Cp4.1LG03g02560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02560
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionDelta-1-pyrroline-5-carboxylate synthase
LocationCp4.1LG03: 494966 .. 499953 (+)
RNA-Seq ExpressionCp4.1LG03g02560
SyntenyCp4.1LG03g02560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGTGTGTCCTCTCCATCTTCTCCGCATAAACACTCACTGAGTTACCATACCAACGCGCTCCCCATGGATCCTTCTCGAGTTTTTACCAAGAATGTGAGGCGCCTCGTCCTCAAGGTTTCTATCCCCCTTTCTTCATTTTTACATTTCTATCATTCTTTTATTACTTTGTGTTTTTTAAATTAAAAAAAAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTGAAAACATATAAATATTGTTTTACGTATGTATGGACATAAATCGAAACAACTGAATCCATTCATATTTTTGAAGTAGTTGCATTTTTGGTGCCTTGAATTTCTCCAGAAAGTATACTTGAAATCAAGTTTCCCCCACTTTTTCTCTGCGCCTTTTATAAATACATTTACCACTTCAAATACATCTGCTTCATTGGAGTTTTTTGGAGTTTTTTTTTATTGTTCCTACAATTTTCTGCTTCGTCTGGGTCGCCTCGTATGTCCCGAGTCGACCCGGTTTCCTCTCCTTCTTTTATTCTTCTTAACCATATATACGTTACCGATGGATTCAACTGATCCTACCAGAACTTTCATAAGAAATATTAAGCGTCTGGTGGTTAAGGTACCCATCATAAATCCTACTCTGTTTTTCTTTTCTAATTCGTCTCCACATTCCCAGAAATGTGGGTTCTCGATTTTACTGCCCAAATGAAATCAATGAACAATGTTGTTACTGAAAAGGTTTTGTTTTGTTTGTGATAATGACTCAAGGTTGGGACTGCTGTGGTTACCCGTGGAGATGGGCGACTGGCATTGGGAAGGCTTGGGGCTCTGTGTGAGCAGGTGAGATTATATTTGGTTTAATTGGGAATATTTTAGATCCATTGCTTTTGTGTTTTTGAGATTAAGAACAAATTCATGGCGGAACAGTTGAAGGAGTTGAACTCTCGAGGGTACGAGGTTATTTTGGTCACATCAGGGGCTGTCGGTCTTGGTCGCCAAAGGCTCAGATACAGAAAATTGGCTAACAGCAGGTTCTCTTCTAAATTCCCCTTTTCCTTTAATTTCTTCCTTTTCTCTGTTGCTTTGTTTCTGTACCACCATCCTTCCCACGTGGACCAATGCCAAGTGTTTCCCTTTTTGATCCGATCTCTAAGGAATAATTTAGAAAATAAAAATATAAAATCTTCCTCTCACAATTCACCCCATTGGGAAATGGGGTGAACCGGTGAGTCATCTCAGCGGAAGTCTTTTTGAGAGAGGTTCCACACCTTTATAAGTAATGTTTTTCGTTCCCCTCCAATTGACGTTTGACCTGGGATCTCATATTTGAATCTTGTGAGGCGAGGATGGATCAATGTTGCTTCTCCTGAGAAGGTATATTTTTGTTTCTCCAGCTTTGCAGATCTTCAGAATCCACAAGGTGAATTTGATGGAAAGGCATGCGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGGTAATAATAGAATTGCATGATCATCTAAAAGCTCTTTATATCAACGTCAGACACAAATGCTTCCTCTGTTCAATTGTTGTTGATAATGTGTTCTTATGCAAATTTGCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGTGATACTGGGTTCAGACAACAGCTTTCTGAAACAGTTAATTCGTTGTTAGATCTTAGAGTGATTCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACTCCATATGAGGATTCTTCTGGTATCTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTGAGTGATGTTGAAGGGCTCTACAGTGGCCCTCCAATTGACCCAACCTCAAAGCTAATCCAGACTTTTGTTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAATCCAGAGTCGGCAGAGGAGGTATGACTGCTAAAGTTAATGCTGCTGTTTGTGCTTCTTGTGCTGGCATTCCTGTTGTCATTACCAGGTATACATAACTCAACCCCATCTTCTCAATTGAGCTGTGATATATCAGTCTAATTCTGATCTGATTTCTGGTGCAGTGGTTTTGCCACTGATAACATAATCAAAGTACTCCAAGGTGAGATGAAAGGAACTCTCTTCCACAAAGATGCCCATTTGTGGACTCTGGTTAAAGAAGTTTCTGCAAGAGAGATGGCGGTCGAGGCACGGGAAAGTTCGAGACGGCTTCAGGTCAGGGGAACAAACTTCCTTGATATAACTTCTGAATTATATGAGTTTGGACTAAGTTTTGTTTTTTTTTTCTTTCCAGGCTCTCAAGTCTGAGGATAGGAGAAAGATATTGCTGGATGTAGCTAATGCCCTTCAAGATAATGAAGATATGATTATGGCTGAGAACAATGCTGATATTAAAGCTGCAGAGGAGGCTGGATATGAGAAGTCTCTGATATCTCGTTTGGCTCTAAAGCCTGGAAGAGTACTGATTTTTCATCCATGGCTTCAATATCTTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAGATTGAGCTTCTTGCAAACTCCATTCGAAAAATTGCTGACATGGAAGAACCAATTGGTCGCATTTTGAAAAGAAAAGAAGTAGCAATACTACTTAGAACTTGTTTCTTTTTCTATGTTTACTGTTATATTTGAGTTCATGATGCTCCTCTACTATGCCTAGTGATATCTCTGTCATTCCATTCAGATTGCGAGTGATCTTATTTTAGAGAACACCACATGCTCCTTGGGTGTCCTCCTCGTTGTGTTTGAGTCTCGGCCCGATGCTCTAGTTCAGGTCTAATGCTCTCGATGATTCAGTTCAATCGTTCTGCTTGTTGTGAGTACACTATATTGAAGCTCATTGCCTTACAGATAGCTGCATTGGCAATACGAAGCGGAAATGGTCTGCTTCTTAAAGGAGGCAAAGAAGCCAGACAATCAAATGCAGCTCTGCATAAGGTTCATTTTCACCTGGTTATTCTAGCTTCATTATTGGCTTATTTCAATGTACCTTCCCGTTAACGTATTCGACTTTGGTTAGGTCATCACGTCGGCCATCCCCGACACCGTAGGTGGAAAACTTATTGGACTTGTAACATCAAGAGAAGAGATCCCTGATCTGCTCAAGGTTAGGAGGGAAAGAAGTTTATACTTGAATGTTGCTTATTAGCACTGAAAGAAATGTTCTTATAACAACTTGATGGCTCAGCTTGATGATGTGATAGACCTTGTGATTCCAAGAGGAAGTAACAAGCTTGTGTCTCAGATTAAAGAATCCACAAAGATTCCTGTTCTTGGCCATTCTGGTGAGACATCAAGCGCCGTGATATGCTATATATACTCTGCAAACCAGATCTACTACTGAAGATTTCTCTTTTTTTTCCCCTGCACAGATGGTATCTGTCATGTTTATGTCGACAAATCGGCTGATATAGAGATGGCAAAGCGGATAGTGTCCGATTCGAAAGTAGATTATCCAGCAGCCTGCAACGCAATGGTTTGTTTGTTTCAAAGGGCTATCTACGGTCTGCAATATGTTTCATCTTCTTGACTATATAATGTGCATTCCTTGTGTTTTTTCAGGAAACACTTCTAGTACATAAGGACTTGGCAAGTAATGGAGGACTTATGGAACTCGTTGCCGAACTGCGGAAGGAAGGTATAGATGAACCACTTCCTCTTTAACATATCTCGTATCGATTGCTCGATGTCTGGCCATGAACCTTCTGTTGCTCAAGTCTAATTGGTTTCTAATGCAGGTGTGACTCTATATGGGGGACAACGAGCTAGTCAATTACTTAGAATCAGCGAGGCAAGCTCATTCCACCACGAGTACAATTCACTGACCTGCACTGTTGAGATTGTGGATGATGTCTTTGCTGCCATTGACCACATACATCAACATGGAAGGTGACAGAATCTCCCACATGTTAGCTCTGTTTCTTGCCTCAAGGAAATAGTGAACTGAAACTAACCCCTTGATCATATCATGGCAGCTCGCATACAGACTGTATCATCACCAAAGACCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTCGATAGGTATGGTTGCATCTACCTTTTTAGTGCTCTTAACTTGTTCGAGAGGAGGTTAGAGTTTCAAGGGTTGACTCGTGTTTCCTGTTCTCTGCAACAGTGCTGCTGTATTCCACAATGCCAGTACACGATTCTGCGATGGAGCTCGGTTTGGACTTGGTGCAGAGGTAAGCCATTCCCAAACATTCATGGCCGAAAAACCAAGATGAAATCCTTACTTGTTTCTGATTTCTGGGTATAACGGCGGAACAGGTGGGAGTAAGTACCAGTAGGATTCATGCGCGAGGCCCAGTAGGAGTTGAAGGACTGTTGACAACAAGATGGTAAGATGATTTCTGCAAATAATGTTTGAAAACAGGGATGGTTTGACAAGTTTAAAGTCCATTTTGGTGATGATGTTGTGTGTTTGTGTAAATGCAGGATTTTAAGGGGAAATGGGCAAGTAGTGAACGGCGACAAAGGAGTGACCTACATCCACAAAGATCTCACCCAGAAAAATTGATGGGAGTCGCCACAATGCTCTTGAAGATTCATTTCTTATACCAGCCCATGTTATCATCTCACATTTATTTTTGTCAGTCATTTCCCTTCCTTAAGTTTTATTGCAATCTAGAGATTGAGCTAAGATTTCAACTTAGAGTCAGTCGCAAACTCTTTTGCTTTACTTTTGTAATCATAAACATGACAAAATAATTCAAAAAAGAAAAATGGATTATTTCTTGCCTTACCTCCATTACTCTCCAAATAAGTTCCGTTCTCGCCGATACTCATCTAATTATCGATCAAAAGGCTAAAACGTTCAAACCTACATCACACAATACCTGTTAGCACTTCGAAGGGTGCA

mRNA sequence

CTGTGTGTCCTCTCCATCTTCTCCGCATAAACACTCACTGAGTTACCATACCAACGCGCTCCCCATGGATCCTTCTCGAGTTTTTACCAAGAATGTGAGGCGCCTCGTCCTCAAGGTTGGGACTGCTGTGGTTACCCGTGGAGATGGGCGACTGGCATTGGGAAGGCTTGGGGCTCTGTGTGAGCAGTTGAAGGAGTTGAACTCTCGAGGGTACGAGGTTATTTTGGTCACATCAGGGGCTGTCGGTCTTGGTCGCCAAAGGCTCAGATACAGAAAATTGGCTAACAGCAGCTTTGCAGATCTTCAGAATCCACAAGGTGAATTTGATGGAAAGGCATGCGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGTGATACTGGGTTCAGACAACAGCTTTCTGAAACAGTTAATTCGTTGTTAGATCTTAGAGTGATTCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACTCCATATGAGGATTCTTCTGGTATCTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTGAGTGATGTTGAAGGGCTCTACAGTGGCCCTCCAATTGACCCAACCTCAAAGCTAATCCAGACTTTTGTTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAATCCAGAGTCGGCAGAGGAGGTATGACTGCTAAAGTTAATGCTGCTGTTTGTGCTTCTTGTGCTGGCATTCCTGTTGTCATTACCAGTGGTTTTGCCACTGATAACATAATCAAAGTACTCCAAGGTGAGATGAAAGGAACTCTCTTCCACAAAGATGCCCATTTGTGGACTCTGGTTAAAGAAGTTTCTGCAAGAGAGATGGCGGTCGAGGCACGGGAAAGTTCGAGACGGCTTCAGGCTCTCAAGTCTGAGGATAGGAGAAAGATATTGCTGGATATTGCGAGTGATCTTATTTTAGAGAACACCACATGCTCCTTGGGTGTCCTCCTCGTTGTGTTTGAGTCTCGGCCCGATGCTCTAGTTCAGATAGCTGCATTGGCAATACGAAGCGGAAATGGTCTGCTTCTTAAAGGAGGCAAAGAAGCCAGACAATCAAATGCAGCTCTGCATAAGGTCATCACGTCGGCCATCCCCGACACCGTAGGTGGAAAACTTATTGGACTTGTAACATCAAGAGAAGAGATCCCTGATCTGCTCAAGCTTGATGATGTGATAGACCTTGTGATTCCAAGAGGAAGTAACAAGCTTGTGTCTCAGATTAAAGAATCCACAAAGATTCCTGTTCTTGGCCATTCTGATGGTATCTGTCATGTTTATGTCGACAAATCGGCTGATATAGAGATGGCAAAGCGGATAGTGTCCGATTCGAAAGTAGATTATCCAGCAGCCTGCAACGCAATGGAAACACTTCTAGTACATAAGGACTTGGCAAGTAATGGAGGACTTATGGAACTCGTTGCCGAACTGCGGAAGGAAGGTGTGACTCTATATGGGGGACAACGAGCTAGTCAATTACTTAGAATCAGCGAGGCAAGCTCATTCCACCACGAGTACAATTCACTGACCTGCACTGTTGAGATTGTGGATGATGTCTTTGCTGCCATTGACCACATACATCAACATGGAAGCTCGCATACAGACTGTATCATCACCAAAGACCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTCGATAGTGCTGCTGTATTCCACAATGCCAGTACACGATTCTGCGATGGAGCTCGGTTTGGACTTGGTGCAGAGGTGGGAGTAAGTACCAGTAGGATTCATGCGCGAGGCCCAGTAGGAGTTGAAGGACTGTTGACAACAAGATGGATTTTAAGGGGAAATGGGCAAGTAGTGAACGGCGACAAAGGAGTGACCTACATCCACAAAGATCTCACCCAGAAAAATTGATGGGAGTCGCCACAATGCTCTTGAAGATTCATTTCTTATACCAGCCCATGTTATCATCTCACATTTATTTTTGTCAGTCATTTCCCTTCCTTAAGTTTTATTGCAATCTAGAGATTGAGCTAAGATTTCAACTTAGAGTCAGTCGCAAACTCTTTTGCTTTACTTTTGTAATCATAAACATGACAAAATAATTCAAAAAAGAAAAATGGATTATTTCTTGCCTTACCTCCATTACTCTCCAAATAAGTTCCGTTCTCGCCGATACTCATCTAATTATCGATCAAAAGGCTAAAACGTTCAAACCTACATCACACAATACCTGTTAGCACTTCGAAGGGTGCA

Coding sequence (CDS)

ATGGATCCTTCTCGAGTTTTTACCAAGAATGTGAGGCGCCTCGTCCTCAAGGTTGGGACTGCTGTGGTTACCCGTGGAGATGGGCGACTGGCATTGGGAAGGCTTGGGGCTCTGTGTGAGCAGTTGAAGGAGTTGAACTCTCGAGGGTACGAGGTTATTTTGGTCACATCAGGGGCTGTCGGTCTTGGTCGCCAAAGGCTCAGATACAGAAAATTGGCTAACAGCAGCTTTGCAGATCTTCAGAATCCACAAGGTGAATTTGATGGAAAGGCATGCGCAGCTGTTGGGCAGAGTAGCCTCATGGCTCTTTATGATACCATGTTTAGCCAGCTTGATGTGACCTCTTCCCAACTTCTAGTGACAGATTCAGTTTTTTGTGATACTGGGTTCAGACAACAGCTTTCTGAAACAGTTAATTCGTTGTTAGATCTTAGAGTGATTCCCATTTTTAATGAAAATGATGCAGTTAGTACTAGGAAAACTCCATATGAGGATTCTTCTGGTATCTTCTGGGATAATGACAGTTTGGCAGGTCTATTAGCTTTAGAACTCAAAGCTGATCTTCTGGTGTTACTGAGTGATGTTGAAGGGCTCTACAGTGGCCCTCCAATTGACCCAACCTCAAAGCTAATCCAGACTTTTGTTAAAGAGAAACATTTAGGGGAAATAACATTTGGTGACAAATCCAGAGTCGGCAGAGGAGGTATGACTGCTAAAGTTAATGCTGCTGTTTGTGCTTCTTGTGCTGGCATTCCTGTTGTCATTACCAGTGGTTTTGCCACTGATAACATAATCAAAGTACTCCAAGGTGAGATGAAAGGAACTCTCTTCCACAAAGATGCCCATTTGTGGACTCTGGTTAAAGAAGTTTCTGCAAGAGAGATGGCGGTCGAGGCACGGGAAAGTTCGAGACGGCTTCAGGCTCTCAAGTCTGAGGATAGGAGAAAGATATTGCTGGATATTGCGAGTGATCTTATTTTAGAGAACACCACATGCTCCTTGGGTGTCCTCCTCGTTGTGTTTGAGTCTCGGCCCGATGCTCTAGTTCAGATAGCTGCATTGGCAATACGAAGCGGAAATGGTCTGCTTCTTAAAGGAGGCAAAGAAGCCAGACAATCAAATGCAGCTCTGCATAAGGTCATCACGTCGGCCATCCCCGACACCGTAGGTGGAAAACTTATTGGACTTGTAACATCAAGAGAAGAGATCCCTGATCTGCTCAAGCTTGATGATGTGATAGACCTTGTGATTCCAAGAGGAAGTAACAAGCTTGTGTCTCAGATTAAAGAATCCACAAAGATTCCTGTTCTTGGCCATTCTGATGGTATCTGTCATGTTTATGTCGACAAATCGGCTGATATAGAGATGGCAAAGCGGATAGTGTCCGATTCGAAAGTAGATTATCCAGCAGCCTGCAACGCAATGGAAACACTTCTAGTACATAAGGACTTGGCAAGTAATGGAGGACTTATGGAACTCGTTGCCGAACTGCGGAAGGAAGGTGTGACTCTATATGGGGGACAACGAGCTAGTCAATTACTTAGAATCAGCGAGGCAAGCTCATTCCACCACGAGTACAATTCACTGACCTGCACTGTTGAGATTGTGGATGATGTCTTTGCTGCCATTGACCACATACATCAACATGGAAGCTCGCATACAGACTGTATCATCACCAAAGACCAGGAAGTTGCTGAAATTTTCTTGAATCAAGTCGATAGTGCTGCTGTATTCCACAATGCCAGTACACGATTCTGCGATGGAGCTCGGTTTGGACTTGGTGCAGAGGTGGGAGTAAGTACCAGTAGGATTCATGCGCGAGGCCCAGTAGGAGTTGAAGGACTGTTGACAACAAGATGGATTTTAAGGGGAAATGGGCAAGTAGTGAACGGCGACAAAGGAGTGACCTACATCCACAAAGATCTCACCCAGAAAAATTGA

Protein sequence

MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDIASDLILENTTCSLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQVVNGDKGVTYIHKDLTQKN
Homology
BLAST of Cp4.1LG03g02560 vs. ExPASy Swiss-Prot
Match: O04015 (Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 2.0e-280
Identity = 506/711 (71.17%), Postives = 571/711 (80.31%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D +R F K V+R+++KVGTAVVTR DGRLALGRLGALCEQ+ ELNS+G+EVILVTSGAV
Sbjct: 4   VDSTRAFVKGVKRVIIKVGTAVVTRADGRLALGRLGALCEQIHELNSQGFEVILVTSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYRKL NSSFADLQ PQ E DGKACAAVGQ+ L+ALYDT+FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLVNSSFADLQKPQIELDGKACAAVGQNGLLALYDTLFSQLDVTSAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL+ETV SLL+L+VIPIFNENDAVSTRK PYED+SGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPEFRKQLTETVESLLNLKVIPIFNENDAVSTRKAPYEDASGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPP DP SKLI T++KE   G ITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVEGLYSGPPSDPQSKLIHTYIKEMFEGLITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPVVITSG+AT+NIIKVLQGE  GTLFH+DA  W  V +V AR+MAV AR
Sbjct: 244 KAAVYAAHAGIPVVITSGYATNNIIKVLQGERIGTLFHRDAQKWAPVGDVGARDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDIA-------------------------------------- 360
           ESSRRLQA+  +DR KILLD+A                                      
Sbjct: 304 ESSRRLQAMSPQDRSKILLDVADALEANEKLIRIENEADLAAAQQAGYEKSLISRLALKS 363

Query: 361 ------------------------------SDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                           L+LE T+  LGVLL++FESRPDALVQIA
Sbjct: 364 GKISSLAKSIRVLANMEEPIGHVLKRTEITDGLVLEKTSSPLGVLLIIFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGL+LKGGKEA++SNA LHKVITSAIP+ VG +LIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLVLKGGKEAKRSNAILHKVITSAIPENVGPRLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGH+DGICHVYVDKSA+++MAK++V D+K DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKESTKIPVLGHADGICHVYVDKSANMDMAKKVVLDAKTDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  NG L EL+ EL+ +GV ++GG RAS LL I EA S HHEY+SL CT
Sbjct: 544 NAMETLLVHKDLVQNGCLDELIVELQIKGVVIHGGPRASSLLHIPEARSLHHEYSSLACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 644
           +EIVDDV+AAIDHIH+HGS+HTD IIT+D EVAEIFL QVDS++V HNASTRF DGARFG
Sbjct: 604 IEIVDDVYAAIDHIHRHGSAHTDSIITEDHEVAEIFLRQVDSSSVLHNASTRFSDGARFG 663

BLAST of Cp4.1LG03g02560 vs. ExPASy Swiss-Prot
Match: O65361 (Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=3544 GN=P5CS PE=2 SV=1)

HSP 1 Score: 963.8 bits (2490), Expect = 1.0e-279
Identity = 505/712 (70.93%), Postives = 566/712 (79.49%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MD +R F K+V+R+V+KVGTAVVTR DGRLALGRLG+LCEQLKELNS GYEVILVTSGAV
Sbjct: 1   MDATRAFVKDVKRVVVKVGTAVVTRSDGRLALGRLGSLCEQLKELNSDGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
             GRQRLR+RKL NSSFADLQ PQ E DGKACAAVGQ+ LMALYDT+FSQLD+T++QLLV
Sbjct: 61  SAGRQRLRFRKLVNSSFADLQKPQVELDGKACAAVGQNGLMALYDTLFSQLDLTAAQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR QL+ETV  LLDL+V+P+ NENDAVSTRK PYEDSSGIFWDNDSLA LL
Sbjct: 121 TDNDFRDPSFRTQLTETVYQLLDLKVVPVLNENDAVSTRKAPYEDSSGIFWDNDSLAALL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDV+GLY+GPP DP SKLI T+VKEKH GEITFGDKSR+GRGGMTAKV
Sbjct: 181 ALELKADLLILLSDVDGLYNGPPSDPRSKLISTYVKEKHQGEITFGDKSRLGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPV+I SG ATDNIIKV+ G+  GTLFHKDAHLW  VKE   R+MAV AR
Sbjct: 241 KAAVYAAYAGIPVIIASGKATDNIIKVIDGQCVGTLFHKDAHLWVQVKETGVRDMAVAAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQA+ SE+R+KILLD                                        
Sbjct: 301 ESSRRLQAVSSEERKKILLDIADALEANEEKILAENEADVAAAQYAGYDRSLVARLAMNP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IA  LILE T+C LGVLL+VFESRPDALVQIA
Sbjct: 361 DKISSLAKSIRVLADMEEPIGRILKRTEIADGLILEKTSCPLGVLLIVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEA++SNA LHKVITSAIPD VG KLIGLVTSR+EIPDLLKLDDV
Sbjct: 421 SLAIRSGNGLLLKGGKEAKRSNAILHKVITSAIPDKVGEKLIGLVTSRDEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKEST+IPVLGH+DGICHVYVDKSA+++MAKRIV D+K DYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTRIPVLGHADGICHVYVDKSANMDMAKRIVLDAKTDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQR--ASQLLRISEASSFHHEYNSLT 600
           NAMETLLVHKDLA NGGL +L+ +LR EGVT++GG R  A Q   I    +F+ EY+S  
Sbjct: 541 NAMETLLVHKDLAENGGLNDLIVDLRTEGVTMFGGPRIDALQEFNIQATQTFNREYSSPA 600

Query: 601 CTVEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGAR 643
           CTVEIVDDV+AAI+HI+ HGS+HTDCII +D +VAE FL  VDSAAV HNASTRFCDG R
Sbjct: 601 CTVEIVDDVYAAIEHINHHGSAHTDCIIAEDHKVAETFLQLVDSAAVLHNASTRFCDGFR 660

BLAST of Cp4.1LG03g02560 vs. ExPASy Swiss-Prot
Match: Q96480 (Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2 PE=2 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 1.7e-279
Identity = 503/713 (70.55%), Postives = 571/713 (80.08%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D +R F KNV+RL++KVGTAVVTR DGRLALGRLGALCEQL+ELNS+GYEVILVTSGAV
Sbjct: 4   VDSTRAFVKNVKRLIVKVGTAVVTRADGRLALGRLGALCEQLQELNSQGYEVILVTSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           G+GRQRLRYRKL NSSF DLQ PQ E DGKACAAVGQ+ LMALYD++FSQLDVTS+QLLV
Sbjct: 64  GVGRQRLRYRKLLNSSFLDLQKPQTELDGKACAAVGQNGLMALYDSLFSQLDVTSAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TD+ F D  FR+QL++TVNSLL L+VIPIFNENDA+STR+ PYEDSSGIFWDNDSLA LL
Sbjct: 124 TDNDFRDPDFRRQLNDTVNSLLSLKVIPIFNENDAISTRRAPYEDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDV+GLYSGPP DP SKLI T++KE H   ITFGDKSRVGRGGMTAKV
Sbjct: 184 ALELKADLLVLLSDVDGLYSGPPRDPDSKLIYTYIKEIHERVITFGDKSRVGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AA+ A+ AGIPVVITSGFATDNIIKVL GE  GTLFH DA+ W  + E  AREMAV AR
Sbjct: 244 KAAMYAAYAGIPVVITSGFATDNIIKVLHGERIGTLFHCDANKWASIGETDAREMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDIASDL----------------------------------- 360
             SRRLQAL S++R KIL DIA  L                                   
Sbjct: 304 ACSRRLQALSSQERSKILQDIADALEANEKAILAENEADVVAAQQAGYEKSLISRLALNP 363

Query: 361 ---------------------------------ILENTTCSLGVLLVVFESRPDALVQIA 420
                                            ILE ++  LGV+L++FESRPDALVQIA
Sbjct: 364 GKISSLANSVRVLSNMDEPLGHTLKRTEIADGFILEKSSSPLGVVLIIFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LA+RSGNGL+LKGGKEA++SNA LHKVITSAIP +VG +LIGLVTSREEIP+LLKLDDV
Sbjct: 424 SLAVRSGNGLMLKGGKEAKRSNAILHKVITSAIPVSVGERLIGLVTSREEIPELLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYVDKSAD++MAKRI  D+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKASTKIPVLGHADGICHVYVDKSADMDMAKRITVDAKIDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLA NGGL +L+ EL+ +GV+LYGG +AS LL I EA +F HEY+SL CT
Sbjct: 544 NAMETLLVHKDLAQNGGLNDLIVELQTKGVSLYGGPKASSLLMIPEARTFRHEYSSLACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VE+V+DV+AAIDHIHQHGS+HTD IIT+DQEVAE+FL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYAAIDHIHQHGSAHTDSIITEDQEVAEVFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. ExPASy Swiss-Prot
Match: P54887 (Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5CSA PE=1 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 2.3e-276
Identity = 497/710 (70.00%), Postives = 563/710 (79.30%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F ++V+R+V+KVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPP DP SKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW  + + +AR+MAV AR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDI--------------------------------------- 360
           ESSR+LQAL SEDR+KILLDI                                       
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 -----------------------------ASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                        A  L+LE T+  LGVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ D +MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTLYGG RAS++L I EA SF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VE+V+DV+ AIDHIH+HGS+HTDCI+T+D EVAE+FL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. ExPASy Swiss-Prot
Match: P54888 (Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana OX=3702 GN=P5CSB PE=2 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 3.0e-276
Identity = 496/710 (69.86%), Postives = 564/710 (79.44%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F K+V+R+V+KVGTAVVT   GRLALGRLGA+CEQL ELNS G+EVILV+SGAV
Sbjct: 4   IDRSRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT +Q+LV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDS F D  FR+QLSETV ++L +RVIP+FNENDA+STR+ PY+DS+GIFWDNDSLA LL
Sbjct: 124 TDSSFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           +LELKADLL+LLSDVEGLY+GPP D TSKLI TF+KEKH  EITFG+KS++GRGGMTAKV
Sbjct: 184 SLELKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+  G+PV+ITSG+A +NI KVL+G   GTLFH+DAHLW  V + ++R+MAV AR
Sbjct: 244 KAAVNAAYGGVPVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDIAS------------------------------------- 360
           ESSR+LQAL SEDR++IL DIA+                                     
Sbjct: 304 ESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKP 363

Query: 361 -------------------------------DLILENTTCSLGVLLVVFESRPDALVQIA 420
                                          DLILE T+  +GVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYVDKS  ++MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  NG L +L+  L+ +GVTLYGG RAS  L I E  SFHHEY+S  CT
Sbjct: 544 NAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VEIV+DV+ AIDHIHQHGS+HTDCI+T+D EVAEIFL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. NCBI nr
Match: XP_023529106.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1224 bits (3167), Expect = 0.0
Identity = 646/714 (90.48%), Postives = 646/714 (90.48%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

BLAST of Cp4.1LG03g02560 vs. NCBI nr
Match: XP_022935070.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 644/714 (90.20%), Postives = 646/714 (90.48%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGG+RASQLLRISEASSFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGERASQLLRISEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIHQ+GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

BLAST of Cp4.1LG03g02560 vs. NCBI nr
Match: XP_023529105.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1213 bits (3139), Expect = 0.0
Identity = 638/713 (89.48%), Postives = 643/713 (90.18%), Query Frame = 0

Query: 2   DPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG 61
           DP+R F +N++RLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG
Sbjct: 5   DPTRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG 64

Query: 62  LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT 121
           LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT
Sbjct: 65  LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT 124

Query: 122 DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA 181
           DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA
Sbjct: 125 DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA 184

Query: 182 LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN 241
           LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN
Sbjct: 185 LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN 244

Query: 242 AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE 301
           AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE
Sbjct: 245 AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE 304

Query: 302 SSRRLQALKSEDRRKILLD----------------------------------------- 361
           SSRRLQALKSEDRRKILLD                                         
Sbjct: 305 SSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKPG 364

Query: 362 ---------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIAA 421
                                      IASDLILENTTCSLGVLLVVFESRPDALVQIAA
Sbjct: 365 RIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIAA 424

Query: 422 LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI 481
           LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI
Sbjct: 425 LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI 484

Query: 482 DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN 541
           DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN
Sbjct: 485 DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN 544

Query: 542 AMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTV 601
           AMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTV
Sbjct: 545 AMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTV 604

Query: 602 EIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL 646
           EIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL
Sbjct: 605 EIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL 664

BLAST of Cp4.1LG03g02560 vs. NCBI nr
Match: XP_022982662.1 (delta-1-pyrroline-5-carboxylate synthase-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 639/714 (89.50%), Postives = 645/714 (90.34%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSRVFT+NVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTRNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFA DNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 241 NAAVCASCAGIPVVITSGFAADNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNENMIIAENCADIKAAEEAGYEKSLISRLALKP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRREIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRK+GVTLYGGQRASQLLRISEASSFHHEYNS+TCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKDGVTLYGGQRASQLLRISEASSFHHEYNSVTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIH++GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHKYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

BLAST of Cp4.1LG03g02560 vs. NCBI nr
Match: KAG6580519.1 (Delta-1-pyrroline-5-carboxylate synthase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1210 bits (3130), Expect = 0.0
Identity = 636/713 (89.20%), Postives = 643/713 (90.18%), Query Frame = 0

Query: 2   DPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG 61
           DP+R F +N++RLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG
Sbjct: 5   DPTRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVG 64

Query: 62  LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT 121
           LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT
Sbjct: 65  LGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVT 124

Query: 122 DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA 181
           DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA
Sbjct: 125 DSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLA 184

Query: 182 LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN 241
           LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN
Sbjct: 185 LELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVN 244

Query: 242 AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE 301
           AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE
Sbjct: 245 AAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARE 304

Query: 302 SSRRLQALKSEDRRKILLD----------------------------------------- 361
           SSRRLQALKSEDRRKILLD                                         
Sbjct: 305 SSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKPG 364

Query: 362 ---------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIAA 421
                                      IASDLILENTTCSLGVLLVVFESRPDALVQIAA
Sbjct: 365 RIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIAA 424

Query: 422 LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI 481
           LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI
Sbjct: 425 LAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVI 484

Query: 482 DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN 541
           DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN
Sbjct: 485 DLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACN 544

Query: 542 AMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTV 601
           AMETLLVHKDLASNGGLMELVAELRKEGVTLYGG+RASQLLRISEASSFHHEYNSLTCTV
Sbjct: 545 AMETLLVHKDLASNGGLMELVAELRKEGVTLYGGERASQLLRISEASSFHHEYNSLTCTV 604

Query: 602 EIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL 646
           EIVDDVFAAIDHIHQ+GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL
Sbjct: 605 EIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGL 664

BLAST of Cp4.1LG03g02560 vs. ExPASy TrEMBL
Match: A0A6J1F9I8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111442049 PE=3 SV=1)

HSP 1 Score: 1221 bits (3158), Expect = 0.0
Identity = 644/714 (90.20%), Postives = 646/714 (90.48%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGG+RASQLLRISEASSFHHEYNSLTCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGERASQLLRISEASSFHHEYNSLTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIHQ+GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

BLAST of Cp4.1LG03g02560 vs. ExPASy TrEMBL
Match: A0A6J1IX71 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111481470 PE=3 SV=1)

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 639/714 (89.50%), Postives = 645/714 (90.34%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           MDPSRVFT+NVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 1   MDPSRVFTRNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFA DNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 241 NAAVCASCAGIPVVITSGFAADNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 301 ESSRRLQALKSEDRRKILLDVANALQDNENMIIAENCADIKAAEEAGYEKSLISRLALKP 360

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 361 GRIELLANSIRKIADMEEPIGRILKRREIASDLILENTTCSLGVLLVVFESRPDALVQIA 420

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRK+GVTLYGGQRASQLLRISEASSFHHEYNS+TCT
Sbjct: 541 NAMETLLVHKDLASNGGLMELVAELRKDGVTLYGGQRASQLLRISEASSFHHEYNSVTCT 600

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIH++GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 601 VEIVDDVFAAIDHIHKYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 660

BLAST of Cp4.1LG03g02560 vs. ExPASy TrEMBL
Match: A0A6J1F4C7 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111442049 PE=3 SV=1)

HSP 1 Score: 1206 bits (3119), Expect = 0.0
Identity = 635/711 (89.31%), Postives = 641/711 (90.15%), Query Frame = 0

Query: 4   SRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           SR F +N++RLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG
Sbjct: 7   SRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 66

Query: 64  RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS
Sbjct: 67  RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 126

Query: 124 VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
           VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE
Sbjct: 127 VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA 243
           LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA
Sbjct: 187 LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA 246

Query: 244 VCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS 303
           VCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS
Sbjct: 247 VCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS 306

Query: 304 RRLQALKSEDRRKILLD------------------------------------------- 363
           RRLQALKSEDRRKILLD                                           
Sbjct: 307 RRLQALKSEDRRKILLDVANALQDNEDMIMAENNADIKAAEEAGYEKSLISRLALKPGRI 366

Query: 364 -------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIAALA 423
                                    IASDLILENTTCSLGVLLVVFESRPDALVQIAALA
Sbjct: 367 ELLANSIRKIADMEEPIGRILKRKEIASDLILENTTCSLGVLLVVFESRPDALVQIAALA 426

Query: 424 IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL 483
           IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL
Sbjct: 427 IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL 486

Query: 484 VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM 543
           VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM
Sbjct: 487 VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM 546

Query: 544 ETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTVEI 603
           ETLLVHKDLASNGGLMELVAELRKEGVTLYGG+RASQLLRISEASSFHHEYNSLTCTVEI
Sbjct: 547 ETLLVHKDLASNGGLMELVAELRKEGVTLYGGERASQLLRISEASSFHHEYNSLTCTVEI 606

Query: 604 VDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA 646
           VDDVFAAIDHIHQ+GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA
Sbjct: 607 VDDVFAAIDHIHQYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA 666

BLAST of Cp4.1LG03g02560 vs. ExPASy TrEMBL
Match: A0A6J1J3D4 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111481470 PE=3 SV=1)

HSP 1 Score: 1204 bits (3114), Expect = 0.0
Identity = 632/714 (88.52%), Postives = 643/714 (90.06%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +DP+R F +N++RLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV
Sbjct: 66  IDPTRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 125

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV
Sbjct: 126 GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 185

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL
Sbjct: 186 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 245

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV
Sbjct: 246 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 305

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
           NAAVCASCAGIPVVITSGFA DNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR
Sbjct: 306 NAAVCASCAGIPVVITSGFAADNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 365

Query: 301 ESSRRLQALKSEDRRKILLD---------------------------------------- 360
           ESSRRLQALKSEDRRKILLD                                        
Sbjct: 366 ESSRRLQALKSEDRRKILLDVANALQDNENMIIAENCADIKAAEEAGYEKSLISRLALKP 425

Query: 361 ----------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                       IASDLILENTTCSLGVLLVVFESRPDALVQIA
Sbjct: 426 GRIELLANSIRKIADMEEPIGRILKRREIASDLILENTTCSLGVLLVVFESRPDALVQIA 485

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 486 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 545

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC
Sbjct: 546 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 605

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDLASNGGLMELVAELRK+GVTLYGGQRASQLLRISEASSFHHEYNS+TCT
Sbjct: 606 NAMETLLVHKDLASNGGLMELVAELRKDGVTLYGGQRASQLLRISEASSFHHEYNSVTCT 665

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 646
           VEIVDDVFAAIDHIH++GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG
Sbjct: 666 VEIVDDVFAAIDHIHKYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 725

BLAST of Cp4.1LG03g02560 vs. ExPASy TrEMBL
Match: A0A6J1IZY8 (Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111481470 PE=3 SV=1)

HSP 1 Score: 1199 bits (3102), Expect = 0.0
Identity = 631/711 (88.75%), Postives = 640/711 (90.01%), Query Frame = 0

Query: 4   SRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 63
           SR F +N++RLV+KVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG
Sbjct: 7   SRTFIRNIKRLVVKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAVGLG 66

Query: 64  RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 123
           RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS
Sbjct: 67  RQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLVTDS 126

Query: 124 VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 183
           VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE
Sbjct: 127 VFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLLALE 186

Query: 184 LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA 243
           LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA
Sbjct: 187 LKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKVNAA 246

Query: 244 VCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS 303
           VCASCAGIPVVITSGFA DNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS
Sbjct: 247 VCASCAGIPVVITSGFAADNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEARESS 306

Query: 304 RRLQALKSEDRRKILLD------------------------------------------- 363
           RRLQALKSEDRRKILLD                                           
Sbjct: 307 RRLQALKSEDRRKILLDVANALQDNENMIIAENCADIKAAEEAGYEKSLISRLALKPGRI 366

Query: 364 -------------------------IASDLILENTTCSLGVLLVVFESRPDALVQIAALA 423
                                    IASDLILENTTCSLGVLLVVFESRPDALVQIAALA
Sbjct: 367 ELLANSIRKIADMEEPIGRILKRREIASDLILENTTCSLGVLLVVFESRPDALVQIAALA 426

Query: 424 IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL 483
           IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL
Sbjct: 427 IRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDVIDL 486

Query: 484 VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM 543
           VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM
Sbjct: 487 VIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAACNAM 546

Query: 544 ETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCTVEI 603
           ETLLVHKDLASNGGLMELVAELRK+GVTLYGGQRASQLLRISEASSFHHEYNS+TCTVEI
Sbjct: 547 ETLLVHKDLASNGGLMELVAELRKDGVTLYGGQRASQLLRISEASSFHHEYNSVTCTVEI 606

Query: 604 VDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA 646
           VDDVFAAIDHIH++GSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA
Sbjct: 607 VDDVFAAIDHIHKYGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFGLGA 666

BLAST of Cp4.1LG03g02560 vs. TAIR 10
Match: AT2G39800.1 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 952.6 bits (2461), Expect = 1.6e-277
Identity = 497/710 (70.00%), Postives = 563/710 (79.30%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F ++V+R+V+KVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPP DP SKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW  + + +AR+MAV AR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDI--------------------------------------- 360
           ESSR+LQAL SEDR+KILLDI                                       
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 -----------------------------ASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                        A  L+LE T+  LGVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ D +MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTLYGG RAS++L I EA SF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VE+V+DV+ AIDHIH+HGS+HTDCI+T+D EVAE+FL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. TAIR 10
Match: AT2G39800.4 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 952.6 bits (2461), Expect = 1.6e-277
Identity = 497/710 (70.00%), Postives = 563/710 (79.30%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F ++V+R+V+KVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPP DP SKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW  + + +AR+MAV AR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDI--------------------------------------- 360
           ESSR+LQAL SEDR+KILLDI                                       
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 -----------------------------ASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                        A  L+LE T+  LGVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ D +MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTLYGG RAS++L I EA SF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VE+V+DV+ AIDHIH+HGS+HTDCI+T+D EVAE+FL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. TAIR 10
Match: AT3G55610.1 (delta 1-pyrroline-5-carboxylate synthase 2 )

HSP 1 Score: 952.2 bits (2460), Expect = 2.1e-277
Identity = 496/710 (69.86%), Postives = 564/710 (79.44%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F K+V+R+V+KVGTAVVT   GRLALGRLGA+CEQL ELNS G+EVILV+SGAV
Sbjct: 4   IDRSRAFAKDVKRIVVKVGTAVVTGKGGRLALGRLGAICEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT +Q+LV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQMELDGKACAGVGQSSLMAYYETMFDQLDVTVAQMLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
           TDS F D  FR+QLSETV ++L +RVIP+FNENDA+STR+ PY+DS+GIFWDNDSLA LL
Sbjct: 124 TDSSFRDKDFRKQLSETVKAMLRMRVIPVFNENDAISTRRAPYKDSTGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           +LELKADLL+LLSDVEGLY+GPP D TSKLI TF+KEKH  EITFG+KS++GRGGMTAKV
Sbjct: 184 SLELKADLLILLSDVEGLYTGPPSDSTSKLIHTFIKEKHQDEITFGEKSKLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+  G+PV+ITSG+A +NI KVL+G   GTLFH+DAHLW  V + ++R+MAV AR
Sbjct: 244 KAAVNAAYGGVPVIITSGYAAENISKVLRGLRVGTLFHQDAHLWAPVVDTTSRDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDIAS------------------------------------- 360
           ESSR+LQAL SEDR++IL DIA+                                     
Sbjct: 304 ESSRKLQALSSEDRKQILHDIANALEVNEKTIKAENDLDVAAAQEAGYEESLVARLVMKP 363

Query: 361 -------------------------------DLILENTTCSLGVLLVVFESRPDALVQIA 420
                                          DLILE T+  +GVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRQLAEMEDPIGRVLKKTQVADDLILEKTSSPIGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLVSQIK STKIPVLGH+DGICHVYVDKS  ++MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVSQIKNSTKIPVLGHADGICHVYVDKSGKLDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  NG L +L+  L+ +GVTLYGG RAS  L I E  SFHHEY+S  CT
Sbjct: 544 NAMETLLVHKDLEQNGFLDDLIYVLQTKGVTLYGGPRASAKLNIPETKSFHHEYSSKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VEIV+DV+ AIDHIHQHGS+HTDCI+T+D EVAEIFL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEIVEDVYGAIDHIHQHGSAHTDCIVTEDSEVAEIFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. TAIR 10
Match: AT2G39800.3 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 941.0 bits (2431), Expect = 4.9e-274
Identity = 495/710 (69.72%), Postives = 560/710 (78.87%), Query Frame = 0

Query: 1   MDPSRVFTKNVRRLVLKVGTAVVTRGDGRLALGRLGALCEQLKELNSRGYEVILVTSGAV 60
           +D SR F ++V+R+V+KVGTAVVT   GRLALGRLGALCEQL ELNS G+EVILV+SGAV
Sbjct: 4   LDRSRAFARDVKRIVVKVGTAVVTGKGGRLALGRLGALCEQLAELNSDGFEVILVSSGAV 63

Query: 61  GLGRQRLRYRKLANSSFADLQNPQGEFDGKACAAVGQSSLMALYDTMFSQLDVTSSQLLV 120
           GLGRQRLRYR+L NSSFADLQ PQ E DGKACA VGQSSLMA Y+TMF QLDVT++QLLV
Sbjct: 64  GLGRQRLRYRQLVNSSFADLQKPQTELDGKACAGVGQSSLMAYYETMFDQLDVTAAQLLV 123

Query: 121 TDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRKTPYEDSSGIFWDNDSLAGLL 180
            DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+ PY+DSSGIFWDNDSLA LL
Sbjct: 124 NDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRRAPYQDSSGIFWDNDSLAALL 183

Query: 181 ALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHLGEITFGDKSRVGRGGMTAKV 240
           ALELKADLL+LLSDVEGLY+GPP DP SKLI TFVKEKH  EITFGDKSR+GRGGMTAKV
Sbjct: 184 ALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQDEITFGDKSRLGRGGMTAKV 243

Query: 241 NAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKDAHLWTLVKEVSAREMAVEAR 300
            AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+DA LW  + + +AR+MAV AR
Sbjct: 244 KAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQDARLWAPITDSNARDMAVAAR 303

Query: 301 ESSRRLQALKSEDRRKILLDI--------------------------------------- 360
           ESSR+LQAL SEDR+KILLDI                                       
Sbjct: 304 ESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDVASAQEAGLEESMVARLVMTP 363

Query: 361 -----------------------------ASDLILENTTCSLGVLLVVFESRPDALVQIA 420
                                        A  L+LE T+  LGVLL+VFESRPDALVQIA
Sbjct: 364 GKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSSPLGVLLIVFESRPDALVQIA 423

Query: 421 ALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGKLIGLVTSREEIPDLLKLDDV 480
           +LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGKLIGLVTSREEIPDLLKLDDV
Sbjct: 424 SLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGKLIGLVTSREEIPDLLKLDDV 483

Query: 481 IDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSADIEMAKRIVSDSKVDYPAAC 540
           IDLVIPRGSNKLV+QIK +TKIPVL   DGICHVYVDK+ D +MAKRIVSD+K+DYPAAC
Sbjct: 484 IDLVIPRGSNKLVTQIKNTTKIPVL---DGICHVYVDKACDTDMAKRIVSDAKLDYPAAC 543

Query: 541 NAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQLLRISEASSFHHEYNSLTCT 600
           NAMETLLVHKDL  N  L EL+  L+  GVTLYGG RAS++L I EA SF+HEY +  CT
Sbjct: 544 NAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASKILNIPEARSFNHEYCAKACT 603

Query: 601 VEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQVDSAAVFHNASTRFCDGARFG 643
           VE+V+DV+ AIDHIH+HGS+HTDCI+T+D EVAE+FL QVDSAAVFHNASTRF DG RFG
Sbjct: 604 VEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQVDSAAVFHNASTRFSDGFRFG 663

BLAST of Cp4.1LG03g02560 vs. TAIR 10
Match: AT2G39800.2 (delta1-pyrroline-5-carboxylate synthase 1 )

HSP 1 Score: 803.5 bits (2074), Expect = 1.2e-232
Identity = 419/610 (68.69%), Postives = 476/610 (78.03%), Query Frame = 0

Query: 101 MALYDTMFSQLDVTSSQLLVTDSVFCDTGFRQQLSETVNSLLDLRVIPIFNENDAVSTRK 160
           MA Y+TMF QLDVT++QLLV DS F D  FR+QL+ETV S+LDLRVIPIFNENDA+STR+
Sbjct: 1   MAYYETMFDQLDVTAAQLLVNDSSFRDKDFRKQLNETVKSMLDLRVIPIFNENDAISTRR 60

Query: 161 TPYEDSSGIFWDNDSLAGLLALELKADLLVLLSDVEGLYSGPPIDPTSKLIQTFVKEKHL 220
            PY+DSSGIFWDNDSLA LLALELKADLL+LLSDVEGLY+GPP DP SKLI TFVKEKH 
Sbjct: 61  APYQDSSGIFWDNDSLAALLALELKADLLILLSDVEGLYTGPPSDPNSKLIHTFVKEKHQ 120

Query: 221 GEITFGDKSRVGRGGMTAKVNAAVCASCAGIPVVITSGFATDNIIKVLQGEMKGTLFHKD 280
            EITFGDKSR+GRGGMTAKV AAV A+ AGIPV+ITSG++ +NI KVL+G   GTLFH+D
Sbjct: 121 DEITFGDKSRLGRGGMTAKVKAAVNAAYAGIPVIITSGYSAENIDKVLRGLRVGTLFHQD 180

Query: 281 AHLWTLVKEVSAREMAVEARESSRRLQALKSEDRRKILLDI------------------- 340
           A LW  + + +AR+MAV ARESSR+LQAL SEDR+KILLDI                   
Sbjct: 181 ARLWAPITDSNARDMAVAARESSRKLQALSSEDRKKILLDIADALEANVTTIKAENELDV 240

Query: 341 -------------------------------------------------ASDLILENTTC 400
                                                            A  L+LE T+ 
Sbjct: 241 ASAQEAGLEESMVARLVMTPGKISSLAASVRKLADMEDPIGRVLKKTEVADGLVLEKTSS 300

Query: 401 SLGVLLVVFESRPDALVQIAALAIRSGNGLLLKGGKEARQSNAALHKVITSAIPDTVGGK 460
            LGVLL+VFESRPDALVQIA+LAIRSGNGLLLKGGKEAR+SNA LHKVIT AIP+TVGGK
Sbjct: 301 PLGVLLIVFESRPDALVQIASLAIRSGNGLLLKGGKEARRSNAILHKVITDAIPETVGGK 360

Query: 461 LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVSQIKESTKIPVLGHSDGICHVYVDKSA 520
           LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLV+QIK +TKIPVLGH+DGICHVYVDK+ 
Sbjct: 361 LIGLVTSREEIPDLLKLDDVIDLVIPRGSNKLVTQIKNTTKIPVLGHADGICHVYVDKAC 420

Query: 521 DIEMAKRIVSDSKVDYPAACNAMETLLVHKDLASNGGLMELVAELRKEGVTLYGGQRASQ 580
           D +MAKRIVSD+K+DYPAACNAMETLLVHKDL  N  L EL+  L+  GVTLYGG RAS+
Sbjct: 421 DTDMAKRIVSDAKLDYPAACNAMETLLVHKDLEQNAVLNELIFALQSNGVTLYGGPRASK 480

Query: 581 LLRISEASSFHHEYNSLTCTVEIVDDVFAAIDHIHQHGSSHTDCIITKDQEVAEIFLNQV 640
           +L I EA SF+HEY +  CTVE+V+DV+ AIDHIH+HGS+HTDCI+T+D EVAE+FL QV
Sbjct: 481 ILNIPEARSFNHEYCAKACTVEVVEDVYGAIDHIHRHGSAHTDCIVTEDHEVAELFLRQV 540

Query: 641 DSAAVFHNASTRFCDGARFGLGAEVGVSTSRIHARGPVGVEGLLTTRWILRGNGQVVNGD 643
           DSAAVFHNASTRF DG RFGLGAEVGVST RIHARGPVGVEGLLTTRWI+RG GQVV+GD
Sbjct: 541 DSAAVFHNASTRFSDGFRFGLGAEVGVSTGRIHARGPVGVEGLLTTRWIMRGKGQVVDGD 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O040152.0e-28071.17Delta-1-pyrroline-5-carboxylate synthase OS=Actinidia deliciosa OX=3627 PE=2 SV=... [more]
O653611.0e-27970.93Delta-1-pyrroline-5-carboxylate synthase OS=Mesembryanthemum crystallinum OX=354... [more]
Q964801.7e-27970.55Delta-1-pyrroline-5-carboxylate synthase OS=Solanum lycopersicum OX=4081 GN=PRO2... [more]
P548872.3e-27670.00Delta-1-pyrroline-5-carboxylate synthase A OS=Arabidopsis thaliana OX=3702 GN=P5... [more]
P548883.0e-27669.86Delta-1-pyrroline-5-carboxylate synthase B OS=Arabidopsis thaliana OX=3702 GN=P5... [more]
Match NameE-valueIdentityDescription
XP_023529106.10.090.48delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Cucurbita pepo subsp. ... [more]
XP_022935070.10.090.20delta-1-pyrroline-5-carboxylate synthase-like isoform X2 [Cucurbita moschata][more]
XP_023529105.10.089.48delta-1-pyrroline-5-carboxylate synthase-like isoform X1 [Cucurbita pepo subsp. ... [more]
XP_022982662.10.089.50delta-1-pyrroline-5-carboxylate synthase-like isoform X3 [Cucurbita maxima][more]
KAG6580519.10.089.20Delta-1-pyrroline-5-carboxylate synthase 1, partial [Cucurbita argyrosperma subs... [more]
Match NameE-valueIdentityDescription
A0A6J1F9I80.090.20Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1IX710.089.50Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1F4C70.089.31Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1J3D40.088.52Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A6J1IZY80.088.75Delta-1-pyrroline-5-carboxylate synthase OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
Match NameE-valueIdentityDescription
AT2G39800.11.6e-27770.00delta1-pyrroline-5-carboxylate synthase 1 [more]
AT2G39800.41.6e-27770.00delta1-pyrroline-5-carboxylate synthase 1 [more]
AT3G55610.12.1e-27769.86delta 1-pyrroline-5-carboxylate synthase 2 [more]
AT2G39800.34.9e-27469.72delta1-pyrroline-5-carboxylate synthase 1 [more]
AT2G39800.21.2e-23268.69delta1-pyrroline-5-carboxylate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 292..312
NoneNo IPR availablePIRSRPIRSR016496-1PIRSR016496-1coord: 14..277
e-value: 6.5E-13
score: 46.0
NoneNo IPR availablePANTHERPTHR11063GLUTAMATE SEMIALDEHYDE DEHYDROGENASEcoord: 1..324
NoneNo IPR availablePANTHERPTHR11063:SF18DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASEcoord: 322..645
NoneNo IPR availablePANTHERPTHR11063GLUTAMATE SEMIALDEHYDE DEHYDROGENASEcoord: 322..645
NoneNo IPR availablePANTHERPTHR11063:SF18DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASEcoord: 1..324
IPR001057Glutamate/acetylglutamate kinasePRINTSPR00474GLU5KINASEcoord: 231..251
score: 55.2
coord: 133..154
score: 56.4
coord: 93..121
score: 55.43
coord: 172..199
score: 64.55
coord: 49..63
score: 64.94
IPR016162Aldehyde dehydrogenase, N-terminalGENE3D3.40.605.10Aldehyde Dehydrogenase; Chain A, domain 1coord: 320..446
e-value: 2.1E-42
score: 147.3
IPR000965GPR domainTIGRFAMTIGR00407TIGR00407coord: 323..618
e-value: 7.3E-111
score: 368.7
IPR000965GPR domainHAMAPMF_00412ProAcoord: 210..629
score: 27.636274
IPR000965GPR domainCDDcd07079ALDH_F18-19_ProA-GPRcoord: 293..625
e-value: 1.10902E-162
score: 470.377
IPR016163Aldehyde dehydrogenase, C-terminalGENE3D3.40.309.10Aldehyde Dehydrogenase; Chain A, domain 2coord: 447..591
e-value: 7.5E-60
score: 203.2
IPR036393Acetylglutamate kinase-like superfamilyGENE3D3.40.1160.10coord: 3..283
e-value: 1.7E-74
score: 252.7
IPR036393Acetylglutamate kinase-like superfamilySUPERFAMILY53633Carbamate kinase-likecoord: 12..279
IPR001048Aspartate/glutamate/uridylate kinasePFAMPF00696AA_kinasecoord: 13..257
e-value: 1.6E-32
score: 113.1
IPR005766Delta l-pyrroline-5-carboxylate synthetasePIRSFPIRSF036429P5C_synthetasecoord: 319..646
e-value: 3.4E-182
score: 605.0
coord: 1..326
e-value: 6.9E-149
score: 494.9
IPR005715Glutamate 5-kinase/delta-1-pyrroline-5-carboxylate synthaseTIGRFAMTIGR01027TIGR01027coord: 12..281
e-value: 1.0E-59
score: 200.5
IPR005715Glutamate 5-kinase/delta-1-pyrroline-5-carboxylate synthaseHAMAPMF_00456ProBcoord: 12..281
score: 28.879255
IPR020593Gamma-glutamyl phosphate reductase GPR, conserved sitePROSITEPS01223PROAcoord: 536..558
IPR019797Glutamate 5-kinase, conserved sitePROSITEPS00902GLUTAMATE_5_KINASEcoord: 229..246
IPR041744Bifunctional delta 1-pyrroline-5-carboxylate synthetase, glutamate-5-kinase domainCDDcd04256AAK_P5CS_ProBAcoord: 4..278
e-value: 4.00379E-153
score: 441.486
IPR016161Aldehyde/histidinol dehydrogenaseSUPERFAMILY53720ALDH-likecoord: 315..628

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02560.1Cp4.1LG03g02560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055129 L-proline biosynthetic process
biological_process GO:0016310 phosphorylation
biological_process GO:0006561 proline biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004349 glutamate 5-kinase activity
molecular_function GO:0004350 glutamate-5-semialdehyde dehydrogenase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor