Cp4.1LG03g08830 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g08830
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	O-methyltransferase family protein
Location	Cp4.1LG03: 5993312 .. 5994454 (-)
RNA-Seq Expression	Cp4.1LG03g08830
Synteny	Cp4.1LG03g08830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGAGAAGAACACAGATGAGTTGATTGAAGCTCAAGCCCATATTTGGAACCATACATTCAAATACATCAATTCCATGTCTTTGAAGTGTGTTATTGAATTGGGCGTACCAGATATCATCCATAACCATGGCCAACCCATGACCCTATCAGAATTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCCCATTGCCTTGGCGTTCTTATGCGCCTCCTCCATCATTCTGGCTTCTTTTCTCAAACTCAACACCAGAATCATCAGCATCAAGAAGTAGTGAAGTATGGTCTTACACCCTCCTCTCGATTTCTCCTCAGCCCCAATATGACGACTTCAACGACGACGACATTACAAGCCTTACCCTTCGTGTTTCTAGCCCTCCATCAAGCCATGATGGCTCCATGGGAAACCATGAGCAGCTGGCTGCGCACTGCAGATGGGTCTGCCTTTGAAACGGTGCATGGGAAGTCAATATGGAATTACATTGCTGATGAGCCTGATCTTAGAAATTTGTTCCATCAATCCGTGGCTTATGATTCTCACCTGATTGGGAGGATCGTGACGAGGCCGGAGTGCCGAGACGTGTTCGAGGGGGTGAAGTCACTGGTCGATGTTGCCGGCGATCGAGGCATTATAGCCAATGCCATAGCGGAAGCATTCCCACACATCACTTGCACTGTGTTGGATCTCCCGCAACAAACCACAAAAGGGTTGAACTTAGCAATACAAATTCCACCAACAGATGCAGTCTTACTCAAGGTATGAAGGTTGTTTCCCTTTCGATTTTAAACCTTAAACATTCCGAAAAAGTACTTAGTTTGGGATTTTGGTGATCATCAGTCAGTTCTACACCAATGCAACGATGAAGAAAGCATACAAATACTGAAGAAATGTAAAGATGCAATTAGTAAGGGCAAAGGTGGGAAAGTGATTATCATAGAGGCAGTGCTGGAAAGGCAGATGGAAGATGAGGAGTCAACTGAAACTGAGCTTTGTGGTAATGCGTTGATGATGGCCACTTTCAACAATACAAAGAGAAATGAGAGAGAATGGAAGGATCTGTTCATGGCAGCTGGTTTTAGTAACTATAAGATAACTACCTTCTTGGGTTTAAGGTCTCTTATTGAGGTCTTCCCTTGA

mRNA sequence

ATGGGAGAGAAGAACACAGATGAGTTGATTGAAGCTCAAGCCCATATTTGGAACCATACATTCAAATACATCAATTCCATGTCTTTGAAGTGTGTTATTGAATTGGGCGTACCAGATATCATCCATAACCATGGCCAACCCATGACCCTATCAGAATTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCCCATTGCCTTGGCGTTCTTATGCGCCTCCTCCATCATTCTGGCTTCTTTTCTCAAACTCAACACCAGAATCATCAGCATCAAGAAGTAGTGAAGTATGGTCTTACACCCTCCTCTCGATTTCTCCTCAGCCCCAATATGACGACTTCAACGACGACGACATTACAAGCCTTACCCTTCGTGTTTCTAGCCCTCCATCAAGCCATGATGGCTCCATGGGAAACCATGAGCAGCTGGCTGCGCACTGCAGATGGGTCTGCCTTTGAAACGGTGCATGGGAAGTCAATATGGAATTACATTGCTGATGAGCCTGATCTTAGAAATTTGTTCCATCAATCCGTGGCTTATGATTCTCACCTGATTGGGAGGATCGTGACGAGGCCGGAGTGCCGAGACGTGTTCGAGGGGGTGAAGTCACTGGTCGATGTTGCCGGCGATCGAGGCATTATAGCCAATGCCATAGCGGAAGCATTCCCACACATCACTTGCACTGTGTTGGATCTCCCGCAACAAACCACAAAAGGGTTGAACTTAGCAATACAAATTCCACCAACAGATGCAGTCTTACTCAAGTCAGTTCTACACCAATGCAACGATGAAGAAAGCATACAAATACTGAAGAAATGTAAAGATGCAATTAGTAAGGGCAAAGGTGGGAAAGTGATTATCATAGAGGCAGTGCTGGAAAGGCAGATGGAAGATGAGGAGTCAACTGAAACTGAGCTTTGTGGTAATGCGTTGATGATGGCCACTTTCAACAATACAAAGAGAAATGAGAGAGAATGGAAGGATCTGTTCATGGCAGCTGGTTTTAGTAACTATAAGATAACTACCTTCTTGGGTTTAAGGTCTCTTATTGAGGTCTTCCCTTGA

Coding sequence (CDS)

ATGGGAGAGAAGAACACAGATGAGTTGATTGAAGCTCAAGCCCATATTTGGAACCATACATTCAAATACATCAATTCCATGTCTTTGAAGTGTGTTATTGAATTGGGCGTACCAGATATCATCCATAACCATGGCCAACCCATGACCCTATCAGAATTAGTGGAAGCGCTCCATATTCAACCCTCCAAAGCCCATTGCCTTGGCGTTCTTATGCGCCTCCTCCATCATTCTGGCTTCTTTTCTCAAACTCAACACCAGAATCATCAGCATCAAGAAGTAGTGAAGTATGGTCTTACACCCTCCTCTCGATTTCTCCTCAGCCCCAATATGACGACTTCAACGACGACGACATTACAAGCCTTACCCTTCGTGTTTCTAGCCCTCCATCAAGCCATGATGGCTCCATGGGAAACCATGAGCAGCTGGCTGCGCACTGCAGATGGGTCTGCCTTTGAAACGGTGCATGGGAAGTCAATATGGAATTACATTGCTGATGAGCCTGATCTTAGAAATTTGTTCCATCAATCCGTGGCTTATGATTCTCACCTGATTGGGAGGATCGTGACGAGGCCGGAGTGCCGAGACGTGTTCGAGGGGGTGAAGTCACTGGTCGATGTTGCCGGCGATCGAGGCATTATAGCCAATGCCATAGCGGAAGCATTCCCACACATCACTTGCACTGTGTTGGATCTCCCGCAACAAACCACAAAAGGGTTGAACTTAGCAATACAAATTCCACCAACAGATGCAGTCTTACTCAAGTCAGTTCTACACCAATGCAACGATGAAGAAAGCATACAAATACTGAAGAAATGTAAAGATGCAATTAGTAAGGGCAAAGGTGGGAAAGTGATTATCATAGAGGCAGTGCTGGAAAGGCAGATGGAAGATGAGGAGTCAACTGAAACTGAGCTTTGTGGTAATGCGTTGATGATGGCCACTTTCAACAATACAAAGAGAAATGAGAGAGAATGGAAGGATCTGTTCATGGCAGCTGGTTTTAGTAACTATAAGATAACTACCTTCTTGGGTTTAAGGTCTCTTATTGAGGTCTTCCCTTGA

Protein sequence

MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP

Homology

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BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 5.8e-88
Identity = 175/368 (47.55%), Postives = 239/368 (64.95%), Query Frame = 0


Query: 1   MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ 60
           + E+N+ E++ AQAHIWN  F +INSMSLKC I+LG+PDII+NHG+PMT+S+L  AL I 
Sbjct: 18  LNEENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPIN 77

Query: 61  PSKAHCLGVLMRLLHHSGFFSQTQHQNHQH--QEVVKYGLTPSSRFLLSPNMTTSTTTTL 120
             K+ C+  LMR+L HSGFF+  + +  +    E   Y +T +S+ LL  N  + T    
Sbjct: 78  RKKSPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVT---- 137

Query: 121 QALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVA 180
              PF+   L   M  PW+ +S+W +  D + F+T +G + W+Y + EP L   F+ ++A
Sbjct: 138 ---PFLLAMLDPVMTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMA 197

Query: 181 YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTK- 240
            D+ L+  +V   +C+ VFEGV+SLVDV G  G +A++IA AFPHI CTV DLP      
Sbjct: 198 SDARLVTSVVIE-KCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADL 257

Query: 241 ---------GLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKG--KGGKVII 300
                    G ++ + +P T+ VLLK +LH  NDEES++ILKKCK+AISK   KGGKVII
Sbjct: 258 QGGNNLNFVGGDMFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVII 317

Query: 301 IEAVLERQM-EDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGL 354
           I+  +E +  ED+ES ET+L  + LMMA     +RNE+EW  LF  AGFSNYKIT  LGL
Sbjct: 318 IDMKVENEKDEDDESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGL 377

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 307.0 bits (785), Expect = 2.8e-82
Identity = 165/357 (46.22%), Postives = 228/357 (63.87%), Query Frame = 0


Query: 8   ELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQPSKAHCL 67
           EL+ AQAH+WNH F +I SMSLKC I+LG+PDIIHNHG+PMTL ELV  L + P ++ C+
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 68  GVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFVFLA 127
             LMR+L HSGF +  + Q  + +E   Y LT +SR LL  +       +L   P V   
Sbjct: 71  YRLMRILVHSGFLAAQRVQQGKEEE--GYVLTDASRLLLMDD-------SLSIRPLVLAM 130

Query: 128 LHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLIGRI 187
           L   +  PW  +S+W +  D + F T + +S W+Y   EP L N F++++A D+ L+  +
Sbjct: 131 LDPILTKPWHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSV 190

Query: 188 VTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ-----QTTKGLN-- 247
           + + E + VF G+ SLVDV G  G +A AIA AFPH+ CTVLDL       Q +K LN  
Sbjct: 191 LLK-EGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSKNLNYF 250

Query: 248 ---LAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAI-SKGKGGKVIIIEAVLERQME 307
              +   IPP DA+LLK +LH  ++EE ++ILK+C++AI SK  GGKVIII+ ++ +   
Sbjct: 251 AGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQG 310

Query: 308 DEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 354
           D +STET+L  +  MM      +R+E EW+ LF+ AGFS+YKIT  LGLRSLIEV+P
Sbjct: 311 DYKSTETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.2e-78
Identity = 162/369 (43.90%), Postives = 223/369 (60.43%), Query Frame = 0


Query: 2   GEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQP 61
           G  +  EL+EAQAH+WNH + YINSMSLKC I+LG+PD IH HG P+TLS+L +AL+I  
Sbjct: 6   GISSKQELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINK 65

Query: 62  SKAHCLGVLMRLLHHSGFFSQTQ-----HQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTT 121
           +K+H L  LMR+L HSGFF + +         + +E   Y LTP+SR LL        + 
Sbjct: 66  AKSHGLFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLL-------RSE 125

Query: 122 TLQALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQS 181
            L   PF            W  +S W R    +AF+T +G +   Y   +  L  LF+++
Sbjct: 126 PLSVAPFALAMSDPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEA 185

Query: 182 VAYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQT- 241
           +A D+  +  I+T  ECR++F+G++S+VDV G  G  A  IA AFP + CTVLDLP    
Sbjct: 186 MACDAGFVNSILT-TECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVG 245

Query: 242 -TKGL-NLAIQ-------IPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGG--KV 301
             KG  NL+         IP  DA+ +K +LH  NDEE ++ILKKCK+AIS+      K+
Sbjct: 246 GLKGSENLSFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKI 305

Query: 302 IIIEAVLERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLG 354
           I++E V+E + E  E+TET+L  +  M+A     +R+E+EW  LF  AGF+NYKIT  LG
Sbjct: 306 ILVEIVMEDEKETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLG 365

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 6.2e-74
Identity = 166/362 (45.86%), Postives = 230/362 (63.54%), Query Frame = 0


Query: 7   DELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQP--MTLSELVEALHIQPSKA 66
           +EL+ AQAHI+NH F +I+SM+LKC +EL +P+I+HNH QP  +TLSELV+AL I  +K+
Sbjct: 22  EELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNH-QPKAVTLSELVQALQIPQAKS 81

Query: 67  HCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFV 126
            CL  L+R+L HSGFF+ T+ Q+   +E   Y  T SS+ LL  N   S +  L  L   
Sbjct: 82  ACLYRLLRILVHSGFFAITKIQSEGDEE--GYLPTLSSKLLLK-NHPMSMSPCLLGL--- 141

Query: 127 FLALHQAMMAPWETMSSWL-RTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHL 186
              ++  M+AP    S W  R+ D + FE  HG S+W Y  + P +  +F++++  ++ L
Sbjct: 142 ---VNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCETRL 201

Query: 187 IGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ--QTTKGLN- 246
              +V + EC+   EG+ SLVDV G  G +  AIAEAFP++ CTVLDLPQ     KG N 
Sbjct: 202 AMSVVLK-ECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGSNN 261

Query: 247 -------LAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAI-SKGKGGKVIIIEAVL- 306
                  +   IPP D V LK +LH  NDEE I+ILK+CK+AI SK +GGK+III+ V+ 
Sbjct: 262 LEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDMVVN 321

Query: 307 ERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEV 354
           +      ESTET+L  +  +MA    T+R E EWK LF+AAGF++Y I+  LGL+S+IEV
Sbjct: 322 DHNKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLKSIIEV 372

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot
Match: Q84KK4 (Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 2.4e-73
Identity = 155/368 (42.12%), Postives = 223/368 (60.60%), Query Frame = 0


Query: 2   GEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQP 61
           G ++T EL +AQ H++ H + +++SM+LK  +ELG+ D+IH+HG+P+TL EL  AL+++P
Sbjct: 8   GSEDT-ELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHGKPITLPELATALNLRP 67

Query: 62  SKAHCLGVLMRLLHHSGFFSQTQ-HQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA 121
           SK   L   +RLL H+GFF++T   +    +E   YGLTP S+ L+  N T         
Sbjct: 68  SKIGVLHRFLRLLTHNGFFAKTTVSRGEGAEEETAYGLTPPSKLLVKSNSTC-------L 127

Query: 122 LPFVFLALHQAMMAPWETMSSWL--RTADGSAFETVHGKSIWNYI--ADEPDLRNLFHQS 181
            P V  ALH + +  W +   W      + + FE+  G+S W ++    E D  ++F ++
Sbjct: 128 APIVKGALHPSSLDMWRSSKKWFLEDNEELTLFESATGESFWEFLNKETESDTLSMFQEA 187

Query: 182 VAYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTT 241
           +A DSH+    +   EC+ VFEG+ SLVDVAG RG +   I EAFPH+ CTV D PQ   
Sbjct: 188 MAADSHMFK--LALKECKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVA 247

Query: 242 K----------GLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAIS-KGKGGKVI 301
                      G ++   +PP DAVLLK VLH  NDE S++ILK CK+AIS +GK GKVI
Sbjct: 248 NLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVI 307

Query: 302 IIEAVLERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGL 354
           II+  ++   +D E TE +L  + +M+  FN  +R ++EW+ L   AGFS+YKIT   G 
Sbjct: 308 IIDISIDETSDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFSSYKITPICGF 365

[Close]

BLAST of Cp4.1LG03g08830 vs. NCBI nr
Match: XP_023520750.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo] >XP_023528579.1 probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712 bits (1838), Expect = 5.85e-259
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ 60
           MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ
Sbjct: 1   MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ 60

Query: 61  PSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA 120
           PSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA
Sbjct: 61  PSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA 120

Query: 121 LPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYD 180
           LPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYD
Sbjct: 121 LPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYD 180

Query: 181 SHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGLN 240
           SHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGLN
Sbjct: 181 SHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGLN 240

Query: 241 LAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEES 300
           LAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEES
Sbjct: 241 LAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEES 300

Query: 301 TETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           TETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP
Sbjct: 301 TETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353

[Close]

BLAST of Cp4.1LG03g08830 vs. NCBI nr
Match: KAG6581634.1 (putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1788), Expect = 2.55e-251
Identity = 345/354 (97.46%), Postives = 347/354 (98.02%), Query Frame = 0


Query: 1   MGEK-NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHI 60
           MGEK NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPM+LSELVEALHI
Sbjct: 1   MGEKENTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMSLSELVEALHI 60

Query: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQ 120
           QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPN TTSTTTTLQ
Sbjct: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNTTTSTTTTLQ 120

Query: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAY 180
           ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAY
Sbjct: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAY 180

Query: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240
           DSHLIGRIVTRPECRDVFEGVKSLVDVA DRGIIANAIAEAFPHITCTVLDLPQQTTKGL
Sbjct: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVASDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240

Query: 241 NLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEE 300
           NLAIQIPPTDAVLLKSVLHQCNDEE IQILKKCKDAIS  KGGKV+IIEAVLE QMEDEE
Sbjct: 241 NLAIQIPPTDAVLLKSVLHQCNDEEGIQILKKCKDAISSAKGGKVVIIEAVLETQMEDEE 300

Query: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP
Sbjct: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 354

[Close]

BLAST of Cp4.1LG03g08830 vs. NCBI nr
Match: XP_022935256.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 686 bits (1771), Expect = 9.94e-249
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0


Query: 1   MGEK-NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHI 60
           MGEK NTDELIEAQA IWNHTFKYINSMSLKCVIELGVPDIIHNHGQP++LSELVEALHI
Sbjct: 1   MGEKENTDELIEAQARIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPISLSELVEALHI 60

Query: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQ 120
           QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPN TTSTTTTLQ
Sbjct: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNTTTSTTTTLQ 120

Query: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAY 180
           ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNY+ADEPDLRNLFHQSVAY
Sbjct: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYVADEPDLRNLFHQSVAY 180

Query: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240
           DSHLIGRIVTRPECRDVFEGVKSLVDVA DRGIIANAIAEAFPHITCTVLDLPQQTTKGL
Sbjct: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVASDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240

Query: 241 NLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEE 300
           NLAIQIPPTDAVLLKSVLHQCNDEE IQILKKCKDAIS  KGGKV+IIEAVLE QMEDEE
Sbjct: 241 NLAIQIPPTDAVLLKSVLHQCNDEEGIQILKKCKDAISSAKGGKVVIIEAVLETQMEDEE 300

Query: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKI+TFLGLRSLIEVFP
Sbjct: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKISTFLGLRSLIEVFP 354

[Close]

BLAST of Cp4.1LG03g08830 vs. NCBI nr
Match: XP_022982782.1 (probable O-methyltransferase 3 [Cucurbita maxima])

HSP 1 Score: 671 bits (1730), Expect = 1.83e-242
Identity = 338/355 (95.21%), Postives = 341/355 (96.06%), Query Frame = 0


Query: 1   MGEK-NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHI 60
           MGEK N DELIEAQAHIWNH FKYINSMSLKCVIELGVPD IHNHGQPM+LSELVEALHI
Sbjct: 1   MGEKENKDELIEAQAHIWNHAFKYINSMSLKCVIELGVPDTIHNHGQPMSLSELVEALHI 60

Query: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTT-L 120
           QPSKAHCLGVLMRLLHHSGFFSQTQ QNHQ QEVVKYGLTPSSRFLL PN TTSTTTT L
Sbjct: 61  QPSKAHCLGVLMRLLHHSGFFSQTQDQNHQPQEVVKYGLTPSSRFLLRPNTTTSTTTTTL 120

Query: 121 QALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVA 180
           QALPFVFLALHQAMMAPWETMSSWLRTADGSAFET+HGKSIWNYIADEPDLRNLFHQSVA
Sbjct: 121 QALPFVFLALHQAMMAPWETMSSWLRTADGSAFETLHGKSIWNYIADEPDLRNLFHQSVA 180

Query: 181 YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG 240
           YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG
Sbjct: 181 YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG 240

Query: 241 LNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDE 300
           LNLAIQIPPTDAVLLKSVLHQCNDEE IQILKKCKDAIS  KGGKV+IIEAVLE QMED 
Sbjct: 241 LNLAIQIPPTDAVLLKSVLHQCNDEEGIQILKKCKDAISSVKGGKVVIIEAVLETQMEDA 300

Query: 301 ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP
Sbjct: 301 ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 355

[Close]

BLAST of Cp4.1LG03g08830 vs. NCBI nr
Match: XP_038905418.1 (probable O-methyltransferase 3 [Benincasa hispida])

HSP 1 Score: 484 bits (1247), Expect = 6.47e-169
Identity = 253/360 (70.28%), Postives = 294/360 (81.67%), Query Frame = 0


Query: 7   DEL-IEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQPSKAH 66
           DEL +EAQAHIWNHT KYINSMSLKCV+ELG+PDIIHNHGQPM+LS+LVEALHI PSK+ 
Sbjct: 3   DELFLEAQAHIWNHTLKYINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPSKSQ 62

Query: 67  CLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFVF 126
            L  LMRLL HSGFFSQTQ Q      V KYGLTPSSR LL  N  T+TTTT++ LPF+ 
Sbjct: 63  PLARLMRLLVHSGFFSQTQQQL-----VTKYGLTPSSRLLLRRN--TTTTTTIETLPFLV 122

Query: 127 LALHQAMMAPWETMSSWLRTAD-GSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLI 186
           L LHQAMMAPWE+MSSWLR+ + GSAFE V+GKS+W YI DEPD  +LF+Q++ YDS LI
Sbjct: 123 LILHQAMMAPWESMSSWLRSEEYGSAFEMVNGKSVWKYIGDEPDFGSLFYQTLVYDSQLI 182

Query: 187 GRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGLNLA-- 246
           G++V RPECR+VF+GVKS+VDV G  GI+A AI EAFPHI C VLDLP QTTK LN+   
Sbjct: 183 GKLVKRPECREVFDGVKSMVDVGGGEGIVAKAIEEAFPHINCIVLDLPYQTTKDLNVIGG 242

Query: 247 ------IQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKG-KGGKVIIIEAVLER-- 306
                 IQIPPTDAVLL SVLH+ NDEE I+ILK CKDAI+   +GGKVIIIEAVLE+  
Sbjct: 243 GNLFEPIQIPPTDAVLLVSVLHKYNDEEIIRILKNCKDAITNSNEGGKVIIIEAVLEKEK 302

Query: 307 QMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           QME +ES ETELCG+ LMMATFN TKRNE+EWK LF+AAGFS++KIT+FLGLRSLI+++P
Sbjct: 303 QMEHKESIETELCGDVLMMATFNTTKRNEKEWKTLFLAAGFSHHKITSFLGLRSLIQLYP 355

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL
Match: A0A6J1F418 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442193 PE=3 SV=1)

HSP 1 Score: 686 bits (1771), Expect = 4.81e-249
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0


Query: 1   MGEK-NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHI 60
           MGEK NTDELIEAQA IWNHTFKYINSMSLKCVIELGVPDIIHNHGQP++LSELVEALHI
Sbjct: 1   MGEKENTDELIEAQARIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPISLSELVEALHI 60

Query: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQ 120
           QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPN TTSTTTTLQ
Sbjct: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNTTTSTTTTLQ 120

Query: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAY 180
           ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNY+ADEPDLRNLFHQSVAY
Sbjct: 121 ALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYVADEPDLRNLFHQSVAY 180

Query: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240
           DSHLIGRIVTRPECRDVFEGVKSLVDVA DRGIIANAIAEAFPHITCTVLDLPQQTTKGL
Sbjct: 181 DSHLIGRIVTRPECRDVFEGVKSLVDVASDRGIIANAIAEAFPHITCTVLDLPQQTTKGL 240

Query: 241 NLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEE 300
           NLAIQIPPTDAVLLKSVLHQCNDEE IQILKKCKDAIS  KGGKV+IIEAVLE QMEDEE
Sbjct: 241 NLAIQIPPTDAVLLKSVLHQCNDEEGIQILKKCKDAISSAKGGKVVIIEAVLETQMEDEE 300

Query: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKI+TFLGLRSLIEVFP
Sbjct: 301 STETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKISTFLGLRSLIEVFP 354

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL
Match: A0A6J1IXH8 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481540 PE=3 SV=1)

HSP 1 Score: 671 bits (1730), Expect = 8.86e-243
Identity = 338/355 (95.21%), Postives = 341/355 (96.06%), Query Frame = 0


Query: 1   MGEK-NTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHI 60
           MGEK N DELIEAQAHIWNH FKYINSMSLKCVIELGVPD IHNHGQPM+LSELVEALHI
Sbjct: 1   MGEKENKDELIEAQAHIWNHAFKYINSMSLKCVIELGVPDTIHNHGQPMSLSELVEALHI 60

Query: 61  QPSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTT-L 120
           QPSKAHCLGVLMRLLHHSGFFSQTQ QNHQ QEVVKYGLTPSSRFLL PN TTSTTTT L
Sbjct: 61  QPSKAHCLGVLMRLLHHSGFFSQTQDQNHQPQEVVKYGLTPSSRFLLRPNTTTSTTTTTL 120

Query: 121 QALPFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVA 180
           QALPFVFLALHQAMMAPWETMSSWLRTADGSAFET+HGKSIWNYIADEPDLRNLFHQSVA
Sbjct: 121 QALPFVFLALHQAMMAPWETMSSWLRTADGSAFETLHGKSIWNYIADEPDLRNLFHQSVA 180

Query: 181 YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG 240
           YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG
Sbjct: 181 YDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKG 240

Query: 241 LNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDE 300
           LNLAIQIPPTDAVLLKSVLHQCNDEE IQILKKCKDAIS  KGGKV+IIEAVLE QMED 
Sbjct: 241 LNLAIQIPPTDAVLLKSVLHQCNDEEGIQILKKCKDAISSVKGGKVVIIEAVLETQMEDA 300

Query: 301 ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 353
           ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP
Sbjct: 301 ESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEVFP 355

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL
Match: A0A6J1DFY3 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE=3 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 2.06e-156
Identity = 242/369 (65.58%), Postives = 280/369 (75.88%), Query Frame = 0


Query: 1   MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ 60
           MGE    EL+EAQ HIWNH FKYINSMSLKCV+ELG+PDI+H HGQPM+LS+LVEALHIQ
Sbjct: 1   MGE--ISELLEAQVHIWNHAFKYINSMSLKCVVELGIPDILHTHGQPMSLSDLVEALHIQ 60

Query: 61  PSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA 120
           PSKA CLG LMRLL HSGFF++T  +N + +   KY +TPSSR LL  N       TL+ 
Sbjct: 61  PSKAQCLGRLMRLLVHSGFFARTHSENDEQE--TKYAVTPSSRLLLRHN------DTLET 120

Query: 121 LPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYIADEPDLRNLFHQSV 180
           LPF+FL LH AMMA WETMS WL + D    SAFE  +GKSIWNYIA+EP+  NLFH+++
Sbjct: 121 LPFLFLILHHAMMASWETMSCWLCSVDENYSSAFEMANGKSIWNYIAEEPEFGNLFHRTI 180

Query: 181 AYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ---- 240
           AYDS LIGRIVT  ECR+VFEG+KS+VDV G  GI+A AI EAFPHIT  V DLPQ    
Sbjct: 181 AYDSQLIGRIVT-TECREVFEGLKSIVDVGGGTGIMAKAIVEAFPHITYIVFDLPQVVAN 240

Query: 241 --QTTKGLNLA------IQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAI-SKGKGGKV 300
             QTTK L+         +IPPT+AVLLKSVL+  NDEESIQILK CKDAI S  +GGKV
Sbjct: 241 HQQTTKNLSFIGGDMFEAKIPPTNAVLLKSVLYNWNDEESIQILKNCKDAILSNAEGGKV 300

Query: 301 IIIEAVLERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLG 353
           IIIE  LE Q+ED+ES ET+LCG+ LMM  F   KRNEREWK+LF+AAGFS+YKI   LG
Sbjct: 301 IIIEVALEYQVEDKESIETQLCGDVLMMTNFAIRKRNEREWKNLFLAAGFSDYKIIPMLG 358

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL
Match: A0A6J1E096 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE=3 SV=1)

HSP 1 Score: 429 bits (1103), Expect = 2.51e-147
Identity = 227/369 (61.52%), Postives = 276/369 (74.80%), Query Frame = 0


Query: 1   MGEKNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQ 60
           MGE    EL+ AQAH+WNHTFKYINSMSLKCV+ELG+PDIIH+HGQPM+LS LV+ALHIQ
Sbjct: 1   MGEST--ELLRAQAHVWNHTFKYINSMSLKCVVELGIPDIIHSHGQPMSLSNLVDALHIQ 60

Query: 61  PSKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQA 120
           PSKA CL  LMRLL H+GFF QT   N Q +    Y LTPSSR LL  N      T  + 
Sbjct: 61  PSKAQCLNRLMRLLVHAGFFIQTHGDNEQEE----YALTPSSRLLLCRN------TAFEN 120

Query: 121 LPFVFLALHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYIADEPDLRNLFHQSV 180
           +PFVFL L++AMMAP ETMSSWL + D    SAFE  +GKSIW+Y++ E D   LFHQ++
Sbjct: 121 IPFVFLILNRAMMAPLETMSSWLCSVDEHSNSAFEMANGKSIWDYVSQEHDFGKLFHQTM 180

Query: 181 AYDSHLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ---- 240
            +DS LIG++VTR ECR+VFEG+KSLVDV G  G +A AIAEAFPHITCTV DLPQ    
Sbjct: 181 VHDSQLIGKVVTR-ECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAAN 240

Query: 241 --QTTKGLNLA------IQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAI-SKGKGGKV 300
             QTTK L+         +IPP +AVLLK +LH  NDEESI+ILK CKDAI S+ + GKV
Sbjct: 241 SQQTTKNLSFIGGDMFEAKIPPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKV 300

Query: 301 IIIEAVLERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLG 353
           I+I+ V+E Q+ED+ES E +LC + L+M ++N  +RNEREWK+LF+ AGFS+YKI + LG
Sbjct: 301 IVIDMVVENQIEDKESVEAQLCCDLLVMTSYNGRERNEREWKNLFLTAGFSDYKIISMLG 356

[Close]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL
Match: A0A6J1DHJ0 (trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111020124 PE=3 SV=1)

HSP 1 Score: 402 bits (1034), Expect = 7.78e-137
Identity = 212/362 (58.56%), Postives = 265/362 (73.20%), Query Frame = 0


Query: 8   ELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNHGQPMTLSELVEALHIQPSKAHCL 67
           EL++AQAH+WNH ++Y++SMSLKC +ELG+PDIIH+HG+PM+LS LV ALHIQP KAHCL
Sbjct: 6   ELLKAQAHVWNHAYRYMDSMSLKCAVELGIPDIIHSHGEPMSLSNLVGALHIQPFKAHCL 65

Query: 68  GVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFVFLA 127
           G LM LL HSGFF QT  +N Q    VKY LT +SR LL  N      T  + +  + L 
Sbjct: 66  GRLMSLLVHSGFFVQTHDENEQE---VKYTLTSTSRLLLRHN------TPFEHISLLLLI 125

Query: 128 LHQAMMAPWETMSSWLRTAD---GSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLI 187
            ++AMMA +ETMSSW R+ D     AFET + KS+W+Y+A EPD  N+ ++++ YDS LI
Sbjct: 126 FNKAMMASYETMSSWFRSTDERHSCAFETTNDKSVWDYVAQEPDFSNILNKAMMYDSQLI 185

Query: 188 GRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLP------QQTTKG 247
           GR+VTR ECR VF+G+KSLVDV G+RG +A AIAEAFPHITC V DLP      QQTT+ 
Sbjct: 186 GRVVTR-ECRRVFKGLKSLVDVGGNRGFMAKAIAEAFPHITCIVFDLPHVVDNLQQTTEN 245

Query: 248 LNLAI------QIPPTDAVLLKSVLHQCNDEESIQILKKCKDAI-SKGKGGKVIIIEAVL 307
           LN         +IPP +AVLLK VLH  NDE+SI+ILK CKDAI S+ +GGKVIIIE V 
Sbjct: 246 LNFIAGNMFEAKIPPANAVLLKWVLHDWNDEKSIKILKNCKDAIPSRAEGGKVIIIEMVT 305

Query: 308 ERQMEDEESTETELCGNALMMATFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIEV 353
           E Q+ED+ES ET+LC +   M+     +RNE EWK+LF+AAGFS+YKI + LGLRSLIEV
Sbjct: 306 ENQIEDKESIETQLCEDVFTMSVVTGRERNESEWKNLFLAAGFSDYKIISMLGLRSLIEV 357

[Close]

BLAST of Cp4.1LG03g08830 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.5e-38
Identity = 119/373 (31.90%), Postives = 187/373 (50.13%), Query Frame = 0


Query: 4   KNTDELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNH--GQPMTLSELVEALHIQP 63
           K  DE  +A   IW + F + +  + KC I+L +P+ I NH   QP+TL+EL  A+   P
Sbjct: 21  KVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP 80

Query: 64  SKAHCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQAL 123
           S    L  +MR L H G F +   ++        Y  TP SR L+   +T     +L   
Sbjct: 81  SH---LRRIMRFLVHQGIFKEIPTKDGL---ATGYVNTPLSRRLM---ITRRDGKSL--A 140

Query: 124 PFVFLALHQAMMAPWETMSSWLRT-ADGSA---FETVHGKSIWNYIADEPDLRNLFHQSV 183
           PFV       M+APW  +SS + +  +GS    F+ VHGK +W++  D P L ++ ++++
Sbjct: 141 PFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAM 200

Query: 184 AYDSHLIGRIVTR--PECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ-- 243
           A D+    R+V R    C  +F+GV ++VDV G  G     + + FP I     DLP   
Sbjct: 201 ACDAR---RVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI 260

Query: 244 ---------QTTKGLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKV 303
                    +  +G ++   IP  DA+ +K VLH   D++ I+ILK CK+A+     GKV
Sbjct: 261 EVAEVLDGVENVEG-DMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI-GKV 320

Query: 304 IIIEAVLERQME-------DEESTETELCGNALMMA-TFNNTKRNEREWKDLFMAAGFSN 350
           +I+E+V+    +       DE+     L  + +MMA T    +R  +EW  +   AGF+ 
Sbjct: 321 LIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFAR 377

[Close]

BLAST of Cp4.1LG03g08830 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.4e-28
Identity = 104/365 (28.49%), Postives = 163/365 (44.66%), Query Frame = 0


Query: 7   DELIEAQAHIWNHTFKYINSMSLKCVIELGVPDIIHNH--GQPMTLSELVEALHIQPSKA 66
           DE  +A   IW + F + +  + KC I+L +P+ I NH   QP+TLSEL  A+   PS  
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSH- 69

Query: 67  HCLGVLMRLLHHSGFFSQTQHQNHQHQEVVKYGLTPSSRFLLSPNMTTSTTTTLQALPFV 126
             L  +MR L H G F +   ++                      + T  T T       
Sbjct: 70  --LRRIMRFLVHQGLFKEVPTKD---------------------GLATGYTNT------- 129

Query: 127 FLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLI 186
              L + MM                    +HGK +W +  D      L ++++A D+   
Sbjct: 130 --PLSRRMM-----------------ITKLHGKDLWAFAQDNLCHSQLINEAMACDAR-- 189

Query: 187 GRIVTR--PECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQ-----QTTK 246
            R+V R    C+ +F+GV ++VDV G  G     + + FP I     DLP      Q   
Sbjct: 190 -RVVPRVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLD 249

Query: 247 GL-----NLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLE 306
           G+     ++   IP +DAV++K VLH   D++ I+ILK CK+A+     GKV+I+E V+ 
Sbjct: 250 GVENVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNI-GKVLIVECVIG 309

Query: 307 R-------QMEDEESTETELCGNALMMA-TFNNTKRNEREWKDLFMAAGFSNYKITTFLG 350
                   +  D++     L  + +MM  T    +R  +EW  +   AGF+ Y++  F  
Sbjct: 310 EKKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDD 320

[Close]

BLAST of Cp4.1LG03g08830 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 100.1 bits (248), Expect = 3.7e-21
Identity = 91/346 (26.30%), Postives = 162/346 (46.82%), Query Frame = 0


Query: 27  MSLKCVIELGVPDII--HNHGQPMTLSELVEALHIQPSKAHC---LGVLMRLLHHSGFFS 86
           M LK  +ELGV D I   ++G  ++ SE+  +L  +P+       L  ++RLL       
Sbjct: 44  MVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPEAPVLLDRMLRLLVSHSILK 103

Query: 87  QTQHQNHQHQEVVK----YGLTPSSRFLLSPNMTTSTTTTLQALPFVFLALH-QAMMAPW 146
               ++ ++ +  K    Y   P  ++ L  +  + + ++L       L LH Q ++  W
Sbjct: 104 CCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSGSLSSL------LLLLHSQVILKTW 163

Query: 147 ETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDSHLIGRIVTRPECRDV 206
             +   +      AF + H   ++ YI+ +     LFH++++  S ++ + V   E R  
Sbjct: 164 TNLKDVILEGK-DAFSSAHDMRLFEYISSDDQFSKLFHRAMSESSTMVMKKVLE-EYRG- 223

Query: 207 FEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTKGL----------NLAIQIP 266
           FE V +LVDV G  G I   I   +PHI     DL Q  T+            ++ I++P
Sbjct: 224 FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQVLTQAPFYPGVKHVSGDMFIEVP 283

Query: 267 PTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVLERQMEDEESTETELC 326
             DA+ +K +LH   DE+ I+ILK C  ++   + GKVII+E +   + +  + +   + 
Sbjct: 284 KGDAIFMKWILHDWGDEDCIKILKNCWKSLP--EKGKVIIVEMITPMEPKPNDFSCNTVL 343

Query: 327 GNALMMAT--FNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLIE 351
           G  L+M T      +R+  ++++L  A+GF   +I       S+IE
Sbjct: 344 GMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICLSYSYSVIE 378

[Close]

BLAST of Cp4.1LG03g08830 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 99.8 bits (247), Expect = 4.8e-21
Identity = 68/244 (27.87%), Postives = 123/244 (50.41%), Query Frame = 0


Query: 122 PFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDS 181
           P+V     +A    W  ++  ++    SA+E  +   I+ Y+    +L+ +F++S+   +
Sbjct: 123 PYVLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHT 182

Query: 182 HLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTK---- 241
            ++ + +   E    FEGV   VDV G  G     I   +PHI     DLP    +    
Sbjct: 183 SIVMKKIL--ENYIGFEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQI 242

Query: 242 ------GLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVL 301
                 G ++  +IP  + +L+K +LH  NDE+ ++ILK CK A+   + G++I+IE ++
Sbjct: 243 HGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALP--ETGRIIVIEMIV 302

Query: 302 ERQM-EDEESTETELCGNALMMA-TFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLI 354
            R++ E + +T+  L  +  MM+ T    +R ++E++DL   AGF   KI        +I
Sbjct: 303 PREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWII 362

[Close]

BLAST of Cp4.1LG03g08830 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-20
Identity = 68/244 (27.87%), Postives = 122/244 (50.00%), Query Frame = 0


Query: 122 PFVFLALHQAMMAPWETMSSWLRTADGSAFETVHGKSIWNYIADEPDLRNLFHQSVAYDS 181
           P+V     +A    W  +   ++    SA+E  +   I+ Y+    +L+ +F++S+   +
Sbjct: 123 PYVLAGCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLKKIFNESMTNHT 182

Query: 182 HLIGRIVTRPECRDVFEGVKSLVDVAGDRGIIANAIAEAFPHITCTVLDLPQQTTK---- 241
            ++ + +   E    FEGV   VDV G  G     I   +PHI     DLP    +    
Sbjct: 183 SIVMKKIL--ENYIGFEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLPHIVKEAPQI 242

Query: 242 ------GLNLAIQIPPTDAVLLKSVLHQCNDEESIQILKKCKDAISKGKGGKVIIIEAVL 301
                 G ++  +IP  + +L+K +LH  NDE+ ++ILK CK A+   + G++I+IE ++
Sbjct: 243 HGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALP--ETGRIIVIEMIV 302

Query: 302 ERQM-EDEESTETELCGNALMMA-TFNNTKRNEREWKDLFMAAGFSNYKITTFLGLRSLI 354
            R++ E + +T+  L  +  MM+ T    +R ++E++DL   AGF   KI        +I
Sbjct: 303 PREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPKIIYGAYSYWII 362

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g08830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	5.8e-88	47.55	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	2.8e-82	46.22	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
Q6VMW0	3.2e-78	43.90	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
A0A0N9HMN6	6.2e-74	45.86	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
Q84KK4	2.4e-73	42.12	Isoflavone 4'-O-methyltransferase OS=Lotus japonicus OX=34305 GN=HI4'OMT PE=1 SV...	[more]

BLAST of Cp4.1LG03g08830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023520750.1	5.85e-259	100.00	probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo] >XP_023528579.1 prob...	[more]
KAG6581634.1	2.55e-251	97.46	putative O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022935256.1	9.94e-249	96.33	probable O-methyltransferase 3 [Cucurbita moschata]	[more]
XP_022982782.1	1.83e-242	95.21	probable O-methyltransferase 3 [Cucurbita maxima]	[more]
XP_038905418.1	6.47e-169	70.28	probable O-methyltransferase 3 [Benincasa hispida]	[more]

BLAST of Cp4.1LG03g08830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F418	4.81e-249	96.33	probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442193 PE=...	[more]
A0A6J1IXH8	8.86e-243	95.21	probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481540 PE=3 ...	[more]
A0A6J1DFY3	2.06e-156	65.58	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111020151 PE...	[more]
A0A6J1E096	2.51e-147	61.52	probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE...	[more]
A0A6J1DHJ0	7.78e-137	58.56	trans-resveratrol di-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=...	[more]

BLAST of Cp4.1LG03g08830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	1.5e-38	31.90	O-methyltransferase family protein	[more]
AT4G35150.1	1.4e-28	28.49	O-methyltransferase family protein	[more]
AT1G77530.1	3.7e-21	26.30	O-methyltransferase family protein	[more]
AT1G51990.1	4.8e-21	27.87	O-methyltransferase family protein	[more]
AT1G51990.2	1.1e-20	27.87	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 115..353 e-value: 3.6E-57 score: 195.3
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 26..352 e-value: 1.6E-60 score: 202.5
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 5..353
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 27..74 e-value: 1.6E-16 score: 60.0
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 1..105 e-value: 4.7E-41 score: 140.7
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..353 e-value: 1.3E-98 score: 328.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 5..353
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 17..353 score: 51.25526
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 136..233 e-value: 5.8E-15 score: 55.2 coord: 244..335 e-value: 9.9E-19 score: 67.5
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 121..351
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 6..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g08830.1	Cp4.1LG03g08830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity