Cp4.1LG03g02580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRHOMBOID-like protein
LocationCp4.1LG03: 573844 .. 578791 (+)
RNA-Seq ExpressionCp4.1LG03g02580
SyntenyCp4.1LG03g02580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTCCGCGCGGTTCGGAGAAACGGGACAAGGACTTGGAGAGAATTGAATCAAACCGAGGCTTATTTTGATCTCAATCGTCATCTTCGACCGTCGATCAAGAACCAACTACTCTTGTCTTCGTTCTCTGTAGCGGAGCCCTAGTTGCCATCGTCGATTAAGGTGCGACGCTTCTTTCCTTATCGCTGCTGCTCCATTCTTTCTTATTATTTCCTGGAAGCGAACTATTTTCAGTTTGTAGTGGTTGCGTCGATGATTGTTTGATTTCTTGTTCAATTGTCAATTCACAAGTATCTGCGGCATCGATTAAAACTAGTTTACTCTCGGTTTGCTTGATTGTTCTAGTAGTTGCATCAGTGAGATTGCCTGGTTTATTTTGTTCAAATACAAATGATAGTGTCTGCAGCGTCGAGCAAGGCTAGTTTACTCTCAGCTTGTTTGACTTTGAGGTAAACTGGAAGAGACGAAGATATAGAGCTTCCTAATTCGCTTTGTGAAGCAATATTTCTTACTATGTTTATTAAGTCGAAACCAGAACTTTAGTCTAGAAGTTGTTGCTGTAGCTTGATGATCTTGTGCAAACGATGAATGTGATGTTTCCGGCTCTCTTCCCACTTTTGTATTCGAAATTTTTTTCATTCTCTTTGTACTATACTCACTTTTCTTTCAAAAGAATTTGGCGTTTTGCATGGCTCTTGCTCTAGAAGCTTGATTCTCTTTGTATTTTTGTAGTCCAGAAGATTAGGATTTATCTATGTGTTCTTGTAGGTTACTTGCAGATTTTTCTGCATGACAAACGCTTGATAGCTTGCAACCATGCATTTTATAGTTGTTAGTTTAGCACTCAATAAGAATTGTCAAATTGTTGATGCACGGAGGCCTGAATCTGAACGTTGCTTCTGTACCTTAATCTTGAATTGCTAAGAACTGCAGTATGAGAAAGTAAAATTTTCTGTTATTTTTTAAGTTTCTTTGGGCCAGATTGGTGAGAGTTTGAATTCGGCTTCAAATGCGAATGGTTCTCTTAGGATAGAAAATGCTAGTGTTTATTTATGAAGTTTACATGCTGTCATTTCTAGGCAAAACTTGTAGACGGATAATTTCAGTCAATGGAAACAGAACGGAGACAATTGACAGTTTGTACTGTAAAGGCTCACTTGGCATGTTTGCGTTGGTTTCAGTTTATATTCATCCAACCTGAGTTCATGACAAGATGTCAGGCACTGGGGAAAGATATCTCCAAGACGACGCCGATGAAGCAGCTGATGAAAATGGCAAGCAGTCCAGCGCACAAGAGTGGACGGTAGCTATCGCTGCAAAAGGTGAGGAAGGCGTCATGACTGGTTCATCAAACGCAATCCATTGCTCTATTGTCATGAGCATTGGGAAGATTTGCAGGTGCTCTTTTTAGTATCCAAAGAGAGTTAACAAAGCATATATTGTGAAGGGCCTCTGTGAAAAGTCAGGAAGGATGGCTGTTTGACGACCTGACTTTTTCGAAGGTCGGTGGGACATGGGGGCCTCACCTCATGCATCCGTTTAAAGTAGAGGAAGTATATACAGAATTAGCAAAAGTTGAGGTGAGTGATATAAACATTTTATTGGCTGCTTATTCGCTTATCATGTTGGATTACAGAGTCAAGAATGCCCTCTGGGTTGCCGCCCAAAATGCCCAAGCAGTGGCCTTCTTAGAGAATGTCCAATTAAAAACAACAATGATCATAAAATCATTCAAAATAAAAAACGCAAATTAAGCTTAAGCATACAGATTTATAGTCTTTCCACAGGCCGATTGGACTTTGGACCATTGAAATCATAAAACCCTGATTGCCGAAGCCCAGTCTCGGATCCAGACTCTTTGTTTTGTTTCAACTCATCTAGTTTAGATGACATCTTTTGTATTGGGAATTGATTTTTTCAGCCAACTTTAGACAAATAATAACCAGCTTCAGTAGACCCTTCTAGCACTAATTTATTTTTTTATGGTTTATTATTTTATGAAACAAATAACAACCATCTTTGAATTAATTATTTTATGTGTATTACTGACTTTGATTCTTCTCATTCAGAGTAGGTCAACCAATTTATTTGAAGATTCTGATCGTTGAATTTCTTAAACTGGAGTTGCTAGCTCAACTGCAACCGCCTGATCTGAGGTGTCCCCTTGTTGCAACTAATCATACAGCCAAGAATTTACAGATGCATAATAGCAATTGAAGTATTGAAGAGTACAGTATGTAAACATTATACGGCATGTTATATTTAATATTATTTTGACAATTTTGTTTATGTTTGAAATGAATAGCTATTCTTATAGTTGTAAACATGTGAAAAGTTCATTTCTTGTTTGATTGATTGGAAAAGGAGACGAAAAAACGAAGTTAATTGGGTTCTGAATTTTCTTCCTCTTTGGGATTTGATAATTGGCCCCTGCAAAATGGACTATTGCTGTATGATAACTTGAGCAACCAAAGAAAGAAGATCCTTCGCCTGAGAGACTTGTAACACGAGCCATCTCTCGTTATGAACAATTTGGCGTCTCTCATGTATCTCAAGCCGCCATGGTAGGTGGCCTTTGATTCTTGCACCTTCAAATTATGACTTGGTAAGCCTCTATCATTGTCGACCATTTTCACTTTCTTAAGTAAACTTTTTATTTAATTAATATATAATATATATACTTTTTTTTTCTTCTTTCTTTCGTCGTTTTTTATTTTCTTTTGAGTTGTTGATCAAGTTCATCCCATCGTTTCATCCTATTTTGTCTCTCTAATTTGAGAAGAGCTTCAATGGCGGAACATCACCCTTCGGATATTCAAATCATGGTCAATTCCCGGCAAGGTACTGCAATACATCCTGTAGAGGTGGATGCCCAGTCGTTGCAAGCGTCGGGTTCAATTGCTTACAGGGAAGTCAAGCAATTCAAGAAATGGATCTCTTGGTTAATTCCGTCATTTGTTATAGCCAATATTGTTATGTTCATTATCACCATGTATGTTAATAATTGCCCTAAGAATTCAGTCACTTGTATTGCTCGATTCTTGGGTAGATTCTCTTTCCAGCCCCTAAAAGAAAATCCTCTGCTTGGACCTTCATCTTTAACGTAAGTTGGTTTCACTGCATTCTCATAGTTTTTTTCTTCCGGAATTCTGTTTGTGTCAATTACATCTTGAACTTTTCGTCGTTCAAGTGAACTATGCATTGTCCACTGAACCAATTATTGCTTGTGTTTCAGGCTACGGAGTATGGGTGCATTAGAAGTTAATAAAGTGGTTCATGGAGACCAACTGTGGCGTCTCGTCACGTGTAATTGGTTACATGCCGGTGTTTTTCATTTATTGGCGAATATGTTGAGTCTCTTAGTCATTGGGATTCGTCTTGAACAAGAATTTGGATTTAGTACGTACCTTTGATCATTCTTTCCTTTTCTTTTCTGGTGTTGGTAGCAGAATTATTCCCTGAAATGCCTACCTATTTAAGAGGCGACCTTCGATTGTTGGCCACTTTATCAAGAGTTCTGTTTATTGTTTTCGTAGACATGGATTATTCTATGTTTATATATAGTGGATTCACTGGCCTTCCACCCTTAACCAACTCTTTTGTGAATGTCTTACAGTACGGATCGGCTTGCTATATGTTATATCGGGATTTGGTGGGAGTCTGTTGTCTGCTCTGTTCATCCAGTCAAACATCTCTGTCGGGGCTTCTGGTGCCTTGTTTGGGTTGCTCGGAGGAATGCTTTCTGAACTCATCACTAACTGGACGATATATTCTAATAAGGTACTCTTCAAGCCGCATTTGTATTGAAATTTTCTGATGAATCCTGACACTTAGCTTGGTTGCATTTTCTGGTCTCTTTTCCCCTTCAGGTACCAGCTCTTCTTACTCTGTTGGTCATTATAGCGATCAACTTGGCAGTAGGAATTCTTCCTCATGTTGATAATTTTGCTCATATTGGAGGATTTATTTCTGGATTTCTTCTGGGGTTTGTATTTCTCATTCGCCCGCAGTTTGGATGGGTTAGCCGAAGATATACTTCTATAACAAATTCTTCCATCCCCGTCGAACATAAATTCAAGATATATCAATGCGTATTATGGATCATTTCGCTAATACTTCTGATTGCCGGGTAAGCAATTCACTCTATATTTGTGCTGCCTTTATCCAATTGTATTTAGAGAGCATACAAAATTAGAACTGGAGTCACAAGGTATAGGAGATTGTTTTTTTTTTATTTTTAACCCAAGATATCTCAAGAAACCATGAACTTGATGTTACAAAATCTTGTTTTGATGGTTAAACAATATTTCAGCGTCCCATGGAACTTCTTTAGTTATAAAAAAACGATAATAATCTTCAGAAAGATGCATATTTTCGCAATTAGGGAAGGAATGATCTTGGAAAAGTTGTTAGTTTACTCCACGCTGTTTGTCGATTTTGTTTACCTAATAACTACACTGCAATTCCGGAACGTTAAGTAATTGGCTGTCAACTAGAGGAAATACACCTTCACTCTATGATCTGAATACTTAATTCCTTCTAATGTCTTAGAACTGGACTCATGAAATGAGACCAAAGTCATTGCATAGTTGCATCTTCATGTTTCAGAAAGATTATTTTGATGAAGTTAGAATGTGTATAATACAACTTCAGATTCAATGTTGGTTGTAGATTTTTACTTCCAGGTTTCTTATTGATAGTAGAAGTAAAAGATTCTTTCTTTCAAAGAAGTGAAGTGATTGAGATTATACCCATCTTGTCGACCTATTTTGGCAAGATGATGTAATTTTGAAACTGATCTGAGACGTTATTTTCCAGGTTCACCGTTGGCCTGGTTTTACTTCGTCGAGGATTTGATGCAAACCGTCATTGCTCTTGGTGCCATTATTTGTCTTGCGTTCCTACTTCGAAATGGAGCTGTAATTCAGAACCTACATACTGTTTGGTAA

