0


Query: 76  VLGIETSCDDTAAAIV----KGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKV 135
           +LGIETSCD+TAAA+V    +G+  ILS V++SQ +  + YGGV P++A  AH +A+D++
Sbjct: 7   ILGIETSCDETAAAVVERDAEGHSRILSDVVLSQLDEHSAYGGVVPEIAARAHVEALDEL 66

Query: 136 VQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALV 195
           ++E + +AN++  D+ A+A T GPGL   L VG+   + IA +   P+  V+H+E HAL 
Sbjct: 67  IEEALMRANVSLADVDAIAATSGPGLIGGLLVGLMTGKAIAKAAGKPLYAVNHLEGHALT 126

Query: 196 ARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLR 255
           ARL +  L FP++ LL+SGGH  L+L + +G Y + GTTIDDA+GEA+DKTAK LGL   
Sbjct: 127 ARLTD-GLSFPYLMLLVSGGHTQLILVRGVGDYERWGTTIDDALGEAFDKTAKLLGLPY- 186

Query: 256 KSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLSSA 315
             GGPA+E +AR+G+     F  P+      +FS++GLKT VRL  +         ++  
Sbjct: 187 -PGGPAVERMARDGNAGRFDFPRPLVGEARLDFSFSGLKTAVRLAAQE--------IAPL 246

Query: 316 SSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKH---LVVSGGVASNKYVR 375
           S QD    ADI ASFQR   R L++R  R ++   +  PSI     LVV+GGVA+N  +R
Sbjct: 247 SDQD---VADICASFQRAISRTLKDRIGRGLQRFKREFPSIGETPALVVAGGVAANLELR 306

Query: 376 AQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPEDTLYDVRP 433
           A L  +  K G + + PP SLCTDN VM+AW GLE   IG          P+      R 
Sbjct: 307 ATLQGLCDKNGFRFIAPPLSLCTDNAVMIAWAGLERMAIG--------VAPDALDVQPRS 348

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot
Match: Q0P4K0 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopus tropicalis OX=8364 GN=osgepl1 PE=2 SV=2)

HSP 1 Score: 274.2 bits (700), Expect = 2.6e-72
Identity = 147/342 (42.98%), Postives = 205/342 (59.94%), Query Frame = 0


Query: 75  IVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQE 134
           IVLGIETSCDDT AA+V  NG IL + +  Q ++  + GG+ P +A+  H   I KVV +
Sbjct: 29  IVLGIETSCDDTGAAVVDENGTILGEALHCQKDIHLKTGGIIPTVAQHLHRDNITKVVNK 88

Query: 135 TMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVARL 194
            +  + ++  +LSA+A T+ PGL LCL VG+  +  +   +  P + +HHMEAHAL  RL
Sbjct: 89  AIHASGISPYELSAIATTVKPGLGLCLGVGLSYSLDLVNKYHKPFIPIHHMEAHALTVRL 148

Query: 195 VERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLR--- 254
           +   ++FPF+ LLISGGH +L +   + +++ LG ++D+A G+  DK A+ L L      
Sbjct: 149 L-HPVEFPFLVLLISGGHCILAIVSGVSEFVMLGHSLDEAPGDTLDKVARRLSLINHPQC 208

Query: 255 --KSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLS 314
              SGG AIE LA  G+ K  K   PM  H+DCNFS+AGL+ QV   IE +  +  I   
Sbjct: 209 SGMSGGEAIEHLALHGNRKICKLKIPMSHHRDCNFSFAGLRNQVNKVIEQKEAEKGI--- 268

Query: 315 SASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALK---IEPSIKHLVVSGGVASNKY 374
            +  Q     ADIAA+ Q     HL +R +RAI +  +   I      LVVSGGVASN+Y
Sbjct: 269 -SKGQLLPCVADIAAAVQHTVALHLAQRTQRAIYYCKREGLIPTERACLVVSGGVASNRY 328

Query: 375 VRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIG 409
           +R  L  V  +  + L+CPPP LCTDNGVM+AW G+E  + G
Sbjct: 329 IRKALQTVTHESDMTLLCPPPRLCTDNGVMIAWNGIEKLQSG 365

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot
Match: Q0BPC9 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) OX=391165 GN=tsaD PE=3 SV=2)

HSP 1 Score: 273.9 bits (699), Expect = 3.4e-72
Identity = 159/365 (43.56%), Postives = 220/365 (60.27%), Query Frame = 0


Query: 76  VLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQET 135
           VLGIETSCD+TAAA++ G+G IL+++++SQ +  AR+GGV P++A  AH   +  +V E 
Sbjct: 14  VLGIETSCDETAAAVLDGSGRILAEIVLSQYDDHARFGGVVPEIAARAHLAYLPGMVTEV 73

Query: 136 MEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVARL- 195
           M+KA L  +DL+A+A T GPGL   L VG    + +A +   P + ++H+EAHAL A L 
Sbjct: 74  MDKAGLRFQDLAAIAATSGPGLIGGLLVGAGLGKGLALAAKRPFIAINHLEAHALAALLP 133

Query: 196 -------VERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLG 255
                  +     FPF+ +L+SGGH   +L + +G+Y +LG TIDDA+GEA+DK  K LG
Sbjct: 134 ALGGVAEITSGEHFPFLLMLLSGGHCQCILVEGVGRYRRLGGTIDDAVGEAFDKVGKLLG 193

Query: 256 LDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIP 315
           L     GGPA+E LA +G+P ++ F  PMK    C+FS++GLKT V   + +R  D  +P
Sbjct: 194 LGW--PGGPALERLALQGNPHALAFPRPMKGRVGCDFSFSGLKTAVAQYV-ARFPDGPLP 253

Query: 316 LSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGGVASNKYV 375
           LS A        ADIAASFQ      + +R   A+  A +I P+ K LVVSGGVA+N  +
Sbjct: 254 LSDA--------ADIAASFQAAVADVMADRATAALAMADEIAPA-KMLVVSGGVAANAAI 313

Query: 376 RAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPEDTLYDVR 433
           RA L+     +G+ ++ PPP LCTDN VMVAW GL   + G       A           
Sbjct: 314 RAALSTAAEHRGIAMLAPPPRLCTDNAVMVAWAGLHRLKYGAVSGLDHAP---------L 357

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot
Match: Q2VYV2 (tRNA N6-adenosine threonylcarbamoyltransferase OS=Magnetospirillum magneticum (strain AMB-1 / ATCC 700264) OX=342108 GN=tsaD PE=3 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 5.9e-72
Identity = 154/359 (42.90%), Postives = 211/359 (58.77%), Query Frame = 0


Query: 74  LIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQ 133
           ++VLGIE+SCD+TAAA+V    EIL +V++SQ +    +GGV P++A  +H   +DK+V 
Sbjct: 1   MLVLGIESSCDETAAAVVNDRREILGEVVLSQLDEHRPFGGVVPEIAARSHLAHMDKLVA 60

Query: 134 ETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVAR 193
           E M +A +   DL AVA T GPGL   + VGV   + IA +   P + V+H+E HAL  R
Sbjct: 61  EAMRRAGVGFADLDAVAATGGPGLIGGVIVGVMTGKAIALAAGKPFLAVNHLEGHALTPR 120

Query: 194 LVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKS 253
           L   ++ FP++ LL SGGH  L+  + +G+Y +LGTTIDDA GEA+DK AK  GL     
Sbjct: 121 LT-HDIAFPYLLLLASGGHCQLLAVEGVGRYTRLGTTIDDAAGEAFDKVAKMAGLGY--P 180

Query: 254 GGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLSSASS 313
           GGPA+E  A+ GDP       PMK    C+FS++GLK   RL IES       PLS+A  
Sbjct: 181 GGPAVEAAAQGGDPARFTLPRPMKGKPGCDFSFSGLKNAARLLIES----LPQPLSAAD- 240

Query: 314 QDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGGVASNKYVRAQLTE 373
                +AD+A +FQ      + +R  R +       P  +HLVV+GGVA+N  +R  L  
Sbjct: 241 -----QADVARAFQDAVADAMADRVRRGVREMKSRWPQTQHLVVAGGVAANTALRQVLVR 300

Query: 374 VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPEDTLYDVRPRWPL 433
           +  + GL+ + PP  LCTDN  M+AW GLE  R+G+ D         D  +  RPRWPL
Sbjct: 301 IGNETGLEFLAPPLKLCTDNAAMIAWAGLERLRLGQSD---------DLTFAPRPRWPL 337

[Close]

BLAST of Cp4.1LG03g17370 vs. NCBI nr
Match: XP_023527336.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 466/466 (100.00%), Postives = 466/466 (100.00%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466

[Close]

BLAST of Cp4.1LG03g17370 vs. NCBI nr
Match: XP_022924665.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 460/466 (98.71%), Postives = 463/466 (99.36%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRP ISSN SAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPGISSNASAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVR+QLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DTLYDVRPRWPLGEEYVEGRS+ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 DTLYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQQ 466

[Close]

BLAST of Cp4.1LG03g17370 vs. NCBI nr
Match: XP_022980286.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 455/466 (97.64%), Postives = 463/466 (99.36%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRP +SSNDSAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPGLSSNDSAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPK+A
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKIA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARK+AGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKMAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NID KIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDGKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVR+QLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DT+YDVRPRWPLGEEYVEGRS+ARSLRTARIHPSLTSIIQASLQQ+
Sbjct: 421 DTMYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQR 466

[Close]

BLAST of Cp4.1LG03g17370 vs. NCBI nr
Match: KAG6582553.1 (putative tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 457/466 (98.07%), Postives = 462/466 (99.14%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNL+PKPSLPYALNTLKSLNFHKLLRINGIRP ISSN SAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLLPKPSLPYALNTLKSLNFHKLLRINGIRPGISSNASAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEER ERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERSERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVR+QLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DTLYDVRPRWPLGEEYVEGRS+ARSLRTARIHPSLTSIIQASL+QQ
Sbjct: 421 DTLYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLRQQ 466

[Close]

BLAST of Cp4.1LG03g17370 vs. NCBI nr
Match: XP_038877779.1 (probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Benincasa hispida])

HSP 1 Score: 857 bits (2214), Expect = 2.04e-312
Identity = 435/466 (93.35%), Postives = 449/466 (96.35%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLK+LNF KLLRIN +   +S NDSA RFSALK TSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKALNFRKLLRINWVGTGLSLNDSATRFSALKTTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AG+HTQIS+PQDDLIVLGIETSCDDTAAAIVKGNGEILSQVI SQAELLARYGGVAPKMA
Sbjct: 61  AGLHTQISKPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIASQAELLARYGGVAPKMA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLPIV
Sbjct: 121 EEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NIDAKIPLSSAS QDRSVRADIAASFQR+AV HLEERCERAI+WALKIEPSI+HLVVSGG
Sbjct: 301 NIDAKIPLSSASIQDRSVRADIAASFQRIAVLHLEERCERAIDWALKIEPSIRHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVRAQLT VVRKKGLQLVCPPPSLCTDNGVMVAWTG+EH+R+GRFDPPPPADEPE
Sbjct: 361 VASNKYVRAQLTAVVRKKGLQLVCPPPSLCTDNGVMVAWTGIEHFRVGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           D +YDVRPRWPLGEEY EGRS+ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 DIMYDVRPRWPLGEEYTEGRSVARSLRTARIHPSLTSIIQASLQQQ 466

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL
Match: A0A6J1EFN5 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111432100 PE=3 SV=1)

HSP 1 Score: 899 bits (2322), Expect = 0.0
Identity = 460/466 (98.71%), Postives = 463/466 (99.36%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRP ISSN SAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPGISSNASAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVR+QLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DTLYDVRPRWPLGEEYVEGRS+ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 DTLYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQQ 466

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL
Match: A0A6J1IT72 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111479704 PE=3 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 455/466 (97.64%), Postives = 463/466 (99.36%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRP +SSNDSAARFSALKMTSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPGLSSNDSAARFSALKMTSS 60

Query: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMA 120
           AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPK+A
Sbjct: 61  AGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKIA 120

Query: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIV 180
           EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARK+AGSFSLPIV
Sbjct: 121 EEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKMAGSFSLPIV 180

Query: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240
           GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD
Sbjct: 181 GVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYD 240

Query: 241 KTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESR 300
           KTAKWLGLDLRKSGGPAIEELAREGDP+SIKFSTPMKQHKDCNFSYAGLKTQVRL IESR
Sbjct: 241 KTAKWLGLDLRKSGGPAIEELAREGDPESIKFSTPMKQHKDCNFSYAGLKTQVRLAIESR 300

Query: 301 NIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360
           NID KIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG
Sbjct: 301 NIDGKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGG 360

Query: 361 VASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420
           VASNKYVR+QLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE
Sbjct: 361 VASNKYVRSQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEPE 420

Query: 421 DTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           DT+YDVRPRWPLGEEYVEGRS+ARSLRTARIHPSLTSIIQASLQQ+
Sbjct: 421 DTMYDVRPRWPLGEEYVEGRSVARSLRTARIHPSLTSIIQASLQQR 466

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL
Match: A0A0A0L938 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumis sativus OX=3659 GN=GCP1 PE=3 SV=1)

HSP 1 Score: 844 bits (2181), Expect = 2.16e-307
Identity = 434/467 (92.93%), Postives = 444/467 (95.07%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSS+LSRLNLIPKPSLPYAL T K L+FHKLLR + I P +SSN S  RFSALK+TSS
Sbjct: 19  MAFSSTLSRLNLIPKPSLPYALTTFKVLHFHKLLRTDWIGPGLSSNASTPRFSALKITSS 78

Query: 61  AGIHTQISRPQDD-LIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKM 120
           AG HTQISRPQDD LIVLGIETSCDDTAAAIVKGNGEILSQVI SQAELLARYGGVAPKM
Sbjct: 79  AGFHTQISRPQDDDLIVLGIETSCDDTAAAIVKGNGEILSQVIASQAELLARYGGVAPKM 138

Query: 121 AEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPI 180
           AEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLPI
Sbjct: 139 AEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPI 198

Query: 181 VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 240
           VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY
Sbjct: 199 VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 258

Query: 241 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIES 300
           DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRL IES
Sbjct: 259 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIES 318

Query: 301 RNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSG 360
           RNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVSG
Sbjct: 319 RNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSG 378

Query: 361 GVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEP 420
           GVASNKYVRAQL  VVRKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADEP
Sbjct: 379 GVASNKYVRAQLMAVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEP 438

Query: 421 EDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           EDT+YDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 439 EDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQ 485

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL
Match: A0A1S3AWD3 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103483365 PE=3 SV=1)

HSP 1 Score: 839 bits (2168), Expect = 9.34e-306
Identity = 433/467 (92.72%), Postives = 443/467 (94.86%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNFHKLLRINGIRPCISSNDSAARFSALKMTSS 60
           MAFSSSLSRLNLIPKPSLPYAL   K+L+FHKLLR + I P +SSN S  RFSALK TSS
Sbjct: 1   MAFSSSLSRLNLIPKPSLPYAL---KALHFHKLLRAHWIGPALSSNASTPRFSALKTTSS 60

Query: 61  AGIHTQISRPQDD-LIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKM 120
           AG HT+ISRPQDD LIVLGIETSCDDTAAAIVKGNGEILSQVI SQAELLARYGGVAPKM
Sbjct: 61  AGFHTRISRPQDDDLIVLGIETSCDDTAAAIVKGNGEILSQVIASQAELLARYGGVAPKM 120

Query: 121 AEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPI 180
           AEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGS SLPI
Sbjct: 121 AEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSSSLPI 180

Query: 181 VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 240
           VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY
Sbjct: 181 VGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAY 240

Query: 241 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIES 300
           DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRL IES
Sbjct: 241 DKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLAIES 300

Query: 301 RNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSG 360
           RNID KIPLSSAS QDRSVRADIAASFQRVAV HLE++CERAIEWALKIEPSI+HLVVSG
Sbjct: 301 RNIDTKIPLSSASIQDRSVRADIAASFQRVAVLHLEDKCERAIEWALKIEPSIRHLVVSG 360

Query: 361 GVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADEP 420
           GVASNKYVRAQLT V RKKGLQLVCPPPSLCTDNGVMVAWTGLEH+RIGRFDPPPPADEP
Sbjct: 361 GVASNKYVRAQLTAVARKKGLQLVCPPPSLCTDNGVMVAWTGLEHFRIGRFDPPPPADEP 420

Query: 421 EDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           EDT+YDVRPRWPLGEEY EGRS ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 EDTMYDVRPRWPLGEEYTEGRSAARSLRTARIHPSLTSIIQASLQQQ 464

[Close]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL
Match: A0A6J1D0B8 (Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111016118 PE=3 SV=1)

HSP 1 Score: 834 bits (2154), Expect = 1.47e-303
Identity = 420/468 (89.74%), Postives = 443/468 (94.66%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPSLPYALNTLKSLNF--HKLLRINGIRPCISSNDSAARFSALKMT 60
           MAFSSS SRLNLIPKPSLPYA+ +L++LN   HKLLR   + P +S N S+ARFS  K+ 
Sbjct: 1   MAFSSSFSRLNLIPKPSLPYAMTSLRALNLNLHKLLRTKWMEPGMSMNGSSARFSVSKIM 60

Query: 61  SSAGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPK 120
           SSAG+HTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQV+ SQAELLARYGGVAPK
Sbjct: 61  SSAGLHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVVASQAELLARYGGVAPK 120

Query: 121 MAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLP 180
           MAEEAHAQ IDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKAR+IAGS+SLP
Sbjct: 121 MAEEAHAQVIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARRIAGSYSLP 180

Query: 181 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 240
           IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA
Sbjct: 181 IVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEA 240

Query: 241 YDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIE 300
           YDKTAKWLGLDLR+SGGPAIEELAREGDP+S KFSTPMKQHKDCNFSYAGLKTQVRL IE
Sbjct: 241 YDKTAKWLGLDLRRSGGPAIEELAREGDPQSFKFSTPMKQHKDCNFSYAGLKTQVRLAIE 300

Query: 301 SRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVS 360
           SRNIDAK+P+SS SSQDRS+RADIAASFQRVAV HLEERCERAIEWALKIEPSI+HLVVS
Sbjct: 301 SRNIDAKVPISSESSQDRSLRADIAASFQRVAVLHLEERCERAIEWALKIEPSIRHLVVS 360

Query: 361 GGVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPPADE 420
           GGVASNKY+R +LTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHY +GRFDPPPP DE
Sbjct: 361 GGVASNKYIRTRLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYCVGRFDPPPPVDE 420

Query: 421 PEDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQASLQQQ 466
           PEDT+YDVRPRWPLGEEY EGRS+ARSLRTARIHPSLTSIIQASLQQQ
Sbjct: 421 PEDTMYDVRPRWPLGEEYTEGRSVARSLRTARIHPSLTSIIQASLQQQ 468

[Close]

BLAST of Cp4.1LG03g17370 vs. TAIR 10
Match: AT2G45270.1 (glycoprotease 1 )

HSP 1 Score: 665.6 bits (1716), Expect = 2.9e-191
Identity = 339/472 (71.82%), Postives = 397/472 (84.11%), Query Frame = 0


Query: 1   MAFSSSLSRLNLIPKPS-LPYALNTLKSLNFHKLL----RINGIRPCISSNDSAARFSAL 60
           +  S ++SR NL P  S L    N+L+    HKL       + I P  S N    RF + 
Sbjct: 6   LTLSPAISRFNLYPGISILARNNNSLRLQKHHKLKTKTPTFSLISPSSSPNFQRTRFYST 65

Query: 61  KMTSSAGIHTQISRPQDDLIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGV 120
           +   S+  +++     D+L+VLGIETSCDDTAAA+V+GNGEILSQVI SQAELL +YGGV
Sbjct: 66  ETRISSLPYSENPNFDDNLVVLGIETSCDDTAAAVVRGNGEILSQVISSQAELLVQYGGV 125

Query: 121 APKMAEEAHAQAIDKVVQETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSF 180
           APK AEEAH++ IDKVVQ+ ++KANLTE+DLSAVAVTIGPGLSLCLRVGV+KAR++AG+F
Sbjct: 126 APKQAEEAHSRVIDKVVQDALDKANLTEKDLSAVAVTIGPGLSLCLRVGVRKARRVAGNF 185

Query: 181 SLPIVGVHHMEAHALVARLVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAI 240
           SLPIVGVHHMEAHALVARLVE+EL FPFMALLISGGHNLLVLA  LGQY QLGTT+DDAI
Sbjct: 186 SLPIVGVHHMEAHALVARLVEQELSFPFMALLISGGHNLLVLAHKLGQYTQLGTTVDDAI 245

Query: 241 GEAYDKTAKWLGLDLRKSGGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRL 300
           GEA+DKTAKWLGLD+ +SGGPA+EELA EGD KS+KF+ PMK HKDCNFSYAGLKTQVRL
Sbjct: 246 GEAFDKTAKWLGLDMHRSGGPAVEELALEGDAKSVKFNVPMKYHKDCNFSYAGLKTQVRL 305

Query: 301 TIESRNIDAKIPLSSASSQDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHL 360
            IE++ IDAK P+SSA+++DR  RADIAASFQRVAV HLEE+CERAI+WAL++EPSIKH+
Sbjct: 306 AIEAKEIDAKCPVSSATNEDRRNRADIAASFQRVAVLHLEEKCERAIDWALELEPSIKHM 365

Query: 361 VVSGGVASNKYVRAQLTEVVRKKGLQLVCPPPSLCTDNGVMVAWTGLEHYRIGRFDPPPP 420
           V+SGGVASNKYVR +L  +V  K L+LVCPPPSLCTDNGVMVAWTGLEH+R+GR+DPPPP
Sbjct: 366 VISGGVASNKYVRLRLNNIVENKNLKLVCPPPSLCTDNGVMVAWTGLEHFRVGRYDPPPP 425

Query: 421 ADEPEDTLYDVRPRWPLGEEYVEGRSIARSLRTARIHPSLTSIIQA-SLQQQ 467
           A EPED +YD+RPRWPLGEEY +GRS ARS+RTARIHPSLTSII+A SLQQQ
Sbjct: 426 ATEPEDYVYDLRPRWPLGEEYAKGRSEARSMRTARIHPSLTSIIRADSLQQQ 477

[Close]

BLAST of Cp4.1LG03g17370 vs. TAIR 10
Match: AT4G22720.1 (Actin-like ATPase superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-22
Identity = 89/329 (27.05%), Postives = 147/329 (44.68%), Query Frame = 0


Query: 74  LIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQ 133
           +I +G E S +     IV  +G IL+        +     G  P+     H   +  +V+
Sbjct: 5   MIAIGFEGSANKIGVGIVTLDGTILAN--PRHTYITPPGHGFLPRETAHHHLDHVLPLVK 64

Query: 134 ETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVAR 193
             +E + +T  ++  +  T GPG+   L+V     R ++  +  PIV V+H  AH  + R
Sbjct: 65  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 124

Query: 194 LVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKS 253
           +V      P + L +SGG N  V+A   G+Y   G TID A+G   D+ A+ L L    S
Sbjct: 125 VV-TGADDP-VVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPS 184

Query: 254 GGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLSSASS 313
            G  IE+LA++G+     F       K  + S++G+ + +  T E +             
Sbjct: 185 PGYNIEQLAKKGE----NFIDLPYAVKGMDVSFSGILSYIETTAEEK-----------LK 244

Query: 314 QDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGGVASNKYVRAQLTE 373
            +    AD+  S Q      L E  ERA+    K     K +++ GGV  N+ ++  +  
Sbjct: 245 NNECTPADLCYSLQETVFAMLVEITERAMAHCDK-----KDVLIVGGVGCNERLQEMMRT 304

Query: 374 VVRKKGLQLVCPPPSLCTDNGVMVAWTGL 403
           +  ++  +L       C DNG M+A+TGL
Sbjct: 305 MCSERDGKLFATDDRYCIDNGAMIAYTGL 308

[Close]

BLAST of Cp4.1LG03g17370 vs. TAIR 10
Match: AT4G22720.2 (Actin-like ATPase superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-22
Identity = 89/329 (27.05%), Postives = 147/329 (44.68%), Query Frame = 0


Query: 74  LIVLGIETSCDDTAAAIVKGNGEILSQVIVSQAELLARYGGVAPKMAEEAHAQAIDKVVQ 133
           +I +G E S +     IV  +G IL+        +     G  P+     H   +  +V+
Sbjct: 5   MIAIGFEGSANKIGVGIVTLDGTILAN--PRHTYITPPGHGFLPRETAHHHLDHVLPLVK 64

Query: 134 ETMEKANLTERDLSAVAVTIGPGLSLCLRVGVQKARKIAGSFSLPIVGVHHMEAHALVAR 193
             +E + +T  ++  +  T GPG+   L+V     R ++  +  PIV V+H  AH  + R
Sbjct: 65  SALETSQVTPEEIDCICYTKGPGMGAPLQVSAIVVRVLSQLWKKPIVAVNHCVAHIEMGR 124

Query: 194 LVERELQFPFMALLISGGHNLLVLAQDLGQYIQLGTTIDDAIGEAYDKTAKWLGLDLRKS 253
           +V      P + L +SGG N  V+A   G+Y   G TID A+G   D+ A+ L L    S
Sbjct: 125 VV-TGADDP-VVLYVSGG-NTQVIAYSEGRYRIFGETIDIAVGNCLDRFARVLKLSNDPS 184

Query: 254 GGPAIEELAREGDPKSIKFSTPMKQHKDCNFSYAGLKTQVRLTIESRNIDAKIPLSSASS 313
            G  IE+LA++G+     F       K  + S++G+ + +  T E +             
Sbjct: 185 PGYNIEQLAKKGE----NFIDLPYAVKGMDVSFSGILSYIETTAEEK-----------LK 244

Query: 314 QDRSVRADIAASFQRVAVRHLEERCERAIEWALKIEPSIKHLVVSGGVASNKYVRAQLTE 373
            +    AD+  S Q      L E  ERA+    K     K +++ GGV  N+ ++  +  
Sbjct: 245 NNECTPADLCYSLQETVFAMLVEITERAMAHCDK-----KDVLIVGGVGCNERLQEMMRT 304

Query: 374 VVRKKGLQLVCPPPSLCTDNGVMVAWTGL 403
           +  ++  +L       C DNG M+A+TGL
Sbjct: 305 MCSERDGKLFATDDRYCIDNGAMIAYTGL 308

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g17370 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22145	4.1e-190	71.82	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Arabid...	[more]
B3PQA4	2.0e-72	43.96	tRNA N6-adenosine threonylcarbamoyltransferase OS=Rhizobium etli (strain CIAT 65...	[more]
Q0P4K0	2.6e-72	42.98	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Xenopu...	[more]
Q0BPC9	3.4e-72	43.56	tRNA N6-adenosine threonylcarbamoyltransferase OS=Granulibacter bethesdensis (st...	[more]
Q2VYV2	5.9e-72	42.90	tRNA N6-adenosine threonylcarbamoyltransferase OS=Magnetospirillum magneticum (s...	[more]

BLAST of Cp4.1LG03g17370 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527336.1	0.0	100.00	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbit...	[more]
XP_022924665.1	0.0	98.71	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbit...	[more]
XP_022980286.1	0.0	97.64	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Cucurbit...	[more]
KAG6582553.1	0.0	98.07	putative tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial, partial ...	[more]
XP_038877779.1	2.04e-312	93.35	probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial [Benincas...	[more]

BLAST of Cp4.1LG03g17370 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFN5	0.0	98.71	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurb...	[more]
A0A6J1IT72	0.0	97.64	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucurb...	[more]
A0A0A0L938	2.16e-307	92.93	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumi...	[more]
A0A1S3AWD3	9.34e-306	92.72	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Cucumi...	[more]
A0A6J1D0B8	1.47e-303	89.74	Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial OS=Momord...	[more]

BLAST of Cp4.1LG03g17370 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45270.1	2.9e-191	71.82	glycoprotease 1	[more]
AT4G22720.1	1.5e-22	27.05	Actin-like ATPase superfamily protein	[more]
AT4G22720.2	1.5e-22	27.05	Actin-like ATPase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017861	Kae1/TsaD family	PRINTS	PR00789	OSIALOPTASE	coord: 149..169 score: 50.31 coord: 77..90 score: 64.29 coord: 227..248 score: 59.49 coord: 170..189 score: 37.83 coord: 204..216 score: 52.17 coord: 355..364 score: 60.0
IPR017861	Kae1/TsaD family	TIGRFAM	TIGR00329	TIGR00329	coord: 77..398 e-value: 6.6E-93 score: 309.2
IPR000905	Gcp-like domain	PFAM	PF00814	TsaD	coord: 96..399 e-value: 7.6E-90 score: 301.3
IPR000905	Gcp-like domain	PANTHER	PTHR11735	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE	coord: 71..443
IPR022450	tRNA N6-adenosine threonylcarbamoyltransferase, TsaD	TIGRFAM	TIGR03723	TIGR03723	coord: 76..405 e-value: 2.2E-106 score: 353.5
IPR022450	tRNA N6-adenosine threonylcarbamoyltransferase, TsaD	PANTHER	PTHR11735:SF6	TRNA N6-ADENOSINE THREONYLCARBAMOYLTRANSFERASE, MITOCHONDRIAL-RELATED	coord: 71..443
IPR022450	tRNA N6-adenosine threonylcarbamoyltransferase, TsaD	HAMAP	MF_01445	TsaD	coord: 74..432 score: 61.312195
None	No IPR available	GENE3D	3.30.420.40		coord: 202..385 e-value: 9.5E-123 score: 411.1
None	No IPR available	GENE3D	3.30.420.40		coord: 76..406 e-value: 9.5E-123 score: 411.1
IPR043129	ATPase, nucleotide binding domain	SUPERFAMILY	53067	Actin-like ATPase domain	coord: 75..399

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g17370.1	Cp4.1LG03g17370.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009793	embryo development ending in seed dormancy
biological_process	GO:0002949	tRNA threonylcarbamoyladenosine modification
cellular_component	GO:0005743	mitochondrial inner membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061711	N(6)-L-threonylcarbamoyladenine synthase activity