Cp4.1LG03g01660 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g01660
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	4-coumarate-CoA ligase
Location	Cp4.1LG03: 982403 .. 986048 (-)
RNA-Seq Expression	Cp4.1LG03g01660
Synteny	Cp4.1LG03g01660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCGCGATCGCGATGGCCAAATCTTTCAATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTTCGAAACTCCTCCTCTTTCCTTCACACCCTCGCTCTCACCGACGCGGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCCAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGGAAAGGCGATGTGGTTCTCATATTCGCGCCTAATTCCATCCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGCGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTGTAATCCTGGGTTCTAAAATTTCTTCGGAATCCTCCCGCCCCAATATCTGGGTTTACTCCGATTTGATCAAAAACTCCGGCGACGTGTCCGATTTGCCTGTGAGTGAAGTGGGTCAGAGCGATGTGGCGGCACTATTGTATTCATCTGGGACGACGGGGATTAGCAAGGGAGTGATTCTGACTCATAGAAACTTCATAACGACGTCGTTAATGGTGACCCATGATCAGGACCTTCTTGGTGATCCTAGAAACGTGTTCTTATGCTTTCTCCCAATGTTCCATGTATTTGGTCTCTCTGTAATTCTTTACTCGCAGCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGGGTGGTGATGAAGTATAGAATCACTCATCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAGGTATGACTTGTCGTCGTTGAAGCAGATTTTATCGGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGTTTTGAACTGTTCCTTCTCAACTCCTCCATTTTGCACTTTCATTTGGCTGTTACAGGAAATTTTGTATGGTGTACAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCAGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGTGGATCTCTCTTTGGATATTCTTTAAATGATTTTGAATCGCAGGTGAACATAATTTTGATCTGATGAACAGTGCTAATCCATACTAACTATACTGAAGGGTGTATCAAACACCAAACTAGAGTTATACCTTGATCCAACCCTTCTCTTTTGTAACGGTTCAAGCCAATCGCTAACAAATATTGTCCGTTTTCAACGTGATACGTATCGCCACCAGTCCCACGGTTTTAAAACGCGTATGTTAGGGGGAGGTTTCTATACCTTTATGAAGAATGTTTCATTATCCCCCAACTGATGTGGGATCTCATAATCCACCCCTTCCGGGCCTAGCGTCCTTGTTGGCACATCGCCTCGTGTCCACCCCCTTCAAAGCTCAATCTCCTTGCTGGAGCTCGGTGTCTGACTCTGATACCATTTGTAATAGCCCAAGCCCACAGCTAGAAATATTGTCTACTTTGGTCTCTTTTCTAAGAGTAAAATTGTGAGATCCTACATCATGGGGAGGAGAACGAAACATACTTCATAAGGGTGTGAAAACCTCTCCCTAGCAGACGCGTTTTAAAACCGTGAGGCTGACGGCAATACGTAACGGGCCACTTTCTTGAACTTGTTTGATTGGATCTGCTAAAAAGAATTGAAATTTCTCCCTGTTTTAAAGATGCCAATCTCGTTTCTCTGATGATTTGTTGTCAGTCTGATTGGCTAAATGTTTGCATGAACATTTCAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTATCAGGTTGTTTTTTACCTCAACAAACATCAACTTTTCCATTTGGGAGTGCTTCATTTCCTTTTACTAGGGCGGTCCAAACTCATCACTAATAGATATGTCTTCTTTAGGCTTTTCCTTTAAAACGCGTTTGTTAGGGAGAGGTTTCCATACCCTTATTTATAAAGAATGCTTCATTCTCCTCTCCAACGGACGTGAGATCTCACAAACCATTATATCAGAACTTAACTTCTCTTAAATCAAAAGAAACTGCTATAGATTCTTGTATGCTAAGTTCTTCTCAGTTAAAGAATATGATTATAAGATGTACTAATCATATGCTCGGCTCCAGCAAGCATGTCTTCTAATGCTATGTCTGCATTGTCTCCTCTTCTTGGGAGTATCAACTCTAGCTGCATTCGTTGTTGATTGATATCAATTTTTTTGTAACTTTCTTGTCTGCATATTAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGATGCCATTGTGATCCCGTAAGGAGTATATAGTAGCATCTATAGTCCCATCATTATGTGGACGTTCATAGTGGATTTTCCTTTCTATTTCATACTTTCTTGTTAACCTCTGCCATGAATTTCCACCTGCAGATATCCTGATGATAAAGCCGGTGAGGTCCCTATAGCATTTGTTGTTCGTTCACCTAATAACTCAATAAACGAAGAAGATGTTAAAAGTTTTATCGCGGGTCAGGTAATATTTTGCAAACCCCTCAAACAAGAACAATAAAAAGACTTTTTGTCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCTCTAATAAACGCGTTTTAAAAACCTTGAGGGAAAGCCTAAAAGGAAAAGCTTAAGAGGACAATATCTGCGAGCGATGGCATGCCCCCACCTCACGAATTGGGGTTGCTGTGTCTTTTCCTAAGAGGGGCAGCGGCAAGCCAAGCCTTTACTAAGAGGCCGCCTTTTATGGGCCTGCTGCATAAACCCAAGAAGGCAAAAGATGTGGAAAAATGCAAATTTAAACATTGATTCCCTACGCAAAAAGTGGGAACCATCATTTTGACAAAGGAATTCATGAAAATGATCAGCTCTTTCCCTTTGCAGGTTGCACCATTCAAGAGATTAAAAATAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAGACGTCAAAATATTGAATAGAAAGAACGCAAGTTATTCTGATACAGTTAGCAAAGAAATGTATTTACAGCTTGATGAACACATGAACAATGCAACAACAATTTACATGGATCATAATAAATAACAGCTTTACTTTTGGCAAAAAGAGAATCATTTCAGAGCCAATAAAAGCAAAAGGAAACAGAAAAGTGTATGATTTAAAATGGAGTGTGAAGATGAGTAAGAGATGAAGTAGATGAT

mRNA sequence

ATGGCGATCGCGATCGCGATGGCCAAATCTTTCAATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTTCGAAACTCCTCCTCTTTCCTTCACACCCTCGCTCTCACCGACGCGGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCCAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGGAAAGGCGATGTGGTTCTCATATTCGCGCCTAATTCCATCCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGCGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGGGTGGTGATGAAGTATAGAATCACTCATCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAGGTATGACTTGTCGTCGTTGAAGCAGATTTTATCGGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCAGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTATCAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGATGCCATTGTGATCCCATATCCTGATGATAAAGCCGGTGAGGTCCCTATAGCATTTGTTGTTGCACCATTCAAGAGATTAAAAATAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAGACGTCAAAATATTGAATAGAAAGAACGCAAGTTATTCTGATACAGTTAGCAAAGAAATGTATTTACAGCTTGATGAACACATGAACAATGCAACAACAATTTACATGGATCATAATAAATAACAGCTTTACTTTTGGCAAAAAGAGAATCATTTCAGAGCCAATAAAAGCAAAAGGAAACAGAAAAGTGTATGATTTAAAATGGAGTGTGAAGATGAGTAAGAGATGAAGTAGATGAT

Coding sequence (CDS)

ATGGCGATCGCGATCGCGATGGCCAAATCTTTCAATCCCCAATCCCAGATTTACACTTCCCCACGTCCCCCAATCCACTTTCCGACCGACCCCACTATCTCCATTGTTTCGTTTCTCTTTCGAAACTCCTCCTCTTTCCTTCACACCCTCGCTCTCACCGACGCGGATTCCGGCGAATCCCTAACTTTCCGTCAGCTTCAAATCCAAGTCTCCCAGTTGGCCCATGCGTTTATCCACCTCGGGATTCGGAAAGGCGATGTGGTTCTCATATTCGCGCCTAATTCCATCCATTTCCCTGTTTGTTTCTTCGCCATTGTTGCCATTGGAGCCATCGCCACCACTTGTAACCCTGCTTACACCTTCGCGGAACTATCAAAGCAAGTCGCGAACTGCAACCCCAAGCTCGTAATCACCGTTCCAGAACTCTGGGACGTAATCGGTAAACTCAACTTGCCTTCTCTTCAAAGAGGAAACACCGTCGTTTCAATGGCGAAGTTTGAGCTGGAAAAGGCTTTGGGGGTGGTGATGAAGTATAGAATCACTCATCTTTATCTTGTTCCGCCTGTAATTATAGCCATGGCGAAGCAAAGTGTGGTGAAGAGGTATGACTTGTCGTCGTTGAAGCAGATTTTATCGGGGGCGGCGCCTCTTGGGAAGGATGTGATGGAAGAATGTTCTAAGGTTTTACCTCAGACCAAGATCACGCAGGGGTATGGTATGACAGAAACTTGTGGAGTTATTTCAATGGAGAATTTGGGGGTCGAATCTAGACTATCAGGGTCAACTGGATTCCTTGTTTCAGGAATTGAGGCCCAAATTTTGAGTACAGAAACGCAGAAGCGTCTCCCACCCGGTGAAACAGGGGAGATATGCGTGCGAGGACCAAATATGATGAAAGGATATTTCAACAATCAGAAAGCCACCAGCCAAACCATAGACGATCAAGGATGGGTTCATACTGGAGATATCGGATACTTTAATGAAGAAGGAGAATTATTTGTTGTGGACAGAATCAAAGAACTCATCAAGTGTTATGGTTATCAGGTTGCACCAGCGGAGCTTGAAGCACTGCTACTTTCCCATCCTCAAATAATAGATGCCATTGTGATCCCATATCCTGATGATAAAGCCGGTGAGGTCCCTATAGCATTTGTTGTTGCACCATTCAAGAGATTAAAAATAGTAACCTTCACGAGTAGCGTTCCGAAGTCGGCTTCGGGAAAGCTTCTGAGAAGGGAGCTCATAGCACAAGTCCGTGCCAAGATGTAG

Protein sequence

MAIAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVANCNPKLVITVPELWDVIGKLNLPSLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFNEEGELFVVDRIKELIKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDDKAGEVPIAFVVAPFKRLKIVTFTSSVPKSASGKLLRRELIAQVRAKM

Homology

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BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot
Match: M4IQS1 (Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 1.9e-146
Identity = 284/543 (52.30%), Postives = 351/543 (64.64%), Query Frame = 0


Query: 7   MAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQL 66
           M K FNP++QIY+S RPP++FPTDP +S+ SFLFR+S+S+ +  AL DADSG++LTF +L
Sbjct: 1   MEKCFNPETQIYSSHRPPVNFPTDPKLSLTSFLFRSSASYPNRTALIDADSGQTLTFLKL 60

Query: 67  QIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSK 126
           + QVS+LAH+ + L I+K DVVLIFAPNSIHFPVCFF+I A+GAI TTCNP+YTF ELS 
Sbjct: 61  KTQVSKLAHSLVQLNIKKNDVVLIFAPNSIHFPVCFFSIAALGAITTTCNPSYTFTELSN 120

Query: 127 QVANCNPKLVITVPELWDVIGKLNLPS--------------------------------- 186
           Q  +CNP LVITVPELW+   KLNLP+                                 
Sbjct: 121 QAKDCNPTLVITVPELWEKARKLNLPAIILPSPSNSKLSSKSEFWFFSDLTRKSDRFSEL 180

Query: 187 ------------------------------------------------------------ 246
                                                                       
Sbjct: 181 PISDVRQSDVAALLYSSGTTGKSKGVVLSHKNFITTSLMVTSDQDRYGDPQNICMCFLPM 240

Query: 247 -------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS-VV 306
                        L+RGN VVSM KFELE AL  V  YR+THL++VPPV+IA+AK+S VV
Sbjct: 241 FHIFGLSVIAYSQLRRGNGVVSMGKFELEGALRAVEMYRVTHLFVVPPVMIALAKESVVV 300

