Cp4.1LG03g12750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g12750
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionhistidine protein methyltransferase 1 homolog
LocationCp4.1LG03: 10040294 .. 10044565 (+)
RNA-Seq ExpressionCp4.1LG03g12750
SyntenyCp4.1LG03g12750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGCACCATCATTGCTAGCCCAGTGCTTGCCTGGCTTGGTGCCCCAAGATAAAGGAAGCCACAGCAGTCCTTCCATTTCAGAGAGAGATGTCCATCTCCCCTCACCAGCTGTGGAGATTCTCCCTTCAAAGGCAATTCATTTATACTTCTCTTCCTGTTATTGCAAAGTACCTCTCATAACTACGAAAAACAATTTTTAGTAATCAAACCTGTATAATCTAGATCGTTTCAATTAACGATTCCATTTGTTTTTTCGTTTTAACGCCTACTTTTGGAATACCATTAACACTTCCTTAACCTTTATCAGACAGCTCTTCCATACAAATATGCAGGGGAGAACGTAGATTTGCAAGGTCTCAATGTCTTTAAGGTACTTCTTTCATTTGTTAGCGGTTATGCCAATTATTCTATATCGTGTGTTCATCGGATAGTTCGAGGCGATTCTTGCTTTTCCTGGATACGATTACTCGAACAAACTATAATGGAACTTTATCAGCAATTCGTAAGCTAAAAACCCCGCAGCTCTTCATTTATGCTAATTGGCGTCGTTCTCTCCAGGGTAGATTTAGCGTTGCCGATATAATGGGCTTGAATAGCTCGGAGTCTACATCTTCAAAACCTGAAGGTACTTTTATACTTTCTACAGTCATAGCTACCTGAGATCTAATTTTCTTTTAAAAATCTGTTTTAGAATCAAGAGTTACCATAACTTTTACGGTTATACATGTGTCGTTAAGGTTGAAATATATCTTTATTATGCAGGTCATTTGAAATCTTGGGACAGTTCCATGTATCTGGTTAATGTTCTTAAATATGAGATTTGTGATGGACAGCTGAGCTTTAGAGGCAAAAAAGTACTTGAGGTTATAATCCATTATAGTAACTTTAAATCGGGCGACCTTATTGTGCATGATTAATTAGATTTCAAAACATTTTGTCTTGTGTAAATGATTGTTCGGGTGTGTTTCTGTAATAGCCCAAGCCCACTACTAGTAGATATTGTTATCTCTGGGCTTTCCCTCAAGATTTTAAAACGTGTTTGCTAGGGAGAGGTTTCCACACCCTTATGAAGAATGTTTCGTTCTTCTCCCCGACTGTTGTGGGATCTCACAGTCCACTCTCCTTTGGGGTCTAGCGTCCTCGCTGACACTCGTTCCCCTCTCCTATCGATGTGGGATCTCCCAATCCACCCTTCTTCAGGATCCAGCGTCTTCGCTGACACTCGTTCCTCTCTCTGATCGACGTGGGATTTCACAATCCACCCCCATTCCGGGCCCAGCGTCCTCGCTGGCACAGAGCCCCGTGTTTGGCTTTGATGCCAACTGTAACGCCCCTAATTTTTTTAGCCAACGGTGGGCTTGGGCTGTTAGTGGATTCCTCATTTCCTTCCCTAAACTTTATGAGATAGAATCTTTTCAAGCTTATCAAAGTGAGGGCCGAAAGGGACGAGGCAGGCACCATTAGTACATTTCGTATTAGTTCTAACGCTGATTTAAGTCATCGTGTTTTGTATCTTGTCGTCTCATTCAAGGAAGGACGACCAAGGCAATCTCTCCGTTACATGTAGGATGCCTTCCTCTCTGTGCATATGTGTTTTTCCCTAACTCTGCTGGCCCAAGCATATTGCTTGAATTATTACTTTATGTTCTATTGAGAACGGTTGCCCTTTCTATTATGCTATGTCAACATGTGGAGCCTTGGAGTGCTCTAATCCACTGAAACAACTAATAACACATCAGTCTGGGGTGTTGCTGATTTCTAAGCTAACCTCTTTAGTTGGTATTTGCAGCTGGGTTGTAGCTATGGACTTCCTGGGGTGTTTGCTTGCCTCAAGGTACCTTTAAACCATCTTTAATCCGTCTCTATACGTATATTCTTCGATAGTAAGCACCATGTCTGATAGTTGTTAAATATGTTTTTCTTACTTCGGTCGCTTATTTTCTTCCTATTATGAGCGTTCTAACTGCGAATTCAATCAAGTAAAGTTGTGTGAAAAAATTTCATTTGAAATGTGCTTGTGGTGTGTAGTTCCTTCGTACAAATTACTCTAAATGTGGAGTTTGAATGGTTTGAGAGTATTCAAATCATCATTGTTATTTATTCACCATTGTTTAATATGTTACAAAAAGATATAATGTAGCCAAGGACTATGAAGAAGCATATGAATACTTTCTTAGATGTTATCAATCCTTACTAAGATTTTGATAAAGATACATTGACATGATTTGTTATAGGGTTTCAACTATTTGTTTTGTTTACGAGCGTTCAACAAGTCTTTGTAGTGTGCTTTCTCGGTGCGTAACTGTAAGATCCCACATCGGTTGGGGAGAAGAATGAAACATTCCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGATGCGTTTTAAAACCGTGAGGTTGACGACGATACGTAATGGGCCAAAACGGACAATATCTGCTAGCGGTGGGTTTGGGCTCTTACAAATGGTATCTGAGTTAGACACGGGGCGATGTGCTAGCTAGGACACTGGGCTCCCAAGAGGGGTGGATTGTGAGATCCCACATCAGTTGGGGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACGCGTTTTAAAACTGTGAGGTTGACGGCGATACGTAACAGACCAAAGCAGACATATTTGCTAGCGGTGGGCTTGGACTGTTATAAATGGTCTCAGAGCTAGACACTGGGCAGTGTGCTAGCGAGGATGCTGGCCACCAAGGGGGTGGATTGTGAGATCCCACATCAGTTGGGGAGGGGAGCGAAGCATATTCCTTATAAAGGGTGTGTAAACCTCATCCTAGTAGACGCGTTTTAAAACCGTGAGGCCGACGACAATATGTAATGGGCTAAAACGGACAATATCTGCTAGCGGTAGGCATAGACTGTTACAAATGGTATTAGAGCCAGACACTGGGTGATGTGCCAGCGAGGACGCTGGTCCTCAAGGGGGGTGGATTGTGAGATCCCACATCGGTCGGGGAGGGGAATGAAGCATTATTTATAAGGGTGTGAAAACCTCTTCCTAGTAGACACCTTTTAAAATTATGAGGCTGACGACGATACGTAACGAGCCAAAGCAGACAATATCTGCTAGCGGTGGGCTTAGAATGCAAGCACAGGAGTAGAAGGTTAATGTTGAAATAGGGAATGGATTTTTGTTGGTTTAGAAGGAAATGAAAGTTGAAGTCTGTTTATTGTAGAATTATATAGAAGAGGTGTCATGGCTTTGCCTTTTATTTTTCAAGGAACTGTTCTTATACCTCAGCTTTCAATCTTCTTACAGGGAGCAAGCATAGTGCACTTTCAAGACATGAGTGCAGAAACTATTAGATGCACAACCATACCGAATGTCCTTGCAAATCTCGAACAAGCTCGGGACAGGCAGAGTCGACAGCCAGAGAGCCCCTTAACTCCGTCCCGACATGCTTTGACCCCATCAGTACATTTTTATGCTGGTGACTGGGAAGAGCTCCCAACAGTCTTATCTGTAGTTCGGGGTGATGGATTCGAAGCACCCACCGGAATGAGCTTGAGCTTCTCAGAAGAAGATTTTATGGATGGTTGCAGCAGTCAAGACGGTAGCATCATCGGCCACGAGTCTTCTTCAAGAAGATCGAGGAAACTATCAGCAAGTCGAGCATGGGAGAGGGCTAACGAGATCGGTCAGGAAGAAGGCGGATACGATATCGTTTTAATGGCAGAAATCCCGTATTCACTCAACTCTTTGAAGAAGCTATATGCACTGATAAAAAAGGTAATTCTCTATACGTCGAAGACTAAAACGAACGTCTAATACCTTTGAATTAATCATCTTTTTTGTTGAAAATCAGTGTGTGAGGCCTCCTTATGGAGTGTTGTACTTGGCCACAAAGAAGAACTACGTCGGTTTCAATAACGGAGCGAGGCATTTGAGGAGTTTGGTCGATGAGGAAGGCGTTTTTGGAGCTCACTTGGTCGAGGAGATGACGGACCGAGAGATTTGGAAGTTCTTTCTCAAGTGAAAGAATGGAAAAACAGGACAAACTGTTGAAGAAGTCCATGTTCATCACAAAGTATATGTTTTTTAAGGAAGTTGTCTTTGTTAGAATGGGAGTTGTTTAGTTAGTGGAGCATTTGTTTTGCTTCTTTGATTGTATAATCTATATTGTTCTTTCTTTCTTTCTTAATTTCTTGTCAATTTTGTGAGGATTCTCCTTGCTATTGCTAGTAAATCAATAATTGAATGCCCTTATTGCCTTTTTTTT

mRNA sequence

ATGAGAGCACCATCATTGCTAGCCCAGTGCTTGCCTGGCTTGGTGCCCCAAGATAAAGGAAGCCACAGCAGTCCTTCCATTTCAGAGAGAGATGTCCATCTCCCCTCACCAGCTGTGGAGATTCTCCCTTCAAAGGCAATTCATTTATACTTCTCTTCCTGTTATTGCAAAACAGCTCTTCCATACAAATATGCAGGGGAGAACGTAGATTTGCAAGGTCTCAATGTCTTTAAGGGTAGATTTAGCGTTGCCGATATAATGGGCTTGAATAGCTCGGAGTCTACATCTTCAAAACCTGAAGGTCATTTGAAATCTTGGGACAGTTCCATGTATCTGGTTAATGTTCTTAAATATGAGATTTGTGATGGACAGCTGAGCTTTAGAGGCAAAAAAGTACTTGAGCTGGGTTGTAGCTATGGACTTCCTGGGGTGTTTGCTTGCCTCAAGGGAGCAAGCATAGTGCACTTTCAAGACATGAGTGCAGAAACTATTAGATGCACAACCATACCGAATGTCCTTGCAAATCTCGAACAAGCTCGGGACAGGCAGAGTCGACAGCCAGAGAGCCCCTTAACTCCGTCCCGACATGCTTTGACCCCATCAGTACATTTTTATGCTGGTGACTGGGAAGAGCTCCCAACAGTCTTATCTGTAGTTCGGGGTGATGGATTCGAAGCACCCACCGGAATGAGCTTGAGCTTCTCAGAAGAAGATTTTATGGATGGTTGCAGCAGTCAAGACGGTAGCATCATCGGCCACGAGTCTTCTTCAAGAAGATCGAGGAAACTATCAGCAAGTCGAGCATGGGAGAGGGCTAACGAGATCGGTCAGGAAGAAGGCGGATACGATATCGTTTTAATGGCAGAAATCCCGTATTCACTCAACTCTTTGAAGAAGCTATATGCACTGATAAAAAAGTGTGTGAGGCCTCCTTATGGAGTGTTGTACTTGGCCACAAAGAAGAACTACGTCGGTTTCAATAACGGAGCGAGGCATTTGAGGAGTTTGGTCGATGAGGAAGGCGTTTTTGGAGCTCACTTGGTCGAGGAGATGACGGACCGAGAGATTTGGAAGTTCTTTCTCAAGTGAAAGAATGGAAAAACAGGACAAACTGTTGAAGAAGTCCATGTTCATCACAAAGTATATGTTTTTTAAGGAAGTTGTCTTTGTTAGAATGGGAGTTGTTTAGTTAGTGGAGCATTTGTTTTGCTTCTTTGATTGTATAATCTATATTGTTCTTTCTTTCTTTCTTAATTTCTTGTCAATTTTGTGAGGATTCTCCTTGCTATTGCTAGTAAATCAATAATTGAATGCCCTTATTGCCTTTTTTTT

Coding sequence (CDS)

ATGAGAGCACCATCATTGCTAGCCCAGTGCTTGCCTGGCTTGGTGCCCCAAGATAAAGGAAGCCACAGCAGTCCTTCCATTTCAGAGAGAGATGTCCATCTCCCCTCACCAGCTGTGGAGATTCTCCCTTCAAAGGCAATTCATTTATACTTCTCTTCCTGTTATTGCAAAACAGCTCTTCCATACAAATATGCAGGGGAGAACGTAGATTTGCAAGGTCTCAATGTCTTTAAGGGTAGATTTAGCGTTGCCGATATAATGGGCTTGAATAGCTCGGAGTCTACATCTTCAAAACCTGAAGGTCATTTGAAATCTTGGGACAGTTCCATGTATCTGGTTAATGTTCTTAAATATGAGATTTGTGATGGACAGCTGAGCTTTAGAGGCAAAAAAGTACTTGAGCTGGGTTGTAGCTATGGACTTCCTGGGGTGTTTGCTTGCCTCAAGGGAGCAAGCATAGTGCACTTTCAAGACATGAGTGCAGAAACTATTAGATGCACAACCATACCGAATGTCCTTGCAAATCTCGAACAAGCTCGGGACAGGCAGAGTCGACAGCCAGAGAGCCCCTTAACTCCGTCCCGACATGCTTTGACCCCATCAGTACATTTTTATGCTGGTGACTGGGAAGAGCTCCCAACAGTCTTATCTGTAGTTCGGGGTGATGGATTCGAAGCACCCACCGGAATGAGCTTGAGCTTCTCAGAAGAAGATTTTATGGATGGTTGCAGCAGTCAAGACGGTAGCATCATCGGCCACGAGTCTTCTTCAAGAAGATCGAGGAAACTATCAGCAAGTCGAGCATGGGAGAGGGCTAACGAGATCGGTCAGGAAGAAGGCGGATACGATATCGTTTTAATGGCAGAAATCCCGTATTCACTCAACTCTTTGAAGAAGCTATATGCACTGATAAAAAAGTGTGTGAGGCCTCCTTATGGAGTGTTGTACTTGGCCACAAAGAAGAACTACGTCGGTTTCAATAACGGAGCGAGGCATTTGAGGAGTTTGGTCGATGAGGAAGGCGTTTTTGGAGCTCACTTGGTCGAGGAGATGACGGACCGAGAGATTTGGAAGTTCTTTCTCAAGTGA

Protein sequence

MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTALPYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQARDRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFMDGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKLYALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFFLK
Homology
BLAST of Cp4.1LG03g12750 vs. ExPASy Swiss-Prot
Match: Q4KM84 (Histidine protein methyltransferase 1 homolog OS=Rattus norvegicus OX=10116 GN=Mettl18 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-12
Identity = 81/352 (23.01%), Postives = 134/352 (38.07%), Query Frame = 0

Query: 25  PSISERDVH-LPSPAVEILPSKAIHLYFSSCYCKTA------LPYKYAGENVDLQGLNVF 84
           PS+     H +P    ++L +K + +   S +  TA      L  K+ GEN+  Q  +  
Sbjct: 85  PSLKPAKEHVIPKDCDQVLENKVLEMLPGSQHVSTAVVKTISLKEKFPGENIVSQSFS-- 144

Query: 85  KGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVLELGC 144
                       + S+      EG LK W+ +  L+          ++ F G+KVL+LGC
Sbjct: 145 ------------SHSDLIPGVYEGGLKIWECTFDLMTY----FTKAKVKFAGQKVLDLGC 204

Query: 145 SYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQARDRQ-------SRQPESP 204
             GL G+ A   GA  VHFQD +   I   T+PNV+AN+    D          RQ +S 
Sbjct: 205 GSGLLGITASKGGAREVHFQDYNGLVIDEVTLPNVVANVPLQGDSNGINEPAGKRQRKSE 264

Query: 205 LTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFMDGCSSQDGSI 264
           +       T     ++G+W E                    L  SE+ F+          
Sbjct: 265 VAQE----TCKCRLFSGEWAEF-----------------CKLVLSEKLFVK--------- 324

Query: 265 IGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKLYALIKKCVRP 324
                                          YD++L +E  Y+ +    L+  + + +  
Sbjct: 325 -------------------------------YDLILTSETIYNPDYYSTLHETLLRLL-S 355

Query: 325 PYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFFLK 363
             G + LA+K +Y G   G    +  V+E+GVF    + E+ D  + +F ++
Sbjct: 385 RNGRVLLASKAHYFGVGGGVHLFQKFVEEKGVFETRTL-EVIDEGLKRFLME 355

BLAST of Cp4.1LG03g12750 vs. ExPASy Swiss-Prot
Match: Q2KIJ2 (Histidine protein methyltransferase 1 homolog OS=Bos taurus OX=9913 GN=METTL18 PE=2 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.4e-11
Identity = 67/289 (23.18%), Postives = 115/289 (39.79%), Query Frame = 0

Query: 68  NVDLQGLNVFKGRFSVADIMG---LNSSESTSSKPEGHLKSWDSSMYLVNVLKYEICDGQ 127
           N+ +    + K  F   +I+     + S+  S   EG LK W+ +    ++L Y +   +
Sbjct: 128 NISIMKTTLLKENFPGENIISKSFSSHSDLISGVYEGGLKIWECTF---DLLAY-LTKAK 187

Query: 128 LSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQARDRQS 187
           + F GKKVL+LGC  GL G+ A   GA  +HFQD ++  I   T+PNV+AN     D ++
Sbjct: 188 VKFAGKKVLDLGCGSGLLGIMALKGGAKEIHFQDYNSVVIDEVTLPNVVAN-STLEDEEN 247

Query: 188 RQPESPLTPSRHALTP----SVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 247
              E  +   R +          F++G+W E   ++                        
Sbjct: 248 DVNEPDVKRLRRSTVAQELCKCRFFSGEWSEFCKLV------------------------ 307

Query: 248 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 307
                                 LS+ + +E+          YD++L +E  Y+ +    L
Sbjct: 308 ----------------------LSSEKLFEK----------YDLILTSETIYNPDYYVPL 354

Query: 308 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVE 350
           +    + +    G + LA+K +Y G   G    +  V+E  VF    +E
Sbjct: 368 HQTFLRLL-DKNGQVLLASKVHYFGVGGGTHLFQKFVEERNVFETRTLE 354

BLAST of Cp4.1LG03g12750 vs. ExPASy Swiss-Prot
Match: Q55DL2 (Histidine protein methyltransferase 1 homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0270580 PE=3 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 2.2e-10
Identity = 36/76 (47.37%), Postives = 47/76 (61.84%), Query Frame = 0

Query: 100 EGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDM 159
           EG  K W+ S+ ++N L  E    ++   GKKVLE+GC +GLPG++ CL   SIV FQD 
Sbjct: 127 EGGFKLWECSIDIINYLFEE----KIDLSGKKVLEIGCGHGLPGIY-CLLNGSIVTFQDY 186

Query: 160 SAETIRCTTIPNVLAN 176
           + E I   T PNVL N
Sbjct: 187 NEEVIYNLTQPNVLIN 197

BLAST of Cp4.1LG03g12750 vs. ExPASy Swiss-Prot
Match: Q9CZ09 (Histidine protein methyltransferase 1 homolog OS=Mus musculus OX=10090 GN=Mettl18 PE=2 SV=2)

HSP 1 Score: 67.0 bits (162), Expect = 5.0e-10
Identity = 78/342 (22.81%), Postives = 135/342 (39.47%), Query Frame = 0

Query: 27  ISERDVH--LPSPAVEILPSKAIHLYFSSCYCKT-ALPYKYAGENVDLQGLNVFKGRFSV 86
           +  +DV+  L +  +E+LP      + ++   KT +L  K+ GEN+      V K   S 
Sbjct: 94  VMPKDVNHVLENTVLEMLPGPQ---HANTAVVKTVSLKEKFPGENI------VSKSFSSH 153

Query: 87  ADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVLELGCSYGLPG 146
           +D++            EG LK W+ +    ++L Y     ++ F G+KVL+LGC  GL G
Sbjct: 154 SDLI--------PGVYEGGLKIWECTF---DLLTY-FTKAKVKFAGQKVLDLGCGSGLLG 213

Query: 147 VFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQARDRQSRQPESPLTPSRHALTPSV- 206
           + A   GA  VHFQD +   I   T+PNV+AN+    D   +         +  +   + 
Sbjct: 214 ITASKGGAREVHFQDYNGLVIDEVTLPNVVANVPLQDDSNGKNEPDGKRQRKSEVGREIC 273

Query: 207 --HFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFMDGCSSQDGSIIGHESSSRRS 266
               ++G+W E                    L   E+ F+                    
Sbjct: 274 KCRLFSGEWAEF-----------------CKLVLREKLFVK------------------- 333

Query: 267 RKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKLYALIKKCVRPPYGVLYLATK 326
                                YD++L +E  Y+ +    L+    + +    G + LA+K
Sbjct: 334 ---------------------YDLILTSETIYNPDYYSTLHETFLRLLSRS-GRVLLASK 355

Query: 327 KNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFFLK 363
            +Y G   G    +  V+E+GVF    + E+ D  + +F ++
Sbjct: 394 AHYFGVGGGVHLFQKFVEEKGVFETRTL-EVIDEGLKRFLME 355

BLAST of Cp4.1LG03g12750 vs. ExPASy Swiss-Prot
Match: O95568 (Histidine protein methyltransferase 1 homolog OS=Homo sapiens OX=9606 GN=METTL18 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.2e-09
Identity = 39/115 (33.91%), Postives = 61/115 (53.04%), Query Frame = 0

Query: 100 EGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDM 159
           EG LK W+ +    ++L Y     ++ F GKKVL+LGC  GL G+ A   G+  +HFQD 
Sbjct: 163 EGGLKIWECTF---DLLAY-FTKAKVKFAGKKVLDLGCGSGLLGITAFKGGSKEIHFQDY 222

Query: 160 SAETIRCTTIPNVLANLEQARDRQSRQPESPLTPSRHALTPSVH---FYAGDWEE 212
           ++  I   T+PNV+AN     D ++   E  +   R      ++   F++G+W E
Sbjct: 223 NSMVIDEVTLPNVVAN-STLEDEENDVNEPDVKRCRKPKVTQLYKCRFFSGEWSE 272

BLAST of Cp4.1LG03g12750 vs. NCBI nr
Match: XP_022956190.1 (histidine protein methyltransferase 1 homolog isoform X1 [Cucurbita moschata])

HSP 1 Score: 729 bits (1883), Expect = 1.63e-265
Identity = 361/362 (99.72%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGA+HLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGAKHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360

Query: 361 LK 362
           LK
Sbjct: 361 LK 362

BLAST of Cp4.1LG03g12750 vs. NCBI nr
Match: XP_022979834.1 (histidine protein methyltransferase 1 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 727 bits (1876), Expect = 1.91e-264
Identity = 359/362 (99.17%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERD+HLPSPAVEILPSKAIHLYFSSCYCKTAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDIHLPSPAVEILPSKAIHLYFSSCYCKTAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRC TIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCITIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDR+IWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDRDIWKFF 360

Query: 361 LK 362
           LK
Sbjct: 361 LK 362

BLAST of Cp4.1LG03g12750 vs. NCBI nr
Match: XP_023529042.1 (histidine protein methyltransferase 1 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694 bits (1792), Expect = 7.74e-252
Identity = 350/362 (96.69%), Postives = 350/362 (96.69%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK            TAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK------------TAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 350

Query: 361 LK 362
           LK
Sbjct: 361 LK 350

BLAST of Cp4.1LG03g12750 vs. NCBI nr
Match: XP_022956191.1 (histidine protein methyltransferase 1 homolog isoform X2 [Cucurbita moschata] >KAG7018474.1 Histidine protein methyltransferase 1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 693 bits (1789), Expect = 2.22e-251
Identity = 349/362 (96.41%), Postives = 350/362 (96.69%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK            TAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK------------TAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGA+HLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGAKHLRSLVDEEGVFGAHLVEEMTDREIWKFF 350

Query: 361 LK 362
           LK
Sbjct: 361 LK 350

BLAST of Cp4.1LG03g12750 vs. NCBI nr
Match: KAG6582044.1 (Histidine protein methyltransferase 1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1789), Expect = 7.65e-251
Identity = 349/362 (96.41%), Postives = 350/362 (96.69%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK            TAL
Sbjct: 34  MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK------------TAL 93

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 94  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 153

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 154 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 213

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 214 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 273

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 274 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 333

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGA+HLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 334 YALIKKCVRPPYGVLYLATKKNYVGFNNGAKHLRSLVDEEGVFGAHLVEEMTDREIWKFF 383

Query: 361 LK 362
           LK
Sbjct: 394 LK 383

BLAST of Cp4.1LG03g12750 vs. ExPASy TrEMBL
Match: A0A6J1GX49 (histidine protein methyltransferase 1 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457960 PE=4 SV=1)

HSP 1 Score: 729 bits (1883), Expect = 7.91e-266
Identity = 361/362 (99.72%), Postives = 362/362 (100.00%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGA+HLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGAKHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360

Query: 361 LK 362
           LK
Sbjct: 361 LK 362

BLAST of Cp4.1LG03g12750 vs. ExPASy TrEMBL
Match: A0A6J1IXP6 (histidine protein methyltransferase 1 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479415 PE=4 SV=1)

HSP 1 Score: 727 bits (1876), Expect = 9.23e-265
Identity = 359/362 (99.17%), Postives = 361/362 (99.72%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERD+HLPSPAVEILPSKAIHLYFSSCYCKTAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDIHLPSPAVEILPSKAIHLYFSSCYCKTAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRC TIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCITIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDR+IWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDRDIWKFF 360

Query: 361 LK 362
           LK
Sbjct: 361 LK 362

BLAST of Cp4.1LG03g12750 vs. ExPASy TrEMBL
Match: A0A6J1GVW0 (histidine protein methyltransferase 1 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457960 PE=4 SV=1)

HSP 1 Score: 693 bits (1789), Expect = 1.07e-251
Identity = 349/362 (96.41%), Postives = 350/362 (96.69%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK            TAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSK------------TAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGA+HLRSLVDEEGVFGAHLVEEMTDREIWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGAKHLRSLVDEEGVFGAHLVEEMTDREIWKFF 350

Query: 361 LK 362
           LK
Sbjct: 361 LK 350

BLAST of Cp4.1LG03g12750 vs. ExPASy TrEMBL
Match: A0A6J1IRW9 (histidine protein methyltransferase 1 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479415 PE=4 SV=1)

HSP 1 Score: 691 bits (1782), Expect = 1.25e-250
Identity = 347/362 (95.86%), Postives = 349/362 (96.41%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGLVPQDKGSHSSPSISERD+HLPSPAVEILPSK            TAL
Sbjct: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDIHLPSPAVEILPSK------------TAL 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
           CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRC TIPNVLANLEQAR
Sbjct: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCITIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDR+IWKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDRDIWKFF 350

Query: 361 LK 362
           LK
Sbjct: 361 LK 350

BLAST of Cp4.1LG03g12750 vs. ExPASy TrEMBL
Match: A0A1S3BXP6 (uncharacterized protein LOC103494545 OS=Cucumis melo OX=3656 GN=LOC103494545 PE=4 SV=1)

HSP 1 Score: 647 bits (1668), Expect = 2.96e-233
Identity = 322/362 (88.95%), Postives = 337/362 (93.09%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLL+QCLPGL+PQDKGSHSSPSISERDVHLPSPAVEILPSK  H            
Sbjct: 1   MRAPSLLSQCLPGLLPQDKGSHSSPSISERDVHLPSPAVEILPSKTAH------------ 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
           PYKYAGENVDLQGLNVFKGR SVADI+G N SESTSSKPEGHLKSWDSS+ LVNVLK+EI
Sbjct: 61  PYKYAGENVDLQGLNVFKGRVSVADIIGFNGSESTSSKPEGHLKSWDSSIDLVNVLKHEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
            DGQLSFRGK+VLELGCSYGLPGVFACLKGASIVHFQD+SAET+RCTTIPNVLANLEQAR
Sbjct: 121 RDGQLSFRGKRVLELGCSYGLPGVFACLKGASIVHFQDLSAETVRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240
           DRQSRQPESPLTPSRH L PSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM
Sbjct: 181 DRQSRQPESPLTPSRHTLAPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFM 240

Query: 241 DGCSSQDGSIIGHESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKL 300
           DGCSSQDGSIIGHESSSRRSRKLS SRAWERA+E  Q EGGYDI+LMAEIP+SLNSLKKL
Sbjct: 241 DGCSSQDGSIIGHESSSRRSRKLSGSRAWERASEADQGEGGYDIILMAEIPFSLNSLKKL 300

Query: 301 YALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIWKFF 360
           YALIKKCVRPPYGVLYLATKKNYVGFN+GARHLRSLVDEEGVFGAHLV+EMTDR++WKFF
Sbjct: 301 YALIKKCVRPPYGVLYLATKKNYVGFNSGARHLRSLVDEEGVFGAHLVKEMTDRDVWKFF 350

Query: 361 LK 362
           LK
Sbjct: 361 LK 350

BLAST of Cp4.1LG03g12750 vs. TAIR 10
Match: AT2G43320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 512.7 bits (1319), Expect = 2.4e-145
Identity = 261/365 (71.51%), Postives = 305/365 (83.56%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGL+PQD+G  S  ++SE+D+ LP+PAVEI+PS            KT  
Sbjct: 1   MRAPSLLAQCLPGLLPQDRGGVS--ALSEKDLQLPTPAVEIIPS------------KTVA 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
            ++Y+GEN+D  GL VFKG+ SVADI+GL+ SE+   K EG LKSW+SS+ LVNVLK EI
Sbjct: 61  HHRYSGENLDALGLQVFKGKVSVADIIGLSGSETAPLKNEGSLKSWESSVVLVNVLKNEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
            DGQLSFRGK+VLELGC++G+PG+FACLKGAS VHFQD+SAETIRCTTIPNVLANLEQAR
Sbjct: 121 RDGQLSFRGKRVLELGCNFGVPGIFACLKGASSVHFQDLSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFE-APTGMSLSFSEEDF 240
           DRQSRQPESPLTPSR A++ SV FYAG+WEEL TVLS++R D  E +   M+LSFSEEDF
Sbjct: 181 DRQSRQPESPLTPSRQAISASVRFYAGEWEELSTVLSIIRTDVLEPSIPAMNLSFSEEDF 240

Query: 241 MDGCSSQDGSIIGH-ESSSRRSRKLSASRAWERANEIGQE-EGGYDIVLMAEIPYSLNSL 300
           MDGCSSQDGSI G  + SSRRSRKLS SRAWERANE GQE E GYD++LM EIPYS+ SL
Sbjct: 241 MDGCSSQDGSITGQPDFSSRRSRKLSGSRAWERANETGQEGEYGYDVILMTEIPYSITSL 300

Query: 301 KKLYALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIW 360
           KKLY+LIKKC+RPPYGV+YLA KK YVGFN+GA+HLR+LVDEE + GAHLV+E TDR+IW
Sbjct: 301 KKLYSLIKKCLRPPYGVMYLAAKKQYVGFNSGAKHLRNLVDEETILGAHLVKETTDRDIW 351

Query: 361 KFFLK 363
           KFFLK
Sbjct: 361 KFFLK 351

BLAST of Cp4.1LG03g12750 vs. TAIR 10
Match: AT2G43320.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 512.7 bits (1319), Expect = 2.4e-145
Identity = 261/365 (71.51%), Postives = 305/365 (83.56%), Query Frame = 0

Query: 1   MRAPSLLAQCLPGLVPQDKGSHSSPSISERDVHLPSPAVEILPSKAIHLYFSSCYCKTAL 60
           MRAPSLLAQCLPGL+PQD+G  S  ++SE+D+ LP+PAVEI+PS            KT  
Sbjct: 1   MRAPSLLAQCLPGLLPQDRGGVS--ALSEKDLQLPTPAVEIIPS------------KTVA 60

Query: 61  PYKYAGENVDLQGLNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEI 120
            ++Y+GEN+D  GL VFKG+ SVADI+GL+ SE+   K EG LKSW+SS+ LVNVLK EI
Sbjct: 61  HHRYSGENLDALGLQVFKGKVSVADIIGLSGSETAPLKNEGSLKSWESSVVLVNVLKNEI 120

Query: 121 CDGQLSFRGKKVLELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQAR 180
            DGQLSFRGK+VLELGC++G+PG+FACLKGAS VHFQD+SAETIRCTTIPNVLANLEQAR
Sbjct: 121 RDGQLSFRGKRVLELGCNFGVPGIFACLKGASSVHFQDLSAETIRCTTIPNVLANLEQAR 180

Query: 181 DRQSRQPESPLTPSRHALTPSVHFYAGDWEELPTVLSVVRGDGFE-APTGMSLSFSEEDF 240
           DRQSRQPESPLTPSR A++ SV FYAG+WEEL TVLS++R D  E +   M+LSFSEEDF
Sbjct: 181 DRQSRQPESPLTPSRQAISASVRFYAGEWEELSTVLSIIRTDVLEPSIPAMNLSFSEEDF 240

Query: 241 MDGCSSQDGSIIGH-ESSSRRSRKLSASRAWERANEIGQE-EGGYDIVLMAEIPYSLNSL 300
           MDGCSSQDGSI G  + SSRRSRKLS SRAWERANE GQE E GYD++LM EIPYS+ SL
Sbjct: 241 MDGCSSQDGSITGQPDFSSRRSRKLSGSRAWERANETGQEGEYGYDVILMTEIPYSITSL 300

Query: 301 KKLYALIKKCVRPPYGVLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTDREIW 360
           KKLY+LIKKC+RPPYGV+YLA KK YVGFN+GA+HLR+LVDEE + GAHLV+E TDR+IW
Sbjct: 301 KKLYSLIKKCLRPPYGVMYLAAKKQYVGFNSGAKHLRNLVDEETILGAHLVKETTDRDIW 351

Query: 361 KFFLK 363
           KFFLK
Sbjct: 361 KFFLK 351

BLAST of Cp4.1LG03g12750 vs. TAIR 10
Match: AT4G14000.1 (Putative methyltransferase family protein )

HSP 1 Score: 181.4 bits (459), Expect = 1.3e-45
Identity = 104/294 (35.37%), Postives = 145/294 (49.32%), Query Frame = 0

Query: 74  LNVFKGRFSVADIMGLNSSESTSSKPEGHLKSWDSSMYLVNVLKYEICDGQLSFRGKKVL 133
           + + KGR S  ++ GL +S+      EG LK W+ S+ LV  L+ E   G LSF GK+VL
Sbjct: 60  ITLLKGRVSTKEVFGLPNSDLVPGVYEGGLKLWEGSIDLVKALEKESQTGNLSFSGKRVL 119

Query: 134 ELGCSYGLPGVFACLKGASIVHFQDMSAETIRCTTIPNVLANLEQARDRQSRQPESPLTP 193
           ELGC + LPG++ACLKG+  VHFQD +AE +RC TIPN+ ANL            S  + 
Sbjct: 120 ELGCGHALPGIYACLKGSDAVHFQDFNAEVLRCLTIPNLNANL------------SEKSS 179

Query: 194 SRHALTPSVHFYAGDWEELPTVLSVVRGDGFEAPTGMSLSFSEEDFMDGCSSQDGSIIGH 253
           S       V F+AG+W E+  VL +V  DG                              
Sbjct: 180 SVSVSETEVRFFAGEWSEVHQVLPLVNSDGET---------------------------- 239

Query: 254 ESSSRRSRKLSASRAWERANEIGQEEGGYDIVLMAEIPYSLNSLKKLYALIKKCVRPPYG 313
                                   ++GGYDI+LMAE  YS+++ K  Y LIK+C+  P G
Sbjct: 240 -----------------------NKKGGYDIILMAETIYSISAQKSQYELIKRCLAYPDG 290

Query: 314 VLYLATKKNYVGFNNGARHLRSLVDEEGVFGAHLVEEMTD-----REIWKFFLK 363
            +Y+A KK Y G   G R   S+++++G   + LV ++TD     RE+WK   K
Sbjct: 300 AVYMAAKKYYFGVGGGTRQFLSMIEKDGALASTLVSQVTDGSSNVREVWKLSYK 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q4KM845.3e-1223.01Histidine protein methyltransferase 1 homolog OS=Rattus norvegicus OX=10116 GN=M... [more]
Q2KIJ23.4e-1123.18Histidine protein methyltransferase 1 homolog OS=Bos taurus OX=9913 GN=METTL18 P... [more]
Q55DL22.2e-1047.37Histidine protein methyltransferase 1 homolog OS=Dictyostelium discoideum OX=446... [more]
Q9CZ095.0e-1022.81Histidine protein methyltransferase 1 homolog OS=Mus musculus OX=10090 GN=Mettl1... [more]
O955684.2e-0933.91Histidine protein methyltransferase 1 homolog OS=Homo sapiens OX=9606 GN=METTL18... [more]
Match NameE-valueIdentityDescription
XP_022956190.11.63e-26599.72histidine protein methyltransferase 1 homolog isoform X1 [Cucurbita moschata][more]
XP_022979834.11.91e-26499.17histidine protein methyltransferase 1 homolog isoform X1 [Cucurbita maxima][more]
XP_023529042.17.74e-25296.69histidine protein methyltransferase 1 homolog [Cucurbita pepo subsp. pepo][more]
XP_022956191.12.22e-25196.41histidine protein methyltransferase 1 homolog isoform X2 [Cucurbita moschata] >K... [more]
KAG6582044.17.65e-25196.41Histidine protein methyltransferase 1-like protein, partial [Cucurbita argyrospe... [more]
Match NameE-valueIdentityDescription
A0A6J1GX497.91e-26699.72histidine protein methyltransferase 1 homolog isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1IXP69.23e-26599.17histidine protein methyltransferase 1 homolog isoform X1 OS=Cucurbita maxima OX=... [more]
A0A6J1GVW01.07e-25196.41histidine protein methyltransferase 1 homolog isoform X2 OS=Cucurbita moschata O... [more]
A0A6J1IRW91.25e-25095.86histidine protein methyltransferase 1 homolog isoform X2 OS=Cucurbita maxima OX=... [more]
A0A1S3BXP62.96e-23388.95uncharacterized protein LOC103494545 OS=Cucumis melo OX=3656 GN=LOC103494545 PE=... [more]
Match NameE-valueIdentityDescription
AT2G43320.12.4e-14571.51S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G43320.22.4e-14571.51S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G14000.11.3e-4535.37Putative methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 87..340
e-value: 1.7E-24
score: 88.4
NoneNo IPR availablePANTHERPTHR14614:SF43OS09G0514300 PROTEINcoord: 1..358
IPR019410Lysine methyltransferasePANTHERPTHR14614HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGENcoord: 1..358
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 116..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g12750.1Cp4.1LG03g12750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity