Cp4.1LG03g08120 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g08120
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Dihydroorotase
Location	Cp4.1LG03: 2960743 .. 2964262 (-)
RNA-Seq Expression	Cp4.1LG03g08120
Synteny	Cp4.1LG03g08120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTTGAAAAGACTAATGTAGTCATATACAAATGGGAAAGTTTAGGGCGATTTTCGTAATTTAGCAAAAGAAGTGATTCCAAAGCTCGTCTCTCTGTTTGGGCGGCGTAACCCAGCTACGAGCAGGGGAACTGGTGGCTCTCCAACTCCGGTGGATTCAACCCCTCACGGCAGCAGATCTGCAAGTGGCATGTGACCTACGAACAGCTCGTGCCACCCCCCAAGGCGCAACGACTCCCAGTTTATCTTCTCTGGCATACGAGCGACGGCGGCGCTAGGTAACCTACGCAAATAGATCAACGACGGGGGAACTAGATAATGCATCTGTTATTCTGATTTTATATTTTCATTTGAACAGAAGAAAAATCGTTTTAAAGAAGACTCAAGGTTGTCTCTGGATTTTACTATGACAAAGACTTTGGCCCTTCCGTTCCAAGTACATATGCGTTTATTATTGATAGAATCCATTCTATTTATGTTCAATTGGTACTCGGTACTTCATTTTGTTACCATCATTTGTTCTTCAATCAACATTAGTGATATCTTATTCGAGCATTATAGGATGTAATTTGAACATTATCGTGTTGTGTTATATTTTTATAGTTCCCTTAATCTGCTAAGAACTATATCAGTAACAATGAACTAAACTTGATTGAATTCAAATCTTTGCTTTCCATTCATTGATTGATACATTCTTTATGGTAGCCACTGAAATCACCTGTGCAAAAGTTTGAAGGACTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAGCCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGGTAAATTTATCATGTCTATTCTAAATGATTCTATTTTAGCATTCATTCATGGCTTTGATACTGTGAGATCCCACATCGGTTAGGGAGGAGAACGAAACACATTCTACATCAGTTGGGGAGGAGAACGAAACACCCTTTATAAGGGTGTGGAAACCTTTCTTTAAGAGACGCGTTTTAAAAACCTTGAGGGGAATTCCGAAAGGGAAAGCCCAAAGAGGATAATATCTACTAGCAGTAGGCTTGGGTCGTTAAGATACCATATTAAATAAACGAGTTTTCTTCTAAAGCTAATTGATAATGAGATGATTAACTCATGTATCTTGTATAAGATTATGAGTTTTCTTCATCTTTCCTATGTGGGATCTTTTAACATTTTCTGTTCATTCTTGTACTGAACTATTAATGTGCAGTGCAAGTAACTTTGGAAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACTACCACAGCTGCTGCTGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGCCCCAACGAGATCAAGCTTGCCAGTAAGTCTTAGCTTCCAACATTAATTCTTGGAAGATATTTTCCATTGAACTTCATATTTAGTGATTCTCTGTGGTTATATTCTATTTTTTAAACATGATGATGCATTGGCAGGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCCGTCCTCGAGGAGATGGTTGAACATGATATGCCTCTGTTGGTACTTTTTCTGATAGAACACTTTATTTCTCCAATATATAGAGAATCAGTCCTCGGGTTTTCCTTTTAATTTAGAAAGAACAATCTTTGTAGGTTCATGGCGAGGTAACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGTATACATCATTATTTTCGTTGAAGTGTTTTGGTTAGTTATCGCTCTTTTATTCCGTTCTTCAACAGTGGTATCACTTTTCGTTATAACGTAGAATTACTAGTTTATGAGTTTTAAAATACTTCACAGGACTTTTAGCAGCAACAGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGTATGTTCCAACTAAATTTTGTGCTGATTCTTTATATTTTGGTCTTCTTGTTTGTCTGTGTCAATAGCATTCGTGAAAAATAATCTGTAATGGCTTAGGCTCACCGCTAGGAGATGTTGCCCTCTTTGGGCTTTCTCTTTTGGGCTTTTCCTTAAGATTTTTAAAACGCGTCTGCTAGGGAAAGGTTTCCACACCCTTATAAATGGTGTTTCGTTCTCCTTCCCAACCAATGTGGAATATCACATAATCTCCTAACAATTATCAGATATTCCTATTGAAGTGGGAAGCTTTTTGGAGGTTAGAATGGATTAATCTCAACAGACGCTGGCTTTTGACTTTTTGTAATTGATCTTAGAATCACAATAGTTGATCTAGTGATCAATAAGGCTTTGAGCGAATGGGTTCAAGTGATAGTAGCAAGCCACCAAAGATTTAATACCTTACGAGTTTCCTTGCGTAGAAGGGGCTTGATAGTTATCTTGAGACGATGATATTCGAGATGTGTATTAGCTGACCCAGATACTCACGAATATAAATTATTGGCGACTTTTCACTATGAAAATCGAGAATCTCCCCTAAATGAAAGCTCTCGTCCTCTCATATAGGGCAGGCAATTGTGTCAGCTGTGACCAGTGGGAGCAAAAAGTTTTTTCTTGGAACGGATAGCGCTCCACACGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCTATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGTATACTCTTCATCCTCATATGCTAATAATTTGTTCACGCCTTCATTTTTATCAAATCATCACTCTTTTTGTTCCCTTTTTCTGTGCCAAGGCGTGTGCTCTTGACAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACACCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTCCCCGAGTCATTTTCTTTCCCCTTTGGGGACATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAAGACTAACAAGTTCCACTGTTGCTCACACAATATAACACCACATTCTAGACATCCTCCTTTCTCTGATCATCTAGACCCATCTTGATTTCCCATGATATCAACTTTTTTGGATCTTTGATATTGTTGGTTTGCTAATAAAATTCCCAAATTTTGTTTTTGGGACTTCATTAACTCGCATATGAACGATTTTGTTTTAAGTTTCGTTTGTATTAATGTTTTTTTTAGTTTAACATGCGAGAGTAAAAAGATTCAAACTTTTGATATATGAAATAGG

mRNA sequence

GAGTTGAAAAGACTAATGTAGTCATATACAAATGGGAAAGTTTAGGGCGATTTTCGTAATTTAGCAAAAGAAGTGATTCCAAAGCTCGTCTCTCTGTTTGGGCGGCGTAACCCAGCTACGAGCAGGGGAACTGGTGGCTCTCCAACTCCGGTGGATTCAACCCCTCACGGCAGCAGATCTGCAAGTGGCATGTGACCTACGAACAGCTCGTGCCACCCCCCAAGGCGCAACGACTCCCAGTTTATCTTCTCTGGCATACGAGCGACGGCGGCGCTAGAAGAAAAATCGTTTTAAAGAAGACTCAAGGTTGTCTCTGGATTTTACTATGACAAAGACTTTGGCCCTTCCGTTCCAACCACTGAAATCACCTGTGCAAAAGTTTGAAGGACTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAGCCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACTACCACAGCTGCTGCTGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGCCCCAACGAGATCAAGCTTGCCAGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCCGTCCTCGAGGAGATGGTTGAACATGATATGCCTCTGTTGGTTCATGGCGAGGTAACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGACTTTTAGCAGCAACAGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTGACCAGTGGGAGCAAAAAGTTTTTTCTTGGAACGGATAGCGCTCCACACGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCTATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGCGTGTGCTCTTGACAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACACCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTCCCCGAGTCATTTTCTTTCCCCTTTGGGGACATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAAGACTAACAAGTTCCACTGTTGCTCACACAATATAACACCACATTCTAGACATCCTCCTTTCTCTGATCATCTAGACCCATCTTGATTTCCCATGATATCAACTTTTTTGGATCTTTGATATTGTTGGTTTGCTAATAAAATTCCCAAATTTTGTTTTTGGGACTTCATTAACTCGCATATGAACGATTTTGTTTTAAGTTTCGTTTGTATTAATGTTTTTTTTAGTTTAACATGCGAGAGTAAAAAGATTCAAACTTTTGATATATGAAATAGG

Coding sequence (CDS)