mRNA sequence

TGTTCCGCGCGGTTCGGAGAAACGGGACAAGGACTTGGAGAGAATTGAATCAAACCGAGGCTTATTTTGATCTCAATCGTCATCTTCGACCGTCGATCAAGAACCAACTACTCTTGTCTTCGTTCTCTGTAGCGGAGCCCTAGTTGCCATCGTCGATTAAGTTTATATTCATCCAACCTGAGTTCATGACAAGATGTCAGGCACTGGGGAAAGATATCTCCAAGACGACGCCGATGAAGCAGCTGATGAAAATGGCAAGCAGTCCAGCGCACAAGAGTGGACGGTAGCTATCGCTGCAAAAGGGCCTCTGTGAAAAGTCAGGAAGGATGGCTGTTTGACGACCTGACTTTTTCGAAGGTCGGTGGGACATGGGGGCCTCACCTCATGCATCCGTTTAAAGTAGAGGAAGTATATACAGAATTAGCAAAAGTTGAGAGTAGGTCAACCAATTTATTTGAAGATTCTGATCGTTGAATTTCTTAAACTGGAGTTGCTAGCTCAACTGCAACCGCCTGATCTGAGGTGTCCCCTTGTTGCAACTAATCATACAGCCAAGAATTTACAGATGCATAATAGCAATTGAAGTATTGAAGAGTACAGTATGTAAACATTATACGGCATGTTATATTTAATATTATTTTGACAATTTTGTTTATGTTTGAAATGAATAGCTATTCTTATAGTTGTAAACATGTGAAAAGTTCATTTCTTGTTTGATTGATTGGAAAAGGAGACGAAAAAACGAAGTTAATTGGGTTCTGAATTTTCTTCCTCTTTGGGATTTGATAATTGGCCCCTGCAAAATGGACTATTGCTGTATGATAACTTGAGCAACCAAAGAAAGAAGATCCTTCGCCTGAGAGACTTGTAACACGAGCCATCTCTCGTTATGAACAATTTGGCGTCTCTCATGTATCTCAAGCCGCCATGGTAGGTGGCCTTTGATTCTTGCACCTTCAAATTATGACTTGAGCTTCAATGGCGGAACATCACCCTTCGGATATTCAAATCATGGTCAATTCCCGGCAAGGTACTGCAATACATCCTGTAGAGGTGGATGCCCAGTCGTTGCAAGCGTCGGGTTCAATTGCTTACAGGGAAGTCAAGCAATTCAAGAAATGGATCTCTTGGTTAATTCCGTCATTTGTTATAGCCAATATTGTTATGTTCATTATCACCATGTATGTTAATAATTGCCCTAAGAATTCAGTCACTTGTATTGCTCGATTCTTGGGTAGATTCTCTTTCCAGCCCCTAAAAGAAAATCCTCTGCTTGGACCTTCATCTTTAACGCTACGGAGTATGGGTGCATTAGAAGTTAATAAAGTGGTTCATGGAGACCAACTGTGGCGTCTCGTCACGTTACGGATCGGCTTGCTATATGTTATATCGGGATTTGGTGGGAGTCTGTTGTCTGCTCTGTTCATCCAGTCAAACATCTCTGTCGGGGCTTCTGGTGCCTTGTTTGGGTTGCTCGGAGGAATGCTTTCTGAACTCATCACTAACTGGACGATATATTCTAATAAGGTACCAGCTCTTCTTACTCTGTTGGTCATTATAGCGATCAACTTGGCAGTAGGAATTCTTCCTCATGTTGATAATTTTGCTCATATTGGAGGATTTATTTCTGGATTTCTTCTGGGGTTTGTATTTCTCATTCGCCCGCAGTTTGGATGGAACTGGACTCATGAAATGAGACCAAAGTCATTGCATAGTTGCATCTTCATGTTTCAGAAAGATTATTTTGATGAAGTTAGAATGTGTATAATACAACTTCAGATTCAATGTTCACCGTTGGCCTGGTTTTACTTCGTCGAGGATTTGATGCAAACCGTCATTGCTCTTGGTGCCATTATTTGTCTTGCGTTCCTACTTCGAAATGGAGCTGTAATTCAGAACCTACATACTGTTTGGTAA

Coding sequence (CDS)

ATGGCGGAACATCACCCTTCGGATATTCAAATCATGGTCAATTCCCGGCAAGGTACTGCAATACATCCTGTAGAGGTGGATGCCCAGTCGTTGCAAGCGTCGGGTTCAATTGCTTACAGGGAAGTCAAGCAATTCAAGAAATGGATCTCTTGGTTAATTCCGTCATTTGTTATAGCCAATATTGTTATGTTCATTATCACCATGTATGTTAATAATTGCCCTAAGAATTCAGTCACTTGTATTGCTCGATTCTTGGGTAGATTCTCTTTCCAGCCCCTAAAAGAAAATCCTCTGCTTGGACCTTCATCTTTAACGCTACGGAGTATGGGTGCATTAGAAGTTAATAAAGTGGTTCATGGAGACCAACTGTGGCGTCTCGTCACGTTACGGATCGGCTTGCTATATGTTATATCGGGATTTGGTGGGAGTCTGTTGTCTGCTCTGTTCATCCAGTCAAACATCTCTGTCGGGGCTTCTGGTGCCTTGTTTGGGTTGCTCGGAGGAATGCTTTCTGAACTCATCACTAACTGGACGATATATTCTAATAAGGTACCAGCTCTTCTTACTCTGTTGGTCATTATAGCGATCAACTTGGCAGTAGGAATTCTTCCTCATGTTGATAATTTTGCTCATATTGGAGGATTTATTTCTGGATTTCTTCTGGGGTTTGTATTTCTCATTCGCCCGCAGTTTGGATGGAACTGGACTCATGAAATGAGACCAAAGTCATTGCATAGTTGCATCTTCATGTTTCAGAAAGATTATTTTGATGAAGTTAGAATGTGTATAATACAACTTCAGATTCAATGTTCACCGTTGGCCTGGTTTTACTTCGTCGAGGATTTGATGCAAACCGTCATTGCTCTTGGTGCCATTATTTGTCTTGCGTTCCTACTTCGAAATGGAGCTGTAATTCAGAACCTACATACTGTTTGGTAA

