Cp4.1LG03g11280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g11280 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.8e-62
Identity = 159/442 (35.97%), Postives = 235/442 (53.17%), Query Frame = 0

Query: 5   LSLTILES--TARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNY 64
           LSL +L S   +      ++GFTA LIHRDSP SPFY+ + T++ ++   +HRS +R+  
Sbjct: 10  LSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV-- 69

Query: 65  LYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCN 124
             ++   K   DN       L    GEYLM+ +IG PP  +M  ADT + L+W QC+ C+
Sbjct: 70  --FHFTEK---DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCD 129

Query: 125 SQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETS 184
             C  +  P   F P  S TY+   C S+ C +L    +C+++D  C Y L Y DNS T 
Sbjct: 130 -DCYTQVDPL--FDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 189

Query: 185 GNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIK- 244
           GN++ D+ +  ++D + + +  +  GC         +   G VGL   P+SLI QLG   
Sbjct: 190 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 249

Query: 245 --KFSYCLVPF--NLGSTSKMYFGSLPVTSGG---QTPLLYPNSDA--YYVKVLGISVGA 304
             KFSYCLVP       TSK+ FG+  + SG     TPL+   S    YY+ +  ISVG+
Sbjct: 250 DGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGS 309

Query: 305 DDPNLDGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFEL 364
                 G  D        IIDSGTT + L T+ +  L     +  D  +KK+DP++   L
Sbjct: 310 KQIQYSG-SDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSID-AEKKQDPQSGLSL 369

Query: 365 CFAANANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQL 424
           C++A   D++  P +T+H DGA++ L+  + FV++ +D ++C A  R     SI GN   
Sbjct: 370 CYSA-TGDLKV-PVITMHFDGADVKLDSSNAFVQVSED-LVCFA-FRGSPSFSIYGNVAQ 429

Query: 425 QNYHVGYDLEAQVVSFAPVNCA 435
            N+ VGYD  ++ VSF P +CA
Sbjct: 430 MNFLVGYDTVSKTVSFKPTDCA 435

BLAST of Cp4.1LG03g11280 vs. ExPASy Swiss-Prot
Match: Q3EBM5 (Probable aspartic protease At2g35615 OS=Arabidopsis thaliana OX=3702 GN=At2g35615 PE=3 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.7e-55
Identity = 164/461 (35.57%), Postives = 237/461 (51.41%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           + FFL  ++  S++ H       F+  LIHRDSP+SP Y+  +T T ++ A   RS SR 
Sbjct: 7   LCFFLFFSVTLSSSGH----PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSR- 66

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           +  + + LS+  L +       L+   GE+ MS  IG PP +V   ADT + L WVQC  
Sbjct: 67  SRRFNHQLSQTDLQSG------LIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 126

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQT-CNSSDRWCKYRLVYEDNS 180
           C  QC  E GP   F   KS TY+ EPC S  C +L+  +  C+ S+  CKYR  Y D S
Sbjct: 127 C-QQCYKENGPI--FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQS 186

Query: 181 ETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGS--VGLNQTPLSLISQ 240
            + G++++++ S D+  G  V      FGC      GG     GS  +GL    LSLISQ
Sbjct: 187 FSKGDVATETVSIDSASGSPVSFPGTVFGCGYN--NGGTFDETGSGIIGLGGGHLSLISQ 246

Query: 241 LG---IKKFSYCL----------VPFNLGSTSKMYFGSLPVTSG-GQTPLLYPNSDAYYV 300
           LG    KKFSYCL             NLG+ S     SL   SG   TPL+      YY 
Sbjct: 247 LGSSISKKFSYCLSHKSATTNGTSVINLGTNS--IPSSLSKDSGVVSTPLVDKEPLTYYY 306

Query: 301 KVL-GISVG---------ADDPNLDGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF 360
             L  ISVG         + +PN DG+    +     IIDSGTT + LE   FD+  +  
Sbjct: 307 LTLEAISVGKKKIPYTGSSYNPNDDGILS--ETSGNIIIDSGTTLTLLEAGFFDKFSSAV 366

Query: 361 ITLPDLQQKKEDPRNRFELCFAANANDMETFPDVTVHLDGAELILNVESTFVKIEDDGII 420
                  ++  DP+     CF + + ++   P++TVH  GA++ L+  + FVK+ +D ++
Sbjct: 367 EESVTGAKRVSDPQGLLSHCFKSGSAEI-GLPEITVHFTGADVRLSPINAFVKLSED-MV 426

Query: 421 CLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPVNCA 435
           CL+++ + + V+I GNF   ++ VGYDLE + VSF  ++C+
Sbjct: 427 CLSMVPT-TEVAIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of Cp4.1LG03g11280 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.6e-47
Identity = 141/424 (33.25%), Postives = 204/424 (48.11%), Query Frame = 0

Query: 23  GFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNYLYYNMLSKNTLDNDLSLSPT 82
           GF   L H DS  +      +T    +E  + R   RL  L         + N  S   T
Sbjct: 40  GFQIMLEHVDSGKN------LTKFQLLERAIERGSRRLQRL-------EAMLNGPSGVET 99

Query: 83  LVHEG-GEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEKGPFTKFLPSKSF 142
            V+ G GEYLM+ +IG P        DT + LIW QC  C +QC  +  P   F P  S 
Sbjct: 100 SVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPC-TQCFNQSTPI--FNPQGSS 159

Query: 143 TYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNLSSDSFSFDTTDGKHVD 202
           ++   PC S +C +L+   TC  S+ +C+Y   Y D SET G++ +++ +F +     V 
Sbjct: 160 SFSTLPCSSQLCQALSS-PTC--SNNFCQYTYGYGDGSETQGSMGTETLTFGS-----VS 219

Query: 203 VGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPFNLGSTSKMYFG 262
           +  + FGC E     G  +  G VG+ + PLSL SQL + KFSYC+ P    + S +  G
Sbjct: 220 IPNITFGCGENNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLG 279

Query: 263 SL--PVTSGGQTPLLYPNSDA---YYVKVLGISVGADDPNLDG---VFDVYDVRDGWIID 322
           SL   VT+G     L  +S     YY+ + G+SVG+    +D      +  +   G IID
Sbjct: 280 SLANSVTAGSPNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIID 339

Query: 323 SGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANANDME-TFPDVTVHLD 382
           SGTT +    +A+  +  +FI+  +L        + F+LCF   ++      P   +H D
Sbjct: 340 SGTTLTYFVNNAYQSVRQEFISQINL-PVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFD 399

Query: 383 GAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPVN 437
           G +L L  E+ F+    +G+ICLA+  S   +SI GN Q QN  V YD    VVSFA   
Sbjct: 400 GGDLELPSENYFIS-PSNGLICLAMGSSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQ 437

BLAST of Cp4.1LG03g11280 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.4e-43
Identity = 131/385 (34.03%), Postives = 191/385 (49.61%), Query Frame = 0

Query: 67  MLSKNTLDNDLSLSPTLVHEG-GEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQC 126
           M S N +    S   T V+ G GEYLM+  IG P S      DT + LIW QC  C +QC
Sbjct: 72  MRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPC-TQC 131

Query: 127 EPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNL 186
             +  P   F P  S ++   PC S  C  L   +TCN+++  C+Y   Y D S T G +
Sbjct: 132 FSQPTPI--FNPQDSSSFSTLPCESQYCQDLPS-ETCNNNE--CQYTYGYGDGSTTQGYM 191

Query: 187 SSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSY 246
           ++++F+F+T+      V  + FGC E     G  +  G +G+   PLSL SQLG+ +FSY
Sbjct: 192 ATETFTFETS-----SVPNIAFGCGEDNQGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSY 251

Query: 247 CLVPFNLGSTSKMYFGSLP--VTSGG-QTPLLYP--NSDAYYVKVLGISVGADDPNLDGV 306
           C+  +   S S +  GS    V  G   T L++   N   YY+ + GI+VG D  NL   
Sbjct: 252 CMTSYGSSSPSTLALGSAASGVPEGSPSTTLIHSSLNPTYYYITLQGITVGGD--NLGIP 311

Query: 307 FDVYDVRD----GWIIDSGTTYSSLETDAFDRLLAKF---ITLPDLQQKKEDPRNRFELC 366
              + ++D    G IIDSGTT + L  DA++ +   F   I LP +    ++  +    C
Sbjct: 312 SSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTV----DESSSGLSTC 371

Query: 367 FAANANDMET-FPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGS-PVSILGNFQ 426
           F   ++      P++++  DG  L L  ++  +    +G+ICLA+  S    +SI GN Q
Sbjct: 372 FQQPSDGSTVQVPEISMQFDGGVLNLGEQNILIS-PAEGVICLAMGSSSQLGISIFGNIQ 431

Query: 427 LQNYHVGYDLEAQVVSFAPVNCADS 437
            Q   V YDL+   VSF P  C  S
Sbjct: 432 QQETQVLYDLQNLAVSFVPTQCGAS 438

BLAST of Cp4.1LG03g11280 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.4e-35
Identity = 108/355 (30.42%), Postives = 165/355 (46.48%), Query Frame = 0

Query: 88  GEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEKGPFTKFLPSKSFTYEMEP 147
           GEY     +G P  ++    DT + + W+QC  C + C  +  P   F P+ S TY+   
Sbjct: 160 GEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPC-ADCYQQSDPV--FNPTSSSTYKSLT 219

Query: 148 CGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNLSSDSFSFDTTDGKHVDVGYLNF 207
           C +  C SL     C S+   C Y++ Y D S T G L++D+ +F    G    +  +  
Sbjct: 220 CSAPQC-SLLETSACRSNK--CLYQVSYGDGSFTVGELATDTVTF----GNSGKINNVAL 279

Query: 208 GC---SEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPFNLGSTSKMYFGSLP 267
           GC   +E   TG      G +GL    LS+ +Q+    FSYCLV  + G +S + F S+ 
Sbjct: 280 GCGHDNEGLFTGA----AGLLGLGGGVLSITNQMKATSFSYCLVDRDSGKSSSLDFNSVQ 339

Query: 268 VTSGGQTPLLYPNSDA---YYVKVLGISVGADDPNL-DGVFDV-YDVRDGWIIDSGTTYS 327
           +  G  T  L  N      YYV + G SVG +   L D +FDV      G I+D GT  +
Sbjct: 340 LGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGSGGVILDCGTAVT 399

Query: 328 SLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANANDMETFPDVTVHLDGAE-LIL 387
            L+T A++ L   F+ L    +K     + F+ C+  ++      P V  H  G + L L
Sbjct: 400 RLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLDL 459

Query: 388 NVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPVNC 434
             ++  + ++D G  C A   + S +SI+GN Q Q   + YDL   V+  +   C
Sbjct: 460 PAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

BLAST of Cp4.1LG03g11280 vs. NCBI nr
Match: XP_023528351.1 (aspartic proteinase CDR1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 892 bits (2304), Expect = 0.0
Identity = 436/436 (100.00%), Postives = 436/436 (100.00%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL
Sbjct: 10  MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 69

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD
Sbjct: 70  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 129

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE
Sbjct: 130 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 189

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 190 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 249

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV
Sbjct: 250 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 309

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND
Sbjct: 310 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 369

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD
Sbjct: 370 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 429

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPVNCADS
Sbjct: 430 LEAQVVSFAPVNCADS 445

BLAST of Cp4.1LG03g11280 vs. NCBI nr
Match: KAG6582237.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 868 bits (2243), Expect = 0.0
Identity = 424/436 (97.25%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLSLTILESTARHMVPTEVGFTARLIHRDSP+SPFYDHVMTNTA+IEATVHRSRSRL
Sbjct: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMTNTARIEATVHRSRSRL 60

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLS+ TLDNDLSLSPTLVHEGGEYLMSFNIGNP SQVMGFADTSNGLIWVQCSD
Sbjct: 61  NYLYYNMLSRKTLDNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSD 120

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNS CEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE
Sbjct: 121 CNSHCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSG LSSDSFSFDTTDGKHVDVGYLNFGCSEAPL GGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 181 TSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLIGGMQSYMGSVGLNQTPLSLISQLGI 240

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVG DDPNLDGV
Sbjct: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGTDDPNLDGV 300

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF TLP+LQQKKEDPRNRFELCFAANAND
Sbjct: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANAND 360

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD
Sbjct: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 421 LEAQVVSFAPVDCADS 436

BLAST of Cp4.1LG03g11280 vs. NCBI nr
Match: KAG7018636.1 (Aspartic proteinase CDR1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 863 bits (2230), Expect = 0.0
Identity = 422/436 (96.79%), Postives = 427/436 (97.94%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLSLTILESTARHMVPTEVGFTARLIHRDSP+SPFYDHVM NTA+IEATVHRSRSRL
Sbjct: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMMNTARIEATVHRSRSRL 60

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLS+ TLDN LSL PTLVHEGGEYLMSFNIGNP SQVMGFADTSNGLIWVQCSD
Sbjct: 61  NYLYYNMLSRKTLDNGLSLLPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSD 120

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNS CEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE
Sbjct: 121 CNSHCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSG LSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 181 TSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVG DDPNLDGV
Sbjct: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGTDDPNLDGV 300

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF TLP+LQQKKEDPRNRFELCFAANAND
Sbjct: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANAND 360

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD
Sbjct: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 421 LEAQVVSFAPVDCADS 436

BLAST of Cp4.1LG03g11280 vs. NCBI nr
Match: XP_022979793.1 (aspartic proteinase CDR1-like [Cucurbita maxima])

HSP 1 Score: 863 bits (2229), Expect = 1.73e-315
Identity = 423/436 (97.02%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLS TILESTARHMVPTEVGFTARLIHRDSP+SPFYDHVM+ TA IEAT+HRSRSRL
Sbjct: 10  MMFFLSFTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMSYTAWIEATIHRSRSRL 69

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLSK+TLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD
Sbjct: 70  NYLYYNMLSKDTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 129

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDR CKYRLVYEDNSE
Sbjct: 130 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRSCKYRLVYEDNSE 189

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 190 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 249

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL+GV
Sbjct: 250 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLEGV 309

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND
Sbjct: 310 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 369

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFP VTVH DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQN HVGYD
Sbjct: 370 METFPGVTVHFDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNCHVGYD 429

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 430 LEAQVVSFAPVDCADS 445

BLAST of Cp4.1LG03g11280 vs. NCBI nr
Match: XP_022955985.1 (aspartic proteinase CDR1-like [Cucurbita moschata])

HSP 1 Score: 862 bits (2228), Expect = 1.73e-315
Identity = 421/436 (96.56%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLSLTILESTARHMVPT+VGFTARLIHRDSP+SPFY+HVMTNTA+IEATVHRSRSRL
Sbjct: 10  MMFFLSLTILESTARHMVPTDVGFTARLIHRDSPLSPFYNHVMTNTARIEATVHRSRSRL 69

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLS+ TLDNDLSLSPTLVHEGGEYLMSFNIGNP SQVMGFADTSNGLIWVQCSD
Sbjct: 70  NYLYYNMLSRKTLDNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSD 129

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNS C+ EKGPFTK LPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE
Sbjct: 130 CNSHCDAEKGPFTKLLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 189

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSG LSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 190 TSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 249

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV
Sbjct: 250 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 309

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF TLP+LQQKKEDPRNRFELCFAANAND
Sbjct: 310 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANAND 369

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD
Sbjct: 370 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 429

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 430 LEAQVVSFAPVDCADS 445

BLAST of Cp4.1LG03g11280 vs. ExPASy TrEMBL
Match: A0A6J1IXB1 (aspartic proteinase CDR1-like OS=Cucurbita maxima OX=3661 GN=LOC111479389 PE=3 SV=1)

HSP 1 Score: 863 bits (2229), Expect = 8.36e-316
Identity = 423/436 (97.02%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLS TILESTARHMVPTEVGFTARLIHRDSP+SPFYDHVM+ TA IEAT+HRSRSRL
Sbjct: 10  MMFFLSFTILESTARHMVPTEVGFTARLIHRDSPLSPFYDHVMSYTAWIEATIHRSRSRL 69

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLSK+TLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD
Sbjct: 70  NYLYYNMLSKDTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 129

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDR CKYRLVYEDNSE
Sbjct: 130 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRSCKYRLVYEDNSE 189

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 190 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 249

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL+GV
Sbjct: 250 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLEGV 309

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND
Sbjct: 310 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 369

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFP VTVH DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQN HVGYD
Sbjct: 370 METFPGVTVHFDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNCHVGYD 429

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 430 LEAQVVSFAPVDCADS 445

BLAST of Cp4.1LG03g11280 vs. ExPASy TrEMBL
Match: A0A6J1GWK9 (aspartic proteinase CDR1-like OS=Cucurbita moschata OX=3662 GN=LOC111457819 PE=3 SV=1)

HSP 1 Score: 862 bits (2228), Expect = 8.36e-316
Identity = 421/436 (96.56%), Postives = 429/436 (98.39%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           MMFFLSLTILESTARHMVPT+VGFTARLIHRDSP+SPFY+HVMTNTA+IEATVHRSRSRL
Sbjct: 10  MMFFLSLTILESTARHMVPTDVGFTARLIHRDSPLSPFYNHVMTNTARIEATVHRSRSRL 69

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           NYLYYNMLS+ TLDNDLSLSPTLVHEGGEYLMSFNIGNP SQVMGFADTSNGLIWVQCSD
Sbjct: 70  NYLYYNMLSRKTLDNDLSLSPTLVHEGGEYLMSFNIGNPSSQVMGFADTSNGLIWVQCSD 129

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 180
           CNS C+ EKGPFTK LPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE
Sbjct: 130 CNSHCDAEKGPFTKLLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSE 189

Query: 181 TSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 240
           TSG LSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI
Sbjct: 190 TSGTLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGI 249

Query: 241 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 300
           KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV
Sbjct: 250 KKFSYCLVPFNLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGV 309

Query: 301 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANAND 360
           FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF TLP+LQQKKEDPRNRFELCFAANAND
Sbjct: 310 FDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFNTLPELQQKKEDPRNRFELCFAANAND 369

Query: 361 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 420
           METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD
Sbjct: 370 METFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYD 429

Query: 421 LEAQVVSFAPVNCADS 436
           LEAQVVSFAPV+CADS
Sbjct: 430 LEAQVVSFAPVDCADS 445

BLAST of Cp4.1LG03g11280 vs. ExPASy TrEMBL
Match: A0A1S3AZA6 (aspartic proteinase CDR1-like OS=Cucumis melo OX=3656 GN=LOC103484191 PE=3 SV=1)

HSP 1 Score: 746 bits (1926), Expect = 8.09e-270
Identity = 369/440 (83.86%), Postives = 397/440 (90.23%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           M+ +L  TILES   HMV  EVGFTARLIH DSP+SPFY+H MT TA+IEATVHRSRSRL
Sbjct: 1   MILYLLFTILESKGMHMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRL 60

Query: 61  NYLYY-NMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCS 120
           +YLYY N LS+NTLDND+SLSPTLV+EGGEYLMSFNIGNPPSQVMGF DTSNGLIWVQCS
Sbjct: 61  SYLYYINKLSENTLDNDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCS 120

Query: 121 DCNSQCEPEK-GPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDN 180
           +CNSQCEPEK GP TKFL SKSFTYEMEPCGSN CNSLTGF+TCNSSD+WCKYRLVY DN
Sbjct: 121 NCNSQCEPEKRGPTTKFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDN 180

Query: 181 SETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQL 240
             TSG LSSDSF FDT+DGK VDVG+LNFGCSEAPLTG  QSY G VGLNQTPLSLISQL
Sbjct: 181 KATSGILSSDSFGFDTSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQL 240

Query: 241 GIKKFSYCLVPFN-LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNL 300
           GIKKFSYCLVPFN LGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ 
Sbjct: 241 GIKKFSYCLVPFNSLGSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHF 300

Query: 301 DGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFA-A 360
           DGVFDVYDVRDGWIID+G TYSSLETDAFD LLAKF+ L +  Q+K DP++RFELCF  A
Sbjct: 301 DGVFDVYDVRDGWIIDTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELA 360

Query: 361 NANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYH 420
           NAND+E+FPD TVH DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYH
Sbjct: 361 NANDLESFPDATVHFDGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYH 420

Query: 421 VGYDLEAQVVSFAPVNCADS 436
           VGYDLEAQV+SFAPV+CADS
Sbjct: 421 VGYDLEAQVISFAPVDCADS 440

BLAST of Cp4.1LG03g11280 vs. ExPASy TrEMBL
Match: A0A5D3CXD4 (Aspartic proteinase CDR1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00490 PE=3 SV=1)

HSP 1 Score: 736 bits (1901), Expect = 3.08e-266
Identity = 362/425 (85.18%), Postives = 388/425 (91.29%), Query Frame = 0

Query: 16  HMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNYLYY-NMLSKNTLD 75
           HMV  EVGFTARLIH DSP+SPFY+H MT TA+IEATVHRSRSRL+YLYY N LS+NTLD
Sbjct: 2   HMVSNEVGFTARLIHHDSPLSPFYNHAMTGTARIEATVHRSRSRLSYLYYINKLSENTLD 61

Query: 76  NDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEK-GPFT 135
           ND+SLSPTLV+EGGEYLMSFNIGNPPSQVMGF DTSNGLIWVQCS+CNSQCEPEK GP T
Sbjct: 62  NDVSLSPTLVNEGGEYLMSFNIGNPPSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGPTT 121

Query: 136 KFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNLSSDSFSFD 195
           KFL SKSFTYEMEPCGSN CNSLTGF+TCNSSD+WCKYRLVY DN  TSG LSSDSF FD
Sbjct: 122 KFLSSKSFTYEMEPCGSNFCNSLTGFKTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 181

Query: 196 TTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPFN-L 255
           T+DGK VDVG+LNFGCSEAPLTG  QSY G VGLNQTPLSLISQLGIKKFSYCLVPFN L
Sbjct: 182 TSDGKLVDVGFLNFGCSEAPLTGDEQSYTGRVGLNQTPLSLISQLGIKKFSYCLVPFNSL 241

Query: 256 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGVFDVYDVRDGWII 315
           GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ DGVFDVYDVRDGWII
Sbjct: 242 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYDVRDGWII 301

Query: 316 DSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFA-ANANDMETFPDVTVHL 375
           D+G TYSSLETDAFD LLAKF+ L +  Q+K DP++RFELCF  ANAND+E+FPD TVH 
Sbjct: 302 DTGITYSSLETDAFDSLLAKFLALKNFPQRKNDPKDRFELCFELANANDLESFPDATVHF 361

Query: 376 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPV 435
           DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYHVGYDLEAQV+SFAPV
Sbjct: 362 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 421

BLAST of Cp4.1LG03g11280 vs. ExPASy TrEMBL
Match: A0A0A0L7U3 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G151520 PE=3 SV=1)

HSP 1 Score: 732 bits (1890), Expect = 1.46e-264
Identity = 361/425 (84.94%), Postives = 387/425 (91.06%), Query Frame = 0

Query: 16  HMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNYLYY-NMLSKNTLD 75
           HMV  EVGFTARLIH DSP+SPFY+H MT+TA+IEATVHRSRSRLNYLYY N LS+N LD
Sbjct: 2   HMVSNEVGFTARLIHHDSPLSPFYNHTMTDTARIEATVHRSRSRLNYLYYINKLSENALD 61

Query: 76  NDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEK-GPFT 135
           ND+SLSPTLV+EGGEYLMSFNIGNP SQVMGF DTSNGLIWVQCS+CNSQCEPEK G  T
Sbjct: 62  NDVSLSPTLVNEGGEYLMSFNIGNPSSQVMGFLDTSNGLIWVQCSNCNSQCEPEKRGLTT 121

Query: 136 KFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETSGNLSSDSFSFD 195
           KFL SKSFTYEMEPCGSN CNSLTGFQTCNSSD+WCKYRLVY DN  TSG LSSDSF FD
Sbjct: 122 KFLSSKSFTYEMEPCGSNFCNSLTGFQTCNSSDKWCKYRLVYGDNKATSGILSSDSFGFD 181

Query: 196 TTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIKKFSYCLVPFN-L 255
           T+DG  VDVG+LNFGCSEAPLTG  QSY G+VGLNQTPLSLISQLGIKKFSYCLVPFN L
Sbjct: 182 TSDGMLVDVGFLNFGCSEAPLTGDEQSYTGNVGLNQTPLSLISQLGIKKFSYCLVPFNNL 241

Query: 256 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISVGADDPNLDGVFDVYDVRDGWII 315
           GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGIS+G D+P+ DGVFDVY+VRDGWII
Sbjct: 242 GSTSKMYFGSLPVTSGGQTPLLYPNSDAYYVKVLGISIGNDEPHFDGVFDVYEVRDGWII 301

Query: 316 DSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAA-NANDMETFPDVTVHL 375
           D+G TYSSLETDAFD LLAKF+TL D  Q+K+DP+ RFELCF   NAND+E+FPDVTVH 
Sbjct: 302 DTGITYSSLETDAFDSLLAKFLTLKDFPQRKDDPKERFELCFELQNANDLESFPDVTVHF 361

Query: 376 DGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPV 435
           DGA+LILNVESTFVKIEDDGI CLALLRSGSPVSILGNFQLQNYHVGYDLEAQV+SFAPV
Sbjct: 362 DGADLILNVESTFVKIEDDGIFCLALLRSGSPVSILGNFQLQNYHVGYDLEAQVISFAPV 421

BLAST of Cp4.1LG03g11280 vs. TAIR 10
Match: AT5G33340.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-63
Identity = 159/442 (35.97%), Postives = 235/442 (53.17%), Query Frame = 0

Query: 5   LSLTILES--TARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNY 64
           LSL +L S   +      ++GFTA LIHRDSP SPFY+ + T++ ++   +HRS +R+  
Sbjct: 10  LSLCLLSSLFLSNANAKPKLGFTADLIHRDSPKSPFYNPMETSSQRLRNAIHRSVNRV-- 69

Query: 65  LYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCN 124
             ++   K   DN       L    GEYLM+ +IG PP  +M  ADT + L+W QC+ C+
Sbjct: 70  --FHFTEK---DNTPQPQIDLTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCD 129

Query: 125 SQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNSETS 184
             C  +  P   F P  S TY+   C S+ C +L    +C+++D  C Y L Y DNS T 
Sbjct: 130 -DCYTQVDPL--FDPKTSSTYKDVSCSSSQCTALENQASCSTNDNTCSYSLSYGDNSYTK 189

Query: 185 GNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLGIK- 244
           GN++ D+ +  ++D + + +  +  GC         +   G VGL   P+SLI QLG   
Sbjct: 190 GNIAVDTLTLGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSLIKQLGDSI 249

Query: 245 --KFSYCLVPF--NLGSTSKMYFGSLPVTSGG---QTPLLYPNSDA--YYVKVLGISVGA 304
             KFSYCLVP       TSK+ FG+  + SG     TPL+   S    YY+ +  ISVG+
Sbjct: 250 DGKFSYCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTPLIAKASQETFYYLTLKSISVGS 309

Query: 305 DDPNLDGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFEL 364
                 G  D        IIDSGTT + L T+ +  L     +  D  +KK+DP++   L
Sbjct: 310 KQIQYSG-SDSESSEGNIIIDSGTTLTLLPTEFYSELEDAVASSID-AEKKQDPQSGLSL 369

Query: 365 CFAANANDMETFPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQL 424
           C++A   D++  P +T+H DGA++ L+  + FV++ +D ++C A  R     SI GN   
Sbjct: 370 CYSA-TGDLKV-PVITMHFDGADVKLDSSNAFVQVSED-LVCFA-FRGSPSFSIYGNVAQ 429

Query: 425 QNYHVGYDLEAQVVSFAPVNCA 435
            N+ VGYD  ++ VSF P +CA
Sbjct: 430 MNFLVGYDTVSKTVSFKPTDCA 435

BLAST of Cp4.1LG03g11280 vs. TAIR 10
Match: AT2G35615.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 217.6 bits (553), Expect = 1.9e-56
Identity = 164/461 (35.57%), Postives = 237/461 (51.41%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRL 60
           + FFL  ++  S++ H       F+  LIHRDSP+SP Y+  +T T ++ A   RS SR 
Sbjct: 7   LCFFLFFSVTLSSSGH----PKNFSVELIHRDSPLSPIYNPQITVTDRLNAAFLRSVSR- 66

Query: 61  NYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSD 120
           +  + + LS+  L +       L+   GE+ MS  IG PP +V   ADT + L WVQC  
Sbjct: 67  SRRFNHQLSQTDLQSG------LIGADGEFFMSITIGTPPIKVFAIADTGSDLTWVQCKP 126

Query: 121 CNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQT-CNSSDRWCKYRLVYEDNS 180
           C  QC  E GP   F   KS TY+ EPC S  C +L+  +  C+ S+  CKYR  Y D S
Sbjct: 127 C-QQCYKENGPI--FDKKKSSTYKSEPCDSRNCQALSSTERGCDESNNICKYRYSYGDQS 186

Query: 181 ETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGS--VGLNQTPLSLISQ 240
            + G++++++ S D+  G  V      FGC      GG     GS  +GL    LSLISQ
Sbjct: 187 FSKGDVATETVSIDSASGSPVSFPGTVFGCGYN--NGGTFDETGSGIIGLGGGHLSLISQ 246

Query: 241 LG---IKKFSYCL----------VPFNLGSTSKMYFGSLPVTSG-GQTPLLYPNSDAYYV 300
           LG    KKFSYCL             NLG+ S     SL   SG   TPL+      YY 
Sbjct: 247 LGSSISKKFSYCLSHKSATTNGTSVINLGTNS--IPSSLSKDSGVVSTPLVDKEPLTYYY 306

Query: 301 KVL-GISVG---------ADDPNLDGVFDVYDVRDGWIIDSGTTYSSLETDAFDRLLAKF 360
             L  ISVG         + +PN DG+    +     IIDSGTT + LE   FD+  +  
Sbjct: 307 LTLEAISVGKKKIPYTGSSYNPNDDGILS--ETSGNIIIDSGTTLTLLEAGFFDKFSSAV 366

Query: 361 ITLPDLQQKKEDPRNRFELCFAANANDMETFPDVTVHLDGAELILNVESTFVKIEDDGII 420
                  ++  DP+     CF + + ++   P++TVH  GA++ L+  + FVK+ +D ++
Sbjct: 367 EESVTGAKRVSDPQGLLSHCFKSGSAEI-GLPEITVHFTGADVRLSPINAFVKLSED-MV 426

Query: 421 CLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPVNCA 435
           CL+++ + + V+I GNF   ++ VGYDLE + VSF  ++C+
Sbjct: 427 CLSMVPT-TEVAIYGNFAQMDFLVGYDLETRTVSFQHMDCS 444

BLAST of Cp4.1LG03g11280 vs. TAIR 10
Match: AT1G31450.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-51
Identity = 147/431 (34.11%), Postives = 212/431 (49.19%), Query Frame = 0

Query: 25  TARLIHRDSPVSPFYDHVMTNTAQIEATVHRSRSRLNYLYYNMLSKNTLDNDLSLSPTLV 84
           T  LIHRDSP SP Y+   T + ++ A   RS SR                   L   L+
Sbjct: 30  TVELIHRDSPHSPLYNPHHTVSDRLNAAFLRSISR----------SRRFTTKTDLQSGLI 89

Query: 85  HEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCSDCNSQCEPEKGPFTKFLPSKSFTYE 144
             GGEY MS +IG PPS+V   ADT + L WVQC  C  QC  +  P   F   KS TY+
Sbjct: 90  SNGGEYFMSISIGTPPSKVFAIADTGSDLTWVQCKPC-QQCYKQNSPL--FDKKKSSTYK 149

Query: 145 MEPCGSNVCNSLTGFQT-CNSSDRWCKYRLVYEDNSETSGNLSSDSFSFDTTDGKHVDVG 204
            E C S  C +L+  +  C+ S   CKYR  Y DNS T G++++++ S D++ G  V   
Sbjct: 150 TESCDSKTCQALSEHEEGCDESKDICKYRYSYGDNSFTKGDVATETISIDSSSGSSVSFP 209

Query: 205 YLNFGCSEAPLTGGMQSYMGS--VGLNQTPLSLISQLGI---KKFSYCL--VPFNLGSTS 264
              FGC      GG     GS  +GL   PLSL+SQLG    KKFSYCL         TS
Sbjct: 210 GTVFGCGYN--NGGTFEETGSGIIGLGGGPLSLVSQLGSSIGKKFSYCLSHTAATTNGTS 269

Query: 265 KMYFGSLPVTSGGQ-------TPLLYPNSDAYYVKVL-GISVGADD-PNLDGVFDV---Y 324
            +  G+  + S          TPL+  + + YY   L  ++VG    P   G + +    
Sbjct: 270 VINLGTNSIPSNPSKDSATLTTPLIQKDPETYYFLTLEAVTVGKTKLPYTGGGYGLNGKS 329

Query: 325 DVRDG-WIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNRFELCFAANANDMET 384
             R G  IIDSGTT + L++  +D             ++  DP+     CF +   ++  
Sbjct: 330 SKRTGNIIIDSGTTLTLLDSGFYDDFGTAVEESVTGAKRVSDPQGLLTHCFKSGDKEI-G 389

Query: 385 FPDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSILGNFQLQNYHVGYDLEA 435
            P +T+H   A++ L+  + FVK+ +D  +CL+++ + + V+I GN    ++ VGYDLE 
Sbjct: 390 LPAITMHFTNADVKLSPINAFVKLNED-TVCLSMIPT-TEVAIYGNMVQMDFLVGYDLET 442

BLAST of Cp4.1LG03g11280 vs. TAIR 10
Match: AT1G64830.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.5e-48
Identity = 141/447 (31.54%), Postives = 222/447 (49.66%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEVGFTARLIHRDSPVSPFYDHVMTNTAQIEATVHRS-RSR 60
           ++F   L++L  +  +  P + GFT  LIHRDSP SPFY+   T++ ++   + RS RS 
Sbjct: 4   LIFATLLSLLLLSNVNAYPKD-GFTIDLIHRDSPKSPFYNSAETSSQRMRNAIRRSARST 63

Query: 61  LNYLYYNMLSKNTLDNDLSLSPTLVHEGGEYLMSFNIGNPPSQVMGFADTSNGLIWVQCS 120
           L +        N   +  S    +    GEYLM+ +IG PP  ++  ADT + LIW QC+
Sbjct: 64  LQF-------SNDDASPNSPQSFITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCN 123

Query: 121 DCNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKYRLVYEDNS 180
            C   C  +  P   F P +S TY    C S+ C +L    +C++ +  C Y + Y DNS
Sbjct: 124 PC-EDCYQQTSPL--FDPKESSTYRKVSCSSSQCRALED-ASCSTDENTCSYTITYGDNS 183

Query: 181 ETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTPLSLISQLG 240
            T G+++ D+ +  ++  + V +  +  GC          +  G +GL     SL+SQL 
Sbjct: 184 YTKGDVAVDTVTMGSSGRRPVSLRNMIIGCGHENTGTFDPAGSGIIGLGGGSTSLVSQLR 243

Query: 241 IK---KFSYCLVPF--NLGSTSKMYFGSLPVTSGG---QTPLLYPNSDAYY-VKVLGISV 300
                KFSYCLVPF    G TSK+ FG+  + SG     T ++  +   YY + +  ISV
Sbjct: 244 KSINGKFSYCLVPFTSETGLTSKINFGTNGIVSGDGVVSTSMVKKDPATYYFLNLEAISV 303

Query: 301 GADDPNLDGVFDVYDVRDG-WIIDSGTTYSSLETDAFDRLLAKFITLPDLQQKKEDPRNR 360
           G+    +     ++   +G  +IDSGTT + L ++ F   L   +      ++ +DP   
Sbjct: 304 GS--KKIQFTSTIFGTGEGNIVIDSGTTLTLLPSN-FYYELESVVASTIKAERVQDPDGI 363

Query: 361 FELCFAANANDMETF--PDVTVHLDGAELILNVESTFVKIEDDGIICLALLRSGSPVSIL 420
             LC+     D  +F  PD+TVH  G ++ L   +TFV + +D + C A   +   ++I 
Sbjct: 364 LSLCY----RDSSSFKVPDITVHFKGGDVKLGNLNTFVAVSED-VSCFA-FAANEQLTIF 423

Query: 421 GNFQLQNYHVGYDLEAQVVSFAPVNCA 435
           GN    N+ VGYD  +  VSF   +C+
Sbjct: 424 GNLAQMNFLVGYDTVSGTVSFKKTDCS 429

BLAST of Cp4.1LG03g11280 vs. TAIR 10
Match: AT2G03200.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 183.7 bits (465), Expect = 3.1e-46
Identity = 150/460 (32.61%), Postives = 222/460 (48.26%), Query Frame = 0

Query: 1   MMFFLSLTILESTARHMVPTEV-------GFTARLIHRDSPVSPFYDHVMTNTAQIEATV 60
           ++ F  L  + S+ R ++   +       GF   L H DS  +      +T   +I+  +
Sbjct: 15  LILFSCLISVSSSRRSLIDRTLPKNLPRSGFRLSLRHVDSGKN------LTKIQKIQRGI 74

Query: 61  HRSRSRLNYL-YYNMLSKNTLDNDLSLSPTLVHEG-GEYLMSFNIGNPPSQVMGFADTSN 120
           +R   RLN L    +L+  +  +D +      H G GE+LM  +IGNP  +     DT +
Sbjct: 75  NRGFHRLNRLGAVAVLAVASKPDDTNNIKAPTHGGSGEFLMELSIGNPAVKYSAIVDTGS 134

Query: 121 GLIWVQCSDCNSQCEPEKGPFTKFLPSKSFTYEMEPCGSNVCNSLTGFQTCNSSDRWCKY 180
            LIW QC  C ++C  +  P   F P KS +Y    C S +CN+L     CN     C+Y
Sbjct: 135 DLIWTQCKPC-TECFDQPTPI--FDPEKSSSYSKVGCSSGLCNALPR-SNCNEDKDACEY 194

Query: 181 RLVYEDNSETSGNLSSDSFSFDTTDGKHVDVGYLNFGCSEAPLTGGMQSYMGSVGLNQTP 240
              Y D S T G L++++F+F+  +     +  + FGC       G     G VGL + P
Sbjct: 195 LYTYGDYSSTRGLLATETFTFEDEN----SISGIGFGCGVENEGDGFSQGSGLVGLGRGP 254

Query: 241 LSLISQLGIKKFSYCLVPF-NLGSTSKMYFGSLP---VTSGG--------QTPLLYPNSD 300
           LSLISQL   KFSYCL    +  ++S ++ GSL    V   G        +T  L  N D
Sbjct: 255 LSLISQLKETKFSYCLTSIEDSEASSSLFIGSLASGIVNKTGASLDGEVTKTMSLLRNPD 314

Query: 301 A---YYVKVLGISVGADDPNLD-GVFDV-YDVRDGWIIDSGTTYSSLETDAFDRLLAKFI 360
               YY+++ GI+VGA   +++   F++  D   G IIDSGTT + LE  AF  L  +F 
Sbjct: 315 QPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDSGTTITYLEETAFKVLKEEFT 374

Query: 361 TLPDLQQKKEDPRNRFELCF-AANANDMETFPDVTVHLDGAELILNVESTFVKIEDDGII 420
           +   L    +      +LCF   +A      P +  H  GA+L L  E+  V     G++
Sbjct: 375 SRMSL-PVDDSGSTGLDLCFKLPDAAKNIAVPKMIFHFKGADLELPGENYMVADSSTGVL 434

Query: 421 CLALLRSGSPVSILGNFQLQNYHVGYDLEAQVVSFAPVNC 434
           CLA + S + +SI GN Q QN++V +DLE + VSF P  C
Sbjct: 435 CLA-MGSSNGMSIFGNVQQQNFNVLHDLEKETVSFVPTEC 458