Cp4.1LG03g14840 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g14840
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmitogen-activated protein kinase kinase 5-like
LocationCp4.1LG03: 8476797 .. 8477943 (-)
RNA-Seq ExpressionCp4.1LG03g14840
SyntenyCp4.1LG03g14840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGGATTGGATTCTTCTTCTTCTTCATCATCGTCATCAGCGCAGATGAGACCGCTCCAGCCGCCACAGGGTGTGAATCCGACCACCGATCGTCCTCGTCGCCGTCCCAATCTAACTCTTCCTCTTCCGCAGCGGGACAATACGTCTTTGGCGGTTCCTCTCCCTTTGCCTCCTACCTCCTCCAACGCTGCGCCTCCTTCCACCAGTCAACTTCACAACGGCAACCGCCCGCCGGATCCTCTTCCTCAGCAGCATCAGCCTTTTAGCCTTGCGGACTTCGAGAGAGTGAGCCGAATCGGAAGTGGATGTGGAGGTACCGTCTACAAAGTGATTCACCGACCTTCTGGCCGCGTCTATGCTCTCAAGGTTATTTATGGTAACCATGAGGATGCGGTTAGGCTCCAGATGGGCCGAGAGGTCAAGATCCTTCGCGACGTTGACAATCCCTATGTGGTCAAGTGCCATGATATGTTCGATCATAACGGGGAAATCCAGGTTCTCCTTGAGTACATGGATCGGGGCTCTTTGGAAGGAACGCATATCCCTCAGGAGCACCATCTCTCTGATTTGGCTCGCCAGATTCTTAGTGGCCTCGCCTATCTCCATAGCCGCCGCATCGTCCATCGCGACATCAAGCCTTCGAATCTCCTCATCAATTCCCGAAGGCAGGTTAAGATCGCTGATTTCGGCGTCGGTCGAATCTTGGAACAGACTATGGATCCTTGCAATTCCTCGGTTGGAACTATTGCGTACATGAGCCCTGAGAGAATCAATTCCGATCTAAATCAGGGCCAGTACAATGGCTATGCTGGGGACATTTGGAGCTTTGGGGTTAGCATATTGGAATTCTATCTCGGACGGTTTCCTCTCGCTGTCGAGAGGCCTGGGGATTGGGCGAGCTTGATGTGCGCTATATGTATGGCTCAGCCACCGGAGGCGCCGCCAACTGCTTCGTCGGAGTTTCGCCACTTCATTGCCTGTTGTTTGCAGAGAGAGGCTCGGAGAAGATGGACTGCAGCTGCATTACTGGAACATCCGTTCATAACGACGAAAAATGGAGCGAATCAATACCAGAATCAGCCGGCTCATCACCAGAATCTTCGTCAACTGTTACCTCCTCCGCCCCTTCATCCTTCCAGCCTTTCC

mRNA sequence

ATTTGGATTGGATTCTTCTTCTTCTTCATCATCGTCATCAGCGCAGATGAGACCGCTCCAGCCGCCACAGGGTGTGAATCCGACCACCGATCGTCCTCGTCGCCGTCCCAATCTAACTCTTCCTCTTCCGCAGCGGGACAATACGTCTTTGGCGATGGGCCGAGAGGTCAAGATCCTTCGCGACGTTGACAATCCCTATGTGGTCAAGTGCCATGATATGTTCGATCATAACGGGGAAATCCAGGTTCTCCTTGAGTACATGGATCGGGGCTCTTTGGAAGGAACGCATATCCCTCAGGAGCACCATCTCTCTGATTTGGCTCGCCAGATTCTTAGTGGCCTCGCCTATCTCCATAGCCGCCGCATCGTCCATCGCGACATCAAGCCTTCGAATCTCCTCATCAATTCCCGAAGGCAGGTTAAGATCGCTGATTTCGGCGTCGGTCGAATCTTGGAACAGACTATGGATCCTTGCAATTCCTCGGTTGGAACTATTGCGTACATGAGCCCTGAGAGAATCAATTCCGATCTAAATCAGGGCCAGTACAATGGCTATGCTGGGGACATTTGGAGCTTTGGGGTTAGCATATTGGAATTCTATCTCGGACGGTTTCCTCTCGCTGTCGAGAGGCCTGGGGATTGGGCGAGCTTGATGTGCGCTATATGTATGGCTCAGCCACCGGAGGCGCCGCCAACTGCTTCGTCGGAGTTTCGCCACTTCATTGCCTGTTGTTTGCAGAGAGAGGCTCGGAGAAGATGGACTGCAGCTGCATTACTGGAACATCCGTTCATAACGACGAAAAATGGAGCGAATCAATACCAGAATCAGCCGGCTCATCACCAGAATCTTCGTCAACTGTTACCTCCTCCGCCCCTTCATCCTTCCAGCCTTTCC

Coding sequence (CDS)

ATGAGACCGCTCCAGCCGCCACAGGGTGTGAATCCGACCACCGATCGTCCTCGTCGCCGTCCCAATCTAACTCTTCCTCTTCCGCAGCGGGACAATACGTCTTTGGCGATGGGCCGAGAGGTCAAGATCCTTCGCGACGTTGACAATCCCTATGTGGTCAAGTGCCATGATATGTTCGATCATAACGGGGAAATCCAGGTTCTCCTTGAGTACATGGATCGGGGCTCTTTGGAAGGAACGCATATCCCTCAGGAGCACCATCTCTCTGATTTGGCTCGCCAGATTCTTAGTGGCCTCGCCTATCTCCATAGCCGCCGCATCGTCCATCGCGACATCAAGCCTTCGAATCTCCTCATCAATTCCCGAAGGCAGGTTAAGATCGCTGATTTCGGCGTCGGTCGAATCTTGGAACAGACTATGGATCCTTGCAATTCCTCGGTTGGAACTATTGCGTACATGAGCCCTGAGAGAATCAATTCCGATCTAAATCAGGGCCAGTACAATGGCTATGCTGGGGACATTTGGAGCTTTGGGGTTAGCATATTGGAATTCTATCTCGGACGGTTTCCTCTCGCTGTCGAGAGGCCTGGGGATTGGGCGAGCTTGATGTGCGCTATATGTATGGCTCAGCCACCGGAGGCGCCGCCAACTGCTTCGTCGGAGTTTCGCCACTTCATTGCCTGTTGTTTGCAGAGAGAGGCTCGGAGAAGATGGACTGCAGCTGCATTACTGGAACATCCGTTCATAACGACGAAAAATGGAGCGAATCAATACCAGAATCAGCCGGCTCATCACCAGAATCTTCGTCAACTGTTACCTCCTCCGCCCCTTCATCCTTCCAGCCTTTCC

Protein sequence

MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAMGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPPTASSEFRHFIACCLQREARRRWTAAALLEHPFITTKNGANQYQNQPAHHQNLRQLLPPPPLHPSSLS
Homology
BLAST of Cp4.1LG03g14840 vs. ExPASy Swiss-Prot
Match: O80397 (Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK4 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 7.4e-102
Identity = 209/367 (56.95%), Postives = 237/367 (64.58%), Query Frame = 0

Query: 1   MRPLQPPQGVN-PTTDRPRRRPNLTLPLPQRD---------------------------- 60
           MRP+Q P GV+ P   RPRRRP+LTLPLPQRD                            
Sbjct: 1   MRPIQSPPGVSVPVKSRPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGG 60

Query: 61  -------NTSL------------------------------------------------- 120
                  N+S+                                                 
Sbjct: 61  SASSTNTNSSIEAKNYSDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRR 120

Query: 121 AMGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180
            + RE++ILRDV++P VVKCH+MFD NGEIQVLLE+MD+GSLEG H+ +E  L+DL+RQI
Sbjct: 121 QICREIEILRDVNHPNVVKCHEMFDQNGEIQVLLEFMDKGSLEGAHVWKEQQLADLSRQI 180

Query: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240
           LSGLAYLHSR IVHRDIKPSNLLINS + VKIADFGV RIL QTMDPCNSSVGTIAYMSP
Sbjct: 181 LSGLAYLHSRHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSP 240

Query: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 282
           ERIN+DLNQG+Y+GYAGDIWS GVSILEFYLGRFP  V R GDWASLMCAICM+QPPEAP
Sbjct: 241 ERINTDLNQGKYDGYAGDIWSLGVSILEFYLGRFPFPVSRQGDWASLMCAICMSQPPEAP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy Swiss-Prot
Match: Q8RXG3 (Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK5 PE=1 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 1.6e-101
Identity = 200/357 (56.02%), Postives = 228/357 (63.87%), Query Frame = 0

Query: 1   MRPLQPPQGV-NPTTDRPRRRPNLTLPLPQRD---------------------------N 60
           M+P+Q P GV +P  +R R+RP+L+LPLP RD                           N
Sbjct: 1   MKPIQSPSGVASPMKNRLRKRPDLSLPLPHRDVALAVPLPLPPPSSSSSAPASSSAISTN 60

Query: 61  TSLAMG------------------------------------------------REVKIL 120
            S A                                                  RE++IL
Sbjct: 61  ISAAKSLSELERVNRIGSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEIL 120

Query: 121 RDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQILSGLAYLHS 180
           R VD+P VVKCHDMFDHNGEIQVLLE+MD+GSLEG HI QE  L+DL+RQILSGLAYLH 
Sbjct: 121 RSVDHPNVVKCHDMFDHNGEIQVLLEFMDQGSLEGAHIWQEQELADLSRQILSGLAYLHR 180

Query: 181 RRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPERINSDLNQ 240
           R IVHRDIKPSNLLINS + VKIADFGV RIL QTMDPCNSSVGTIAYMSPERIN+DLN 
Sbjct: 181 RHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNH 240

Query: 241 GQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPPTASSEFRH 282
           G+Y+GYAGD+WS GVSILEFYLGRFP AV R GDWASLMCAICM+QPPEAP TAS EFRH
Sbjct: 241 GRYDGYAGDVWSLGVSILEFYLGRFPFAVSRQGDWASLMCAICMSQPPEAPATASQEFRH 300

BLAST of Cp4.1LG03g14840 vs. ExPASy Swiss-Prot
Match: Q9FX43 (Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK9 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.5e-65
Identity = 120/216 (55.56%), Postives = 160/216 (74.07%), Query Frame = 0

Query: 39  REVKILRDVDNPYVVKCHDMFDHN--GEIQVLLEYMDRGSLEGTH-IPQEHHLSDLARQI 98
           RE++ILR  D+PYVVKCH +F+    GE+ +L+EYMD G+LE       E  L+  A+QI
Sbjct: 92  REMEILRRTDSPYVVKCHGIFEKPVVGEVSILMEYMDGGTLESLRGGVTEQKLAGFAKQI 151

Query: 99  LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 158
           L GL+YLH+ +IVHRDIKP+NLL+NS+ +VKIADFGV +IL +++D CNS VGT AYMSP
Sbjct: 152 LKGLSYLHALKIVHRDIKPANLLLNSKNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSP 211

Query: 159 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAV--ERPGDWASLMCAICMAQPPE 218
           ER +S+ + G  + YAGDIWSFG+ +LE  +G FPL    +RP DWA+LMCA+C  +PP 
Sbjct: 212 ERFDSESSGGSSDIYAGDIWSFGLMMLELLVGHFPLLPPGQRP-DWATLMCAVCFGEPPR 271

Query: 219 APPTASSEFRHFIACCLQREARRRWTAAALLEHPFI 250
           AP   S EFR F+ CCL++++ +RWTA  LL HPF+
Sbjct: 272 APEGCSEEFRSFVECCLRKDSSKRWTAPQLLAHPFL 306

BLAST of Cp4.1LG03g14840 vs. ExPASy Swiss-Prot
Match: Q9LPQ3 (Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK7 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.6e-64
Identity = 120/218 (55.05%), Postives = 158/218 (72.48%), Query Frame = 0

Query: 37  MGREVKILRDVDNPYVVKCHDMFDHN--GEIQVLLEYMDRGSLEGTH-IPQEHHLSDLAR 96
           + RE++ILR  D+PYVV+C  +F+    GE+ +L+EYMD G+LE       E  L+  +R
Sbjct: 88  LAREMEILRRTDSPYVVRCQGIFEKPIVGEVSILMEYMDGGNLESLRGAVTEKQLAGFSR 147

Query: 97  QILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYM 156
           QIL GL+YLHS +IVHRDIKP+NLL+NSR +VKIADFGV +I+ +++D CNS VGT AYM
Sbjct: 148 QILKGLSYLHSLKIVHRDIKPANLLLNSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYM 207

Query: 157 SPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAV--ERPGDWASLMCAICMAQP 216
           SPER +S   +   + YAGDIWSFGV ILE ++G FPL    +RP DWA+LMC +C  +P
Sbjct: 208 SPERFDSAAGENS-DVYAGDIWSFGVMILELFVGHFPLLPQGQRP-DWATLMCVVCFGEP 267

Query: 217 PEAPPTASSEFRHFIACCLQREARRRWTAAALLEHPFI 250
           P AP   S EFR F+ CCL++E+  RWTA+ LL HPF+
Sbjct: 268 PRAPEGCSDEFRSFVDCCLRKESSERWTASQLLGHPFL 303

BLAST of Cp4.1LG03g14840 vs. ExPASy Swiss-Prot
Match: Q9M8J5 (Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK8 PE=3 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 1.2e-59
Identity = 110/224 (49.11%), Postives = 163/224 (72.77%), Query Frame = 0

Query: 31  DNTSLAMGREVKILRDVDNPYVVKCHDMFDH-NGEIQVLLEYMDRGSLEGTHIPQEHHLS 90
           D+TSL   RE++ILR V++PYV KCHD+F + +GE+ +L++YMD GSLE      E  L+
Sbjct: 89  DSTSL---REIEILRMVNSPYVAKCHDIFQNPSGEVSILMDYMDLGSLESLRGVTEKQLA 148

Query: 91  DLARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGT 150
            ++RQ+L G  YLH  +IVHRDIKP+NLL +S+ +VKIADFGV +I+ ++++ CNS VGT
Sbjct: 149 LMSRQVLEGKNYLHEHKIVHRDIKPANLLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGT 208

Query: 151 IAYMSPERINSDLN----QGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCA 210
            AYMSPER++S+ +    + + N YAGDIWSFG+++LE  +G +P+      D A+++CA
Sbjct: 209 FAYMSPERLDSEADGVTEEDKSNVYAGDIWSFGLTMLEILVGYYPML----PDQAAIVCA 268

Query: 211 ICMAQPPEAPPTASSEFRHFIACCLQREARRRWTAAALLEHPFI 250
           +C  +PP+AP   S + + F+ CCL+++A  RWTA+ LL HPF+
Sbjct: 269 VCFGEPPKAPEECSDDLKSFMDCCLRKKASERWTASQLLNHPFL 305

BLAST of Cp4.1LG03g14840 vs. NCBI nr
Match: XP_022955876.1 (mitogen-activated protein kinase kinase 5-like [Cucurbita moschata])

HSP 1 Score: 548 bits (1413), Expect = 3.38e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGI 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPDPLPQQHQPFSLADFERVSQIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. NCBI nr
Match: XP_022980656.1 (mitogen-activated protein kinase kinase 5-like [Cucurbita maxima])

HSP 1 Score: 548 bits (1413), Expect = 3.38e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGN 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPNPLPQQHQPFSLADFERVSRIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. NCBI nr
Match: XP_023526826.1 (mitogen-activated protein kinase kinase 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548 bits (1413), Expect = 3.38e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGN 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPDPLPQQHQPFSLADFERVSRIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. NCBI nr
Match: KAG6581804.1 (Mitogen-activated protein kinase kinase 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018255.1 Mitogen-activated protein kinase kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 548 bits (1413), Expect = 3.38e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGI 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPDPLPQQHQPFSLADFERVSRIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. NCBI nr
Match: XP_022949568.1 (mitogen-activated protein kinase kinase 5-like [Cucurbita moschata])

HSP 1 Score: 516 bits (1329), Expect = 2.25e-182
Identity = 271/369 (73.44%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNP TDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPATDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPPSTSQLHNG 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  NRPPEPQPQKHQVFSLADFERVSRIGSGCGGTVYKVIHRPTARVYALKVIYGNHEDAVRL 120

Query: 121 -MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180
            M REV+ILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI
Sbjct: 121 QMCREVEILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180

Query: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240
           LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP
Sbjct: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240

Query: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 283
           ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP
Sbjct: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy TrEMBL
Match: A0A6J1IU66 (mitogen-activated protein kinase kinase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111479957 PE=3 SV=1)

HSP 1 Score: 548 bits (1413), Expect = 1.64e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGN 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPNPLPQQHQPFSLADFERVSRIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy TrEMBL
Match: A0A6J1GV22 (mitogen-activated protein kinase kinase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111457729 PE=3 SV=1)

HSP 1 Score: 548 bits (1413), Expect = 1.64e-195
Identity = 283/367 (77.11%), Postives = 283/367 (77.11%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPSTSQLHNGI 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  RPPDPLPQQHQPFSLADFERVSQIGSGCGGTVYKVIHRPSGRVYALKVIYGNHEDAVRLQ 120

Query: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180
           MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL
Sbjct: 121 MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQIL 180

Query: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240
           SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE
Sbjct: 181 SGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPE 240

Query: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 283
           RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP
Sbjct: 241 RINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy TrEMBL
Match: A0A6J1GCF5 (mitogen-activated protein kinase kinase 5-like OS=Cucurbita moschata OX=3662 GN=LOC111452881 PE=3 SV=1)

HSP 1 Score: 516 bits (1329), Expect = 1.09e-182
Identity = 271/369 (73.44%), Postives = 274/369 (74.25%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNP TDRPRRRPNLTLPLPQRDNTSLA                        
Sbjct: 1   MRPLQPPQGVNPATDRPRRRPNLTLPLPQRDNTSLAVPLPLPPTSSNAAPPPSTSQLHNG 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  NRPPEPQPQKHQVFSLADFERVSRIGSGCGGTVYKVIHRPTARVYALKVIYGNHEDAVRL 120

Query: 121 -MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180
            M REV+ILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI
Sbjct: 121 QMCREVEILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180

Query: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240
           LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP
Sbjct: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240

Query: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 283
           ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP
Sbjct: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy TrEMBL
Match: A0A6J1KDG3 (mitogen-activated protein kinase kinase 5-like OS=Cucurbita maxima OX=3661 GN=LOC111493349 PE=3 SV=1)

HSP 1 Score: 513 bits (1321), Expect = 2.08e-181
Identity = 270/373 (72.39%), Postives = 274/373 (73.46%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRPLQPPQGVNP TDRPRRRPNLTLPLPQRDN+SLA                        
Sbjct: 1   MRPLQPPQGVNPATDRPRRRPNLTLPLPQRDNSSLAVPLPLPPTSSNAAPPPSTSQLHNG 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  NRPPEPQPQPQPQKHQVFSLADFERVSRIGSGCGGTVYKVIHRPTGRVYALKVIYGNHED 120

Query: 121 -----MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDL 180
                M REV+ILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDL
Sbjct: 121 AVRLQMCREVEILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDL 180

Query: 181 ARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIA 240
           ARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIA
Sbjct: 181 ARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIA 240

Query: 241 YMSPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQP 283
           YMSPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQP
Sbjct: 241 YMSPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQP 300

BLAST of Cp4.1LG03g14840 vs. ExPASy TrEMBL
Match: A0A5D3CA27 (Mitogen-activated protein kinase kinase 5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G00560 PE=3 SV=1)

HSP 1 Score: 502 bits (1293), Expect = 3.18e-177
Identity = 263/368 (71.47%), Postives = 269/368 (73.10%), Query Frame = 0

Query: 1   MRPLQPPQGVNPTTDRPRRRPNLTLPLPQRDNTSLA------------------------ 60
           MRP QPP G NP TDR RRRP+L L LPQRDNTSLA                        
Sbjct: 1   MRPFQPPPGTNPPTDRTRRRPHLNLHLPQRDNTSLAVPLPLPPTSSNSAPPPSTSQLHNA 60

Query: 61  ------------------------------------------------------------ 120
                                                                       
Sbjct: 61  NRPPDPHPHQRQPFTLSEFERVSRIGSGCGGTVYKVVHRPTGRVYALKVIYGNHEDAVRL 120

Query: 121 -MGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180
            M REV+ILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEH LSDLARQI
Sbjct: 121 QMCREVEILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHQLSDLARQI 180

Query: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240
           LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP
Sbjct: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240

Query: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 283
           ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP
Sbjct: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 300

BLAST of Cp4.1LG03g14840 vs. TAIR 10
Match: AT1G51660.1 (mitogen-activated protein kinase kinase 4 )

HSP 1 Score: 371.7 bits (953), Expect = 5.2e-103
Identity = 209/367 (56.95%), Postives = 237/367 (64.58%), Query Frame = 0

Query: 1   MRPLQPPQGVN-PTTDRPRRRPNLTLPLPQRD---------------------------- 60
           MRP+Q P GV+ P   RPRRRP+LTLPLPQRD                            
Sbjct: 1   MRPIQSPPGVSVPVKSRPRRRPDLTLPLPQRDVSLAVPLPLPPTSGGSGGSSGSAPSSGG 60

Query: 61  -------NTSL------------------------------------------------- 120
                  N+S+                                                 
Sbjct: 61  SASSTNTNSSIEAKNYSDLVRGNRIGSGAGGTVYKVIHRPSSRLYALKVIYGNHEETVRR 120

Query: 121 AMGREVKILRDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQI 180
            + RE++ILRDV++P VVKCH+MFD NGEIQVLLE+MD+GSLEG H+ +E  L+DL+RQI
Sbjct: 121 QICREIEILRDVNHPNVVKCHEMFDQNGEIQVLLEFMDKGSLEGAHVWKEQQLADLSRQI 180

Query: 181 LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 240
           LSGLAYLHSR IVHRDIKPSNLLINS + VKIADFGV RIL QTMDPCNSSVGTIAYMSP
Sbjct: 181 LSGLAYLHSRHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSP 240

Query: 241 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAP 282
           ERIN+DLNQG+Y+GYAGDIWS GVSILEFYLGRFP  V R GDWASLMCAICM+QPPEAP
Sbjct: 241 ERINTDLNQGKYDGYAGDIWSLGVSILEFYLGRFPFPVSRQGDWASLMCAICMSQPPEAP 300

BLAST of Cp4.1LG03g14840 vs. TAIR 10
Match: AT3G21220.1 (MAP kinase kinase 5 )

HSP 1 Score: 370.5 bits (950), Expect = 1.2e-102
Identity = 200/357 (56.02%), Postives = 228/357 (63.87%), Query Frame = 0

Query: 1   MRPLQPPQGV-NPTTDRPRRRPNLTLPLPQRD---------------------------N 60
           M+P+Q P GV +P  +R R+RP+L+LPLP RD                           N
Sbjct: 1   MKPIQSPSGVASPMKNRLRKRPDLSLPLPHRDVALAVPLPLPPPSSSSSAPASSSAISTN 60

Query: 61  TSLAMG------------------------------------------------REVKIL 120
            S A                                                  RE++IL
Sbjct: 61  ISAAKSLSELERVNRIGSGAGGTVYKVIHTPTSRPFALKVIYGNHEDTVRRQICREIEIL 120

Query: 121 RDVDNPYVVKCHDMFDHNGEIQVLLEYMDRGSLEGTHIPQEHHLSDLARQILSGLAYLHS 180
           R VD+P VVKCHDMFDHNGEIQVLLE+MD+GSLEG HI QE  L+DL+RQILSGLAYLH 
Sbjct: 121 RSVDHPNVVKCHDMFDHNGEIQVLLEFMDQGSLEGAHIWQEQELADLSRQILSGLAYLHR 180

Query: 181 RRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSPERINSDLNQ 240
           R IVHRDIKPSNLLINS + VKIADFGV RIL QTMDPCNSSVGTIAYMSPERIN+DLN 
Sbjct: 181 RHIVHRDIKPSNLLINSAKNVKIADFGVSRILAQTMDPCNSSVGTIAYMSPERINTDLNH 240

Query: 241 GQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCAICMAQPPEAPPTASSEFRH 282
           G+Y+GYAGD+WS GVSILEFYLGRFP AV R GDWASLMCAICM+QPPEAP TAS EFRH
Sbjct: 241 GRYDGYAGDVWSLGVSILEFYLGRFPFAVSRQGDWASLMCAICMSQPPEAPATASQEFRH 300

BLAST of Cp4.1LG03g14840 vs. TAIR 10
Match: AT1G73500.1 (MAP kinase kinase 9 )

HSP 1 Score: 250.4 bits (638), Expect = 1.8e-66
Identity = 120/216 (55.56%), Postives = 160/216 (74.07%), Query Frame = 0

Query: 39  REVKILRDVDNPYVVKCHDMFDHN--GEIQVLLEYMDRGSLEGTH-IPQEHHLSDLARQI 98
           RE++ILR  D+PYVVKCH +F+    GE+ +L+EYMD G+LE       E  L+  A+QI
Sbjct: 92  REMEILRRTDSPYVVKCHGIFEKPVVGEVSILMEYMDGGTLESLRGGVTEQKLAGFAKQI 151

Query: 99  LSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYMSP 158
           L GL+YLH+ +IVHRDIKP+NLL+NS+ +VKIADFGV +IL +++D CNS VGT AYMSP
Sbjct: 152 LKGLSYLHALKIVHRDIKPANLLLNSKNEVKIADFGVSKILVRSLDSCNSYVGTCAYMSP 211

Query: 159 ERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAV--ERPGDWASLMCAICMAQPPE 218
           ER +S+ + G  + YAGDIWSFG+ +LE  +G FPL    +RP DWA+LMCA+C  +PP 
Sbjct: 212 ERFDSESSGGSSDIYAGDIWSFGLMMLELLVGHFPLLPPGQRP-DWATLMCAVCFGEPPR 271

Query: 219 APPTASSEFRHFIACCLQREARRRWTAAALLEHPFI 250
           AP   S EFR F+ CCL++++ +RWTA  LL HPF+
Sbjct: 272 APEGCSEEFRSFVECCLRKDSSKRWTAPQLLAHPFL 306

BLAST of Cp4.1LG03g14840 vs. TAIR 10
Match: AT1G18350.1 (MAP kinase kinase 7 )

HSP 1 Score: 246.5 bits (628), Expect = 2.5e-65
Identity = 120/218 (55.05%), Postives = 158/218 (72.48%), Query Frame = 0

Query: 37  MGREVKILRDVDNPYVVKCHDMFDHN--GEIQVLLEYMDRGSLEGTH-IPQEHHLSDLAR 96
           + RE++ILR  D+PYVV+C  +F+    GE+ +L+EYMD G+LE       E  L+  +R
Sbjct: 88  LAREMEILRRTDSPYVVRCQGIFEKPIVGEVSILMEYMDGGNLESLRGAVTEKQLAGFSR 147

Query: 97  QILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGTIAYM 156
           QIL GL+YLHS +IVHRDIKP+NLL+NSR +VKIADFGV +I+ +++D CNS VGT AYM
Sbjct: 148 QILKGLSYLHSLKIVHRDIKPANLLLNSRNEVKIADFGVSKIITRSLDYCNSYVGTCAYM 207

Query: 157 SPERINSDLNQGQYNGYAGDIWSFGVSILEFYLGRFPLAV--ERPGDWASLMCAICMAQP 216
           SPER +S   +   + YAGDIWSFGV ILE ++G FPL    +RP DWA+LMC +C  +P
Sbjct: 208 SPERFDSAAGENS-DVYAGDIWSFGVMILELFVGHFPLLPQGQRP-DWATLMCVVCFGEP 267

Query: 217 PEAPPTASSEFRHFIACCLQREARRRWTAAALLEHPFI 250
           P AP   S EFR F+ CCL++E+  RWTA+ LL HPF+
Sbjct: 268 PRAPEGCSDEFRSFVDCCLRKESSERWTASQLLGHPFL 303

BLAST of Cp4.1LG03g14840 vs. TAIR 10
Match: AT3G06230.1 (MAP kinase kinase 8 )

HSP 1 Score: 211.5 bits (537), Expect = 9.1e-55
Identity = 102/212 (48.11%), Postives = 153/212 (72.17%), Query Frame = 0

Query: 31  DNTSLAMGREVKILRDVDNPYVVKCHDMFDH-NGEIQVLLEYMDRGSLEGTHIPQEHHLS 90
           D+TSL   RE++ILR V++PYV KCHD+F + +GE+ +L++YMD GSLE      E  L+
Sbjct: 89  DSTSL---REIEILRMVNSPYVAKCHDIFQNPSGEVSILMDYMDLGSLESLRGVTEKQLA 148

Query: 91  DLARQILSGLAYLHSRRIVHRDIKPSNLLINSRRQVKIADFGVGRILEQTMDPCNSSVGT 150
            ++RQ+L G  YLH  +IVHRDIKP+NLL +S+ +VKIADFGV +I+ ++++ CNS VGT
Sbjct: 149 LMSRQVLEGKNYLHEHKIVHRDIKPANLLRSSKEEVKIADFGVSKIVVRSLNKCNSFVGT 208

Query: 151 IAYMSPERINSDLN----QGQYNGYAGDIWSFGVSILEFYLGRFPLAVERPGDWASLMCA 210
            AYMSPER++S+ +    + + N YAGDIWSFG+++LE  +G +P+      D A+++CA
Sbjct: 209 FAYMSPERLDSEADGVTEEDKSNVYAGDIWSFGLTMLEILVGYYPML----PDQAAIVCA 268

Query: 211 ICMAQPPEAPPTASSEFRHFIACCLQREARRR 238
           +C  +PP+AP   S + + F+ CCL+++A  R
Sbjct: 269 VCFGEPPKAPEECSDDLKSFMDCCLRKKASER 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O803977.4e-10256.95Mitogen-activated protein kinase kinase 4 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q8RXG31.6e-10156.02Mitogen-activated protein kinase kinase 5 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9FX432.5e-6555.56Mitogen-activated protein kinase kinase 9 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9LPQ33.6e-6455.05Mitogen-activated protein kinase kinase 7 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9M8J51.2e-5949.11Mitogen-activated protein kinase kinase 8 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
XP_022955876.13.38e-19577.11mitogen-activated protein kinase kinase 5-like [Cucurbita moschata][more]
XP_022980656.13.38e-19577.11mitogen-activated protein kinase kinase 5-like [Cucurbita maxima][more]
XP_023526826.13.38e-19577.11mitogen-activated protein kinase kinase 5-like [Cucurbita pepo subsp. pepo][more]
KAG6581804.13.38e-19577.11Mitogen-activated protein kinase kinase 4, partial [Cucurbita argyrosperma subsp... [more]
XP_022949568.12.25e-18273.44mitogen-activated protein kinase kinase 5-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1IU661.64e-19577.11mitogen-activated protein kinase kinase 5-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GV221.64e-19577.11mitogen-activated protein kinase kinase 5-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1GCF51.09e-18273.44mitogen-activated protein kinase kinase 5-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KDG32.08e-18172.39mitogen-activated protein kinase kinase 5-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5D3CA273.18e-17771.47Mitogen-activated protein kinase kinase 5-like OS=Cucumis melo var. makuwa OX=11... [more]
Match NameE-valueIdentityDescription
AT1G51660.15.2e-10356.95mitogen-activated protein kinase kinase 4 [more]
AT3G21220.11.2e-10256.02MAP kinase kinase 5 [more]
AT1G73500.11.8e-6655.56MAP kinase kinase 9 [more]
AT1G18350.12.5e-6555.05MAP kinase kinase 7 [more]
AT3G06230.19.1e-5548.11MAP kinase kinase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 8..249
e-value: 1.0E-48
score: 177.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 35..249
e-value: 2.0E-50
score: 171.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..249
score: 37.233917
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 75..271
e-value: 4.5E-50
score: 171.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 24..73
e-value: 1.4E-7
score: 33.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR24361MITOGEN-ACTIVATED KINASE KINASE KINASEcoord: 37..264
NoneNo IPR availablePANTHERPTHR24361:SF762MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4coord: 37..264
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 24..250
e-value: 6.78595E-114
score: 326.856
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 107..119
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 37..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g14840.1Cp4.1LG03g14840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity