Cp4.1LG03g04860 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g04860 vs. ExPASy Swiss-Prot
Match: Q9LJD8 (MAP3K epsilon protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=M3KE1 PE=1 SV=1)

HSP 1 Score: 1756.9 bits (4549), Expect = 0.0e+00
Identity = 991/1529 (64.81%), Postives = 1106/1529 (72.33%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            M+RQ+ ++ FHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LN IM       NLNHKNIVKYLGS KTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNTIMQEIDLLKNLNHKNIVKYLGSSKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYI+Q                            GLVKLADFGVATKL EADVNTHSVV
Sbjct: 121  VAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLNEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            D +PPIPDSLSPDITDFLRQCFKK                        D+RQRPDAKTLL
Sbjct: 301  DDNPPIPDSLSPDITDFLRQCFKK------------------------DSRQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR--KELSSDVVTDMSKSQKI--FASGPN---------- 420
            SHPWI+N RRAL SSLRHSGT++  KE ++    D   SQ      SG N          
Sbjct: 361  SHPWIRNSRRALQSSLRHSGTIKYMKEATASSEKDDEGSQDAAESLSGENVGISKTDSKS 420

Query: 421  ----------------------SVEEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIATS 480
                                    E  ++ EDD + DQVPTLSIHE SS   G+      
Sbjct: 421  KLPLVGVSSFRSEKDQSTPSDLGEEGTDNSEDDIMSDQVPTLSIHEKSSDAKGT------ 480

Query: 481  EPIEFHESHGRA-RDEVIMNGDVPSTELRED-AARQHGEKETSTTSGNSSFGFEPESQDN 540
             P +  + HG++ R E   N    ++E R++ +A +H  KE S     +S  F  + ++ 
Sbjct: 481  -PQDVSDFHGKSERGETPENLVTETSEARKNTSAIKHVGKELSIPVDQTSHSFGRKGEER 540

Query: 541  SFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMGY 600
              +K  K   ++ GNEL++FSD PGDASL DLF PLDK+   +  EASTS+ T   N G 
Sbjct: 541  GIRKAVKTPSSVSGNELARFSDPPGDASLHDLFHPLDKVSEGKPNEASTSMPTSNVNQGD 600

Query: 601  LSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDEK 660
              V D  KNDLATKLRATIAQKQME E G ++ GGDL RL+MGVLKDD IDIDGLVFDEK
Sbjct: 601  SPVADGGKNDLATKLRATIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEK 660

Query: 661  LSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLPL 720
            +  ENLFPLQAVEF RL  SLRPDE ED IVS+CQKL+A+F QRPEQK+V++TQHG LPL
Sbjct: 661  VPAENLFPLQAVEFSRLVSSLRPDESEDAIVSSCQKLVAMFRQRPEQKVVFVTQHGFLPL 720

Query: 721  TELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVP--DRPREVRMEA 780
             +LL++PK+R+IC+VLQLIN+I+KDN DFQENACLVGLIP+VM FA P  DR RE+R EA
Sbjct: 721  MDLLDIPKSRVICAVLQLINEIIKDNTDFQENACLVGLIPVVMSFAGPERDRSREIRKEA 780

Query: 781  AYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTL 840
            AYF QQLCQSS LTLQMF+ACRGIPVLV FLEADYAKYR+MVHLAIDGMWQ+FKL+RST 
Sbjct: 781  AYFLQQLCQSSPLTLQMFIACRGIPVLVGFLEADYAKYREMVHLAIDGMWQVFKLKRSTP 840

Query: 841  RNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIF 900
            RN FCRIAAK+GILLRLINTLYSLNEATRLASI+ G    +DG   R RSGQLDP +PIF
Sbjct: 841  RNDFCRIAAKNGILLRLINTLYSLNEATRLASISGG----LDGQAPRVRSGQLDPNNPIF 900

Query: 901  SQYE-ASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
             Q E +S  + DQPD+LK RHG        G  EPS ASTS+SQRSD +Q D      D 
Sbjct: 901  GQNETSSLSMIDQPDVLKTRHG--------GGEEPSHASTSNSQRSDVHQPD--ALHPDG 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            D+P+ S+   +A  S   ++    ++                            ++NRTS
Sbjct: 961  DKPRVSSVAPDASTSGTEDVRQQHRI--------------------------SLSANRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TD+  KL E  +NGFP     TQ EQVRPLLSLLDKEPPSRH+SGQL+Y++H++G+ERHE
Sbjct: 1021 TDKLQKLAEGASNGFPV----TQTEQVRPLLSLLDKEPPSRHYSGQLDYVKHITGIERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            + +PLLH SNEKK NG+ DFLMAEFA                +VS RGK+NG+LD T++ 
Sbjct: 1081 SRLPLLHGSNEKKNNGDLDFLMAEFA----------------EVSGRGKENGSLDTTTRY 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
              K + KKV   ++ EG ASTSGIASQTASGVLSGSGVLNARPGSATSSGLL+HMVSTL+
Sbjct: 1141 PSKTMTKKV---LAIEGVASTSGIASQTASGVLSGSGVLNARPGSATSSGLLAHMVSTLS 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYL KVADLLLEFA+ADTTVKSYMCSQSLL+RLFQMFNRVEPPILLKIL+C NHL
Sbjct: 1201 ADVAREYLEKVADLLLEFARADTTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIK+LIPNLELK+G LV QIH EVLSALFNLCKINKRRQE AAENG
Sbjct: 1261 STDPNCLENLQRADAIKHLIPNLELKDGHLVYQIHHEVLSALFNLCKINKRRQEQAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLM FI+SDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV AL
Sbjct: 1321 IIPHLMLFIMSDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIAL 1368

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLA DNDNRKVEQALLK+DA+QKLV FFQ CPE+HFVHILEPFLKIITKS RIN 
Sbjct: 1381 DSIAVCLAQDNDNRKVEQALLKQDAIQKLVDFFQSCPERHFVHILEPFLKIITKSYRINK 1368

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1454
            TLAVNGLTPLLI+RLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLP KLQNLIEER
Sbjct: 1441 TLAVNGLTPLLISRLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPQKLQNLIEER 1368

BLAST of Cp4.1LG03g04860 vs. ExPASy Swiss-Prot
Match: Q9SFB6 (MAP3K epsilon protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MAP3KE2 PE=2 SV=1)

HSP 1 Score: 1653.3 bits (4280), Expect = 0.0e+00
Identity = 956/1532 (62.40%), Postives = 1078/1532 (70.37%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            M+RQ+ ++ FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIGQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LN IM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNTIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            V VYI+Q                            GLVKLADFGVATKL EAD NTHSVV
Sbjct: 121  VTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLNEADFNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIE+SGVCAASDIWSVGCT+IELLTCVPPYYDLQPMPAL+RIVQ       
Sbjct: 181  GTPYWMAPEVIELSGVCAASDIWSVGCTIIELLTCVPPYYDLQPMPALYRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            D  PPIPDSLSPDITDFLR CFKK                        D+RQRPDAKTLL
Sbjct: 301  DDTPPIPDSLSPDITDFLRLCFKK------------------------DSRQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR--KELSSDVVTDMSKSQKIF----------------- 420
            SHPWI+N RRAL SSLRHSGT+R  KE  S    D   SQ++                  
Sbjct: 361  SHPWIRNSRRALRSSLRHSGTIRYMKETDSSSEKDAEGSQEVVESVSAEKVEVTKTNSKS 420

Query: 421  ---------------ASGPNSV-EEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIA--- 480
                            S P+ + EE    EDD   DQ PTLS+H+ SS  +G+  I+   
Sbjct: 421  KLPVIGGASFRSEKDQSSPSDLGEEGTDSEDDINSDQGPTLSMHDKSSRQSGTCSISSDA 480

Query: 481  ---TSEPIEFHESHGRARDEVIMNGDVPSTELREDA-ARQHGEKETSTTSGNSSFGFEPE 540
               + + +E HE +   RDE+  N +  ++E R +  A +   KE S  S +S   F  +
Sbjct: 481  KGTSQDVLENHEKYD--RDEIPGNLETEASEGRRNTLATKLVGKEYSIQSSHS---FSQK 540

Query: 541  SQDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQS 600
             +D   +K  K   + GGNEL++FSD PGDASL DLF PLDK+P  +  EASTS  T   
Sbjct: 541  GED-GLRKAVKTPSSFGGNELTRFSDPPGDASLHDLFHPLDKVPEGKTNEASTSTPTANV 600

Query: 601  NMGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLV 660
            N G   V D  KNDLATKLRA IAQKQME E G +  GGDL RL+MGVLKDD ++ID LV
Sbjct: 601  NQGDSPVADGGKNDLATKLRARIAQKQMEGETGHSQDGGDLFRLMMGVLKDDVLNIDDLV 660

Query: 661  FDEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHG 720
            FDEK+  ENLFPLQAVEF RL  SLRPDE ED IV++  KL+A+F QRP QK V++TQ+G
Sbjct: 661  FDEKVPPENLFPLQAVEFSRLVSSLRPDESEDAIVTSSLKLVAMFRQRPGQKAVFVTQNG 720

Query: 721  LLPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFA--VPDRPREV 780
             LPL +LL++PK+R+IC+VLQLIN+IVKDN DF ENACLVGLIPLVM FA    DR RE+
Sbjct: 721  FLPLMDLLDIPKSRVICAVLQLINEIVKDNTDFLENACLVGLIPLVMSFAGFERDRSREI 780

Query: 781  RMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQ 840
            R EAAYF QQLCQSS LTLQMF++CRGIPVLV FLEADYAK+R+MVHLAIDGMWQ+FKL+
Sbjct: 781  RKEAAYFLQQLCQSSPLTLQMFISCRGIPVLVGFLEADYAKHREMVHLAIDGMWQVFKLK 840

Query: 841  RSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPT 900
            +ST RN FCRIAAK+GILLRL+NTLYSL+EATRLASI+ G    +DG   R RSGQLDP 
Sbjct: 841  KSTSRNDFCRIAAKNGILLRLVNTLYSLSEATRLASIS-GDALILDGQTPRARSGQLDPN 900

Query: 901  HPIFSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFF 960
            +PIFSQ E S  V D PD LK R+G        G  EPS A TS+SQ SD +Q D     
Sbjct: 901  NPIFSQRETSPSVIDHPDGLKTRNG--------GGEEPSHALTSNSQSSDVHQPD--ALH 960

Query: 961  TDADRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSN 1020
             D DRP+                  L  VV  AT++      ++H            ++N
Sbjct: 961  PDGDRPR------------------LSSVVADATED----VIQQH--------RISLSAN 1020

Query: 1021 RTSTDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLE 1080
            RTSTD+  KL E  +NGFP     TQ +QVRPLLSLL+KEPPSR  SGQL+Y++H++G+E
Sbjct: 1021 RTSTDKLQKLAEGASNGFPV----TQPDQVRPLLSLLEKEPPSRKISGQLDYVKHIAGIE 1080

Query: 1081 RHETIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPT 1140
            RHE+ +PLL+AS+EKK NG+ +F+MAEFA                +VS RGK+NGNLD  
Sbjct: 1081 RHESRLPLLYASDEKKTNGDLEFIMAEFA----------------EVSGRGKENGNLDTA 1140

Query: 1141 SKVSLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVS 1200
             + S K + KKV   ++ E  AST GIASQTASGVLSGSGVLNARPGS TSSGLL+H   
Sbjct: 1141 PRYSSKTMTKKV---MAIERVASTCGIASQTASGVLSGSGVLNARPGSTTSSGLLAH--- 1200

Query: 1201 TLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCI 1260
             L+ADV+ +YL KVADLLLEFA+A+TTVKSYMCSQSLL+RLFQMFNRVEPPILLKIL+C 
Sbjct: 1201 ALSADVSMDYLEKVADLLLEFARAETTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECT 1260

Query: 1261 NHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAA 1320
            NHLSTDPNCLENLQRADAIK LIPNLELKEG LV QIH EVLSALFNLCKINKRRQE AA
Sbjct: 1261 NHLSTDPNCLENLQRADAIKQLIPNLELKEGPLVYQIHHEVLSALFNLCKINKRRQEQAA 1320

Query: 1321 ENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSV 1380
            ENGIIPHLM F++SDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV
Sbjct: 1321 ENGIIPHLMLFVMSDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSV 1367

Query: 1381 TALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSR 1440
             ALDSIAVCLA D D +KVEQA LKKDA+QKLV FFQ CPE+HFVHILEPFLKIITKSS 
Sbjct: 1381 IALDSIAVCLAQDVD-QKVEQAFLKKDAIQKLVNFFQNCPERHFVHILEPFLKIITKSSS 1367

Query: 1441 INTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLI 1454
            IN TLA+NGLTPLLIARLDHQDAIARLNLLKLIKAVYE HP+PKQLIVENDLP KLQNLI
Sbjct: 1441 INKTLALNGLTPLLIARLDHQDAIARLNLLKLIKAVYEKHPKPKQLIVENDLPQKLQNLI 1367

BLAST of Cp4.1LG03g04860 vs. ExPASy Swiss-Prot
Match: A0A078CGE6 (MAP3K epsilon protein kinase 1 OS=Brassica napus OX=3708 GN=M3KE1 PE=1 SV=1)

HSP 1 Score: 1601.3 bits (4145), Expect = 0.0e+00
Identity = 931/1522 (61.17%), Postives = 1039/1522 (68.27%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            M+RQ+ ++ FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIVQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LN IM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNTIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            V VYI+Q                            GLVKLADFGVATKL EADVNTHSVV
Sbjct: 121  VTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLNEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            D  PPIPDSLSPDITDFLRQCFKK                        D+RQRPDAKTLL
Sbjct: 301  DDSPPIPDSLSPDITDFLRQCFKK------------------------DSRQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------KELSSDVVTDMS------------- 420
            SHPWI+N RRAL SSLRHSGT+R             E S D+   +S             
Sbjct: 361  SHPWIRNSRRALQSSLRHSGTIRYMKGADSSSEKDGEGSQDIAESVSAEKVGMSKTNSKS 420

Query: 421  -------KSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIATSEPIE 480
                   +S+K  +S  +  EE    EDD + DQ PTLSIH+N S L  S   + S   +
Sbjct: 421  KLGVGSFRSEKDQSSASDIGEERADSEDDIMSDQGPTLSIHDNKSSLQ-SSTCSISSDAK 480

Query: 481  FHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPESQDNSFQKVS 540
                 G++  +  +  +      +  A +Q G KE+S      S  F P+ +D   +K  
Sbjct: 481  GTSQDGKSEPDGNLEMEASEGRRKASATKQVG-KESSIQMQQRSHSFGPKGEDRGLRKAV 540

Query: 541  KMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMGYLSVNDV 600
            K   + GGNEL++FSD PGDA L DLF PL+K+P  +  EAS S     +N G   V D 
Sbjct: 541  KTPSSYGGNELTRFSDPPGDACLHDLFHPLNKVPEGKLNEASASTPASNANQGDSPVADG 600

Query: 601  VKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDEKLSGENL 660
             KNDLATKLRA IAQKQME E G ++ GGDL RL+MGVLKDD IDIDGLVFDEK S +NL
Sbjct: 601  GKNDLATKLRARIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEKASPDNL 660

Query: 661  FPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLPLTELLEV 720
             PLQAVEF RL  SLRP E ED IV++CQKL+A+F  RPEQK+V++TQHG LP+ +LL+ 
Sbjct: 661  LPLQAVEFSRLVSSLRPSETEDAIVTSCQKLVAMFRHRPEQKVVFVTQHGFLPVMDLLDS 720

Query: 721  PKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVP--DRPREVRMEAAYFFQQ 780
            PK+R+ C+VLQLIN+I+KDN+DFQENACLVGLIPLVM FA P  DR RE+R EAAYF QQ
Sbjct: 721  PKSRVTCAVLQLINEIIKDNIDFQENACLVGLIPLVMSFAGPERDRSREIRKEAAYFLQQ 780

Query: 781  LCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLRNGFCR 840
            LCQSSSLTLQMF+ACRGIPVLV FLEADYAKYR MVHLAIDGMWQ+FKL+RST RN FCR
Sbjct: 781  LCQSSSLTLQMFIACRGIPVLVGFLEADYAKYRSMVHLAIDGMWQVFKLKRSTPRNDFCR 840

Query: 841  IAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIFSQYEAS 900
            IAAK+GILLRLINTLYSLNEAT LAS                RSGQLD       Q+EA 
Sbjct: 841  IAAKNGILLRLINTLYSLNEATLLAS--------------EGRSGQLD-------QHEAL 900

Query: 901  FPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADRPQSSN 960
              V D PD+LK R G        G  EP     S+SQRSD  Q        D DRP+SS+
Sbjct: 901  LSVIDHPDVLKTRPG--------GGEEP-----SNSQRSDLYQ-------PDGDRPRSSS 960

Query: 961  TTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTSTDRPPKL 1020
                         A+LD     AT++      K+H  +         +SNRTSTD+  KL
Sbjct: 961  -------------AALD-----ATED-----VKQHHRIS-------ISSNRTSTDKIQKL 1020

Query: 1021 LEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETIMPLLH 1080
             E  +NG+    A TQ EQVRPLLSLL+KEPPSRH SGQL+Y++H++GLE+HE+I+PLL 
Sbjct: 1021 AESASNGY----AVTQPEQVRPLLSLLEKEPPSRHVSGQLDYVKHIAGLEKHESILPLLR 1080

Query: 1081 ASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKVSLKAVAK 1140
            AS                                            +D   +   K ++K
Sbjct: 1081 AS--------------------------------------------IDTMPRYFSKTMSK 1140

Query: 1141 KVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNADVAREY 1200
            KV   ++ EGAAS        ASGVLSGSGVLNAR GS TSSGLLSHMV+TL+A+VA +Y
Sbjct: 1141 KV---MAIEGAAS--------ASGVLSGSGVLNARLGSDTSSGLLSHMVTTLSAEVASQY 1200

Query: 1201 LAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLSTDPNCL 1260
            L KVADLLLEFA+ADTTVKSYMCSQSLL+RLF MFNRVEPPILLKILKC NHLSTDPNCL
Sbjct: 1201 LEKVADLLLEFARADTTVKSYMCSQSLLSRLFHMFNRVEPPILLKILKCTNHLSTDPNCL 1260

Query: 1261 ENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENGIIPHLMH 1320
            E+LQRADAIK+LIPNLE+KEG+LV QIH EVLSALFNLCKINKRRQE AAENGIIPHLM 
Sbjct: 1261 ESLQRADAIKHLIPNLEVKEGNLVDQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLML 1299

Query: 1321 FIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTALDSIAVCL 1380
            F++SDSPLKQYALPLLCDMAHASRNSREQLR+HGGLDVYLSLL+DE WSV ALDSIAVCL
Sbjct: 1321 FVMSDSPLKQYALPLLCDMAHASRNSREQLRSHGGLDVYLSLLDDEYWSVIALDSIAVCL 1299

Query: 1381 AHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGL 1440
            A DNDNRKVEQALLK DA+  LV FFQ CPE+HFVHILEPFLKIITKSSRINTTLAVNGL
Sbjct: 1381 AQDNDNRKVEQALLKDDAIYTLVNFFQSCPERHFVHILEPFLKIITKSSRINTTLAVNGL 1299

Query: 1441 TPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSG 1454
            TPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLP +LQNLIEERR+GQ  G
Sbjct: 1441 TPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPQRLQNLIEERREGQHLG 1299

BLAST of Cp4.1LG03g04860 vs. ExPASy Swiss-Prot
Match: Q8T2I8 (Serine/threonine-protein kinase sepA OS=Dictyostelium discoideum OX=44689 GN=sepA PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 1.6e-128
Identity = 433/1552 (27.90%), Postives = 656/1552 (42.27%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MS++ P     K+ T+ N Y LG  IGKG +G VY+GLD+E+GDFVAIKQ++L  I ++ 
Sbjct: 1    MSKKEP-EEIKKNVTVGN-YNLGVVIGKGGFGTVYQGLDIEDGDFVAIKQINLTKIPKDQ 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            L  IM       NLNH NIVKY+  +KTK +L+I+LEYVENGSL+ IIK  KFG FPE+L
Sbjct: 61   LQGIMNEIDLLKNLNHANIVKYIKYVKTKDNLYIVLEYVENGSLSGIIK--KFGKFPETL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            V VYI Q                            G +KLADFGVATK    D +  +VV
Sbjct: 121  VCVYIRQVLEGLVYLHEQGVVHRDIKGANILTTKEGKIKLADFGVATKFD--DTSAAAVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ----KIV 240
            GTPYWMAPE+IE++G    SDIWSVGCTVIELLT  PPYYDL  MPALFRIVQ     + 
Sbjct: 181  GTPYWMAPEIIELNGATTKSDIWSVGCTVIELLTGSPPYYDLGQMPALFRIVQDDCPPLP 240

Query: 241  IG-SVGFKQFAHLQSF--------SSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCL 300
             G S   K +  +Q F        S+  L+K  +++  I       +  PV  EN    +
Sbjct: 241  EGISPPLKDWL-MQCFQKDPNLRISAQKLLKHKWIQASI-------KKKPV--ENGAGGV 300

Query: 301  KNKQHQTLGGQLLDKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAK-RTYLR 360
             N    +LG       P   D ++ +ITD+  +  KK   +H+  P +     K + +L 
Sbjct: 301  GNGT-DSLGA------PANIDDIAKNITDYNERINKK--PSHQRKPSIHPKSPKGKVFLP 360

Query: 361  PQDARQ---------------RPDAKTLLSHPWIQNCRRALHSSLRHSGTLRKELSSDVV 420
            P +  +                PD K+ L    + N + +  + L+   T    +     
Sbjct: 361  PPEEEEDEWGDDFSNTPKSIKLPDKKSPLK---LTNNKPS--TPLKQQPTNNTPVQQQQQ 420

Query: 421  TDMSKSQKIFASGPNSVEEAESLEDD-----NLLDQVPTLSIHENSSLLTGSGRIA---- 480
                   K+       +E  +   DD     +L   V +L+ + N    T    I     
Sbjct: 421  QQQPPPLKLAVPKQPVIENDDDWGDDFNTVSDLSKAVGSLNFNNNKKNETPKPNIKKPTF 480

Query: 481  TSEPIEFHESHG---RARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            + +  E  +  G      DE    GD+P+       + +   K  S   GNSS      +
Sbjct: 481  SEDEDEDDDDDGFGSGGDDEDDDFGDIPT-------SIKLNPKFGSNIKGNSSGSANTTN 540

Query: 541  QDNSFQKVSKMSIALGGNELS-KFSDTPGDASLDDLFQPLD------KLPG---DQAAEA 600
              ++  +  K++++   N  + K   +P   S  ++ + ++      K  G   DQ  E 
Sbjct: 541  SSSTVVQQPKLTVSNNNNNNNKKLPLSPRQPSSGNVKEGINHGSTGSKSGGVIIDQWGED 600

Query: 601  ST------SLSTLQSNMGYLSVNDVVKNDLATKLRATIAQKQME-----NEMGQASSGGD 660
                     ++T+  +   +    V K DL+T+L+  IA  +       N  G      D
Sbjct: 601  GEEDNDWGDVATVNFDPKVIRKGTVNKPDLSTRLKNRIALSETALSNSFNNNGNDDEDED 660

Query: 661  LLRLVMGVLKDDDIDIDGLVFDEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQK 720
            +        +DDD D D    D+ L  +N +   + E  +L   L P++PE+VI SAC +
Sbjct: 661  I--FADDFDEDDDEDFD---LDKNLMKDN-YARMSSEILKLMNLLTPEQPEEVISSACTQ 720

Query: 721  LIAIFHQRPEQKIVYITQHGLLPLTELLEVP--KTRIICSVLQLINQIVKDNVDFQENAC 780
            LI +F +  EQK + I +HG++P+ E+LEV   ++ ++CS+L+++NQI+ +N++ QEN C
Sbjct: 721  LITMFKENSEQKTLLIRRHGVIPIMEMLEVSNIQSHVLCSILKVVNQIIDNNMEIQENLC 780

Query: 781  LVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYA 840
            LVG IP +M F+ P+ P  VR+E A F  ++C +S+LTLQMF+AC+G+P+LV FL + YA
Sbjct: 781  LVGGIPAIMKFSGPEYPASVRLETASFISKMCSTSTLTLQMFIACKGLPILVDFLLSPYA 840

Query: 841  KYRDMVHLAIDGMWQIFKLQRSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVG 900
            + + +V +A+D +  +F+LQ  T +N FCR+ +K G+L  L                   
Sbjct: 841  ESKRLVWMAVDAIVNVFELQSPTPKNDFCRLFSKCGLLKTL------------------- 900

Query: 901  AGYPVDGLPQRPRSGQLDPTHPIFSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSR 960
                                                                        
Sbjct: 901  ------------------------------------------------------------ 960

Query: 961  ASTSHSQRSDANQSDHRQFFTDADRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYAS 1020
                                                                        
Sbjct: 961  ------------------------------------------------------------ 1020

Query: 1021 ASKEHENVDRWRPERMANSNRTSTDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKE 1080
                                      P  L + +A+G                       
Sbjct: 1021 --------------------------PIVLRDSIADG----------------------- 1080

Query: 1081 PPSRHFSGQLEYMRHLSGLERHETIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIV 1140
                                                                        
Sbjct: 1081 ------------------------------------------------------------ 1140

Query: 1141 ILFIPDVSQRGKDNGNLDPTSKVSLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSG 1200
                                                                        
Sbjct: 1141 ------------------------------------------------------------ 1167

Query: 1201 VLNARPGSATSSGLLSHMVSTLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNR 1260
                                    + A  Y  ++ +L + F+ AD+ V+  M +  ++  
Sbjct: 1201 ------------------------EAAATYPDRIINLFIMFSAADSVVRKTMSAVEVIRP 1167

Query: 1261 LFQMFNRVEPPILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTE 1320
            +    +++ P  L K+LK I  LS D N L NLQ A AI++++P L  + G+ V++IH  
Sbjct: 1261 ILDTLSQLMPEQLAKVLKSIKQLSMDHNTLANLQNAGAIRFMVPFLGRRTGAFVAEIHNH 1167

Query: 1321 VLSALFNLCKINKRRQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQL 1380
            VL+ +F+LC+I+  RQ  AA +GIIPHL +FI S SPL Q+ALP++CD+AH S+ +R +L
Sbjct: 1321 VLNTMFHLCRIDPERQYQAAIDGIIPHLQYFITSHSPLNQFALPIICDLAH-SKKARSEL 1167

Query: 1381 RAHGGLDVYLSLLEDELWSVTALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCP 1440
              + G+  YLSLLE+  W V ALDS+AV +   ++  KVE  +   + ++KL++ F    
Sbjct: 1381 WKNNGVAFYLSLLEERYWQVNALDSLAVWIT--DETHKVENIIATNENIKKLIQLFTNAE 1167

Query: 1441 EQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHH 1454
             Q F  ILEP LKII  S  +N  L  +     +I +L H +   RLNLLK+I ++YE H
Sbjct: 1441 SQSFAGILEPLLKIIQISIPVNILLGTSNFITKIIDKLGHTNPQVRLNLLKIITSLYECH 1167

BLAST of Cp4.1LG03g04860 vs. ExPASy Swiss-Prot
Match: Q5B4Z3 (Cytokinesis protein sepH OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=sepH PE=3 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 3.5e-99
Identity = 397/1587 (25.02%), Postives = 593/1587 (37.37%), Query Frame = 0

Query: 20   YMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNIIM-------NLNHKNI 79
            Y LGD +G+GA+G VY+ L+   G+ VA+KQ+ L ++ + +L +IM       NL+H NI
Sbjct: 60   YQLGDCLGRGAFGSVYRALNWNTGETVAVKQIKLADLPKSELRVIMLEIDLLKNLDHPNI 119

Query: 80   VKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ------------ 139
            VKY G +K+   L+IILEY ENGSL +I K   FG FPE+LV VY+SQ            
Sbjct: 120  VKYQGFVKSAETLNIILEYCENGSLHSIAK--NFGRFPETLVGVYMSQVLHGLLYLHDQG 179

Query: 140  ----------------GLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAA 199
                            GLVKLADFGVA++ T   ++  SVVGTPYWMAPEVIE+SG   A
Sbjct: 180  VIHRDIKGANILTTKEGLVKLADFGVASRTT--GLSESSVVGTPYWMAPEVIELSGATTA 239

Query: 200  SDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQSFSSLCLVKV 259
            SDIWS+GCTVIELL   PPYY+LQPMPALFRIV                           
Sbjct: 240  SDIWSLGCTVIELLEGKPPYYNLQPMPALFRIVN-------------------------- 299

Query: 260  NFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLLDKHPPIPDSLSPDITDFLR 319
                                                     D HPP+P   SP + DFL 
Sbjct: 300  -----------------------------------------DDHPPLPQGASPAVKDFLM 359

Query: 320  QCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLLSHPWIQNCRR--------- 379
            QCF+K                        D   R  A+ LL HPWI N RR         
Sbjct: 360  QCFQK------------------------DPNLRVSARKLLKHPWIVNARRSDSVVPKKS 419

Query: 380  -----------------------ALHSSLRHSGTLRKELSSDV------VTDMSKSQKIF 439
                                   AL    R+       L  D       VT  S   +I 
Sbjct: 420  TEYEEAVKSVQEWNEALRSPESNALRRGTRNENQNPPSLRLDTRHTPTKVTLPSPVSRIV 479

Query: 440  A---SGPNSVEEAESLEDDNLLD------QVPTLSIHENSSLLTGSGRIATSEPIE---- 499
            A   S P+S EE ++ +DD          Q+P L  H+N   +  S ++     ++    
Sbjct: 480  ADKFSSPSSGEE-DNWDDDFATAISPSALQLPHLRPHDNFGGMLSSEKLKAFASLDGTVL 539

Query: 500  -----FHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSG-------------- 559
                 F ES    +  ++     P   +R   ++Q     + + +G              
Sbjct: 540  KSDESFEESDDPFKGSLLAGEHDPLKTIRPPPSQQANSGTSQSQNGPYGAHMRRGPPLNT 599

Query: 560  --NSSFGFEPESQDNSFQKVSKMSIALGGNELSKFSD--TPGDASLD---DLFQPLDKLP 619
                 +G +     +S  +  +  +    N +  +SD  +  +  LD     FQ  D+  
Sbjct: 600  AITPVYGGQMLQNSSSPIRQQRPPLFYKENSVEDYSDLISANEDVLDRKISAFQESDEHK 659

Query: 620  GDQAAEASTSLSTLQSNMGYLSVNDVVKNDLATKLRATIAQKQM-ENEMGQASSGGDLLR 679
                   S  +   QS+        + +     + R T+  +Q  ENE  +  S  D+L 
Sbjct: 660  DLADTGRSREVIRYQSSYEQEDTPRIGRQISVKRYRGTVEIQQFAENEHDEDFS--DILG 719

Query: 680  LVMGVLKDDDIDIDG-----LVFDEKLSGENLF--------PLQAVEFG----------- 739
             V GV  D     DG     L+ + KLS  +          P   +E G           
Sbjct: 720  -VDGVTLDKAESDDGSNKSTLMLNTKLSNNSWLGDLDDEDDPFALLEEGLDETDLEANIA 779

Query: 740  ------------RLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLPLTEL 799
                         L GSL+  + E+V+    ++L+A+F   PE K + I+ HG+LP+ E+
Sbjct: 780  RDKHARLRSQVEGLVGSLKTSQDEEVLGDISEQLLAVFCDFPETKNIIISAHGMLPILEI 839

Query: 800  LEVPKTR-IICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFF 859
            L++ + R I   +L+++N I+ D+ + QEN C VG IP++  FA    PRE+R+EAA F 
Sbjct: 840  LDLCRRRDITLCLLKIVNAIIYDDYEIQENLCFVGGIPIINEFAAKKYPREIRLEAAAFV 899

Query: 860  QQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLRNGF 919
            QQ+ Q+S+LTLQMFV+  G+ VLV FLE DY   RD+V + ++G+W +F+LQ ST +N F
Sbjct: 900  QQMYQTSTLTLQMFVSAGGLNVLVEFLEDDYEDERDLVLVGVNGIWSVFELQGSTPKNDF 959

Query: 920  CRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIFSQYE 979
            CRI ++S +                                       LDP   + S+  
Sbjct: 960  CRILSRSSV---------------------------------------LDPLSLVLSR-- 1019

Query: 980  ASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADRPQS 1039
                                                                        
Sbjct: 1020 ------------------------------------------------------------ 1079

Query: 1040 SNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTSTDRPP 1099
                                                                        
Sbjct: 1080 ------------------------------------------------------------ 1139

Query: 1100 KLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETIMPL 1159
                                       +LD+E                            
Sbjct: 1140 ---------------------------VLDEE---------------------------- 1199

Query: 1160 LHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKVSLKAV 1219
                      GE    +AE   G                                     
Sbjct: 1200 ----------GE----LAEVVEG------------------------------------- 1206

Query: 1220 AKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNADVAR 1279
                                                                        
Sbjct: 1260 ------------------------------------------------------------ 1206

Query: 1280 EYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLSTDPN 1339
                ++A++   F+QA+  VK  +  +++L+R+ +   R+ P   + +LK I +LS    
Sbjct: 1320 ----RIANIFFIFSQAENHVKEMVSERTVLHRVLKELKRMTPAHQITMLKFIKNLSMLST 1206

Query: 1340 CLENLQRADAIKYLIPNLELK-EGSLVSQIHTEVLSALFNLCKINKRRQEYAAENGIIPH 1399
             L++LQ ++AI  L   L    +     ++  ++L+ ++N+C++NK RQE AA NGI+P 
Sbjct: 1380 TLDSLQNSNAIDVLTDLLRSTIKRPHFREVSNQILNTIYNMCRLNKSRQEDAALNGIVPL 1206

Query: 1400 LMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTALDSIA 1451
            L   + ++ PLK++ALP+LCDMAH+ +  R +L  + GL  Y+SLL D  W VTALD+I 
Sbjct: 1440 LQKIVKTERPLKEFALPILCDMAHSGKVGRRELWRNKGLAFYISLLSDPYWQVTALDAIF 1206

BLAST of Cp4.1LG03g04860 vs. NCBI nr
Match: XP_023528746.1 (MAP3K epsilon protein kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2525 bits (6544), Expect = 0.0
Identity = 1346/1529 (88.03%), Postives = 1346/1529 (88.03%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRTTQQDGSIEAEISNGDDQNSCEGPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES
Sbjct: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN
Sbjct: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL
Sbjct: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA
Sbjct: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS
Sbjct: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE
Sbjct: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKKINGEPDFLMAEFA                DVSQRGKDNGNLDPTSKV
Sbjct: 1081 TIMPLLHASNEKKINGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1422

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1422

BLAST of Cp4.1LG03g04860 vs. NCBI nr
Match: KAG7018050.1 (MAP3K epsilon protein kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2514 bits (6517), Expect = 0.0
Identity = 1364/1658 (82.27%), Postives = 1373/1658 (82.81%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ 120
            LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ
Sbjct: 61   LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ 120

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 121  VLEGLVYLHEQGVIHRDIKGANILTTKEARMSATSVCLIPKKEKANKASSFVTSRLEVIK 180

Query: 181  ------------------------GLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVI 240
                                    GLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVI
Sbjct: 181  GFVALRYDSCPFELSVDDLGREALGLVKLADFGVATKLTEADVNTHSVVGTPYWMAPEVI 240

Query: 241  EMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQSF 300
            EMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQSF
Sbjct: 241  EMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQSF 300

Query: 301  SSLCLVKVNFLENLIADLRNGGR---------YGPVINENFVSCLKNKQHQTLGGQLLDK 360
            SSLCLVKVNFLENLIADLRNGGR         Y  +   ++ S L+ K    L   ++D+
Sbjct: 301  SSLCLVKVNFLENLIADLRNGGRTSILQFRIAYHQISQISYASALRRKYISLLVYFVVDE 360

Query: 361  HPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLLSH 420
               +                                      L  QDARQRPDAKTLLSH
Sbjct: 361  QFDV--------------------------------------LFVQDARQRPDAKTLLSH 420

Query: 421  PWIQNCRRALHSSLRHSGTLR--------------------------------------- 480
            PWIQNCRRALHSSLRHSGTLR                                       
Sbjct: 421  PWIQNCRRALHSSLRHSGTLRNTQQDGSIEAEISNGDDQNSCEGPSAEKNEVTDSDFKAD 480

Query: 481  --KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSGRI 540
              KELSSDVVTDMSKSQK FASGPNSVEEAESLEDD LLDQVPTLSIHENSSLLTGSGRI
Sbjct: 481  SRKELSSDVVTDMSKSQKNFASGPNSVEEAESLEDDTLLDQVPTLSIHENSSLLTGSGRI 540

Query: 541  ATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPESQD 600
            ATSEPI FHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPESQD
Sbjct: 541  ATSEPIGFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPESQD 600

Query: 601  NSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMG 660
            NSFQKVSKMSIA GGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMG
Sbjct: 601  NSFQKVSKMSIASGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMG 660

Query: 661  YLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDE 720
            YLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDE
Sbjct: 661  YLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDE 720

Query: 721  KLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLP 780
            KLSGENLFPLQAVEFGRLAGSLRPDE EDVIVSACQKLIAIFHQRPEQKIVYITQHGLLP
Sbjct: 721  KLSGENLFPLQAVEFGRLAGSLRPDESEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLP 780

Query: 781  LTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRMEAA 840
            LTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRMEAA
Sbjct: 781  LTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRMEAA 840

Query: 841  YFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLR 900
            YFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLR
Sbjct: 841  YFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLR 900

Query: 901  NGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIFS 960
            NGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDP+HPIFS
Sbjct: 901  NGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPSHPIFS 960

Query: 961  QYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADR 1020
            QYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADR
Sbjct: 961  QYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADR 1020

Query: 1021 PQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTSTD 1080
            PQSSNTTNEALGSKPSELASLDKVVNLATKEPY SASKEHENVDRWRPERMANSNRTSTD
Sbjct: 1021 PQSSNTTNEALGSKPSELASLDKVVNLATKEPYVSASKEHENVDRWRPERMANSNRTSTD 1080

Query: 1081 RPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETI 1140
            RPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETI
Sbjct: 1081 RPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETI 1140

Query: 1141 MPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKVSL 1200
            MPLLHASNEKKINGEPDFLMAEFA                DVSQRGKDNGNLDPTSKVSL
Sbjct: 1141 MPLLHASNEKKINGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKVSL 1200

Query: 1201 KAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD 1260
            KAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD
Sbjct: 1201 KAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD 1260

Query: 1261 VAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLST 1320
            VAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLST
Sbjct: 1261 VAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLST 1320

Query: 1321 DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTE------------------------- 1380
            DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTE                         
Sbjct: 1321 DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTELGVKIFVYKALGRKTSSWPFLFLLM 1380

Query: 1381 ----------------------------------------------VLSALFNLCKINKR 1440
                                                          VLSALFNLCKINKR
Sbjct: 1381 VDASSRLVLLLGEGSKALGFLEKGILLQGGRYIWVLGKGHSSPWEQVLSALFNLCKINKR 1440

Query: 1441 RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE 1453
            RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE
Sbjct: 1441 RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE 1500

BLAST of Cp4.1LG03g04860 vs. NCBI nr
Match: XP_022934815.1 (MAP3K epsilon protein kinase 1-like [Cucurbita moschata] >KAG6581545.1 MAP3K epsilon protein kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2513 bits (6514), Expect = 0.0
Identity = 1340/1529 (87.64%), Postives = 1342/1529 (87.77%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNTQQDGSIEAEISNGDDQNSCEGPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDVVTDMSKSQK FASGPNSVEEAESLE+D LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVVTDMSKSQKKFASGPNSVEEAESLEEDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES
Sbjct: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN
Sbjct: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MGYL VNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGYLPVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL
Sbjct: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA
Sbjct: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPY SASKEHENVDRWRPERMANSNRTS
Sbjct: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYVSASKEHENVDRWRPERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE
Sbjct: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKKINGEPDFLMAEFA                DVSQRGKDNGNLDPTSKV
Sbjct: 1081 TIMPLLHASNEKKINGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1422

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1422

BLAST of Cp4.1LG03g04860 vs. NCBI nr
Match: XP_022983492.1 (MAP3K epsilon protein kinase 1-like [Cucurbita maxima])

HSP 1 Score: 2494 bits (6465), Expect = 0.0
Identity = 1333/1529 (87.18%), Postives = 1337/1529 (87.44%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNTQLDGSIEAEISNGDDQTSCEGPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDVVTDMSKSQK FASGPNSVEEAESLEDD LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVVTDMSKSQKNFASGPNSVEEAESLEDDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKE ST SGNSSFGFEPES
Sbjct: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKEIST-SGNSSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKL GDQAAEASTSLSTLQSN
Sbjct: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLSGDQAAEASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MGYL VNDV KNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGYLPVNDVGKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL
Sbjct: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFPVPDQPDLLKVRHG+VDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA
Sbjct: 901  FSQYEASFPVPDQPDLLKVRHGMVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPY SASKEHENVDRWRPERMANSNRTS
Sbjct: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYVSASKEHENVDRWRPERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKL+EPVANGFPTALA TQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE
Sbjct: 1021 TDRPPKLVEPVANGFPTALAATQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKK+NGEPDFLMAEFA                DVSQRGKDNGNLDPTSKV
Sbjct: 1081 TIMPLLHASNEKKMNGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1421

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1421

BLAST of Cp4.1LG03g04860 vs. NCBI nr
Match: XP_038903582.1 (MAP3K epsilon protein kinase 1-like [Benincasa hispida])

HSP 1 Score: 2390 bits (6193), Expect = 0.0
Identity = 1274/1529 (83.32%), Postives = 1298/1529 (84.89%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNTQQDGSIEAEISNGDNQNSCESPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSS+V  D SKSQK FASGP+ VEE ESLE+D LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  DDSGKELSSNVANDASKSQKHFASGPHFVEEGESLEEDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            R+ TSEP EFHESHGRA DEVIMNG+VP  ELR+DA+R+HGE+ETSTTSG  SFGFEP+S
Sbjct: 481  RLTTSEPTEFHESHGRAHDEVIMNGEVPLAELRKDASRKHGERETSTTSGRRSFGFEPDS 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQK SKMS ALGG+ELSKFSDTPGDASLDDLFQPLDK PGDQAA ASTSLSTLQSN
Sbjct: 541  QDNSFQKASKMSTALGGDELSKFSDTPGDASLDDLFQPLDKQPGDQAAGASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MG + VND  KNDLATKLRATIAQKQMENEMGQAS GGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGNVPVNDGGKNDLATKLRATIAQKQMENEMGQASGGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKL GE LFPLQAVEFGRL GSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVY+TQHGL
Sbjct: 661  DEKLPGETLFPLQAVEFGRLVGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYVTQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGL QRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLTQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFP+PDQPDLLKVRHGIVDHH S+G PEPSRASTSHSQRSD NQSDHR F TD 
Sbjct: 901  FSQYEASFPMPDQPDLLKVRHGIVDHHPSSGPPEPSRASTSHSQRSDTNQSDHRHFSTDF 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSN TNEALGSKPSELASLDKV++LA+KEP   ASKEHEN DRWR ERMANSNRTS
Sbjct: 961  DRPQSSNATNEALGSKPSELASLDKVLHLASKEPSGLASKEHENADRWRSERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKL+EP +NGFPT L  TQQEQVRPLLSLLDKEPPSRHFSGQLEYMR LSGLERHE
Sbjct: 1021 TDRPPKLVEPASNGFPTTLVATQQEQVRPLLSLLDKEPPSRHFSGQLEYMRQLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKKINGEPDFLMAEFA                +VSQRGKDNGN DPTSKV
Sbjct: 1081 TIMPLLHASNEKKINGEPDFLMAEFA----------------EVSQRGKDNGNFDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLK  AKK+GP +SNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKTAAKKLGPLISNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1422

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1422

BLAST of Cp4.1LG03g04860 vs. ExPASy TrEMBL
Match: A0A6J1F8T2 (MAP3K epsilon protein kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441874 PE=4 SV=1)

HSP 1 Score: 2513 bits (6514), Expect = 0.0
Identity = 1340/1529 (87.64%), Postives = 1342/1529 (87.77%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNTQQDGSIEAEISNGDDQNSCEGPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDVVTDMSKSQK FASGPNSVEEAESLE+D LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVVTDMSKSQKKFASGPNSVEEAESLEEDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES
Sbjct: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN
Sbjct: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MGYL VNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGYLPVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL
Sbjct: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA
Sbjct: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPY SASKEHENVDRWRPERMANSNRTS
Sbjct: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYVSASKEHENVDRWRPERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE
Sbjct: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKKINGEPDFLMAEFA                DVSQRGKDNGNLDPTSKV
Sbjct: 1081 TIMPLLHASNEKKINGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1422

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1422

BLAST of Cp4.1LG03g04860 vs. ExPASy TrEMBL
Match: A0A6J1IZG1 (MAP3K epsilon protein kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482083 PE=4 SV=1)

HSP 1 Score: 2494 bits (6465), Expect = 0.0
Identity = 1333/1529 (87.18%), Postives = 1337/1529 (87.44%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNTQLDGSIEAEISNGDDQTSCEGPSAEKNEVTDSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDVVTDMSKSQK FASGPNSVEEAESLEDD LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVVTDMSKSQKNFASGPNSVEEAESLEDDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKE ST SGNSSFGFEPES
Sbjct: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKEIST-SGNSSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKL GDQAAEASTSLSTLQSN
Sbjct: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLSGDQAAEASTSLSTLQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MGYL VNDV KNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGYLPVNDVGKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL
Sbjct: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFPVPDQPDLLKVRHG+VDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA
Sbjct: 901  FSQYEASFPVPDQPDLLKVRHGMVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPY SASKEHENVDRWRPERMANSNRTS
Sbjct: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYVSASKEHENVDRWRPERMANSNRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TDRPPKL+EPVANGFPTALA TQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE
Sbjct: 1021 TDRPPKLVEPVANGFPTALAATQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            TIMPLLHASNEKK+NGEPDFLMAEFA                DVSQRGKDNGNLDPTSKV
Sbjct: 1081 TIMPLLHASNEKKMNGEPDFLMAEFA----------------DVSQRGKDNGNLDPTSKV 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
            SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN
Sbjct: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL
Sbjct: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG
Sbjct: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL
Sbjct: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT
Sbjct: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1421

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1453
            TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER
Sbjct: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1421

BLAST of Cp4.1LG03g04860 vs. ExPASy TrEMBL
Match: A0A5D3BYF8 (MAP3K epsilon protein kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1856G00210 PE=4 SV=1)

HSP 1 Score: 2377 bits (6160), Expect = 0.0
Identity = 1271/1538 (82.64%), Postives = 1296/1538 (84.27%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ 120
            LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ
Sbjct: 61   LNIIMNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ 120

Query: 121  -----------------------------------------GLVKLADFGVATKLTEADV 180
                                                     GLVKLADFGVATKLTEADV
Sbjct: 121  VLEGLVYLHEQGVIHRDIKGANILTTKEASLNFTLQSFILKGLVKLADFGVATKLTEADV 180

Query: 181  NTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQK 240
            NTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ 
Sbjct: 181  NTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ- 240

Query: 241  IVIGSVGFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQT 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  LGGQLLDKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRP 360
                  DKHPPIPDSLSPDITDFLRQCFKK                        DARQRP
Sbjct: 301  ------DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRP 360

Query: 361  DAKTLLSHPWIQNCRRALHSSLRHSGTLR------------------------------- 420
            DAKTLLSHPWIQNCRRALHSSLRHSGTLR                               
Sbjct: 361  DAKTLLSHPWIQNCRRALHSSLRHSGTLRNVQQDGSIEAEISTGDNQNSCESPSAEKNEV 420

Query: 421  ----------KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSS 480
                      KELSSDV TD SKSQK FASGPN VEE ESLE+D LLDQVPTLSIHENSS
Sbjct: 421  ADSDFKADSRKELSSDVATDASKSQKHFASGPNFVEEGESLEEDTLLDQVPTLSIHENSS 480

Query: 481  LLTGSGRIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSF 540
            LLTGSGR+ATS P EFHESHGRA DEVIMNG+VP TELR+DA+R+ GE+ETSTTSG  SF
Sbjct: 481  LLTGSGRLATSGPTEFHESHGRAHDEVIMNGEVPLTELRKDASRKQGEQETSTTSGRRSF 540

Query: 541  GFEPESQDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSL 600
            GFEPESQDNSFQKVSKMSIALGG+ELSKFSDTPGDASLDDLFQPLDK  GDQA  ASTSL
Sbjct: 541  GFEPESQDNSFQKVSKMSIALGGDELSKFSDTPGDASLDDLFQPLDKHSGDQATGASTSL 600

Query: 601  STLQSNMGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDID 660
            S LQSNMG + VNDV KNDLATKLRATIAQKQMENEMGQAS GGDLLRLVMGVLKDDDID
Sbjct: 601  SILQSNMGNVPVNDVGKNDLATKLRATIAQKQMENEMGQASGGGDLLRLVMGVLKDDDID 660

Query: 661  IDGLVFDEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVY 720
            IDGLVFDEKL GE LFPLQAVEFGRL GSLRPDEPEDVIVS CQKLIAIFHQRPEQKIVY
Sbjct: 661  IDGLVFDEKLPGETLFPLQAVEFGRLVGSLRPDEPEDVIVSTCQKLIAIFHQRPEQKIVY 720

Query: 721  ITQHGLLPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRP 780
            +TQHGLLPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVG+IPLVMGFAVPDRP
Sbjct: 721  VTQHGLLPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGMIPLVMGFAVPDRP 780

Query: 781  REVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIF 840
            REVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIF
Sbjct: 781  REVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIF 840

Query: 841  KLQRSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQL 900
            KLQRSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGL QRPRSGQL
Sbjct: 841  KLQRSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLTQRPRSGQL 900

Query: 901  DPTHPIFSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHR 960
            DP+HPIFSQYEASFP+PDQPDLLKVRHGIVDHH STG PEPSRASTSHSQRSD NQSD+R
Sbjct: 901  DPSHPIFSQYEASFPMPDQPDLLKVRHGIVDHHSSTGPPEPSRASTSHSQRSDTNQSDYR 960

Query: 961  QFFTDADRP---QSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPE 1020
             F T+ DRP   QSSN TNEALGSKPSELASLDKV++LA+KEP  SASK+HEN DRWR +
Sbjct: 961  HFPTETDRPDRPQSSNATNEALGSKPSELASLDKVLHLASKEPSGSASKDHENADRWRTD 1020

Query: 1021 RMANSNRTSTDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMR 1080
            RMANSNRTSTDRPPK +EP +NGF T L  TQQEQVRPLLSLLDKEPPSRHFSGQLEYMR
Sbjct: 1021 RMANSNRTSTDRPPKFVEPASNGFSTTLVATQQEQVRPLLSLLDKEPPSRHFSGQLEYMR 1080

Query: 1081 HLSGLERHETIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDN 1140
             LSGLERHETIMPLLHASNEKKINGEPDFLMAEFA                +VSQRGKDN
Sbjct: 1081 QLSGLERHETIMPLLHASNEKKINGEPDFLMAEFA----------------EVSQRGKDN 1140

Query: 1141 GNLDPTSKVSLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGL 1200
            GNLDP SKVSLK  AKKVGP +SNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGL
Sbjct: 1141 GNLDPASKVSLKTAAKKVGPLISNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGL 1200

Query: 1201 LSHMVSTLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILL 1260
            LSHMVSTLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEP ILL
Sbjct: 1201 LSHMVSTLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPSILL 1260

Query: 1261 KILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKR 1320
            KILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKR
Sbjct: 1261 KILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKR 1320

Query: 1321 RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE 1380
            RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE
Sbjct: 1321 RQEYAAENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLE 1380

Query: 1381 DELWSVTALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKI 1440
            D+LWSVTALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKI
Sbjct: 1381 DDLWSVTALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKI 1431

Query: 1441 ITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPH 1453
            ITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPH
Sbjct: 1441 ITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPH 1431

BLAST of Cp4.1LG03g04860 vs. ExPASy TrEMBL
Match: A0A1S3CFK1 (MAP3K epsilon protein kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103500361 PE=4 SV=1)

HSP 1 Score: 2375 bits (6155), Expect = 0.0
Identity = 1271/1532 (82.96%), Postives = 1296/1532 (84.60%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRALHSSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALHSSLRHSGTLRNVQQDGSIEAEISTGDNQNSCESPSAEKNEVADSDFK 420

Query: 421  ----KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSG 480
                KELSSDV TD SKSQK FASGPN VEE ESLE+D LLDQVPTLSIHENSSLLTGSG
Sbjct: 421  ADSRKELSSDVATDASKSQKHFASGPNFVEEGESLEEDTLLDQVPTLSIHENSSLLTGSG 480

Query: 481  RIATSEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPES 540
            R+ATS P EFHESHGRA DEVIMNG+VP TELR+DA+R+ GE+ETSTTSG  SFGFEPES
Sbjct: 481  RLATSGPTEFHESHGRAHDEVIMNGEVPLTELRKDASRKQGEQETSTTSGRRSFGFEPES 540

Query: 541  QDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSN 600
            QDNSFQKVSKMSIALGG+ELSKFSDTPGDASLDDLFQPLDK  GDQA  ASTSLS LQSN
Sbjct: 541  QDNSFQKVSKMSIALGGDELSKFSDTPGDASLDDLFQPLDKHSGDQATGASTSLSILQSN 600

Query: 601  MGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVF 660
            MG + VNDV KNDLATKLRATIAQKQMENEMGQAS GGDLLRLVMGVLKDDDIDIDGLVF
Sbjct: 601  MGNVPVNDVGKNDLATKLRATIAQKQMENEMGQASGGGDLLRLVMGVLKDDDIDIDGLVF 660

Query: 661  DEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGL 720
            DEKL GE LFPLQAVEFGRL GSLRPDEPEDVIVS CQKLIAIFHQRPEQKIVY+TQHGL
Sbjct: 661  DEKLPGETLFPLQAVEFGRLVGSLRPDEPEDVIVSTCQKLIAIFHQRPEQKIVYVTQHGL 720

Query: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRME 780
            LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVG+IPLVMGFAVPDRPREVRME
Sbjct: 721  LPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGMIPLVMGFAVPDRPREVRME 780

Query: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840
            AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST
Sbjct: 781  AAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRST 840

Query: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPI 900
            LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGL QRPRSGQLDP+HPI
Sbjct: 841  LRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLTQRPRSGQLDPSHPI 900

Query: 901  FSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
            FSQYEASFP+PDQPDLLKVRHGIVDHH STG PEPSRASTSHSQRSD NQSD+R F T+ 
Sbjct: 901  FSQYEASFPMPDQPDLLKVRHGIVDHHSSTGPPEPSRASTSHSQRSDTNQSDYRHFPTET 960

Query: 961  DRP---QSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSN 1020
            DRP   QSSN TNEALGSKPSELASLDKV++LA+KEP  SASK+HEN DRWR +RMANSN
Sbjct: 961  DRPDRPQSSNATNEALGSKPSELASLDKVLHLASKEPSGSASKDHENADRWRTDRMANSN 1020

Query: 1021 RTSTDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLE 1080
            RTSTDRPPK +EP +NGF T L  TQQEQVRPLLSLLDKEPPSRHFSGQLEYMR LSGLE
Sbjct: 1021 RTSTDRPPKFVEPASNGFSTTLVATQQEQVRPLLSLLDKEPPSRHFSGQLEYMRQLSGLE 1080

Query: 1081 RHETIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPT 1140
            RHETIMPLLHASNEKKINGEPDFLMAEFA                +VSQRGKDNGNLDP 
Sbjct: 1081 RHETIMPLLHASNEKKINGEPDFLMAEFA----------------EVSQRGKDNGNLDPA 1140

Query: 1141 SKVSLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVS 1200
            SKVSLK  AKKVGP +SNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVS
Sbjct: 1141 SKVSLKTAAKKVGPLISNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVS 1200

Query: 1201 TLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCI 1260
            TLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEP ILLKILKCI
Sbjct: 1201 TLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPSILLKILKCI 1260

Query: 1261 NHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAA 1320
            NHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAA
Sbjct: 1261 NHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAA 1320

Query: 1321 ENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSV 1380
            ENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED+LWSV
Sbjct: 1321 ENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDDLWSV 1380

Query: 1381 TALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSR 1440
            TALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSR
Sbjct: 1381 TALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSR 1425

Query: 1441 INTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLI 1453
            INTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLI
Sbjct: 1441 INTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLI 1425

BLAST of Cp4.1LG03g04860 vs. ExPASy TrEMBL
Match: A0A6J1DGN2 (MAP3K epsilon protein kinase 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111020692 PE=4 SV=1)

HSP 1 Score: 2278 bits (5903), Expect = 0.0
Identity = 1233/1527 (80.75%), Postives = 1266/1527 (82.91%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LNIIM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNIIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYISQ                            GLVKLADFGVATKLTEADVNTHSVV
Sbjct: 121  VAVYISQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLTEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            DKHPPIPDSLSPDITDFLRQCFKK                        DARQRPDAKTLL
Sbjct: 301  DKHPPIPDSLSPDITDFLRQCFKK------------------------DARQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR------------------------------------- 420
            SHPWIQNCRRAL SSLRHSGTLR                                     
Sbjct: 361  SHPWIQNCRRALQSSLRHSGTLRNIQEDGSTEAEISNGDNQNSRESSSVEKNEVADSDSR 420

Query: 421  KELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIAT 480
            KELSSDV  D SKSQK FASGPN VEE ESLE+D LLDQVPTLSIHENSSLLTGSGR+AT
Sbjct: 421  KELSSDVAIDASKSQKNFASGPNFVEERESLEEDTLLDQVPTLSIHENSSLLTGSGRLAT 480

Query: 481  SEPIEFHESHGRARDEVIMNGDVPSTELREDAARQHGEKETSTTSGNSSFGFEPESQDNS 540
            S PIEF ES  R R+EV+MN +VP  ELR+D +R+HGEKETST SG+  FGF PESQDN+
Sbjct: 481  STPIEFDESQNRVREEVVMNXEVPPPELRKDVSRKHGEKETST-SGSRPFGFGPESQDNN 540

Query: 541  FQKVSKMSIALGG-NELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMGY 600
            FQK SKMSIALGG NELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTS+ST QSN+G 
Sbjct: 541  FQKASKMSIALGGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSVSTPQSNVGN 600

Query: 601  LSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDEK 660
            + VNDV KNDLATKLRATIAQKQMENEMGQA+ GGDLLRLVMGVLKDDDIDIDGLVFDEK
Sbjct: 601  MPVNDVGKNDLATKLRATIAQKQMENEMGQANGGGDLLRLVMGVLKDDDIDIDGLVFDEK 660

Query: 661  LSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLPL 720
            L GENLFPLQAVEF RL GSLRPDEPED+IVS CQKLIA FHQRPEQKIVY+TQHGLLPL
Sbjct: 661  LPGENLFPLQAVEFSRLVGSLRPDEPEDIIVSTCQKLIAFFHQRPEQKIVYVTQHGLLPL 720

Query: 721  TELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVPDRPREVRMEAAY 780
            TELLEVPKTR+ICSVLQLINQIVKDN DFQENACLVGLIPLVMGFAVPDRPREVRMEAA 
Sbjct: 721  TELLEVPKTRVICSVLQLINQIVKDNSDFQENACLVGLIPLVMGFAVPDRPREVRMEAAS 780

Query: 781  FFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTLRN 840
            F QQLCQSSSLTLQMFVACRGIPVLV FLEADYAKYR+MVHLAIDGMWQIFKLQRST RN
Sbjct: 781  FLQQLCQSSSLTLQMFVACRGIPVLVGFLEADYAKYREMVHLAIDGMWQIFKLQRSTPRN 840

Query: 841  GFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIFSQ 900
             FCRIAAK+GILLRLINTLYSLNEATRLASITVGAGYPVDGL QRPRSGQLD +HPIF+Q
Sbjct: 841  DFCRIAAKNGILLRLINTLYSLNEATRLASITVGAGYPVDGLTQRPRSGQLDSSHPIFTQ 900

Query: 901  YEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDADRP 960
            YEASFP PDQPD+LKVRHGIVDHH STGTPEPSRASTSHSQRSDANQSDHR F  DADRP
Sbjct: 901  YEASFPAPDQPDILKVRHGIVDHHPSTGTPEPSRASTSHSQRSDANQSDHRHFSMDADRP 960

Query: 961  QSSNTTNEALGS-KPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTSTD 1020
            QSSNT NEALG+ K S+L SL+KVVNLATK+P                     SNRTSTD
Sbjct: 961  QSSNTLNEALGTPKSSDLVSLEKVVNLATKDP---------------------SNRTSTD 1020

Query: 1021 RPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHETI 1080
            R PKL+EPV+NGFPT+LA TQQEQVRPLLSLLDKEPPSRH SGQLEYMRHLSGLERHETI
Sbjct: 1021 RAPKLVEPVSNGFPTSLA-TQQEQVRPLLSLLDKEPPSRHLSGQLEYMRHLSGLERHETI 1080

Query: 1081 MPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKVSL 1140
            MPLLHASNEKK N EPDFLMAEFA                DVSQRGKDN NLD TSKVS 
Sbjct: 1081 MPLLHASNEKKTNSEPDFLMAEFA----------------DVSQRGKDNANLDSTSKVSH 1140

Query: 1141 KAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD 1200
            K V KKVGPQV NEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD
Sbjct: 1141 KTVTKKVGPQVLNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLNAD 1200

Query: 1201 VAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHLST 1260
            VA+EYLAKVADLLLEFAQADTTVKSYMCSQSLL+RLFQMFNRVEPPILLKILKC+NHLST
Sbjct: 1201 VAKEYLAKVADLLLEFAQADTTVKSYMCSQSLLSRLFQMFNRVEPPILLKILKCVNHLST 1260

Query: 1261 DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENGII 1320
            DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQE+AAENGII
Sbjct: 1261 DPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEHAAENGII 1320

Query: 1321 PHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTALDS 1380
            PHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTALDS
Sbjct: 1321 PHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTALDS 1380

Query: 1381 IAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTL 1440
            IAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTL
Sbjct: 1381 IAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTL 1397

Query: 1441 AVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRD 1453
            AVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRD
Sbjct: 1441 AVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRD 1397

BLAST of Cp4.1LG03g04860 vs. TAIR 10
Match: AT3G13530.1 (mitogen-activated protein kinase kinase kinase 7 )

HSP 1 Score: 1756.9 bits (4549), Expect = 0.0e+00
Identity = 991/1529 (64.81%), Postives = 1106/1529 (72.33%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            M+RQ+ ++ FHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LN IM       NLNHKNIVKYLGS KTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNTIMQEIDLLKNLNHKNIVKYLGSSKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            VAVYI+Q                            GLVKLADFGVATKL EADVNTHSVV
Sbjct: 121  VAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLNEADVNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ       
Sbjct: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            D +PPIPDSLSPDITDFLRQCFKK                        D+RQRPDAKTLL
Sbjct: 301  DDNPPIPDSLSPDITDFLRQCFKK------------------------DSRQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR--KELSSDVVTDMSKSQKI--FASGPN---------- 420
            SHPWI+N RRAL SSLRHSGT++  KE ++    D   SQ      SG N          
Sbjct: 361  SHPWIRNSRRALQSSLRHSGTIKYMKEATASSEKDDEGSQDAAESLSGENVGISKTDSKS 420

Query: 421  ----------------------SVEEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIATS 480
                                    E  ++ EDD + DQVPTLSIHE SS   G+      
Sbjct: 421  KLPLVGVSSFRSEKDQSTPSDLGEEGTDNSEDDIMSDQVPTLSIHEKSSDAKGT------ 480

Query: 481  EPIEFHESHGRA-RDEVIMNGDVPSTELRED-AARQHGEKETSTTSGNSSFGFEPESQDN 540
             P +  + HG++ R E   N    ++E R++ +A +H  KE S     +S  F  + ++ 
Sbjct: 481  -PQDVSDFHGKSERGETPENLVTETSEARKNTSAIKHVGKELSIPVDQTSHSFGRKGEER 540

Query: 541  SFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQSNMGY 600
              +K  K   ++ GNEL++FSD PGDASL DLF PLDK+   +  EASTS+ T   N G 
Sbjct: 541  GIRKAVKTPSSVSGNELARFSDPPGDASLHDLFHPLDKVSEGKPNEASTSMPTSNVNQGD 600

Query: 601  LSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLVFDEK 660
              V D  KNDLATKLRATIAQKQME E G ++ GGDL RL+MGVLKDD IDIDGLVFDEK
Sbjct: 601  SPVADGGKNDLATKLRATIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEK 660

Query: 661  LSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHGLLPL 720
            +  ENLFPLQAVEF RL  SLRPDE ED IVS+CQKL+A+F QRPEQK+V++TQHG LPL
Sbjct: 661  VPAENLFPLQAVEFSRLVSSLRPDESEDAIVSSCQKLVAMFRQRPEQKVVFVTQHGFLPL 720

Query: 721  TELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFAVP--DRPREVRMEA 780
             +LL++PK+R+IC+VLQLIN+I+KDN DFQENACLVGLIP+VM FA P  DR RE+R EA
Sbjct: 721  MDLLDIPKSRVICAVLQLINEIIKDNTDFQENACLVGLIPVVMSFAGPERDRSREIRKEA 780

Query: 781  AYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQRSTL 840
            AYF QQLCQSS LTLQMF+ACRGIPVLV FLEADYAKYR+MVHLAIDGMWQ+FKL+RST 
Sbjct: 781  AYFLQQLCQSSPLTLQMFIACRGIPVLVGFLEADYAKYREMVHLAIDGMWQVFKLKRSTP 840

Query: 841  RNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPTHPIF 900
            RN FCRIAAK+GILLRLINTLYSLNEATRLASI+ G    +DG   R RSGQLDP +PIF
Sbjct: 841  RNDFCRIAAKNGILLRLINTLYSLNEATRLASISGG----LDGQAPRVRSGQLDPNNPIF 900

Query: 901  SQYE-ASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFFTDA 960
             Q E +S  + DQPD+LK RHG        G  EPS ASTS+SQRSD +Q D      D 
Sbjct: 901  GQNETSSLSMIDQPDVLKTRHG--------GGEEPSHASTSNSQRSDVHQPD--ALHPDG 960

Query: 961  DRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSNRTS 1020
            D+P+ S+   +A  S   ++    ++                            ++NRTS
Sbjct: 961  DKPRVSSVAPDASTSGTEDVRQQHRI--------------------------SLSANRTS 1020

Query: 1021 TDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLERHE 1080
            TD+  KL E  +NGFP     TQ EQVRPLLSLLDKEPPSRH+SGQL+Y++H++G+ERHE
Sbjct: 1021 TDKLQKLAEGASNGFPV----TQTEQVRPLLSLLDKEPPSRHYSGQLDYVKHITGIERHE 1080

Query: 1081 TIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPTSKV 1140
            + +PLLH SNEKK NG+ DFLMAEFA                +VS RGK+NG+LD T++ 
Sbjct: 1081 SRLPLLHGSNEKKNNGDLDFLMAEFA----------------EVSGRGKENGSLDTTTRY 1140

Query: 1141 SLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVSTLN 1200
              K + KKV   ++ EG ASTSGIASQTASGVLSGSGVLNARPGSATSSGLL+HMVSTL+
Sbjct: 1141 PSKTMTKKV---LAIEGVASTSGIASQTASGVLSGSGVLNARPGSATSSGLLAHMVSTLS 1200

Query: 1201 ADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCINHL 1260
            ADVAREYL KVADLLLEFA+ADTTVKSYMCSQSLL+RLFQMFNRVEPPILLKIL+C NHL
Sbjct: 1201 ADVAREYLEKVADLLLEFARADTTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHL 1260

Query: 1261 STDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAAENG 1320
            STDPNCLENLQRADAIK+LIPNLELK+G LV QIH EVLSALFNLCKINKRRQE AAENG
Sbjct: 1261 STDPNCLENLQRADAIKHLIPNLELKDGHLVYQIHHEVLSALFNLCKINKRRQEQAAENG 1320

Query: 1321 IIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSVTAL 1380
            IIPHLM FI+SDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV AL
Sbjct: 1321 IIPHLMLFIMSDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIAL 1368

Query: 1381 DSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSRINT 1440
            DSIAVCLA DNDNRKVEQALLK+DA+QKLV FFQ CPE+HFVHILEPFLKIITKS RIN 
Sbjct: 1381 DSIAVCLAQDNDNRKVEQALLKQDAIQKLVDFFQSCPERHFVHILEPFLKIITKSYRINK 1368

Query: 1441 TLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEER 1454
            TLAVNGLTPLLI+RLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLP KLQNLIEER
Sbjct: 1441 TLAVNGLTPLLISRLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPQKLQNLIEER 1368

BLAST of Cp4.1LG03g04860 vs. TAIR 10
Match: AT3G07980.1 (mitogen-activated protein kinase kinase kinase 6 )

HSP 1 Score: 1653.3 bits (4280), Expect = 0.0e+00
Identity = 956/1532 (62.40%), Postives = 1078/1532 (70.37%), Query Frame = 0

Query: 1    MSRQVPTTAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60
            M+RQ+ ++ FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIGQED 60

Query: 61   LNIIM-------NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120
            LN IM       NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL
Sbjct: 61   LNTIMQEIDLLKNLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESL 120

Query: 121  VAVYISQ----------------------------GLVKLADFGVATKLTEADVNTHSVV 180
            V VYI+Q                            GLVKLADFGVATKL EAD NTHSVV
Sbjct: 121  VTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKEGLVKLADFGVATKLNEADFNTHSVV 180

Query: 181  GTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSV 240
            GTPYWMAPEVIE+SGVCAASDIWSVGCT+IELLTCVPPYYDLQPMPAL+RIVQ       
Sbjct: 181  GTPYWMAPEVIELSGVCAASDIWSVGCTIIELLTCVPPYYDLQPMPALYRIVQ------- 240

Query: 241  GFKQFAHLQSFSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLL 300
                                                                        
Sbjct: 241  ------------------------------------------------------------ 300

Query: 301  DKHPPIPDSLSPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLL 360
            D  PPIPDSLSPDITDFLR CFKK                        D+RQRPDAKTLL
Sbjct: 301  DDTPPIPDSLSPDITDFLRLCFKK------------------------DSRQRPDAKTLL 360

Query: 361  SHPWIQNCRRALHSSLRHSGTLR--KELSSDVVTDMSKSQKIF----------------- 420
            SHPWI+N RRAL SSLRHSGT+R  KE  S    D   SQ++                  
Sbjct: 361  SHPWIRNSRRALRSSLRHSGTIRYMKETDSSSEKDAEGSQEVVESVSAEKVEVTKTNSKS 420

Query: 421  ---------------ASGPNSV-EEAESLEDDNLLDQVPTLSIHENSSLLTGSGRIA--- 480
                            S P+ + EE    EDD   DQ PTLS+H+ SS  +G+  I+   
Sbjct: 421  KLPVIGGASFRSEKDQSSPSDLGEEGTDSEDDINSDQGPTLSMHDKSSRQSGTCSISSDA 480

Query: 481  ---TSEPIEFHESHGRARDEVIMNGDVPSTELREDA-ARQHGEKETSTTSGNSSFGFEPE 540
               + + +E HE +   RDE+  N +  ++E R +  A +   KE S  S +S   F  +
Sbjct: 481  KGTSQDVLENHEKYD--RDEIPGNLETEASEGRRNTLATKLVGKEYSIQSSHS---FSQK 540

Query: 541  SQDNSFQKVSKMSIALGGNELSKFSDTPGDASLDDLFQPLDKLPGDQAAEASTSLSTLQS 600
             +D   +K  K   + GGNEL++FSD PGDASL DLF PLDK+P  +  EASTS  T   
Sbjct: 541  GED-GLRKAVKTPSSFGGNELTRFSDPPGDASLHDLFHPLDKVPEGKTNEASTSTPTANV 600

Query: 601  NMGYLSVNDVVKNDLATKLRATIAQKQMENEMGQASSGGDLLRLVMGVLKDDDIDIDGLV 660
            N G   V D  KNDLATKLRA IAQKQME E G +  GGDL RL+MGVLKDD ++ID LV
Sbjct: 601  NQGDSPVADGGKNDLATKLRARIAQKQMEGETGHSQDGGDLFRLMMGVLKDDVLNIDDLV 660

Query: 661  FDEKLSGENLFPLQAVEFGRLAGSLRPDEPEDVIVSACQKLIAIFHQRPEQKIVYITQHG 720
            FDEK+  ENLFPLQAVEF RL  SLRPDE ED IV++  KL+A+F QRP QK V++TQ+G
Sbjct: 661  FDEKVPPENLFPLQAVEFSRLVSSLRPDESEDAIVTSSLKLVAMFRQRPGQKAVFVTQNG 720

Query: 721  LLPLTELLEVPKTRIICSVLQLINQIVKDNVDFQENACLVGLIPLVMGFA--VPDRPREV 780
             LPL +LL++PK+R+IC+VLQLIN+IVKDN DF ENACLVGLIPLVM FA    DR RE+
Sbjct: 721  FLPLMDLLDIPKSRVICAVLQLINEIVKDNTDFLENACLVGLIPLVMSFAGFERDRSREI 780

Query: 781  RMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYRDMVHLAIDGMWQIFKLQ 840
            R EAAYF QQLCQSS LTLQMF++CRGIPVLV FLEADYAK+R+MVHLAIDGMWQ+FKL+
Sbjct: 781  RKEAAYFLQQLCQSSPLTLQMFISCRGIPVLVGFLEADYAKHREMVHLAIDGMWQVFKLK 840

Query: 841  RSTLRNGFCRIAAKSGILLRLINTLYSLNEATRLASITVGAGYPVDGLPQRPRSGQLDPT 900
            +ST RN FCRIAAK+GILLRL+NTLYSL+EATRLASI+ G    +DG   R RSGQLDP 
Sbjct: 841  KSTSRNDFCRIAAKNGILLRLVNTLYSLSEATRLASIS-GDALILDGQTPRARSGQLDPN 900

Query: 901  HPIFSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPSRASTSHSQRSDANQSDHRQFF 960
            +PIFSQ E S  V D PD LK R+G        G  EPS A TS+SQ SD +Q D     
Sbjct: 901  NPIFSQRETSPSVIDHPDGLKTRNG--------GGEEPSHALTSNSQSSDVHQPD--ALH 960

Query: 961  TDADRPQSSNTTNEALGSKPSELASLDKVVNLATKEPYASASKEHENVDRWRPERMANSN 1020
             D DRP+                  L  VV  AT++      ++H            ++N
Sbjct: 961  PDGDRPR------------------LSSVVADATED----VIQQH--------RISLSAN 1020

Query: 1021 RTSTDRPPKLLEPVANGFPTALAGTQQEQVRPLLSLLDKEPPSRHFSGQLEYMRHLSGLE 1080
            RTSTD+  KL E  +NGFP     TQ +QVRPLLSLL+KEPPSR  SGQL+Y++H++G+E
Sbjct: 1021 RTSTDKLQKLAEGASNGFPV----TQPDQVRPLLSLLEKEPPSRKISGQLDYVKHIAGIE 1080

Query: 1081 RHETIMPLLHASNEKKINGEPDFLMAEFAAGEYLSKDLIVILFIPDVSQRGKDNGNLDPT 1140
            RHE+ +PLL+AS+EKK NG+ +F+MAEFA                +VS RGK+NGNLD  
Sbjct: 1081 RHESRLPLLYASDEKKTNGDLEFIMAEFA----------------EVSGRGKENGNLDTA 1140

Query: 1141 SKVSLKAVAKKVGPQVSNEGAASTSGIASQTASGVLSGSGVLNARPGSATSSGLLSHMVS 1200
             + S K + KKV   ++ E  AST GIASQTASGVLSGSGVLNARPGS TSSGLL+H   
Sbjct: 1141 PRYSSKTMTKKV---MAIERVASTCGIASQTASGVLSGSGVLNARPGSTTSSGLLAH--- 1200

Query: 1201 TLNADVAREYLAKVADLLLEFAQADTTVKSYMCSQSLLNRLFQMFNRVEPPILLKILKCI 1260
             L+ADV+ +YL KVADLLLEFA+A+TTVKSYMCSQSLL+RLFQMFNRVEPPILLKIL+C 
Sbjct: 1201 ALSADVSMDYLEKVADLLLEFARAETTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECT 1260

Query: 1261 NHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSALFNLCKINKRRQEYAA 1320
            NHLSTDPNCLENLQRADAIK LIPNLELKEG LV QIH EVLSALFNLCKINKRRQE AA
Sbjct: 1261 NHLSTDPNCLENLQRADAIKQLIPNLELKEGPLVYQIHHEVLSALFNLCKINKRRQEQAA 1320

Query: 1321 ENGIIPHLMHFIISDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDELWSV 1380
            ENGIIPHLM F++SDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV
Sbjct: 1321 ENGIIPHLMLFVMSDSPLKQYALPLLCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSV 1367

Query: 1381 TALDSIAVCLAHDNDNRKVEQALLKKDAVQKLVKFFQCCPEQHFVHILEPFLKIITKSSR 1440
             ALDSIAVCLA D D +KVEQA LKKDA+QKLV FFQ CPE+HFVHILEPFLKIITKSS 
Sbjct: 1381 IALDSIAVCLAQDVD-QKVEQAFLKKDAIQKLVNFFQNCPERHFVHILEPFLKIITKSSS 1367

Query: 1441 INTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLI 1454
            IN TLA+NGLTPLLIARLDHQDAIARLNLLKLIKAVYE HP+PKQLIVENDLP KLQNLI
Sbjct: 1441 INKTLALNGLTPLLIARLDHQDAIARLNLLKLIKAVYEKHPKPKQLIVENDLPQKLQNLI 1367

BLAST of Cp4.1LG03g04860 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 145.2 bits (365), Expect = 4.1e-34
Identity = 111/371 (29.92%), Postives = 168/371 (45.28%), Query Frame = 0

Query: 19  KYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIA-------------QEDLNIIM 78
           ++  G+ IG GA+GRVY G++L++G+ +AIKQV +   +             +E++ ++ 
Sbjct: 67  RWRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLK 126

Query: 79  NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ----- 138
           NL+H NIV+YLG+++    L+I++E+V  GS++++++  KFG FPE ++ +Y  Q     
Sbjct: 127 NLSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLE--KFGSFPEPVIIMYTKQLLLGL 186

Query: 139 -----------------------GLVKLADFGVATKLTE-ADVN-THSVVGTPYWMAPEV 198
                                  G ++LADFG + K+ E A VN   S+ GTPYWMAPEV
Sbjct: 187 EYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAPEV 246

Query: 199 IEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQS 258
           I  +G   ++DIWSVGCTVIE+ T  PP+                   S  ++QFA +  
Sbjct: 247 ILQTGHSFSADIWSVGCTVIEMATGKPPW-------------------SEQYQQFAAVLH 306

Query: 259 FSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLLDKHPPIPDSL 318
                                 GR                            HPPIP+ L
Sbjct: 307 I---------------------GR-------------------------TKAHPPIPEDL 346

Query: 319 SPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLLSHPWIQNCRR 347
           SP+  DFL +C       HKE  L                  R  A  LL HP++   R+
Sbjct: 367 SPEAKDFLMKCL------HKEPSL------------------RLSATELLQHPFVTGKRQ 346

BLAST of Cp4.1LG03g04860 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 139.4 bits (350), Expect = 2.2e-32
Identity = 80/205 (39.02%), Postives = 121/205 (59.02%), Query Frame = 0

Query: 23  GDEIGKGAYGRVYKGLDLENGDFVAIKQVSL-ENIA------------QEDLNIIMNLNH 82
           G  IG+GA+G VY G++L++G+ +A+KQV +  N A            +E++ ++ NL+H
Sbjct: 72  GQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKNLSH 131

Query: 83  KNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYISQ--------- 142
            NIV+YLG+++    L+I+LE+V  GS++++++  KFGPFPES+V  Y  Q         
Sbjct: 132 PNIVRYLGTVREDDTLNILLEFVPGGSISSLLE--KFGPFPESVVRTYTRQLLLGLEYLH 191

Query: 143 -------------------GLVKLADFGVATKLTEADVNT--HSVVGTPYWMAPEVIEMS 185
                              G +KLADFG + ++ E    T   S+ GTPYWMAPEVI  +
Sbjct: 192 NHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQT 251

BLAST of Cp4.1LG03g04860 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 139.0 bits (349), Expect = 2.9e-32
Identity = 109/411 (26.52%), Postives = 175/411 (42.58%), Query Frame = 0

Query: 19  KYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSL-ENIA------------QEDLNIIM 78
           ++  G  IG+GA+G VY G++L++G+ +A+KQV +  N A            +E++ ++ 
Sbjct: 22  RWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLLK 81

Query: 79  NLNHKNIVKYLGSLKTKTHLHIILEYVENGSLANIIKPNKFGPFPESLVAVYI------- 138
           NL+H NIV+YLG+++    L+I+LE+V  GS++++++  KFG FPES+V  Y        
Sbjct: 82  NLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLE--KFGAFPESVVRTYTNQLLLGL 141

Query: 139 ---------------------SQGLVKLADFGVATKLTEADV--NTHSVVGTPYWMAPEV 198
                                +QG +KLADFG + ++ E        S+ GTPYWMAPEV
Sbjct: 142 EYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPEV 201

Query: 199 IEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQKIVIGSVGFKQFAHLQS 258
           I  +G   ++DIWSVGCTVIE++T   P+                   S  +K+ A +  
Sbjct: 202 ILQTGHSFSADIWSVGCTVIEMVTGKAPW-------------------SQQYKEIAAIFH 261

Query: 259 FSSLCLVKVNFLENLIADLRNGGRYGPVINENFVSCLKNKQHQTLGGQLLDKHPPIPDSL 318
             +                                                 HPPIPD++
Sbjct: 262 IGT----------------------------------------------TKSHPPIPDNI 321

Query: 319 SPDITDFLRQCFKKACDNHKECPLLSINKAKRTYLRPQDARQRPDAKTLLSHPWIQNCRR 378
           S D  DFL +C +                        Q+   RP A  LL HP++     
Sbjct: 322 SSDANDFLLKCLQ------------------------QEPNLRPTASELLKHPFV----- 328

Query: 379 ALHSSLRHSGTLRKELSSDVVTDMSKSQKIFASGPNSVEEAESLEDDNLLD 387
                   +G  ++  S D+ + M  S     S   ++   ++   D++ D
Sbjct: 382 --------TGKQKESASKDLTSFMDNSCSPLPSELTNITSYQTSTSDDVGD 328