Protein sequence

MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHGDQLWRLVTLRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGWNWTHEMRPKSLHSCIFMFQKDYFDEVRMCIIQLQIQCSPLAWFYFVEDLMQTVIALGAIICLAFLLRNGAVIQNLHTVW
Homology
BLAST of Cp4.1LG03g02580 vs. ExPASy Swiss-Prot
Match: Q0WQX7 (RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 3.8e-75
Identity = 152/270 (56.30%), Postives = 186/270 (68.89%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQAS------GSIAYREVKQFKKWISWLIP 60
           MA      ++I V +    A + V V+     A+         ++ E + FK W  WL+P
Sbjct: 1   MARDRREGLEIKVVNPPAAATNNVAVETSPATATRRRRQQQRASFAEFRPFKLWFPWLVP 60

Query: 61  SFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEV 120
           + V+ANI +F I+M++NNCPKNS  C+ARFLGRF+FQP+KENPLLGPSSLTL  MGAL+V
Sbjct: 61  AIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLTLEKMGALDV 120

Query: 121 NKVVHGDQLWRLVT-------------------------------LRIGLLYVISGFGGS 180
           + VVH  ++WRL T                               +RIGLLY+ISGFGGS
Sbjct: 121 SMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIGLLYMISGFGGS 180

Query: 181 LLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGIL 234
           LLS+LF ++ ISVGASGALFGLLG MLSEL+TNWTIY+NK  ALLTL+ IIAINLAVGIL
Sbjct: 181 LLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTLIFIIAINLAVGIL 240

BLAST of Cp4.1LG03g02580 vs. ExPASy Swiss-Prot
Match: F4JBM4 (RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.4e-72
Identity = 138/225 (61.33%), Postives = 168/225 (74.67%), Query Frame = 0

Query: 40  REVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLL 99
           R VK+F+ W  WLIP FV+AN+ +F+ITMYVNNCPK S  C A FLGRFSFQ  +ENPLL
Sbjct: 56  RGVKEFRSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGDCFADFLGRFSFQNTRENPLL 115

Query: 100 GPSSLTLRSMGALEVNKVVHGDQLWRLVT------------------------------- 159
           GPSSLTL++MG L+V KVV GD+ WRL++                               
Sbjct: 116 GPSSLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGF 175

Query: 160 LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALL 219
           +RIGLLY+ISGFGGS+LSALF++SNISVGASGA+FGLLGGMLSE+  NWTIYSNKV  ++
Sbjct: 176 IRIGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIV 235

Query: 220 TLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW 234
           TL++I+A+NL +G+LP VDNFAHIGGF +GFLLGFV LIRP +GW
Sbjct: 236 TLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGW 280

BLAST of Cp4.1LG03g02580 vs. ExPASy Swiss-Prot
Match: Q9CAN1 (RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 1.5e-68
Identity = 140/251 (55.78%), Postives = 172/251 (68.53%), Query Frame = 0

Query: 33  ASGSIAYREVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKN----SVTCIARFLGRF 92
           A+ +  Y E      W SWLIP+ V+AN+ +FI  M+VN+CPK     +  C+ARFLGRF
Sbjct: 16  ANNNYFYEESSGETHWTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRF 75

Query: 93  SFQPLKENPLLGPSSLTLRSMGALEVNKVVHGDQLWRLVT-------------------- 152
           SFQPLKENPL GPSS TL  MGALE  KVVH  Q WRL++                    
Sbjct: 76  SFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIF 135

Query: 153 -----------LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNW 212
                      +R+GL+Y+ISG GGS+LS+LF+Q +ISVGASGALFGLLG MLSEL+TNW
Sbjct: 136 IGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISVGASGALFGLLGAMLSELLTNW 195

Query: 213 TIYSNKVPALLTLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW--NW 247
           TIY+NK  AL+TLL IIAINLA+G+LP VDNFAHIGGF++GF LGFV L+RPQ+GW  + 
Sbjct: 196 TIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR 255

BLAST of Cp4.1LG03g02580 vs. ExPASy Swiss-Prot
Match: Q9LYP1 (RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.0e-64
Identity = 127/237 (53.59%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 34  SGSIAYREVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKNSVT------CIARFLGR 93
           S S  + +     +W SWL+P FV+AN+ +F++ M+VNNCP +  +      C+A+FLGR
Sbjct: 39  SSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGR 98

Query: 94  FSFQPLKENPLLGPSSLTLRSMGALEVNKVVHGDQLWRLVT------------------- 153
            SF+PL+ NPL GPSS TL  +GALE +KVV   + WRL+T                   
Sbjct: 99  LSFEPLRTNPLFGPSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLV 158

Query: 154 ------------LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITN 213
                       +RIG++Y++SG GGS+LS+LFI+++ISVGASGALFGLLG MLSEL TN
Sbjct: 159 FIGIRLEQQFGFVRIGVIYLLSGIGGSVLSSLFIRNSISVGASGALFGLLGSMLSELFTN 218

Query: 214 WTIYSNKVPALLTLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW 234
           WTIYSNK+ ALLTLL +I INLA+GILPHVDNFAH+GGF++GFLLGF+ L RPQF W
Sbjct: 219 WTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKW 275

