Cp4.1LG03g16360 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g16360
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionacidic endochitinase-like
LocationCp4.1LG03: 13304882 .. 13305919 (-)
RNA-Seq ExpressionCp4.1LG03g16360
SyntenyCp4.1LG03g16360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCTTGTTCTTCCCTCAGATGGCATCCTTCCAATTCCACGCTCTTCTTCTATTTCTCCTTTGTTTCTCACTCATCGCCGTCTCCACCGCCACCGGCCTCCGTGGTCTGGAGGTCGGAGTTGATTTGGACCTAAACAGCCTTCTCGACGCCTGCAACTCTGCCAATTACCAGACCATCAACATAGGTTTCCTCGTTTCACTTGGCAACGCCCAAACTCCCGAGATCAATTTAACCGCCTACTGCAATCCAACCACCGGCGACGGCTGCACTAAATTCAGCCAACAAATCAAGTCTTGTCAAGCCTTGGGCATCAAAATTATGCTCTCCATCGGCGGCGGAGACGGTAAATACAATCTGAACAATTTCACCGAGGCCACGAACTTCTCCACCTACCTATGGGGCAATTTCCTCGGCGGCCAATCAAATTCCCGTCCATTGAACGACGCCGTATTCGACGGCGTCGACATTACCAACGATAGAAGCTCCTGGGACAATTGGAGCAAACTCGGCGAAGAGCTGGGGAAATTATACGAAAAACAGGGGAGGAAATTTTACTTGTCCGCCGCTCCGCAATGTTCGTCTCTGGATTCGAGTGCTCATTCGATTCCACAACCAGGGATTTTCGACTATATTTCAGTCCAATTCTACGGGGATAATCTGGTTTGCCAGTACCTCAATGACCGGCTGGATGGGTTCTGGAAAAGTTGGAACATGTGGAAGACATTTCGTGCAGGCAAGGTGTTTGTGAAAATGCTAGCGGCTCCAATGGCGGAAGGGGTGGGATATATACGCCCCGATGTGTTCAGAACTGAAGTCTTGCCGGAGCTTCAACAGAGTTCGAACTTTGGCGGCGTGATTTTGTCATCTGAAGATCTTAAAGAAGGGTATAGTTCAGAGATTAACCCAAAAGTGTGTGGATCAGTTGGGGCGGAGTCGCAATCGGCGAATGAAATGGCGGTGTTTCCGATGGGCAATGGCTATGGTGGTCAACGAGGGTTTAGAAGTTGCAGAGGATCTTGCGTGATTATCTAG

mRNA sequence

ATGGCATCCTTGTTCTTCCCTCAGATGGCATCCTTCCAATTCCACGCTCTTCTTCTATTTCTCCTTTGTTTCTCACTCATCGCCGTCTCCACCGCCACCGGCCTCCGTGGTCTGGAGGTCGGAGTTGATTTGGACCTAAACAGCCTTCTCGACGCCTGCAACTCTGCCAATTACCAGACCATCAACATAGGTTTCCTCGTTTCACTTGGCAACGCCCAAACTCCCGAGATCAATTTAACCGCCTACTGCAATCCAACCACCGGCGACGGCTGCACTAAATTCAGCCAACAAATCAAGTCTTGTCAAGCCTTGGGCATCAAAATTATGCTCTCCATCGGCGGCGGAGACGGTAAATACAATCTGAACAATTTCACCGAGGCCACGAACTTCTCCACCTACCTATGGGGCAATTTCCTCGGCGGCCAATCAAATTCCCGTCCATTGAACGACGCCGTATTCGACGGCGTCGACATTACCAACGATAGAAGCTCCTGGGACAATTGGAGCAAACTCGGCGAAGAGCTGGGGAAATTATACGAAAAACAGGGGAGGAAATTTTACTTGTCCGCCGCTCCGCAATGTTCGTCTCTGGATTCGAGTGCTCATTCGATTCCACAACCAGGGATTTTCGACTATATTTCAGTCCAATTCTACGGGGATAATCTGGTTTGCCAGTACCTCAATGACCGGCTGGATGGGTTCTGGAAAAGTTGGAACATGTGGAAGACATTTCGTGCAGGCAAGGTGTTTGTGAAAATGCTAGCGGCTCCAATGGCGGAAGGGGTGGGATATATACGCCCCGATGTGTTCAGAACTGAAGTCTTGCCGGAGCTTCAACAGAGTTCGAACTTTGGCGGCGTGATTTTGTCATCTGAAGATCTTAAAGAAGGGTATAGTTCAGAGATTAACCCAAAAGTGTGTGGATCAGTTGGGGCGGAGTCGCAATCGGCGAATGAAATGGCGGTGTTTCCGATGGGCAATGGCTATGGTGGTCAACGAGGGTTTAGAAGTTGCAGAGGATCTTGCGTGATTATCTAG

Coding sequence (CDS)

ATGGCATCCTTGTTCTTCCCTCAGATGGCATCCTTCCAATTCCACGCTCTTCTTCTATTTCTCCTTTGTTTCTCACTCATCGCCGTCTCCACCGCCACCGGCCTCCGTGGTCTGGAGGTCGGAGTTGATTTGGACCTAAACAGCCTTCTCGACGCCTGCAACTCTGCCAATTACCAGACCATCAACATAGGTTTCCTCGTTTCACTTGGCAACGCCCAAACTCCCGAGATCAATTTAACCGCCTACTGCAATCCAACCACCGGCGACGGCTGCACTAAATTCAGCCAACAAATCAAGTCTTGTCAAGCCTTGGGCATCAAAATTATGCTCTCCATCGGCGGCGGAGACGGTAAATACAATCTGAACAATTTCACCGAGGCCACGAACTTCTCCACCTACCTATGGGGCAATTTCCTCGGCGGCCAATCAAATTCCCGTCCATTGAACGACGCCGTATTCGACGGCGTCGACATTACCAACGATAGAAGCTCCTGGGACAATTGGAGCAAACTCGGCGAAGAGCTGGGGAAATTATACGAAAAACAGGGGAGGAAATTTTACTTGTCCGCCGCTCCGCAATGTTCGTCTCTGGATTCGAGTGCTCATTCGATTCCACAACCAGGGATTTTCGACTATATTTCAGTCCAATTCTACGGGGATAATCTGGTTTGCCAGTACCTCAATGACCGGCTGGATGGGTTCTGGAAAAGTTGGAACATGTGGAAGACATTTCGTGCAGGCAAGGTGTTTGTGAAAATGCTAGCGGCTCCAATGGCGGAAGGGGTGGGATATATACGCCCCGATGTGTTCAGAACTGAAGTCTTGCCGGAGCTTCAACAGAGTTCGAACTTTGGCGGCGTGATTTTGTCATCTGAAGATCTTAAAGAAGGGTATAGTTCAGAGATTAACCCAAAAGTGTGTGGATCAGTTGGGGCGGAGTCGCAATCGGCGAATGAAATGGCGGTGTTTCCGATGGGCAATGGCTATGGTGGTCAACGAGGGTTTAGAAGTTGCAGAGGATCTTGCGTGATTATCTAG

Protein sequence

MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSSEINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII
Homology
BLAST of Cp4.1LG03g16360 vs. ExPASy Swiss-Prot
Match: P23472 (Hevamine-A OS=Hevea brasiliensis OX=3981 PE=1 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 2.2e-63
Identity = 135/292 (46.23%), Postives = 178/292 (60.96%), Query Frame = 0

Query: 16  ALLLFLLCFSLIAVSTATGLRGLEV--GVDLDLNSLLDACNSANYQTINIGFLVSLGNAQ 75
           A+LL LL  SLI  S+     G+ +  G + +  +L   C++  Y  +NI FL   GN Q
Sbjct: 7   AILLLLLAISLIMSSSHVDGGGIAIYWGQNGNEGTLTQTCSTRKYSYVNIAFLNKFGNGQ 66

Query: 76  TPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTY 135
           TP+INL  +CNP  G GCT  S  I+SCQ  GIK+MLS+GGG G Y L +  +A N + Y
Sbjct: 67  TPQINLAGHCNPAAG-GCTIVSNGIRSCQIQGIKVMLSLGGGIGSYTLASQADAKNVADY 126

Query: 136 LWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYLSAAPQ 195
           LW NFLGG+S+SRPL DAV DG+D   +  S   W  L   L   Y KQG+K YL+AAPQ
Sbjct: 127 LWNNFLGGKSSSRPLGDAVLDGIDFDIEHGSTLYWDDLARYL-SAYSKQGKKVYLTAAPQ 186

Query: 196 CSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKT-FRAGKVFVK 255
           C   D    +    G+FDY+ VQFY +N  CQY +  ++    SWN W T   AGK+F+ 
Sbjct: 187 CPFPDRYLGTALNTGLFDYVWVQFY-NNPPCQYSSGNINNIINSWNRWTTSINAGKIFLG 246

Query: 256 MLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSE--DLKEGYSSEI 303
           + AAP A G GY+ PDV  + +LPE+++S  +GGV+L S+  D K GYSS I
Sbjct: 247 LPAAPEAAGSGYVPPDVLISRILPEIKKSPKYGGVMLWSKFYDDKNGYSSSI 295

BLAST of Cp4.1LG03g16360 vs. ExPASy Swiss-Prot
Match: P36908 (Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.7e-58
Identity = 129/296 (43.58%), Postives = 183/296 (61.82%), Query Frame = 0

Query: 14  FHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVSLGNAQ 73
           F+ +   LL   LI  S A G+  +  G + +  SL DACN+ NYQ +NI FL + GN Q
Sbjct: 5   FNIIPSLLLISLLIKSSNAAGI-AVYWGQNGNEGSLQDACNTNNYQFVNIAFLSTFGNGQ 64

Query: 74  TPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTY 133
            P+INL  +C+P+T +GCTKFS +I++CQA GIK++LS+GGG G Y+LN+  EAT  + Y
Sbjct: 65  NPQINLAGHCDPST-NGCTKFSPEIQACQAKGIKVLLSLGGGAGSYSLNSAEEATTLANY 124

Query: 134 LWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYLSAAPQ 193
           LW NFLGG S SRPL DAV DG+D  +  S   ++ +L + L    +   +K YLSAAPQ
Sbjct: 125 LWNNFLGGTSTSRPLGDAVLDGIDF-DIESGGQHYDELAKALNGFSQ---QKVYLSAAPQ 184

Query: 194 CSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGKVFVKM 253
           C   D+   S  Q G+FDY+ VQFY +N  CQY N  ++    +WN W + +A +VF+ +
Sbjct: 185 CPYPDAHLDSAIQTGLFDYVWVQFY-NNPQCQYSNGNINNLVNAWNQWTSSQAKQVFLGV 244

Query: 254 LAAPMAEGVGYIRP-DVFRTEVLPELQQSSNFGGVIL--SSEDLKEGYSSEINPKV 307
            A+  A   G + P DV  ++VLP ++ S  +GGV++     D + GYS+ I   V
Sbjct: 245 PASDAAAPSGGLIPADVLTSQVLPAIKTSPKYGGVMIWDRFNDAQSGYSNAIKGSV 293

BLAST of Cp4.1LG03g16360 vs. ExPASy Swiss-Prot
Match: P29060 (Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.7e-58
Identity = 128/298 (42.95%), Postives = 169/298 (56.71%), Query Frame = 0

Query: 11  SFQFHALLLFLLCFSLIA--VSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVS 70
           SF   AL+LFL    L A  +    G  G E        SL D C + NY  +NI FLV 
Sbjct: 5   SFLLTALVLFLRALKLEAGDIVIYWGQNGNE-------GSLADTCATNNYAIVNIAFLVV 64

Query: 71  LGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEAT 130
            GN Q P +NL  +C+P  G  CT  S  I++CQ  GIK+MLS+GGG G Y L++  +A 
Sbjct: 65  FGNGQNPVLNLAGHCDPNAG-ACTGLSNDIRACQNQGIKVMLSLGGGAGSYFLSSADDAR 124

Query: 131 NFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYL 190
           N + YLW N+LGGQSN+RPL DAV DG+D   +  +  +W +L + L +    Q RK YL
Sbjct: 125 NVANYLWNNYLGGQSNTRPLGDAVLDGIDFDIEGGTTQHWDELAKTLSQF--SQQRKVYL 184

Query: 191 SAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGK 250
           +AAPQC   D+  +     G+FDY+ VQFY +N  CQY     D     WN W   +AGK
Sbjct: 185 TAAPQCPFPDTWLNGALSTGLFDYVWVQFY-NNPPCQYSGGSADNLKNYWNQWNAIQAGK 244

Query: 251 VFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSSEINPKV 307
           +F+ + AA  A G G+I  DV  ++VLP +  S  +GGV+L S+    GYSS I   V
Sbjct: 245 IFLGLPAAQGAAGSGFIPSDVLVSQVLPLINGSPKYGGVMLWSKFYDNGYSSAIKANV 291

BLAST of Cp4.1LG03g16360 vs. ExPASy Swiss-Prot
Match: P19172 (Acidic endochitinase OS=Arabidopsis thaliana OX=3702 GN=CHIB1 PE=2 SV=2)

HSP 1 Score: 222.2 bits (565), Expect = 8.8e-57
Identity = 124/288 (43.06%), Postives = 168/288 (58.33%), Query Frame = 0

Query: 19  LFLLCFSLIAVSTAT-GLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVSLGNAQTPEI 78
           LF +  SL   S A+ G   +  G + +  +L   C +  Y  +N+ FLV  GN QTPE+
Sbjct: 14  LFFISCSLSKPSDASRGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPEL 73

Query: 79  NLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTYLWGN 138
           NL  +CNP   + CT F  Q+K CQ+ GIK+MLS+GGG G Y++ +  +A   + YLW N
Sbjct: 74  NLAGHCNP-AANTCTHFGSQVKDCQSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNN 133

Query: 139 FLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYLSAAPQCSSL 198
           FLGG+S+SRPL DAV DG+D   +  S  +W  L   L K +  +GRK YL+ APQC   
Sbjct: 134 FLGGKSSSRPLGDAVLDGIDFNIELGSPQHWDDLARTLSK-FSHRGRKIYLTGAPQCPFP 193

Query: 199 DSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKT-FRAGKVFVKMLAA 258
           D    S      FDY+ +QFY +N  C Y +      + SWN W T   A K F+ + AA
Sbjct: 194 DRLMGSALNTKRFDYVWIQFY-NNPPCSYSSGNTQNLFDSWNKWTTSIAAQKFFLGLPAA 253

Query: 259 PMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSE--DLKEGYSSEI 303
           P A G GYI PDV  +++LP L++S  +GGV+L S+  D K GYSS I
Sbjct: 254 PEAAGSGYIPPDVLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSI 298

BLAST of Cp4.1LG03g16360 vs. ExPASy Swiss-Prot
Match: P51614 (Acidic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT3 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.5e-56
Identity = 123/295 (41.69%), Postives = 177/295 (60.00%), Query Frame = 0

Query: 17  LLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVSLGNAQTPE 76
           LL+ L   +L+  S A G+  +  G + +  +L   CN+  Y  +NI FL   GN QTPE
Sbjct: 10  LLISLSVLALLQTSYAGGI-AIYWGQNGNEGTLTQTCNTGKYSYVNIAFLNKFGNGQTPE 69

Query: 77  INLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTYLWG 136
           INL  +CNP + +GCT  S  I++CQ  GIK+MLSIGGG G Y+L++  +A N + YLW 
Sbjct: 70  INLAGHCNPAS-NGCTSVSTGIRNCQNRGIKVMLSIGGGAGSYSLSSSNDAQNVANYLWN 129

Query: 137 NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKL--YEKQGRKFYLSAAPQC 196
           NFLGGQS+SRPL DAV DG+D   +  S  +W  L   L ++   +++GRK YL+AAPQC
Sbjct: 130 NFLGGQSSSRPLGDAVLDGIDFDIELGSTLHWDDLARALSRIEFQQERGRKVYLTAAPQC 189

Query: 197 SSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMW-KTFRAGKVFVKM 256
              D    +    G+FDY+ VQFY +N  CQY +   +    SWN W  +  +   F+ +
Sbjct: 190 PFPDKVPGTALNTGLFDYVWVQFY-NNPPCQYSSGNTNNLLNSWNRWTSSINSTGSFMGL 249

Query: 257 LAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSE--DLKEGYSSEINPKV 307
            A+  A G G+I  +V  +++LP +++S  +GGV+L S+  D + GYSS I   V
Sbjct: 250 PASSAAAGRGFIPANVLTSQILPVIKRSPKYGGVMLWSKYYDDQSGYSSSIKSSV 301

BLAST of Cp4.1LG03g16360 vs. NCBI nr
Match: XP_023528415.1 (hevamine-A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699 bits (1803), Expect = 8.50e-254
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT
Sbjct: 7   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN
Sbjct: 67  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE
Sbjct: 127 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 187 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS
Sbjct: 247 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 345
           EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII
Sbjct: 307 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 351

BLAST of Cp4.1LG03g16360 vs. NCBI nr
Match: XP_022924633.1 (hevamine-A-like [Cucurbita moschata])

HSP 1 Score: 675 bits (1742), Expect = 1.69e-244
Identity = 335/345 (97.10%), Postives = 337/345 (97.68%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNS NYQT
Sbjct: 7   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSGNYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGG GKY+
Sbjct: 67  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGAGKYD 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEEL KLYE
Sbjct: 127 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELRKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 187 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAP AEG+GYIRPDVFRTEVLPELQQSS FGGVILSSEDLKEGYSS
Sbjct: 247 WKTFRAGKVFVKMLAAPKAEGLGYIRPDVFRTEVLPELQQSSKFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 345
           EINPKVCGSVGAESQSANEMAVFPMGN YGGQRGFRSCRGSCVII
Sbjct: 307 EINPKVCGSVGAESQSANEMAVFPMGNSYGGQRGFRSCRGSCVII 351

BLAST of Cp4.1LG03g16360 vs. NCBI nr
Match: XP_022979331.1 (hevamine-A-like [Cucurbita maxima])

HSP 1 Score: 671 bits (1732), Expect = 5.66e-243
Identity = 333/345 (96.52%), Postives = 336/345 (97.39%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRG EVGVDLDLNSLLDACNSANYQT
Sbjct: 7   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGPEVGVDLDLNSLLDACNSANYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGF+VSLG+AQTPEINLTAYCNPTTG GCTKFSQQIKSCQALGIKIMLSIGGG GKYN
Sbjct: 67  INIGFIVSLGSAQTPEINLTAYCNPTTGAGCTKFSQQIKSCQALGIKIMLSIGGGAGKYN 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE
Sbjct: 127 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQG+KFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 187 KQGKKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSS FGGVILSSEDLKEGYSS
Sbjct: 247 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSKFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 345
           EINPKVCGSVGAESQS NEMAVFPMGN YGGQRGF SCRGSCVII
Sbjct: 307 EINPKVCGSVGAESQSTNEMAVFPMGNSYGGQRGFGSCRGSCVII 351

BLAST of Cp4.1LG03g16360 vs. NCBI nr
Match: KAG6582640.1 (hypothetical protein SDJN03_22642, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 648 bits (1672), Expect = 2.05e-228
Identity = 321/329 (97.57%), Postives = 322/329 (97.87%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNS NYQT
Sbjct: 1   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSGNYQT 60

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQA GIKIMLSIGGGDGKYN
Sbjct: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQASGIKIMLSIGGGDGKYN 120

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE
Sbjct: 121 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQGRKFYLSAAPQCSSLDSS HSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 181 KQGRKFYLSAAPQCSSLDSSGHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAP AEG+GYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS
Sbjct: 241 WKTFRAGKVFVKMLAAPKAEGLGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGY 329
           EINPKVCGSVGAESQSANEMAVFPMGN Y
Sbjct: 301 EINPKVCGSVGAESQSANEMAVFPMGNSY 329

BLAST of Cp4.1LG03g16360 vs. NCBI nr
Match: KAG7019036.1 (hypothetical protein SDJN02_20913, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 645 bits (1665), Expect = 1.52e-227
Identity = 324/343 (94.46%), Postives = 326/343 (95.04%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNS NYQT
Sbjct: 7   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSGNYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQA GIKIMLSIGGGDGKYN
Sbjct: 67  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQASGIKIMLSIGGGDGKYN 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE
Sbjct: 127 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQGRKFYLSAAPQCSSLDSS HSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKS   
Sbjct: 187 KQGRKFYLSAAPQCSSLDSSGHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKS--- 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
                  KVFVKMLAAP AEG+GYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS
Sbjct: 247 -------KVFVKMLAAPKAEGLGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCV 343
           EINPKVCGSVGAESQSANEMAVFPMGN YGGQRGFRSCRGSC+
Sbjct: 307 EINPKVCGSVGAESQSANEMAVFPMGNSYGGQRGFRSCRGSCI 339

BLAST of Cp4.1LG03g16360 vs. ExPASy TrEMBL
Match: A0A6J1ED16 (hevamine-A-like OS=Cucurbita moschata OX=3662 GN=LOC111432066 PE=4 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 8.19e-245
Identity = 335/345 (97.10%), Postives = 337/345 (97.68%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNS NYQT
Sbjct: 7   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSGNYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGG GKY+
Sbjct: 67  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGAGKYD 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEEL KLYE
Sbjct: 127 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELRKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 187 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAP AEG+GYIRPDVFRTEVLPELQQSS FGGVILSSEDLKEGYSS
Sbjct: 247 WKTFRAGKVFVKMLAAPKAEGLGYIRPDVFRTEVLPELQQSSKFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 345
           EINPKVCGSVGAESQSANEMAVFPMGN YGGQRGFRSCRGSCVII
Sbjct: 307 EINPKVCGSVGAESQSANEMAVFPMGNSYGGQRGFRSCRGSCVII 351

BLAST of Cp4.1LG03g16360 vs. ExPASy TrEMBL
Match: A0A6J1IQH3 (hevamine-A-like OS=Cucurbita maxima OX=3661 GN=LOC111479086 PE=4 SV=1)

HSP 1 Score: 671 bits (1732), Expect = 2.74e-243
Identity = 333/345 (96.52%), Postives = 336/345 (97.39%), Query Frame = 0

Query: 1   MASLFFPQMASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQT 60
           MASLFFPQMASFQFHALLL LLCFSLIAVSTATGLRG EVGVDLDLNSLLDACNSANYQT
Sbjct: 7   MASLFFPQMASFQFHALLLSLLCFSLIAVSTATGLRGPEVGVDLDLNSLLDACNSANYQT 66

Query: 61  INIGFLVSLGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYN 120
           INIGF+VSLG+AQTPEINLTAYCNPTTG GCTKFSQQIKSCQALGIKIMLSIGGG GKYN
Sbjct: 67  INIGFIVSLGSAQTPEINLTAYCNPTTGAGCTKFSQQIKSCQALGIKIMLSIGGGAGKYN 126

Query: 121 LNNFTEATNFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 180
           LNNFTEATNFSTYLW NFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE
Sbjct: 127 LNNFTEATNFSTYLWDNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYE 186

Query: 181 KQGRKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 240
           KQG+KFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM
Sbjct: 187 KQGKKFYLSAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNM 246

Query: 241 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSS 300
           WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSS FGGVILSSEDLKEGYSS
Sbjct: 247 WKTFRAGKVFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSKFGGVILSSEDLKEGYSS 306

Query: 301 EINPKVCGSVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVII 345
           EINPKVCGSVGAESQS NEMAVFPMGN YGGQRGF SCRGSCVII
Sbjct: 307 EINPKVCGSVGAESQSTNEMAVFPMGNSYGGQRGFGSCRGSCVII 351

BLAST of Cp4.1LG03g16360 vs. ExPASy TrEMBL
Match: A0A0A0L410 (Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G120470 PE=4 SV=1)

HSP 1 Score: 541 bits (1395), Expect = 3.46e-192
Identity = 271/336 (80.65%), Postives = 287/336 (85.42%), Query Frame = 0

Query: 9   MASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVS 68
           MASF+F   LL  LCFSLIA STA   R  E+GV    N L+DACNS NYQ INI F VS
Sbjct: 1   MASFKFSIHLLTFLCFSLIAGSTANPRRSFELGVYSIKNGLIDACNSGNYQIINIVFTVS 60

Query: 69  LGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEAT 128
           LGNAQTPEIN+  YC  T  DGCTKFSQ+IKSCQALGIKIMLSIGGG GKYNLNNFTEAT
Sbjct: 61  LGNAQTPEINVIDYCTSTGVDGCTKFSQEIKSCQALGIKIMLSIGGGVGKYNLNNFTEAT 120

Query: 129 NFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYL 188
           NFSTYLW NFLGGQSNSRPLND VFDGVDITN+RSSWDNWSKLGEEL KLYEKQ +KFYL
Sbjct: 121 NFSTYLWNNFLGGQSNSRPLNDVVFDGVDITNERSSWDNWSKLGEELRKLYEKQRKKFYL 180

Query: 189 SAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGK 248
           SAAPQCSSLDSS+HSIPQPGIFDYISVQF+G+NLVCQYLN RL+GFWK WN WK F A K
Sbjct: 181 SAAPQCSSLDSSSHSIPQPGIFDYISVQFFGNNLVCQYLNGRLEGFWKFWNSWKMFNADK 240

Query: 249 VFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSSEINPKVCG 308
           VFVK+LA PMAE +GYIRPDVFRTEVLPELQQSS FGGVIL SEDL  GYSSEINPK CG
Sbjct: 241 VFVKLLAGPMAEDMGYIRPDVFRTEVLPELQQSSKFGGVILWSEDLDRGYSSEINPKACG 300

Query: 309 SVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVI 344
           SV A S+SANE+A FPMGN Y GQRGFRSCRGSCV+
Sbjct: 301 SVEAASESANEVAEFPMGNNYAGQRGFRSCRGSCVL 336

BLAST of Cp4.1LG03g16360 vs. ExPASy TrEMBL
Match: A0A1S3AVC5 (acidic endochitinase-like OS=Cucumis melo OX=3656 GN=LOC103483230 PE=4 SV=1)

HSP 1 Score: 540 bits (1392), Expect = 9.91e-192
Identity = 266/336 (79.17%), Postives = 290/336 (86.31%), Query Frame = 0

Query: 9   MASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVS 68
           M SFQF   LL  L FSLIAVST+   R  EVGV    N+L+D CNS NYQ IN+ F VS
Sbjct: 1   MPSFQFPIHLLTFLYFSLIAVSTSASRRSFEVGVYSTKNALIDVCNSGNYQIINVVFTVS 60

Query: 69  LGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEAT 128
           LGNAQTPEINL  +CN T  DGCTKFSQ+IK+CQA GIKIMLS+GGG GKYNLNNFTEAT
Sbjct: 61  LGNAQTPEINLIDHCNSTAVDGCTKFSQEIKACQASGIKIMLSVGGGSGKYNLNNFTEAT 120

Query: 129 NFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYL 188
           NFSTYLW NFLGGQSNSRPLND VFDGVDITN+RSSWDNWSKLGEELGKLYEKQG+KFYL
Sbjct: 121 NFSTYLWNNFLGGQSNSRPLNDVVFDGVDITNERSSWDNWSKLGEELGKLYEKQGKKFYL 180

Query: 189 SAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGK 248
           SAAPQCSSLDSS+HSIPQPGIFDYISVQF+GDNLVCQYLN R +GFWK WN+WKTF A K
Sbjct: 181 SAAPQCSSLDSSSHSIPQPGIFDYISVQFFGDNLVCQYLNGRFEGFWKFWNVWKTFSADK 240

Query: 249 VFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSSEINPKVCG 308
           VFV+MLA PMAE +GYI PDVFR+EVLPELQ+SS FGGVIL SED+ +GYSS+INPKVCG
Sbjct: 241 VFVQMLAGPMAEDMGYIPPDVFRSEVLPELQRSSKFGGVILWSEDIDKGYSSKINPKVCG 300

Query: 309 SVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVI 344
           SV A S+SANE+A FPMGN Y GQRGFRSCRGSCV+
Sbjct: 301 SVEAASESANEIAEFPMGNNYVGQRGFRSCRGSCVL 336

BLAST of Cp4.1LG03g16360 vs. ExPASy TrEMBL
Match: A0A5A7U0K3 (Acidic endochitinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001500 PE=4 SV=1)

HSP 1 Score: 539 bits (1388), Expect = 4.03e-191
Identity = 266/336 (79.17%), Postives = 289/336 (86.01%), Query Frame = 0

Query: 9   MASFQFHALLLFLLCFSLIAVSTATGLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVS 68
           M SFQF   LL  L FSLIAVSTA   R  EVGV    N+L+  CNS NYQ IN+ F VS
Sbjct: 1   MPSFQFPIHLLTFLYFSLIAVSTAASRRSFEVGVYSTRNALIGVCNSGNYQIINVVFTVS 60

Query: 69  LGNAQTPEINLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEAT 128
           LGNAQTPEINL  +CN T  DGCTKFSQ+IK+CQA GIKIMLS+GGG GKYNLNNFTEAT
Sbjct: 61  LGNAQTPEINLIDHCNSTAVDGCTKFSQEIKACQASGIKIMLSVGGGSGKYNLNNFTEAT 120

Query: 129 NFSTYLWGNFLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYL 188
           NFSTYLW NFLGGQSNSRPLND VFDGVDITN+RSSWDNWSKLGEELGKLYEKQG+KFYL
Sbjct: 121 NFSTYLWNNFLGGQSNSRPLNDVVFDGVDITNERSSWDNWSKLGEELGKLYEKQGKKFYL 180

Query: 189 SAAPQCSSLDSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKTFRAGK 248
           SAAPQCSSLDSS+HSIPQPGIFDYISVQF+GDNLVCQYLN R +GFWK WN+WKTF A K
Sbjct: 181 SAAPQCSSLDSSSHSIPQPGIFDYISVQFFGDNLVCQYLNGRFEGFWKFWNVWKTFSADK 240

Query: 249 VFVKMLAAPMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSEDLKEGYSSEINPKVCG 308
           VFV+MLA PMAE +GYI PDVFR+EVLPELQ+SS FGGVIL SED+ +GYSS+INPKVCG
Sbjct: 241 VFVQMLAGPMAEDMGYIPPDVFRSEVLPELQRSSKFGGVILWSEDIDKGYSSKINPKVCG 300

Query: 309 SVGAESQSANEMAVFPMGNGYGGQRGFRSCRGSCVI 344
           SV A S+SANE+A FPMGN Y GQRGFRSCRGSCV+
Sbjct: 301 SVEAASESANEIAEFPMGNNYVGQRGFRSCRGSCVL 336

BLAST of Cp4.1LG03g16360 vs. TAIR 10
Match: AT5G24090.1 (chitinase A )

HSP 1 Score: 222.2 bits (565), Expect = 6.3e-58
Identity = 124/288 (43.06%), Postives = 168/288 (58.33%), Query Frame = 0

Query: 19  LFLLCFSLIAVSTAT-GLRGLEVGVDLDLNSLLDACNSANYQTINIGFLVSLGNAQTPEI 78
           LF +  SL   S A+ G   +  G + +  +L   C +  Y  +N+ FLV  GN QTPE+
Sbjct: 14  LFFISCSLSKPSDASRGGIAIYWGQNGNEGNLSATCATGRYAYVNVAFLVKFGNGQTPEL 73

Query: 79  NLTAYCNPTTGDGCTKFSQQIKSCQALGIKIMLSIGGGDGKYNLNNFTEATNFSTYLWGN 138
           NL  +CNP   + CT F  Q+K CQ+ GIK+MLS+GGG G Y++ +  +A   + YLW N
Sbjct: 74  NLAGHCNP-AANTCTHFGSQVKDCQSRGIKVMLSLGGGIGNYSIGSREDAKVIADYLWNN 133

Query: 139 FLGGQSNSRPLNDAVFDGVDITNDRSSWDNWSKLGEELGKLYEKQGRKFYLSAAPQCSSL 198
           FLGG+S+SRPL DAV DG+D   +  S  +W  L   L K +  +GRK YL+ APQC   
Sbjct: 134 FLGGKSSSRPLGDAVLDGIDFNIELGSPQHWDDLARTLSK-FSHRGRKIYLTGAPQCPFP 193

Query: 199 DSSAHSIPQPGIFDYISVQFYGDNLVCQYLNDRLDGFWKSWNMWKT-FRAGKVFVKMLAA 258
           D    S      FDY+ +QFY +N  C Y +      + SWN W T   A K F+ + AA
Sbjct: 194 DRLMGSALNTKRFDYVWIQFY-NNPPCSYSSGNTQNLFDSWNKWTTSIAAQKFFLGLPAA 253

Query: 259 PMAEGVGYIRPDVFRTEVLPELQQSSNFGGVILSSE--DLKEGYSSEI 303
           P A G GYI PDV  +++LP L++S  +GGV+L S+  D K GYSS I
Sbjct: 254 PEAAGSGYIPPDVLTSQILPTLKKSRKYGGVMLWSKFWDDKNGYSSSI 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P234722.2e-6346.23Hevamine-A OS=Hevea brasiliensis OX=3981 PE=1 SV=2[more]
P369082.7e-5843.58Acidic endochitinase OS=Cicer arietinum OX=3827 PE=2 SV=1[more]
P290602.7e-5842.95Acidic endochitinase OS=Nicotiana tabacum OX=4097 PE=1 SV=1[more]
P191728.8e-5743.06Acidic endochitinase OS=Arabidopsis thaliana OX=3702 GN=CHIB1 PE=2 SV=2[more]
P516141.5e-5641.69Acidic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023528415.18.50e-254100.00hevamine-A-like [Cucurbita pepo subsp. pepo][more]
XP_022924633.11.69e-24497.10hevamine-A-like [Cucurbita moschata][more]
XP_022979331.15.66e-24396.52hevamine-A-like [Cucurbita maxima][more]
KAG6582640.12.05e-22897.57hypothetical protein SDJN03_22642, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7019036.11.52e-22794.46hypothetical protein SDJN02_20913, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A6J1ED168.19e-24597.10hevamine-A-like OS=Cucurbita moschata OX=3662 GN=LOC111432066 PE=4 SV=1[more]
A0A6J1IQH32.74e-24396.52hevamine-A-like OS=Cucurbita maxima OX=3661 GN=LOC111479086 PE=4 SV=1[more]
A0A0A0L4103.46e-19280.65Glyco_hydro_18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G120... [more]
A0A1S3AVC59.91e-19279.17acidic endochitinase-like OS=Cucumis melo OX=3656 GN=LOC103483230 PE=4 SV=1[more]
A0A5A7U0K34.03e-19179.17Acidic endochitinase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
Match NameE-valueIdentityDescription
AT5G24090.16.3e-5843.06chitinase A [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 41..305
e-value: 1.1E-64
score: 220.4
NoneNo IPR availablePANTHERPTHR45708ENDOCHITINASEcoord: 20..304
NoneNo IPR availablePANTHERPTHR45708:SF22ACIDIC ENDOCHITINASEcoord: 20..304
IPR001223Glycoside hydrolase family 18, catalytic domainPFAMPF00704Glyco_hydro_18coord: 54..219
e-value: 2.9E-16
score: 60.1
IPR001223Glycoside hydrolase family 18, catalytic domainPROSITEPS51910GH18_2coord: 38..309
score: 21.060699
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 47..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g16360.1Cp4.1LG03g16360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0004568 chitinase activity