Cp4.1LG03g02220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g02220
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionADP-ribosylation factor GTPase-activating protein AGD7-like
LocationCp4.1LG03: 645294 .. 647000 (-)
RNA-Seq ExpressionCp4.1LG03g02220
SyntenyCp4.1LG03g02220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCCTTACATTATTCAACAGTTTTTTGTTTACATTTTTCTCAGATTTTTCTTCTGTTTTCGGATTTTCCGATTACCTTTCTTTTTCTTTACTTTTAATCTTCGAATTCATTTGGATCTGATCGAAATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGGTCTGTGTGGATTGCTCCCAGAAGAATCCGCAATGGGCGTCCGTTTCCTATGGCGTTTTCATGTGTTTGGAATGCTCCGGGAAGCACCGTGGCCTTGGTGTTCATATATCTTTTGTTAGATCCGTTACAATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGGGGTAATGAGCACCTCAACGCCTTTCTTGCGCAATACGGAATACCTAAGGAGACGGATATTGTGACTAAATACAATTCTAGTGCGGCTGGAATTTACAGGGATCGAATCCAAGCCCTTGCCGAGGGCCGATCTTGGAAGGATCCACCGGCTGTGAAGGAAAACATTGGAGCAGGAAAATCGAGGCCTCCATTGGCGCAAAGTGCTAGTGGAGGAGCAGGCAGCGGAGGGAGTAAGGTGAACAACGGAGGTTGGGATAATTGGGATAGCGACGACAATTTTAAACCGTCCAATGATATGATGAGGCGTAACCAATCGACTGGCGATGTTCGAGGCATGGGCGGCGGAGGGGTGCATTCCAGGTCCAGATCGACCGAGGATATTTACACACGGTCTCAATTGGAGGCCTCTGCTGCAAATAAAGACAATTTTTTCGCTAAGAAGTTGGCCGAGAATGATTCCCGGCCCGAAGGACTTCCGCCATCACAAGGAGGAAAATATGTTGGATTTGGCTCCTCCAACAATCATGTACCATCTCCAAGAAATGATCCCCAAAGTGACGTTTTCTCTGTTGTTTCTCAGGTATTATGAAGATTCTTCTGTTTGAATTTTCATTTAAGCTTGCTTGCTTGATCAATTGATGCATCGTTTCGTTTCAGGGATTTGGTAAATTATCTCTGGCTGCTGCCTCTGCCGCTCAGTCGGCTGCAAATGCTGTTCAAGCAGGCACAAAGGAGCTGACAACCAAGGTACTTACATTGAAATTGATATCATTATTGAAGAAGAATGAATTTGAATGATTTGTTTATACAGGTGAAGGAGGGAGGGTATGATTACAAAGTGAATGAAACGGTGAATGTTGTGACAGCAAAAACAACAGAGATTGGGCAAAGGACATGGGGAATCATGAGAGGTGTAATGGCAATGGCTTCATTGAAGGTGGAAGAGTACGCCAAAGATGGGATGAACTGGAAGAACGATGGGGGGCAAGGAAACGAGACTGAGAAAAATGAAGAAATTGAGGAGAAGGAGAATGATAAGAAAGGAGGATGGAATTGTTCTTCTGGTGGAACAGGACAAGCATCAAATTCTTGGGATGATTGGGATAATAATAATAATAATAATAATAATAATAATAATAATAATACCAAAGACAACAGCAAAGAGGAAGGAACAACCAAAGGGAGTGGCTCCCATAGTAATGATGGGTGGGCTGGGTGGGATGATCACAAAGATGATGGCTACGACGATCACCATTATGAAGCATCTGTTCCCAAAACTGTGGGCCAAAGTGGTCAAAGTGGTCAAAGTGGGAAGGCCGGTGGCGGTGATACATGGTCGGAGGGAGGGTTTCAACTC

mRNA sequence

TTCCCTTACATTATTCAACAGTTTTTTGTTTACATTTTTCTCAGATTTTTCTTCTGTTTTCGGATTTTCCGATTACCTTTCTTTTTCTTTACTTTTAATCTTCGAATTCATTTGGATCTGATCGAAATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGGTCTGTGTGGATTGCTCCCAGAAGAATCCGCAATGGGCGTCCGTTTCCTATGGCGTTTTCATGTGTTTGGAATGCTCCGGGAAGCACCGTGGCCTTGGTGTTCATATATCTTTTGTTAGATCCGTTACAATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGGGGTAATGAGCACCTCAACGCCTTTCTTGCGCAATACGGAATACCTAAGGAGACGGATATTGTGACTAAATACAATTCTAGTGCGGCTGGAATTTACAGGGATCGAATCCAAGCCCTTGCCGAGGGCCGATCTTGGAAGGATCCACCGGCTGTGAAGGAAAACATTGGAGCAGGAAAATCGAGGCCTCCATTGGCGCAAAGTGCTAGTGGAGGAGCAGGCAGCGGAGGGAGTAAGGTGAACAACGGAGGTTGGGATAATTGGGATAGCGACGACAATTTTAAACCGTCCAATGATATGATGAGGCGTAACCAATCGACTGGCGATGTTCGAGGCATGGGCGGCGGAGGGGTGCATTCCAGGTCCAGATCGACCGAGGATATTTACACACGGTCTCAATTGGAGGCCTCTGCTGCAAATAAAGACAATTTTTTCGCTAAGAAGTTGGCCGAGAATGATTCCCGGCCCGAAGGACTTCCGCCATCACAAGGAGGAAAATATGTTGGATTTGGCTCCTCCAACAATCATGTACCATCTCCAAGAAATGATCCCCAAAGTGACGTTTTCTCTGTTGTTTCTCAGGGATTTGGTAAATTATCTCTGGCTGCTGCCTCTGCCGCTCAGTCGGCTGCAAATGCTGTTCAAGCAGGCACAAAGGAGCTGACAACCAAGGTGAAGGAGGGAGGGTATGATTACAAAGTGAATGAAACGGTGAATGTTGTGACAGCAAAAACAACAGAGATTGGGCAAAGGACATGGGGAATCATGAGAGGTGTAATGGCAATGGCTTCATTGAAGGTGGAAGAGTACGCCAAAGATGGGATGAACTGGAAGAACGATGGGGGGCAAGGAAACGAGACTGAGAAAAATGAAGAAATTGAGGAGAAGGAGAATGATAAGAAAGGAGGATGGAATTGTTCTTCTGGTGGAACAGGACAAGCATCAAATTCTTGGGATGATTGGGATAATAATAATAATAATAATAATAATAATAATAATAATAATACCAAAGACAACAGCAAAGAGGAAGGAACAACCAAAGGGAGTGGCTCCCATAGTAATGATGGGTGGGCTGGGTGGGATGATCACAAAGATGATGGCTACGACGATCACCATTATGAAGCATCTGTTCCCAAAACTGTGGGCCAAAGTGGTCAAAGTGGTCAAAGTGGGAAGGCCGGTGGCGGTGATACATGGTCGGAGGGAGGGTTTCAACTC

Coding sequence (CDS)

ATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGGTCTGTGTGGATTGCTCCCAGAAGAATCCGCAATGGGCGTCCGTTTCCTATGGCGTTTTCATGTGTTTGGAATGCTCCGGGAAGCACCGTGGCCTTGGTGTTCATATATCTTTTGTTAGATCCGTTACAATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGGGGTAATGAGCACCTCAACGCCTTTCTTGCGCAATACGGAATACCTAAGGAGACGGATATTGTGACTAAATACAATTCTAGTGCGGCTGGAATTTACAGGGATCGAATCCAAGCCCTTGCCGAGGGCCGATCTTGGAAGGATCCACCGGCTGTGAAGGAAAACATTGGAGCAGGAAAATCGAGGCCTCCATTGGCGCAAAGTGCTAGTGGAGGAGCAGGCAGCGGAGGGAGTAAGGTGAACAACGGAGGTTGGGATAATTGGGATAGCGACGACAATTTTAAACCGTCCAATGATATGATGAGGCGTAACCAATCGACTGGCGATGTTCGAGGCATGGGCGGCGGAGGGGTGCATTCCAGGTCCAGATCGACCGAGGATATTTACACACGGTCTCAATTGGAGGCCTCTGCTGCAAATAAAGACAATTTTTTCGCTAAGAAGTTGGCCGAGAATGATTCCCGGCCCGAAGGACTTCCGCCATCACAAGGAGGAAAATATGTTGGATTTGGCTCCTCCAACAATCATGTACCATCTCCAAGAAATGATCCCCAAAGTGACGTTTTCTCTGTTGTTTCTCAGGGATTTGGTAAATTATCTCTGGCTGCTGCCTCTGCCGCTCAGTCGGCTGCAAATGCTGTTCAAGCAGGCACAAAGGAGCTGACAACCAAGGTGAAGGAGGGAGGGTATGATTACAAAGTGAATGAAACGGTGAATGTTGTGACAGCAAAAACAACAGAGATTGGGCAAAGGACATGGGGAATCATGAGAGGTGTAATGGCAATGGCTTCATTGAAGGTGGAAGAGTACGCCAAAGATGGGATGAACTGGAAGAACGATGGGGGGCAAGGAAACGAGACTGAGAAAAATGAAGAAATTGAGGAGAAGGAGAATGATAAGAAAGGAGGATGGAATTGTTCTTCTGGTGGAACAGGACAAGCATCAAATTCTTGGGATGATTGGGATAATAATAATAATAATAATAATAATAATAATAATAATAATACCAAAGACAACAGCAAAGAGGAAGGAACAACCAAAGGGAGTGGCTCCCATAGTAATGATGGGTGGGCTGGGTGGGATGATCACAAAGATGATGGCTACGACGATCACCATTATGAAGCATCTGTTCCCAAAACTGTGGGCCAAAGTGGTCAAAGTGGTCAAAGTGGGAAGGCCGGTGGCGGTGATACATGGTCGGAGGGAGGGTTTCAACTC

Protein sequence

MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSWKDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQSTGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGKYVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTTKGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL
Homology
BLAST of Cp4.1LG03g02220 vs. ExPASy Swiss-Prot
Match: Q9M354 (Probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Arabidopsis thaliana OX=3702 GN=AGD6 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.5e-144
Identity = 295/473 (62.37%), Postives = 355/473 (75.05%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+R+LR LQS+P NKVCVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRQLRTLQSQPENKVCVDCAQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWS IQIKKMEAGGNE LN F AQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSAIQIKKMEAGGNERLNKFFAQYGIAKETDIISKYNSNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
            DPP VKE       +PPLAQ   GG G+  +  NNGGWD+WD+DD++K     MRRNQS
Sbjct: 121 NDPPVVKE----ANKKPPLAQ---GGYGNNNNN-NNGGWDSWDNDDSYKSD---MRRNQS 180

Query: 181 TGDVRGMGG-GGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGG 240
             D R  G   G H +S+S+EDIYTRSQLEASAA K++FFA+++AEN+S+PEGLPPSQGG
Sbjct: 181 ANDFRASGNREGAHVKSKSSEDIYTRSQLEASAAGKESFFARRMAENESKPEGLPPSQGG 240

Query: 241 KYVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKV 300
           KYVGFGSS+   P PRN+ Q DVFSVVSQGFG+LSL AASAAQSAA+ VQ GTKE T+KV
Sbjct: 241 KYVGFGSSS--APPPRNNQQDDVFSVVSQGFGRLSLVAASAAQSAASVVQTGTKEFTSKV 300

Query: 301 KEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGN 360
           KEGGYD+KV+ETVNVV  KTTEIG RTWGIM+GVMAMA+ KVEE+ K+G    N   Q +
Sbjct: 301 KEGGYDHKVSETVNVVANKTTEIGHRTWGIMKGVMAMATQKVEEFTKEGSTSWN---QQS 360

Query: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGT 420
           E E N   +   N  K   +   GG  Q+S++   ++N+ N+N+ ++    ++   E   
Sbjct: 361 ENEGNGYYQNFGNGNKAANSSVGGGRPQSSSTSGHYNNSQNSNSWDSWGENENKKTEAVA 420

Query: 421 TKG-SGSHSNDGWAGWDDH--KDDGYDDHHYEASVPKTVGQSGQSGQSGKAGG 470
            KG S S+ +DGW GWDDH  KDDG+D H+  A   K+ G +G+S  +   GG
Sbjct: 421 PKGSSASNDDDGWTGWDDHDAKDDGFDGHYQSAGDKKSAGHNGKSDTAWTGGG 457

BLAST of Cp4.1LG03g02220 vs. ExPASy Swiss-Prot
Match: O80925 (ADP-ribosylation factor GTPase-activating protein AGD7 OS=Arabidopsis thaliana OX=3702 GN=AGD7 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.9e-143
Identity = 298/482 (61.83%), Postives = 356/482 (73.86%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLR LQS+P NKVCVDCSQKNPQWAS+SYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRTLQSQPENKVCVDCSQKNPQWASISYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKM+AGGNE LN FLAQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMDAGGNERLNNFLAQYGISKETDIISKYNSNAASVYRDRIQALAEGRQW 120

Query: 121 KDPPAVKENIGAG--KSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRN 180
           +DPP VKE++G G    +PPL+Q   GG  SG     NGGWDNWD+DD+F+ ++  MRRN
Sbjct: 121 RDPPIVKESVGGGLMNKKPPLSQ--GGGRDSG-----NGGWDNWDNDDSFRSTD--MRRN 180

Query: 181 QSTGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQG 240
           QS GD R  GG G  ++S+S+EDIY+RSQLEASAANK++FFAK++AEN+S+PEGLPPSQG
Sbjct: 181 QSAGDFRSSGGRGAPAKSKSSEDIYSRSQLEASAANKESFFAKRMAENESKPEGLPPSQG 240

Query: 241 GKYVGFGSSNNHVPSPRNDPQS---DVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKEL 300
           GKYVGFGSS    P+PR++ QS   DVFSV+S+GFG+LSL AA    SAAN VQ GT E 
Sbjct: 241 GKYVGFGSSPG--PAPRSNQQSGGGDVFSVMSEGFGRLSLVAA----SAANVVQTGTMEF 300

Query: 301 TTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAK-DGMNW-KN 360
           T+KVKEGG D  V+ETVNVV +KTTEIGQRTWGIM+GVMA+AS KVEE+ K +   W + 
Sbjct: 301 TSKVKEGGLDQTVSETVNVVASKTTEIGQRTWGIMKGVMAIASQKVEEFTKEEASTWNQQ 360

Query: 361 DGGQGNETEKNEEIEEK-ENDKKGGWNCSSGGTGQA---SNSWDDWDNNNNNNNNNNNNN 420
           +  +GN   +N  I  K  N   GG   SS G   +   SNSWDDW   N          
Sbjct: 361 NKTEGNGYYQNSGIGNKTANSSFGGSQSSSSGHNNSYRNSNSWDDWGEEN---------- 420

Query: 421 TKDNSKEEGTTKGSGSHSND--GWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKA 470
              NSK+E   K S S+ +D  GWAGWDD+     D ++  AS  K+VG +G+S  +   
Sbjct: 421 ---NSKKEAAPKVSTSNDDDDGGWAGWDDNDAKDDDFYYQPASDKKSVGHNGKSDTAWTG 454

BLAST of Cp4.1LG03g02220 vs. ExPASy Swiss-Prot
Match: Q62848 (ADP-ribosylation factor GTPase-activating protein 1 OS=Rattus norvegicus OX=10116 GN=Arfgap1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.0e-27
Identity = 128/476 (26.89%), Postives = 207/476 (43.49%), Query Frame = 0

Query: 7   LRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++++  N VC +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEHLNAFL-AQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSWKDPPA 126
           I+++KM+AGGN     FL AQ        +  KY+S AA ++RD++  LAEG+ W    +
Sbjct: 70  IELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESS 129

Query: 127 VKENIGAGKSRPPLAQSASGGAGSGGSKVNNG--GWDNWDSDDNFKPSNDMMRRNQSTGD 186
             +N    + +  L  +A   AG   +   +G   +++W +DD        +   Q   +
Sbjct: 130 PAQNWTPPQPK-TLQFTAHRPAGQPQNVTTSGDKAFEDWLNDD--------LGSYQGAQE 189

Query: 187 VRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGKYVG 246
            R +G G      +  +D                               L  +    Y G
Sbjct: 190 NRYVGFGNTVPPQKREDDF------------------------------LNSAMSSLYSG 249

Query: 247 FGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGT-KELTTKVKEG 306
           + S             S   S   +G  K    A+  A    +++     K    KVKEG
Sbjct: 250 WSSFTTGA--------SKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEG 309

Query: 307 GYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAK---DGMNWKNDGGQGN 366
                V+  V+ + +K   +G + W   R V    S K E+ +    +G +++N  G  +
Sbjct: 310 RIFDDVSSGVSQLASKVQGVGSKGW---RDVTTFFSGKAEDTSDRPLEGHSYQNSSGDNS 369

Query: 367 ----------ETEKNEEIEEKENDKKGGWNCSSGGTG-QASNSWDDWDNNNNNNNNNNNN 426
                     ET  + E  + ++     W C+   TG ++S+SWD W + + +NN N+N 
Sbjct: 370 QNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDIWGSGSASNNKNSN- 414

Query: 427 NTKDNSKEEGTTKGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQS 465
                              +DGW  W+    +G      +A+ P T    G   Q+
Sbjct: 430 -------------------SDGWESWEGASGEGRAKATKKAA-PSTAADEGWDNQN 414

BLAST of Cp4.1LG03g02220 vs. ExPASy Swiss-Prot
Match: Q9EPJ9 (ADP-ribosylation factor GTPase-activating protein 1 OS=Mus musculus OX=10090 GN=Arfgap1 PE=1 SV=2)

HSP 1 Score: 124.0 bits (310), Expect = 4.5e-27
Identity = 124/450 (27.56%), Postives = 202/450 (44.89%), Query Frame = 0

Query: 7   LRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++++  N VC +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEHLNAFL-AQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW--KDP 126
           I+++KM+AGGN     FL  Q        +  KY+S AA ++RD++  LAEG+ W  +  
Sbjct: 70  IELEKMKAGGNAKFREFLETQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESS 129

Query: 127 PAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQSTGD 186
           PA        K+    A  ASG   S  +   +  +++W +DD        +   Q   +
Sbjct: 130 PAQNWTPPQPKTLQFTAHRASGQPQSAAAS-GDKAFEDWLNDD--------LGSYQGAQE 189

Query: 187 VRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGKYVG 246
            R +G G      +  +D                               L  +    Y G
Sbjct: 190 NRYVGFGNTVPPQKREDDF------------------------------LNNAMSSLYSG 249

Query: 247 FGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGT-KELTTKVKEG 306
           + S             S   S   +G  K    A+  A    +++     K    KVKEG
Sbjct: 250 WSSFTTGA--------SKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEG 309

Query: 307 GYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAK---DGMNWKNDGGQGN 366
                V+  V+ + +K   +G + W   R V    S K E+ +    +G +++N  G  +
Sbjct: 310 RIFDDVSSGVSQLASKVQGVGSKGW---RDVTTFFSGKAEDSSDRPLEGHSYQNSSGDNS 369

Query: 367 ----------ETEKNEEIEEKENDKKGGWNCSSGGTG-QASNSWDDWDNNNNNNNNNNNN 426
                     ET  + E  + ++     W C+   TG ++S+SWD W + + +NN N+N+
Sbjct: 370 QNSNIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSSDSWDVWGSGSASNNKNSNS 409

Query: 427 N-------TKDNSKEEGTTKGSGSHSNDGW 432
           +            + + T K + S +++GW
Sbjct: 430 DGWESWEGASGEGRAKATKKAAPSTADEGW 409

BLAST of Cp4.1LG03g02220 vs. ExPASy Swiss-Prot
Match: Q8N6T3 (ADP-ribosylation factor GTPase-activating protein 1 OS=Homo sapiens OX=9606 GN=ARFGAP1 PE=1 SV=2)

HSP 1 Score: 114.8 bits (286), Expect = 2.8e-24
Identity = 130/467 (27.84%), Postives = 209/467 (44.75%), Query Frame = 0

Query: 7   LRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++ +  N VC +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEHLNAFL-AQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW--KDP 126
           I+++KM+AGGN     FL +Q        +  KYNS AA ++RD++ ALAEGR W  +  
Sbjct: 70  IELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESS 129

Query: 127 PAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQSTGD 186
           PA        ++ P +    SG   S  +  ++  +++W +DD               G 
Sbjct: 130 PAQNWTPPQPRTLPSMVHRVSGQPQSVTAS-SDKAFEDWLNDD--------------LGS 189

Query: 187 VRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGKYVG 246
            +G                                                 +QG +YVG
Sbjct: 190 YQG-------------------------------------------------AQGNRYVG 249

Query: 247 FGSSNNHVPSPR-------NDPQSDVFSVVSQ---GFGKLSLAAASAA-----QSAANAV 306
           FG++    P P+       N+  S ++S  S    G  + + AA   A     Q++  A 
Sbjct: 250 FGNT----PPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATKFGSQASQKAS 309

Query: 307 QAG-------TKELTTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKV 366
           + G        K    KVKEG     V+  V+ + +K   +G + W   R V    S K 
Sbjct: 310 ELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQLASKVQGVGSKGW---RDVTTFFSGKA 369

Query: 367 E---EYAKDGMNWKNDG---GQGNETEKN--------EEIEEKENDKKGGWNCSSGGT-G 426
           E   +   +G +++N G    Q +  +++        E  + +++     W C+   T  
Sbjct: 370 EGPLDSPSEGHSYQNSGLDHFQNSNIDQSFWETFGSAEPTKTRKSPSSDSWTCADTSTER 401

Query: 427 QASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTTKG--SGSHSNDGW 432
           ++S+SW+ W + + N N+N++         EGT K       ++DGW
Sbjct: 430 RSSDSWEVWGSASTNRNSNSDG----GEGGEGTKKAVPPAVPTDDGW 401

BLAST of Cp4.1LG03g02220 vs. NCBI nr
Match: XP_023528374.1 (probable ADP-ribosylation factor GTPase-activating protein AGD6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 470/480 (97.92%), Postives = 470/480 (97.92%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTT 420
           TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN          TKDNSKEEGTT
Sbjct: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN----------TKDNSKEEGTT 420

Query: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL 480
           KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL
Sbjct: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL 470

BLAST of Cp4.1LG03g02220 vs. NCBI nr
Match: KAG6580554.1 (ADP-ribosylation factor GTPase-activating protein AGD7, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017309.1 ADP-ribosylation factor GTPase-activating protein AGD7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907 bits (2345), Expect = 0.0
Identity = 467/480 (97.29%), Postives = 467/480 (97.29%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTT 420
           TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN          TKDNSKEEGTT
Sbjct: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN----------TKDNSKEEGTT 420

Query: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL 480
           KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG   KAGGGDTWSEGGFQL
Sbjct: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG---KAGGGDTWSEGGFQL 467

BLAST of Cp4.1LG03g02220 vs. NCBI nr
Match: XP_022934331.1 (ADP-ribosylation factor GTPase-activating protein AGD7-like [Cucurbita moschata])

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 466/480 (97.08%), Postives = 467/480 (97.29%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTT 420
           TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDN+N          TKDNSKEEGTT
Sbjct: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNSN----------TKDNSKEEGTT 420

Query: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL 480
           KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG   KAGGGDTWSEGGFQL
Sbjct: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG---KAGGGDTWSEGGFQL 467

BLAST of Cp4.1LG03g02220 vs. NCBI nr
Match: XP_022983985.1 (probable ADP-ribosylation factor GTPase-activating protein AGD6 [Cucurbita maxima] >XP_022983986.1 probable ADP-ribosylation factor GTPase-activating protein AGD6 [Cucurbita maxima])

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 458/481 (95.22%), Postives = 458/481 (95.22%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIG GKSRPPLAQSASGG G GGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSASGGGGGGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNN VPSPRNDP SDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNSVPSPRNDPHSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGG-QGN 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGG QGN
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGGQGN 360

Query: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGT 420
           ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN          TK N KEEGT
Sbjct: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN----------TKHNGKEEGT 420

Query: 421 TKGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQ 480
           TKGSGSHSNDGWAGWDDHKDDGYDDHHYEAS PKTVGQSGQSG   K GGGDTWSEGGFQ
Sbjct: 421 TKGSGSHSNDGWAGWDDHKDDGYDDHHYEASDPKTVGQSGQSG---KPGGGDTWSEGGFQ 468

BLAST of Cp4.1LG03g02220 vs. NCBI nr
Match: XP_038904762.1 (ADP-ribosylation factor GTPase-activating protein AGD7-like [Benincasa hispida])

HSP 1 Score: 728 bits (1879), Expect = 3.98e-261
Identity = 400/494 (80.97%), Postives = 423/494 (85.63%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNK+CVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNE LNAFLAQYGIPKETDIVTKYN+SAAG+YRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLAQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGG--SKVNNGGWDNWDSDDNFKPSNDMMRRN 180
           KDPP VKENIG GKSRPPLAQSA GG G GG  SKVNNGGWD+WD+DDNF+ S+DM RRN
Sbjct: 121 KDPPPVKENIGVGKSRPPLAQSAGGGGGGGGGGSKVNNGGWDSWDNDDNFRSSSDM-RRN 180

Query: 181 QSTGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQG 240
           QSTGDVRGMGGGG+ SRS+STEDIYTRSQLEASAANKDNFFA+K+AENDSRPEG+PPSQG
Sbjct: 181 QSTGDVRGMGGGGMPSRSKSTEDIYTRSQLEASAANKDNFFARKIAENDSRPEGIPPSQG 240

Query: 241 GKYVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTK 300
           GKYVGFGSS    P+ RNDPQ+DVFSVVSQGFGKLSL AASAAQSAANAVQAGTKELTTK
Sbjct: 241 GKYVGFGSSP--APAQRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTK 300

Query: 301 VKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQG 360
           VKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMAS KVEEY KDGMNWKNDG Q 
Sbjct: 301 VKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYTKDGMNWKNDGWQR 360

Query: 361 NETEKNEEIEEKENDKKGGWNCSSGGTGQAS----------NSWDDWDNNNNNNNNNNNN 420
           NE EKN   +E E+D KG WN SSG TGQ+S          +SWDDWD            
Sbjct: 361 NENEKNGYYQEFEHDNKG-WNSSSG-TGQSSGSGHNNSYNSSSWDDWD------------ 420

Query: 421 NTKDNSKEEGTTKGSGSH----SNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQS 478
            TKDN KEE TTKGSG+H    SNDGWAGWDD KDDGYD++ Y+AS  KT GQ+G     
Sbjct: 421 -TKDNRKEETTTKGSGNHNNNNSNDGWAGWDDQKDDGYDNY-YQASDRKTAGQNG----- 468

BLAST of Cp4.1LG03g02220 vs. ExPASy TrEMBL
Match: A0A6J1F1I3 (ADP-ribosylation factor GTPase-activating protein AGD7-like OS=Cucurbita moschata OX=3662 GN=LOC111441528 PE=4 SV=1)

HSP 1 Score: 905 bits (2340), Expect = 0.0
Identity = 466/480 (97.08%), Postives = 467/480 (97.29%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGTT 420
           TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDN+N          TKDNSKEEGTT
Sbjct: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNSN----------TKDNSKEEGTT 420

Query: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQL 480
           KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG   KAGGGDTWSEGGFQL
Sbjct: 421 KGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSG---KAGGGDTWSEGGFQL 467

BLAST of Cp4.1LG03g02220 vs. ExPASy TrEMBL
Match: A0A6J1J7F5 (probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Cucurbita maxima OX=3661 GN=LOC111482439 PE=4 SV=1)

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 458/481 (95.22%), Postives = 458/481 (95.22%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIG GKSRPPLAQSASGG G GGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSASGGGGGGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK
Sbjct: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSSNN VPSPRNDP SDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSNNSVPSPRNDPHSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGG-QGN 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGG QGN
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGGQGN 360

Query: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGT 420
           ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN          TK N KEEGT
Sbjct: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNN----------TKHNGKEEGT 420

Query: 421 TKGSGSHSNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKAGGGDTWSEGGFQ 480
           TKGSGSHSNDGWAGWDDHKDDGYDDHHYEAS PKTVGQSGQSG   K GGGDTWSEGGFQ
Sbjct: 421 TKGSGSHSNDGWAGWDDHKDDGYDDHHYEASDPKTVGQSGQSG---KPGGGDTWSEGGFQ 468

BLAST of Cp4.1LG03g02220 vs. ExPASy TrEMBL
Match: A0A1S3B6M7 (ADP-ribosylation factor GTPase-activating protein AGD7 OS=Cucumis melo OX=3656 GN=LOC103486399 PE=4 SV=1)

HSP 1 Score: 725 bits (1871), Expect = 2.84e-260
Identity = 400/495 (80.81%), Postives = 422/495 (85.25%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNK+CVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNE LNAFLAQYGIPKETDIVTKYN+SAAG+YRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLAQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIG GKSRPPLAQSA GG    GSKVNNGGWD+WD+DDNF+ S+DM RRNQS
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGG----GSKVNNGGWDSWDNDDNFRSSSDM-RRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRG GGGG+ SRSRSTEDIYTR+QLEASAANKDNFFA+K+AENDSRPEG+PPSQGGK
Sbjct: 181 TGDVRGTGGGGMPSRSRSTEDIYTRAQLEASAANKDNFFARKIAENDSRPEGIPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSS    P+ RNDPQ+DVFSVVSQGFGKLSL AASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSP--TPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMAS KVEEYAKDGMNWKNDG Q NE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQAS-----------NSWDDWDNNNNNNNNNNNNN 420
            EKN   +E E+D KG WN SSG TGQ+S           +SWDDWD             
Sbjct: 361 NEKNGYYQEFEHDNKG-WNSSSG-TGQSSGSGHHNNSYNSSSWDDWD------------- 420

Query: 421 TKDNSKEEGTTKGSGSH------SNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQ 478
           TKDN KEE TTK SG+H      SNDGWAGWDD KDDGYD H+Y+AS  KTVGQ+G    
Sbjct: 421 TKDNRKEETTTKVSGTHNNNNNNSNDGWAGWDDQKDDGYD-HYYQASDRKTVGQNG---- 465

BLAST of Cp4.1LG03g02220 vs. ExPASy TrEMBL
Match: A0A5D3DN63 (ADP-ribosylation factor GTPase-activating protein AGD7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001780 PE=4 SV=1)

HSP 1 Score: 725 bits (1871), Expect = 2.84e-260
Identity = 400/495 (80.81%), Postives = 422/495 (85.25%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNK+CVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNE LNAFLAQYGIPKETDIVTKYN+SAAG+YRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLAQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIG GKSRPPLAQSA GG    GSKVNNGGWD+WD+DDNF+ S+DM RRNQS
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGG----GSKVNNGGWDSWDNDDNFRSSSDM-RRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRG GGGG+ SRSRSTEDIYTR+QLEASAANKDNFFA+K+AENDSRPEG+PPSQGGK
Sbjct: 181 TGDVRGTGGGGMPSRSRSTEDIYTRAQLEASAANKDNFFARKIAENDSRPEGIPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSS    P+ RNDPQ+DVFSVVSQGFGKLSL AASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSP--TPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMAS KVEEYAKDGMNWKNDG Q NE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQAS-----------NSWDDWDNNNNNNNNNNNNN 420
            EKN   +E E+D KG WN SSG TGQ+S           +SWDDWD             
Sbjct: 361 NEKNGYYQEFEHDNKG-WNSSSG-TGQSSGSGHHNNSYNSSSWDDWD------------- 420

Query: 421 TKDNSKEEGTTKGSGSH------SNDGWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQ 478
           TKDN KEE TTK SG+H      SNDGWAGWDD KDDGYD H+Y+AS  KTVGQ+G    
Sbjct: 421 TKDNRKEETTTKVSGTHNNNNNNSNDGWAGWDDQKDDGYD-HYYQASDRKTVGQNG---- 465

BLAST of Cp4.1LG03g02220 vs. ExPASy TrEMBL
Match: A0A0A0LAL4 (Arf-GAP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734360 PE=4 SV=1)

HSP 1 Score: 714 bits (1842), Expect = 7.94e-256
Identity = 399/500 (79.80%), Postives = 420/500 (84.00%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNK+CVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNE LNAFL+QYGIPKETDIVTKYN+SAAG+YRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
           KDPPAVKENIG GKSRPPLAQSA GG    GSKVNNGGWDN   +DNF+ S+DM RRNQS
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGG----GSKVNNGGWDN---EDNFRSSSDM-RRNQS 180

Query: 181 TGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGGK 240
           TGDVRGMGGGG+ SRSRSTEDIYTRSQLEASAANKDNFFA+K+AENDSRPEG+PPSQGGK
Sbjct: 181 TGDVRGMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGK 240

Query: 241 YVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKVK 300
           YVGFGSS    P+ RNDPQ+DVFSVVSQGFGKLSL AASAAQSAANAVQAGTKELTTKVK
Sbjct: 241 YVGFGSSP--APARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVK 300

Query: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGNE 360
           EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMAS KVEEYAKDGMNWKNDG Q NE
Sbjct: 301 EGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNE 360

Query: 361 TEKNEEIEEKENDKKGGWNCSSGGTGQAS-----------NSWDDWDNNNNNNNNNNNNN 420
            EKN   +E E+D KG WN SSG TGQ+S           +SWDDWD             
Sbjct: 361 NEKNGYYQEFEHDNKG-WNSSSG-TGQSSGSGHHNNSYNSSSWDDWD------------- 420

Query: 421 TKDNSKEEGTTKGSGSH-----------SNDGWAGWDDHKDDGYDDHHYEASVPKTVGQS 478
           TKDN KEE TTK SG+H           SNDGWAGWDD KDDGYD H+Y+AS  KTVGQ+
Sbjct: 421 TKDNRKEETTTKVSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYD-HYYQASDRKTVGQN 467

BLAST of Cp4.1LG03g02220 vs. TAIR 10
Match: AT3G53710.1 (ARF-GAP domain 6 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-145
Identity = 295/473 (62.37%), Postives = 355/473 (75.05%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+R+LR LQS+P NKVCVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRQLRTLQSQPENKVCVDCAQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWS IQIKKMEAGGNE LN F AQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSAIQIKKMEAGGNERLNKFFAQYGIAKETDIISKYNSNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
            DPP VKE       +PPLAQ   GG G+  +  NNGGWD+WD+DD++K     MRRNQS
Sbjct: 121 NDPPVVKE----ANKKPPLAQ---GGYGNNNNN-NNGGWDSWDNDDSYKSD---MRRNQS 180

Query: 181 TGDVRGMGG-GGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGG 240
             D R  G   G H +S+S+EDIYTRSQLEASAA K++FFA+++AEN+S+PEGLPPSQGG
Sbjct: 181 ANDFRASGNREGAHVKSKSSEDIYTRSQLEASAAGKESFFARRMAENESKPEGLPPSQGG 240

Query: 241 KYVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKV 300
           KYVGFGSS+   P PRN+ Q DVFSVVSQGFG+LSL AASAAQSAA+ VQ GTKE T+KV
Sbjct: 241 KYVGFGSSS--APPPRNNQQDDVFSVVSQGFGRLSLVAASAAQSAASVVQTGTKEFTSKV 300

Query: 301 KEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGN 360
           KEGGYD+KV+ETVNVV  KTTEIG RTWGIM+GVMAMA+ KVEE+ K+G    N   Q +
Sbjct: 301 KEGGYDHKVSETVNVVANKTTEIGHRTWGIMKGVMAMATQKVEEFTKEGSTSWN---QQS 360

Query: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGT 420
           E E N   +   N  K   +   GG  Q+S++   ++N+ N+N+ ++    ++   E   
Sbjct: 361 ENEGNGYYQNFGNGNKAANSSVGGGRPQSSSTSGHYNNSQNSNSWDSWGENENKKTEAVA 420

Query: 421 TKG-SGSHSNDGWAGWDDH--KDDGYDDHHYEASVPKTVGQSGQSGQSGKAGG 470
            KG S S+ +DGW GWDDH  KDDG+D H+  A   K+ G +G+S  +   GG
Sbjct: 421 PKGSSASNDDDGWTGWDDHDAKDDGFDGHYQSAGDKKSAGHNGKSDTAWTGGG 457

BLAST of Cp4.1LG03g02220 vs. TAIR 10
Match: AT3G53710.2 (ARF-GAP domain 6 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.5e-145
Identity = 295/473 (62.37%), Postives = 355/473 (75.05%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+R+LR LQS+P NKVCVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRQLRTLQSQPENKVCVDCAQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWS IQIKKMEAGGNE LN F AQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSAIQIKKMEAGGNERLNKFFAQYGIAKETDIISKYNSNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGAGKSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRNQS 180
            DPP VKE       +PPLAQ   GG G+  +  NNGGWD+WD+DD++K     MRRNQS
Sbjct: 121 NDPPVVKE----ANKKPPLAQ---GGYGNNNNN-NNGGWDSWDNDDSYKSD---MRRNQS 180

Query: 181 TGDVRGMGG-GGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQGG 240
             D R  G   G H +S+S+EDIYTRSQLEASAA K++FFA+++AEN+S+PEGLPPSQGG
Sbjct: 181 ANDFRASGNREGAHVKSKSSEDIYTRSQLEASAAGKESFFARRMAENESKPEGLPPSQGG 240

Query: 241 KYVGFGSSNNHVPSPRNDPQSDVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKELTTKV 300
           KYVGFGSS+   P PRN+ Q DVFSVVSQGFG+LSL AASAAQSAA+ VQ GTKE T+KV
Sbjct: 241 KYVGFGSSS--APPPRNNQQDDVFSVVSQGFGRLSLVAASAAQSAASVVQTGTKEFTSKV 300

Query: 301 KEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAKDGMNWKNDGGQGN 360
           KEGGYD+KV+ETVNVV  KTTEIG RTWGIM+GVMAMA+ KVEE+ K+G    N   Q +
Sbjct: 301 KEGGYDHKVSETVNVVANKTTEIGHRTWGIMKGVMAMATQKVEEFTKEGSTSWN---QQS 360

Query: 361 ETEKNEEIEEKENDKKGGWNCSSGGTGQASNSWDDWDNNNNNNNNNNNNNTKDNSKEEGT 420
           E E N   +   N  K   +   GG  Q+S++   ++N+ N+N+ ++    ++   E   
Sbjct: 361 ENEGNGYYQNFGNGNKAANSSVGGGRPQSSSTSGHYNNSQNSNSWDSWGENENKKTEAVA 420

Query: 421 TKG-SGSHSNDGWAGWDDH--KDDGYDDHHYEASVPKTVGQSGQSGQSGKAGG 470
            KG S S+ +DGW GWDDH  KDDG+D H+  A   K+ G +G+S  +   GG
Sbjct: 421 PKGSSASNDDDGWTGWDDHDAKDDGFDGHYQSAGDKKSAGHNGKSDTAWTGGG 457

BLAST of Cp4.1LG03g02220 vs. TAIR 10
Match: AT2G37550.1 (ARF-GAP domain 7 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-144
Identity = 298/482 (61.83%), Postives = 356/482 (73.86%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLR LQS+P NKVCVDCSQKNPQWAS+SYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRTLQSQPENKVCVDCSQKNPQWASISYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKM+AGGNE LN FLAQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMDAGGNERLNNFLAQYGISKETDIISKYNSNAASVYRDRIQALAEGRQW 120

Query: 121 KDPPAVKENIGAG--KSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRN 180
           +DPP VKE++G G    +PPL+Q   GG  SG     NGGWDNWD+DD+F+ ++  MRRN
Sbjct: 121 RDPPIVKESVGGGLMNKKPPLSQ--GGGRDSG-----NGGWDNWDNDDSFRSTD--MRRN 180

Query: 181 QSTGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQG 240
           QS GD R  GG G  ++S+S+EDIY+RSQLEASAANK++FFAK++AEN+S+PEGLPPSQG
Sbjct: 181 QSAGDFRSSGGRGAPAKSKSSEDIYSRSQLEASAANKESFFAKRMAENESKPEGLPPSQG 240

Query: 241 GKYVGFGSSNNHVPSPRNDPQS---DVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKEL 300
           GKYVGFGSS    P+PR++ QS   DVFSV+S+GFG+LSL AA    SAAN VQ GT E 
Sbjct: 241 GKYVGFGSSPG--PAPRSNQQSGGGDVFSVMSEGFGRLSLVAA----SAANVVQTGTMEF 300

Query: 301 TTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAK-DGMNW-KN 360
           T+KVKEGG D  V+ETVNVV +KTTEIGQRTWGIM+GVMA+AS KVEE+ K +   W + 
Sbjct: 301 TSKVKEGGLDQTVSETVNVVASKTTEIGQRTWGIMKGVMAIASQKVEEFTKEEASTWNQQ 360

Query: 361 DGGQGNETEKNEEIEEK-ENDKKGGWNCSSGGTGQA---SNSWDDWDNNNNNNNNNNNNN 420
           +  +GN   +N  I  K  N   GG   SS G   +   SNSWDDW   N          
Sbjct: 361 NKTEGNGYYQNSGIGNKTANSSFGGSQSSSSGHNNSYRNSNSWDDWGEEN---------- 420

Query: 421 TKDNSKEEGTTKGSGSHSND--GWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKA 470
              NSK+E   K S S+ +D  GWAGWDD+     D ++  AS  K+VG +G+S  +   
Sbjct: 421 ---NSKKEAAPKVSTSNDDDDGGWAGWDDNDAKDDDFYYQPASDKKSVGHNGKSDTAWTG 454

BLAST of Cp4.1LG03g02220 vs. TAIR 10
Match: AT2G37550.2 (ARF-GAP domain 7 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.1e-144
Identity = 298/482 (61.83%), Postives = 356/482 (73.86%), Query Frame = 0

Query: 1   MAASRRLRDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLR LQS+P NKVCVDCSQKNPQWAS+SYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRTLQSQPENKVCVDCSQKNPQWASISYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRIQALAEGRSW 120
           MDSWSEIQIKKM+AGGNE LN FLAQYGI KETDI++KYNS+AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMDAGGNERLNNFLAQYGISKETDIISKYNSNAASVYRDRIQALAEGRQW 120

Query: 121 KDPPAVKENIGAG--KSRPPLAQSASGGAGSGGSKVNNGGWDNWDSDDNFKPSNDMMRRN 180
           +DPP VKE++G G    +PPL+Q   GG  SG     NGGWDNWD+DD+F+ ++  MRRN
Sbjct: 121 RDPPIVKESVGGGLMNKKPPLSQ--GGGRDSG-----NGGWDNWDNDDSFRSTD--MRRN 180

Query: 181 QSTGDVRGMGGGGVHSRSRSTEDIYTRSQLEASAANKDNFFAKKLAENDSRPEGLPPSQG 240
           QS GD R  GG G  ++S+S+EDIY+RSQLEASAANK++FFAK++AEN+S+PEGLPPSQG
Sbjct: 181 QSAGDFRSSGGRGAPAKSKSSEDIYSRSQLEASAANKESFFAKRMAENESKPEGLPPSQG 240

Query: 241 GKYVGFGSSNNHVPSPRNDPQS---DVFSVVSQGFGKLSLAAASAAQSAANAVQAGTKEL 300
           GKYVGFGSS    P+PR++ QS   DVFSV+S+GFG+LSL AA    SAAN VQ GT E 
Sbjct: 241 GKYVGFGSSPG--PAPRSNQQSGGGDVFSVMSEGFGRLSLVAA----SAANVVQTGTMEF 300

Query: 301 TTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASLKVEEYAK-DGMNW-KN 360
           T+KVKEGG D  V+ETVNVV +KTTEIGQRTWGIM+GVMA+AS KVEE+ K +   W + 
Sbjct: 301 TSKVKEGGLDQTVSETVNVVASKTTEIGQRTWGIMKGVMAIASQKVEEFTKEEASTWNQQ 360

Query: 361 DGGQGNETEKNEEIEEK-ENDKKGGWNCSSGGTGQA---SNSWDDWDNNNNNNNNNNNNN 420
           +  +GN   +N  I  K  N   GG   SS G   +   SNSWDDW   N          
Sbjct: 361 NKTEGNGYYQNSGIGNKTANSSFGGSQSSSSGHNNSYRNSNSWDDWGEEN---------- 420

Query: 421 TKDNSKEEGTTKGSGSHSND--GWAGWDDHKDDGYDDHHYEASVPKTVGQSGQSGQSGKA 470
              NSK+E   K S S+ +D  GWAGWDD+     D ++  AS  K+VG +G+S  +   
Sbjct: 421 ---NSKKEAAPKVSTSNDDDDGGWAGWDDNDAKDDDFYYQPASDKKSVGHNGKSDTAWTG 454

BLAST of Cp4.1LG03g02220 vs. TAIR 10
Match: AT5G46750.1 (ARF-GAP domain 9 )

HSP 1 Score: 120.9 bits (302), Expect = 2.7e-27
Identity = 55/104 (52.88%), Postives = 67/104 (64.42%), Query Frame = 0

Query: 8   RDLQSRPGNKVCVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEI 67
           R L+S+  NKVC DCS KNP WASV YG+F+C++CS  HR LGVHISFVRS  +DSWS  
Sbjct: 14  RKLKSKSENKVCFDCSAKNPTWASVPYGIFLCIDCSAVHRSLGVHISFVRSTNLDSWSPE 73

Query: 68  QIKKMEAGGNEHLNAFLAQYGIPKETDIVTKYNSSAAGIYRDRI 112
           Q++ M  GGN     F  Q+G      I  KY S AA +YR  +
Sbjct: 74  QLRTMMFGGNNRAQVFFKQHGWNDGGKIEAKYTSRAADMYRQTL 117

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M3543.5e-14462.37Probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Arabidopsis t... [more]
O809252.9e-14361.83ADP-ribosylation factor GTPase-activating protein AGD7 OS=Arabidopsis thaliana O... [more]
Q628482.0e-2726.89ADP-ribosylation factor GTPase-activating protein 1 OS=Rattus norvegicus OX=1011... [more]
Q9EPJ94.5e-2727.56ADP-ribosylation factor GTPase-activating protein 1 OS=Mus musculus OX=10090 GN=... [more]
Q8N6T32.8e-2427.84ADP-ribosylation factor GTPase-activating protein 1 OS=Homo sapiens OX=9606 GN=A... [more]
Match NameE-valueIdentityDescription
XP_023528374.10.097.92probable ADP-ribosylation factor GTPase-activating protein AGD6 [Cucurbita pepo ... [more]
KAG6580554.10.097.29ADP-ribosylation factor GTPase-activating protein AGD7, partial [Cucurbita argyr... [more]
XP_022934331.10.097.08ADP-ribosylation factor GTPase-activating protein AGD7-like [Cucurbita moschata][more]
XP_022983985.10.095.22probable ADP-ribosylation factor GTPase-activating protein AGD6 [Cucurbita maxim... [more]
XP_038904762.13.98e-26180.97ADP-ribosylation factor GTPase-activating protein AGD7-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F1I30.097.08ADP-ribosylation factor GTPase-activating protein AGD7-like OS=Cucurbita moschat... [more]
A0A6J1J7F50.095.22probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Cucurbita max... [more]
A0A1S3B6M72.84e-26080.81ADP-ribosylation factor GTPase-activating protein AGD7 OS=Cucumis melo OX=3656 G... [more]
A0A5D3DN632.84e-26080.81ADP-ribosylation factor GTPase-activating protein AGD7 OS=Cucumis melo var. maku... [more]
A0A0A0LAL47.94e-25679.80Arf-GAP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G734360 PE=... [more]
Match NameE-valueIdentityDescription
AT3G53710.12.5e-14562.37ARF-GAP domain 6 [more]
AT3G53710.22.5e-14562.37ARF-GAP domain 6 [more]
AT2G37550.12.1e-14461.83ARF-GAP domain 7 [more]
AT2G37550.22.1e-14461.83ARF-GAP domain 7 [more]
AT5G46750.12.7e-2752.88ARF-GAP domain 9 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001164Arf GTPase activating proteinPRINTSPR00405REVINTRACTNGcoord: 56..77
score: 44.2
coord: 35..52
score: 60.15
coord: 16..35
score: 50.0
IPR001164Arf GTPase activating proteinSMARTSM00105arf_gap_3coord: 1..120
e-value: 1.6E-44
score: 163.9
IPR001164Arf GTPase activating proteinPFAMPF01412ArfGapcoord: 5..94
e-value: 7.4E-34
score: 116.3
IPR001164Arf GTPase activating proteinPROSITEPS50115ARFGAPcoord: 4..120
score: 26.119831
IPR038508ArfGAP domain superfamilyGENE3D1.10.220.150Arf GTPase activating proteincoord: 1..131
e-value: 9.7E-47
score: 160.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 145..162
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..260
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 453..468
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 349..480
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 378..413
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 122..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..450
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 358..376
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..260
NoneNo IPR availablePANTHERPTHR47021:SF4ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD6-RELATEDcoord: 1..467
NoneNo IPR availableCDDcd08830ArfGap_ArfGap1coord: 3..117
e-value: 2.83222E-76
score: 232.773
IPR044519ADP-ribosylation factor GTPase-activating protein AGD6/7PANTHERPTHR47021ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD6-RELATEDcoord: 1..467
IPR037278ARFGAP/RecO-like zinc fingerSUPERFAMILY57863ArfGap/RecO-like zinc fingercoord: 5..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g02220.1Cp4.1LG03g02220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0016192 vesicle-mediated transport
molecular_function GO:0005096 GTPase activator activity