Cp4.1LG03g02420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationCp4.1LG03: 510602 .. 512101 (-)
RNA-Seq ExpressionCp4.1LG03g02420
SyntenyCp4.1LG03g02420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACATCAAACGATACAAAATAAGAGATTTGACATGCAAGTAGCATGTCGCTATGGACAGTAAGATTTGAACAACACACTCATATAGTACCCTCGTGGAAGGAAGAAGGAGGACGACATTAATTGTGAGAATCTTTATCTCTCCATATTTTAATATTATCTTTTCAATCTCTTAAAATATCCATTTCATTCTTCAATTCGCTGCAGATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGGTCGCCCTCCAATCATTATCAATCCCACTTCTCATACCGTTCATCTGTTTATGTTCTTACTTGTTTTGTTTTATTTTGTTTTCAGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTAATTCTATCTCTCATTTATTTTCTTTACAAACCCAAAATGCTAAATTCTGAAAATGGGTGGTTGGTTTTAAGGTGAACAACGCAGGGACGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCGGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGTATGGGGTTAGATAAATCTGTGGGTAATGAATGATGGGAAGATTCAAGATTGTTCTTTGACGGTTTGTGGGTTTGTATTTCGATTGCAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTGTCAACGCTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTCAGTATGGGCTGTGCATTTGTGTGGTGTAATGTAGCAATGTTTTCAGTTTATGTGTATCATCGGAAGTGGTCAAAGCCAGAAAGCAAAACGTTAAACAGTAGGCACAGTGTCTCAGTCTGAAAGAAAATAAAATGTATGAATATCGGCCATGTGATGAGATAAGGTCCGCCTTTGAGCTTTCATTTTCTATTTTAAGTTTTGGACAAAAGATGAATTAATATCATATCCTCCTCGATGGTTGAATTAAAACCCAAAGCA

mRNA sequence

GAAAACATCAAACGATACAAAATAAGAGATTTGACATGCAAGTAGCATGTCGCTATGGACAGTAAGATTTGAACAACACACTCATATAGTACCCTCGTGGAAGGAAGAAGGAGGACGACATTAATTATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGACGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCGGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTGTCAACGCTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAGACTTCAGTATGGGCTGTGCATTTGTGTGGTGTAATGTAGCAATGTTTTCAGTTTATGTGTATCATCGGAAGTGGTCAAAGCCAGAAAGCAAAACGTTAAACAGTAGGCACAGTGTCTCAGTCTGAAAGAAAATAAAATGTATGAATATCGGCCATGTGATGAGATAAGGTCCGCCTTTGAGCTTTCATTTTCTATTTTAAGTTTTGGACAAAAGATGAATTAATATCATATCCTCCTCGATGGTTGAATTAAAACCCAAAGCA

Coding sequence (CDS)

ATGAGTTCCCCGATTCAAAGATGGTCTCTCAACGGCTTCACCGCCCTCGTCACCGGCGGAACCCGAGGCATTGGATACGCCATAGTTGAAGAGCTTGCTTCACTTGGGGCTGCCGTACACACCTGTTCGCGTAACCAAACAGAGCTGGACCAAAGGGTTAAAGAATGGGAGCGAAAGGGGTTCGAAGTCAGTGGCTCTGTTTGTGATCTCTCTTCAAGATCTCAGCGAGAGCAGCTCATTACCACTGTCTCTTCCATCTTCAATGGAAAGCTCAACATTCTCGTGAACAACGCAGGGACGGTAACACTGAAATCAGCAGCAGAGCACACAACCGAAGATTACAATAATATAATGAGCACCAATTTTGAGGCCCCTTATCATCTCTCCCAAATTTCGTATCCATTTTTGAAGGCGTCTGGTTATGGAAGTATTGTTTTTGTGTCGTCCGTTGCGGGAGTCGTCGCTTTACCAAAAGTATCAATCTATGCGGCATCCAAAGGGGCGATTAACCAAATCACGAAGAACTTGGCGTGCGAATGGGCGAAGGATAATATTCGTGTCAACGCTGTGGCTCCGTGGGCCGTGAAGACTACAATTTCTAAGCCGGATGCGGCGGTTGTGGAGGAATATAGTCGGCTGCTGGAGCGGACGCCGGCGGGTCGAATTGGAGAGGCGAATGAGATATCTTCAGTGGTGGCGTTTCTGTGCCTTCCTGCGGCTTCTTACGTGACTGGGCAGGTCATTTGTGTGGATGGTGGGTACACAGCCGGTGGGGGCTAG

Protein sequence

MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTGQVICVDGGYTAGGG
Homology
BLAST of Cp4.1LG03g02420 vs. ExPASy Swiss-Prot
Match: P50165 (Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.8e-86
Identity = 163/256 (63.67%), Postives = 199/256 (77.73%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIGYAIVEELA+ GA V+TCSR+Q +LD+ +++W RKGF+VSG
Sbjct: 11  RRWSLRGMTALVTGGTRGIGYAIVEELANFGAEVYTCSRSQNDLDECLEKWRRKGFKVSG 70

Query: 66  SVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAP 125
            VCD+SS SQR+ L+ +V+S FNGKLNIL+NNAGT   K A   T EDY+ IM TNFEA 
Sbjct: 71  PVCDVSSISQRQTLMESVTSSFNGKLNILINNAGTTIPKEATNFTAEDYSIIMGTNFEAS 130

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           Y+L Q+++P LKASG  SIVF SS AGV+A+P  SIYAASKGAINQ+TK+LACEWAKD+I
Sbjct: 131 YNLCQLAHPLLKASGNASIVFNSSAAGVIAVPLSSIYAASKGAINQVTKSLACEWAKDSI 190

Query: 186 RVNAVAPWAVKTTISKPDAAVVEE---YSRLLERTPAGRIGEANEISSVVAFLCLPAASY 245
           RVNAVAPW + T I +    V  +      L+ R P  R GE +E+SS+V +LCLP ASY
Sbjct: 191 RVNAVAPWIINTPIIEAACQVPSQKKNIESLIGRAPMKRAGEPSEVSSLVTYLCLPTASY 250

Query: 246 VTGQVICVDGGYTAGG 259
           +TGQ+ICVDGGYT  G
Sbjct: 251 ITGQIICVDGGYTVNG 266

BLAST of Cp4.1LG03g02420 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 7.5e-85
Identity = 151/253 (59.68%), Postives = 199/253 (78.66%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R QRE+L+ TVSS+F GKLNILVNN GT+ LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTG 245
           R NA+ PW + T +     +V E      ERTP GR+GEANE+S +VAFLCLPAASY+TG
Sbjct: 183 RTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITG 242

Query: 246 QVICVDGGYTAGG 259
           QVICVDGG T  G
Sbjct: 243 QVICVDGGLTVNG 255

BLAST of Cp4.1LG03g02420 vs. ExPASy Swiss-Prot
Match: P50162 (Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 9.8e-85
Identity = 159/257 (61.87%), Postives = 199/257 (77.43%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIGYAIVEELA LGA V+TCSRN+ ELD+ ++ W  KG  V GS
Sbjct: 16  RWSLKGTTALVTGGSKGIGYAIVEELAGLGARVYTCSRNEKELDECLEIWREKGLNVEGS 75

Query: 67  VCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCDL SR++R++L+ TV+ +F+GKLNILVNNAG V  K A + T +DYN IM TNFEA Y
Sbjct: 76  VCDLLSRTERDKLMQTVAHVFDGKLNILVNNAGVVIHKEAKDFTEKDYNIIMGTNFEAAY 135

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI+YP LKAS  G+++F+SS+AG  ALP VS+Y+ASKGAINQ+TK+LACEWAKDNIR
Sbjct: 136 HLSQIAYPLLKASQNGNVIFLSSIAGFSALPSVSLYSASKGAINQMTKSLACEWAKDNIR 195

Query: 187 VNAVAPWAVKT----TISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASY 246
           VN+VAP  + T    T  K +    EE    + +TP GR G+  E+S+++AFLC PAASY
Sbjct: 196 VNSVAPGVILTPLVETAIKKNPHQKEEIDNFIVKTPMGRAGKPQEVSALIAFLCFPAASY 255

Query: 247 VTGQVICVDGGYTAGGG 260
           +TGQ+I  DGG+TA GG
Sbjct: 256 ITGQIIWADGGFTANGG 272

BLAST of Cp4.1LG03g02420 vs. ExPASy Swiss-Prot
Match: Q9ZW04 (Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g29170 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.2e-84
Identity = 154/249 (61.85%), Postives = 197/249 (79.12%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+RN+T+L + V+EW+ KGFEV+ S
Sbjct: 13  RWSLGGMTALVTGGSKGLGEAVVEELAMLGARVHTCARNETQLQECVREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QRE+L+  V+SIF GKLNILVNNAGT   K   E+T +DY+ +M+TN ++ +
Sbjct: 73  VCDVSSRDQREKLMENVASIFQGKLNILVNNAGTGITKPTTEYTAQDYSFLMATNLDSAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ+++P LKASG GSIV +SS AGVV +   SIY A+KGA+NQ+ KNLACEWA+DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLMSSTAGVVHINVGSIYGATKGAMNQLAKNLACEWARDNIR 192

Query: 187 VNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTGQ 246
           VN+V PW + T +   D  + +E  R   +TP GR+G ANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWFIATPLYLNDEELKKEVER---KTPMGRVGNANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYT 256
            ICVDGG+T
Sbjct: 253 TICVDGGFT 258

BLAST of Cp4.1LG03g02420 vs. ExPASy Swiss-Prot
Match: Q9ZW03 (Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g29150 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.7e-84
Identity = 155/252 (61.51%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R++T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QRE+L+ TVSS+F GKLNILVNNAGT  +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASKGA+NQ+ ++LACEWA DNIR
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASKGAMNQLGRSLACEWASDNIR 192

Query: 187 VNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTGQ 246
           VN+V PW + T ++    +  +    + ++TP GR+GEANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG +  G
Sbjct: 253 TICVDGGASVNG 263

BLAST of Cp4.1LG03g02420 vs. NCBI nr
Match: XP_023527559.1 (tropinone reductase homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 503 bits (1295), Expect = 2.36e-179
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SYVTGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. NCBI nr
Match: XP_022983913.1 (tropinone reductase homolog [Cucurbita maxima])

HSP 1 Score: 495 bits (1275), Expect = 2.65e-176
Identity = 253/259 (97.68%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS REQLITTVSSIFNGKLNILVNNAGT+TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYMTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. NCBI nr
Match: KAG6580523.1 (hypothetical protein SDJN03_20525, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 495 bits (1275), Expect = 2.65e-176
Identity = 252/259 (97.30%), Postives = 257/259 (99.23%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQR+QLITTVSSIFNGKLNILVNNAG VTLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTIS+PDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SYVTGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. NCBI nr
Match: KAG7017273.1 (hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495 bits (1275), Expect = 3.18e-176
Identity = 252/259 (97.30%), Postives = 257/259 (99.23%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 6   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 65

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQR+QLITTVSSIFNGKLNILVNNAG VTLKSAAEHTTEDYNNIMST
Sbjct: 66  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 125

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 126 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 185

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTIS+PDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 186 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 245

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SYVTGQVICVDGGYTAGGG
Sbjct: 246 SYVTGQVICVDGGYTAGGG 264

BLAST of Cp4.1LG03g02420 vs. NCBI nr
Match: XP_022934187.1 (tropinone reductase homolog [Cucurbita moschata])

HSP 1 Score: 493 bits (1268), Expect = 3.09e-175
Identity = 251/259 (96.91%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQR+QLITTVSSIFNGKLNILVNNAG VTLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTIS+PDAAVVEEYSRLLE TPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SYVTGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. ExPASy TrEMBL
Match: A0A6J1J921 (tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 1.28e-176
Identity = 253/259 (97.68%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSR QTELDQRVKEWERKG
Sbjct: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRTQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRS REQLITTVSSIFNGKLNILVNNAGT+TLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSHREQLITTVSSIFNGKLNILVNNAGTITLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SY+TGQVICVDGGYTAGGG
Sbjct: 241 SYMTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. ExPASy TrEMBL
Match: A0A6J1F1Z8 (tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 SV=1)

HSP 1 Score: 493 bits (1268), Expect = 1.49e-175
Identity = 251/259 (96.91%), Postives = 256/259 (98.84%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           M+SPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG
Sbjct: 1   MNSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           FEVSGSVCDLSSRSQR+QLITTVSSIFNGKLNILVNNAG VTLKSAAEHTTEDYNNIMST
Sbjct: 61  FEVSGSVCDLSSRSQRQQLITTVSSIFNGKLNILVNNAGAVTLKSAAEHTTEDYNNIMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPK+SIYAASKGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKISIYAASKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPWAVKTTIS+PDAAVVEEYSRLLE TPAGRIGEANEISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWAVKTTISRPDAAVVEEYSRLLELTPAGRIGEANEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGGG 259
           SYVTGQVICVDGGYTAGGG
Sbjct: 241 SYVTGQVICVDGGYTAGGG 259

BLAST of Cp4.1LG03g02420 vs. ExPASy TrEMBL
Match: A0A5D3DN35 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001440 PE=4 SV=1)

HSP 1 Score: 443 bits (1139), Expect = 6.83e-156
Identity = 220/258 (85.27%), Postives = 243/258 (94.19%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQR+QLI TVSSIFNG LNILVNNAGTVTLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPK+SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRVN VAPW V+TTISKPDAAVVEEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGG 258
           SYV+GQ+ICVDGG+TAGG
Sbjct: 241 SYVSGQIICVDGGFTAGG 258

BLAST of Cp4.1LG03g02420 vs. ExPASy TrEMBL
Match: A0A1S3B6I2 (tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1)

HSP 1 Score: 443 bits (1139), Expect = 6.83e-156
Identity = 220/258 (85.27%), Postives = 243/258 (94.19%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSP+QRWSL+GFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLDGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQR+QLI TVSSIFNG LNILVNNAGTVTLK A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTLKGATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALPK+SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPKISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIRVN VAPW V+TTISKPDAAVVEEY RL+ RTPAGRIGE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRVNTVAPWGVRTTISKPDAAVVEEYGRLIGRTPAGRIGEPEEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGG 258
           SYV+GQ+ICVDGG+TAGG
Sbjct: 241 SYVSGQIICVDGGFTAGG 258

BLAST of Cp4.1LG03g02420 vs. ExPASy TrEMBL
Match: A0A0A0LAG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1)

HSP 1 Score: 440 bits (1131), Expect = 1.13e-154
Identity = 216/258 (83.72%), Postives = 242/258 (93.80%), Query Frame = 0

Query: 1   MSSPIQRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKG 60
           MSSP+QRWSLNGFTALVTGGTRGIGYAIVEELASLGA+VHTCSRNQTE+DQR++EW++KG
Sbjct: 1   MSSPLQRWSLNGFTALVTGGTRGIGYAIVEELASLGASVHTCSRNQTEIDQRLQEWQQKG 60

Query: 61  FEVSGSVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMST 120
           F+V+ SVCDL+S SQR+QLI TVSSIFNG LNILVNNAGTVT+K+A E+TTEDYN +MST
Sbjct: 61  FKVTASVCDLTSSSQRQQLIDTVSSIFNGTLNILVNNAGTVTMKTATEYTTEDYNYMMST 120

Query: 121 NFEAPYHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEW 180
           NFEAPYHLSQIS+P LKASGYGSIVFVSS+AGV ALP++SIYAA+KGAINQITKNLACEW
Sbjct: 121 NFEAPYHLSQISHPILKASGYGSIVFVSSIAGVTALPRISIYAATKGAINQITKNLACEW 180

Query: 181 AKDNIRVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAA 240
           AKDNIR+N VAPW V+TTIS PDAA VEEY RL+ RTPAGR+GE  EISSVVAFLCLPAA
Sbjct: 181 AKDNIRINTVAPWGVRTTISTPDAAAVEEYGRLIGRTPAGRLGEPEEISSVVAFLCLPAA 240

Query: 241 SYVTGQVICVDGGYTAGG 258
           SYV+GQ+ICVDGGYTAGG
Sbjct: 241 SYVSGQIICVDGGYTAGG 258

BLAST of Cp4.1LG03g02420 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 5.3e-86
Identity = 151/253 (59.68%), Postives = 199/253 (78.66%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G  ALVTGGT+GIG A+VEEL+ LGA VHTC+R++T+L +R++EW+ KGF+V+ 
Sbjct: 3   KRWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTT 62

Query: 66  SVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAP 125
           S+CD+S R QRE+L+ TVSS+F GKLNILVNN GT+ LK   E+T E+++ +M+TN ++ 
Sbjct: 63  SICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSA 122

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +H+SQ+++P LKASG GSIV +SS+AGVV +   SIY A+KGA+NQ+ +NLACEWA DNI
Sbjct: 123 FHISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNI 182

Query: 186 RVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTG 245
           R NA+ PW + T +     +V E      ERTP GR+GEANE+S +VAFLCLPAASY+TG
Sbjct: 183 RTNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITG 242

Query: 246 QVICVDGGYTAGG 259
           QVICVDGG T  G
Sbjct: 243 QVICVDGGLTVNG 255

BLAST of Cp4.1LG03g02420 vs. TAIR 10
Match: AT2G29150.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.6e-85
Identity = 155/252 (61.51%), Postives = 202/252 (80.16%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++G+G A+VEELA LGA VHTC+R++T+L +R++EW+ KGFEV+ S
Sbjct: 13  RWSLEGMTALVTGGSKGLGEAVVEELAMLGARVHTCARDETQLQERLREWQAKGFEVTTS 72

Query: 67  VCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR QRE+L+ TVSS+F GKLNILVNNAGT  +K + E+T EDY+ +M+TN E+ +
Sbjct: 73  VCDVSSREQREKLMETVSSVFQGKLNILVNNAGTGIIKPSTEYTAEDYSFLMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQI++P LKASG GSIVF+SSVAG+V     SIY ASKGA+NQ+ ++LACEWA DNIR
Sbjct: 133 HLSQIAHPLLKASGSGSIVFMSSVAGLVH-TGASIYGASKGAMNQLGRSLACEWASDNIR 192

Query: 187 VNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTGQ 246
           VN+V PW + T ++    +  +    + ++TP GR+GEANE+SS+VAFLC PAASY+TGQ
Sbjct: 193 VNSVCPWVITTPLTSFIFSDEKLRKAVEDKTPMGRVGEANEVSSLVAFLCFPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG +  G
Sbjct: 253 TICVDGGASVNG 263

BLAST of Cp4.1LG03g02420 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 310.8 bits (795), Expect = 1.0e-84
Identity = 157/253 (62.06%), Postives = 192/253 (75.89%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSL G TALVTGGTRGIG A+VEELA  GA VHTCSRNQ EL+  + +W+  G  VSG
Sbjct: 5   KRWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSG 64

Query: 66  SVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD S R QRE+LI   SS F+GKLNIL+NN GT   K   E+++E+Y  IMSTN E+ 
Sbjct: 65  SVCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESA 124

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +HLSQI++P LKASG GSIVF+SSVAG+V L   SIY A+KGA+NQ+T+NLACEWA DNI
Sbjct: 125 FHLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNI 184

Query: 186 RVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTG 245
           R N VAPW +KT++ +      E    ++ RTP GR+GE  E+SS+VAFLCLPA+SY+TG
Sbjct: 185 RTNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITG 244

Query: 246 QVICVDGGYTAGG 259
           QVI VDGG+T  G
Sbjct: 245 QVISVDGGFTVNG 257

BLAST of Cp4.1LG03g02420 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 8.5e-84
Identity = 149/253 (58.89%), Postives = 196/253 (77.47%), Query Frame = 0

Query: 6   QRWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSG 65
           +RWSLNG +ALVTGGTRGIG AIVEELA LGA VHTC+RN+ EL+  + +W R GF V+G
Sbjct: 64  ERWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAG 123

Query: 66  SVCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAP 125
           SVCD+S RSQRE L+ TVSS+F GKL+ILVNN GT   K   E T  +++ +MSTNFE+ 
Sbjct: 124 SVCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESV 183

Query: 126 YHLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNI 185
           +HL Q++YP L+ S  GS+VF+SSV+G V+L  +S+ +++KGAINQ+T++LACEWAKDNI
Sbjct: 184 FHLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNI 243

Query: 186 RVNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTG 245
           R+NAVAPW +KT++ +   +  E    +   TP GR+GE  E+SS VAFLCLPA+SY+TG
Sbjct: 244 RINAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITG 303

Query: 246 QVICVDGGYTAGG 259
           Q++CVDGG +  G
Sbjct: 304 QILCVDGGMSING 316

BLAST of Cp4.1LG03g02420 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 8.5e-84
Identity = 148/252 (58.73%), Postives = 196/252 (77.78%), Query Frame = 0

Query: 7   RWSLNGFTALVTGGTRGIGYAIVEELASLGAAVHTCSRNQTELDQRVKEWERKGFEVSGS 66
           RWSL G TALVTGG++GIG A+VEELA+LGA +HTC+R++T+L + +++W+ KGF+V+ S
Sbjct: 13  RWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLRKWQAKGFQVTTS 72

Query: 67  VCDLSSRSQREQLITTVSSIFNGKLNILVNNAGTVTLKSAAEHTTEDYNNIMSTNFEAPY 126
           VCD+SSR +RE+L+ TVS+IF GKLNILVNN GT  +K   +HT ED++  M+TN E+ +
Sbjct: 73  VCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDFSFTMATNLESAF 132

Query: 127 HLSQISYPFLKASGYGSIVFVSSVAGVVALPKVSIYAASKGAINQITKNLACEWAKDNIR 186
           HLSQ+++P LKASG GSIV +SSV+GVV +   SIY  SKGA+NQ+ +NLACEWA DNIR
Sbjct: 133 HLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGRNLACEWASDNIR 192

Query: 187 VNAVAPWAVKTTISKPDAAVVEEYSRLLERTPAGRIGEANEISSVVAFLCLPAASYVTGQ 246
            N+V PW ++T +     +  E    +  R P GR+GE NE+SS+VAFLCLPAASY+TGQ
Sbjct: 193 TNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAFLCLPAASYITGQ 252

Query: 247 VICVDGGYTAGG 259
            ICVDGG+T  G
Sbjct: 253 TICVDGGFTVNG 264

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P501651.8e-8663.67Tropinone reductase homolog OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Q9ZW137.5e-8559.68Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
P501629.8e-8561.87Tropinone reductase 1 OS=Datura stramonium OX=4076 GN=TR1 PE=1 SV=1[more]
Q9ZW042.2e-8461.85Tropinone reductase homolog At2g29170 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Q9ZW033.7e-8461.51Tropinone reductase homolog At2g29150 OS=Arabidopsis thaliana OX=3702 GN=At2g291... [more]
Match NameE-valueIdentityDescription
XP_023527559.12.36e-179100.00tropinone reductase homolog [Cucurbita pepo subsp. pepo][more]
XP_022983913.12.65e-17697.68tropinone reductase homolog [Cucurbita maxima][more]
KAG6580523.12.65e-17697.30hypothetical protein SDJN03_20525, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7017273.13.18e-17697.30hypothetical protein SDJN02_19136, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_022934187.13.09e-17596.91tropinone reductase homolog [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1J9211.28e-17697.68tropinone reductase homolog OS=Cucurbita maxima OX=3661 GN=LOC111482392 PE=4 SV=... [more]
A0A6J1F1Z81.49e-17596.91tropinone reductase homolog OS=Cucurbita moschata OX=3662 GN=LOC111441431 PE=4 S... [more]
A0A5D3DN356.83e-15685.27Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B6I26.83e-15685.27tropinone reductase homolog OS=Cucumis melo OX=3656 GN=LOC103486364 PE=4 SV=1[more]
A0A0A0LAG71.13e-15483.72Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G733970 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT2G29290.25.3e-8659.68NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29150.12.6e-8561.51NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G06060.11.0e-8462.06NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29260.18.5e-8458.89NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.18.5e-8458.73NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 14..31
score: 50.59
coord: 136..152
score: 35.68
coord: 218..238
score: 32.66
coord: 89..100
score: 58.08
coord: 162..181
score: 41.85
coord: 183..200
score: 40.36
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 89..100
score: 51.01
coord: 162..181
score: 38.33
coord: 142..150
score: 49.39
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 21..255
e-value: 3.5E-58
score: 196.9
NoneNo IPR availableGENE3D3.40.50.720coord: 2..259
e-value: 1.7E-81
score: 275.2
NoneNo IPR availablePIRSRPIRSR000094-3PIRSR000094-3coord: 10..255
e-value: 5.8E-19
score: 66.1
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 16..200
e-value: 1.3E-20
score: 71.6
NoneNo IPR availablePANTHERPTHR42898:SF28TROPINONE REDUCTASE-LIKE PROTEINcoord: 5..258
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 5..258
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 149..177
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 14..256

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02420.1Cp4.1LG03g02420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity