Cp4.1LG03g02320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02320
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionKinesin-like protein
LocationCp4.1LG03: 606995 .. 611189 (+)
RNA-Seq ExpressionCp4.1LG03g02320
SyntenyCp4.1LG03g02320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGAAAGGAAAAGGAAAAGGAAAAGGAAAATTTAAAATCAATAAGACTCTGCCACCAACGTCTTGATTTTCAAATTCTCCTCCCTCGTCCCTCCCTTCGCTTGGAGACTCTGCCACTGCCGACGCGAACCATACTTCCAGTTCAATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTGGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGGTACTTTGTTTTTTATTCTTTCTCTGTAGAGTGTTTTTCTGCCGTGGTAGAAGAATATTTTGGTTTGGTTGATTATAGTTGTTGGATCTTCTGTAGTCGAAACGAGTGCTATCAATTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCATGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACCATGCAGGTTATCTTTAATTAGTGGGAGATTTGTCATATGTTGCATTTAGCTTGAAATTAACAGAAGAATCATTAGCTTCATTTCATATAAGCTTTAGCTTTCCTGAAATTGATATGAAATAATTCAACTACAGAAATGACTCGCACTCTGAATTGTGTCGATTTGGTTGAGGATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACCGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGTATGTTTCTCAAGCTTAATAGAAACCTAGCCTTAACCATCAAATAAGATCTTTGCACTGCTCAAGAAGTTGTTCGTGTTGCTAATTTAGTTAATACTTGTGCATAACCATCCATCACAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTTGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGATCAAGCCGTGCCTTAATGATTGCTTGCCTGGTAAATTGATGCAGGTTAAACATCTTTTGTAATCTAAGAATTGCACTTGCTCAAATTTCCTTCTGATTTGATAACAGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGAAAGACCAAAAGTGCACAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGCAAACGGCCGACCAGAGTGACTGTAAACGAGAAAAAGAAAGGTCTGGCAATCATTTGTTGCTTTATCAACTTTTAGTACCTTCTACTAATTCTTTTAACAATACTTCATTGATGATCTTCAGGACTGTAGCCGTGGCTTTCGGATACTTGGCTGAAAATAAAGATATAGCTAATTCTATCACAACCAAAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGGTGTGCTGGAACTCTCGTTTACAGACCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAACGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGGTACTCAAAAACCCTTTTTAAATTTTCATTATCTTTATGTCTCGATGCTGTTTGGATTTCAATAATTCTGGTTTTTCTTGTCATCCTAACCAATCAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGGTAGGTCCATAATCATATACAATATTTAGCATTTTGTGCACAAGTCCTTGGTACAATATTTAGCATTTCGTGCACAAGAATATGTGGATTTCTTGTTCCAGTACCCAACTTTTCAATTTGATATAGTGAAAGTTTAATCAATTATTAATTGGCTTTCTTTGTTTTGCAGAGAGGAACTAGTAGAATTGAAGGTATTTTCTAGACTTCGACCATTTTTTTTTTCTTATTTAGGAAAAACATCAACCTCTTCCCTTTAAGTTTCCTCATTCTCTTTTCCCTTTCAATTCCCAGGGCATTGGAGTGAAAATGGCAGAGTACATTGTGGATCTCAGAGAAACTAGTCCATTGAAATCGGTAAGACAATAACCACGAGATATAAGTTTGATGTATTTCTCTCGTATGTTCAATGGTGCGTGGTGGTTTCATCCAAAATTTTCTTATAGTGGACATTCATGGTTACCAAGTCCATCTTCTCATGTCATATGCACTTGGGACCTAACCTTATCCCTTTTCTCTCCTAGTCCAATCTAAGTCTTCATTTTGGTGTCTAAGAGTTGGCCAAGCATGACCATGTCAATGACACGAGAGACCAAAACTATCTGTCACATTGTGGAGGCCTTTGTCATTCACAAATACAATCCAACCCCACACACACATAGTTTTGTCGTTTCTGATAGTGGACATTCATGGTTACCAAGTCCATCTTCTCGTGTCATATGCACTTGGGACCTAACCTTATCCCTTTTCTCTCCTAGTCCAATCTAAGTCTTCATTTTGGTGTCTAAGAGTTGGCCAAGCATGACCATGTCAATGACACGAGAGACCAAAACTATCTGTCACATTGTGGAGGCCTTTGTCATTCACAAATACAATCCAACCCCACACACACATAGTTTTGTCGTTTCTGATAGTGGACATTCATGGTTACCAAGTCCATCTTCTCGTGTCATATGCACTTGGGACCTAACCTTATCCCTTTTCTCTCCTAGTCCAATCTAAGTCTTCATTTTGGTGTCTAAGAGTTGGCCAAGCATGACCATGTCAATGACACGAGAGACCAAAACTATCCGTCACATTGTGGAGGCCTTTGCCATTCACAAATACAATCCAACCCCACACACACATAGTTTTGTCGTTTCTGATAGTCATTCGACATGTAGAAAACGTCGCATGTAAACTTACAAAAACACGTCGATAAAGCAACACTATCCAATTTCTTTCCTGAATATTTCCTGAATATAATGAACTTCTTCCCCTTGCTGTGTTTATATAGCTTGCTGACTTGAAGAAACTGGGACTCTCATCCAAGCAGGTAATGCTTCAACAAACGCAATAACTTACCAAATTTACCATTTTGTTTCACTCCTGGAAAAATCAGGCTCTCAAAATATGGCTTCTTTTTCTTCTGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGAATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCAGAAACTTCATTATCCAACCAATAATGAAGGTAGAAGAAGACATAGTAATGTATTGGGAAGTGTGCCATAGCAACATCCTCCAAGTTGCTGTATGTTAAAATATTGTAACAGTAGAGAAGAGAACTGTCCGACTAAAGATTTTGCCTGGTCGGCGTGATGGGCTTCCTTCATGTTCTCTGTAAGCCCTATCTGTATTATTCATTAACCAAATTATCTATTTGTATCACATTGAACTTGTGGCTATTGTAATCTAAATATGATGATCCAGAGTGATGCTGATTTGTCGCAATTTTTATGGTTTATATGAAACCAGAGAAGA

mRNA sequence

CGAAAGGAAAAGGAAAAGGAAAAGGAAAATTTAAAATCAATAAGACTCTGCCACCAACGTCTTGATTTTCAAATTCTCCTCCCTCGTCCCTCCCTTCGCTTGGAGACTCTGCCACTGCCGACGCGAACCATACTTCCAGTTCAATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTGGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGTCGAAACGAGTGCTATCAATTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCATGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACCATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACCGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTTGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGATCAAGCCGTGCCTTAATGATTGCTTGCCTGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGAAAGACCAAAAGTGCACAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGCAAACGGCCGACCAGAGTGACTGTAAACGAGAAAAAGAAAGGACTGTAGCCGTGGCTTTCGGATACTTGGCTGAAAATAAAGATATAGCTAATTCTATCACAACCAAAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGGTGTGCTGGAACTCTCGTTTACAGACCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAACGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGAGAGGAACTAGTAGAATTGAAGGGCATTGGAGTGAAAATGGCAGAGTACATTGTGGATCTCAGAGAAACTAGTCCATTGAAATCGCTTGCTGACTTGAAGAAACTGGGACTCTCATCCAAGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGAATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCAGAAACTTCATTATCCAACCAATAATGAAGGTAGAAGAAGACATAGTAATGTATTGGGAAGTGTGCCATAGCAACATCCTCCAAGTTGCTGTATGTTAAAATATTGTAACAGTAGAGAAGAGAACTGTCCGACTAAAGATTTTGCCTGGTCGGCGTGATGGGCTTCCTTCATGTTCTCTGTAAGCCCTATCTGTATTATTCATTAACCAAATTATCTATTTGTATCACATTGAACTTGTGGCTATTGTAATCTAAATATGATGATCCAGAGTGATGCTGATTTGTCGCAATTTTTATGGTTTATATGAAACCAGAGAAGA

Coding sequence (CDS)

ATGGCTGCTGAAAATTTTGAACTTCTTGCACCTGCTACATCCTGTGCAATCTCCAAGGTCAGAGTCGTTGCTAGGGTTCGTCCATTTCTCTCCCAAGAAATTTCAGTCAAAAGTTCTAGGGTTCCAGTTTCATGCATTTCTGTGGTGGATCAAGGGCGTATCTCTCCCGAGGAGGTTACTGTTAATCTCAAAGATCCGGAAACCAGTCGAAACGAGTGCTATCAATTGGACTCATTTTTTGGCCAAGAAGATCACACGGTGAGGCAGATATTCGATAAAGAAGTAAATCCTTTGATTCCTGGACTCTTCCATGGATGTAATGCTACTGTGTTTGCTTACGGGGCTACTGGCAGTGGAAAGTCCTACACCATGCAGGGTACTGAAGAAGAACCAGGTCTAATGCCACTAGCCATGTCTAGAGTTTTATCACTTTGTGAGGAAACTGGGTGTAGAGCAGAAATGTCGTACTATGAAGTCTACTTGGAAAGGTGTCATGATCTTCTGGAACCCAAAGCTAAAGAAATTGTAATTATGGATGATAAAGAAGGGCAAATTCATCTCAGAGGTTTATCTAAGGTTGCTGTAAATTCCATGTCCGAATTCCGGGAGACATTGGCTCTTGGAGTTCAAAGGCGGAAAGTTGCTGATACCGATCTTAACGATGTCTCAAGTAGAAGTCATGGGGTTCTTGTTATTACAGTTTCATCGCCCGTATGTGGTGATTCGGCGGCTTCTGTTACCGGAAAGCTCAACCTCATAGACTTGGCAGGCAATGAAGATAACAGGAGGACTGGCAATGAAGGGATTCGCCTTCAAGAGAGCGCGAAAATCAACCAGTCGTTGTTTGCGCTTTCAAATGTGATATATGCATTAAACAAGAACCTTGCCCGAGTACCTTACAGAGAAAGCAAATTGACCCGCATACTGCAAGATTCTCTCGGTGGATCAAGCCGTGCCTTAATGATTGCTTGCCTGAATCCAGGAGCATATCAGGAGTCTGTCCATACAGTCAGCTTGGCAGCTAGATCGCGTCAAATTTCGAATTTTGTTGTTCCGATCCATAAACAGGGGACTCCACAAGCTAAAGTTGACATGGAATCGAAGCTGCGAGCTTGGCTTGAATCCAAAGGAAAGACCAAAAGTGCACAAAGAATCGGAGGGATTGGTTCTCCATTTTCTAGCAAAACTCCTTGTTCTACTGCATCAAAAGTGAGAAAACCTCTCTTCAATAGTTCTGCCAAAGTTAAGCAAACGGCCGACCAGAGTGACTGTAAACGAGAAAAAGAAAGGACTGTAGCCGTGGCTTTCGGATACTTGGCTGAAAATAAAGATATAGCTAATTCTATCACAACCAAAGAAAAGGTGAAATTCAATAGTCCAAGAAAGGCTCTATCACCAATCCACAACACTAATGGAATCCAAAAGGGTGTGCTGGAACTCTCGTTTACAGACCAATTACCAGCAAGGACTCCAAAAACACCATTCCCAGCAAACGAGGAATTCAAATCTTTTGGGACCCCATTAGATAAGTTTGATGCCCAAGTATCAACCCTCAAGAGTTGCGTTGCACAAGAATATGTGGATTTCTTGAATACAGCAAGCAGAGAGGAACTAGTAGAATTGAAGGGCATTGGAGTGAAAATGGCAGAGTACATTGTGGATCTCAGAGAAACTAGTCCATTGAAATCGCTTGCTGACTTGAAGAAACTGGGACTCTCATCCAAGCAGATCCATAACTTATTCAGCAAAGCTGCAATGGGAATATTTGGTAGAATAGAAGATTTGGCTGCTGTCCCTTGCCCAGAAACTTCATTATCCAACCAATAA

Protein sequence

MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTADQSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPCPETSLSNQ
Homology
BLAST of Cp4.1LG03g02320 vs. ExPASy Swiss-Prot
Match: Q5E913 (Kinesin-like protein KIN-10B OS=Arabidopsis thaliana OX=3702 GN=KIN10B PE=2 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.8e-169
Identity = 348/621 (56.04%), Postives = 422/621 (67.95%), Query Frame = 0

Query: 14  SCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNEC 73
           S +IS VRVV RVRPFL +EIS +S     SC+SV+        EV V LKDP++ RNE 
Sbjct: 15  SSSISNVRVVLRVRPFLPREISDESCD-GRSCVSVIGGDGGDTSEVAVYLKDPDSCRNES 74

Query: 74  YQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPGL 133
           YQLD+F+G+ED  V+ IFD+EV+PLIPG+FHG NATV AYGATGSGK++TMQG +E PGL
Sbjct: 75  YQLDAFYGREDDNVKHIFDREVSPLIPGIFHGFNATVLAYGATGSGKTFTMQGIDELPGL 134

Query: 134 MPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKV 193
           MPL MS +LS+CE+T  RAE+SYYEVY++RC DLLE K  EI + DDK+GQ+HL+GLS V
Sbjct: 135 MPLTMSTILSMCEKTRSRAEISYYEVYMDRCWDLLEVKDNEIAVWDDKDGQVHLKGLSSV 194

Query: 194 AVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLI 253
            V SMSEF+E    GVQRRKVA T LNDVSSRSHGVLVI+V+      S   VTGK+NLI
Sbjct: 195 PVKSMSEFQEAYLCGVQRRKVAHTGLNDVSSRSHGVLVISVT------SQGLVTGKINLI 254

Query: 254 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSL 313
           DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNV+YALN NL RVPYRE+KLTRILQDSL
Sbjct: 255 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVVYALNNNLPRVPYRETKLTRILQDSL 314

Query: 314 GGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWL 373
           GG+SRALM+ACLNPG YQES+ TVSLAARSR ISN V    K  TP+ K+DME+KL+AWL
Sbjct: 315 GGTSRALMVACLNPGEYQESLRTVSLAARSRHISNNVSLNPKVETPKVKIDMEAKLQAWL 374

Query: 374 ESKGKTKSAQRIGGIGSPFSSKTPCS-TASKVRKPLFNSSAKVKQTADQSDCKREKERTV 433
           ESKGK KSA R+  I SP       S + S V+K L + SA  +        +R+   T 
Sbjct: 375 ESKGKMKSAHRMMAIRSPLMGTNQTSISQSSVKKLLCHRSAIAESAKLAGTGQRDAFVTA 434

Query: 434 AVAFGY--LAEN------KDIANSITTKEKVKFNSPRKAL-----SPIHNTNGIQKGVLE 493
              FG   LA +      +++  +  TKE  +  S  + L     S   NT  ++K   E
Sbjct: 435 RNLFGVETLAASHLWEPIRNLQLASPTKEDERDTSGEENLLVSEASLRDNTLDVEKKYTE 494

Query: 494 LS--------------------FTDQLPARTPKTPF-PANEEFKSFGTPLDKFDAQVSTL 553
           LS                     +  L   TPKTPF   N E         KF+A  + L
Sbjct: 495 LSPLREALSPIDSNAKPNSAHGSSPFLKPMTPKTPFLSTNPEIMQMEGTCQKFNAWSNNL 554

Query: 554 KSCVAQEYVDFLNTASREELVELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIH 600
           K+ + +EY+ FLNTA+REEL ELKGIG KMAEYI++LRETSPLKSL DL+K+G +S+Q+H
Sbjct: 555 KTSLIKEYIHFLNTANREELRELKGIGQKMAEYIIELRETSPLKSLTDLEKIGFTSRQVH 614

BLAST of Cp4.1LG03g02320 vs. ExPASy Swiss-Prot
Match: Q6L512 (Kinesin-like protein KIN-10C OS=Oryza sativa subsp. japonica OX=39947 GN=KIN10C PE=2 SV=1)

HSP 1 Score: 553.1 bits (1424), Expect = 3.8e-156
Identity = 335/608 (55.10%), Postives = 411/608 (67.60%), Query Frame = 0

Query: 9   LAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPET 68
           +A A +     VRVV RVRP L  E    S+  P  C+ ++  G     EVTV LKD  T
Sbjct: 1   MATAAATQSQPVRVVLRVRPHLPSE--ANSAEAP--CVGLL--GSHPGGEVTVQLKDQYT 60

Query: 69  SRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTE 128
           SRNECY+LD+FFGQE   V +IFD+EV+ +IPG+F G NATVFAYGATGSGK+YTMQGTE
Sbjct: 61  SRNECYKLDAFFGQESR-VCEIFDQEVSAVIPGIFEGTNATVFAYGATGSGKTYTMQGTE 120

Query: 129 EEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLR 188
           + PGL+PLA+S VL+LC  T C  E+SYYEVY+ERC+DLLEPKA+EI+++DDK+G + L+
Sbjct: 121 DLPGLIPLAVSTVLALCTGTWCSVEISYYEVYMERCYDLLEPKAREIMVLDDKDGNLQLK 180

Query: 189 GLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTG 248
           GL+ V V S+ EF E  ++GVQRRKVA T LNDVSSRSH VL I +++ V       V G
Sbjct: 181 GLAWVPVRSLEEFHEIYSIGVQRRKVAHTGLNDVSSRSHAVLSIRITTDV-------VKG 240

Query: 249 KLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRI 308
           KLNLIDLAGNEDNRRT NEGIRLQESAKINQSLFALSNVI ALNK   R+PYRESKLTRI
Sbjct: 241 KLNLIDLAGNEDNRRTCNEGIRLQESAKINQSLFALSNVISALNKKEPRIPYRESKLTRI 300

Query: 309 LQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESK 368
           LQDSLGG+S A+MIACLNP  YQE+VHTVSLAARSR ++N +    KQ TP+ KVDME+K
Sbjct: 301 LQDSLGGNSHAVMIACLNPVEYQEAVHTVSLAARSRHVTNHMSSASKQETPKDKVDMEAK 360

Query: 369 LRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTA---------------------SKVRKP 428
           LRAWLESKGKTKS QR+ G+ SP + KTP S +                      K++K 
Sbjct: 361 LRAWLESKGKTKSIQRMDGLLSPNAIKTPLSMSHKKQSASGRVSGRGKAMDQDGGKIKKV 420

Query: 429 LFNSSAKVKQTADQSDCKREKERTVAVAFGYLAENKDIANSIT-TKEKVKFNSPRKALSP 488
           LF+S+A++       +  ++   T           K +  S+T  KE    +S RKALSP
Sbjct: 421 LFDSAARIPAENFHREGTQDIVNTT---------KKVVLPSLTPCKEDKTGSSLRKALSP 480

Query: 489 IHNTNGIQK--GVLELSFTDQLPARTPKTPFPANEEFKSFG-TPLDKFDAQVSTLKSCVA 548
           I +    QK     + +    L  RTP      N   K  G TPLDKF A  S LK  + 
Sbjct: 481 ISSNMDPQKQRTADDSNCLMLLELRTPMG--SCNIVGKVTGATPLDKFIALGSNLKESLI 540

Query: 549 QEYVDFLNTASREELVELKGIGVKMAEYIVDLRETS--PLKSLADLKKLGLSSKQIHNLF 590
           Q+Y+DFLN A++EEL +LKGIG + AEYI++LRE S  P KSL+DL  +GLSSKQI ++ 
Sbjct: 541 QQYLDFLNVANKEELQKLKGIGERRAEYILELREDSPRPFKSLSDLGNIGLSSKQIQDIL 583

BLAST of Cp4.1LG03g02320 vs. ExPASy Swiss-Prot
Match: Q6P3R1 (Kinesin-like protein KIF22 OS=Xenopus tropicalis OX=8364 GN=kif22 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 4.3e-67
Identity = 217/646 (33.59%), Postives = 319/646 (49.38%), Query Frame = 0

Query: 18  SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLD 77
           ++VRV  R+RP++ +E      + P +C+  +D   +   E+       ET +   YQ D
Sbjct: 17  ARVRVAVRLRPYMEKE----DEKAPAACVRGLDSQSL---EIVNWRNQLETMQ---YQFD 76

Query: 78  SFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPGLMPLA 137
           +F+G +  T R+I+   V  ++P L  G NA+VFAYG TG+GK++TM G   +PG++P A
Sbjct: 77  AFYG-DSATQREIYMGSVCHILPHLLIGQNASVFAYGPTGAGKTHTMLGNPSQPGVIPRA 136

Query: 138 MSRVLSLCEETGCRAE---------MSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLR 197
           +  +L +        E         MSY E+Y E+  DLLEPK K++ I +DK+  I + 
Sbjct: 137 VRDLLQMTRTAAGGPENENWTYTITMSYVEIYQEKVMDLLEPKNKDLPIREDKDHNILIP 196

Query: 198 GLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTG 257
           G+++  +NS  +F E      Q R VA T LND SSRSH VL+I V           +TG
Sbjct: 197 GVTQKTINSFGDFDEHFIPASQNRTVASTKLNDRSSRSHAVLLIKVQKSQQVSPFRQLTG 256

Query: 258 KLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRI 317
           KL LIDLAG+EDNRRTGN+GIRL+ES  IN SLF LS V+ ALN+ L R+PYR+SKLTR+
Sbjct: 257 KLYLIDLAGSEDNRRTGNQGIRLKESGAINSSLFTLSKVVDALNQGLPRIPYRDSKLTRL 316

Query: 318 LQDSLGGSSRALMIACLNPGA--YQESVHTVSLAARSRQISN-----------FVVPIHK 377
           LQDSLGG++ ++MIA + P    Y +++  ++ AA+S+QI N             +P  K
Sbjct: 317 LQDSLGGTAHSVMIANIAPEQKYYFDTLTALNFAAKSKQIINKPFSQETTQSIAALPAMK 376

Query: 378 QGTPQAKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTAS----------KVR 437
           +   +A+    S+ R     K KT S +         +SK   + A+          K+ 
Sbjct: 377 RPREEAETAAGSRQR----KKSKTDSTESSPNTSMDAASKRKLNLAALDPAVVERLLKLD 436

Query: 438 KPLFNSSAKVKQTADQSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKAL- 497
           K L     K  Q    S  KRE+   +A+   +     +I      KEK K    +KA+ 
Sbjct: 437 KILTEKGMKEAQLL--STPKRER---MALLKKWEESQMEIER---LKEKQK-ELEQKAIE 496

Query: 498 -------SPIHNTNGIQKGVLELSFTDQLPARTPKT----------PFPANEEFKSF--- 557
                  S   + N     V E +F   L  R   T          P   N + +S    
Sbjct: 497 AEARLEKSTNSDCNLSDSSVSECTFRAPLRGRNTSTAKAKKVLRVLPMQGNSQLQSTIEE 556

Query: 558 GTPLDKFDAQVSTLKSCVAQE--------------------YVDFLNTASREELVELKGI 589
           G P+  F+ +     SC  +E                     +  LNT S +EL  L+ I
Sbjct: 557 GIPV--FEKKKKKPVSCDGRENQPTWEVNVRTDLLESGRERILKLLNTGSVKELKSLQKI 616

BLAST of Cp4.1LG03g02320 vs. ExPASy Swiss-Prot
Match: Q3V300 (Kinesin-like protein KIF22 OS=Mus musculus OX=10090 GN=Kif22 PE=2 SV=2)

HSP 1 Score: 254.6 bits (649), Expect = 2.8e-66
Identity = 206/640 (32.19%), Postives = 321/640 (50.16%), Query Frame = 0

Query: 17  ISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQL 76
           +++VRV  R+RPF+  E   K     + C+  +D   +   EV    K  ET +   YQ 
Sbjct: 36  LARVRVAVRLRPFMDGETEAKE----LPCVRAIDSCSL---EVANWKKYQETLK---YQF 95

Query: 77  DSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPGLMPL 136
           D+F+G E  T ++++   V P++  L  G NA+V AYG TG+GK++TM G+ E+PG++P 
Sbjct: 96  DAFYG-EKSTQQEVYVGSVQPILRHLLEGQNASVLAYGPTGAGKTHTMLGSPEQPGVIPR 155

Query: 137 AMSRVLSLCEETGCRAE-------MSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRG 196
           A+  +L L  E             MSY E+Y E+  DLL+P + ++VI +D  G I + G
Sbjct: 156 ALMDLLQLAREESAEGRPWDVSVAMSYLEIYQEKVLDLLDPASGDLVIREDCRGNILIPG 215

Query: 197 LSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGK 256
           L++  + S S+F +      + R V  T LN  SSRSH VL++ V             GK
Sbjct: 216 LTQKPITSFSDFEQHFLPASRNRAVGATRLNQRSSRSHAVLLVKVDQRERLTPFRQREGK 275

Query: 257 LNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRIL 316
           L LIDLAG+EDNRRTGN+GIRL+ES  IN SLF L  V+ ALN+ L R+PYR+SKLTR+L
Sbjct: 276 LYLIDLAGSEDNRRTGNQGIRLKESGAINTSLFVLGKVVDALNQGLPRIPYRDSKLTRLL 335

Query: 317 QDSLGGSSRALMIACLNPGA--YQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMES 376
           QDSLGGS+ +++IA + P    YQ+++  ++  ARS+++ N     ++   P A   ++ 
Sbjct: 336 QDSLGGSAHSILIANIAPERRFYQDTISALNFTARSKEVINRPF-TNESLQPHALAPVKL 395

Query: 377 KLRAWL-ESKGKTKSAQRIGGIGSPFSSKTPCSTASKVR--------------------- 436
             +  L  S+ K          GSP S+  P S + K+                      
Sbjct: 396 SQKELLGPSEAKKAKGPEEESTGSPESTAAPASASQKLSLLQKLSNMDPAMLENLLSMER 455

Query: 437 ---------KPLFNSSAK----VKQTADQSDCKREKERTVAVAFGYLAENKDIANSITTK 496
                     PL N+  +    + +T ++ + + E+ +          + K++   +  +
Sbjct: 456 LLGSQGSQGTPLLNTPKRERMVLMKTVEEKNLEIERLK---------MKQKELEAKVLAQ 515

Query: 497 E----KVKFNSPRKALSPIHNTNG-----IQKGVL----------ELSFTDQLPARTPKT 556
           E    + K N+P   L P  + +G     ++K V+          E S   QL  + PK 
Sbjct: 516 EAPDPREKENTP-TILQPPASYSGTVAKPLKKAVVMPLQRIQKQRESSNQIQLLKKGPKR 575

Query: 557 PFPANEEFKSFGTPLDKFDAQVS-TLKSCVAQEYVDFLNTASREELVELKGIGVKMAEYI 586
               + E ++     D ++ Q+S  L +   ++ +D LN  S  EL  L+ IG K A+ I
Sbjct: 576 KLEPSPESEAVEKDEDYWEVQISPELLAHGRKKLLDLLNEGSARELRSLQRIGQKKAQLI 635

BLAST of Cp4.1LG03g02320 vs. ExPASy Swiss-Prot
Match: Q7ZYL5 (Kinesin-like protein KIF22-B OS=Xenopus laevis OX=8355 GN=kif22-b PE=2 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 8.2e-66
Identity = 214/638 (33.54%), Postives = 324/638 (50.78%), Query Frame = 0

Query: 13  TSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNE 72
           +SCA  +VRV  R+RP++ ++   +  +VP +C+  +D   +   E+       ET +  
Sbjct: 27  SSCA--RVRVAVRLRPYMEEK---EEDKVPTACVRGLDSHSL---EIVNWRNQLETMQ-- 86

Query: 73  CYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPG 132
            YQ D+F+G +  + R+I+   V  ++P L  G NA+VFAYG TG+GK++TM G  ++PG
Sbjct: 87  -YQFDAFYG-DSASQREIYMGSVCHILPHLLIGQNASVFAYGPTGAGKTHTMLGNPDQPG 146

Query: 133 LMPLAMSRVLSLCEETGCRAE---------MSYYEVYLERCHDLLEPKAKEIVIMDDKEG 192
           ++P A+  +L +        E         MSY E+Y E+  DLLEPK K++ I +DK+ 
Sbjct: 147 VIPRAVRELLQMTRMAASAPENENWTYTITMSYVEIYQEKVMDLLEPKNKDLPIREDKDH 206

Query: 193 QIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSA 252
            I + G++   +NS  +F E      Q R VA T LND SSRSH VL+I V         
Sbjct: 207 NILIPGVTLKTINSFGDFDEHFIPASQNRTVASTKLNDRSSRSHAVLLIKVQKSQQVAPF 266

Query: 253 ASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRES 312
             + GKL LIDLAG+EDNRRTGN+GIRL+ES  IN SLF LS V+ ALN+ L R+PYR+S
Sbjct: 267 RQLIGKLYLIDLAGSEDNRRTGNQGIRLKESGAINSSLFTLSKVVDALNQGLPRIPYRDS 326

Query: 313 KLTRILQDSLGGSSRALMIACLNPGA--YQESVHTVSLAARSRQISN----------FVV 372
           KLTR+LQDSLGGS+ ++MI  + P    Y +++  ++ AA+S+QI N             
Sbjct: 327 KLTRLLQDSLGGSAHSVMITNIAPEQTYYFDTLTALNFAAKSKQIINKPFSRETTQTVAQ 386

Query: 373 PIHKQGTPQAKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTAS--------- 432
           P  K+   +A+    S+ R     K KT S +         + K   + AS         
Sbjct: 387 PAMKRPREEAEATTSSRQR----KKSKTDSTESSPNSSMESTGKRKLNLASLDSAVVERL 446

Query: 433 -KVRKPLFNSSAKVKQ----------------TADQSDCKREKER--------TVAVAFG 492
            K+ K L     K  Q                   Q + +R KE+          A A  
Sbjct: 447 LKLDKILTEKGKKEAQLLSTPKRERMALLKKWEESQMEIERLKEKQKELEQKAMEAEARL 506

Query: 493 YLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQK--GVLELSFTDQLPARTPKTPF 552
             + N D+++S  ++    F +P +  +   +T  ++K   VL +    QL + T +   
Sbjct: 507 EKSNNSDLSDSSVSEN--TFRAPLRGRNT--STAKVKKVLRVLPMQGNSQLQS-TIEEGI 566

Query: 553 PANEEFKSFGTPLDKFDAQV-------STLKSCVAQEYVDFLNTASREELVELKGIGVKM 585
           P  E+ K      D  + Q        + L     +  +  LNT S +EL  L+ IG K 
Sbjct: 567 PVFEKKKKKQVTCDGHENQPTWEMNMRTDLLESGKERILKLLNTGSVKELKSLQRIGDKK 626

BLAST of Cp4.1LG03g02320 vs. NCBI nr
Match: XP_023528172.1 (kinesin-like protein KIN-10B [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1181 bits (3055), Expect = 0.0
Identity = 608/608 (100.00%), Postives = 608/608 (100.00%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480
           QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL
Sbjct: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480

Query: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540
           SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE
Sbjct: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540

Query: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600
           LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC
Sbjct: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600

Query: 601 PETSLSNQ 608
           PETSLSNQ
Sbjct: 601 PETSLSNQ 608

BLAST of Cp4.1LG03g02320 vs. NCBI nr
Match: XP_022934838.1 (kinesin-like protein KIN-10B isoform X3 [Cucurbita moschata])

HSP 1 Score: 1166 bits (3016), Expect = 0.0
Identity = 599/608 (98.52%), Postives = 604/608 (99.34%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480
           QSDCKREKERTVAVAFG L ENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480

Query: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540
           SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE
Sbjct: 481 SFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540

Query: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600
           LKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC
Sbjct: 541 LKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600

Query: 601 PETSLSNQ 608
           PETSLSNQ
Sbjct: 601 PETSLSNQ 608

BLAST of Cp4.1LG03g02320 vs. NCBI nr
Match: XP_022983426.1 (kinesin-like protein KIN-10B isoform X3 [Cucurbita maxima])

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 598/608 (98.36%), Postives = 603/608 (99.18%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480
           QSDCKREKERTVAVAFG L ENKDIANSI TKEKVKFNSPRKALSPIHNTNGIQK VLE+
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKEKVKFNSPRKALSPIHNTNGIQKAVLEI 480

Query: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540
           SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE
Sbjct: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540

Query: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600
           LKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC
Sbjct: 541 LKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600

Query: 601 PETSLSNQ 608
           PETSLSNQ
Sbjct: 601 PETSLSNQ 608

BLAST of Cp4.1LG03g02320 vs. NCBI nr
Match: XP_022934836.1 (kinesin-like protein KIN-10B isoform X2 [Cucurbita moschata])

HSP 1 Score: 1161 bits (3003), Expect = 0.0
Identity = 599/610 (98.20%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTK--EKVKFNSPRKALSPIHNTNGIQKGVL 480
           QSDCKREKERTVAVAFG L ENKDIANSITTK  EKVKFNSPRKALSPIHNTNGIQKGVL
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHEKVKFNSPRKALSPIHNTNGIQKGVL 480

Query: 481 ELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540
           ELSF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL
Sbjct: 481 ELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540

Query: 541 VELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600
           VELKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV
Sbjct: 541 VELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600

Query: 601 PCPETSLSNQ 608
           PCPETSLSNQ
Sbjct: 601 PCPETSLSNQ 610

BLAST of Cp4.1LG03g02320 vs. NCBI nr
Match: XP_022934835.1 (kinesin-like protein KIN-10B isoform X1 [Cucurbita moschata])

HSP 1 Score: 1160 bits (3002), Expect = 0.0
Identity = 599/611 (98.04%), Postives = 604/611 (98.85%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTK---EKVKFNSPRKALSPIHNTNGIQKGV 480
           QSDCKREKERTVAVAFG L ENKDIANSITTK   EKVKFNSPRKALSPIHNTNGIQKGV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHVEKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LELSF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 608
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of Cp4.1LG03g02320 vs. ExPASy TrEMBL
Match: A0A6J1F8V6 (Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1)

HSP 1 Score: 1166 bits (3016), Expect = 0.0
Identity = 599/608 (98.52%), Postives = 604/608 (99.34%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480
           QSDCKREKERTVAVAFG L ENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480

Query: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540
           SF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE
Sbjct: 481 SFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540

Query: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600
           LKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC
Sbjct: 541 LKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600

Query: 601 PETSLSNQ 608
           PETSLSNQ
Sbjct: 601 PETSLSNQ 608

BLAST of Cp4.1LG03g02320 vs. ExPASy TrEMBL
Match: A0A6J1J264 (Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1)

HSP 1 Score: 1162 bits (3006), Expect = 0.0
Identity = 598/608 (98.36%), Postives = 603/608 (99.18%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTKEKVKFNSPRKALSPIHNTNGIQKGVLEL 480
           QSDCKREKERTVAVAFG L ENKDIANSI TKEKVKFNSPRKALSPIHNTNGIQK VLE+
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKEKVKFNSPRKALSPIHNTNGIQKAVLEI 480

Query: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540
           SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE
Sbjct: 481 SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREELVE 540

Query: 541 LKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600
           LKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC
Sbjct: 541 LKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAVPC 600

Query: 601 PETSLSNQ 608
           PETSLSNQ
Sbjct: 601 PETSLSNQ 608

BLAST of Cp4.1LG03g02320 vs. ExPASy TrEMBL
Match: A0A6J1F2Y6 (Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1)

HSP 1 Score: 1161 bits (3003), Expect = 0.0
Identity = 599/610 (98.20%), Postives = 604/610 (99.02%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTK--EKVKFNSPRKALSPIHNTNGIQKGVL 480
           QSDCKREKERTVAVAFG L ENKDIANSITTK  EKVKFNSPRKALSPIHNTNGIQKGVL
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHEKVKFNSPRKALSPIHNTNGIQKGVL 480

Query: 481 ELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540
           ELSF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL
Sbjct: 481 ELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540

Query: 541 VELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600
           VELKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV
Sbjct: 541 VELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600

Query: 601 PCPETSLSNQ 608
           PCPETSLSNQ
Sbjct: 601 PCPETSLSNQ 610

BLAST of Cp4.1LG03g02320 vs. ExPASy TrEMBL
Match: A0A6J1F8V4 (Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1)

HSP 1 Score: 1160 bits (3002), Expect = 0.0
Identity = 599/611 (98.04%), Postives = 604/611 (98.85%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCA+SKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQG ISPEEVT
Sbjct: 1   MAAENFELLAPATSCAVSKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGHISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTK---EKVKFNSPRKALSPIHNTNGIQKGV 480
           QSDCKREKERTVAVAFG L ENKDIANSITTK   EKVKFNSPRKALSPIHNTNGIQKGV
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSITTKDHVEKVKFNSPRKALSPIHNTNGIQKGV 480

Query: 481 LELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540
           LELSF DQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE
Sbjct: 481 LELSFADQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREE 540

Query: 541 LVELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600
           LVELKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA
Sbjct: 541 LVELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAA 600

Query: 601 VPCPETSLSNQ 608
           VPCPETSLSNQ
Sbjct: 601 VPCPETSLSNQ 611

BLAST of Cp4.1LG03g02320 vs. ExPASy TrEMBL
Match: A0A6J1J5U2 (Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1)

HSP 1 Score: 1157 bits (2993), Expect = 0.0
Identity = 598/610 (98.03%), Postives = 603/610 (98.85%), Query Frame = 0

Query: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60
           MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT
Sbjct: 1   MAAENFELLAPATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVT 60

Query: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGK 120
           VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLF GCNATVFAYGATGSGK
Sbjct: 61  VNLKDPETSRNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFQGCNATVFAYGATGSGK 120

Query: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180
           SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD
Sbjct: 121 SYTMQGTEEEPGLMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDD 180

Query: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240
           KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG
Sbjct: 181 KEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCG 240

Query: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300
           DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY
Sbjct: 241 DSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPY 300

Query: 301 RESKLTRILQDSLGGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360
           RESKLTRILQDSLGG+SRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ
Sbjct: 301 RESKLTRILQDSLGGTSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQ 360

Query: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKQTAD 420
           AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVK+TA+
Sbjct: 361 AKVDMESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTASKVRKPLFNSSAKVKKTAN 420

Query: 421 QSDCKREKERTVAVAFGYLAENKDIANSITTK--EKVKFNSPRKALSPIHNTNGIQKGVL 480
           QSDCKREKERTVAVAFG L ENKDIANSI TK  EKVKFNSPRKALSPIHNTNGIQK VL
Sbjct: 421 QSDCKREKERTVAVAFGNLVENKDIANSIATKDHEKVKFNSPRKALSPIHNTNGIQKAVL 480

Query: 481 ELSFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540
           E+SFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL
Sbjct: 481 EISFTDQLPARTPKTPFPANEEFKSFGTPLDKFDAQVSTLKSCVAQEYVDFLNTASREEL 540

Query: 541 VELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600
           VELKGIGVKMAEYI+DLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV
Sbjct: 541 VELKGIGVKMAEYILDLRETSPLKSLADLKKLGLSSKQIHNLFSKAAMGIFGRIEDLAAV 600

Query: 601 PCPETSLSNQ 608
           PCPETSLSNQ
Sbjct: 601 PCPETSLSNQ 610

BLAST of Cp4.1LG03g02320 vs. TAIR 10
Match: AT5G02370.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 595.1 bits (1533), Expect = 6.3e-170
Identity = 348/621 (56.04%), Postives = 422/621 (67.95%), Query Frame = 0

Query: 14  SCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNEC 73
           S +IS VRVV RVRPFL +EIS +S     SC+SV+        EV V LKDP++ RNE 
Sbjct: 15  SSSISNVRVVLRVRPFLPREISDESCD-GRSCVSVIGGDGGDTSEVAVYLKDPDSCRNES 74

Query: 74  YQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPGL 133
           YQLD+F+G+ED  V+ IFD+EV+PLIPG+FHG NATV AYGATGSGK++TMQG +E PGL
Sbjct: 75  YQLDAFYGREDDNVKHIFDREVSPLIPGIFHGFNATVLAYGATGSGKTFTMQGIDELPGL 134

Query: 134 MPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSKV 193
           MPL MS +LS+CE+T  RAE+SYYEVY++RC DLLE K  EI + DDK+GQ+HL+GLS V
Sbjct: 135 MPLTMSTILSMCEKTRSRAEISYYEVYMDRCWDLLEVKDNEIAVWDDKDGQVHLKGLSSV 194

Query: 194 AVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNLI 253
            V SMSEF+E    GVQRRKVA T LNDVSSRSHGVLVI+V+      S   VTGK+NLI
Sbjct: 195 PVKSMSEFQEAYLCGVQRRKVAHTGLNDVSSRSHGVLVISVT------SQGLVTGKINLI 254

Query: 254 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDSL 313
           DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNV+YALN NL RVPYRE+KLTRILQDSL
Sbjct: 255 DLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVVYALNNNLPRVPYRETKLTRILQDSL 314

Query: 314 GGSSRALMIACLNPGAYQESVHTVSLAARSRQISNFVVPIHKQGTPQAKVDMESKLRAWL 373
           GG+SRALM+ACLNPG YQES+ TVSLAARSR ISN V    K  TP+ K+DME+KL+AWL
Sbjct: 315 GGTSRALMVACLNPGEYQESLRTVSLAARSRHISNNVSLNPKVETPKVKIDMEAKLQAWL 374

Query: 374 ESKGKTKSAQRIGGIGSPFSSKTPCS-TASKVRKPLFNSSAKVKQTADQSDCKREKERTV 433
           ESKGK KSA R+  I SP       S + S V+K L + SA  +        +R+   T 
Sbjct: 375 ESKGKMKSAHRMMAIRSPLMGTNQTSISQSSVKKLLCHRSAIAESAKLAGTGQRDAFVTA 434

Query: 434 AVAFGY--LAEN------KDIANSITTKEKVKFNSPRKAL-----SPIHNTNGIQKGVLE 493
              FG   LA +      +++  +  TKE  +  S  + L     S   NT  ++K   E
Sbjct: 435 RNLFGVETLAASHLWEPIRNLQLASPTKEDERDTSGEENLLVSEASLRDNTLDVEKKYTE 494

Query: 494 LS--------------------FTDQLPARTPKTPF-PANEEFKSFGTPLDKFDAQVSTL 553
           LS                     +  L   TPKTPF   N E         KF+A  + L
Sbjct: 495 LSPLREALSPIDSNAKPNSAHGSSPFLKPMTPKTPFLSTNPEIMQMEGTCQKFNAWSNNL 554

Query: 554 KSCVAQEYVDFLNTASREELVELKGIGVKMAEYIVDLRETSPLKSLADLKKLGLSSKQIH 600
           K+ + +EY+ FLNTA+REEL ELKGIG KMAEYI++LRETSPLKSL DL+K+G +S+Q+H
Sbjct: 555 KTSLIKEYIHFLNTANREELRELKGIGQKMAEYIIELRETSPLKSLTDLEKIGFTSRQVH 614

BLAST of Cp4.1LG03g02320 vs. TAIR 10
Match: AT5G23910.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 223.8 bits (569), Expect = 3.8e-58
Identity = 220/721 (30.51%), Postives = 336/721 (46.60%), Query Frame = 0

Query: 13  TSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNE 72
           TS     VRVVARV+P  S +++   S      ISV        E VT++         +
Sbjct: 2   TSHGRKVVRVVARVKP--STDLASTKS------ISVQKPMGDDSETVTISFGAQFAGSKD 61

Query: 73  CYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEEEPG 132
            Y+LD  + +E+ T   I  KE+ PLI  +F G +A V A+GA  SGK++ +QG E E G
Sbjct: 62  SYRLDYCY-EENETTGSILTKEIKPLISTVFEGKDANVIAHGARNSGKTHLIQGNERELG 121

Query: 133 LMPLAMSRVLSLCEETGCRAEMSYYEVYLERCHDLLEPKAKEIVIMDDKEGQIHLRGLSK 192
           L  L MS +LS+ EE G    +S YEV  E  +DLL+ + + + +++  +G+I L+GLS+
Sbjct: 122 LAVLTMSEMLSMAEERGDAIFVSVYEVSQETVYDLLDQEKRVVSVLEGAQGKIQLKGLSQ 181

Query: 193 VAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAASVTGKLNL 252
           V V S+SEF + L  G ++ +   +DL    +RSH  ++I V++   G++ +   G++N 
Sbjct: 182 VPVKSLSEF-QNLYFGFKKSQKLTSDL---PTRSHKGVMIHVTT---GNANSGSLGRMNF 241

Query: 253 IDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLARVPYRESKLTRILQDS 312
           +D+AG ED+R+  N  +   E A++N+S++AL NV+YALN N + VPYRESKLTR+L+D 
Sbjct: 242 LDMAGYEDSRKQ-NSALGPLEIARVNKSIYALQNVMYALNANESHVPYRESKLTRMLKDC 301

Query: 313 LGGSSRALMIACLNPGAYQESVHTVSLAAR-----SRQISNFVVPIHKQ--GTPQAKVDM 372
           L GS+  L+I CL     Q+S + ++LA+R     +R I+N   P  K+  G  ++    
Sbjct: 302 LKGSNITLLITCLPREFSQDSFYMLNLASRICLGGNRAITN---PTKKKINGLDRSVSLS 361

Query: 373 ESKLRAWLESKGKTKSAQRIGGIGSPFSSKTPCSTAS---KVRKPLF----------NSS 432
            +  R          S ++    G+    KT  +TA+   K RK LF          NSS
Sbjct: 362 SAAQRRQTPLTMSAASRKQTVLRGNVTERKTKINTATSAIKARK-LFGEANDSVKCKNSS 421

Query: 433 AKVKQTADQSDCKREKERTVAVAFGYLAENKDIANSITTKE----------KVKFNSPRK 492
            KV+  A     K      V ++    +  ++I +SIT  +           + F+S   
Sbjct: 422 KKVEGKAKMVLKKGISTSKVVLSVQASSPKEEICSSITVTDFQSSLVEEEYSLAFSSSTV 481

Query: 493 ALSP------------IHNTNG-------------------------------------- 552
           A+ P            I+ T+                                       
Sbjct: 482 AMEPGYSNGASLSSEAIYTTDKETPRKQEEMFAGATHCDDAFVDKAQIVERDENNSVIEE 541

Query: 553 -----IQKG-----------------------VLELS--------FTDQLPA----RTPK 590
                I +G                       + ELS        F++QL      +TP 
Sbjct: 542 DLTLVIDEGENLDKENNSLLANETASPPLSMRLQELSNNLKSICKFSNQLSVPEKHQTPL 601

BLAST of Cp4.1LG03g02320 vs. TAIR 10
Match: AT3G49650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 193.7 bits (491), Expect = 4.2e-49
Identity = 132/351 (37.61%), Postives = 194/351 (55.27%), Query Frame = 0

Query: 10  APATSCAISKVRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETS 69
           APA     + + V  + RP + +E      RV  S   VV    +S + +    +    +
Sbjct: 6   APAAK-KTTTLTVAVKCRPLMEKERGRDIVRVNNSKEVVVLDPDLSKDYLD---RIQNRT 65

Query: 70  RNECYQLDSFFGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQGTEE 129
           + + Y  D  FG E  T + ++ + ++ +I  + HG NATVFAYG+TGSGK+YTM GT  
Sbjct: 66  KEKKYCFDHAFGPES-TNKNVY-RSMSSVISSVVHGLNATVFAYGSTGSGKTYTMVGTRS 125

Query: 130 EPGLMPLAMSRVLSLCEETGCRAEM----SYYEVYLERCHDLLEPKAKEIVIMDDKEGQI 189
           +PGLM L+++ +  + +      E     SY EVY E  +DLLE  +  + + +D E  I
Sbjct: 126 DPGLMVLSLNTIFDMIKSDKSSDEFEVTCSYLEVYNEVIYDLLEKSSGHLELREDPEQGI 185

Query: 190 HLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITVSSPVCGDSAAS 249
            + GL  + V+S     E L LG  RRK   T++N  SSRSH VL I V       +   
Sbjct: 186 VVAGLRSIKVHSADRILELLNLGNSRRKTESTEMNGTSSRSHAVLEIAVKRRQKNQNQV- 245

Query: 250 VTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYAL----NKNLARVPYR 309
           + GKL L+DLAG+E    T N G +L++ A IN+SL AL+N I AL     K LA VPYR
Sbjct: 246 MRGKLALVDLAGSERAAETNNGGQKLRDGANINRSLLALANCINALGKQHKKGLAYVPYR 305

Query: 310 ESKLTRILQDSLGGSSRALMIACLNP--GAYQESVHTVSLAARSRQISNFV 351
            SKLTRIL+D L G+S+ +M+A ++P    Y  +V+T+  A R+++I   +
Sbjct: 306 NSKLTRILKDGLSGNSQTVMVATISPADSQYHHTVNTLKYADRAKEIKTHI 349

BLAST of Cp4.1LG03g02320 vs. TAIR 10
Match: AT2G37420.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 181.0 bits (458), Expect = 2.8e-45
Identity = 124/353 (35.13%), Postives = 187/353 (52.97%), Query Frame = 0

Query: 20  VRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSF 79
           V+V+ R +P   +E      RV +SC  +         EV V         +  +  D  
Sbjct: 49  VQVILRCKPLSEEEQKSSVPRV-ISCNEM-------RREVNVLHTIANKQVDRLFNFDKV 108

Query: 80  FGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTMQG--------TEEEP 139
           FG +    R I+D+ + P++  +  G + TVFAYG TG+GK+YTM+G           E 
Sbjct: 109 FGPKSQQ-RSIYDQAIAPIVHEVLEGFSCTVFAYGQTGTGKTYTMEGGMRKKGGDLPAEA 168

Query: 140 GLMPLAMSRVLSLCEETGCRAEM--SYYEVYLERCHDLL----------EPKAKEIVIMD 199
           G++P A+  +    E       M  ++ E+Y E   DLL          + + K I +M+
Sbjct: 169 GVIPRAVRHIFDTLEAQNADYSMKVTFLELYNEEVTDLLAQDDSSRSSEDKQRKPISLME 228

Query: 200 DKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVITV--SSP 259
           D +G + LRGL +  V S ++    L  G  +R+ ADT LN  SSRSH V  ITV     
Sbjct: 229 DGKGSVVLRGLEEEVVYSANDIYALLERGSSKRRTADTLLNKRSSRSHSVFTITVHIKEE 288

Query: 260 VCGDSAASVTGKLNLIDLAGNEDNRRTGNEGIRLQESAKINQSLFALSNVIYALNKNLAR 319
             GD      GKLNL+DLAG+E+  R+G    R +E+ +IN+SL  L  VI AL ++ + 
Sbjct: 289 SMGDEELIKCGKLNLVDLAGSENILRSGARDGRAREAGEINKSLLTLGRVINALVEHSSH 348

Query: 320 VPYRESKLTRILQDSLGGSSRALMIACLNPGAY--QESVHTVSLAARSRQISN 349
           VPYR+SKLTR+L+DSLGG ++  +IA ++P A+  +E++ T+  A R++ I N
Sbjct: 349 VPYRDSKLTRLLRDSLGGKTKTCIIATISPSAHSLEETLSTLDYAYRAKNIKN 392

BLAST of Cp4.1LG03g02320 vs. TAIR 10
Match: AT3G50240.1 (ATP binding microtubule motor family protein )

HSP 1 Score: 178.7 bits (452), Expect = 1.4e-44
Identity = 132/375 (35.20%), Postives = 189/375 (50.40%), Query Frame = 0

Query: 20  VRVVARVRPFLSQEISVKSSRVPVSCISVVDQGRISPEEVTVNLKDPETSRNECYQLDSF 79
           V+V   VRP +  E++ +  R  VS   V  Q ++     T                D  
Sbjct: 26  VKVAVNVRPLIGDEVT-QGCRECVSVSPVTPQVQMGTHPFT---------------FDHV 85

Query: 80  FGQEDHTVRQIFDKEVNPLIPGLFHGCNATVFAYGATGSGKSYTM-----QGTEEEPGLM 139
           +G        +F++ V PL+ GLFHG NATV AYG TGSGK+YTM      GT+   GL+
Sbjct: 86  YGSNGSPSSLMFEECVAPLVDGLFHGYNATVLAYGQTGSGKTYTMGTGIKDGTKN--GLI 145

Query: 140 PLAMS----RVLSLCEETGCRAEMSYYEVYLERCHDLLEPKA-----------------K 199
           P  MS    ++ S+  + G +  +S+ E+  E   DLL+                     
Sbjct: 146 PQVMSALFNKIDSVKHQMGFQLHVSFIEILKEEVLDLLDSSVPFNRLANGTPGKVVLSKS 205

Query: 200 EIVIMDDKEGQIHLRGLSKVAVNSMSEFRETLALGVQRRKVADTDLNDVSSRSHGVLVIT 259
            + I +   G I L G ++V + +  E    L  G   R    T++N+ SSRSH +  IT
Sbjct: 206 PVQIRESPNGVITLSGATEVPIATKEEMASCLEQGSLTRATGSTNMNNESSRSHAIFTIT 265

Query: 260 V-------SSPVCGDSAASVTG------KLNLIDLAGNEDNRRTGNEGIRLQESAKINQS 319
           +       S  V  D+     G      KL+L+DLAG+E  +RTG+ G+RL+E   IN+ 
Sbjct: 266 LEQMRKISSISVVKDTVDEDMGEEYCCAKLHLVDLAGSERAKRTGSGGVRLKEGIHINRG 325

Query: 320 LFALSNVIYAL-----NKNLARVPYRESKLTRILQDSLGGSSRALMIACLNPGAY--QES 349
           L AL NVI AL      K  A VPYR+SKLTR+LQDSLGG+S+ +MIAC++P     +E+
Sbjct: 326 LLALGNVISALGDEKRRKEGAHVPYRDSKLTRLLQDSLGGNSKTVMIACISPADINAEET 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5E9138.8e-16956.04Kinesin-like protein KIN-10B OS=Arabidopsis thaliana OX=3702 GN=KIN10B PE=2 SV=1[more]
Q6L5123.8e-15655.10Kinesin-like protein KIN-10C OS=Oryza sativa subsp. japonica OX=39947 GN=KIN10C ... [more]
Q6P3R14.3e-6733.59Kinesin-like protein KIF22 OS=Xenopus tropicalis OX=8364 GN=kif22 PE=2 SV=1[more]
Q3V3002.8e-6632.19Kinesin-like protein KIF22 OS=Mus musculus OX=10090 GN=Kif22 PE=2 SV=2[more]
Q7ZYL58.2e-6633.54Kinesin-like protein KIF22-B OS=Xenopus laevis OX=8355 GN=kif22-b PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_023528172.10.0100.00kinesin-like protein KIN-10B [Cucurbita pepo subsp. pepo][more]
XP_022934838.10.098.52kinesin-like protein KIN-10B isoform X3 [Cucurbita moschata][more]
XP_022983426.10.098.36kinesin-like protein KIN-10B isoform X3 [Cucurbita maxima][more]
XP_022934836.10.098.20kinesin-like protein KIN-10B isoform X2 [Cucurbita moschata][more]
XP_022934835.10.098.04kinesin-like protein KIN-10B isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1F8V60.098.52Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1[more]
A0A6J1J2640.098.36Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1[more]
A0A6J1F2Y60.098.20Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1[more]
A0A6J1F8V40.098.04Kinesin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111441883 PE=3 SV=1[more]
A0A6J1J5U20.098.03Kinesin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111482032 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G02370.16.3e-17056.04ATP binding microtubule motor family protein [more]
AT5G23910.13.8e-5830.51ATP binding microtubule motor family protein [more]
AT3G49650.14.2e-4937.61P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G37420.12.8e-4535.13ATP binding microtubule motor family protein [more]
AT3G50240.11.4e-4435.20ATP binding microtubule motor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001752Kinesin motor domainPRINTSPR00380KINESINHEAVYcoord: 249..267
score: 48.86
coord: 298..319
score: 62.01
coord: 217..234
score: 50.0
coord: 105..126
score: 65.95
IPR001752Kinesin motor domainSMARTSM00129kinesin_4coord: 17..355
e-value: 6.2E-100
score: 348.0
IPR001752Kinesin motor domainPFAMPF00225Kinesincoord: 25..346
e-value: 3.2E-87
score: 292.6
IPR001752Kinesin motor domainPROSITEPS50067KINESIN_MOTOR_2coord: 19..346
score: 83.509438
IPR036961Kinesin motor domain superfamilyGENE3D3.40.850.10Kinesin motor domaincoord: 9..347
e-value: 1.2E-100
score: 338.8
NoneNo IPR availablePFAMPF12836HHH_3coord: 530..576
e-value: 2.9E-7
score: 30.6
NoneNo IPR availableGENE3D1.10.150.280coord: 516..588
e-value: 3.4E-10
score: 41.4
NoneNo IPR availablePANTHERPTHR47969CHROMOSOME-ASSOCIATED KINESIN KIF4A-RELATEDcoord: 11..594
NoneNo IPR availablePANTHERPTHR47969:SF9BNACNNG40390D PROTEINcoord: 11..594
NoneNo IPR availableCDDcd01376KISc_KID_likecoord: 20..344
e-value: 1.88543E-147
score: 426.919
IPR019821Kinesin motor domain, conserved sitePROSITEPS00411KINESIN_MOTOR_1coord: 248..259
IPR010994RuvA domain 2-likeSUPERFAMILY47781RuvA domain 2-likecoord: 523..587
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 19..374

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02320.1Cp4.1LG03g02320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007018 microtubule-based movement
cellular_component GO:0005871 kinesin complex
cellular_component GO:0005874 microtubule
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0008017 microtubule binding
molecular_function GO:0003777 microtubule motor activity