Query: 307 KRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLS 366
           +RYDLSS+K+ILSGAAPLGK+VME+C++ +P   I QGYGMTETCG+IS+E+     R S
Sbjct: 301 RRYDLSSVKEILSGAAPLGKNVMEQCARNVPGAAIIQGYGMTETCGIISIEDSKKGCRFS 360

Query: 367 GSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 423
           GSTG L  GIE+QI+ T+    LPP + GEI +RGPNMM+GY NN +AT  TID+QGWV 
Sbjct: 361 GSTGMLAPGIESQIMDTKRWNPLPPNQEGEIWLRGPNMMQGYLNNLEATKSTIDEQGWVK 420

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BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot
Match: M4IRL6 (Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 4.6e-132
Identity = 261/544 (47.98%), Postives = 335/544 (61.58%), Query Frame = 0


Query: 7   MAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQL 66
           M KS   +  ++ S RPP+  P D  +S+VSF+FRNSSS+    AL D+D+ E+L+F Q 
Sbjct: 1   MEKSGYGRDGVFRSLRPPLVLPKDHNLSMVSFVFRNSSSYPQKPALIDSDTNETLSFSQF 60

Query: 67  QIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSK 126
           +  V +++H F++LG++K DVVLIFAPNSIH PVCF  IVA GAIATT NP YT +ELSK
Sbjct: 61  KSMVIKVSHGFLNLGVQKNDVVLIFAPNSIHVPVCFLGIVASGAIATTSNPLYTVSELSK 120

Query: 127 QVANCNPKLVITVPELWDVIGKLNLPS--------------------------------- 186
           QV + NPKL++TVPEL++ +   NLP+                                 
Sbjct: 121 QVKDSNPKLIVTVPELFEKVKGFNLPTILIGPNSEDSSPLKSRAKVLTFHDLVTLSGPVS 180

Query: 187 ------------------------------------------------------------ 246
                                                                       
Sbjct: 181 DFPMVDFKQSDTAALLYSSGTTGMSKGVVLSHKNFIASSLMVTMEQDQAGEMHNVFLCFL 240

Query: 247 ---------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 306
                          LQRGNTV+SMA+F+LEK L  V KY++THL++VPPVI+A+ K S+
Sbjct: 241 PMFHVFGLAIITYAQLQRGNTVISMARFDLEKILKDVEKYKVTHLWVVPPVILALTKNSI 300

Query: 307 VKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRL 366
           VK+YDLSSLK I SGAAPLGKD+MEEC+K++P   + QGYGMTETCG++S+E+     R 
Sbjct: 301 VKKYDLSSLKHIGSGAAPLGKDLMEECAKIVPHGIVAQGYGMTETCGIVSVEDTRGGKRH 360

Query: 367 SGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 423
           +GS G L SG+EAQI+S +T K LPP + GEI VRGPNMM+GYFNN +AT  TID +GWV
Sbjct: 361 TGSAGMLSSGVEAQIVSVDTLKPLPPNQLGEIWVRGPNMMQGYFNNPRATKLTIDKKGWV 420

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BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot
Match: Q9M0X9 (4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.9e-130
Identity = 260/544 (47.79%), Postives = 328/544 (60.29%), Query Frame = 0


Query: 7   MAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQL 66
           M KS   +  IY S RP +  P DP  S+VSFLFRNSSS+   LA+ D+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 67  QIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSK 126
           +  V++LAH F  LGIRK DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 127 QVANCNPKLVITVPELWDVIGKLNLP---------------------------------- 186
           Q+ + NPK++I+V +L+D I   +LP                                  
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 187 ------------------------------------------------------------ 246
                                                                       
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 247 --------------SLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 306
                          LQRGN +VSMA+FELE  L  + K+R+THL++VPPV +A++KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 307 VKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRL 366
           VK++DLSSLK I SGAAPLGKD+MEEC + +P   + QGYGMTETCG++S+E+  +  R 
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 367 SGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 423
           SGS G L  G+EAQI+S ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

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BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot
Match: Q0DV32 (4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1 PE=2 SV=2)

HSP 1 Score: 387.9 bits (995), Expect = 1.5e-106
Identity = 233/536 (43.47%), Postives = 301/536 (56.16%), Query Frame = 0


Query: 17  IYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADS-GESLTFRQLQIQVSQLAH 76
           +Y S RPP    +DP +S+   L R + +    +AL DA + G +LTF +L+  V   A 
Sbjct: 16  VYRSLRPPAPVASDPGLSLTDLLLRRADACPSAVALADAAAGGRALTFAELRSAVLSTAV 75

Query: 77  AF-IHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVANCNPK 136
           A     G+R GD VL+ APN + +PVCFFA+ A+GA+ TT NP YT  E++KQV++   K
Sbjct: 76  ALSSRAGVRPGDAVLLLAPNCVLYPVCFFAVTALGAVGTTVNPDYTPREIAKQVSDARAK 135

Query: 137 LVITVPELWDVIGKLNLP------------------------------------------ 196
           LVIT+  L   I  L LP                                          
Sbjct: 136 LVITISALVPKIAGLRLPVILLDDDANAAAASLPPDATVTLYTNLVAGVKEADYRRPPIK 195

Query: 197 ------------------------------------------------------------ 256
                                                                       
Sbjct: 196 QSDTAALLYSSGTTGDSKGVILTHRNFIAAARMVTSDQDERREGPNVFLCFLPMFHIFGL 255

Query: 257 ------SLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVVKRYDLSS 316
                  L RGN +++M++F++   +  V ++R+THL+ VPPVIIA+AK     +YDLSS
Sbjct: 256 SVITYAQLHRGNAIIAMSRFDINSLMEAVQRHRVTHLFCVPPVIIALAKHGKAGKYDLSS 315

Query: 317 LKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISME--NLGVESRLSGSTGF 376
           LK I SGAAPLGKDVME  +K  P ++I QGYGMTETCG+IS+E    G ++R  GSTG 
Sbjct: 316 LKFIGSGAAPLGKDVMEVVAKKFPDSEIVQGYGMTETCGIISLEYPEKG-QAREFGSTGT 375

Query: 377 LVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIG 421
           LVSG+EA+I+  +T K LPP + GEICVRGPN+M+GYFNN +AT  TI  QGW+HTGD+G
Sbjct: 376 LVSGVEAKIVDIKTLKHLPPNQVGEICVRGPNVMQGYFNNVQATEFTI-KQGWLHTGDLG 435

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BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot
Match: M4IQR7 (Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.9e-87
Identity = 194/535 (36.26%), Postives = 283/535 (52.90%), Query Frame = 0


Query: 15  SQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQLQIQVSQLA 74
           + I+ S R P+  P + ++ + +F+  +S +    +A  DA +G  LTF QL   V  +A
Sbjct: 19  NSIFYSKREPVQLPQNHSVDVTTFI--SSRAHHGKIAFIDAATGRHLTFPQLWRAVDSVA 78

Query: 75  HAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVANCNPK 134
                +GIRKGDV+L+ +PNSI+FPV   A++++GAI TT NP  T  E++KQ+ +  P 
Sbjct: 79  TCLSAMGIRKGDVILLLSPNSIYFPVVCLAVMSLGAIITTTNPLNTPREIAKQITDSKPV 138

Query: 135 LVITVPELWDVIGKLNLP------------------------------------------ 194
           L  T+P+L   I   NLP                                          
Sbjct: 139 LAFTIPQLVSKIAGSNLPIVIIDDEVKSSLEKTLNIVSSLGEMMRKEPSPNRIGYRVNQE 198

Query: 195 ------------------------------------------------------------ 254
                                                                       
Sbjct: 199 DTATLLYSSGTTGASKGVVSSHKNLIAMVQTILSRFGTEDGEHTFICTVPMFHIYGLAAF 258

Query: 255 ---SLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS--VVKRYDLSSL 314
               L  G+T+V ++KFE+ + L  + KYR T+L LVPP+++A+ K +  +  +YDLSSL
Sbjct: 259 AMGLLSSGSTIVILSKFEIHEMLSAIEKYRATYLPLVPPILMALLKNANHIRAKYDLSSL 318

Query: 315 KQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLSGSTGFLVS 374
           + +LSG APL K+V+E   +  P   I QGYG+TE+ G+ +  +   ESR  G+ G L  
Sbjct: 319 QSVLSGGAPLSKEVIEGFVENYPTVSILQGYGLTESTGIGASTDCLQESRRYGTAGMLSP 378

Query: 375 GIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYFN 423
            +EA+I++ ET + L    TGE+ +RGP +MKGYF+N++ATS TID +GW+ TGD+ Y +
Sbjct: 379 SMEAKIVNPETGEALSVNRTGELWLRGPTIMKGYFSNEEATSSTIDSEGWLRTGDLCYID 438

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BLAST of Cp4.1LG03g01660 vs. NCBI nr
Match: XP_023526195.1 (4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 769 bits (1985), Expect = 5.23e-277
Identity = 422/547 (77.15%), Postives = 422/547 (77.15%), Query Frame = 0


Query: 1   MAIAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGES 60
           MAIAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGES
Sbjct: 1   MAIAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGES 60

Query: 61  LTFRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYT 120
           LTFRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYT
Sbjct: 61  LTFRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYT 120

Query: 121 FAELSKQVANCNPKLVITVPELWDVIGKLNLPS--------------------------- 180
           FAELSKQVANCNPKLVITVPELWDVIGKLNLPS                           
Sbjct: 121 FAELSKQVANCNPKLVITVPELWDVIGKLNLPSVILGSKISSESSRPNIWVYSDLIKNSG 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 DVSDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFITTSLMVTHDQDLLGDPRNVFL 240

Query: 241 ------------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAK 300
                             LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAK
Sbjct: 241 CFLPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAK 300

Query: 301 QSVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVE 360
           QSVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVE
Sbjct: 301 QSVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVE 360

Query: 361 SRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQ 420
           SRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQ
Sbjct: 361 SRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQ 420

Query: 421 GWVHTGDIGYFNEEGELFVVDRIKELIKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDD 422
           GWVHTGDIGYFNEEGELFVVDRIKELIKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDD
Sbjct: 421 GWVHTGDIGYFNEEGELFVVDRIKELIKCYGYQVAPAELEALLLSHPQIIDAIVIPYPDD 480

[Close]

BLAST of Cp4.1LG03g01660 vs. NCBI nr
Match: XP_022982901.1 (4-coumarate--CoA ligase-like 7 [Cucurbita maxima])

HSP 1 Score: 751 bits (1939), Expect = 4.88e-270
Identity = 409/545 (75.05%), Postives = 418/545 (76.70%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIAMAKSFNPQSQIYTSPRPPIHFPTDPTISI+SFLFRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           E+SKQVA+CNPKLVIT+PELWDVIGKLNLPS                             
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 362
           VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 363 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 422
           LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

[Close]

BLAST of Cp4.1LG03g01660 vs. NCBI nr
Match: XP_022934454.1 (4-coumarate--CoA ligase-like 7 [Cucurbita moschata])

HSP 1 Score: 749 bits (1934), Expect = 2.81e-269
Identity = 409/545 (75.05%), Postives = 417/545 (76.51%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIAMAKSF+PQSQIYTSPRPPIHFPTDPTISIVSF+FRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS                             
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 362
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 363 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 422
           LSGSTG LVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGLLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

[Close]

BLAST of Cp4.1LG03g01660 vs. NCBI nr
Match: KAG6580619.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 745 bits (1924), Expect = 9.36e-268
Identity = 408/545 (74.86%), Postives = 416/545 (76.33%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIA+AK F+PQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAVAKYFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS                             
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 362
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 363 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 422
           LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

[Close]

BLAST of Cp4.1LG03g01660 vs. NCBI nr
Match: KAG7017376.1 (4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 732 bits (1890), Expect = 5.16e-262
Identity = 411/581 (70.74%), Postives = 417/581 (71.77%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIAMAKSF+PQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS                             
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ--------------------- 362
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQ                     
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQVLNCSFSTPPFCTFIWLLQEM 360

Query: 363 ---------------GYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 422
                          GYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR
Sbjct: 361 LYDVVLIWVAIYGVQGYGMTETCGVISMENLGVESRLSGSTGFLVSGIEAQILSTETQKR 420

[Close]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL
Match: A0A6J1J0P0 (4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 SV=1)

HSP 1 Score: 751 bits (1939), Expect = 2.36e-270
Identity = 409/545 (75.05%), Postives = 418/545 (76.70%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIAMAKSFNPQSQIYTSPRPPIHFPTDPTISI+SFLFRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISILSFLFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           E+SKQVA+CNPKLVIT+PELWDVIGKLNLPS                             
Sbjct: 121 EISKQVASCNPKLVITIPELWDVIGKLNLPSIILGSKISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDLPVSEVGQSDVAALLYSSGTTGINKGVILTHRNFITTSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 362
           VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR
Sbjct: 301 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 363 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 422
           LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

[Close]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL
Match: A0A6J1F2M7 (4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=4 SV=1)

HSP 1 Score: 749 bits (1934), Expect = 1.36e-269
Identity = 409/545 (75.05%), Postives = 417/545 (76.51%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +AIAMAKSF+PQSQIYTSPRPPIHFPTDPTISIVSF+FRNSSSFLH+LAL DADSGESLT
Sbjct: 1   MAIAMAKSFDPQSQIYTSPRPPIHFPTDPTISIVSFVFRNSSSFLHSLALADADSGESLT 60

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           FRQLQIQVS+LAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTF 
Sbjct: 61  FRQLQIQVSKLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFV 120

Query: 123 ELSKQVANCNPKLVITVPELWDVIGKLNLPS----------------------------- 182
           ELSKQVANCNPKLVITVPELWDVIGKLNLPS                             
Sbjct: 121 ELSKQVANCNPKLVITVPELWDVIGKLNLPSIILGSEISSKSSRPNIWVYSDLIKNSGDV 180

Query: 183 ------------------------------------------------------------ 242
                                                                       
Sbjct: 181 SDFPVSEVGQSDVAALLYSSGTTGISKGVILTHRNFIATSLMVTHDQDLLGDPRNVFLCF 240

Query: 243 ----------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 302
                           LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS
Sbjct: 241 LPMFHVFGLSVILYSQLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS 300

Query: 303 VVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 362
           VVK+YDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR
Sbjct: 301 VVKKYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESR 360

Query: 363 LSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 422
           LSGSTG LVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW
Sbjct: 361 LSGSTGLLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGW 420

[Close]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL
Match: A0A0A0LDK0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1)

HSP 1 Score: 683 bits (1762), Expect = 2.01e-243
Identity = 373/543 (68.69%), Postives = 396/543 (72.93%), Query Frame = 0


Query: 5   IAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFR 64
           I M KSFNPQSQ+YTS RPPIHFPTDPTISIVSFLFRNSSS+ + LAL DADSGESLTFR
Sbjct: 3   ITMNKSFNPQSQVYTSRRPPIHFPTDPTISIVSFLFRNSSSYPNALALVDADSGESLTFR 62

Query: 65  QLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAEL 124
           QLQIQVS+LAH FI LGI+KGDVVLIF+PNSIHF VCFFAIVAIGAIATTCNPAYT AEL
Sbjct: 63  QLQIQVSKLAHVFIQLGIQKGDVVLIFSPNSIHFLVCFFAIVAIGAIATTCNPAYTSAEL 122

Query: 125 SKQVANCNPKLVITVPELWDVIGKLNLPS------------------------------- 184
           SKQVANC PKLVITVPELWDVIGKLNLPS                               
Sbjct: 123 SKQVANCKPKLVITVPELWDVIGKLNLPSIILGSKISSKFSRSNIWGYSDLIKKAGDVSN 182

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 183 LPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITASLMVTQDQELLGDPRNVFLCFLP 242

Query: 245 --------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVV 304
                         LQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ VV
Sbjct: 243 MFHVFGLSIVVNSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVV 302

Query: 305 KRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLS 364
           K YDLSSL+QILSGAAPLGKDVM+ECSK++PQ +I QGYGMTETCGVIS+EN+GVES  S
Sbjct: 303 KNYDLSSLRQILSGAAPLGKDVMDECSKIIPQARIIQGYGMTETCGVISVENVGVESTQS 362

Query: 365 GSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422
           G+TG LVSG+EAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH
Sbjct: 363 GATGSLVSGVEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422

[Close]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL
Match: A0A5A7TKZ4 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002730 PE=4 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 1.64e-242
Identity = 374/543 (68.88%), Postives = 395/543 (72.74%), Query Frame = 0


Query: 5   IAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFR 64
           I M KSFNPQS+IY+SPRPPIHFPTDP ISIVSFLFRNSSS+ + LAL DADSGESLTFR
Sbjct: 3   ITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLTFR 62

Query: 65  QLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAEL 124
           QLQIQVS+LA  F  LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTFAEL
Sbjct: 63  QLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFAEL 122

Query: 125 SKQVANCNPKLVITVPELWDVIGKLNLPS------------------------------- 184
           SKQVANCNPKLVITVPELWDVIGKLNLPS                               
Sbjct: 123 SKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDVSN 182

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 183 LPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCFLP 242

Query: 245 --------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVV 304
                         LQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ VV
Sbjct: 243 MFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVV 302

Query: 305 KRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLS 364
           K YDLSSL+QILSGAAPLGKDVMEECSK++PQ +I QGYGMTETCGVIS+EN+ VES LS
Sbjct: 303 KNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVESTLS 362

Query: 365 GSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422
           G+TG L SGIEAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH
Sbjct: 363 GATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422

[Close]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL
Match: A0A1S3B5U8 (4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 1.64e-242
Identity = 374/543 (68.88%), Postives = 395/543 (72.74%), Query Frame = 0


Query: 5   IAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFR 64
           I M KSFNPQS+IY+SPRPPIHFPTDP ISIVSFLFRNSSS+ + LAL DADSGESLTFR
Sbjct: 3   ITMNKSFNPQSRIYSSPRPPIHFPTDPKISIVSFLFRNSSSYPNALALADADSGESLTFR 62

Query: 65  QLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAEL 124
           QLQIQVS+LA  F  LGI+KGDVVLIFAPNSIHF VCFFAIVAIGAIATTCNPAYTFAEL
Sbjct: 63  QLQIQVSKLARVFSQLGIQKGDVVLIFAPNSIHFLVCFFAIVAIGAIATTCNPAYTFAEL 122

Query: 125 SKQVANCNPKLVITVPELWDVIGKLNLPS------------------------------- 184
           SKQVANCNPKLVITVPELWDVIGKLNLPS                               
Sbjct: 123 SKQVANCNPKLVITVPELWDVIGKLNLPSMILGSKISSKFSRSNIWSYSDLIKKAGDVSN 182

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 183 LPVSEVGQNDVAALLYSSGTTGISKGVILTHRNFITTSLMVTQDQELLGDPRNVFLCFLP 242

Query: 245 --------------LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSVV 304
                         LQRGNTVVSMAKFELEKALG+VMKY+ITHLY+VPPVIIA+ KQ VV
Sbjct: 243 MFHVFGLSVIVYSQLQRGNTVVSMAKFELEKALGLVMKYKITHLYVVPPVIIALTKQKVV 302

Query: 305 KRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLS 364
           K YDLSSL+QILSGAAPLGKDVMEECSK++PQ +I QGYGMTETCGVIS+EN+ VES LS
Sbjct: 303 KNYDLSSLRQILSGAAPLGKDVMEECSKIIPQARIIQGYGMTETCGVISVENMVVESTLS 362

Query: 365 GSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422
           G+TG L SGIEAQILS ETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH
Sbjct: 363 GATGSLGSGIEAQILSIETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVH 422

[Close]

BLAST of Cp4.1LG03g01660 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 467.2 bits (1201), Expect = 1.4e-131
Identity = 260/544 (47.79%), Postives = 328/544 (60.29%), Query Frame = 0


Query: 7   MAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQL 66
           M KS   +  IY S RP +  P DP  S+VSFLFRNSSS+   LA+ D+D+G+SLTF QL
Sbjct: 1   MEKSGYGRDGIYRSLRPTLVLPKDPNTSLVSFLFRNSSSYPSKLAIADSDTGDSLTFSQL 60

Query: 67  QIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSK 126
           +  V++LAH F  LGIRK DVVLIFAPNS  FP+CF A+ AIG + TT NP YT  E+SK
Sbjct: 61  KSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVTAIGGVFTTANPLYTVNEVSK 120

Query: 127 QVANCNPKLVITVPELWDVIGKLNLP---------------------------------- 186
           Q+ + NPK++I+V +L+D I   +LP                                  
Sbjct: 121 QIKDSNPKIIISVNQLFDKIKGFDLPVVLLGSKDTVEIPPGSNSKILSFDNVMELSEPVS 180

Query: 187 ------------------------------------------------------------ 246
                                                                       
Sbjct: 181 EYPFVEIKQSDTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFL 240

Query: 247 --------------SLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV 306
                          LQRGN +VSMA+FELE  L  + K+R+THL++VPPV +A++KQS+
Sbjct: 241 PMFHVFGLAVITYSQLQRGNALVSMARFELELVLKNIEKFRVTHLWVVPPVFLALSKQSI 300

Query: 307 VKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRL 366
           VK++DLSSLK I SGAAPLGKD+MEEC + +P   + QGYGMTETCG++S+E+  +  R 
Sbjct: 301 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQGYGMTETCGIVSVEDPRLGKRN 360

Query: 367 SGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWV 423
           SGS G L  G+EAQI+S ET K  PP + GEI VRGPNMMKGY NN +AT +TID + WV
Sbjct: 361 SGSAGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYLNNPQATKETIDKKSWV 420

[Close]

BLAST of Cp4.1LG03g01660 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 303.1 bits (775), Expect = 3.4e-82
Identity = 186/529 (35.16%), Postives = 278/529 (52.55%), Query Frame = 0


Query: 12  NPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQLQIQVS 71
           N  S  Y S R PI  P +P++ + +F+  +S +    +A  DA +G++LTF +L   V 
Sbjct: 12  NSNSTFY-SKRTPIPLPPNPSLDVTTFI--SSQAHRGRIAFIDASTGQNLTFTELWRAVE 71

Query: 72  QLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVANC 131
            +A     +GIRKG VVL+ +PNSI FPV   +++++GAI TT NP  T  E++KQ+ + 
Sbjct: 72  SVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAIITTTNPLNTSNEIAKQIKDS 131

Query: 132 NPKLVITVPELW--------------------DVIGKLNL--------PS---------- 191
           NP L  T  +L                     D +G +          PS          
Sbjct: 132 NPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGDVRRLVEMMKKEPSGNRVKERVDQ 191

Query: 192 ------------------------------------------------------------ 251
                                                                       
Sbjct: 192 DDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAA 251

Query: 252 -----LQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAK--QSVVKRYDLSS 311
                L  G+T++ ++KFE+ + +  + KY+ T L LVPP+++AM      +  +YDLSS
Sbjct: 252 FATGLLAYGSTIIVLSKFEMHEMMSAIGKYQATSLPLVPPILVAMVNGADQIKAKYDLSS 311

Query: 312 LKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLSGSTGFLV 371
           +  +L G APL K+V E  ++  P  KI QGYG+TE+ G+ +  +   ESR  G+ G L 
Sbjct: 312 MHTVLCGGAPLSKEVTEGFAEKYPTVKILQGYGLTESTGIGASTDTVEESRRYGTAGKLS 371

Query: 372 SGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGYF 416
           + +E +I+   T + L P +TGE+ ++GP++MKGYF+N++ATS T+D +GW+ TGD+ Y 
Sbjct: 372 ASMEGRIVDPVTGQILGPKQTGELWLKGPSIMKGYFSNEEATSSTLDSEGWLRTGDLCYI 431

[Close]

BLAST of Cp4.1LG03g01660 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.3e-76
Identity = 179/549 (32.60%), Postives = 275/549 (50.09%), Query Frame = 0


Query: 3   IAIAMAKSFNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLT 62
           +A+     F   + I+ S R P+  P +  + + SF+   S          DA +G  L+
Sbjct: 19  LAVDRKSGFCESTSIFYSKREPMALPPNQFLDVTSFI--ASQPHRGKTVFVDAVTGRRLS 78

Query: 63  FRQLQIQVSQLAHAFIHLGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFA 122
           F +L + V ++A     LG+RKG+VV+I +PNSI FP+   +++++GAI TT NP  T  
Sbjct: 79  FPELWLGVERVAGCLYALGVRKGNVVIILSPNSILFPIVSLSVMSLGAIITTANPINTSD 138

Query: 123 ELSKQVANCNPKLVITVPELWD------------------------------VIGKLNL- 182
           E+SKQ+ +  P L  T  +L                                ++G+L   
Sbjct: 139 EISKQIGDSRPVLAFTTCKLVSKLAAASNFNLPVVLMDDYHVPSQSYGDRVKLVGRLETM 198

Query: 183 ----PSLQR--------------------------------------------------- 242
               PS  R                                                   
Sbjct: 199 IETEPSESRVKQRVNQDDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTI 258

Query: 243 ---------------------GNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAK 302
                                G T+V + KF++ K L  V  +R ++L LVPP+++AM  
Sbjct: 259 CTIPMCHIFGFGGFATGLIALGWTIVVLPKFDMAKLLSAVETHRSSYLSLVPPIVVAMVN 318

Query: 303 --QSVVKRYDLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLG 362
               +  +YDLSSL  +++G APL ++V E+  +  P+ KI QGYG+TE+  + +     
Sbjct: 319 GANEINSKYDLSSLHTVVAGGAPLSREVTEKFVENYPKVKILQGYGLTESTAIAASMFNK 378

Query: 363 VESRLSGSTGFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTID 422
            E++  G++G L   +E +I+  +T + L   +TGE+ +R P +MKGYF N++AT+ TID
Sbjct: 379 EETKRYGASGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYFKNKEATASTID 438

[Close]

BLAST of Cp4.1LG03g01660 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 283.9 bits (725), Expect = 2.1e-76
Identity = 187/537 (34.82%), Postives = 274/537 (51.02%), Query Frame = 0


Query: 11  FNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQLQIQV 70
           F   +  + S R P+  P + +  + +F+  +S       A  DA +G+ LTF  L   V
Sbjct: 16  FCKSNSTFYSKRQPLSLPPNLSRDVTTFI--SSQPHRGKTAFIDAATGQCLTFSDLWRAV 75

Query: 71  SQLAHAFIH-LGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVA 130
            ++A    H +GIR+GDVVLI +PNSI  PV   +++++GA+ TT N   T  E+SKQ+A
Sbjct: 76  DRVADCLYHEVGIRRGDVVLILSPNSIFIPVVCLSVMSLGAVFTTANTLNTSGEISKQIA 135

Query: 131 NCNPKLVITVPELWD------------------------VIGKLN-----LPSLQR---- 190
           + NP LV T  +L                          V+G L+      PS QR    
Sbjct: 136 DSNPTLVFTTRQLAPKLPVAISVVLTDDEVYQELTSAIRVVGILSEMVKKEPSGQRVRDR 195

Query: 191 ------------------------------------------------------------ 250
                                                                       
Sbjct: 196 VNQDDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISDNLKRDDIFICTVPMFHTYGLL 255

Query: 251 ---------GNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQS--VVKRYDLS 310
                    G+TVV + +F+L   +  V K+R T L L PPV++AM   +  +  +YDLS
Sbjct: 256 TFAMGTVALGSTVVILRRFQLHDMMDAVEKHRATALALAPPVLVAMINDADLIKAKYDLS 315

Query: 311 SLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLSGSTGFL 370
           SLK +  G APL K+V E   +  P   I QGY +TE+ G  +  N   ESR  G+ G L
Sbjct: 316 SLKTVRCGGAPLSKEVTEGFLEKYPTVDILQGYALTESNGGGAFTNSAEESRRYGTAGTL 375

Query: 371 VSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGDIGY 423
            S +EA+I+   T + +   +TGE+ ++GP++ KGYF NQ+AT++TI+ +GW+ TGD+ Y
Sbjct: 376 TSDVEARIVDPNTGRFMGINQTGELWLKGPSISKGYFKNQEATNETINLEGWLKTGDLCY 435

[Close]

BLAST of Cp4.1LG03g01660 vs. TAIR 10
Match: AT4G19010.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 277.3 bits (708), Expect = 2.0e-74
Identity = 175/532 (32.89%), Postives = 268/532 (50.38%), Query Frame = 0


Query: 11  FNPQSQIYTSPRPPIHFPTDPTISIVSFLFRNSSSFLHTLALTDADSGESLTFRQLQIQV 70
           F+ ++ IYTS  P +H P DP +  VS LF  S       AL D+ +G S++  +LQI V
Sbjct: 25  FSSKTGIYTSKFPSLHLPVDPNLDAVSALF--SHKHHGDTALIDSLTGFSISHTELQIMV 84

Query: 71  SQLAHAFIH-LGIRKGDVVLIFAPNSIHFPVCFFAIVAIGAIATTCNPAYTFAELSKQVA 130
             +A    H LG+R+GDVV +  PNS++FP+ F +++++GAI TT NP+ +  E+ KQV+
Sbjct: 85  QSMAAGIYHVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGAIVTTMNPSSSLGEIKKQVS 144

Query: 131 NCNPKL-----------------VITVPELWD---------------------------- 190
            C+  L                 VI+V E +D                            
Sbjct: 145 ECSVGLAFTSTENVEKLSSLGVSVISVSESYDFDSIRIENPKFYSIMKESFGFVPKPLIK 204

Query: 191 --------------------------VIGKLNL--------------------------- 250
                                     +I  + L                           
Sbjct: 205 QDDVAAIMYSSGTTGASKGVLLTHRNLIASMELFVRFEASQYEYPGSSNVYLAALPLCHI 264

Query: 251 --------PSLQRGNTVVSMAKFELEKALGVVMKYRITHLYLVPPVIIAMAKQSV-VKRY 310
                     L  G+T+V M +F+    + V+ +++ITH  +VPP+++A+ K++  V   
Sbjct: 265 YGLSLFVMGLLSLGSTIVVMKRFDASDVVNVIERFKITHFPVVPPMLMALTKKAKGVCGE 324

Query: 311 DLSSLKQILSGAAPLGKDVMEECSKVLPQTKITQGYGMTETCGVISMENLGVESRLSGST 370
              SLKQ+ SGAAPL +  +E+  + LP   + QGYGMTE+  V +      +     S 
Sbjct: 325 VFKSLKQVSSGAAPLSRKFIEDFLQTLPHVDLIQGYGMTESTAVGTRGFNSEKLSRYSSV 384

Query: 371 GFLVSGIEAQILSTETQKRLPPGETGEICVRGPNMMKGYFNNQKATSQTIDDQGWVHTGD 415
           G L   ++A+++   +   LPPG  GE+ ++GP +MKGY NN KAT  +I +  W+ TGD
Sbjct: 385 GLLAPNMQAKVVDWSSGSFLPPGNRGELWIQGPGVMKGYLNNPKATQMSIVEDSWLRTGD 444

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g01660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
M4IQS1	1.9e-146	52.30	Probable CoA ligase CCL10 OS=Humulus lupulus OX=3486 GN=CCL10 PE=2 SV=1	[more]
M4IRL6	4.6e-132	47.98	Probable CoA ligase CCL7 OS=Humulus lupulus OX=3486 GN=CCL7 PE=2 SV=1	[more]
Q9M0X9	1.9e-130	47.79	4-coumarate--CoA ligase-like 7 OS=Arabidopsis thaliana OX=3702 GN=4CLL7 PE=1 SV=...	[more]
Q0DV32	1.5e-106	43.47	4-coumarate--CoA ligase-like 1 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL1...	[more]
M4IQR7	5.9e-87	36.26	Probable CoA ligase CCL5 OS=Humulus lupulus OX=3486 GN=CCL5 PE=2 SV=1	[more]

BLAST of Cp4.1LG03g01660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023526195.1	5.23e-277	77.15	4-coumarate--CoA ligase-like 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022982901.1	4.88e-270	75.05	4-coumarate--CoA ligase-like 7 [Cucurbita maxima]	[more]
XP_022934454.1	2.81e-269	75.05	4-coumarate--CoA ligase-like 7 [Cucurbita moschata]	[more]
KAG6580619.1	9.36e-268	74.86	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7017376.1	5.16e-262	70.74	4-coumarate--CoA ligase-like 7, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of Cp4.1LG03g01660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J0P0	2.36e-270	75.05	4-coumarate--CoA ligase-like 7 OS=Cucurbita maxima OX=3661 GN=LOC111481607 PE=4 ...	[more]
A0A6J1F2M7	1.36e-269	75.05	4-coumarate--CoA ligase-like 7 OS=Cucurbita moschata OX=3662 GN=LOC111441628 PE=...	[more]
A0A0A0LDK0	2.01e-243	68.69	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G740270 PE=4 SV=1	[more]
A0A5A7TKZ4	1.64e-242	68.88	4-coumarate--CoA ligase-like 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B5U8	1.64e-242	68.88	4-coumarate--CoA ligase-like 7 OS=Cucumis melo OX=3656 GN=LOC103486502 PE=4 SV=1	[more]

BLAST of Cp4.1LG03g01660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G05160.1	1.4e-131	47.79	AMP-dependent synthetase and ligase family protein	[more]
AT1G20510.1	3.4e-82	35.16	OPC-8:0 CoA ligase1	[more]
AT1G20480.1	1.3e-76	32.60	AMP-dependent synthetase and ligase family protein	[more]
AT1G20500.1	2.1e-76	34.82	AMP-dependent synthetase and ligase family protein	[more]
AT4G19010.1	2.0e-74	32.89	AMP-dependent synthetase and ligase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.980		coord: 150..253 e-value: 4.9E-37 score: 129.1
None	No IPR available	GENE3D	2.30.38.10	Luciferase; Domain 3	coord: 263..337 e-value: 8.0E-31 score: 107.7
None	No IPR available	GENE3D	3.30.300.30		coord: 342..422 e-value: 4.0E-15 score: 58.0
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 154..386
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 10..154
None	No IPR available	PANTHER	PTHR24096:SF373	4-COUMARATE:COA LIGASE-LIKE PROTEIN	coord: 10..154
None	No IPR available	PANTHER	PTHR24096:SF373	4-COUMARATE:COA LIGASE-LIKE PROTEIN	coord: 154..386
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 17..420
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 353..421 e-value: 4.7E-7 score: 30.7
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 16..149 e-value: 1.9E-29 score: 104.6
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 49..138 e-value: 4.9E-22 score: 78.1 coord: 152..344 e-value: 2.7E-56 score: 191.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g01660.1	Cp4.1LG03g01660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016405	CoA-ligase activity