BLAST of Cp4.1LG03g02580 vs. ExPASy Swiss-Prot
Match: Q8VZ48 (RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-61
Identity = 121/222 (54.50%), Postives = 156/222 (70.27%), Query Frame = 0

Query: 47  KWISWLIPSFVIANIVMFIITMYVNNCPKNSV----TCIARFLGRFSFQPLKENPLLGPS 106
           +W +WL P+ V+AN+ +FI+ MY N+CPK +      C+A+ L RFSFQPL+ENP LGPS
Sbjct: 17  QWTAWLTPTIVVANVSIFIVVMYTNDCPKTTTGANGDCVAKLLRRFSFQPLRENPFLGPS 76

Query: 107 SLTLRSMGALEVNKVVHGDQLWRLVT-------------------------------LRI 166
           S TL  +GAL+  KVV G++ WRL+T                               +RI
Sbjct: 77  SSTLEKLGALDWKKVVQGNEKWRLITAMWLHAGIIHLVMNMFDVIIFGIRLEQQFGFIRI 136

Query: 167 GLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLL 226
           GL+Y+ISGFGGS+LSALF+Q +ISVGASGAL GL+G MLSEL+TNWTIY +K+ ALL+ L
Sbjct: 137 GLIYLISGFGGSILSALFLQKSISVGASGALLGLMGAMLSELLTNWTIYKSKLCALLSFL 196

Query: 227 VIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW 234
            IIAINLA+G+LP VDNFAHIGG ++GF LGF+ L++PQ GW
Sbjct: 197 FIIAINLAIGLLPWVDNFAHIGGLLTGFCLGFILLMQPQSGW 238

BLAST of Cp4.1LG03g02580 vs. NCBI nr
Match: KAA0044094.1 (RHOMBOID-like protein 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 440 bits (1132), Expect = 1.12e-151
Identity = 248/343 (72.30%), Postives = 260/343 (75.80%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQI  NSRQGTAIHPVE D QSLQASGS+AY EVKQF+KWI WLIPSFVIAN
Sbjct: 74  MAEHHPSDIQI--NSRQGTAIHPVEADGQSLQASGSVAYWEVKQFRKWIPWLIPSFVIAN 133

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           IVMFIITM VNNCPKNSV+CIARFLGRFSFQ LKENPLLGPSSLTLR MGALEVNKVVHG
Sbjct: 134 IVMFIITMSVNNCPKNSVSCIARFLGRFSFQSLKENPLLGPSSLTLRRMGALEVNKVVHG 193

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           +QLWRLVT                               LRIGLLYVISGFGGSLLS+LF
Sbjct: 194 NQLWRLVTCIWLHAGVFHLLANMLSLLVIGIRLEQEFGFLRIGLLYVISGFGGSLLSSLF 253

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 240
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKV AL+TLLVII INLAVGILPHVDNF
Sbjct: 254 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVAALVTLLVIIVINLAVGILPHVDNF 313

Query: 241 AHIGGFISGFLLGFVFLIRPQFGWNWTHEMRPKSLHSCIFMFQKDYFDEVRMCIIQLQIQ 300
           AHIGGFISGFLLGF+              + P+SL                     +  +
Sbjct: 314 AHIGGFISGFLLGFIV---------GCGSLVPRSL---------------------IDSK 373

Query: 301 CSPLAWFYFVEDLMQTVIALGAIICLAFLLRNGAVIQNLHTVW 312
            SPL WFYFVEDLMQTVI LGAIICLAFLL+NGAVIQNLHTVW
Sbjct: 374 SSPLVWFYFVEDLMQTVIVLGAIICLAFLLQNGAVIQNLHTVW 384

BLAST of Cp4.1LG03g02580 vs. NCBI nr
Match: XP_022934849.1 (RHOMBOID-like protein 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 436 bits (1121), Expect = 3.38e-151
Identity = 231/264 (87.50%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 6   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 65

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           I+MFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 66  IIMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 125

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 126 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 185

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 186 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 245

BLAST of Cp4.1LG03g02580 vs. NCBI nr
Match: XP_023528619.1 (RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023528620.1 RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023528621.1 RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023528622.1 RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023528623.1 RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436 bits (1122), Expect = 3.58e-150
Identity = 232/264 (87.88%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 121 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 180

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 240

BLAST of Cp4.1LG03g02580 vs. NCBI nr
Match: KAG7017291.1 (RHOMBOID-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 436 bits (1121), Expect = 5.08e-150
Identity = 231/264 (87.50%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           I+MFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 61  IIMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 121 DQLWRLVTCNWLHAGVFHLLANMLSLLVTGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 180

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 240

BLAST of Cp4.1LG03g02580 vs. NCBI nr
Match: XP_022934845.1 (RHOMBOID-like protein 1 isoform X1 [Cucurbita moschata] >XP_022934846.1 RHOMBOID-like protein 1 isoform X1 [Cucurbita moschata] >XP_022934847.1 RHOMBOID-like protein 1 isoform X1 [Cucurbita moschata] >XP_022934848.1 RHOMBOID-like protein 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 436 bits (1121), Expect = 6.04e-150
Identity = 231/264 (87.50%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 6   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 65

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           I+MFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 66  IIMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 125

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 126 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 185

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 186 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 245

BLAST of Cp4.1LG03g02580 vs. ExPASy TrEMBL
Match: A0A5A7TLL8 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G004440 PE=3 SV=1)

HSP 1 Score: 440 bits (1132), Expect = 5.42e-152
Identity = 248/343 (72.30%), Postives = 260/343 (75.80%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQI  NSRQGTAIHPVE D QSLQASGS+AY EVKQF+KWI WLIPSFVIAN
Sbjct: 74  MAEHHPSDIQI--NSRQGTAIHPVEADGQSLQASGSVAYWEVKQFRKWIPWLIPSFVIAN 133

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           IVMFIITM VNNCPKNSV+CIARFLGRFSFQ LKENPLLGPSSLTLR MGALEVNKVVHG
Sbjct: 134 IVMFIITMSVNNCPKNSVSCIARFLGRFSFQSLKENPLLGPSSLTLRRMGALEVNKVVHG 193

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           +QLWRLVT                               LRIGLLYVISGFGGSLLS+LF
Sbjct: 194 NQLWRLVTCIWLHAGVFHLLANMLSLLVIGIRLEQEFGFLRIGLLYVISGFGGSLLSSLF 253

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 240
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKV AL+TLLVII INLAVGILPHVDNF
Sbjct: 254 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVAALVTLLVIIVINLAVGILPHVDNF 313

Query: 241 AHIGGFISGFLLGFVFLIRPQFGWNWTHEMRPKSLHSCIFMFQKDYFDEVRMCIIQLQIQ 300
           AHIGGFISGFLLGF+              + P+SL                     +  +
Sbjct: 314 AHIGGFISGFLLGFIV---------GCGSLVPRSL---------------------IDSK 373

Query: 301 CSPLAWFYFVEDLMQTVIALGAIICLAFLLRNGAVIQNLHTVW 312
            SPL WFYFVEDLMQTVI LGAIICLAFLL+NGAVIQNLHTVW
Sbjct: 374 SSPLVWFYFVEDLMQTVIVLGAIICLAFLLQNGAVIQNLHTVW 384

BLAST of Cp4.1LG03g02580 vs. ExPASy TrEMBL
Match: A0A6J1F3R1 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441887 PE=3 SV=1)

HSP 1 Score: 436 bits (1121), Expect = 1.64e-151
Identity = 231/264 (87.50%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 6   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 65

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           I+MFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 66  IIMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 125

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 126 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 185

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 186 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 245

BLAST of Cp4.1LG03g02580 vs. ExPASy TrEMBL
Match: A0A6J1F8W4 (RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441887 PE=3 SV=1)

HSP 1 Score: 436 bits (1121), Expect = 2.92e-150
Identity = 231/264 (87.50%), Postives = 233/264 (88.26%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 6   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 65

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           I+MFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 66  IIMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 125

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 126 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 185

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 186 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 245

BLAST of Cp4.1LG03g02580 vs. ExPASy TrEMBL
Match: A0A6J1J374 (RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111481435 PE=3 SV=1)

HSP 1 Score: 434 bits (1116), Expect = 1.68e-149
Identity = 231/264 (87.50%), Postives = 232/264 (87.88%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQI VNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN
Sbjct: 6   MAEHHPSDIQIRVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 65

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG
Sbjct: 66  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 125

Query: 121 DQLWRLVT-------------------------------LRIGLLYVISGFGGSLLSALF 180
           DQLWRLVT                               +RIGLLYVISGFGGSLLSALF
Sbjct: 126 DQLWRLVTCNWLHAGVFHLLANMLSLLVIGIRLEQEFGFIRIGLLYVISGFGGSLLSALF 185

Query: 181 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 233
           IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF
Sbjct: 186 IQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGILPHVDNF 245

BLAST of Cp4.1LG03g02580 vs. ExPASy TrEMBL
Match: A0A5D3DN31 (RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001640 PE=3 SV=1)

HSP 1 Score: 432 bits (1110), Expect = 2.30e-148
Identity = 247/362 (68.23%), Postives = 260/362 (71.82%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQASGSIAYREVKQFKKWISWLIPSFVIAN 60
           MAEHHPSDIQI  NSRQGTAIHPVE D QSLQASGS+AY EVKQF+KWI WLIPSFVIAN
Sbjct: 74  MAEHHPSDIQI--NSRQGTAIHPVEADGQSLQASGSVAYWEVKQFRKWIPWLIPSFVIAN 133

Query: 61  IVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEVNKVVHG 120
           IVMFIITM VNNCPKNSV+CIARFLGRFSFQ LKENPLLGPSSLTLR MGALEVNKVVHG
Sbjct: 134 IVMFIITMSVNNCPKNSVSCIARFLGRFSFQSLKENPLLGPSSLTLRRMGALEVNKVVHG 193

Query: 121 DQLWRLVT--------------------------------------------------LR 180
           +QLWRLVT                                                  +R
Sbjct: 194 NQLWRLVTCIWLHAGVFHLLANMLSLLVIGIRLEQEFGFLDWINRHSTSYKFACGCFTVR 253

Query: 181 IGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTL 240
           IGLLYVISGFGGSLLS+LFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKV AL+TL
Sbjct: 254 IGLLYVISGFGGSLLSSLFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVAALVTL 313

Query: 241 LVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGWNWTHEMRPKSLHSCIFM 300
           LVII INLAVGILPHVDNFAHIGGFISGFLLGF+              + P+SL      
Sbjct: 314 LVIIVINLAVGILPHVDNFAHIGGFISGFLLGFIV---------GCGSLVPRSL------ 373

Query: 301 FQKDYFDEVRMCIIQLQIQCSPLAWFYFVEDLMQTVIALGAIICLAFLLRNGAVIQNLHT 312
                          +  + SPL WFYFVEDLMQTVI LGAIICLAFLL+NGAVIQNLHT
Sbjct: 374 ---------------IDSKSSPLVWFYFVEDLMQTVIVLGAIICLAFLLQNGAVIQNLHT 403

BLAST of Cp4.1LG03g02580 vs. TAIR 10
Match: AT2G29050.1 (RHOMBOID-like 1 )

HSP 1 Score: 283.1 bits (723), Expect = 2.7e-76
Identity = 152/270 (56.30%), Postives = 186/270 (68.89%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQAS------GSIAYREVKQFKKWISWLIP 60
           MA      ++I V +    A + V V+     A+         ++ E + FK W  WL+P
Sbjct: 1   MARDRREGLEIKVVNPPAAATNNVAVETSPATATRRRRQQQRASFAEFRPFKLWFPWLVP 60

Query: 61  SFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEV 120
           + V+ANI +F I+M++NNCPKNS  C+ARFLGRF+FQP+KENPLLGPSSLTL  MGAL+V
Sbjct: 61  AIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLTLEKMGALDV 120

Query: 121 NKVVHGDQLWRLVT-------------------------------LRIGLLYVISGFGGS 180
           + VVH  ++WRL T                               +RIGLLY+ISGFGGS
Sbjct: 121 SMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIGLLYMISGFGGS 180

Query: 181 LLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALLTLLVIIAINLAVGIL 234
           LLS+LF ++ ISVGASGALFGLLG MLSEL+TNWTIY+NK  ALLTL+ IIAINLAVGIL
Sbjct: 181 LLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKFAALLTLIFIIAINLAVGIL 240

BLAST of Cp4.1LG03g02580 vs. TAIR 10
Match: AT3G53780.2 (RHOMBOID-like protein 4 )

HSP 1 Score: 274.6 bits (701), Expect = 9.6e-74
Identity = 138/225 (61.33%), Postives = 168/225 (74.67%), Query Frame = 0

Query: 40  REVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLL 99
           R VK+F+ W  WLIP FV+AN+ +F+ITMYVNNCPK S  C A FLGRFSFQ  +ENPLL
Sbjct: 56  RGVKEFRSWFPWLIPCFVVANVAVFVITMYVNNCPKKSGDCFADFLGRFSFQNTRENPLL 115

Query: 100 GPSSLTLRSMGALEVNKVVHGDQLWRLVT------------------------------- 159
           GPSSLTL++MG L+V KVV GD+ WRL++                               
Sbjct: 116 GPSSLTLQTMGGLDVKKVVKGDEGWRLLSCNWLHGGVVHLLMNMLTLLFIGIRMEREFGF 175

Query: 160 LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKVPALL 219
           +RIGLLY+ISGFGGS+LSALF++SNISVGASGA+FGLLGGMLSE+  NWTIYSNKV  ++
Sbjct: 176 IRIGLLYLISGFGGSILSALFLRSNISVGASGAVFGLLGGMLSEIFINWTIYSNKVVTIV 235

Query: 220 TLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW 234
           TL++I+A+NL +G+LP VDNFAHIGGF +GFLLGFV LIRP +GW
Sbjct: 236 TLVLIVAVNLGLGVLPGVDNFAHIGGFATGFLLGFVLLIRPHYGW 280

BLAST of Cp4.1LG03g02580 vs. TAIR 10
Match: AT2G29050.2 (RHOMBOID-like 1 )

HSP 1 Score: 270.0 bits (689), Expect = 2.4e-72
Identity = 153/298 (51.34%), Postives = 187/298 (62.75%), Query Frame = 0

Query: 1   MAEHHPSDIQIMVNSRQGTAIHPVEVDAQSLQAS------GSIAYREVKQFKKWISWLIP 60
           MA      ++I V +    A + V V+     A+         ++ E + FK W  WL+P
Sbjct: 1   MARDRREGLEIKVVNPPAAATNNVAVETSPATATRRRRQQQRASFAEFRPFKLWFPWLVP 60

Query: 61  SFVIANIVMFIITMYVNNCPKNSVTCIARFLGRFSFQPLKENPLLGPSSLTLRSMGALEV 120
           + V+ANI +F I+M++NNCPKNS  C+ARFLGRF+FQP+KENPLLGPSSLTL  MGAL+V
Sbjct: 61  AIVVANIALFAISMFINNCPKNSAYCLARFLGRFAFQPMKENPLLGPSSLTLEKMGALDV 120

Query: 121 NKVVHGDQLWRLVT-------------------------------LRIGLLYVISGFGGS 180
           + VVH  ++WRL T                               +RIGLLY+ISGFGGS
Sbjct: 121 SMVVHKHEVWRLFTCIWLHAGVFHVLANMLSLIFIGIRLEQEFGFVRIGLLYMISGFGGS 180

Query: 181 LLSALFIQSNISVGASGALFGLLGGMLSELITNWTIYSNKV------------------- 234
           LLS+LF ++ ISVGASGALFGLLG MLSEL+TNWTIY+NKV                   
Sbjct: 181 LLSSLFNRAGISVGASGALFGLLGAMLSELLTNWTIYANKVAKSSLVKQAALSMNDVSIM 240

BLAST of Cp4.1LG03g02580 vs. TAIR 10
Match: AT1G63120.1 (RHOMBOID-like 2 )

HSP 1 Score: 261.2 bits (666), Expect = 1.1e-69
Identity = 140/251 (55.78%), Postives = 172/251 (68.53%), Query Frame = 0

Query: 33  ASGSIAYREVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKN----SVTCIARFLGRF 92
           A+ +  Y E      W SWLIP+ V+AN+ +FI  M+VN+CPK     +  C+ARFLGRF
Sbjct: 16  ANNNYFYEESSGETHWTSWLIPAIVVANLAVFIAVMFVNDCPKKITGPNKECVARFLGRF 75

Query: 93  SFQPLKENPLLGPSSLTLRSMGALEVNKVVHGDQLWRLVT-------------------- 152
           SFQPLKENPL GPSS TL  MGALE  KVVH  Q WRL++                    
Sbjct: 76  SFQPLKENPLFGPSSSTLEKMGALEWRKVVHEHQGWRLLSCMWLHAGIIHLLTNMLSLIF 135

Query: 153 -----------LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITNW 212
                      +R+GL+Y+ISG GGS+LS+LF+Q +ISVGASGALFGLLG MLSEL+TNW
Sbjct: 136 IGIRLEQQFGFIRVGLIYLISGLGGSILSSLFLQESISVGASGALFGLLGAMLSELLTNW 195

Query: 213 TIYSNKVPALLTLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW--NW 247
           TIY+NK  AL+TLL IIAINLA+G+LP VDNFAHIGGF++GF LGFV L+RPQ+GW  + 
Sbjct: 196 TIYANKAAALITLLFIIAINLALGMLPRVDNFAHIGGFLTGFCLGFVLLVRPQYGWEASR 255

BLAST of Cp4.1LG03g02580 vs. TAIR 10
Match: AT5G07250.1 (RHOMBOID-like protein 3 )

HSP 1 Score: 248.4 bits (633), Expect = 7.4e-66
Identity = 127/237 (53.59%), Postives = 164/237 (69.20%), Query Frame = 0

Query: 34  SGSIAYREVKQFKKWISWLIPSFVIANIVMFIITMYVNNCPKNSVT------CIARFLGR 93
           S S  + +     +W SWL+P FV+AN+ +F++ M+VNNCP +  +      C+A+FLGR
Sbjct: 39  SSSTEFGDNALSSRWTSWLVPMFVVANVAVFVVAMFVNNCPNHFESHRLRGHCVAKFLGR 98

Query: 94  FSFQPLKENPLLGPSSLTLRSMGALEVNKVVHGDQLWRLVT------------------- 153
            SF+PL+ NPL GPSS TL  +GALE +KVV   + WRL+T                   
Sbjct: 99  LSFEPLRTNPLFGPSSHTLEKLGALEWSKVVEKKEGWRLLTCIWLHAGVIHLGANMLSLV 158

Query: 154 ------------LRIGLLYVISGFGGSLLSALFIQSNISVGASGALFGLLGGMLSELITN 213
                       +RIG++Y++SG GGS+LS+LFI+++ISVGASGALFGLLG MLSEL TN
Sbjct: 159 FIGIRLEQQFGFVRIGVIYLLSGIGGSVLSSLFIRNSISVGASGALFGLLGSMLSELFTN 218

Query: 214 WTIYSNKVPALLTLLVIIAINLAVGILPHVDNFAHIGGFISGFLLGFVFLIRPQFGW 234
           WTIYSNK+ ALLTLL +I INLA+GILPHVDNFAH+GGF++GFLLGF+ L RPQF W
Sbjct: 219 WTIYSNKIAALLTLLFVILINLAIGILPHVDNFAHVGGFVTGFLLGFILLARPQFKW 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WQX73.8e-7556.30RHOMBOID-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RBL1 PE=2 SV=1[more]
F4JBM41.4e-7261.33RHOMBOID-like protein 4 OS=Arabidopsis thaliana OX=3702 GN=RBL4 PE=2 SV=1[more]
Q9CAN11.5e-6855.78RHOMBOID-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RBL2 PE=1 SV=1[more]
Q9LYP11.0e-6453.59RHOMBOID-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RBL3 PE=2 SV=1[more]
Q8VZ481.4e-6154.50RHOMBOID-like protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL6 P... [more]
Match NameE-valueIdentityDescription
KAA0044094.11.12e-15172.30RHOMBOID-like protein 1 isoform X2 [Cucumis melo var. makuwa][more]
XP_022934849.13.38e-15187.50RHOMBOID-like protein 1 isoform X2 [Cucurbita moschata][more]
XP_023528619.13.58e-15087.88RHOMBOID-like protein 1 [Cucurbita pepo subsp. pepo] >XP_023528620.1 RHOMBOID-li... [more]
KAG7017291.15.08e-15087.50RHOMBOID-like protein 1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022934845.16.04e-15087.50RHOMBOID-like protein 1 isoform X1 [Cucurbita moschata] >XP_022934846.1 RHOMBOID... [more]
Match NameE-valueIdentityDescription
A0A5A7TLL85.42e-15272.30RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23... [more]
A0A6J1F3R11.64e-15187.50RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441887 PE=3 SV=1[more]
A0A6J1F8W42.92e-15087.50RHOMBOID-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441887 PE=3 SV=1[more]
A0A6J1J3741.68e-14987.50RHOMBOID-like protein OS=Cucurbita maxima OX=3661 GN=LOC111481435 PE=3 SV=1[more]
A0A5D3DN312.30e-14868.23RHOMBOID-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35... [more]
Match NameE-valueIdentityDescription
AT2G29050.12.7e-7656.30RHOMBOID-like 1 [more]
AT3G53780.29.6e-7461.33RHOMBOID-like protein 4 [more]
AT2G29050.22.4e-7251.34RHOMBOID-like 1 [more]
AT1G63120.11.1e-6955.78RHOMBOID-like 2 [more]
AT5G07250.17.4e-6653.59RHOMBOID-like protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 128..228
e-value: 6.4E-23
score: 81.4
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 27..228
e-value: 2.5E-17
score: 65.1
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 48..231
IPR002610Peptidase S54, rhomboidPANTHERPTHR22936RHOMBOID-RELATEDcoord: 14..128
coord: 129..243
NoneNo IPR availablePANTHERPTHR22936:SF79RHOMBOID-LIKE PROTEIN 4coord: 14..128
coord: 129..243

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02580.1Cp4.1LG03g02580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity