Cp4.1LG03g16000 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g16000
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cytochrome b5-like
Location	Cp4.1LG03: 13533592 .. 13535416 (+)
RNA-Seq Expression	Cp4.1LG03g16000
Synteny	Cp4.1LG03g16000

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTGAAGTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAACCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTGTAAGTAACCCCACATTCTTCCTCGGTTCTTCCGTGTTTGATTCCTCTGATTCTCCAAGTTAATCGACTGCTTCATTGTGGATTGAGCTTCATTTTTCTTTGCAGTTGATTTTTTCAGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTCTGATTCAACAATCTCTCTTCTAATCTTCATTTTGTCTAATTTTTGGTTTACAACTCGAATCGTATTTGCCAATGGAACAGTAGACTTAAATGAGATCCGATAGACGGATGGGAGATTGAGCCTATTTGTTTCTTCATCTTTCTCCTAACAATTTTTTAACTCATTACTCGAGAAACGACAGGAACTTGTTCTTCATCTTTTCTTCTGATGTGAAATCAGTTGTTGAGGATTAAAACATAAATCCTATCCATGTGTGAGACGTATTTCGTGGAATTCATTTAACAAGCACTTGTTCAAGAGGAAAAACTCTGGTATTGTAGTTTGCAATGAATCTAAGCTCCAACTTTCATCAAACAAGAATTTTAGGCCTCAAACTTCTTTTACGTTTAACAACATAGGAAGGAGATTTGATTTCCTCACTTAATCACATTTTCTCTCCCTTCAAACACCGATTTGGCCTCAAACATTCCTTTTCGGTCCATTTGCTCGAAATTTACAATTTAAACAACCATGCTCGTGAAATTCACTAATTCAACCACAAAACCCTGCAAAATCTTAGTCCTGCGAATAACAGATTATGTGTACTATCAGTTATCTCTGTATTCGTTCTTCTCCTAGATCTACCTAAAATAAGACACTTTCAATTTGGGTGATAAGATTTGTATTTTTTCTATCCTCTTCCTTTATATTGCTGTTGTGTTTCGTTGCCTTTCCCCAAAGTAGTTTTTGTGATCCATGTTAGGTGTATGATGTCACCTCCTTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGGTAAGTCCAACTTGATTACTTTTTTCTATTTGAAGATATCGAACAAAAATAATCACACCACGTCATGAATGATCGGAGAAACAATCTTGAATGTGTGCAGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGGTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCACCACCACCACCAACAACAACGTCATCAGATGAATCTCCAGGATCTTTGATAAAAGTATTGCAGGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAAGCGAAATTTGTCCAACCATAGATGCCTTGAGTTTTGCAATAAGCCAGAGCTGTGTAGTAGAAATTCTATCATGTTCTGTTGAAGACATTCTTTGTTATCTATCTAGGTTTCTCAGCCACAGCCTTCTTGCTAATATCTGAGAAGGGACTTTTCATTTGCCTCTTCTTCATTCCTTATCCTGTTTTCTGTCTTCTGTTCTACAATATTCAACTTTGTGTGCATGATTTAAC

mRNA sequence

AGTGAAGTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAACCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTTTGATTTTTTCAGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCTTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGGTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCACCACCACCACCAACAACAACGTCATCAGATGAATCTCCAGGATCTTTGATAAAAGTATTGCAGGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAAGCGAAATTTGTCCAACCATAGATGCCTTGAGTTTTGCAATAAGCCAGAGCTGTGTAGTAGAAATTCTATCATGTTCTGTTGAAGACATTCTTTGTTATCTATCTAGGTTTCTCAGCCACAGCCTTCTTGCTAATATCTGAGAAGGGACTTTTCATTTGCCTCTTCTTCATTCCTTATCCTGTTTTCTGTCTTCTGTTCTACAATATTCAACTTTGTGTGCATGATTTAAC

Coding sequence (CDS)

AGTGAAGTGAAATTGTTGCAAGAAAACAAATCCATTCGCGGGGTGTCATTTAGCCAGCGAAACCGGCCAGATAAAAGTCGCAAGAAATGGCTGGTAGTGGTAGTCAATGGAGAAGAAACCGTGCTGATTTGGGCTGTTTCTCCAATTTCCTTCAACGTTCATTCTTTGATTTTTTCAGAAATGGCTTCGGATCCTAAGGTTTTCGTCTTCGATGAAGTGGCTAAACACAATCACCAGCAGGATTGTTGGGTTATAATCTCTGGAAAGGTGTATGATGTCACCTCCTTTCTAGAAGATCATCCTGGAGGTGATGAAGTCTTGCTGTTAGCTACTGAAAGAGATGCCACTGATGATTTTGAAGAAGTGGGTCACAGTTTAACTGCGTCGGAGCAAATGGAAGAATATTATGTTGGTAATCTGGACATGGCTACCCTTCCAAAGGCAGGTGCCAATCGACCACCAGCACCCCAATCGGGGACAGCGACAACAGCAGCAGCAAAACCAACGGCAGCAGCACCACCACCACCAACAACAACGTCATCAGATGAATCTCCAGGATCTTTGATAAAAGTATTGCAGGTTCTGATTCCCCTGCTGATTTTGGGTATTGCATTTTATATGCAATACTATGGCAAGAAACAGTAA

Protein sequence

SEVKLLQENKSIRGVSFSQRNRPDKSRKKWLVVVVNGEETVLIWAVSPISFNVHSLIFSEMASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFEEVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTSSDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ

Homology

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BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.5e-35
Identity = 77/152 (50.66%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL+DHPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS TA   ++EYYVG++D AT+P      PP     T+T A A             +
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPP-----TSTKAVA-------------T 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGK 213
            D+S   +IK+LQ L+PLLILG+AF ++YY K
Sbjct: 121 QDKSSDFVIKLLQFLVPLLILGLAFGIRYYTK 134

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BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 3.8e-34
Identity = 76/154 (49.35%), Postives = 105/154 (68.18%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MAS+ KV  F+EV++HN  +DCW+IISGKVYDVT F++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MASEKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS TA + ME+YY+G +D +T+P                   A  T  AP  P   +
Sbjct: 61  DVGHSDTARDMMEKYYIGEIDSSTVP-------------------ATRTYVAPVQP-AYN 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 215
            D++P  +IK+LQ L+P+LILG+A  ++ Y KK+
Sbjct: 121 QDKTPEFMIKILQFLVPILILGLALVVRQYTKKE 134

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BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot
Match: Q42342 (Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 1.1e-33
Identity = 74/153 (48.37%), Postives = 105/153 (68.63%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT F++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS TA + M++Y++G +D +++P                   A  T  AP  P   +
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVP-------------------ATRTYVAPQQP-AYN 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKK 214
            D++P  +IK+LQ L+P+LILG+A  +++Y KK
Sbjct: 121 QDKTPEFIIKILQFLVPILILGLALVVRHYTKK 133

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BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.5e-33
Identity = 72/153 (47.06%), Postives = 101/153 (66.01%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M  + KVF   EV++HN+ +DCW++ISGKVYDVT FL+DHPGGDEVLL AT +DATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS +A   ++EYYVG++D AT+P                      T   PP     +
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIP--------------------TKTKYTPPNQPHYN 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKK 214
            D++   ++K+LQ L+PL+ILG+AF +++Y K+
Sbjct: 121 QDKTSEFVVKLLQFLVPLIILGVAFGIRFYTKQ 133

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BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 140.6 bits (353), Expect = 2.1e-32
Identity = 71/149 (47.65%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 66  KVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFEEVGHS 125
           KV+  +EVAKHN + DCW+II GKVY+V+ FLEDHPGGD+VLL +T +DATDDFE+VGHS
Sbjct: 7   KVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 66

Query: 126 LTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTSSDESP 185
            TA   M+EYYVG++D +T+P                      T   PP     + D++P
Sbjct: 67  TTARAMMDEYYVGDIDTSTIPAR--------------------TKYVPPKQPHYNQDKTP 126

Query: 186 GSLIKVLQVLIPLLILGIAFYMQYYGKKQ 215
             +IK+LQ L+PL ILG+A  ++ Y K +
Sbjct: 127 EFIIKILQFLVPLAILGLAVAIRIYTKSE 135

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BLAST of Cp4.1LG03g16000 vs. NCBI nr
Match: KAG6582691.1 (Cytochrome B5 isoform D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 376 bits (966), Expect = 1.47e-130
Identity = 200/219 (91.32%), Postives = 206/219 (94.06%), Query Frame = 0


Query: 1   SEVKLLQENKSIRGVSFSQRNRPDKSRKKWLVVVVNGEETVLIWAVSPISFNVHSLIFSE 60
           +EVKLLQENKSIRGVSFSQRNRPDKSRKKWLVVV+NGEETVLIWAVSPISFNVHSLI SE
Sbjct: 5   TEVKLLQENKSIRGVSFSQRNRPDKSRKKWLVVVINGEETVLIWAVSPISFNVHSLIVSE 64

Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHN+QQDCWVIISGKVYDVTS LEDHPGGDEVLLLATERDATDDFE
Sbjct: 65  MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 124

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPP---- 180
           EVGHSLTASEQMEEYYVGN+DM+TLPKA ANRPPAPQSGTATTAAAKPTAAA P      
Sbjct: 125 EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSGTATTAAAKPTAAAEPKAAAPT 184

Query: 181 -TTTSSDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
            TTTSS ESPGSL KVLQVLIPLLILGIA+YMQYYGKKQ
Sbjct: 185 TTTTSSAESPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 223

[Close]

BLAST of Cp4.1LG03g16000 vs. NCBI nr
Match: XP_023527392.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298 bits (763), Expect = 1.11e-100
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS
Sbjct: 61  EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ
Sbjct: 121 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 154

[Close]

BLAST of Cp4.1LG03g16000 vs. NCBI nr
Match: XP_022979314.1 (cytochrome b5-like [Cucurbita maxima])

HSP 1 Score: 275 bits (702), Expect = 1.97e-91
Identity = 146/154 (94.81%), Postives = 147/154 (95.45%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTS LE+HPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           EVGHSLTASEQMEEYYVGNLDMATLPKA ANRPPAPQSGTATTAAAKPTAAA   PTTTS
Sbjct: 61  EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAA---PTTTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           S ESPGSLIKV QVLIPLLILGIAFYMQYYGKKQ
Sbjct: 121 SAESPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 151

[Close]

BLAST of Cp4.1LG03g16000 vs. NCBI nr
Match: XP_022924404.1 (cytochrome b5-like [Cucurbita moschata] >KAG7019088.1 Cytochrome B5 isoform D, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 265 bits (677), Expect = 1.65e-87
Identity = 143/159 (89.94%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHN+QQDCWVIISGKVYDVTS LEDHPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPP---- 180
           EVGHSLTASEQMEEYYVGN+DM+TLPKA ANRPPAPQSGTATTAAAKPTAAA P      
Sbjct: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSGTATTAAAKPTAAAEPKAAAPT 120

Query: 181 -TTTSSDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
            TTTSS ESPGSL KVLQVLIPLLILGIA+YMQYYGKKQ
Sbjct: 121 TTTTSSAESPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 159

[Close]

BLAST of Cp4.1LG03g16000 vs. NCBI nr
Match: XP_004133794.1 (cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_010413 [Cucumis sativus])

HSP 1 Score: 214 bits (544), Expect = 1.76e-67
Identity = 110/154 (71.43%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT FLEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
            V HSL A+E+ME+YY+GN+DM+T+PK   +RPPA +S +A T AA            TS
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAAT---------EKTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           S +S GSLIKVLQVLIPLLI+G+AFY+QYYGKKQ
Sbjct: 121 SAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL
Match: A0A6J1IWB8 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111479076 PE=4 SV=1)

HSP 1 Score: 275 bits (702), Expect = 9.52e-92
Identity = 146/154 (94.81%), Postives = 147/154 (95.45%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTS LE+HPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSLLEEHPGGDEVLLLATERDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           EVGHSLTASEQMEEYYVGNLDMATLPKA ANRPPAPQSGTATTAAAKPTAAA   PTTTS
Sbjct: 61  EVGHSLTASEQMEEYYVGNLDMATLPKAVANRPPAPQSGTATTAAAKPTAAA---PTTTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           S ESPGSLIKV QVLIPLLILGIAFYMQYYGKKQ
Sbjct: 121 SAESPGSLIKVFQVLIPLLILGIAFYMQYYGKKQ 151

[Close]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL
Match: A0A6J1E925 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111431914 PE=4 SV=1)

HSP 1 Score: 265 bits (677), Expect = 8.00e-88
Identity = 143/159 (89.94%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPKVFVFDEVAKHN+QQDCWVIISGKVYDVTS LEDHPGGDEVLLLATERDATDDFE
Sbjct: 1   MASDPKVFVFDEVAKHNNQQDCWVIISGKVYDVTSLLEDHPGGDEVLLLATERDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPP---- 180
           EVGHSLTASEQMEEYYVGN+DM+TLPKA ANRPPAPQSGTATTAAAKPTAAA P      
Sbjct: 61  EVGHSLTASEQMEEYYVGNVDMSTLPKAVANRPPAPQSGTATTAAAKPTAAAEPKAAAPT 120

Query: 181 -TTTSSDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
            TTTSS ESPGSL KVLQVLIPLLILGIA+YMQYYGKKQ
Sbjct: 121 TTTTSSAESPGSLKKVLQVLIPLLILGIAYYMQYYGKKQ 159

[Close]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL
Match: A0A0A0L720 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 214 bits (544), Expect = 8.53e-68
Identity = 110/154 (71.43%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT FLEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
            V HSL A+E+ME+YY+GN+DM+T+PK   +RPPA +S +A T AA            TS
Sbjct: 61  TVSHSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAAT---------EKTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           S +S GSLIKVLQVLIPLLI+G+AFY+QYYGKKQ
Sbjct: 121 SAQSSGSLIKVLQVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL
Match: A0A1S3AV33 (cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1)

HSP 1 Score: 209 bits (532), Expect = 5.50e-66
Identity = 107/154 (69.48%), Postives = 128/154 (83.12%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT FLEDHPGGDEVLLLATE+DAT+DFE
Sbjct: 1   MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
            VGHSL+A+E+ME+YY+GN+DM+T+PK     P +  + T T AAA+          TTS
Sbjct: 61  TVGHSLSATEEMEKYYIGNIDMSTVPKVDYRPPASKSASTETKAAAE----------TTS 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           S++S GSLIKVLQ+LIPLLI+ +AFY+QYYGKKQ
Sbjct: 121 SNQSSGSLIKVLQLLIPLLIIVVAFYLQYYGKKQ 144

[Close]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL
Match: A0A5A7U0D0 (Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00740 PE=3 SV=1)

HSP 1 Score: 210 bits (534), Expect = 1.41e-64
Identity = 118/216 (54.63%), Postives = 154/216 (71.30%), Query Frame = 0


Query: 4   KLLQENKSIRGVSFSQRNR-----PDKSRKKWLVVVVNGEETVLIWAVSPISFNVHSLIF 63
           K L E ++ RG  FS++ +     P ++ ++ LV+ ++   T+              ++ 
Sbjct: 74  KKLPEVETDRGSCFSEKPKAAPEPPTRNLRRLLVLEIHHLATI----------EFPKIVL 133

Query: 64  SEMASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDD 123
             MASDPK+FVFDEVAKHNHQ DCW+IISGKVYDVT FLEDHPGGDEVLLLATE+DAT+D
Sbjct: 134 --MASDPKLFVFDEVAKHNHQGDCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATED 193

Query: 124 FEEVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTT 183
           FE VGHSL+A+E+ME+YY+GN+DM+T+PK     P +  + T T AAA+          T
Sbjct: 194 FETVGHSLSATEEMEKYYIGNIDMSTVPKVDYRPPASKSASTETKAAAE----------T 253

Query: 184 TSSDESPGSLIKVLQVLIPLLILGIAFYMQYYGKKQ 214
           TSS++S GSLIKVLQ+LIPLLI+ +AFY+QYYGKKQ
Sbjct: 254 TSSNQSSGSLIKVLQLLIPLLIIVVAFYLQYYGKKQ 267

[Close]

BLAST of Cp4.1LG03g16000 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 149.4 bits (376), Expect = 3.2e-36
Identity = 77/152 (50.66%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M  D KVF   EV++H+  +DCW++I GKVYDVT FL+DHPGGDEV+L +T +DATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS TA   ++EYYVG++D AT+P      PP     T+T A A             +
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPP-----TSTKAVA-------------T 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGK 213
            D+S   +IK+LQ L+PLLILG+AF ++YY K
Sbjct: 121 QDKSSDFVIKLLQFLVPLLILGLAFGIRYYTK 134

[Close]

BLAST of Cp4.1LG03g16000 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 144.8 bits (364), Expect = 7.9e-35
Identity = 74/153 (48.37%), Postives = 105/153 (68.63%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M+SD KV  F+EV+KHN  +DCW+IISGKVYDVT F++DHPGGDEVLL +T +DAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS TA + M++Y++G +D +++P                   A  T  AP  P   +
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVP-------------------ATRTYVAPQQP-AYN 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGKK 214
            D++P  +IK+LQ L+P+LILG+A  +++Y KK
Sbjct: 121 QDKTPEFIIKILQFLVPILILGLALVVRHYTKK 133

[Close]

BLAST of Cp4.1LG03g16000 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 135.6 bits (340), Expect = 4.8e-32
Identity = 69/152 (45.39%), Postives = 94/152 (61.84%), Query Frame = 0


Query: 61  MASDPKVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFE 120
           M  + K+F   EV++HN   DCW++I+GKVY+VT FLEDHPGGD+VLL +T +DATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 121 EVGHSLTASEQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTS 180
           +VGHS +A E ME+YYVG +D  T+P                    K     PP     +
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIP--------------------KKVKYTPPKQPHYN 120

Query: 181 SDESPGSLIKVLQVLIPLLILGIAFYMQYYGK 213
            D++   +IK+LQ L+PL ILG+A  ++ Y K
Sbjct: 121 QDKTSEFIIKLLQFLVPLAILGLAVGIRIYTK 132

[Close]

BLAST of Cp4.1LG03g16000 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 115.5 bits (288), Expect = 5.1e-26
Identity = 61/144 (42.36%), Postives = 89/144 (61.81%), Query Frame = 0


Query: 70  FDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFEEVGHSLTAS 129
           F +VAKH  + DCW++I GKVYD+++F+++HPGGD VLL  T +DA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 130 EQMEEYYVGNLDMATLPKAGANRPPAPQSGTATTAAAKPTAAAPPPPTTTSSDESPGSLI 189
           E M++Y +G++D +T+P      PP  +  TA                 T+ +ES     
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTA---------------AETTKEESGK--- 126

Query: 190 KVLQVLIPLLILGIAFYMQYYGKK 214
           K+L  LIPLLILG+AF +++Y  K
Sbjct: 127 KLLIYLIPLLILGVAFALRFYNNK 132

[Close]

BLAST of Cp4.1LG03g16000 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 112.1 bits (279), Expect = 5.7e-25
Identity = 46/82 (56.10%), Postives = 64/82 (78.05%), Query Frame = 0


Query: 66  KVFVFDEVAKHNHQQDCWVIISGKVYDVTSFLEDHPGGDEVLLLATERDATDDFEEVGHS 125
           K++  +E A HN Q DCWV+I GKVYDV+S++++HPGGD+VLL    +DATDDFE+ GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 126 LTASEQMEEYYVGNLDMATLPK 148
             A E ME+Y++G LD ++LP+
Sbjct: 66  KDARELMEKYFIGELDESSLPE 87

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g16000 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZWT2	4.5e-35	50.66	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
P40934	3.8e-34	49.35	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]
Q42342	1.1e-33	48.37	Cytochrome b5 isoform E OS=Arabidopsis thaliana OX=3702 GN=CYTB5-E PE=1 SV=2	[more]
P49098	2.5e-33	47.06	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	2.1e-32	47.65	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of Cp4.1LG03g16000 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6582691.1	1.47e-130	91.32	Cytochrome B5 isoform D, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023527392.1	1.11e-100	100.00	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022979314.1	1.97e-91	94.81	cytochrome b5-like [Cucurbita maxima]	[more]
XP_022924404.1	1.65e-87	89.94	cytochrome b5-like [Cucurbita moschata] >KAG7019088.1 Cytochrome B5 isoform D, p...	[more]
XP_004133794.1	1.76e-67	71.43	cytochrome b5 [Cucumis sativus] >KGN56392.1 hypothetical protein Csa_010413 [Cuc...	[more]

BLAST of Cp4.1LG03g16000 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IWB8	9.52e-92	94.81	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111479076 PE=4 SV=1	[more]
A0A6J1E925	8.00e-88	89.94	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111431914 PE=4 SV=1	[more]
A0A0A0L720	8.53e-68	71.43	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3AV33	5.50e-66	69.48	cytochrome b5-like OS=Cucumis melo OX=3656 GN=LOC103483149 PE=3 SV=1	[more]
A0A5A7U0D0	1.41e-64	54.63	Cytochrome b5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G0...	[more]

BLAST of Cp4.1LG03g16000 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48810.1	3.2e-36	50.66	cytochrome B5 isoform D	[more]
AT5G53560.1	7.9e-35	48.37	cytochrome B5 isoform E	[more]
AT2G32720.1	4.8e-32	45.39	cytochrome B5 isoform B	[more]
AT2G46650.1	5.1e-26	42.36	cytochrome B5 isoform C	[more]
AT1G26340.1	5.7e-25	56.10	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 90..100 score: 68.18 coord: 115..122 score: 44.13 coord: 128..140 score: 36.71 coord: 100..114 score: 46.57
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 68..141 e-value: 6.6E-27 score: 105.4
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 71..140 e-value: 3.5E-23 score: 81.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 65..141 score: 27.712755
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 59..144 e-value: 2.5E-31 score: 109.5
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 63..144
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 147..184
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 61..212
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 61..212
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 96..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g16000.1	Cp4.1LG03g16000.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding