Cp4.1LG03g01570 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g01570
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCarbonic anhydrase
LocationCp4.1LG03: 1016876 .. 1022374 (+)
RNA-Seq ExpressionCp4.1LG03g01570
SyntenyCp4.1LG03g01570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAGAGGGAGCAAGAAAGGGCCAACGAGATGGGGCGAGATTAAAAAAGAATGGGCGATGTGCAACAACGGCGACATGCAATCACCCATTGATTTGTCAAGCAAAAGAGTCAAAGTTGTCAAAAGTTTAGGTCAACTTAAGAGGAAATACAAACCCTCCATGGCCATTCTCAAAAATCGAGGCCACGACATTACGGTAACGTTACATAAATTCACTATTGTTAAGGATAGTTGACCGTTCATTATAAGGCAAATATCTAGATACAAATATAATAAGTCGTAATGTAAGGCAAATATCTTATAATGAACGGTCAACTATCCTTAACAGTTATCCATCCTTCAACTTTATAATATATAATACAATATAATAATTGATCCAACAGATTAAGTGGGAGGGAGACGCAGGATCAATCGAGATCAACGGAACAAATTATGTTCTTCAGCAAGCTCATTGGCACTCGCTTTCTGAACACACCCTTAATGGAAGAAGCTACGATTTAGAGGTTCATTTGGTTCACCAAAGCTCAAACCCAGACGCCAAATATCCGATTGCGGTTGTTGGCGTGTTCTACAAGATTGGTCGTCCCGATGCGTTCCTGGGCAAGGTGACCGAATAATGAAAAGCATATAAAATTTAACGTGAATTTGATGGAATTTAAATTGAAGTATATATTGAAATGTTGATAGATGAGTAGAAAAATAAAGGAATTGGGTGAAGAAAAGGAGAAAAAAGCAGGAAGGATAGATCCTAGAGGAATCAAAATGGGTGGGGAGAAGTACTATAGATACATGGGATCCCTCACTGTCCCTCCCTGTACTGAGGGTGTCATTTGGAACATTAACAAAAAGGTATTATTATTATTATTATTTTTTTTTTTTTCAAATTAGGATATAAAAATAAAAATAATAATTATTATTATTGTTATCAGGTACAAACAGTCTCACGGGAACAAGTGAGCCTTCTGAGATCAGCGGTTCACGATGTAAGTATCAACTTATTAATTTTTTTATTTTTATTTTTTATAATTAAAACCATTTAATTATTTATTGTGAATTGCAGTCTGCAGAGAGGAATGCAAGACCAATCCAACCACATAATGGACGACACGTCGACCTTTATGATCCAAATCCTACGCACATAATTAATTGAAATTATATATATATATATATATATTATTATTTTTTCCTTTTAGGATATTTGAGAATTTAATTATTTCCTTTATTTATCACCTATTGTCTCATTTTTATCCCCAAATAATAATAATAATAATGTTGCTATTTTTCTTTTTATACAACTCATACTACACTTTTTTTCTTTTTTTATTTTCCACAAAATTAAAAATTTAAGAGTTGTAAGAAACATAAATTATAATTTATGTTTTAAAAAAATTAACCTCCAACTCANTTTTTTTTTTTTTTTTTTTTTTTTAATTAGACACGTGGGAAATTTGGAATGTTTGCAAGACTCATCTCCTTTCTAACAAGGAAACGTGGCAGCCTTTGCATTAAAAAATAATTAGTTGTAAAAAAAAAAATATATATATATCTAATTTAACCTTTAATTTTTAAAAAAATATAAATATTTAGGAAGTGTTACGATGGTTGGATGATGAAAGTCTCATATCATCCAACGTTGCTGTAAAAATCACATGAGAGTGTCAATAGATTGGTAAATCCTCAAATGTCAAACAAAGGACTACAGAAGAAAAGAAGTCAAGCCTCCTCTCGAATGCAATAAAAAATGATTAAGATTTCAAAGGAGTCGAGCCTAGATTAAGGGGAGGCGTACTTTGTTCGAGGGGAGGAGTTGGATCATGAAAGTCCCACCTCGGCTAATTTAGGGAATAATCATGGGTTTATAATCAAAGAATACTCTCTCCATCGTTATGAGGCTTTTTGGGGAGCCCAGCAAAGGCATGAGAGCTTTTACTTAAAGTTGACAATATCATACCATTGTGGAGAGTTATGTTCGTCTAACAACGAAGACACAACAATACACTTAATGAAAATATAATATAACTCCTCACCTCTCTTTAAAACCTAAGTGGGTGAAGATTAAAATATTTGGTTAAAAAAAGAAAGATGACACGTGGAAAGTTGAATAACTATAAACATAAAGTGTTATAATTTAAAATCAATGAGTAACTTTTTTTTAAAAAAAAAATAATAATAAAAAATAAAGTTGAACACGTTTTTGTTTTTATTTTAATTTTTATTATGTTTTCGAAAAAAAAAAACATTGAATGAGATGCAATTATTGAGTTACTTGTTGTTTTGATTTTGGAAGGAAAAAGAAAAGTTTGACACGAAAAAGTAATTTCGAAGTAATTGATAATTATCTGATTTAGATTATTACTAATTCCGTTTTGAATGTTTTAAAGGTTAAAAATTATATTTTTAAAGGCTAAATATTTGTATTTTATTTTATTTAAGTATCCAACAAAAGTTGATATTGGCGTGGGAGTTGGTTCACCTGCCCCTATATTTTCACTTTATCGATGGAATTTATGTAAATTTAAAAACACACACGTTTCATATTTATAGCATTTTATAATACAATAATTTATTAATGATAGTATCCATAATTTGAAAAGTTAAATTCCCTACTCATACGAAACGTCCCAAATCAAAACAAAATATTTTTTTATAAACGTATGAAAACCATTTTTTACCAAACACGTTTTAAAATCGTGAAATTGACGATAGGCTTCAATTGTTACCGATGGTATTAGAGTCAGACACTATCGGACAATGTACCAGCAAGGACACTGAGTTTCAAGGGGGTGTGGATTGTGAGATCCCATATTGGTTGAAGAAGGAAACAAAGGGGTGCCAGCGAGAACGTTGAGTCACAAGGGGGGTGGATTGTGAGATCTCACATTGGTTGGAGAAGGGAACGAAACATTTCTCGTAAAAGTGTGAGAACGTCTTCTTAACAGACGCGTGAGACTGATGTTGATACGTAAAAAACGAAACATTGCCTATCTTAGAAAATAGGCGATGAGCTTAAAGTGTTACAAATGGTATCAGAGTCAGACAACGTCGGGAAGTGTGCCAACAAGGACGATGAGTTTCAAGGGGGTGGACTGTGAGATCACACGTTGGTTGGAGAGAGAAGAGAAACGTAGGGTCTCAGGGGGGTGGATTGTGAAATCACACATTGTTTAGAGAAGGGAATGAAACATTTCTTGTAAAGGCGTGGAAACCTCTCCCTAACAAACGTGTGAAACTGACAACGATACGTAACGGGCTAAAGCGAACGTCGTGTGATGATGATTTTTGTGCATTAGATTCTTTTTTCATATGTTTTAGTAATTATTTTCTAAAAGCTACAATAAAGTATTGATCTTTTTAAAAAAAACATGGAATAATATTCCTCCCAACCACTCATCCAATAGGGTTTGAGTAGGATAAAGGTTGATGGAATATGATAGTTTTCAGACTTGGAATCCATGCACAAGAAAATAGAATATAAGTGGGTGGGATGGGCACATCATTTTTGTAACCATTTTCCATTTACATAGCTCTAAAGAAATGGAAGCCTCCCATCTTCTCATATTTGCTATCTGTTGCTTATCATTGCTATCAAGTGGAGCAGCAGCAGCATCAGCAGCGAAGGTCGGTGGGGAGTTCAGCTATGATCCATGTAGCGACAGAGGGCCTAAGCATTGGGGAGAATTGGATATGGATTGGGGAATGTGTAAGAATGGCAAACTCCAGTCCCCTATTTCCTTGTCAAAATGGACTGCTGATTTAAACCAGGGCTTGGGTGATCTTAGACGAAGACATAGACCTGCTAATGCCTATCTCAAGAACGATGGCCACGAAATTGTTGTAAGTATTGCTTTTCTCCTAACTCGGATCTTCACAAGGAGCATAAACTCTCGTGGCTTTACTTTAAGCTTCCCCAAACGGCTTCATACCAATAGAGATCTATTTCTTACTTATGAACTCATAGCATCGAAGCTTCAATTAAAGGGAGGCTGTTCAAGGACTTCATAGGTCTCAAAAGAGACTCTATAGTGTACTTTGTTCGAGGGGAGGATTGTTGACGATGGTTGGGAAGGAGTCTTACATTGGCTAATTTAGGGAATGATCATGCGTTTATAAGTAAGAAATACATCTTTATCGGTCTTACCACTTGTCACAATCGCACTCTTAATGGCAGCGGACTTGCGATTGTGCGGCACTCACTTTCCAACGAAAAGTGAGCTAAGCCAATTCGTTCTTACGTCGCCTACGGCCAAACGGCGTATGCTCGAGCCTCTGGCCTCGGTTTAAGAAGAAGGTTTTAGAAAACGAGAGCAGAGAGAGTTTGTGAAAGAGACGTTATATATGCAAGCAAGAGAGTAACAACAACTGCTCACAGTATATAATTTTTTTGTAGGGAGGGGGAAAATCCAAATGAAAGTCACGAGAGCTTATGTTCAAAGAGGACAGTATATAATATTGTGGAGATTCGTGATTCTTAACATCCTCACCCACCAAGAAAAGCACACGTTTTTGGAATATTTCTATAATTAATTGACTACCCATCACTCTAATACGTGTTTTTATTGTTTTTATAATCCAAAGTTTACAACTTTTTTGTAAAACGCAGGTAGATTGGGACGGAAATGATGCTGGATCAATCAAGATCAAGAACATTGATTACCAGCTTGTAAATTACCATTGGCATCATCCTTCCGAGCATACCCAAGATGGCAAAACGTTTCCTTTGGAGCTTCACATGGTCCACATCAACAACGTAACAAAGAACATAGCTGTTATTGGCATTTTATACGAATACGGACCACTCGATCCATTCATCTTCATGGTAAAATCATCCACAAATTCCTTATTATTACACAAAATCATTGAAATTCATATGTTCTTGTTGAATTTCAGTTAGAAAGGGACATACAAAATCTAGATATTGAAGGAGGCTTGGAATTTCTTGGGCAAGTGGATCCTAGAGACGTAAAGCTTGGTGGCAGGAAATACTTTAGATACATGGGATCTCTGACCACTCCTCCGTGTACTGAAGGTGTCACTTGGACTGTCATGGACAAGGTTCATACTTTGCATTTGATACTCATTCATCCCTTTCATGGGTTGATGATGCTGATATTGATACTCATTTTTTAGGTACAAACTGTGGCACCTTATCAAGTCCGGCTTCTTAAACGAGCTTTGGTTGAAGTAAGTTCTGTTCCAACCCTATTAAAATGTCCTCGAATTTAGTGACAGTTTGGATGAGAATTGAAATTAATGGTTTGTCGTTTTTTGTTGCAGGAGAAGAATGCGAGGCCTTTGCAAAAGGTCAACGGTAGAGATGTCTCCTACTTTGACCCATACTCGTGGGTGGGAAGTGTAGCAGCTGAGTAGACGTTTCTGCCCAATTCTTAACTGCCACGTATTACTTAGAATAAAAGACCATGGGATTTTGTCAAATCCTATGTTTATTAAATGCTTTGGATAATGTTTCTGAAATTTCAATAATCAATAATTGTGACTTTGT

mRNA sequence

ATAAGAGGGAGCAAGAAAGGGCCAACGAGATGGGGCGAGATTAAAAAAGAATGGGCGATGTGCAACAACGGCGACATGCAATCACCCATTGATTTGTCAAGCAAAAGAGTCAAAGTTGTCAAAAGTTTAGGTCAACTTAAGAGGAAATACAAACCCTCCATGGCCATTCTCAAAAATCGAGGCCACGACATTACGATTAAGTGGGAGGGAGACGCAGGATCAATCGAGATCAACGGAACAAATTATGTTCTTCAGCAAGCTCATTGGCACTCGCTTTCTGAACACACCCTTAATGGAAGAAGCTACGATTTAGAGGTTCATTTGGTTCACCAAAGCTCAAACCCAGACGCCAAATATCCGATTGCGGTTGTTGGCGTGTTCTACAAGATTGGTCGTCCCGATGCGTTCCTGGGCAAGATGAGTAGAAAAATAAAGGAATTGGGTGAAGAAAAGGAGAAAAAAGCAGGAAGGATAGATCCTAGAGGAATCAAAATGGGTGGGGAGAAGTACTATAGATACATGGGATCCCTCACTGTCCCTCCCTGTACTGAGGGTGTCATTTGGAACATTAACAAAAAGGTACAAACAGTCTCACGGGAACAAGTGAGCCTTCTGAGATCAGCGGTTCACGATAGAGGAATGCAAGACCAATCCAACCACATAATGGACGACACCTCTAAAGAAATGGAAGCCTCCCATCTTCTCATATTTGCTATCTGTTGCTTATCATTGCTATCAAGTGGAGCAGCAGCAGCATCAGCAGCGAAGGTCGGTGGGGAGTTCAGCTATGATCCATGTAGCGACAGAGGGCCTAAGCATTGGGGAGAATTGGATATGGATTGGGGAATGTGTAAGAATGGCAAACTCCAGTCCCCTATTTCCTTGTCAAAATGGACTGCTGATTTAAACCAGGGCTTGGATTGGGACGGAAATGATGCTGGATCAATCAAGATCAAGAACATTGATTACCAGCTTGTAAATTACCATTGGCATCATCCTTCCGAGCATACCCAAGATGGCAAAACGTTTCCTTTGGAGCTTCACATGGTCCACATCAACAACGTAACAAAGAACATAGCTGTTATTGGCATTTTATACGAATACGGACCACTCGATCCATTCATCTTCATGTTAGAAAGGGACATACAAAATCTAGATATTGAAGGAGGCTTGGAATTTCTTGGGCAAGTGGATCCTAGAGACGTAAAGCTTGGTGGCAGGAAATACTTTAGATACATGGGATCTCTGACCACTCCTCCGTGTACTGAAGGTGTCACTTGGACTGTCATGGACAAGGTACAAACTGTGGCACCTTATCAAGTCCGGCTTCTTAAACGAGCTTTGGTTGAAGAGAAGAATGCGAGGCCTTTGCAAAAGGTCAACGGTAGAGATGTCTCCTACTTTGACCCATACTCGTGGGTGGGAAGTGTAGCAGCTGAGTAGACGTTTCTGCCCAATTCTTAACTGCCACGTATTACTTAGAATAAAAGACCATGGGATTTTGTCAAATCCTATGTTTATTAAATGCTTTGGATAATGTTTCTGAAATTTCAATAATCAATAATTGTGACTTTGT

Coding sequence (CDS)

ATGTGCAACAACGGCGACATGCAATCACCCATTGATTTGTCAAGCAAAAGAGTCAAAGTTGTCAAAAGTTTAGGTCAACTTAAGAGGAAATACAAACCCTCCATGGCCATTCTCAAAAATCGAGGCCACGACATTACGATTAAGTGGGAGGGAGACGCAGGATCAATCGAGATCAACGGAACAAATTATGTTCTTCAGCAAGCTCATTGGCACTCGCTTTCTGAACACACCCTTAATGGAAGAAGCTACGATTTAGAGGTTCATTTGGTTCACCAAAGCTCAAACCCAGACGCCAAATATCCGATTGCGGTTGTTGGCGTGTTCTACAAGATTGGTCGTCCCGATGCGTTCCTGGGCAAGATGAGTAGAAAAATAAAGGAATTGGGTGAAGAAAAGGAGAAAAAAGCAGGAAGGATAGATCCTAGAGGAATCAAAATGGGTGGGGAGAAGTACTATAGATACATGGGATCCCTCACTGTCCCTCCCTGTACTGAGGGTGTCATTTGGAACATTAACAAAAAGGTACAAACAGTCTCACGGGAACAAGTGAGCCTTCTGAGATCAGCGGTTCACGATAGAGGAATGCAAGACCAATCCAACCACATAATGGACGACACCTCTAAAGAAATGGAAGCCTCCCATCTTCTCATATTTGCTATCTGTTGCTTATCATTGCTATCAAGTGGAGCAGCAGCAGCATCAGCAGCGAAGGTCGGTGGGGAGTTCAGCTATGATCCATGTAGCGACAGAGGGCCTAAGCATTGGGGAGAATTGGATATGGATTGGGGAATGTGTAAGAATGGCAAACTCCAGTCCCCTATTTCCTTGTCAAAATGGACTGCTGATTTAAACCAGGGCTTGGATTGGGACGGAAATGATGCTGGATCAATCAAGATCAAGAACATTGATTACCAGCTTGTAAATTACCATTGGCATCATCCTTCCGAGCATACCCAAGATGGCAAAACGTTTCCTTTGGAGCTTCACATGGTCCACATCAACAACGTAACAAAGAACATAGCTGTTATTGGCATTTTATACGAATACGGACCACTCGATCCATTCATCTTCATGTTAGAAAGGGACATACAAAATCTAGATATTGAAGGAGGCTTGGAATTTCTTGGGCAAGTGGATCCTAGAGACGTAAAGCTTGGTGGCAGGAAATACTTTAGATACATGGGATCTCTGACCACTCCTCCGTGTACTGAAGGTGTCACTTGGACTGTCATGGACAAGGTACAAACTGTGGCACCTTATCAAGTCCGGCTTCTTAAACGAGCTTTGGTTGAAGAGAAGAATGCGAGGCCTTTGCAAAAGGTCAACGGTAGAGATGTCTCCTACTTTGACCCATACTCGTGGGTGGGAAGTGTAGCAGCTGAGTAG

Protein sequence

MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEINGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGKMSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSREQVSLLRSAVHDRGMQDQSNHIMDDTSKEMEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGKLQSPISLSKWTADLNQGLDWDGNDAGSIKIKNIDYQLVNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNLDIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLKRALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE
Homology
BLAST of Cp4.1LG03g01570 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.0e-56
Identity = 109/194 (56.19%), Postives = 139/194 (71.65%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           MC  G+MQSPIDL ++RV +V  LG+L R Y PS A LKNRGHDI +K+E  AG+I+ING
Sbjct: 62  MCGKGEMQSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKING 121

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
             Y LQQ HWHS SEHT+NGR + LE+H+VH+  N      +AVV V YKIGR D F+  
Sbjct: 122 FEYELQQLHWHSPSEHTINGRRFALELHMVHEGRN----RRMAVVTVLYKIGRADTFIRS 181

Query: 121 MSRKIKELG--EEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTV 180
           + ++++ +   EE EK  G IDP  IK+G  KYYRY GSLT PPCT+ V W++ +KV+TV
Sbjct: 182 LEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTV 241

Query: 181 SREQVSLLRSAVHD 193
           +R+QV LLR AVHD
Sbjct: 242 TRKQVKLLRVAVHD 251

BLAST of Cp4.1LG03g01570 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 8.7e-52
Identity = 104/210 (49.52%), Postives = 140/210 (66.67%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           +CN G  QSPIDL+++RV ++       R+YKP+ A++ NRGHDI + W+GDAG + I  
Sbjct: 58  VCNTGRYQSPIDLTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRK 117

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
           T++ L Q HWHS SEHT+NG  YDLE+H+VH S    A+   AV+GV YK+G P+ FL K
Sbjct: 118 TDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTS----ARGRTAVIGVLYKLGEPNEFLTK 177

Query: 121 MSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSR 180
           +   IK +G  KE   G IDPR I+    K+YRY+GSLTVPPCTEGVIW + K+V T+S 
Sbjct: 178 LLNGIKAVG-NKEINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISM 237

Query: 181 EQVSLLRSAVHDRGMQDQSNHIMDDTSKEM 211
           EQ++ LR AV D G +  S  + D   + +
Sbjct: 238 EQITALRQAVDD-GFETNSRPVQDSKGRSV 260

BLAST of Cp4.1LG03g01570 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.8e-49
Identity = 100/180 (55.56%), Postives = 127/180 (70.56%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKW-EGDAGSIEIN 60
           MC  G+MQSPIDL +KRV++V  L +L R YKP  A LKNRGHD+ +K+ E  +GSI +N
Sbjct: 61  MCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVN 120

Query: 61  GTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLG 120
           GT Y L Q HWHS SEHT+NGR + LE+H+VH++ N      +AVV V YKIGRPD+FLG
Sbjct: 121 GTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGS----LAVVTVLYKIGRPDSFLG 180

Query: 121 KMSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQT 178
            +  K+  + +  E EK    IDPR IK+G  K+YRY+GSLT PPCT+ VIW + KKV T
Sbjct: 181 LLENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

BLAST of Cp4.1LG03g01570 vs. ExPASy Swiss-Prot
Match: F4HUC4 (Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 8.4e-47
Identity = 100/197 (50.76%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWE-GDAG-SIEI 60
           MC  G+MQSPIDL+ KRV +  +LG L+ +Y PS A +KNRGHDI +K+E G+AG  I I
Sbjct: 57  MCGKGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITI 116

Query: 61  NGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFL 120
           NGT Y LQQ HWHS SEHTLNG+ + LE H+VHQS   D +   AVV  FYK+G+PD FL
Sbjct: 117 NGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSK--DGRN--AVVAFFYKLGKPDYFL 176

Query: 121 GKMSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQ 180
             + R +K + +  E ++    + PR      + YYR++GSLT PPC+E VIW I+K+++
Sbjct: 177 LTLERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMR 236

Query: 181 TVSREQVSLLRSAVHDR 194
           TV+ +Q+ +LR  VHD+
Sbjct: 237 TVTLKQLIMLRVTVHDQ 249

BLAST of Cp4.1LG03g01570 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 1.4e-46
Identity = 96/192 (50.00%), Postives = 133/192 (69.27%), Query Frame = 0

Query: 4   NGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEINGTNY 63
           +G +QSPID+   R +VV +L  L++ YKPS A L NRGHDI ++ + D G ++IN T Y
Sbjct: 58  SGKLQSPIDIFDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLRLD-DGGYLKINETQY 117

Query: 64  VLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGR-PDAFLGKMS 123
            L+Q HWH+ SEHT+NG  ++LE HLVH+S+N        V+G+ Y+IG  PD FL  + 
Sbjct: 118 QLKQLHWHTPSEHTINGERFNLEAHLVHESNNG----KFVVIGIVYEIGLWPDPFLSMIE 177

Query: 124 RKIKELGEEK--EKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSR 183
             +K    +K  E+  G IDP  IK+ G+KY+RY+GSLT PPCTEGV+W I++KV+TV+R
Sbjct: 178 NDLKVPANKKGIERGIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTR 237

Query: 184 EQVSLLRSAVHD 193
            Q+ LL+ AVHD
Sbjct: 238 RQIKLLQEAVHD 244

BLAST of Cp4.1LG03g01570 vs. NCBI nr
Match: KAG7017385.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 790 bits (2040), Expect = 7.29e-287
Identity = 381/396 (96.21%), Postives = 389/396 (98.23%), Query Frame = 0

Query: 66  QQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGKMSRKI 125
           +QAHWHSLSEHTLNGRSY+LEVHLVHQSSNPDAKYPIAVVGVFYKIGRPD FL KMSRKI
Sbjct: 19  KQAHWHSLSEHTLNGRSYELEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDPFLAKMSRKI 78

Query: 126 KELGEEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSREQVSL 185
           KELGE KEK AGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSREQVSL
Sbjct: 79  KELGEAKEKNAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSREQVSL 138

Query: 186 LRSAVHDRGMQDQSNHIMDDTSKEMEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYD 245
           LRSAVHDRGMQDQSNHIMDDTSKEMEA HLL+ AICCLSLLSSGAAAASAAKVGGEFSYD
Sbjct: 139 LRSAVHDRGMQDQSNHIMDDTSKEMEAPHLLMVAICCLSLLSSGAAAASAAKVGGEFSYD 198

Query: 246 PCSDRGPKHWGELDMDWGMCKNGKLQSPISLSKWTADLNQGLDWDGNDAGSIKIKNIDYQ 305
           PCSDRGPKHWGEL+MDWGMCKNGKLQSPISLSKWTADLNQGLDWDGNDAGSIKIKNIDYQ
Sbjct: 199 PCSDRGPKHWGELNMDWGMCKNGKLQSPISLSKWTADLNQGLDWDGNDAGSIKIKNIDYQ 258

Query: 306 LVNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQN 365
           LVNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFI+MLERDIQN
Sbjct: 259 LVNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIYMLERDIQN 318

Query: 366 LDIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLL 425
           LDI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLL
Sbjct: 319 LDIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLL 378

Query: 426 KRALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           KRALVEEKNARPLQK+NGRDVSYFDPYS VGSVAAE
Sbjct: 379 KRALVEEKNARPLQKINGRDVSYFDPYSRVGSVAAE 414

BLAST of Cp4.1LG03g01570 vs. NCBI nr
Match: XP_023527997.1 (alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 516 bits (1330), Expect = 5.02e-181
Identity = 252/275 (91.64%), Postives = 252/275 (91.64%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK
Sbjct: 1   MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTADLNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTADLNQGLGDLRRRHRPANAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE
Sbjct: 241 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 275

BLAST of Cp4.1LG03g01570 vs. NCBI nr
Match: XP_022935053.1 (alpha carbonic anhydrase 7-like [Cucurbita moschata])

HSP 1 Score: 500 bits (1288), Expect = 1.22e-174
Identity = 242/275 (88.00%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEA HLL+ AICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGEL+MDWGMCKNGK
Sbjct: 1   MEAPHLLMVAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELNMDWGMCKNGK 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTA LNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTAHLNQGLGDLRRRHRPANAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFI+MLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIYMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQK+NGR+VSYFDPYSWVGSVAAE
Sbjct: 241 RALVEEKNARPLQKINGRNVSYFDPYSWVGSVAAE 275

BLAST of Cp4.1LG03g01570 vs. NCBI nr
Match: KAG6580627.1 (Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 499 bits (1286), Expect = 2.46e-174
Identity = 243/275 (88.36%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEA HLL+ AICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGEL+MDWGMCKNGK
Sbjct: 1   MEAPHLLMVAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELNMDWGMCKNGK 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTADLNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTADLNQGLGDLRRRHRPANAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFI+MLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIYMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQK+NGRDVSYFDPYS VGSVAAE
Sbjct: 241 RALVEEKNARPLQKINGRDVSYFDPYSRVGSVAAE 275

BLAST of Cp4.1LG03g01570 vs. NCBI nr
Match: XP_022983070.1 (alpha carbonic anhydrase 7-like [Cucurbita maxima])

HSP 1 Score: 483 bits (1242), Expect = 1.07e-167
Identity = 236/275 (85.82%), Postives = 243/275 (88.36%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEA HLL+ AICCLSLLS GAA   AAKVGGEFSYDPCSDRGPKHWGEL+ DWGMCKNG+
Sbjct: 1   MEAPHLLMLAICCLSLLSRGAA---AAKVGGEFSYDPCSDRGPKHWGELNKDWGMCKNGR 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTADLNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTADLNQGLGDLRRRQRPADAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGP DPFI+MLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPPDPFIYMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQK+NGRDVSYFDPYS VGSVAAE
Sbjct: 241 RALVEEKNARPLQKINGRDVSYFDPYSRVGSVAAE 272

BLAST of Cp4.1LG03g01570 vs. ExPASy TrEMBL
Match: A0A6J1F9G4 (alpha carbonic anhydrase 7-like OS=Cucurbita moschata OX=3662 GN=LOC111442037 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 5.91e-175
Identity = 242/275 (88.00%), Postives = 249/275 (90.55%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEA HLL+ AICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGEL+MDWGMCKNGK
Sbjct: 1   MEAPHLLMVAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELNMDWGMCKNGK 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTA LNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTAHLNQGLGDLRRRHRPANAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFI+MLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIYMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQK+NGR+VSYFDPYSWVGSVAAE
Sbjct: 241 RALVEEKNARPLQKINGRNVSYFDPYSWVGSVAAE 275

BLAST of Cp4.1LG03g01570 vs. ExPASy TrEMBL
Match: A0A6J1J4N7 (alpha carbonic anhydrase 7-like OS=Cucurbita maxima OX=3661 GN=LOC111481725 PE=3 SV=1)

HSP 1 Score: 483 bits (1242), Expect = 5.20e-168
Identity = 236/275 (85.82%), Postives = 243/275 (88.36%), Query Frame = 0

Query: 210 MEASHLLIFAICCLSLLSSGAAAASAAKVGGEFSYDPCSDRGPKHWGELDMDWGMCKNGK 269
           MEA HLL+ AICCLSLLS GAA   AAKVGGEFSYDPCSDRGPKHWGEL+ DWGMCKNG+
Sbjct: 1   MEAPHLLMLAICCLSLLSRGAA---AAKVGGEFSYDPCSDRGPKHWGELNKDWGMCKNGR 60

Query: 270 LQSPISLSKWTADLNQGL-----------------------DWDGNDAGSIKIKNIDYQL 329
           LQSPISLSKWTADLNQGL                       DWDGNDAGSIKIKNIDYQL
Sbjct: 61  LQSPISLSKWTADLNQGLGDLRRRQRPADAYLKNDGHEIVVDWDGNDAGSIKIKNIDYQL 120

Query: 330 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL 389
           VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGP DPFI+MLERDIQNL
Sbjct: 121 VNYHWHHPSEHTQDGKTFPLELHMVHINNVTKNIAVIGILYEYGPPDPFIYMLERDIQNL 180

Query: 390 DIEGGLEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 449
           DI+GG+EFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK
Sbjct: 181 DIDGGVEFLGQVDPRDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLK 240

Query: 450 RALVEEKNARPLQKVNGRDVSYFDPYSWVGSVAAE 461
           RALVEEKNARPLQK+NGRDVSYFDPYS VGSVAAE
Sbjct: 241 RALVEEKNARPLQKINGRDVSYFDPYSRVGSVAAE 272

BLAST of Cp4.1LG03g01570 vs. ExPASy TrEMBL
Match: A0A6P6FXR1 (Carbonic anhydrase OS=Ziziphus jujuba OX=326968 GN=LOC107411388 PE=3 SV=1)

HSP 1 Score: 402 bits (1033), Expect = 1.80e-133
Identity = 216/479 (45.09%), Postives = 297/479 (62.00%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           +C+NG MQSPIDL S+RV+VV  LG+LK  YK S A LKNRGHD+ +KWEGDAG++EING
Sbjct: 29  ICSNGSMQSPIDLLSERVEVVSHLGRLKMNYKASNATLKNRGHDMMLKWEGDAGNLEING 88

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
           T Y L+Q HWH+ SEHT+NG+ YD+EVHLVH+S   +    I V+G+ YKIGRPD+FL  
Sbjct: 89  TEYALRQCHWHTPSEHTVNGKKYDVEVHLVHESPQGN----ITVIGILYKIGRPDSFLSS 148

Query: 121 MSRKIKELG--EEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTV 180
           M   ++E+   EE E+  G ++P+ IK+G  KYYRY+GSLT PPCT+ V+W I KKV+TV
Sbjct: 149 MEDILEEISGSEEGERVVGIVNPKNIKIGSRKYYRYIGSLTTPPCTQNVVWTIVKKVRTV 208

Query: 181 SREQVSLLRSAVHDRGMQDQSNHIMDDTSKEMEASHLLIFAICCLSLLSSGAAAASAAKV 240
           +R+Q+ LLR AVHD  ++D+S                                       
Sbjct: 209 TRKQMRLLRVAVHD--VKDES--------------------------------------- 268

Query: 241 GGEFSYDPCSDRGPKHWGELDMDWGMCKNGKLQSPISLSKWTADL--------------- 300
             EFSY   S+ GP  WGE+  +W +C NG +QSPI L     ++               
Sbjct: 269 --EFSYVEGSENGPSRWGEIRPEWSICSNGSMQSPIDLLSERVEVVSHLGRLKMNYKASN 328

Query: 301 ----NQGLD----WDGNDAGSIKIKNIDYQLVNYHWHHPSEHTQDGKTFPLELHMVHINN 360
               N+G D    W+G DAG+++I   +Y L   HWH PSEHT +GK + +E+H+VH  +
Sbjct: 329 ATLKNRGHDMMLKWEG-DAGNLEINGTEYALRQCHWHTPSEHTVNGKKYDVEVHLVH-ES 388

Query: 361 VTKNIAVIGILYEYGPLDPFIFMLERDIQNLD-IEGGLEFLGQVDPRDVKLGGRKYFRYM 420
              NI VIGILY+ G  D F+  +E  ++ +   E G   +G V+P+++K+G RKY+RY+
Sbjct: 389 QQGNITVIGILYKIGRPDSFLSSMEDILEEISGSEEGERVVGIVNPKNIKIGSRKYYRYI 448

Query: 421 GSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLKRALVEEK--NARPLQKVNGRDVSYFDP 451
           GSLTTPPCT+ V WT++ KV+TV   Q+RLL+ A+ ++   NARPLQ +N R V  + P
Sbjct: 449 GSLTTPPCTQNVVWTIVKKVRTVTRKQMRLLRVAVHDDSHTNARPLQAINKRSVLMYRP 458

BLAST of Cp4.1LG03g01570 vs. ExPASy TrEMBL
Match: A0A3P5YBX5 (Carbonic anhydrase OS=Brassica campestris OX=3711 GN=BRAA06T23722Z PE=3 SV=1)

HSP 1 Score: 389 bits (999), Expect = 3.68e-127
Identity = 217/492 (44.11%), Postives = 307/492 (62.40%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           MC  G++QSPIDL ++RVK+V  LG+L R Y+ S A +KNRGHDI +K+E  AGSI+ING
Sbjct: 59  MCGKGEIQSPIDLMNERVKIVSHLGRLIRDYEASNATIKNRGHDIMLKFEAGAGSIKING 118

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
             Y LQQ HWHS SEHT+NGR + LE+H+VH+  N      +AVV V YKIGR D F+  
Sbjct: 119 FQYELQQLHWHSPSEHTVNGRRFALELHMVHEGKNGR----MAVVTVLYKIGRADTFIRS 178

Query: 121 MSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTV 180
           + ++++ + +  + EK    IDP+ IK+G  KYYRY+GSLT PPCT+ V W++ +K++TV
Sbjct: 179 LEKELEAITDLDDAEKHVRMIDPKQIKIGSRKYYRYIGSLTTPPCTQNVTWSVVRKIRTV 238

Query: 181 SREQVSLLRSAVHD------RGMQDQSNHIM----DDTSKEMEASHL--LIFAICCLSLL 240
           +REQV LLR AVHD      R +Q  +  ++      T +EME S +  + F     +L 
Sbjct: 239 TREQVRLLRVAVHDDSLTNARPVQPINKRVVRLYRPRTKQEMETSPIRCIFFVFIIFTLF 298

Query: 241 SSGAAAASAAKVGGE--FSYDPCSDRGPKHWGELDMDWGMCKNGKLQSPISLSKWTADLN 300
           S   AA S  +V  E  F+Y    D+GP+ WGE+  +W MC  G+LQSPI +      + 
Sbjct: 299 SFSTAATSNGEVEDEREFNYKKNDDKGPERWGEIKPEWEMCGKGELQSPIDMMNERVKIV 358

Query: 301 QGL-----DWD---------GND--------AGSIKIKNIDYQLVNYHWHHPSEHTQDGK 360
             L     D++         G+D        AGSIKI    Y+L   HWH PSEHT +G+
Sbjct: 359 SHLGRLIRDYEPSNATIKNRGHDIMLKFEDGAGSIKINGFQYELQQLHWHSPSEHTVNGR 418

Query: 361 TFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL-DIEGGLEFLGQVDPR 420
            F LELHMVH       +AV+ +LY+ G  D FI  LE++++ + D++   + +  +DP+
Sbjct: 419 RFALELHMVH-EGKNGRMAVVTVLYKIGRADTFIRSLEKELEAITDLDDAEKHVRMIDPK 478

Query: 421 DVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLKRALVEEK--NARPLQ 451
            +K+G RKY+RY+GSLTTPPCT+ VTW+V+ K++TV   QVRLL+ A+ ++   NARP+Q
Sbjct: 479 QIKIGSRKYYRYIGSLTTPPCTQNVTWSVVRKIRTVTREQVRLLRVAVHDDSLTNARPVQ 538

BLAST of Cp4.1LG03g01570 vs. ExPASy TrEMBL
Match: A0A3P6F3I2 (Carbonic anhydrase OS=Brassica oleracea OX=3712 GN=BOLC5T29371H PE=3 SV=1)

HSP 1 Score: 387 bits (995), Expect = 1.52e-126
Identity = 219/493 (44.42%), Postives = 309/493 (62.68%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWE-GDAGSIEIN 60
           MC  G++QSPIDL ++RVK+V  LG+L R Y+PS A +KNRGHDI +K+E G AGSI+IN
Sbjct: 59  MCGKGEIQSPIDLMNERVKIVSHLGRLIRDYEPSNATIKNRGHDIMLKFEDGAAGSIKIN 118

Query: 61  GTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLG 120
           G  Y LQQ HWHS SEHT+NGR + LE+H+VH+      K  +AVV V YKIGR D F+ 
Sbjct: 119 GFQYELQQLHWHSPSEHTINGRRFALELHMVHEGK----KGRMAVVTVLYKIGRADTFIR 178

Query: 121 KMSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQT 180
            + ++++ + +  + EK    IDP+ IK+G  KYYRY+GSLT PPCT+ V W++ +K++T
Sbjct: 179 SLEKELEAITDLDDAEKHVRMIDPKQIKIGSRKYYRYIGSLTTPPCTQNVTWSVVRKIRT 238

Query: 181 VSREQVSLLRSAVHD------RGMQDQSNHIMD----DTSKEMEASHL--LIFAICCLSL 240
           V+REQV LLR AVHD      R +Q  +  ++      T +EME+S +  + F     +L
Sbjct: 239 VTREQVRLLRVAVHDDSLTNARPVQPINKRVVHLYRPRTKQEMESSPIQCIFFVFIIFTL 298

Query: 241 LSSGAAAASAAKVGGE--FSYDPCSDRGPKHWGELDMDWGMCKNGKLQSPISLSKWTADL 300
            S   AA S  +V  E  F+Y    ++GP+ WGE+  +W MC  G+LQSPI L      +
Sbjct: 299 FSFSTAATSHGEVEDEHEFNYKKNDNKGPERWGEIKPEWEMCGKGELQSPIDLMNERVKI 358

Query: 301 NQGL-----DWD---------GND--------AGSIKIKNIDYQLVNYHWHHPSEHTQDG 360
              L     D++         G+D        AGSIKI    Y+L   HWH PSEHT +G
Sbjct: 359 VSHLGRLIRDYEPSNATIKNRGHDIMLKFKDGAGSIKINGFHYELQQLHWHSPSEHTVNG 418

Query: 361 KTFPLELHMVHINNVTKNIAVIGILYEYGPLDPFIFMLERDIQNL-DIEGGLEFLGQVDP 420
           + F LELHMVH       +AV+ +LY+ G  D FI  LE+++  + D++   + +  +DP
Sbjct: 419 RRFALELHMVH-EGKNGRMAVVTVLYKIGRADTFIRSLEKELDAITDLDDAEKHVRMIDP 478

Query: 421 RDVKLGGRKYFRYMGSLTTPPCTEGVTWTVMDKVQTVAPYQVRLLKRALVEEK--NARPL 451
           + +K+G RKY+RY+GSLTTPPCT+ VTW+V+ K++TV   QVRLL+ A+ ++   NARP+
Sbjct: 479 KQIKIGSRKYYRYIGSLTTPPCTQNVTWSVVRKIRTVTREQVRLLRVAVHDDSLTNARPV 538

BLAST of Cp4.1LG03g01570 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-57
Identity = 109/194 (56.19%), Postives = 139/194 (71.65%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           MC  G+MQSPIDL ++RV +V  LG+L R Y PS A LKNRGHDI +K+E  AG+I+ING
Sbjct: 62  MCGKGEMQSPIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKING 121

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
             Y LQQ HWHS SEHT+NGR + LE+H+VH+  N      +AVV V YKIGR D F+  
Sbjct: 122 FEYELQQLHWHSPSEHTINGRRFALELHMVHEGRN----RRMAVVTVLYKIGRADTFIRS 181

Query: 121 MSRKIKELG--EEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTV 180
           + ++++ +   EE EK  G IDP  IK+G  KYYRY GSLT PPCT+ V W++ +KV+TV
Sbjct: 182 LEKELEGIAEMEEAEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTV 241

Query: 181 SREQVSLLRSAVHD 193
           +R+QV LLR AVHD
Sbjct: 242 TRKQVKLLRVAVHD 251

BLAST of Cp4.1LG03g01570 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 206.1 bits (523), Expect = 6.2e-53
Identity = 104/210 (49.52%), Postives = 140/210 (66.67%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           +CN G  QSPIDL+++RV ++       R+YKP+ A++ NRGHDI + W+GDAG + I  
Sbjct: 58  VCNTGRYQSPIDLTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRK 117

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
           T++ L Q HWHS SEHT+NG  YDLE+H+VH S    A+   AV+GV YK+G P+ FL K
Sbjct: 118 TDFNLVQCHWHSPSEHTVNGTRYDLELHMVHTS----ARGRTAVIGVLYKLGEPNEFLTK 177

Query: 121 MSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQTVSR 180
           +   IK +G  KE   G IDPR I+    K+YRY+GSLTVPPCTEGVIW + K+V T+S 
Sbjct: 178 LLNGIKAVG-NKEINLGMIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISM 237

Query: 181 EQVSLLRSAVHDRGMQDQSNHIMDDTSKEM 211
           EQ++ LR AV D G +  S  + D   + +
Sbjct: 238 EQITALRQAVDD-GFETNSRPVQDSKGRSV 260

BLAST of Cp4.1LG03g01570 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-50
Identity = 100/180 (55.56%), Postives = 127/180 (70.56%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKW-EGDAGSIEIN 60
           MC  G+MQSPIDL +KRV++V  L +L R YKP  A LKNRGHD+ +K+ E  +GSI +N
Sbjct: 33  MCGKGEMQSPIDLMNKRVRLVTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVN 92

Query: 61  GTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLG 120
           GT Y L Q HWHS SEHT+NGR + LE+H+VH++ N      +AVV V YKIGRPD+FLG
Sbjct: 93  GTEYKLLQLHWHSPSEHTMNGRRFALELHMVHENINGS----LAVVTVLYKIGRPDSFLG 152

Query: 121 KMSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQT 178
            +  K+  + +  E EK    IDPR IK+G  K+YRY+GSLT PPCT+ VIW + KKV T
Sbjct: 153 LLENKLSAITDQNEAEKYVDVIDPRDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 208

BLAST of Cp4.1LG03g01570 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 189.5 bits (480), Expect = 6.0e-48
Identity = 100/197 (50.76%), Postives = 136/197 (69.04%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWE-GDAG-SIEI 60
           MC  G+MQSPIDL+ KRV +  +LG L+ +Y PS A +KNRGHDI +K+E G+AG  I I
Sbjct: 57  MCGKGNMQSPIDLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITI 116

Query: 61  NGTNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFL 120
           NGT Y LQQ HWHS SEHTLNG+ + LE H+VHQS   D +   AVV  FYK+G+PD FL
Sbjct: 117 NGTEYKLQQIHWHSPSEHTLNGKRFVLEEHMVHQSK--DGRN--AVVAFFYKLGKPDYFL 176

Query: 121 GKMSRKIKELGE--EKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKVQ 180
             + R +K + +  E ++    + PR      + YYR++GSLT PPC+E VIW I+K+++
Sbjct: 177 LTLERYLKRITDTHESQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMR 236

Query: 181 TVSREQVSLLRSAVHDR 194
           TV+ +Q+ +LR  VHD+
Sbjct: 237 TVTLKQLIMLRVTVHDQ 249

BLAST of Cp4.1LG03g01570 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 186.4 bits (472), Expect = 5.1e-47
Identity = 89/175 (50.86%), Postives = 121/175 (69.14%), Query Frame = 0

Query: 1   MCNNGDMQSPIDLSSKRVKVVKSLGQLKRKYKPSMAILKNRGHDITIKWEGDAGSIEING 60
           +C+ G +QSPIDL+ +RV ++     L + YKP+ A++++RGHD+ + W+GD G I I+ 
Sbjct: 59  VCSTGKIQSPIDLTDERVSLIHDQA-LSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQ 118

Query: 61  TNYVLQQAHWHSLSEHTLNGRSYDLEVHLVHQSSNPDAKYPIAVVGVFYKIGRPDAFLGK 120
           T+Y L Q HWHS SEHT+NG SYDLE+H+VH S    A     VVGV YK+G PD FL K
Sbjct: 119 TDYKLVQCHWHSPSEHTINGTSYDLELHMVHTS----ASGKTTVVGVLYKLGEPDEFLTK 178

Query: 121 MSRKIKELGEEKEKKAGRIDPRGIKMGGEKYYRYMGSLTVPPCTEGVIWNINKKV 176
           +   IK +G +KE   G +DPR I+     +YRY+GSLT+PPCTEGVIW + K+V
Sbjct: 179 ILNGIKGVG-KKEIDLGIVDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTVQKRV 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8172.0e-5656.19Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1[more]
F4JIK28.7e-5249.52Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1[more]
F4IHR41.8e-4955.56Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2[more]
F4HUC48.4e-4750.76Alpha carbonic anhydrase 5 OS=Arabidopsis thaliana OX=3702 GN=ACA5 PE=3 SV=1[more]
Q84UV81.4e-4650.00Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS... [more]
Match NameE-valueIdentityDescription
KAG7017385.17.29e-28796.21Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023527997.15.02e-18191.64alpha carbonic anhydrase 7-like [Cucurbita pepo subsp. pepo][more]
XP_022935053.11.22e-17488.00alpha carbonic anhydrase 7-like [Cucurbita moschata][more]
KAG6580627.12.46e-17488.36Alpha carbonic anhydrase 7, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022983070.11.07e-16785.82alpha carbonic anhydrase 7-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1F9G45.91e-17588.00alpha carbonic anhydrase 7-like OS=Cucurbita moschata OX=3662 GN=LOC111442037 PE... [more]
A0A6J1J4N75.20e-16885.82alpha carbonic anhydrase 7-like OS=Cucurbita maxima OX=3661 GN=LOC111481725 PE=3... [more]
A0A6P6FXR11.80e-13345.09Carbonic anhydrase OS=Ziziphus jujuba OX=326968 GN=LOC107411388 PE=3 SV=1[more]
A0A3P5YBX53.68e-12744.11Carbonic anhydrase OS=Brassica campestris OX=3711 GN=BRAA06T23722Z PE=3 SV=1[more]
A0A3P6F3I21.52e-12644.42Carbonic anhydrase OS=Brassica oleracea OX=3712 GN=BOLC5T29371H PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08080.11.4e-5756.19alpha carbonic anhydrase 7 [more]
AT4G20990.16.2e-5349.52alpha carbonic anhydrase 4 [more]
AT2G28210.11.3e-5055.56alpha carbonic anhydrase 2 [more]
AT1G08065.16.0e-4850.76alpha carbonic anhydrase 5 [more]
AT4G21000.15.1e-4750.86alpha carbonic anhydrase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001148Alpha carbonic anhydrase domainSMARTSM01057Carb_anhydrase_2acoord: 1..204
e-value: 3.2E-44
score: 162.9
coord: 242..448
e-value: 2.2E-44
score: 163.5
IPR001148Alpha carbonic anhydrase domainPFAMPF00194Carb_anhydrasecoord: 2..204
e-value: 5.3E-36
score: 124.5
coord: 304..448
e-value: 3.3E-25
score: 89.1
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 240..450
score: 26.930769
IPR001148Alpha carbonic anhydrase domainPROSITEPS51144ALPHA_CA_2coord: 1..214
score: 37.316742
IPR036398Alpha carbonic anhydrase domain superfamilyGENE3D3.10.200.10Alpha carbonic anhydrasecoord: 239..454
e-value: 2.2E-54
score: 186.5
coord: 1..212
e-value: 2.1E-58
score: 199.6
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 1..193
IPR036398Alpha carbonic anhydrase domain superfamilySUPERFAMILY51069Carbonic anhydrasecoord: 241..446
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 288..451
coord: 2..195
IPR023561Carbonic anhydrase, alpha-classPANTHERPTHR18952CARBONIC ANHYDRASEcoord: 215..283
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 288..451
coord: 215..283
NoneNo IPR availablePANTHERPTHR18952:SF201ALPHA CARBONIC ANHYDRASE 3coord: 2..195
IPR018338Carbonic anhydrase, alpha-class, conserved sitePROSITEPS00162ALPHA_CA_1coord: 74..90
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 251..446
e-value: 5.00765E-75
score: 232.547
IPR041891Carbonic anhydrase, prokaryotic-likeCDDcd03124alpha_CA_prokaryotic_likecoord: 1..191
e-value: 1.74696E-73
score: 228.695

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g01570.1Cp4.1LG03g01570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0004089 carbonate dehydratase activity
molecular_function GO:0008270 zinc ion binding