Cp4.1LG02g16410 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g16410 vs. ExPASy Swiss-Prot
Match: O22243 (DExH-box ATP-dependent RNA helicase DExH8 OS=Arabidopsis thaliana OX=3702 GN=At2g47680 PE=2 SV=2)

HSP 1 Score: 931.4 bits (2406), Expect = 7.6e-270
Identity = 508/1007 (50.45%), Postives = 663/1007 (65.84%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSS  L     +F++LP+M++K RI++KI +NR+TLIVGE GCGKSSQ+PQFLL+ +
Sbjct: 8    SSSSSSESLPLPSSNFASLPIMAMKRRIIDKILENRVTLIVGEPGCGKSSQVPQFLLEAN 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            M PILCTQPRRFAVVA+A MVA++RN ++G E+GYHIGHSK  +E SKI+FKTAGVLL+E
Sbjct: 68   MAPILCTQPRRFAVVAVAKMVAKSRNSDLGGEIGYHIGHSKILTEGSKILFKTAGVLLDE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            M D+GL AL YKVI+LDEVHERSVESDLVLVCVKQFL+KN+DLRVVLMSATADI RYRDY
Sbjct: 128  MLDKGLNALKYKVIILDEVHERSVESDLVLVCVKQFLMKNNDLRVVLMSATADITRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQS-SNGFSPSTSSVEI 241
            FKE+GRGERVEV+AIPS  Q+T F+R+V YLE+V  LLG+ SDL +   G SPS++  EI
Sbjct: 188  FKELGRGERVEVVAIPSPDQRTIFQRRVLYLEQVAGLLGVSSDLSAYCPGPSPSSADTEI 247

Query: 242  KPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKS-LSSFKVYILHSSID 301
            KPE+  LIH+L+L+IH+ E DIEKSIL+FLPTYYSLEQQ+H L+   +SF+V+ILH SID
Sbjct: 248  KPELQNLIHDLILYIHEKEPDIEKSILVFLPTYYSLEQQYHQLEPFFASFEVHILHRSID 307

Query: 302  IEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVW 361
             EQAL AM+I +S RKVILATNIAESSVTIPKVAYVIDSCRSLQVFWD ++K+D+ Q+VW
Sbjct: 308  TEQALAAMKICRSRRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDPSRKRDAVQLVW 367

Query: 362  ISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKA 421
            +S+SQAEQRRGRTGRTCDG+VYRLVP +F++K E+ E P+IL+LSLRQQVL IC TES+A
Sbjct: 368  VSRSQAEQRRGRTGRTCDGEVYRLVPSAFFNKLEEHEPPSILKLSLRQQVLHICCTESRA 427

Query: 422  INDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSS 481
            IND   LL KA+DPPD +VV+DAL +L+ ++AL+K+PRGRYEPT+YG LLASF LSFD+S
Sbjct: 428  INDANALLAKAMDPPDPDVVDDALRMLLSIQALRKSPRGRYEPTFYGRLLASFPLSFDAS 487

Query: 482  VLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL---------------------- 541
            +L++KFG++GML +GILLG+LMDT PLP+  PFG+++L                      
Sbjct: 488  ILVVKFGEMGMLRQGILLGVLMDTLPLPIHHPFGDDSLFLEYVDHYFGGSKTISGGRREM 547

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DK R+E L +L+  ++ +       + E+EWC FHN       
Sbjct: 548  VLMANFCAFQFWQRVFKDKHRLENLKQLLSKEKDKDLKLMFPEIEKEWCDFHNIAQSSFY 607

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTP-QFQHSCILKC--------HENGVDQS 661
                  ED + + H+FRP+F+   D   +   P +F H+C ++C        H   VD +
Sbjct: 608  HVSELYEDTLSSFHRFRPQFISSSDSQPTYYNPYEFDHTCYIECQPSEDKYLHSEDVDNN 667

Query: 662  S---ESRTCASLPYVASSYPRTNHVAGKLADVIKQMK----------------------- 721
                E R C S+P+V  +  + N +A  +A +IK+++                       
Sbjct: 668  QPPPEVRKCVSVPFVPPNAFQANAIAENMASIIKEIRTQCTPSESDNGHGALEPEDYVEY 727

Query: 722  ----------------------------------------GCRNGDSCFFSHDQSPSNSP 781
                                                    GCRNG+SC FSH      + 
Sbjct: 728  GEAPVCVYFLNGYCNRGGQCTFTHTLQSTRPACKFFASSQGCRNGESCLFSHAMRRRTTS 787

Query: 782  SFESTLCLPEDEKAHALTLEKYFPKSG-GYILVMDDAGFHFSSNLARHCDPSKIICTTNI 841
                  CLPE++ +    L   FP S  G ILV DD+  HF+S++A      +I+ T++ 
Sbjct: 788  YLPPPQCLPEEDGSSTSPLLDLFPTSSEGCILVFDDSDMHFTSSIANRYPSWRILSTSSS 847

Query: 842  SRSDVYDAALKDARKFWELSHPDETIISSG--KNQIPWFDVKCILWFPRFASSKENLVIE 863
            S +   D++L D R FW L+HP +TIIS    +N IPW +VKC+LWF    S  +    +
Sbjct: 848  SETLFCDSSLADTRIFWGLNHPYQTIISKAGRENPIPWNEVKCVLWFLNPDSYADTPEKQ 907

BLAST of Cp4.1LG02g16410 vs. ExPASy Swiss-Prot
Match: Q1EHT7 (Zinc finger CCCH domain-containing protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0256800 PE=2 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 3.9e-234
Identity = 461/992 (46.47%), Postives = 617/992 (62.20%), Query Frame = 0

Query: 20   LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDEDMGPILCTQPRRFAVVAIA 79
            L V +L+ +IVEK++ NR+TLIVG+TGCGKSS +PQFLL+E+M PILCTQPRRFAVVAIA
Sbjct: 18   LAVEALRGKIVEKVKGNRVTLIVGDTGCGKSSMVPQFLLEENMEPILCTQPRRFAVVAIA 77

Query: 80   NMVARARNCNVGEEVGYHIGHSKHS---SERSKIVFKTAGVLLEEMRDRGLKALDYKVIV 139
             M+A +RNC VGEEVGYHIGHS  S   S+RS+IVFKTAGV+LE+MRD+G+ AL+YKVI+
Sbjct: 78   QMIAESRNCQVGEEVGYHIGHSNVSNLNSKRSRIVFKTAGVVLEQMRDKGIAALNYKVII 137

Query: 140  LDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYFKEIGRGERVEVLAI 199
            LDE+HERSVESDLVL CVKQF++K +DLR++LMSATADI RY+DYF+++GRGERVEV+AI
Sbjct: 138  LDEIHERSVESDLVLACVKQFMMKKNDLRLILMSATADITRYKDYFRDLGRGERVEVIAI 197

Query: 200  PSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFS-PSTSS-VEIKPEVHKLIHNLLLH 259
            PSS + + F+RKV YLE++ ++L ++S+  S+   S P+T++   +KP+V++LIH LLLH
Sbjct: 198  PSSPRSSIFQRKVLYLEQIVDILKMDSESLSTKYCSGPNTAADAGLKPDVYELIHRLLLH 257

Query: 260  IHKNESDIEKSILIFLPTYYSLEQQW-HLLKSLSSFKVYILHSSIDIEQALTAMRIWKSH 319
            IH+NE DI KSIL+FLPTYY+LEQQW  LL + S FKV+ILH SID ++AL  M++ KS 
Sbjct: 258  IHQNEPDIGKSILVFLPTYYALEQQWIRLLSASSMFKVHILHRSIDTDEALQTMKVSKSC 317

Query: 320  RKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTG 379
            RKVILATNIAESSVTIP VAYVIDSCRSLQV+WD  +K DSA++VW+SKSQAEQR+GRTG
Sbjct: 318  RKVILATNIAESSVTIPGVAYVIDSCRSLQVYWDPIRKTDSAELVWVSKSQAEQRKGRTG 377

Query: 380  RTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDP 439
            RTCDGQ+YRLV   FY+   D E PAILRLSLR+QVL+IC  ES+A+NDP VLLQK LDP
Sbjct: 378  RTCDGQIYRLVTGPFYNSLTDHEYPAILRLSLREQVLMICCAESRAMNDPHVLLQKVLDP 437

Query: 440  PDGNVVEDALNLLVRMKALKK--TPRGRYEPTYYGSLLASFSLSFDSSVLILKFGDIGML 499
            PD +VVEDAL  LV+++AL K  +PRGR+EPT+YG LL S  LSFD+SVL LKFGD G +
Sbjct: 438  PDSDVVEDALESLVQIRALDKPTSPRGRHEPTFYGCLLNSLPLSFDASVLALKFGDTGSI 497

Query: 500  HEGILLGILMDTQPLPVLRPFGENNL---------------------------------- 559
             EGIL+ I++D QPLP+++PFG   L                                  
Sbjct: 498  CEGILISIMLDIQPLPIVQPFGHQQLCKMYRNNYFEEEGIDLQTGKKEAALVGNLCAFQF 557

Query: 560  ------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN-------------EDIIH 619
                  DK R++ L  +V + E +A+    +K E+EWC+FHN             +DI+ 
Sbjct: 558  WQRMFKDKYRLDCLINVVNTHEPKASNGFVAKPEDEWCAFHNLVPTALNYISEIYDDIMG 617

Query: 620  TLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHE-------NGVDQSSES------RTCA 679
            TLH+FRP FL   +        +F H C+   HE       N +   +E+      R CA
Sbjct: 618  TLHRFRPSFLVKINPPMYLQPSEFHHMCLR--HEVLELENVNSLPLEAENSHLDSHRRCA 677

Query: 680  SLPYVASSYPRTNHVAGKLADVIKQMK--------------------------------- 739
            + PYV+ +   T  V   L  +IK+MK                                 
Sbjct: 678  ATPYVSPADFGTTTVVKTLKTLIKEMKTQSAADRVTYRELVHGYVQPALENEMCVFFLNG 737

Query: 740  ----------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDE 799
                                        GCRNG+SC FSHD     S S  S +C  ++ 
Sbjct: 738  SCNRGDTCHFSHSSRAPRPICKFFLTLQGCRNGNSCSFSHDSGSLVSSSITSGIC-SQEN 797

Query: 800  KAHALTLEKYFPKSG-GYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKD 859
            +A ++  ++  P +G G+ILVM+D    F+  L  + DP+KII  T    S   D+  K 
Sbjct: 798  RATSVCCKRLLPAAGDGHILVMNDKSLQFACKLCNYYDPTKIIACTPGPHSFESDSVTKG 857

Query: 860  ARKFWELSHPDETII-SSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAV 861
             +    L+ P    I    K  +PW  +  + WF    S++   + E+++LQ FF  +A+
Sbjct: 858  LKILQNLADPSYLFIGGEHKLSVPWTKLSRVFWFADIDSNES--ISEQVVLQKFFQHIAI 917

BLAST of Cp4.1LG02g16410 vs. ExPASy Swiss-Prot
Match: Q6PGC1 (ATP-dependent RNA helicase DHX29 OS=Mus musculus OX=10090 GN=Dhx29 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.5e-60
Identity = 181/558 (32.44%), Postives = 282/558 (50.54%), Query Frame = 0

Query: 20   LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLD----EDMGP----ILCTQPR 79
            LPV   ++ IVE ++++R+ ++ GETG GKS+Q+P FLL+    ++ G     I+CTQPR
Sbjct: 571  LPVFKHRDSIVETLKRHRVVVVAGETGSGKSTQVPHFLLEDLLLDECGARKCNIVCTQPR 630

Query: 80   RFAVVAIANMVARARNCNVG-----EEVGYHIGHSKHSSERSKIVFKTAGVLLEEMRDRG 139
            R + V++A  V     C  G        GY I     +SE +++++ T GVLL ++++ G
Sbjct: 631  RISAVSLATRVCEELGCESGPGGRNSLCGYQIRMESRASESTRLLYCTTGVLLRKLQEDG 690

Query: 140  LKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYFKEI- 199
            L A D   +++DEVHERSV+SD +LV +K+ L K  DL ++LMSAT D  ++  YF    
Sbjct: 691  LLA-DVSHVIVDEVHERSVQSDFLLVILKEILQKRSDLHLILMSATVDSDKFSTYFTHCP 750

Query: 200  -----GRGERVEVLAIPSSSQKTFF------ERKVSYLEEVNEL-LGIESDLQSSNGFS- 259
                 GR   VEV  +    ++T F      E    +LEE  E+ + + S       +  
Sbjct: 751  ILRISGRSYPVEVFHLEDIVEETGFVLEKDSEYCQKFLEEEEEITINVTSKAGGVKKYQE 810

Query: 260  ----PSTSSVEIKPEVHK--------------------LIHNLLLHIHKNES--DIEKSI 319
                 S +S E+ P   K                    LI  LL+++ K+    +IE ++
Sbjct: 811  YIPVQSGASPELNPFYQKYSSRTQHAILYMNPHKINLDLILELLVYLDKSPQFRNIEGAV 870

Query: 320  LIFLPTYYSLEQQWHLLKS-----LSSFKVYILHSSIDI-EQALTAMRIWKSHRKVILAT 379
            LIFLP    ++Q + LL S        ++V  LHS +   +QA   M      RK++LAT
Sbjct: 871  LIFLPGLAHIQQLYDLLSSDRRFYSERYQVIALHSVLSTQDQAAAFMFPPPGVRKIVLAT 930

Query: 380  NIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQV 439
            NIAE+ +TIP V +VID+ R+ +  +  + +  S    ++SK+ A QR+GR GR  DG  
Sbjct: 931  NIAETGITIPDVVFVIDTGRTKENKYHESSQMSSLVETFVSKASALQRQGRAGRVRDGFC 990

Query: 440  YRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDPPDGNVVE 499
            +RL  R  +  F D+  P ILR+ L +  L I   +   +  P   L KALDPP   V+ 
Sbjct: 991  FRLYTRERFEGFLDYSVPEILRVPLEELCLHIMKCD---LGSPEDFLSKALDPPQLQVIS 1050

Query: 500  DALNLLVRMKALKKTPRGRYEP--TYYGSLLASFSLSFDSSVLILKFGDIGMLHEGILLG 517
            +A+NLL ++ A +       EP  T  G  LA+  ++     +++     G L     L 
Sbjct: 1051 NAMNLLRKIGACEPN-----EPKLTPLGQHLAALPVNVKIGKMLIFGAIFGCLEPVATLA 1110

BLAST of Cp4.1LG02g16410 vs. ExPASy Swiss-Prot
Match: Q16JS8 (Probable ATP-dependent RNA helicase spindle-E OS=Aedes aegypti OX=7159 GN=spn-E PE=3 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 5.8e-60
Identity = 164/510 (32.16%), Postives = 272/510 (53.33%), Query Frame = 0

Query: 20  LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE-----DMGPILCTQPRRFA 79
           LP+   K+ I+ +IR+N + ++ G TGCGK++Q+PQF+L+E     +   I+ TQPR+ A
Sbjct: 36  LPIRHSKDDILARIRENPVIVLEGPTGCGKTTQVPQFILEEAYHRKEYCNIIVTQPRKIA 95

Query: 80  VVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEEMRDRGLKALDYKV 139
            ++IA  V+  R C +G  VG+ +G  +  S  +++++ T GVLL+ + +    A +Y  
Sbjct: 96  AMSIAKRVSEERKCELGTLVGFKVGLKECLSPDTRLLYCTTGVLLQSLINSKTMA-NYTH 155

Query: 140 IVLDEVHERSVESDLVLVCVKQFLLKNHD-LRVVLMSATADIGRYRDYFKEIGRGERVEV 199
           ++LDE+HER V+ D +L+ V++FL  N    +V+LMSAT D   + +YFK     ++V  
Sbjct: 156 VILDEIHEREVDMDFLLIVVRRFLATNSSKTKVILMSATIDSKAFAEYFK---TPKKVGY 215

Query: 200 LAIP--SSSQKTFFERKVSYLEEVNEL-LGIESDLQSSNGFSPSTSSVEIKPEVHKLIHN 259
           L  P  S  +   +E K  Y +++++L L    D ++  G S    +V  K  +  +   
Sbjct: 216 LTAPIISVDRPRLYEVKEFYYDDLDKLRLDFAIDYENP-GISSHMYTVAAK--LVLVCDR 275

Query: 260 LLLHIHKNE-SDIEKSILIFLPTYYSLEQQWHLL----------KSLSSFKVYILHSSID 319
           L+ ++H  E  + + ++LIFLP    +++  H+L          K   +  ++ LHS + 
Sbjct: 276 LIENMHGEERMEYKPTVLIFLPGINEIDRMDHVLRETLTRIVNPKEKPNLDIHRLHSILP 335

Query: 320 IEQALTAMR-IWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVV 379
            ++ +   R      RKVIL+TNIAESS+T+P V ++ID C    +F D      + +  
Sbjct: 336 ADEQVKVFRKPAPGQRKVILSTNIAESSITVPDVKFIIDFCLQRVLFTDTTTNFSTLRTE 395

Query: 380 WISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFE-RPAILRLSLRQQVLLICSTES 439
           W S++   QR+GR GR  DG+VYRLV R FY     F   P ILR  L   +L     + 
Sbjct: 396 WASQANCIQRQGRAGRVMDGRVYRLVDRRFYENQMRFSTSPEILRCPLENVIL---KAKL 455

Query: 440 KAINDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEP-----TYYGSLLASF 499
             +  P  +L  A++PPD + + + +  L  + AL +T +G YE      TY G ++A  
Sbjct: 456 LEMGPPHSILALAMNPPDLSDIRNTVLQLKELGALVQTVKGNYEQLDGDLTYMGRIMAKL 515

Query: 500 SLSFDSSVLILKFGDIGMLHEGILLGILMD 503
            L    S LI+      +L E I++   M+
Sbjct: 516 PLDLRISKLIILGYIFSVLEESIIIAAGMN 535

BLAST of Cp4.1LG02g16410 vs. ExPASy Swiss-Prot
Match: Q7Z478 (ATP-dependent RNA helicase DHX29 OS=Homo sapiens OX=9606 GN=DHX29 PE=1 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 4.1e-58
Identity = 175/561 (31.19%), Postives = 276/561 (49.20%), Query Frame = 0

Query: 20   LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE--------DMGPILCTQPR 79
            LPV   ++ IVE ++++R+ ++ GETG GKS+Q+P FLL++            I+CTQPR
Sbjct: 572  LPVFKHRDSIVETLKRHRVVVVAGETGSGKSTQVPHFLLEDLLLNEWEASKCNIVCTQPR 631

Query: 80   RFAVVAIANMVARARNCNVG-----EEVGYHIGHSKHSSERSKIVFKTAGVLLEEMRDRG 139
            R + V++AN V     C  G        GY I     + E +++++ T GVLL ++++ G
Sbjct: 632  RISAVSLANRVCDELGCENGPGGRNSLCGYQIRMESRACESTRLLYCTTGVLLRKLQEDG 691

Query: 140  LKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYFKEI- 199
            L + +   +++DEVHERSV+SD +L+ +K+ L K  DL ++LMSAT D  ++  YF    
Sbjct: 692  LLS-NVSHVIVDEVHERSVQSDFLLIILKEILQKRSDLHLILMSATVDSEKFSTYFTHCP 751

Query: 200  -----GRGERVEVLAIPSSSQKTFF------ERKVSYLEEVNEL---------------- 259
                 GR   VEV  +    ++T F      E    +LEE  E+                
Sbjct: 752  ILRISGRSYPVEVFHLEDIIEETGFVLEKDSEYCQKFLEEEEEVTINVTSKAGGIKKYQE 811

Query: 260  -----LGIESDLQS-SNGFSPSTSSVEIKPEVHK----LIHNLLLHIHKNES--DIEKSI 319
                  G  +DL      +S  T    +    HK    LI  LL ++ K+    +IE ++
Sbjct: 812  YIPVQTGAHADLNPFYQKYSSRTQHAILYMNPHKINLDLILELLAYLDKSPQFRNIEGAV 871

Query: 320  LIFLPTYYSLEQQWHLLKS-----LSSFKVYILHSSIDIEQALTAMRI-WKSHRKVILAT 379
            LIFLP    ++Q + LL +        +KV  LHS +  +    A  +     RK++LAT
Sbjct: 872  LIFLPGLAHIQQLYDLLSNDRRFYSERYKVIALHSILSTQDQAAAFTLPPPGVRKIVLAT 931

Query: 380  NIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQV 439
            NIAE+ +TIP V +VID+ R+ +  +  + +  S    ++SK+ A QR+GR GR  DG  
Sbjct: 932  NIAETGITIPDVVFVIDTGRTKENKYHESSQMSSLVETFVSKASALQRQGRAGRVRDGFC 991

Query: 440  YRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDPPDGNVVE 499
            +R+  R  +  F D+  P ILR+ L +  L I       +  P   L KALDPP   V+ 
Sbjct: 992  FRMYTRERFEGFMDYSVPEILRVPLEELCLHIMKCN---LGSPEDFLSKALDPPQLQVIS 1051

Query: 500  DALNLLVRMKAL-----KKTPRGRYEPTYYGSLLASFSLSFDSSVLILKFGDIGMLHEGI 517
            +A+NLL ++ A      K TP G++        LA+  ++     +++     G L    
Sbjct: 1052 NAMNLLRKIGACELNEPKLTPLGQH--------LAALPVNVKIGKMLIFGAIFGCLDPVA 1111

BLAST of Cp4.1LG02g16410 vs. NCBI nr
Match: XP_023525192.1 (DExH-box ATP-dependent RNA helicase DExH8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1663 bits (4306), Expect = 0.0
Identity = 873/1005 (86.87%), Postives = 875/1005 (87.06%), Query Frame = 0

Query: 1    MASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE 60
            MASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE
Sbjct: 1    MASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE 60

Query: 61   DMGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLE 120
            DMGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLE
Sbjct: 61   DMGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLE 120

Query: 121  EMRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRD 180
            EMRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRD
Sbjct: 121  EMRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRD 180

Query: 181  YFKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEI 240
            YFKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEI
Sbjct: 181  YFKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEI 240

Query: 241  KPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDI 300
            KPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDI
Sbjct: 241  KPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDI 300

Query: 301  EQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWI 360
            EQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWI
Sbjct: 301  EQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWI 360

Query: 361  SKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAI 420
            SKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAI
Sbjct: 361  SKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAI 420

Query: 421  NDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSV 480
            NDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSV
Sbjct: 421  NDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSV 480

Query: 481  LILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL----------------------- 540
            LILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                       
Sbjct: 481  LILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKE 540

Query: 541  ------------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------ 600
                              DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFH+      
Sbjct: 541  MALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHSLVHSSL 600

Query: 601  -------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCAS 660
                   EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCAS
Sbjct: 601  NHVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCAS 660

Query: 661  LPYVASSYPRTNHVAGKLADVIKQMK---------------------------------- 720
            LPYVASSYPRTNHVAGKLADVIKQMK                                  
Sbjct: 661  LPYVASSYPRTNHVAGKLADVIKQMKVIYGEEPNNQSLSSLNNDFDVHDGTALCVYFVNG 720

Query: 721  ----------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDE 780
                                        GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDE
Sbjct: 721  SCNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGDSCFFSHDQSPSNSPSFESTLCLPEDE 780

Query: 781  KAHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDA 840
            KAHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDA
Sbjct: 781  KAHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDA 840

Query: 841  RKFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRM 875
            RKFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRM
Sbjct: 841  RKFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRM 900

BLAST of Cp4.1LG02g16410 vs. NCBI nr
Match: KAG7036665.1 (DExH-box ATP-dependent RNA helicase DExH8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1645 bits (4259), Expect = 0.0
Identity = 864/1004 (86.06%), Postives = 870/1004 (86.65%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 8    SSSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE
Sbjct: 68   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 128  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK
Sbjct: 188  FKELGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 247

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE
Sbjct: 248  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 307

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 308  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 367

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 368  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 427

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 428  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 487

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLH GILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 488  ILKFGDIGMLHVGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 547

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFH+       
Sbjct: 548  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHSLVHSSLN 607

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 608  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 667

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYP TNHVAGKLADVIKQMK                                   
Sbjct: 668  PYVASSYPLTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLNNGFDVHDGTALCVYFVNGS 727

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK
Sbjct: 728  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 787

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 788  AHALTLEKYFPKSGGCILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 847

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML
Sbjct: 848  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 907

BLAST of Cp4.1LG02g16410 vs. NCBI nr
Match: XP_022949129.1 (LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH8 [Cucurbita moschata])

HSP 1 Score: 1640 bits (4247), Expect = 0.0
Identity = 861/1004 (85.76%), Postives = 870/1004 (86.65%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 3    SSSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 62

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKI+FKTAGVLLEE
Sbjct: 63   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIIFKTAGVLLEE 122

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERS ESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 123  MRDRGLKALDYKVIVLDEVHERSAESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 182

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK
Sbjct: 183  FKELGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 242

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSL+SFKVYILHSSIDIE
Sbjct: 243  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLNSFKVYILHSSIDIE 302

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 303  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 362

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 363  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 422

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALK+TPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 423  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKRTPRGRYEPTYYGSLLASFSLSFDSSVL 482

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 483  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 542

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFH+       
Sbjct: 543  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHSLVHSSLN 602

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 603  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 662

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYPRTNHVAGKLADVIKQMK                                   
Sbjct: 663  PYVASSYPRTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLDNGFDVHDGTALCVYFVNGS 722

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNGDSCFFSHDQSPSNSPSF+STLCLPEDEK
Sbjct: 723  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGDSCFFSHDQSPSNSPSFKSTLCLPEDEK 782

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 783  AHALTLEKYFPKSGGSILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 842

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML
Sbjct: 843  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 902

BLAST of Cp4.1LG02g16410 vs. NCBI nr
Match: KAG6606962.1 (DExH-box ATP-dependent RNA helicase DExH8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1638 bits (4242), Expect = 0.0
Identity = 861/1004 (85.76%), Postives = 868/1004 (86.45%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 6    SSSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 65

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE
Sbjct: 66   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 125

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 126  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 185

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNG SPSTSSVEIK
Sbjct: 186  FKELGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGVSPSTSSVEIK 245

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE
Sbjct: 246  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 305

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 306  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 365

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 366  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 425

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 426  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 485

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLH GILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 486  ILKFGDIGMLHVGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 545

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSDEIQATTSTPS+DEEEWCSFH+       
Sbjct: 546  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDEIQATTSTPSEDEEEWCSFHSLVHSSLN 605

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 606  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 665

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYP TNHVAGKLADVIKQMK                                   
Sbjct: 666  PYVASSYPLTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLNNGFDVHDGTALCVYFVNGS 725

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK
Sbjct: 726  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 785

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 786  AHALTLEKYFPKSGGCILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 845

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML
Sbjct: 846  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 905

BLAST of Cp4.1LG02g16410 vs. NCBI nr
Match: XP_022998526.1 (DExH-box ATP-dependent RNA helicase DExH8 isoform X2 [Cucurbita maxima])

HSP 1 Score: 1622 bits (4200), Expect = 0.0
Identity = 856/1004 (85.26%), Postives = 864/1004 (86.06%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSST LSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 8    SSSSSSTSLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE
Sbjct: 68   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 128  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFE KVSYLEEVNELLGIESDLQSSNGFS STSSVEIK
Sbjct: 188  FKELGRGERVEVLAIPSSSQKTFFECKVSYLEEVNELLGIESDLQSSNGFSSSTSSVEIK 247

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE
Sbjct: 248  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 307

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVID+CRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 308  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDTCRSLQVFWDNNQKKDSAQVVWIS 367

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 368  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 427

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 428  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 487

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 488  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 547

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSD+IQATTSTPSKDEEEWCSFH+       
Sbjct: 548  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDKIQATTSTPSKDEEEWCSFHSLVHSSLN 607

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 608  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 667

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYPRTNHVAGKLADVIKQMK                                   
Sbjct: 668  PYVASSYPRTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLNNGFDVHDGTALCVYFVNGS 727

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNG SCFFSHDQSPSNSPSF+STLCLPEDEK
Sbjct: 728  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGVSCFFSHDQSPSNSPSFKSTLCLPEDEK 787

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 788  AHALTLEKYFPKSGGSILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 847

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFAS KENLVIEKLLL NFFDLLAVRML
Sbjct: 848  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASLKENLVIEKLLLHNFFDLLAVRML 907

BLAST of Cp4.1LG02g16410 vs. ExPASy TrEMBL
Match: A0A6J1GBX6 (LOW QUALITY PROTEIN: DExH-box ATP-dependent RNA helicase DExH8 OS=Cucurbita moschata OX=3662 GN=LOC111452575 PE=4 SV=1)

HSP 1 Score: 1640 bits (4247), Expect = 0.0
Identity = 861/1004 (85.76%), Postives = 870/1004 (86.65%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 3    SSSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 62

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKI+FKTAGVLLEE
Sbjct: 63   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIIFKTAGVLLEE 122

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERS ESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 123  MRDRGLKALDYKVIVLDEVHERSAESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 182

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK
Sbjct: 183  FKELGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 242

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSL+SFKVYILHSSIDIE
Sbjct: 243  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLNSFKVYILHSSIDIE 302

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 303  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 362

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 363  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 422

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALK+TPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 423  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKRTPRGRYEPTYYGSLLASFSLSFDSSVL 482

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 483  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 542

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFH+       
Sbjct: 543  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHSLVHSSLN 602

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 603  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 662

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYPRTNHVAGKLADVIKQMK                                   
Sbjct: 663  PYVASSYPRTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLDNGFDVHDGTALCVYFVNGS 722

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNGDSCFFSHDQSPSNSPSF+STLCLPEDEK
Sbjct: 723  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGDSCFFSHDQSPSNSPSFKSTLCLPEDEK 782

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 783  AHALTLEKYFPKSGGSILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 842

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML
Sbjct: 843  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 902

BLAST of Cp4.1LG02g16410 vs. ExPASy TrEMBL
Match: A0A6J1KAE9 (DExH-box ATP-dependent RNA helicase DExH8 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493135 PE=4 SV=1)

HSP 1 Score: 1622 bits (4200), Expect = 0.0
Identity = 856/1004 (85.26%), Postives = 864/1004 (86.06%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSST LSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 8    SSSSSSTSLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE
Sbjct: 68   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 128  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFE KVSYLEEVNELLGIESDLQSSNGFS STSSVEIK
Sbjct: 188  FKELGRGERVEVLAIPSSSQKTFFECKVSYLEEVNELLGIESDLQSSNGFSSSTSSVEIK 247

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE
Sbjct: 248  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 307

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVID+CRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 308  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDTCRSLQVFWDNNQKKDSAQVVWIS 367

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 368  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 427

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 428  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 487

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 488  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIPLGFKEM 547

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DKIRVEYLNKLVKSD+IQATTSTPSKDEEEWCSFH+       
Sbjct: 548  ALIGNLHAFQFWERVYKDKIRVEYLNKLVKSDKIQATTSTPSKDEEEWCSFHSLVHSSLN 607

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 661
                  EDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL
Sbjct: 608  HVSEMYEDIIHTLHQFRPRFLGMCDILRSSNTPQFQHSCILKCHENGVDQSSESRTCASL 667

Query: 662  PYVASSYPRTNHVAGKLADVIKQMK----------------------------------- 721
            PYVASSYPRTNHVAGKLADVIKQMK                                   
Sbjct: 668  PYVASSYPRTNHVAGKLADVIKQMKVIYGKEPNNQSLSSLNNGFDVHDGTALCVYFVNGS 727

Query: 722  ---------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLPEDEK 781
                                       GCRNG SCFFSHDQSPSNSPSF+STLCLPEDEK
Sbjct: 728  CNRGDQCLFSHSLQSRRATCKFFFSLQGCRNGVSCFFSHDQSPSNSPSFKSTLCLPEDEK 787

Query: 782  AHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAALKDAR 841
            AHALTLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+SRSDVYDAALKDAR
Sbjct: 788  AHALTLEKYFPKSGGSILVMDDAGFHFSSNLARHCDPSKIICTTNLSRSDVYDAALKDAR 847

Query: 842  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLAVRML 875
            KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFAS KENLVIEKLLL NFFDLLAVRML
Sbjct: 848  KFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASLKENLVIEKLLLHNFFDLLAVRML 907

BLAST of Cp4.1LG02g16410 vs. ExPASy TrEMBL
Match: A0A6J1KEJ9 (DExH-box ATP-dependent RNA helicase DExH8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493135 PE=4 SV=1)

HSP 1 Score: 1611 bits (4172), Expect = 0.0
Identity = 856/1032 (82.95%), Postives = 864/1032 (83.72%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSST LSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED
Sbjct: 8    SSSSSSTSLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE
Sbjct: 68   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY
Sbjct: 128  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEIK 241
            FKE+GRGERVEVLAIPSSSQKTFFE KVSYLEEVNELLGIESDLQSSNGFS STSSVEIK
Sbjct: 188  FKELGRGERVEVLAIPSSSQKTFFECKVSYLEEVNELLGIESDLQSSNGFSSSTSSVEIK 247

Query: 242  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 301
            PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE
Sbjct: 248  PEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSIDIE 307

Query: 302  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWIS 361
            QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVID+CRSLQVFWDNNQKKDSAQVVWIS
Sbjct: 308  QALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDTCRSLQVFWDNNQKKDSAQVVWIS 367

Query: 362  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 421
            KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN
Sbjct: 368  KSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKAIN 427

Query: 422  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 481
            DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL
Sbjct: 428  DPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVL 487

Query: 482  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL------------------------ 541
            ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                        
Sbjct: 488  ILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLVFLDCYLLGFPYLTLACILINFES 547

Query: 542  ---------------------------------------------DKIRVEYLNKLVKSD 601
                                                         DKIRVEYLNKLVKSD
Sbjct: 548  YLIQYAEYIKSYFDGESIDTIPLGFKEMALIGNLHAFQFWERVYKDKIRVEYLNKLVKSD 607

Query: 602  EIQATTSTPSKDEEEWCSFHN-------------EDIIHTLHQFRPRFLGMCDILRSSNT 661
            +IQATTSTPSKDEEEWCSFH+             EDIIHTLHQFRPRFLGMCDILRSSNT
Sbjct: 608  KIQATTSTPSKDEEEWCSFHSLVHSSLNHVSEMYEDIIHTLHQFRPRFLGMCDILRSSNT 667

Query: 662  PQFQHSCILKCHENGVDQSSESRTCASLPYVASSYPRTNHVAGKLADVIKQMK------- 721
            PQFQHSCILKCHENGVDQSSESRTCASLPYVASSYPRTNHVAGKLADVIKQMK       
Sbjct: 668  PQFQHSCILKCHENGVDQSSESRTCASLPYVASSYPRTNHVAGKLADVIKQMKVIYGKEP 727

Query: 722  -------------------------------------------------------GCRNG 781
                                                                   GCRNG
Sbjct: 728  NNQSLSSLNNGFDVHDGTALCVYFVNGSCNRGDQCLFSHSLQSRRATCKFFFSLQGCRNG 787

Query: 782  DSCFFSHDQSPSNSPSFESTLCLPEDEKAHALTLEKYFPKSGGYILVMDDAGFHFSSNLA 841
             SCFFSHDQSPSNSPSF+STLCLPEDEKAHALTLEKYFPKSGG ILVMDDAGFHFSSNLA
Sbjct: 788  VSCFFSHDQSPSNSPSFKSTLCLPEDEKAHALTLEKYFPKSGGSILVMDDAGFHFSSNLA 847

Query: 842  RHCDPSKIICTTNISRSDVYDAALKDARKFWELSHPDETIISSGKNQIPWFDVKCILWFP 875
            RHCDPSKIICTTN+SRSDVYDAALKDARKFWELSHPDETIISSGKNQIPWFDVKCILWFP
Sbjct: 848  RHCDPSKIICTTNLSRSDVYDAALKDARKFWELSHPDETIISSGKNQIPWFDVKCILWFP 907

BLAST of Cp4.1LG02g16410 vs. ExPASy TrEMBL
Match: A0A1S3BZ58 (DExH-box ATP-dependent RNA helicase DExH8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494773 PE=4 SV=1)

HSP 1 Score: 1447 bits (3745), Expect = 0.0
Identity = 764/1001 (76.32%), Postives = 812/1001 (81.12%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSS+  S SP  FSALPVMSL+ERIVEKIR+NR+TLIVGETGCGKSSQIPQFLL+ED
Sbjct: 6    SSSSSSSSSSPSPLVFSALPVMSLRERIVEKIRQNRVTLIVGETGCGKSSQIPQFLLEED 65

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSE+SKIVFKTAGVLLEE
Sbjct: 66   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSEKSKIVFKTAGVLLEE 125

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGL AL+YKVIVLDEVHERSVESDLVLVCVKQFL K+HDLRVVLMSATADIGRYRDY
Sbjct: 126  MRDRGLNALNYKVIVLDEVHERSVESDLVLVCVKQFLSKHHDLRVVLMSATADIGRYRDY 185

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSS---NGFSPSTSSV 241
            FK++GRGERVEVLAIP+S+QK+FFERKVSYLEEV ELLGIESDLQSS   NGFSP TS V
Sbjct: 186  FKDLGRGERVEVLAIPNSNQKSFFERKVSYLEEVTELLGIESDLQSSRYCNGFSPYTSPV 245

Query: 242  EIKPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSI 301
            EIKPEVH+LIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKS SSFKVYILHSSI
Sbjct: 246  EIKPEVHRLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSHSSFKVYILHSSI 305

Query: 302  DIEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVV 361
            DIEQALTAMRIWKSHRK+ILATNIAESSVTIPKVAYVIDSCRSLQV+WDNNQKKDSAQVV
Sbjct: 306  DIEQALTAMRIWKSHRKIILATNIAESSVTIPKVAYVIDSCRSLQVYWDNNQKKDSAQVV 365

Query: 362  WISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESK 421
            WISKSQAEQRRGRTGRTCDGQVYRLV RSFYH FEDFERP ILRLSLRQQVLLICSTESK
Sbjct: 366  WISKSQAEQRRGRTGRTCDGQVYRLVTRSFYHNFEDFERPDILRLSLRQQVLLICSTESK 425

Query: 422  AINDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDS 481
            AINDPTVLLQK LDPP  NVVEDAL+LLV M+ALK++PRGRYEPTYYGSLLASFSLSFDS
Sbjct: 426  AINDPTVLLQKTLDPPAANVVEDALSLLVHMQALKRSPRGRYEPTYYGSLLASFSLSFDS 485

Query: 482  SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL--------------------- 541
            SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                     
Sbjct: 486  SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIQLGF 545

Query: 542  --------------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN---- 601
                                DKIRVEYLNKL+  ++ Q   S PSK+EEEWCSFH+    
Sbjct: 546  KEMALLGNLHAFHFWERVYKDKIRVEYLNKLLNPNKTQTIKSPPSKNEEEWCSFHSLVHS 605

Query: 602  ---------EDIIHTLHQFRPRFLGMCDILRSSNTP-QFQHSCILKCHENGVDQSSESRT 661
                     EDIIHTLHQFRPRFLGMCDILRSS  P QFQHSC+LK  ENG DQSSESRT
Sbjct: 606  SLNHVSEMYEDIIHTLHQFRPRFLGMCDILRSSYAPTQFQHSCVLKRLENGDDQSSESRT 665

Query: 662  CASLPYVASSYPRTNHVAGKLADVIKQMK------------------------------- 721
            C SLPYVASSY RTN VAGKLADVIKQMK                               
Sbjct: 666  CVSLPYVASSYSRTNQVAGKLADVIKQMKVFYAKEEPNNHSLSSINDGFNVHNGTSICVY 725

Query: 722  --------------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCL 781
                                            GCRNGDSC FSHDQSPS S SF+STLCL
Sbjct: 726  FLNGSCNRGSRCIFSHSLQSKRATCKFFFSLQGCRNGDSCLFSHDQSPSKSLSFKSTLCL 785

Query: 782  PEDEKAHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAA 841
            PED  AHA TLEKYFPKSGG ILVMDDAGFHFSSNLARHCDPSKIICTTN+S SD+YDA+
Sbjct: 786  PEDGIAHASTLEKYFPKSGGCILVMDDAGFHFSSNLARHCDPSKIICTTNLSHSDIYDAS 845

Query: 842  LKDARKFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLL 867
            L DA+KFWELSHPDETIIS+G+NQIPW+DVKCILWFPRFASSKENL IEK+LLQNFFDLL
Sbjct: 846  LNDAKKFWELSHPDETIISNGENQIPWYDVKCILWFPRFASSKENLDIEKILLQNFFDLL 905

BLAST of Cp4.1LG02g16410 vs. ExPASy TrEMBL
Match: A0A0A0KV69 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G001760 PE=4 SV=1)

HSP 1 Score: 1435 bits (3714), Expect = 0.0
Identity = 754/997 (75.63%), Postives = 806/997 (80.84%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSS+  S SP  FSALPVMSL+ERIVEKIR+NR+TLIVGETGCGKSSQIPQFLL+ED
Sbjct: 5    SSSSSSSSSSPSPLVFSALPVMSLRERIVEKIRQNRVTLIVGETGCGKSSQIPQFLLEED 64

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            MGPILCTQPRRFAVVAIANMVARAR CNVGEEVGYHIGHSKHSSE+SKIVFKTAGVLLEE
Sbjct: 65   MGPILCTQPRRFAVVAIANMVARARKCNVGEEVGYHIGHSKHSSEKSKIVFKTAGVLLEE 124

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            MRDRGL AL+YKVIVLDEVHERSVESDLVLVCVKQFL K+HDLRVVLMSATADIGRYRDY
Sbjct: 125  MRDRGLNALNYKVIVLDEVHERSVESDLVLVCVKQFLSKHHDLRVVLMSATADIGRYRDY 184

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSS---NGFSPSTSSV 241
            FK++GRGERVEVLAIP+S+QK+FFERKVSYLEEV ELLGIESDLQSS   NGFSP  S  
Sbjct: 185  FKDLGRGERVEVLAIPNSNQKSFFERKVSYLEEVTELLGIESDLQSSRYCNGFSPCASPA 244

Query: 242  EIKPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKSLSSFKVYILHSSI 301
            EIK EVH+LIHNLLLHIHKNESDIEKSIL+FLPTYYSLEQQWHLLKS SSFKVYILHSSI
Sbjct: 245  EIKSEVHRLIHNLLLHIHKNESDIEKSILVFLPTYYSLEQQWHLLKSHSSFKVYILHSSI 304

Query: 302  DIEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVV 361
            DIEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQV+WDNNQKKDS QVV
Sbjct: 305  DIEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVYWDNNQKKDSPQVV 364

Query: 362  WISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESK 421
            WISKSQAEQRRGRTGRTCDGQVYRLV RSFYH FEDFERP ILRLSLRQQVLLICSTESK
Sbjct: 365  WISKSQAEQRRGRTGRTCDGQVYRLVTRSFYHNFEDFERPDILRLSLRQQVLLICSTESK 424

Query: 422  AINDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDS 481
            AINDP VLLQK LDPPD NVVEDAL+LLV M+ALK++PRGRYEPTYYGSLLASFSLSFDS
Sbjct: 425  AINDPAVLLQKTLDPPDANVVEDALSLLVNMQALKRSPRGRYEPTYYGSLLASFSLSFDS 484

Query: 482  SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL--------------------- 541
            SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL                     
Sbjct: 485  SVLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNLYAEYIKSYFDGESIDTIQLGF 544

Query: 542  --------------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN---- 601
                                DKIRVEYLNKLV  ++ Q TTS PSK+EEEWCSFH+    
Sbjct: 545  KEMALLGNLHAFHFWERVYKDKIRVEYLNKLVNPNKTQTTTSPPSKNEEEWCSFHSLVHS 604

Query: 602  ---------EDIIHTLHQFRPRFLGMCDILRSSNTP-QFQHSCILKCHENGVDQSSESRT 661
                     EDIIHTLHQFRPRFLGMCDILRSS  P QFQH C+LKC ENG DQSSESRT
Sbjct: 605  SLNHVSEMYEDIIHTLHQFRPRFLGMCDILRSSYAPTQFQHLCVLKCLENGDDQSSESRT 664

Query: 662  CASLPYVASSYPRTNHVAGKLADVIKQMK------------------------------- 721
            C S+PYVASSY RTN VAGKLADVIKQMK                               
Sbjct: 665  CVSVPYVASSYSRTNQVAGKLADVIKQMKVFYAKEEPNNHSLSSMNNGFNDNGTSLCVYF 724

Query: 722  -------------------------------GCRNGDSCFFSHDQSPSNSPSFESTLCLP 781
                                           GCRNGDSC FSHDQSPS S SF+STLCLP
Sbjct: 725  LNGSCNRGSQCLFSHSLQSKRATCKFFFSLQGCRNGDSCLFSHDQSPSKSLSFKSTLCLP 784

Query: 782  EDEKAHALTLEKYFPKSGGYILVMDDAGFHFSSNLARHCDPSKIICTTNISRSDVYDAAL 841
            ED  AHA TLEKYFPKSGG ILVMDDAGFHFSSNLARHC+PSKIICTTN+S SD+YD++L
Sbjct: 785  EDGIAHASTLEKYFPKSGGCILVMDDAGFHFSSNLARHCEPSKIICTTNLSHSDIYDSSL 844

Query: 842  KDARKFWELSHPDETIISSGKNQIPWFDVKCILWFPRFASSKENLVIEKLLLQNFFDLLA 864
             DA+K WELSHPDETIIS+G+NQIPW+DVKCILWFPRFASSKENL IEK+LLQNFFDLLA
Sbjct: 845  NDAKKIWELSHPDETIISNGENQIPWYDVKCILWFPRFASSKENLDIEKILLQNFFDLLA 904

BLAST of Cp4.1LG02g16410 vs. TAIR 10
Match: AT2G47680.1 (zinc finger (CCCH type) helicase family protein )

HSP 1 Score: 931.4 bits (2406), Expect = 5.4e-271
Identity = 508/1007 (50.45%), Postives = 663/1007 (65.84%), Query Frame = 0

Query: 2    ASSSSSTLLSSSPFDFSALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDED 61
            +SSSSS  L     +F++LP+M++K RI++KI +NR+TLIVGE GCGKSSQ+PQFLL+ +
Sbjct: 8    SSSSSSESLPLPSSNFASLPIMAMKRRIIDKILENRVTLIVGEPGCGKSSQVPQFLLEAN 67

Query: 62   MGPILCTQPRRFAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEE 121
            M PILCTQPRRFAVVA+A MVA++RN ++G E+GYHIGHSK  +E SKI+FKTAGVLL+E
Sbjct: 68   MAPILCTQPRRFAVVAVAKMVAKSRNSDLGGEIGYHIGHSKILTEGSKILFKTAGVLLDE 127

Query: 122  MRDRGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDY 181
            M D+GL AL YKVI+LDEVHERSVESDLVLVCVKQFL+KN+DLRVVLMSATADI RYRDY
Sbjct: 128  MLDKGLNALKYKVIILDEVHERSVESDLVLVCVKQFLMKNNDLRVVLMSATADITRYRDY 187

Query: 182  FKEIGRGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQS-SNGFSPSTSSVEI 241
            FKE+GRGERVEV+AIPS  Q+T F+R+V YLE+V  LLG+ SDL +   G SPS++  EI
Sbjct: 188  FKELGRGERVEVVAIPSPDQRTIFQRRVLYLEQVAGLLGVSSDLSAYCPGPSPSSADTEI 247

Query: 242  KPEVHKLIHNLLLHIHKNESDIEKSILIFLPTYYSLEQQWHLLKS-LSSFKVYILHSSID 301
            KPE+  LIH+L+L+IH+ E DIEKSIL+FLPTYYSLEQQ+H L+   +SF+V+ILH SID
Sbjct: 248  KPELQNLIHDLILYIHEKEPDIEKSILVFLPTYYSLEQQYHQLEPFFASFEVHILHRSID 307

Query: 302  IEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVW 361
             EQAL AM+I +S RKVILATNIAESSVTIPKVAYVIDSCRSLQVFWD ++K+D+ Q+VW
Sbjct: 308  TEQALAAMKICRSRRKVILATNIAESSVTIPKVAYVIDSCRSLQVFWDPSRKRDAVQLVW 367

Query: 362  ISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFERPAILRLSLRQQVLLICSTESKA 421
            +S+SQAEQRRGRTGRTCDG+VYRLVP +F++K E+ E P+IL+LSLRQQVL IC TES+A
Sbjct: 368  VSRSQAEQRRGRTGRTCDGEVYRLVPSAFFNKLEEHEPPSILKLSLRQQVLHICCTESRA 427

Query: 422  INDPTVLLQKALDPPDGNVVEDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSS 481
            IND   LL KA+DPPD +VV+DAL +L+ ++AL+K+PRGRYEPT+YG LLASF LSFD+S
Sbjct: 428  INDANALLAKAMDPPDPDVVDDALRMLLSIQALRKSPRGRYEPTFYGRLLASFPLSFDAS 487

Query: 482  VLILKFGDIGMLHEGILLGILMDTQPLPVLRPFGENNL---------------------- 541
            +L++KFG++GML +GILLG+LMDT PLP+  PFG+++L                      
Sbjct: 488  ILVVKFGEMGMLRQGILLGVLMDTLPLPIHHPFGDDSLFLEYVDHYFGGSKTISGGRREM 547

Query: 542  -----------------DKIRVEYLNKLVKSDEIQATTSTPSKDEEEWCSFHN------- 601
                             DK R+E L +L+  ++ +       + E+EWC FHN       
Sbjct: 548  VLMANFCAFQFWQRVFKDKHRLENLKQLLSKEKDKDLKLMFPEIEKEWCDFHNIAQSSFY 607

Query: 602  ------EDIIHTLHQFRPRFLGMCDILRSSNTP-QFQHSCILKC--------HENGVDQS 661
                  ED + + H+FRP+F+   D   +   P +F H+C ++C        H   VD +
Sbjct: 608  HVSELYEDTLSSFHRFRPQFISSSDSQPTYYNPYEFDHTCYIECQPSEDKYLHSEDVDNN 667

Query: 662  S---ESRTCASLPYVASSYPRTNHVAGKLADVIKQMK----------------------- 721
                E R C S+P+V  +  + N +A  +A +IK+++                       
Sbjct: 668  QPPPEVRKCVSVPFVPPNAFQANAIAENMASIIKEIRTQCTPSESDNGHGALEPEDYVEY 727

Query: 722  ----------------------------------------GCRNGDSCFFSHDQSPSNSP 781
                                                    GCRNG+SC FSH      + 
Sbjct: 728  GEAPVCVYFLNGYCNRGGQCTFTHTLQSTRPACKFFASSQGCRNGESCLFSHAMRRRTTS 787

Query: 782  SFESTLCLPEDEKAHALTLEKYFPKSG-GYILVMDDAGFHFSSNLARHCDPSKIICTTNI 841
                  CLPE++ +    L   FP S  G ILV DD+  HF+S++A      +I+ T++ 
Sbjct: 788  YLPPPQCLPEEDGSSTSPLLDLFPTSSEGCILVFDDSDMHFTSSIANRYPSWRILSTSSS 847

Query: 842  SRSDVYDAALKDARKFWELSHPDETIISSG--KNQIPWFDVKCILWFPRFASSKENLVIE 863
            S +   D++L D R FW L+HP +TIIS    +N IPW +VKC+LWF    S  +    +
Sbjct: 848  SETLFCDSSLADTRIFWGLNHPYQTIISKAGRENPIPWNEVKCVLWFLNPDSYADTPEKQ 907

BLAST of Cp4.1LG02g16410 vs. TAIR 10
Match: AT2G35920.1 (RNA helicase family protein )

HSP 1 Score: 212.2 bits (539), Expect = 1.7e-54
Identity = 149/488 (30.53%), Postives = 249/488 (51.02%), Query Frame = 0

Query: 20  LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDEDMGP-------ILCTQPRR 79
           LP   +KE  +  + +N++ ++ GETGCGK++Q+PQF+L+E++         I+CTQPRR
Sbjct: 228 LPAFKMKEEFLNSVSQNQVLVVSGETGCGKTTQLPQFILEEEISSLRGADCNIICTQPRR 287

Query: 80  FAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEEM-RDRGLKALD 139
            + +++A+ ++  R  ++GE VGY I      S++++++F T GVLL  +  D  L  + 
Sbjct: 288 ISAISVASRISAERGESIGESVGYQIRLESKRSDQTRLLFCTTGVLLRRLIEDPNLTNVS 347

Query: 140 YKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYF--------- 199
           +  +++DE+HER +  D +L+ ++  L +  DLR++LMSAT +   +  YF         
Sbjct: 348 H--LLVDEIHERGMNEDFLLIILRDLLPRRPDLRLILMSATINADMFSTYFGNSPTMHIP 407

Query: 200 -----------KEIGRGERVEVLAIPSSS-QKTFFERKVSYLEEVNELLGIESDLQSSNG 259
                      +++    R  + +  S + Q +   R+     + ++L  +  D+  ++ 
Sbjct: 408 GFTFPVAELFLEDVLEKSRYNIKSSDSGNYQGSSRGRRRESESKKDDLTTLFEDIDINSH 467

Query: 260 FSPSTSSVEIKPEVHK-------LIHNLLLHIHKNESDIEKSILIFLPTYYSLEQ----- 319
           +   +S+     E          L+   + HI + E     +IL+FL  +  + +     
Sbjct: 468 YKSYSSATRNSLEAWSGAQIDVDLVEATIEHICRLEGG--GAILVFLTGWDEISKLLEKI 527

Query: 320 -QWHLLKSLSSFKVYILHSSI-DIEQALTAMRIWKSHRKVILATNIAESSVTIPKVAYVI 379
              + L   S F V  LH S+  + Q     R   + RK++LATNIAESS+TI  V YV+
Sbjct: 528 NMNNFLGDSSKFLVLPLHGSMPTVNQREIFDRPPPNKRKIVLATNIAESSITIDDVVYVV 587

Query: 380 DSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQVYRLVPRSFYHKFEDFE 439
           D  ++ +  +D   K       WISK+ A QRRGR GR   G  YRL P+  Y  F  ++
Sbjct: 588 DCGKAKETSYDALNKVACLLPSWISKASAHQRRGRAGRVQAGVCYRLYPKVIYDAFPQYQ 647

Query: 440 RPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDPPDGNVVEDALNLLVRMKAL---- 461
            P I+R  L++  L I S +  +I      L KAL PPD   VE+A+ LL  + AL    
Sbjct: 648 LPEIIRTPLQELCLHIKSLQVGSIGS---FLAKALQPPDALAVENAIELLKTIGALNDVE 707

BLAST of Cp4.1LG02g16410 vs. TAIR 10
Match: AT1G48650.1 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 199.5 bits (506), Expect = 1.1e-50
Identity = 152/493 (30.83%), Postives = 244/493 (49.49%), Query Frame = 0

Query: 20  LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDEDMG-------PILCTQPRR 79
           LP    K+ +++ I  N++ ++ GETGCGK++Q+PQ++L+ ++         I+CTQPRR
Sbjct: 299 LPAYKEKDALLKAIAANQVVVVSGETGCGKTTQLPQYILESEIEAARGATCSIICTQPRR 358

Query: 80  FAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEEMR-DRGLKALD 139
            + ++++  VA  R   +GE VGY +         ++++F T GVLL  +  DR LK + 
Sbjct: 359 ISAISVSERVAAERGEQIGESVGYKVRLEGMRGRDTRLLFCTTGVLLRRLLVDRSLKGVT 418

Query: 140 YKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYFKEIG----- 199
           +  +V+DE+HER +  D +L+ +K  L +  DL+++LMSAT +   +  YF         
Sbjct: 419 H--VVVDEIHERGMNEDFLLIVLKDLLPRRPDLKLILMSATLNAELFSSYFGGAPAMHIP 478

Query: 200 ------RGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEI 259
                 R   +E     S  + T + +   Y EE  +   ++   Q     S  +S+VE 
Sbjct: 479 GFTYPVRAHFLEDYLETSGYRLTTYNQIDDYGEE--KTWKMQKQAQFKKRKSLISSAVED 538

Query: 260 KPEV-----------------------HKLIHNLLLHIHKNESDIEKSILIFLPTYYSLE 319
             E                          LI N+L HI K E     ++L+F+  +  + 
Sbjct: 539 ALEAADFKGYNFRTRDSLSCWSPDSIGFNLIENVLCHIVKGER--PGAVLVFMTGWDDIN 598

Query: 320 QQWHLLKSLSSF----KVYIL--HSSI-DIEQALTAMRIWKSHRKVILATNIAESSVTIP 379
              + L++ S      KV +L  H S+   EQ L   R  +  RK++LATN+AE+S+TI 
Sbjct: 599 SLKNQLEAHSLLGDPNKVLLLACHGSMASSEQRLIFDRPPEGIRKIVLATNMAETSITIN 658

Query: 380 KVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQVYRLVPRSFYH 439
            V YVID  ++ +  +D           WISK+ A QRRGR GR   G+ Y L PR  Y 
Sbjct: 659 DVVYVIDCGKAKETSYDALNNTPCLLPSWISKAAARQRRGRAGRVMPGECYHLYPRCVYE 718

Query: 440 KFEDFERPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDPPDGNVVEDALNLLVRMK 460
            F D+++P +LR  L+   L I S    +I++    L +AL PP+   V++A+  L  + 
Sbjct: 719 AFADYQQPELLRTPLQSLCLQIKSLGLGSISE---FLSRALQPPEALSVQNAVEYLKIIG 778

BLAST of Cp4.1LG02g16410 vs. TAIR 10
Match: AT1G48650.2 (DEA(D/H)-box RNA helicase family protein )

HSP 1 Score: 199.5 bits (506), Expect = 1.1e-50
Identity = 152/493 (30.83%), Postives = 244/493 (49.49%), Query Frame = 0

Query: 20  LPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDEDMG-------PILCTQPRR 79
           LP    K+ +++ I  N++ ++ GETGCGK++Q+PQ++L+ ++         I+CTQPRR
Sbjct: 299 LPAYKEKDALLKAIAANQVVVVSGETGCGKTTQLPQYILESEIEAARGATCSIICTQPRR 358

Query: 80  FAVVAIANMVARARNCNVGEEVGYHIGHSKHSSERSKIVFKTAGVLLEEMR-DRGLKALD 139
            + ++++  VA  R   +GE VGY +         ++++F T GVLL  +  DR LK + 
Sbjct: 359 ISAISVSERVAAERGEQIGESVGYKVRLEGMRGRDTRLLFCTTGVLLRRLLVDRSLKGVT 418

Query: 140 YKVIVLDEVHERSVESDLVLVCVKQFLLKNHDLRVVLMSATADIGRYRDYFKEIG----- 199
           +  +V+DE+HER +  D +L+ +K  L +  DL+++LMSAT +   +  YF         
Sbjct: 419 H--VVVDEIHERGMNEDFLLIVLKDLLPRRPDLKLILMSATLNAELFSSYFGGAPAMHIP 478

Query: 200 ------RGERVEVLAIPSSSQKTFFERKVSYLEEVNELLGIESDLQSSNGFSPSTSSVEI 259
                 R   +E     S  + T + +   Y EE  +   ++   Q     S  +S+VE 
Sbjct: 479 GFTYPVRAHFLEDYLETSGYRLTTYNQIDDYGEE--KTWKMQKQAQFKKRKSLISSAVED 538

Query: 260 KPEV-----------------------HKLIHNLLLHIHKNESDIEKSILIFLPTYYSLE 319
             E                          LI N+L HI K E     ++L+F+  +  + 
Sbjct: 539 ALEAADFKGYNFRTRDSLSCWSPDSIGFNLIENVLCHIVKGER--PGAVLVFMTGWDDIN 598

Query: 320 QQWHLLKSLSSF----KVYIL--HSSI-DIEQALTAMRIWKSHRKVILATNIAESSVTIP 379
              + L++ S      KV +L  H S+   EQ L   R  +  RK++LATN+AE+S+TI 
Sbjct: 599 SLKNQLEAHSLLGDPNKVLLLACHGSMASSEQRLIFDRPPEGIRKIVLATNMAETSITIN 658

Query: 380 KVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQVYRLVPRSFYH 439
            V YVID  ++ +  +D           WISK+ A QRRGR GR   G+ Y L PR  Y 
Sbjct: 659 DVVYVIDCGKAKETSYDALNNTPCLLPSWISKAAARQRRGRAGRVMPGECYHLYPRCVYE 718

Query: 440 KFEDFERPAILRLSLRQQVLLICSTESKAINDPTVLLQKALDPPDGNVVEDALNLLVRMK 460
            F D+++P +LR  L+   L I S    +I++    L +AL PP+   V++A+  L  + 
Sbjct: 719 AFADYQQPELLRTPLQSLCLQIKSLGLGSISE---FLSRALQPPEALSVQNAVEYLKIIG 778

BLAST of Cp4.1LG02g16410 vs. TAIR 10
Match: AT1G58050.1 (RNA helicase family protein )

HSP 1 Score: 193.4 bits (490), Expect = 8.0e-49
Identity = 170/587 (28.96%), Postives = 285/587 (48.55%), Query Frame = 0

Query: 18   SALPVMSLKERIVEKIRKNRITLIVGETGCGKSSQIPQFLLDE--DMG-----PILCTQP 77
            +ALP+  +K+ I++K+++  + ++ GETG GK++Q+PQF+LD+  D G      I+CTQP
Sbjct: 595  AALPISEVKKDILQKLKEKDVLVVCGETGSGKTTQVPQFILDDMIDSGHGGYCNIICTQP 654

Query: 78   RRFAVVAIANMVARARNCNV-----GEEVGYHIGHSKHSSERSKIVFKTAGVLLEEM-RD 137
            R    + +A  VA  R C          V Y + H    S++++++F T G+LL ++  D
Sbjct: 655  R---AITVAQRVADER-CEPPPGFDNSVVAYQVRHQNARSDKTRLLFCTTGILLRKLVGD 714

Query: 138  RGLKALDYKVIVLDEVHERSVESDLVLVCVKQFLLKNH------DLRVVLMSATADIGRY 197
              LK  D   I++DEVHERS+  D +L+ +K  + K         L+V+LMSAT D  ++
Sbjct: 715  TTLK--DVTHIIVDEVHERSLMGDFLLIILKSLIEKQSWDNALPKLKVILMSATVDAHQF 774

Query: 198  RDYFKEI------GRGERVEVLAIPSSSQKTFF------ERKVSYLEEVNELLGI----- 257
              YF +       GR   V    +    ++T +         +S    + + LG      
Sbjct: 775  SRYFGQCPIITAQGRTHPVTTYFLEDIYERTKYLLASDSPAALSSDTSITDKLGSVNVPR 834

Query: 258  -----------ESDLQSSNGFSPSTSSVEIKPEV---HKLIHNLLLHIHKNESDIEKSIL 317
                       +S L S +  + S  S++        + L+  L+ HI  +++  E +IL
Sbjct: 835  GKKNLMLAGWGDSYLVSEDSLNTSYDSIKYIASAVVDYDLLEELICHI--DDTCEEGAIL 894

Query: 318  IFLPTYYSLEQQWHLLKSLSSFK------VYILHSSI-DIEQALTAMRIWKSHRKVILAT 377
            +FLP    +    + L +   F+      +  LHSSI   EQ    +R  K  RKVI+AT
Sbjct: 895  VFLPGMSEINMLLNRLAASYRFRGASGDWLLPLHSSIASTEQKKVFLRPPKGIRKVIIAT 954

Query: 378  NIAESSVTIPKVAYVIDSCRSLQVFWDNNQKKDSAQVVWISKSQAEQRRGRTGRTCDGQV 437
            NIAE+S+TI  V YVIDS +  +  ++ ++K  S    W+SK+ A QR GR GR   G  
Sbjct: 955  NIAETSITIEDVVYVIDSGKHKENRYNPHKKLSSMVEDWVSKANARQRMGRAGRVKPGHC 1014

Query: 438  YRLVPRSFYHKFEDFERPAILRLSLRQQVLLIC-STESKAINDPTVLLQKALDPPDGNVV 497
            + L  R   H+FE   RP  +   LR  ++ +C   +   +      L KAL+PP  + +
Sbjct: 1015 FSLYTR---HRFEKLMRPYQVPEMLRVPLVELCLHIKLLGLGQIKPFLSKALEPPSESAI 1074

Query: 498  EDALNLLVRMKALKKTPRGRYEPTYYGSLLASFSLSFDSSVLILKFGDIGMLHEGILLGI 547
              A+ LL ++ AL+    G  E T  G  LA   +      ++L  G  G L   + +  
Sbjct: 1075 NSAILLLHKVGALE----GDEELTPLGHHLAKLPVDLLIGKMLLYGGIFGCLSPILSIAA 1134