ATGACAAAGACTTTGGCCCTTCCGTTCCAACCACTGAAATCACCTGTGCAAAAGTTTGAAGGACTCAAGTTCATAAGGCGTCAGGGGCCAAAAATGCAGCTCACACTTACTCAGCCTGATGATTGGCATCTTCATCTTCGTGATGGCGATCTTTTAAAAGCTGTTCTCCCTCACAGGGCTATTGTGATGCCAAATTTGAAGCCACCCATCACTACCACAGCTGCTGCTGTGGCTTATCGAGATTCTATCTTGGAAGCATTACCGTCCAATAGCAACTTCACTCCTCTTATGACCCTTTATTTGACAGATACAATGAGCCCCAACGAGATCAAGCTTGCCAGAAGAAGTGGGGCTGTTTATGCTGTAAAATTGTATCCTGCTGGTGCTACAACCAATTCCCAAGACGGTGTAACAGATCTTTTCGGAAAGTGTCTGCCCGTCCTCGAGGAGATGGTTGAACATGATATGCCTCTGTTGGTTCATGGCGAGGTAACAAATCCTGATGTCGACGTGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCTCTGATTGAGAGGCTTCCAAAACTGAAGGTGGTGATGGAGCATATCACTACTGCAGATGCTGCTAAGTTTGTTCAGTCTTGCGAAAATGGACTTTTAGCAGCAACAGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTGTTTCAAGGAGGATTGCAGCCACATAATTACTGTCTTCCAGTACTCAAGAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTGACCAGTGGGAGCAAAAAGTTTTTTCTTGGAACGGATAGCGCTCCACACGATCGACGAAGGAAAGAATGTGCTTGTGGTTGTGCTGGTATATACAGTGCCCCTATTGCCTTATCATTATACGCAAAGGTGTTTGAAGAGGCGTGTGCTCTTGACAAATTAGAAGCTTTTACAAGTTTCAATGGACCAGACTTCTATGGGCTCCCCAGAAACACCTCAAAAATTACACTGCAAAAAGCTCCATGGAAGGTCCCCGAGTCATTTTCTTTCCCCTTTGGGGACATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCATCAACTAA

Protein sequence

MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQPCIN

Homology

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BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot
Match: O04904 (Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 1.7e-167
Identity = 286/375 (76.27%), Postives = 326/375 (86.93%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFE-GLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH- 60
           M KTL  P+    S   KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL AV+PH 
Sbjct: 1   MIKTLVSPYSGFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLHAVVPHS 60

Query: 61  -----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLA 120
                RAIVMPNLKPP+T+TAAA+ YR  I++ALPS S+F PLMTLYLTD   P EI+LA
Sbjct: 61  ASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLPEEIRLA 120

Query: 121 RRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFD 180
           R SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+ +MPLLVHGEVT+P +DVFD
Sbjct: 121 RESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDPSIDVFD 180

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSL 240
           REK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV+SC+ G + ATVTPQHLLLNRN+L
Sbjct: 181 REKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLLLNRNAL 240

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYS 300
           FQGGLQPHNYCLPVLKRE HR+AIV AVTSGSKKFFLGTDSAPH+R RKE +CGCAGIYS
Sbjct: 241 FQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCGCAGIYS 300

Query: 301 APIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFG 360
           APIALSLYAKVF+EA ALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PWKVP+ F+FPFG
Sbjct: 301 APIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDVFNFPFG 360

Query: 361 DIVPMFAGETLEWQP 369
           +IVPMFAGETL+WQP
Sbjct: 361 EIVPMFAGETLQWQP 375

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BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot
Match: Q0JJD1 (Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC PE=2 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 6.0e-160
Identity = 267/345 (77.39%), Postives = 304/345 (88.12%), Query Frame = 0


Query: 30  PKMQLTLTQPDDWHLHLRDGDLLKAVLPH------RAIVMPNLKPPITTTAAAVAYRDSI 89
           P  +LT+T+PDDWHLHLR+GD+L AVLPH      RAIVMPNLKPP+TTTA A+ YR+ I
Sbjct: 56  PLQELTITRPDDWHLHLREGDVLAAVLPHSAMHFGRAIVMPNLKPPVTTTARALEYREEI 115

Query: 90  LEALPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGK 149
           L ALP  SNF PLMTLYLTD  SP EIKLA++SG V+AVKLYP+GATTNSQDGVTD+FGK
Sbjct: 116 LRALPPGSNFVPLMTLYLTDNTSPEEIKLAKKSGVVFAVKLYPSGATTNSQDGVTDIFGK 175

Query: 150 CLPVLEEMVEHDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTA 209
           CLPVLEEM   +MPLLVHGEVT+  VD FDREKVFIE +L PL++RLP+LK+VMEHITT 
Sbjct: 176 CLPVLEEMARQEMPLLVHGEVTDQHVDTFDREKVFIEKILAPLVQRLPQLKIVMEHITTM 235

Query: 210 DAAKFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 269
           DA  FV+SC+ G +AATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRETHRQAIVSAVTS
Sbjct: 236 DAVNFVESCKEGHVAATVTPQHLLLNRNALFQGGLQPHNYCLPVLKRETHRQAIVSAVTS 295

Query: 270 GSKKFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGP 329
           GSK++FLGTDSAPHD+RRKEC+CGCAGIYSAP+ALSLYAKVFE+A ALDKLEAFTSFNGP
Sbjct: 296 GSKQYFLGTDSAPHDKRRKECSCGCAGIYSAPVALSLYAKVFEQAGALDKLEAFTSFNGP 355

Query: 330 DFYGLPRNTSKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQP 369
           DFYGLPRNTSKI L+K+ WKVP+++S+  G+IVPMF G TLEW P
Sbjct: 356 DFYGLPRNTSKIVLRKSAWKVPDTYSYSSGEIVPMFTGNTLEWLP 400

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BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot
Match: B1XI96 (Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=pyrC PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.0e-123
Identity = 221/341 (64.81%), Postives = 260/341 (76.25%), Query Frame = 0


Query: 33  QLTLTQPDDWHLHLRDGDLLKAVLPH------RAIVMPNLKPPITTTAAAVAYRDSILEA 92
           ++TLT+PDDWHLHLRDG+ LKAVLP       RAIVMPNLKPP+ T A A AY D IL A
Sbjct: 3   KITLTRPDDWHLHLRDGEALKAVLPDTVRQFARAIVMPNLKPPVRTVAEAAAYHDRILAA 62

Query: 93  LPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 152
           +P    F PLMTLYLTD  SP EI+ A+ SG V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  IPPGRTFEPLMTLYLTDNTSPAEIQAAKESGFVKAVKYYPAGATTNSDLGVTDI-RKCDA 122

Query: 153 VLEEMVEHDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 212
           V E M   D+PLL+HGEVT+  VDVFDREKVFIET L+PL ER P+L++V+EHITT DA 
Sbjct: 123 VFEAMQTVDLPLLLHGEVTDHRVDVFDREKVFIETYLRPLKERFPQLRIVLEHITTKDAV 182

Query: 213 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 272
           +FV + +   +AAT+TPQHLL NRNS+FQGG++PH YCLP+LKRETHR+A++ A TSG+ 
Sbjct: 183 EFVLASDE-KVAATITPQHLLFNRNSIFQGGIRPHFYCLPILKRETHREALLEAATSGNP 242

Query: 273 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 332
           KFFLGTDSAPH R RKE  CGCAG YSA  AL LYA VFE A ALDKLE F SF GPDFY
Sbjct: 243 KFFLGTDSAPHGRDRKESDCGCAGCYSALHALELYATVFEAADALDKLEGFASFYGPDFY 302

Query: 333 GLPRNTSKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 368
            LPRNT KITL K PW++P++  FP   +VP++AG+ L W+
Sbjct: 303 QLPRNTEKITLTKTPWQIPDALPFPESSLVPLWAGQELSWK 341

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot
Match: C1D672 (Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.6e-120
Identity = 221/341 (64.81%), Postives = 255/341 (74.78%), Query Frame = 0


Query: 34  LTLTQPDDWHLHLRDGDLLKAVLPH------RAIVMPNLKPPITTTAAAVAYRDSILEAL 93
           LTLT+PDDWHLHLRDG+ L AV  +      RAIVMPNLKPP+TT   A AYR+ IL AL
Sbjct: 4   LTLTRPDDWHLHLRDGEALAAVAAYSAREFARAIVMPNLKPPVTTVVQAAAYRERILAAL 63

Query: 94  -PSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 153
            P    F PLMTLYLTD   P EI     SG V+A+KLYPAGATTNS  GVT L G CLP
Sbjct: 64  PPGGPAFEPLMTLYLTDNTPPAEIDRVAESGFVHALKLYPAGATTNSDAGVTSLSG-CLP 123

Query: 154 VLEEMVEHDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 213
            L  M E  +PLLVHGEVT+P +DVFDRE VFI+TVL+PL+  LP L+VVMEHITT  AA
Sbjct: 124 TLTRMAELGVPLLVHGEVTDPQIDVFDREAVFIDTVLQPLLAELPTLRVVMEHITTRQAA 183

Query: 214 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 273
           +FV +  +  +AATVTPQH+L+NRN+LF GGL+PH+YCLPVLKRE HR AI  AVTSGS 
Sbjct: 184 EFVLAAPDN-IAATVTPQHMLMNRNALFTGGLRPHHYCLPVLKREEHRAAIARAVTSGST 243

Query: 274 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 333
           +FFLGTDSAPH R  KE ACGCAGI+SA  AL LYA+ FE+A ALD+LEAF SFNGPDFY
Sbjct: 244 RFFLGTDSAPHARHAKEAACGCAGIFSAHAALPLYAEAFEDAGALDRLEAFASFNGPDFY 303

Query: 334 GLPRNTSKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 368
            LPRNT  +TL++  W VP S  +    +VP+ AGETL WQ
Sbjct: 304 RLPRNTGTVTLKREEWTVPASLPYGNDTLVPLRAGETLRWQ 342

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot
Match: B0JQJ1 (Dihydroorotase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=pyrC PE=3 SV=1)

HSP 1 Score: 430.6 bits (1106), Expect = 1.8e-119
Identity = 215/341 (63.05%), Postives = 254/341 (74.49%), Query Frame = 0


Query: 33  QLTLTQPDDWHLHLRDGDLLKAVLPH------RAIVMPNLKPPITTTAAAVAYRDSILEA 92
           +LT+T+PDDWHLHLRDG  +KAVLPH      RAI+MPNLKPP+ + A A +YR+ IL A
Sbjct: 3   RLTMTRPDDWHLHLRDGAAMKAVLPHTVRQFARAIIMPNLKPPVRSVADAASYRERILAA 62

Query: 93  LPSNSNFTPLMTLYLTDTMSPNEIKLARRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 152
           +P    F PLMTLYLTD  SP EI  A+ S  V AVK YPAGATTNS  GVTDL  +C  
Sbjct: 63  VPEGQQFEPLMTLYLTDNTSPEEIVAAKASQFVKAVKYYPAGATTNSDLGVTDL-RRCDG 122

Query: 153 VLEEMVEHDMPLLVHGEVTNPDVDVFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 212
           VL  M + DMPLL+HGEVT+ D+DVFDREKVFIE  L PLI R PKL+VV EHITTADA 
Sbjct: 123 VLAAMEQVDMPLLLHGEVTDGDIDVFDREKVFIEKHLIPLITRFPKLRVVFEHITTADAV 182

Query: 213 KFVQSCENGLLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 272
           KFV S  N  +AAT+TPQHLL +RN LF GG++PH YCLP+LKRE HR A++ A TSG+ 
Sbjct: 183 KFVLSANNN-VAATITPQHLLFSRNILFTGGIRPHFYCLPILKREDHRLALLQAATSGNP 242

Query: 273 KFFLGTDSAPHDRRRKECACGCAGIYSAPIALSLYAKVFEEACALDKLEAFTSFNGPDFY 332
           KFFLGTDSAPH R  KE +CGCAG YSA  AL LYA+ FE   A+D+LE F SF+GPDFY
Sbjct: 243 KFFLGTDSAPHSRYSKESSCGCAGCYSALHALELYAEAFESVDAIDQLEGFASFHGPDFY 302

Query: 333 GLPRNTSKITLQKAPWKVPESFSFPFGDIVPMFAGETLEWQ 368
            LPRNT +ITL K+PW++P+   FP   +VP+ AGE + WQ
Sbjct: 303 QLPRNTEQITLTKSPWRIPDELPFPESGLVPLRAGEEITWQ 341

[Close]

BLAST of Cp4.1LG03g08120 vs. NCBI nr
Match: XP_023528165.1 (dihydroorotase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753 bits (1944), Expect = 2.08e-274
Identity = 371/377 (98.41%), Postives = 371/377 (98.41%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPCIN
Sbjct: 361 IVPMFAGETLEWQPCIN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. NCBI nr
Match: XP_022935161.1 (dihydroorotase, mitochondrial [Cucurbita moschata])

HSP 1 Score: 748 bits (1930), Expect = 2.83e-272
Identity = 368/377 (97.61%), Postives = 370/377 (98.14%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTM+PNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMNPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVDVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC+N
Sbjct: 361 IVPMFAGETLEWQPCMN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. NCBI nr
Match: XP_022983807.1 (dihydroorotase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 745 bits (1924), Expect = 2.33e-271
Identity = 368/377 (97.61%), Postives = 368/377 (97.61%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTLALPFQPLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLALPFQPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVDVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRN SKITLQKAPWKVPESFSFPFGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPCIN
Sbjct: 361 IVPMFAGETLEWQPCIN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. NCBI nr
Match: XP_038897131.1 (dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida])

HSP 1 Score: 726 bits (1875), Expect = 6.86e-264
Identity = 357/377 (94.69%), Postives = 364/377 (96.55%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTL LPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLGLPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVD+FDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCE+GLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCEHGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKEC+CGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKECSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEE  ALDKLEAFTSFNGPDFYGLPRNTSKITL KAPW+VP+SFSFPFGD
Sbjct: 301 PIALSLYAKVFEEVGALDKLEAFTSFNGPDFYGLPRNTSKITLHKAPWQVPKSFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC N
Sbjct: 361 IVPMFAGETLEWQPCFN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. NCBI nr
Match: XP_008443394.1 (PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 714 bits (1842), Expect = 7.92e-259
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTL LPFQPLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           +SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVD+FDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPW+VP+SF+FPFGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC N
Sbjct: 361 IVPMFAGETLEWQPCFN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL
Match: A0A6J1F3S4 (Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 1.37e-272
Identity = 368/377 (97.61%), Postives = 370/377 (98.14%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTM+PNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMNPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVDVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC+N
Sbjct: 361 IVPMFAGETLEWQPCMN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL
Match: A0A6J1J0C7 (Dihydroorotase OS=Cucurbita maxima OX=3661 GN=LOC111482314 PE=3 SV=1)

HSP 1 Score: 745 bits (1924), Expect = 1.13e-271
Identity = 368/377 (97.61%), Postives = 368/377 (97.61%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTLALPFQPLKSP QKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLALPFQPLKSPRQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVDVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRN SKITLQKAPWKVPESFSFPFGD
Sbjct: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNASKITLQKAPWKVPESFSFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPCIN
Sbjct: 361 IVPMFAGETLEWQPCIN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL
Match: A0A1S3B7W2 (Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 3.83e-259
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTL LPFQPLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           +SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVD+FDR
Sbjct: 121 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPW+VP+SF+FPFGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC N
Sbjct: 361 IVPMFAGETLEWQPCFN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL
Match: A0A5D3DPM6 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007860 PE=3 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 9.82e-258
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTL LPFQPLKSP QKFEG+KFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 88  MTKTLVLPFQPLKSPAQKFEGVKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 147

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSIL+ALPSNSNF PLMTLYLTDTMSPNEIKLAR
Sbjct: 148 STFGRAIVMPNLKPPITTTAAAVAYRDSILKALPSNSNFIPLMTLYLTDTMSPNEIKLAR 207

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           +SGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE DMPLLVHGEVTNPDVD+FDR
Sbjct: 208 KSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNPDVDMFDR 267

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLIERLPKLKVVMEHITTA+AAKFV SCE+ LLAATVTPQHLLLNRNSLF
Sbjct: 268 EKVFIETVLKPLIERLPKLKVVMEHITTANAAKFVLSCEHALLAATVTPQHLLLNRNSLF 327

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPH+RRRKE +CGCAGIYSA
Sbjct: 328 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHERRRKESSCGCAGIYSA 387

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPW+VP+SF+FPFGD
Sbjct: 388 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFTFPFGD 447

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC N
Sbjct: 448 IVPMFAGETLEWQPCFN 464

[Close]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL
Match: A0A6J1CTX5 (Dihydroorotase OS=Momordica charantia OX=3673 GN=LOC111014187 PE=3 SV=1)

HSP 1 Score: 707 bits (1826), Expect = 9.76e-257
Identity = 348/377 (92.31%), Postives = 358/377 (94.96%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFEGLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH-- 60
           MTKTL L FQPLKSPVQKFEGLKF++RQ PKMQLTLTQPDDWHLHLRDGDLLKAVLPH  
Sbjct: 1   MTKTLVLTFQPLKSPVQKFEGLKFLKRQAPKMQLTLTQPDDWHLHLRDGDLLKAVLPHSA 60

Query: 61  ----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLAR 120
               RAIVMPNLKPPITTTAAAVAYRDSIL+ LPSNSNF PLMTLYLTDTMSPNEIKLAR
Sbjct: 61  SNFGRAIVMPNLKPPITTTAAAVAYRDSILKTLPSNSNFIPLMTLYLTDTMSPNEIKLAR 120

Query: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFDR 180
           RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVE +MPLLVHGEVTNPD+DVFDR
Sbjct: 121 RSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQNMPLLVHGEVTNPDIDVFDR 180

Query: 181 EKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSLF 240
           EKVFIETVLKPLI+RLPKLKVVMEHITTADAA FVQS ++G LAATVTPQHLLLNRNSLF
Sbjct: 181 EKVFIETVLKPLIQRLPKLKVVMEHITTADAANFVQSYKDGFLAATVTPQHLLLNRNSLF 240

Query: 241 QGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYSA 300
           QGGLQPHNYCLPVLKRETHR+AIVSAVTSGSKKFFLGTDSAPH+RRRKEC CGCAGIYSA
Sbjct: 241 QGGLQPHNYCLPVLKRETHREAIVSAVTSGSKKFFLGTDSAPHERRRKECTCGCAGIYSA 300

Query: 301 PIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFGD 360
           PIALSLYAKVFEEA ALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSF FGD
Sbjct: 301 PIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFSFGD 360

Query: 361 IVPMFAGETLEWQPCIN 371
           IVPMFAGETLEWQPC N
Sbjct: 361 IVPMFAGETLEWQPCFN 377

[Close]

BLAST of Cp4.1LG03g08120 vs. TAIR 10
Match: AT4G22930.1 (pyrimidin 4 )

HSP 1 Score: 590.1 bits (1520), Expect = 1.2e-168
Identity = 286/375 (76.27%), Postives = 326/375 (86.93%), Query Frame = 0


Query: 1   MTKTLALPFQPLKSPVQKFE-GLKFIRRQGPKMQLTLTQPDDWHLHLRDGDLLKAVLPH- 60
           M KTL  P+    S   KF+   + ++ +  +M+LT+TQPDDWHLHLRDGDLL AV+PH 
Sbjct: 1   MIKTLVSPYSGFGSQKLKFDRSSEKVKTRAVRMELTITQPDDWHLHLRDGDLLHAVVPHS 60

Query: 61  -----RAIVMPNLKPPITTTAAAVAYRDSILEALPSNSNFTPLMTLYLTDTMSPNEIKLA 120
                RAIVMPNLKPP+T+TAAA+ YR  I++ALPS S+F PLMTLYLTD   P EI+LA
Sbjct: 61  ASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMKALPSESSFDPLMTLYLTDKTLPEEIRLA 120

Query: 121 RRSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEHDMPLLVHGEVTNPDVDVFD 180
           R SG VYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMV+ +MPLLVHGEVT+P +DVFD
Sbjct: 121 RESGVVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVKQNMPLLVHGEVTDPSIDVFD 180

Query: 181 REKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVQSCENGLLAATVTPQHLLLNRNSL 240
           REK+FIETVL+PLI+RLP+LKVVMEHITT DA  FV+SC+ G + ATVTPQHLLLNRN+L
Sbjct: 181 REKIFIETVLQPLIQRLPQLKVVMEHITTMDAVNFVESCKEGSVGATVTPQHLLLNRNAL 240

Query: 241 FQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSKKFFLGTDSAPHDRRRKECACGCAGIYS 300
           FQGGLQPHNYCLPVLKRE HR+AIV AVTSGSKKFFLGTDSAPH+R RKE +CGCAGIYS
Sbjct: 241 FQGGLQPHNYCLPVLKREIHREAIVKAVTSGSKKFFLGTDSAPHERSRKESSCGCAGIYS 300

Query: 301 APIALSLYAKVFEEACALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWKVPESFSFPFG 360
           APIALSLYAKVF+EA ALDKLEAFTSFNGPDFYGLPRN+SKITL+K+PWKVP+ F+FPFG
Sbjct: 301 APIALSLYAKVFDEAGALDKLEAFTSFNGPDFYGLPRNSSKITLKKSPWKVPDVFNFPFG 360

Query: 361 DIVPMFAGETLEWQP 369
           +IVPMFAGETL+WQP
Sbjct: 361 EIVPMFAGETLQWQP 375

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g08120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04904	1.7e-167	76.27	Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1	[more]
Q0JJD1	6.0e-160	77.39	Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC P...	[more]
B1XI96	2.0e-123	64.81	Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=320...	[more]
C1D672	1.6e-120	64.81	Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3...	[more]
B0JQJ1	1.8e-119	63.05	Dihydroorotase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=pyrC PE=...	[more]

BLAST of Cp4.1LG03g08120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528165.1	2.08e-274	98.41	dihydroorotase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022935161.1	2.83e-272	97.61	dihydroorotase, mitochondrial [Cucurbita moschata]	[more]
XP_022983807.1	2.33e-271	97.61	dihydroorotase, mitochondrial isoform X1 [Cucurbita maxima]	[more]
XP_038897131.1	6.86e-264	94.69	dihydroorotase, mitochondrial-like isoform X1 [Benincasa hispida]	[more]
XP_008443394.1	7.92e-259	93.10	PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo]	[more]

BLAST of Cp4.1LG03g08120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F3S4	1.37e-272	97.61	Dihydroorotase OS=Cucurbita moschata OX=3662 GN=LOC111442116 PE=3 SV=1	[more]
A0A6J1J0C7	1.13e-271	97.61	Dihydroorotase OS=Cucurbita maxima OX=3661 GN=LOC111482314 PE=3 SV=1	[more]
A0A1S3B7W2	3.83e-259	93.10	Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1	[more]
A0A5D3DPM6	9.82e-258	93.10	Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00786...	[more]
A0A6J1CTX5	9.76e-257	92.31	Dihydroorotase OS=Momordica charantia OX=3673 GN=LOC111014187 PE=3 SV=1	[more]

BLAST of Cp4.1LG03g08120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22930.1	1.2e-168	76.27	pyrimidin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.20.20.140		coord: 32..368 e-value: 3.4E-122 score: 409.8
IPR006680	Amidohydrolase-related	PFAM	PF01979	Amidohydro_1	coord: 41..344 e-value: 2.6E-23 score: 82.9
IPR004721	Dihydroorotase homodimeric type	TIGRFAM	TIGR00856	TIGR00856	coord: 34..367 e-value: 4.5E-140 score: 464.4
IPR004721	Dihydroorotase homodimeric type	PIRSF	PIRSF001237	Dihydroorotase	coord: 30..370 e-value: 7.0E-141 score: 467.0
IPR004721	Dihydroorotase homodimeric type	PANTHER	PTHR43137	DIHYDROOROTASE	coord: 31..368
IPR004721	Dihydroorotase homodimeric type	HAMAP	MF_00219	PyrC_classII	coord: 32..369 score: 44.970165
IPR004721	Dihydroorotase homodimeric type	CDD	cd01294	DHOase	coord: 34..366 e-value: 0.0 score: 559.208
IPR002195	Dihydroorotase, conserved site	PROSITE	PS00483	DIHYDROOROTASE_2	coord: 271..282
IPR002195	Dihydroorotase, conserved site	PROSITE	PS00482	DIHYDROOROTASE_1	coord: 41..49
IPR032466	Metal-dependent hydrolase	SUPERFAMILY	51556	Metallo-dependent hydrolases	coord: 33..367

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g08120.1	Cp4.1LG03g08120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006207	'de novo' pyrimidine nucleobase biosynthetic process
biological_process	GO:0044205	'de novo' UMP biosynthetic process
biological_process	GO:0019856	pyrimidine nucleobase biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004151	dihydroorotase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016812	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides