Cp4.1LG02g15870 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g15870 vs. ExPASy Swiss-Prot
Match: Q9LJD8 (MAP3K epsilon protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=M3KE1 PE=1 SV=1)

HSP 1 Score: 1713.0 bits (4435), Expect = 0.0e+00
Identity = 977/1505 (64.92%), Postives = 1098/1505 (72.96%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            M+RQ+  + FHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LN IM                       QEIDLLKNLNHKNIVKYLGS KTK+HLHIILE
Sbjct: 61   LNTIM-----------------------QEIDLLKNLNHKNIVKYLGSSKTKTHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS ANIIKPNKFGPFPESLVA+YI+QVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSLANIIKPNKFGPFPESLVAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKL EADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLNEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D +PPIPDSLSPDITDFLRQCFKKD+RQRPDAKTLLSHPW++N RRAL S
Sbjct: 301  PMPALFRIVQDDNPPIPDSLSPDITDFLRQCFKKDSRQRPDAKTLLSHPWIRNSRRALQS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGT++  + + +  +E  +   Q++ ES S E   I+    K DS+ +L   V   
Sbjct: 361  SLRHSGTIKYMK-EATASSEKDDEGSQDAAESLSGENVGIS----KTDSKSKLPL-VGVS 420

Query: 581  TSKSQKHFAS-GHFDEEG-EGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHES 640
            + +S+K  ++     EEG +  E+D   DQVPTLSI +  S   G+       P +  + 
Sbjct: 421  SFRSEKDQSTPSDLGEEGTDNSEDDIMSDQVPTLSIHEKSSDAKGT-------PQDVSDF 480

Query: 641  HGRA-HDEVIMNGGVPSAELRKVASR-KYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKM 700
            HG++   E   N    ++E RK  S  K+  KE S     +S  FG +G +   +K  K 
Sbjct: 481  HGKSERGETPENLVTETSEARKNTSAIKHVGKELSIPVDQTSHSFGRKGEERGIRKAVKT 540

Query: 701  SIASGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGK 760
              +  GNEL++FSD PGDASL DLF PLDK+   +  EASTS+ T   N G+ PV + GK
Sbjct: 541  PSSVSGNELARFSDPPGDASLHDLFHPLDKVSEGKPNEASTSMPTSNVNQGDSPVADGGK 600

Query: 761  NDLATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDV-DIDGLVFDEKLPGENLYP 820
            NDLATKLRATIAQKQME E G  N G DL RL+MGVL DDV DIDGLVFDEK+P ENL+P
Sbjct: 601  NDLATKLRATIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEKVPAENLFP 660

Query: 821  LPAVEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPK 880
            L AVEF RLV SLRPDE ED IVS+C KL+A+F  RPEQK+V+VTQHG LPL +LL++PK
Sbjct: 661  LQAVEFSRLVSSLRPDESEDAIVSSCQKLVAMFRQRPEQKVVFVTQHGFLPLMDLLDIPK 720

Query: 881  TRITCSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVP--DRPREVRMEAAYFFQQLC 940
            +R+ C+VLQLIN+I+KDN DFQENACLVGLIP+VM FA P  DR RE+R EAAYF QQLC
Sbjct: 721  SRVICAVLQLINEIIKDNTDFQENACLVGLIPVVMSFAGPERDRSREIRKEAAYFLQQLC 780

Query: 941  QSSSLTLQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRIS 1000
            QSS LTLQMF+ACRGIPVLV FLEADYAKYREMVHLAIDGMWQ+FKL+RST RN FCRI+
Sbjct: 781  QSSPLTLQMFIACRGIPVLVGFLEADYAKYREMVHLAIDGMWQVFKLKRSTPRNDFCRIA 840

Query: 1001 AKSGILLRLINTLYSLNEATRLASITVGAGNPFDG-FQRSRSGQLDSSHCIFSQYE-ASF 1060
            AK+GILLRLINTLYSLNEATRLASI+ G     DG   R RSGQLD ++ IF Q E +S 
Sbjct: 841  AKNGILLRLINTLYSLNEATRLASISGG----LDGQAPRVRSGQLDPNNPIFGQNETSSL 900

Query: 1061 PVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNAT 1120
             + DQPD+LK RHG        G  EPS AS+S+SQRSD +Q D  H   D  R+SS A 
Sbjct: 901  SMIDQPDVLKTRHG--------GGEEPSHASTSNSQRSDVHQPDALHPDGDKPRVSSVA- 960

Query: 1121 NEALGTSKPPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFL 1180
                                     P    S   +   + RI   +++NRTSTD+  K  
Sbjct: 961  -------------------------PDASTSGTEDVRQQHRIS--LSANRTSTDKLQKLA 1020

Query: 1181 EPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHA 1240
            E ASNGFP    VT  EQVRPLLSLLDKEPPSRH SGQL+Y+ H++G+ERHE+ +PLLH 
Sbjct: 1021 EGASNGFP----VTQTEQVRPLLSLLDKEPPSRHYSGQLDYVKHITGIERHESRLPLLHG 1080

Query: 1241 SNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGI 1300
            SNEKK NG+LDFLMAEFAEVS RGKENG+LD T++   KT+ KKV   ++ EG  STSGI
Sbjct: 1081 SNEKKNNGDLDFLMAEFAEVSGRGKENGSLDTTTRYPSKTMTKKV---LAIEGVASTSGI 1140

Query: 1301 ASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTT 1360
            ASQTASGVLSGSGVLNA PGS+TSSGLL+HMVST++ADVAREYL KV+DLLLEFA+ADTT
Sbjct: 1141 ASQTASGVLSGSGVLNARPGSATSSGLLAHMVSTLSADVAREYLEKVADLLLEFARADTT 1200

Query: 1361 VKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLE 1420
            VKSYMCS SLL+RLFQMFNRVEP ILLKIL+C NHLSTDPNCLENLQRADAIK+LIPNLE
Sbjct: 1201 VKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHLSTDPNCLENLQRADAIKHLIPNLE 1260

Query: 1421 LKEGSLVSQIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLC 1480
            LK+G LV QIH EVLS LFNLCKINKRRQE A ENGIIPHLM FIMSDSPLK+ ALPLLC
Sbjct: 1261 LKDGHLVYQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLMLFIMSDSPLKQYALPLLC 1320

Query: 1481 DMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKE 1540
            DMAHASRNSREQLRAHGGLDVYLSLL+DE WSV ALDSIAVCLA DNDNRKVEQALLK++
Sbjct: 1321 DMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIALDSIAVCLAQDNDNRKVEQALLKQD 1368

Query: 1541 AIKKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARL 1600
            AI+KLV FFQ CPE+HFVHILEPFLKIITKS RIN TLAVNGLTPLLI+RLDHQDAIARL
Sbjct: 1381 AIQKLVDFFQSCPERHFVHILEPFLKIITKSYRINKTLAVNGLTPLLISRLDHQDAIARL 1368

Query: 1601 NLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALH 1613
            NLLKLIKAVYEHHPRPKQLIVENDLP KLQNLIEERRDGQRSGGQVLVKQMATSLLKALH
Sbjct: 1441 NLLKLIKAVYEHHPRPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKALH 1368

BLAST of Cp4.1LG02g15870 vs. ExPASy Swiss-Prot
Match: Q9SFB6 (MAP3K epsilon protein kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MAP3KE2 PE=2 SV=1)

HSP 1 Score: 1622.1 bits (4199), Expect = 0.0e+00
Identity = 946/1507 (62.77%), Postives = 1073/1507 (71.20%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            M+RQ+  + FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIGQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LN IM                       QEIDLLKNLNHKNIVKYLGSLKTK+HLHIILE
Sbjct: 61   LNTIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKTHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS ANIIKPNKFGPFPESLV +YI+QVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSLANIIKPNKFGPFPESLVTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKL EAD NTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLNEADFNTHSVVGTPYWMAPEVIELSGVCAASDIWSVGCTIIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D  PPIPDSLSPDITDFLR CFKKD+RQRPDAKTLLSHPW++N RRAL S
Sbjct: 301  PMPALYRIVQDDTPPIPDSLSPDITDFLRLCFKKDSRQRPDAKTLLSHPWIRNSRRALRS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGT+R  +   S   + + G  Q   ES SAEK E+     K +S+ +L       
Sbjct: 361  SLRHSGTIRYMKETDSSSEKDAEG-SQEVVESVSAEKVEVT----KTNSKSKLPVIGGAS 420

Query: 581  TSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLA------TSEPTE 640
                +   +     EEG   E+D   DQ PTLS+ D  S  +G+  ++      + +  E
Sbjct: 421  FRSEKDQSSPSDLGEEGTDSEDDINSDQGPTLSMHDKSSRQSGTCSISSDAKGTSQDVLE 480

Query: 641  SHESHGRAHDEVIMNGGVPSAE-LRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKV 700
            +HE + R  DE+  N    ++E  R   + K   KE S  S +S   F  +G D   +K 
Sbjct: 481  NHEKYDR--DEIPGNLETEASEGRRNTLATKLVGKEYSIQSSHS---FSQKGEDG-LRKA 540

Query: 701  SKMSIASGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNN 760
             K   + GGNEL++FSD PGDASL DLF PLDK+P  ++ EASTS  T   N G+ PV +
Sbjct: 541  VKTPSSFGGNELTRFSDPPGDASLHDLFHPLDKVPEGKTNEASTSTPTANVNQGDSPVAD 600

Query: 761  VGKNDLATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDV-DIDGLVFDEKLPGEN 820
             GKNDLATKLRA IAQKQME E G    G DL RL+MGVL DDV +ID LVFDEK+P EN
Sbjct: 601  GGKNDLATKLRARIAQKQMEGETGHSQDGGDLFRLMMGVLKDDVLNIDDLVFDEKVPPEN 660

Query: 821  LYPLPAVEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLE 880
            L+PL AVEF RLV SLRPDE ED IV++  KL+A+F  RP QK V+VTQ+G LPL +LL+
Sbjct: 661  LFPLQAVEFSRLVSSLRPDESEDAIVTSSLKLVAMFRQRPGQKAVFVTQNGFLPLMDLLD 720

Query: 881  VPKTRITCSVLQLINQIVKDNDDFQENACLVGLIPLVMGFA--VPDRPREVRMEAAYFFQ 940
            +PK+R+ C+VLQLIN+IVKDN DF ENACLVGLIPLVM FA    DR RE+R EAAYF Q
Sbjct: 721  IPKSRVICAVLQLINEIVKDNTDFLENACLVGLIPLVMSFAGFERDRSREIRKEAAYFLQ 780

Query: 941  QLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFC 1000
            QLCQSS LTLQMF++CRGIPVLV FLEADYAK+REMVHLAIDGMWQ+FKL++ST RN FC
Sbjct: 781  QLCQSSPLTLQMFISCRGIPVLVGFLEADYAKHREMVHLAIDGMWQVFKLKKSTSRNDFC 840

Query: 1001 RISAKSGILLRLINTLYSLNEATRLASITVGAGNPFDG-FQRSRSGQLDSSHCIFSQYEA 1060
            RI+AK+GILLRL+NTLYSL+EATRLASI+ G     DG   R+RSGQLD ++ IFSQ E 
Sbjct: 841  RIAAKNGILLRLVNTLYSLSEATRLASIS-GDALILDGQTPRARSGQLDPNNPIFSQRET 900

Query: 1061 SFPVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSN 1120
            S  V D PD LK R+G        G  EPS A +S+SQ SD +Q D  H   D  RLSS 
Sbjct: 901  SPSVIDHPDGLKTRNG--------GGEEPSHALTSNSQSSDVHQPDALHPDGDRPRLSS- 960

Query: 1121 ATNEALGTSKPPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPK 1180
                               VV  AT++    V ++H      RI   +++NRTSTD+  K
Sbjct: 961  -------------------VVADATED----VIQQH------RIS--LSANRTSTDKLQK 1020

Query: 1181 FLEPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLL 1240
              E ASNGFP    VT  +QVRPLLSLL+KEPPSR +SGQL+Y+ H++G+ERHE+ +PLL
Sbjct: 1021 LAEGASNGFP----VTQPDQVRPLLSLLEKEPPSRKISGQLDYVKHIAGIERHESRLPLL 1080

Query: 1241 HASNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTS 1300
            +AS+EKK NG+L+F+MAEFAEVS RGKENGNLD   + S KT+ KKV   ++ E   ST 
Sbjct: 1081 YASDEKKTNGDLEFIMAEFAEVSGRGKENGNLDTAPRYSSKTMTKKV---MAIERVASTC 1140

Query: 1301 GIASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQAD 1360
            GIASQTASGVLSGSGVLNA PGS+TSSGLL+H +S   ADV+ +YL KV+DLLLEFA+A+
Sbjct: 1141 GIASQTASGVLSGSGVLNARPGSTTSSGLLAHALS---ADVSMDYLEKVADLLLEFARAE 1200

Query: 1361 TTVKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPN 1420
            TTVKSYMCS SLL+RLFQMFNRVEP ILLKIL+C NHLSTDPNCLENLQRADAIK LIPN
Sbjct: 1201 TTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHLSTDPNCLENLQRADAIKQLIPN 1260

Query: 1421 LELKEGSLVSQIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPL 1480
            LELKEG LV QIH EVLS LFNLCKINKRRQE A ENGIIPHLM F+MSDSPLK+ ALPL
Sbjct: 1261 LELKEGPLVYQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLMLFVMSDSPLKQYALPL 1320

Query: 1481 LCDMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLK 1540
            LCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV ALDSIAVCLA D D +KVEQA LK
Sbjct: 1321 LCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIALDSIAVCLAQDVD-QKVEQAFLK 1367

Query: 1541 KEAIKKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIA 1600
            K+AI+KLV FFQ CPE+HFVHILEPFLKIITKSS IN TLA+NGLTPLLIARLDHQDAIA
Sbjct: 1381 KDAIQKLVNFFQNCPERHFVHILEPFLKIITKSSSINKTLALNGLTPLLIARLDHQDAIA 1367

Query: 1601 RLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKA 1613
            RLNLLKLIKAVYE HP+PKQLIVENDLP KLQNLIEERRDGQRSGGQVLVKQMATSLLKA
Sbjct: 1441 RLNLLKLIKAVYEKHPKPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKA 1367

BLAST of Cp4.1LG02g15870 vs. ExPASy Swiss-Prot
Match: A0A078CGE6 (MAP3K epsilon protein kinase 1 OS=Brassica napus OX=3708 GN=M3KE1 PE=1 SV=1)

HSP 1 Score: 1563.5 bits (4047), Expect = 0.0e+00
Identity = 918/1500 (61.20%), Postives = 1031/1500 (68.73%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            M+RQ+  + FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIVQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LN IM                       QEIDLLKNLNHKNIVKYLGSLKTK+HLHIILE
Sbjct: 61   LNTIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKTHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS ANIIKPNKFGPFPESLV +YI+QVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSLANIIKPNKFGPFPESLVTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKL EADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLNEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D  PPIPDSLSPDITDFLRQCFKKD+RQRPDAKTLLSHPW++N RRAL S
Sbjct: 301  PMPALFRIVQDDSPPIPDSLSPDITDFLRQCFKKDSRQRPDAKTLLSHPWIRNSRRALQS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGD-DQNSCESPSAEKNEIAGSDFKADSRKELASDVAT 580
            SLRHSGT+R   + G+  +   +G+  Q+  ES SAEK  ++    K +S+ +L   V +
Sbjct: 361  SLRHSGTIR--YMKGADSSSEKDGEGSQDIAESVSAEKVGMS----KTNSKSKLG--VGS 420

Query: 581  DTSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
              S+  +  AS    EE    E+D   DQ PTLSI DN S L  S   + S   +     
Sbjct: 421  FRSEKDQSSAS-DIGEERADSEDDIMSDQGPTLSIHDNKSSLQ-SSTCSISSDAKGTSQD 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            G++  +  +     S   RK ++ K   KE+S      S  FGP+G D   +K  K   +
Sbjct: 481  GKSEPDGNLEMEA-SEGRRKASATKQVGKESSIQMQQRSHSFGPKGEDRGLRKAVKTPSS 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
             GGNEL++FSD PGDA L DLF PL+K+P  +  EAS S     +N G+ PV + GKNDL
Sbjct: 541  YGGNELTRFSDPPGDACLHDLFHPLNKVPEGKLNEASASTPASNANQGDSPVADGGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDV-DIDGLVFDEKLPGENLYPLPA 820
            ATKLRA IAQKQME E G  N G DL RL+MGVL DDV DIDGLVFDEK   +NL PL A
Sbjct: 601  ATKLRARIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEKASPDNLLPLQA 660

Query: 821  VEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRI 880
            VEF RLV SLRP E ED IV++C KL+A+F HRPEQK+V+VTQHG LP+ +LL+ PK+R+
Sbjct: 661  VEFSRLVSSLRPSETEDAIVTSCQKLVAMFRHRPEQKVVFVTQHGFLPVMDLLDSPKSRV 720

Query: 881  TCSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVP--DRPREVRMEAAYFFQQLCQSS 940
            TC+VLQLIN+I+KDN DFQENACLVGLIPLVM FA P  DR RE+R EAAYF QQLCQSS
Sbjct: 721  TCAVLQLINEIIKDNIDFQENACLVGLIPLVMSFAGPERDRSREIRKEAAYFLQQLCQSS 780

Query: 941  SLTLQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKS 1000
            SLTLQMF+ACRGIPVLV FLEADYAKYR MVHLAIDGMWQ+FKL+RST RN FCRI+AK+
Sbjct: 781  SLTLQMFIACRGIPVLVGFLEADYAKYRSMVHLAIDGMWQVFKLKRSTPRNDFCRIAAKN 840

Query: 1001 GILLRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQ 1060
            GILLRLINTLYSLNEAT LAS               RSGQLD       Q+EA   V D 
Sbjct: 841  GILLRLINTLYSLNEATLLAS-------------EGRSGQLD-------QHEALLSVIDH 900

Query: 1061 PDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALG 1120
            PD+LK R G        G  EP     S+SQRSD  Q D      D  R SS A +    
Sbjct: 901  PDVLKTRPG--------GGEEP-----SNSQRSDLYQPD-----GDRPRSSSAALD---- 960

Query: 1121 TSKPPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASN 1180
                            AT++      K+H  +        ++SNRTSTD+  K  E ASN
Sbjct: 961  ----------------ATED-----VKQHHRIS-------ISSNRTSTDKIQKLAESASN 1020

Query: 1181 GFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKK 1240
            G+     VT  EQVRPLLSLL+KEPPSRH+SGQL+Y+ H++GLE+HE+I+PLL AS    
Sbjct: 1021 GY----AVTQPEQVRPLLSLLEKEPPSRHVSGQLDYVKHIAGLEKHESILPLLRAS---- 1080

Query: 1241 MNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTA 1300
                                    +D   +   KT+ KKV   ++ EGA S        A
Sbjct: 1081 ------------------------IDTMPRYFSKTMSKKV---MAIEGAAS--------A 1140

Query: 1301 SGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYM 1360
            SGVLSGSGVLNA  GS TSSGLLSHMV+T++A+VA +YL KV+DLLLEFA+ADTTVKSYM
Sbjct: 1141 SGVLSGSGVLNARLGSDTSSGLLSHMVTTLSAEVASQYLEKVADLLLEFARADTTVKSYM 1200

Query: 1361 CSHSLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGS 1420
            CS SLL+RLF MFNRVEP ILLKILKC NHLSTDPNCLE+LQRADAIK+LIPNLE+KEG+
Sbjct: 1201 CSQSLLSRLFHMFNRVEPPILLKILKCTNHLSTDPNCLESLQRADAIKHLIPNLEVKEGN 1260

Query: 1421 LVSQIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHA 1480
            LV QIH EVLS LFNLCKINKRRQE A ENGIIPHLM F+MSDSPLK+ ALPLLCDMAHA
Sbjct: 1261 LVDQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLMLFVMSDSPLKQYALPLLCDMAHA 1299

Query: 1481 SRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKL 1540
            SRNSREQLR+HGGLDVYLSLL+DE WSV ALDSIAVCLA DNDNRKVEQALLK +AI  L
Sbjct: 1321 SRNSREQLRSHGGLDVYLSLLDDEYWSVIALDSIAVCLAQDNDNRKVEQALLKDDAIYTL 1299

Query: 1541 VKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKL 1600
            V FFQ CPE+HFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKL
Sbjct: 1381 VNFFQSCPERHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKL 1299

Query: 1601 IKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1613
            IKAVYEHHPRPKQLIVENDLP +LQNLIEERR+GQ  GGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 IKAVYEHHPRPKQLIVENDLPQRLQNLIEERREGQHLGGQVLVKQMATSLLKALHINTVL 1299

BLAST of Cp4.1LG02g15870 vs. ExPASy Swiss-Prot
Match: Q8T2I8 (Serine/threonine-protein kinase sepA OS=Dictyostelium discoideum OX=44689 GN=sepA PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 9.6e-130
Identity = 432/1586 (27.24%), Postives = 633/1586 (39.91%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MS++ P     K+ T+ N Y LG  IGKG +G VY+GLD+E+GDFVAIKQ++L  I ++ 
Sbjct: 1    MSKKEP-EEIKKNVTVGN-YNLGVVIGKGGFGTVYQGLDIEDGDFVAIKQINLTKIPKDQ 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            L  IM                        EIDLLKNLNH NIVKY+  +KTK +L+I+LE
Sbjct: 61   LQGIM-----------------------NEIDLLKNLNHANIVKYIKYVKTKDNLYIVLE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS + IIK  KFG FPE+LV +YI QVLEGLVYLHEQGV+HRDIKGANILTTKE  
Sbjct: 121  YVENGSLSGIIK--KFGKFPETLVCVYIRQVLEGLVYLHEQGVVHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                G +KLADF
Sbjct: 181  ----------------------------------------------------GKIKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATK    D +  +VVGTPYWMAPE                                  
Sbjct: 241  GVATKFD--DTSAAAVVGTPYWMAPEIIELNGATTKSDIWSVGCTVIELLTGSPPYYDLG 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D  PP+P+ +SP + D+L QCF+KD   R  A+ LL H W+Q   +    
Sbjct: 301  QMPALFRIVQDDCPPLPEGISPPLKDWLMQCFQKDPNLRISAQKLLKHKWIQASIKKKPV 360

Query: 521  SLRHSGTLRNTQLDGSPGAEI----SNGDDQNS--------CESPSAEKNEIAGSDF--K 580
                 G    T   G+P A I     N  D N            PS       G  F   
Sbjct: 361  ENGAGGVGNGTDSLGAP-ANIDDIAKNITDYNERINKKPSHQRKPSIHPKSPKGKVFLPP 420

Query: 581  ADSRKELASDVATDTSKSQKHFASGHFDEEGEGIEEDTPLDQVPT--------------- 640
             +  ++   D  ++T KS K         +    +  TPL Q PT               
Sbjct: 421  PEEEEDEWGDDFSNTPKSIK-LPDKKSPLKLTNNKPSTPLKQQPTNNTPVQQQQQQQQPP 480

Query: 641  ---------------------LSIRDNLSLLTGSGRLATS-----------EPTESHESH 700
                                  +   +LS   GS     +           +PT S +  
Sbjct: 481  PLKLAVPKQPVIENDDDWGDDFNTVSDLSKAVGSLNFNNNKKNETPKPNIKKPTFSEDED 540

Query: 701  GRAHDEVIMNGG---------VPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCF 760
                D+   +GG         +P+       S K   K  S   GNSS         +  
Sbjct: 541  EDDDDDGFGSGGDDEDDDFGDIPT-------SIKLNPKFGSNIKGNSSGSANTTNSSSTV 600

Query: 761  QKVSKMSIASGGNELS-KFSDTPGDASLDDLFQP--------------LDKLPADRSAEA 820
             +  K+++++  N  + K   +P   S  ++ +               +D+   D   + 
Sbjct: 601  VQQPKLTVSNNNNNNNKKLPLSPRQPSSGNVKEGINHGSTGSKSGGVIIDQWGEDGEEDN 660

Query: 821  S-TSLSTPQSNIGNVPVNNVGKNDLATKL--RATIAQKQMENEMGQVNGGDDLLRLVMGV 880
                ++T   +   +    V K DL+T+L  R  +++  + N     NG DD    +   
Sbjct: 661  DWGDVATVNFDPKVIRKGTVNKPDLSTRLKNRIALSETALSNSFNN-NGNDDEDEDIFAD 720

Query: 881  LNDDVDIDGLVFDEKLPGENLYPLPAVEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRP 940
              D+ D +    D+ L  +N Y   + E  +L+  L P++PE+VI SAC +LI +F    
Sbjct: 721  DFDEDDDEDFDLDKNLMKDN-YARMSSEILKLMNLLTPEQPEEVISSACTQLITMFKENS 780

Query: 941  EQKIVYVTQHGLLPLTELLEVP--KTRITCSVLQLINQIVKDNDDFQENACLVGLIPLVM 1000
            EQK + + +HG++P+ E+LEV   ++ + CS+L+++NQI+ +N + QEN CLVG IP +M
Sbjct: 781  EQKTLLIRRHGVIPIMEMLEVSNIQSHVLCSILKVVNQIIDNNMEIQENLCLVGGIPAIM 840

Query: 1001 GFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYREMVHLA 1060
             F+ P+ P  VR+E A F  ++C +S+LTLQMF+AC+G+P+LV FL + YA+ + +V +A
Sbjct: 841  KFSGPEYPASVRLETASFISKMCSTSTLTLQMFIACKGLPILVDFLLSPYAESKRLVWMA 900

Query: 1061 IDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASITVGAGNPFDGFQ 1120
            +D +  +F+LQ  T +N FCR+ +K G+L                               
Sbjct: 901  VDAIVNVFELQSPTPKNDFCRLFSKCGLL------------------------------- 960

Query: 1121 RSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSD 1180
                                                                        
Sbjct: 961  ------------------------------------------------------------ 1020

Query: 1181 ANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSGFVSKEHENVDR 1240
                                                                        
Sbjct: 1021 ------------------------------------------------------------ 1080

Query: 1241 WRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQL 1300
                                                                        
Sbjct: 1081 ------------------------------------------------------------ 1140

Query: 1301 EYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLK 1360
                                                                        
Sbjct: 1141 ------------------------------------------------------------ 1167

Query: 1361 TVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADV 1420
                K  P+V  +                                        S  + + 
Sbjct: 1201 ----KTLPIVLRD----------------------------------------SIADGEA 1167

Query: 1421 AREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHLSTD 1480
            A  Y  ++ +L + F+ AD+ V+  M +  ++  +    +++ P  L K+LK I  LS D
Sbjct: 1261 AATYPDRIINLFIMFSAADSVVRKTMSAVEVIRPILDTLSQLMPEQLAKVLKSIKQLSMD 1167

Query: 1481 PNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRRQECAIENGIIP 1540
             N L NLQ A AI++++P L  + G+ V++IH  VL+T+F+LC+I+  RQ  A  +GIIP
Sbjct: 1321 HNTLANLQNAGAIRFMVPFLGRRTGAFVAEIHNHVLNTMFHLCRIDPERQYQAAIDGIIP 1167

Query: 1541 HLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSI 1600
            HL +FI S SPL + ALP++CD+AH S+ +R +L  + G+  YLSLLE+  W V ALDS+
Sbjct: 1381 HLQYFITSHSPLNQFALPIICDLAH-SKKARSELWKNNGVAFYLSLLEERYWQVNALDSL 1167

Query: 1601 AVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLA 1613
            AV +   ++  KVE  +   E IKKL++ F     Q F  ILEP LKII  S  +N  L 
Sbjct: 1441 AVWIT--DETHKVENIIATNENIKKLIQLFTNAESQSFAGILEPLLKIIQISIPVNILLG 1167

BLAST of Cp4.1LG02g15870 vs. ExPASy Swiss-Prot
Match: A2QHV0 (Cytokinesis protein sepH OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=sepH PE=3 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 2.3e-99
Identity = 403/1588 (25.38%), Postives = 603/1588 (37.97%), Query Frame = 0

Query: 151  SSPAEADSATMSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQ 210
            SSPA+ D     R V  +A   ++  D  Y LGD +GKGA+G VY+ L+   G+ VA+KQ
Sbjct: 32   SSPAKQDKPKDDRVVKSSAKDVAELKD--YQLGDCLGKGAFGSVYRALNWNTGETVAVKQ 91

Query: 211  VSLENIAQEDLNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLK 270
            + L ++ + +L VIM                        EIDLLKNL+H NIVKY G +K
Sbjct: 92   IKLVDLPKSELRVIML-----------------------EIDLLKNLDHPNIVKYQGFVK 151

Query: 271  TKSHLHIILEYVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKG 330
            +   L+IILEY ENGS  +I K   FG FPE+LV LY+SQVL GL+YLHEQGVIHRDIKG
Sbjct: 152  SAETLNIILEYCENGSLHSIAK--NFGRFPENLVGLYMSQVLHGLLYLHEQGVIHRDIKG 211

Query: 331  ANILTTKEARHTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLG 390
            ANILTTKE                                                    
Sbjct: 212  ANILTTKE---------------------------------------------------- 271

Query: 391  LGGLVKLADFGVATKLTEADVNTHSVVGTPYWMAPE------------------------ 450
              GLVKLADFGVA++ T   ++  SVVGTPYWMAPE                        
Sbjct: 272  --GLVKLADFGVASRTT--GLSESSVVGTPYWMAPEVIELSGATTASDIWSLGCTVIELL 331

Query: 451  --------------------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPW 510
                                D HPP+P   SP + DFL QCF+KD   R  A+ LL HPW
Sbjct: 332  EGKPPYYNLQPMPALFRIVNDDHPPLPQGASPAVKDFLMQCFQKDPNLRVSARKLLKHPW 391

Query: 511  VQNCRRALHSSLRHSGTLRNTQLDGSPGAEISNGDDQNSCESP-------SAEKNEIAGS 570
            + N RR+     + S              E     D  +   P       +A + E+A S
Sbjct: 392  IVNARRSDSVVPKKSTEYEEAVKSVQEWNEALRSPDTGTLRKPYRYDAQGAALRPEMAPS 451

Query: 571  DF--KADSRKELASDVATDTSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLL 630
             +    D      S   TD  +S       ++D++       + L Q+P L  +DN    
Sbjct: 452  RYTPTKDILPSPVSKHVTDRFRSPDSTEEDNWDDDFATAISPSAL-QLPHLRPQDNF--- 511

Query: 631  TGSGRLATSEPTESHESHGRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGF 690
               G + +SE  ++  S     D  ++       +     S++ GE +   T     +  
Sbjct: 512  ---GGMLSSEKLKAFAS----LDGTVLKSEDGFDDFDDPFSQQPGESDPLRTI--RPYSA 571

Query: 691  GPEGHDNCFQKVSKMSIAS------------------------GGNELSKFSD--TPGDA 750
             P G +N  Q+ +K +IA+                          N +  +SD  +  + 
Sbjct: 572  KPTGMENMSQQ-TKPTIAAMHHNVPVLKTPVPPLRPQRPTSYFKENSVEDYSDLISANED 631

Query: 751  SLDD---LFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLATKLRATIA---- 810
             LDD    FQ +D+  +D       S+ +P   +     +     D   +LR  I+    
Sbjct: 632  ILDDKLSAFQDIDEEGSD------ISIPSPSKEVVRYQASPDHDEDHQPQLRKRISVKRH 691

Query: 811  ------QKQMENEMGQVNG---GDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLY----- 870
                  QK  ENE  +      G D + L     ++  D   L+ + KL   +       
Sbjct: 692  RSAIEIQKFAENERDEDFSDILGADEVALDKPESDEGSDRSTLMLNSKLSNNSWLGDQDD 751

Query: 871  ---PLPAVEFG-----------------------RLVGSLRPDEPEDVIVSACHKLIAIF 930
               P   +E G                        LV SL+  + EDV+     +L+ +F
Sbjct: 752  EDDPFAQLEEGLDEVDLEANIARDKYARLRGQVEGLVSSLKTSQDEDVLGEISEQLLTVF 811

Query: 931  HHRPEQKIVYVTQHGLLPLTELLEVPKTR-ITCSVLQLINQIVKDNDDFQENACLVGLIP 990
               PE K + ++ HG+LP+ E+L++ + R I   +L+++N I+ ++ + QEN C VG IP
Sbjct: 812  CDLPETKNIIISAHGMLPILEILDICRRRDIILCLLRIVNAIIFNDYEIQENLCFVGGIP 871

Query: 991  LVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYREMV 1050
            ++  FA    PRE+R+EAA F QQ+ Q+S+LTLQMFV+  G+ VLV FLE DY   R++V
Sbjct: 872  IINEFASKKYPREIRLEAAAFVQQMYQTSTLTLQMFVSAGGLNVLVEFLEDDYEDERDLV 931

Query: 1051 HLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASITVGAGNPFD 1110
             + ++G+W +F LQ ST +N FCRI +                                 
Sbjct: 932  LIGVNGIWSVFDLQGSTPKNDFCRILS--------------------------------- 991

Query: 1111 GFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQ 1170
                                               R+ ++DP                  
Sbjct: 992  -----------------------------------RNSVLDP------------------ 1051

Query: 1171 RSDANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSGFVSKEHEN 1230
                                                                        
Sbjct: 1052 ------------------------------------------------------------ 1111

Query: 1231 VDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLS 1290
                                              S+V        L  +LD+E       
Sbjct: 1112 ---------------------------------LSLV--------LSRVLDEE------- 1171

Query: 1291 GQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKV 1350
                                           GEL    AE  E                 
Sbjct: 1172 -------------------------------GEL----AEIVE----------------- 1192

Query: 1351 SLKTVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMN 1410
                                                                        
Sbjct: 1232 ------------------------------------------------------------ 1192

Query: 1411 ADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHL 1470
                     +++++   F+QA+  VK  +   ++L+R+ +   R+ P+  + +LK I +L
Sbjct: 1292 --------GRIANIFFIFSQAENHVKEMVAERTVLHRVLKELKRMTPAHQITMLKFIKNL 1192

Query: 1471 STDPNCLENLQRADAIKYLIPNLELK-EGSLVSQIHTEVLSTLFNLCKINKRRQECAIEN 1530
            S     L++LQ ++AI  L   L    +     ++  ++L+T++N+C++NK RQE A  N
Sbjct: 1352 SMLSTTLDSLQNSNAIDVLTDLLRSTIKRPHFREVSNQILNTIYNMCRLNKSRQEDAALN 1192

Query: 1531 GIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTA 1590
            GI+P L   + ++ PLK+ ALP+LCDMAH+ +  R +L  + GL  Y+SLL D  W VTA
Sbjct: 1412 GIVPLLQKIVKTERPLKEFALPILCDMAHSGKVGRRELWRNKGLAFYISLLSDPYWQVTA 1192

Query: 1591 LDSIAVCLAHDNDNRKVEQALL-----KKEAIKKLVKFFQCCPEQHFVHILEPFLKIITK 1606
            LD+I   L    +  KVE+ LL     K      +V+         F +ILEP  K++  
Sbjct: 1472 LDAIFTWL--QEETAKVEEHLLDNRYDKMSFTDSIVRCLTISKANAFENILEPLQKLLRL 1192

BLAST of Cp4.1LG02g15870 vs. NCBI nr
Match: XP_023525640.1 (MAP3K epsilon protein kinase 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023525641.1 MAP3K epsilon protein kinase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2625 bits (6803), Expect = 0.0
Identity = 1375/1496 (91.91%), Postives = 1375/1496 (91.91%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 420

Query: 581  TSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESHG 640
            TSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESHG
Sbjct: 421  TSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESHG 480

Query: 641  RAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIAS 700
            RAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIAS
Sbjct: 481  RAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIAS 540

Query: 701  GGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLA 760
            GGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLA
Sbjct: 541  GGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLA 600

Query: 761  TKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVE 820
            TKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVE
Sbjct: 601  TKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVE 660

Query: 821  FGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITC 880
            FGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITC
Sbjct: 661  FGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITC 720

Query: 881  SVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTL 940
            SVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTL
Sbjct: 721  SVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTL 780

Query: 941  QMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILL 1000
            QMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILL
Sbjct: 781  QMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILL 840

Query: 1001 RLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLL 1060
            RLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLL
Sbjct: 841  RLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLL 900

Query: 1061 KVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKP 1120
            KVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKP
Sbjct: 901  KVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKP 960

Query: 1121 PELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPT 1180
            PELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPT
Sbjct: 961  PELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPT 1020

Query: 1181 ASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGE 1240
            ASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGE
Sbjct: 1021 ASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGE 1080

Query: 1241 LDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVL 1300
            LDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVL
Sbjct: 1081 LDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVL 1140

Query: 1301 SGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHS 1360
            SGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHS
Sbjct: 1141 SGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHS 1200

Query: 1361 LLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQ 1420
            LLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQ
Sbjct: 1201 LLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQ 1260

Query: 1421 IHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNS 1480
            IHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNS
Sbjct: 1261 IHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNS 1320

Query: 1481 REQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFF 1540
            REQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFF
Sbjct: 1321 REQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFF 1380

Query: 1541 QCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAV 1600
            QCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAV
Sbjct: 1381 QCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAV 1419

Query: 1601 YEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            YEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 YEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1419

BLAST of Cp4.1LG02g15870 vs. NCBI nr
Match: KAG7036612.1 (MAP3K epsilon protein kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2593 bits (6721), Expect = 0.0
Identity = 1363/1497 (91.05%), Postives = 1366/1497 (91.25%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPG EISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGEEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +FDEEGE IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFDEEGEAIEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRS+EASTSLSTPQSNIGNVPVNNVGKNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSSEASTSLSTPQSNIGNVPVNNVGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEA FPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEAPFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLSAGTPEPSRASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. NCBI nr
Match: XP_022949075.1 (MAP3K epsilon protein kinase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2592 bits (6718), Expect = 0.0
Identity = 1361/1497 (90.92%), Postives = 1367/1497 (91.32%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPG EISNGDDQNSCESPSAEKNEIAGSDFKADSRK+LASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGEEISNGDDQNSCESPSAEKNEIAGSDFKADSRKDLASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +FDEEGE IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFDEEGEAIEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRS+EASTSLSTPQSNIGNVPVNNVGKNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSSEASTSLSTPQSNIGNVPVNNVGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEK+PGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKMPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLSAGTPEPSRASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVG+KVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGRKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. NCBI nr
Match: KAG6606908.1 (MAP3K epsilon protein kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2588 bits (6709), Expect = 0.0
Identity = 1361/1497 (90.92%), Postives = 1364/1497 (91.12%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPG EISNGDDQNSCESPS EKNEIAGSDFKADSRKELASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGEEISNGDDQNSCESPSTEKNEIAGSDFKADSRKELASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +FDEEGE IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFDEEGEAIEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRS+EASTSLSTPQSNIGNV VNNVGKNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSSEASTSLSTPQSNIGNVAVNNVGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEA FPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEAPFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLSAGTPEPSRASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. NCBI nr
Match: XP_022998374.1 (MAP3K epsilon protein kinase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 2584 bits (6697), Expect = 0.0
Identity = 1361/1497 (90.92%), Postives = 1363/1497 (91.05%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKE ASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKEFASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +F EEGE IEEDT LDQVPTLSIRDNLSLL GSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFVEEGEAIEEDTLLDQVPTLSIRDNLSLLNGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNV KNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVEKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSH IFSQYEASFPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHSIFSQYEASFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLS GTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSTGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMV PQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVMPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. ExPASy TrEMBL
Match: A0A6J1GBR9 (MAP3K epsilon protein kinase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452538 PE=4 SV=1)

HSP 1 Score: 2592 bits (6718), Expect = 0.0
Identity = 1361/1497 (90.92%), Postives = 1367/1497 (91.32%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPG EISNGDDQNSCESPSAEKNEIAGSDFKADSRK+LASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGEEISNGDDQNSCESPSAEKNEIAGSDFKADSRKDLASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +FDEEGE IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFDEEGEAIEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRS+EASTSLSTPQSNIGNVPVNNVGKNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSSEASTSLSTPQSNIGNVPVNNVGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEK+PGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKMPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLSAGTPEPSRASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVG+KVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGRKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. ExPASy TrEMBL
Match: A0A6J1KE60 (MAP3K epsilon protein kinase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493031 PE=4 SV=1)

HSP 1 Score: 2584 bits (6697), Expect = 0.0
Identity = 1361/1497 (90.92%), Postives = 1363/1497 (91.05%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKE ASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKEFASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +F EEGE IEEDT LDQVPTLSIRDNLSLL GSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFVEEGEAIEEDTLLDQVPTLSIRDNLSLLNGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNV KNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVEKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
            CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSH IFSQYEASFPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHSIFSQYEASFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLS GTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSTGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMV PQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVMPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1420

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1420

BLAST of Cp4.1LG02g15870 vs. ExPASy TrEMBL
Match: A0A6J1GAZ4 (MAP3K epsilon protein kinase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452538 PE=4 SV=1)

HSP 1 Score: 2552 bits (6614), Expect = 0.0
Identity = 1341/1477 (90.79%), Postives = 1347/1477 (91.20%), Query Frame = 0

Query: 181  MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIMCSHWIFFPSLLLKLL 240
            MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIM               
Sbjct: 1    MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIM--------------- 60

Query: 241  VGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP 300
                    QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP
Sbjct: 61   --------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP 120

Query: 301  ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEARHTRTASLRLKSKEKIYFFEG 360
            ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE                      
Sbjct: 121  ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE---------------------- 180

Query: 361  CFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADFGVATKLTEADVNTHSVVGTP 420
                                            GLVKLADFGVATKLTEADVNTHSVVGTP
Sbjct: 181  --------------------------------GLVKLADFGVATKLTEADVNTHSVVGTP 240

Query: 421  YWMAPE--------------------------------------------DKHPPIPDSL 480
            YWMAPE                                            DKHPPIPDSL
Sbjct: 241  YWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQDKHPPIPDSL 300

Query: 481  SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPGAE 540
            SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPG E
Sbjct: 301  SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPGEE 360

Query: 541  ISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATDTSKSQKHFASG-HFDEEGEG 600
            ISNGDDQNSCESPSAEKNEIAGSDFKADSRK+LASDVATDTS SQKHFASG +FDEEGE 
Sbjct: 361  ISNGDDQNSCESPSAEKNEIAGSDFKADSRKDLASDVATDTSNSQKHFASGPNFDEEGEA 420

Query: 601  IEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESHGRAHDEVIMNGGVPSAELRK 660
            IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESHGRAHDEVIMNGGVPSAELRK
Sbjct: 421  IEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESHGRAHDEVIMNGGVPSAELRK 480

Query: 661  VASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIASGGNELSKFSDTPGDASLDD 720
            VASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIASGGNELSKFSDTPGDASLDD
Sbjct: 481  VASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIASGGNELSKFSDTPGDASLDD 540

Query: 721  LFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLATKLRATIAQKQMENEMGQV 780
            LFQPLDKLPADRS+EASTSLSTPQSNIGNVPVNNVGKNDLATKLRATIAQKQMENEMGQV
Sbjct: 541  LFQPLDKLPADRSSEASTSLSTPQSNIGNVPVNNVGKNDLATKLRATIAQKQMENEMGQV 600

Query: 781  NGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVEFGRLVGSLRPDEPEDVIVS 840
            NGGDDLLRLVMGVLNDDVDIDGLVFDEK+PGENLYPLPAVEFGRLVGSLRPDEPEDVIVS
Sbjct: 601  NGGDDLLRLVMGVLNDDVDIDGLVFDEKMPGENLYPLPAVEFGRLVGSLRPDEPEDVIVS 660

Query: 841  ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN 900
            ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN
Sbjct: 661  ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN 720

Query: 901  ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD 960
            ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD
Sbjct: 721  ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD 780

Query: 961  YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT 1020
            YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT
Sbjct: 781  YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT 840

Query: 1021 VGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVDPHLSAGTPEPS 1080
            VGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVD HLSAGTPEPS
Sbjct: 841  VGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVDHHLSAGTPEPS 900

Query: 1081 RASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG 1140
            RASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG
Sbjct: 901  RASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG 960

Query: 1141 FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDK 1200
            FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDK
Sbjct: 961  FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDK 1020

Query: 1201 EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG 1260
            EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG
Sbjct: 1021 EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG 1080

Query: 1261 NLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL 1320
            NLDPTSKVSLKTVG+KVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL
Sbjct: 1081 NLDPTSKVSLKTVGRKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL 1140

Query: 1321 SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK 1380
            SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK
Sbjct: 1141 SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK 1200

Query: 1381 ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR 1440
            ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR
Sbjct: 1201 ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR 1260

Query: 1441 QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED 1500
            QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED
Sbjct: 1261 QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED 1320

Query: 1501 EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII 1560
            EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII
Sbjct: 1321 EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII 1380

Query: 1561 TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK 1612
            TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK
Sbjct: 1381 TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK 1400

BLAST of Cp4.1LG02g15870 vs. ExPASy TrEMBL
Match: A0A6J1KCB6 (MAP3K epsilon protein kinase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493031 PE=4 SV=1)

HSP 1 Score: 2544 bits (6593), Expect = 0.0
Identity = 1341/1477 (90.79%), Postives = 1343/1477 (90.93%), Query Frame = 0

Query: 181  MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIMCSHWIFFPSLLLKLL 240
            MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIM               
Sbjct: 1    MLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIM--------------- 60

Query: 241  VGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP 300
                    QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP
Sbjct: 61   --------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFP 120

Query: 301  ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEARHTRTASLRLKSKEKIYFFEG 360
            ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE                      
Sbjct: 121  ESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE---------------------- 180

Query: 361  CFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADFGVATKLTEADVNTHSVVGTP 420
                                            GLVKLADFGVATKLTEADVNTHSVVGTP
Sbjct: 181  --------------------------------GLVKLADFGVATKLTEADVNTHSVVGTP 240

Query: 421  YWMAPE--------------------------------------------DKHPPIPDSL 480
            YWMAPE                                            DKHPPIPDSL
Sbjct: 241  YWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQPMPALFRIVQDKHPPIPDSL 300

Query: 481  SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPGAE 540
            SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPGAE
Sbjct: 301  SPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSSLRHSGTLRNTQLDGSPGAE 360

Query: 541  ISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATDTSKSQKHFASG-HFDEEGEG 600
            ISNGDDQNSCESPSAEKNEIAGSDFKADSRKE ASDVATDTS SQKHFASG +F EEGE 
Sbjct: 361  ISNGDDQNSCESPSAEKNEIAGSDFKADSRKEFASDVATDTSNSQKHFASGPNFVEEGEA 420

Query: 601  IEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESHGRAHDEVIMNGGVPSAELRK 660
            IEEDT LDQVPTLSIRDNLSLL GSGRLATSEPTE HESHGRAHDEVIMNGGVPSAELRK
Sbjct: 421  IEEDTLLDQVPTLSIRDNLSLLNGSGRLATSEPTEFHESHGRAHDEVIMNGGVPSAELRK 480

Query: 661  VASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIASGGNELSKFSDTPGDASLDD 720
            VASRK+GEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIASGGNELSKFSDTPGDASLDD
Sbjct: 481  VASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIASGGNELSKFSDTPGDASLDD 540

Query: 721  LFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDLATKLRATIAQKQMENEMGQV 780
            LFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNV KNDLATKLRATIAQKQMENEMGQV
Sbjct: 541  LFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVEKNDLATKLRATIAQKQMENEMGQV 600

Query: 781  NGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVEFGRLVGSLRPDEPEDVIVS 840
            NGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVEFGRLVGSLRPDEPEDVIVS
Sbjct: 601  NGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAVEFGRLVGSLRPDEPEDVIVS 660

Query: 841  ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN 900
            ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN
Sbjct: 661  ACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRITCSVLQLINQIVKDNDDFQEN 720

Query: 901  ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD 960
            ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD
Sbjct: 721  ACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLTLQMFVACRGIPVLVSFLEAD 780

Query: 961  YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT 1020
            YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT
Sbjct: 781  YAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGILLRLINTLYSLNEATRLASIT 840

Query: 1021 VGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDLLKVRHGIVDPHLSAGTPEPS 1080
            VGAGNPFDGFQRSRSGQLDSSH IFSQYEASFPVPDQPDLLKVRHGIVD HLS GTPEPS
Sbjct: 841  VGAGNPFDGFQRSRSGQLDSSHSIFSQYEASFPVPDQPDLLKVRHGIVDHHLSTGTPEPS 900

Query: 1081 RASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG 1140
            RASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG
Sbjct: 901  RASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSKPPELASLEKVVNLATKEPSG 960

Query: 1141 FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVTPQEQVRPLLSLLDK 1200
            FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMV PQEQVRPLLSLLDK
Sbjct: 961  FVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFPTASMVMPQEQVRPLLSLLDK 1020

Query: 1201 EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG 1260
            EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG
Sbjct: 1021 EPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNGELDFLMAEFAEVSQRGKENG 1080

Query: 1261 NLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL 1320
            NLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL
Sbjct: 1081 NLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGVLSGSGVLNAIPGSSTSSGLL 1140

Query: 1321 SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK 1380
            SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK
Sbjct: 1141 SHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSHSLLNRLFQMFNRVEPSILLK 1200

Query: 1381 ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR 1440
            ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR
Sbjct: 1201 ILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVSQIHTEVLSTLFNLCKINKRR 1260

Query: 1441 QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED 1500
            QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED
Sbjct: 1261 QECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRNSREQLRAHGGLDVYLSLLED 1320

Query: 1501 EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII 1560
            EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII
Sbjct: 1321 EIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKFFQCCPEQHFVHILEPFLKII 1380

Query: 1561 TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK 1612
            TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK
Sbjct: 1381 TKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKAVYEHHPRPKQLIVENDLPHK 1400

BLAST of Cp4.1LG02g15870 vs. ExPASy TrEMBL
Match: A0A6J1GB17 (MAP3K epsilon protein kinase 1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452538 PE=4 SV=1)

HSP 1 Score: 2523 bits (6538), Expect = 0.0
Identity = 1333/1497 (89.04%), Postives = 1339/1497 (89.45%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED
Sbjct: 1    MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LNVIM                       QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE
Sbjct: 61   LNVIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKLTEADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLTEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS
Sbjct: 301  PMPALFRIVQDKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGTLRNTQLDGSPG EISNGDDQNSCESPSAEKNEIAGSDFKADSRK+LASDVATD
Sbjct: 361  SLRHSGTLRNTQLDGSPGEEISNGDDQNSCESPSAEKNEIAGSDFKADSRKDLASDVATD 420

Query: 581  TSKSQKHFASG-HFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHESH 640
            TS SQKHFASG +FDEEGE IEEDT LDQVPTLSIRDNLSLLTGSGRLATSEPTE HESH
Sbjct: 421  TSNSQKHFASGPNFDEEGEAIEEDTLLDQVPTLSIRDNLSLLTGSGRLATSEPTEFHESH 480

Query: 641  GRAHDEVIMNGGVPSAELRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKMSIA 700
            GRAHDEVIMNGGVPSAELRKVASRK+GEKETSTTSGNSSFGFGPEGHDNCFQK SKMSIA
Sbjct: 481  GRAHDEVIMNGGVPSAELRKVASRKHGEKETSTTSGNSSFGFGPEGHDNCFQKASKMSIA 540

Query: 701  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGKNDL 760
            SGGNELSKFSDTPGDASLDDLFQPLDKLPADRS+EASTSLSTPQSNIGNVPVNNVGKNDL
Sbjct: 541  SGGNELSKFSDTPGDASLDDLFQPLDKLPADRSSEASTSLSTPQSNIGNVPVNNVGKNDL 600

Query: 761  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKLPGENLYPLPAV 820
            ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEK+PGENLYPLPAV
Sbjct: 601  ATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDVDIDGLVFDEKMPGENLYPLPAV 660

Query: 821  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRIT 880
            EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRI 
Sbjct: 661  EFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPKTRI- 720

Query: 881  CSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 940
                                       PLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT
Sbjct: 721  ---------------------------PLVMGFAVPDRPREVRMEAAYFFQQLCQSSSLT 780

Query: 941  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 1000
            LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL
Sbjct: 781  LQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRISAKSGIL 840

Query: 1001 LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 1060
            LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL
Sbjct: 841  LRLINTLYSLNEATRLASITVGAGNPFDGFQRSRSGQLDSSHCIFSQYEASFPVPDQPDL 900

Query: 1061 LKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNATNEALGTSK 1120
            LKVRHGIVD HLSAGTPEPSRASSSHSQRSDANQSDHRHFSTD GRLSSNATNEALGTSK
Sbjct: 901  LKVRHGIVDHHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDPGRLSSNATNEALGTSK 960

Query: 1121 PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1180
            PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP
Sbjct: 961  PPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFLEPASNGFP 1020

Query: 1181 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1240
            TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG
Sbjct: 1021 TASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHASNEKKMNG 1080

Query: 1241 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGIASQTASGV 1300
            ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVG+KVEPLVSNEGAPSTSGIASQTASGV
Sbjct: 1081 ELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGRKVEPLVSNEGAPSTSGIASQTASGV 1140

Query: 1301 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1360
            LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH
Sbjct: 1141 LSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTTVKSYMCSH 1200

Query: 1361 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1420
            SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS
Sbjct: 1201 SLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLELKEGSLVS 1260

Query: 1421 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1480
            QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN
Sbjct: 1261 QIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLCDMAHASRN 1320

Query: 1481 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1540
            SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF
Sbjct: 1321 SREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKEAIKKLVKF 1380

Query: 1541 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1600
            FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA
Sbjct: 1381 FQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARLNLLKLIKA 1392

Query: 1601 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1612
            VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL
Sbjct: 1441 VYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALHINTVL 1392

BLAST of Cp4.1LG02g15870 vs. TAIR 10
Match: AT3G13530.1 (mitogen-activated protein kinase kinase kinase 7 )

HSP 1 Score: 1713.0 bits (4435), Expect = 0.0e+00
Identity = 977/1505 (64.92%), Postives = 1098/1505 (72.96%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            M+RQ+  + FHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIVQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LN IM                       QEIDLLKNLNHKNIVKYLGS KTK+HLHIILE
Sbjct: 61   LNTIM-----------------------QEIDLLKNLNHKNIVKYLGSSKTKTHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS ANIIKPNKFGPFPESLVA+YI+QVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSLANIIKPNKFGPFPESLVAVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKL EADVNTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLNEADVNTHSVVGTPYWMAPEVIEMSGVCAASDIWSVGCTVIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D +PPIPDSLSPDITDFLRQCFKKD+RQRPDAKTLLSHPW++N RRAL S
Sbjct: 301  PMPALFRIVQDDNPPIPDSLSPDITDFLRQCFKKDSRQRPDAKTLLSHPWIRNSRRALQS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGT++  + + +  +E  +   Q++ ES S E   I+    K DS+ +L   V   
Sbjct: 361  SLRHSGTIKYMK-EATASSEKDDEGSQDAAESLSGENVGIS----KTDSKSKLPL-VGVS 420

Query: 581  TSKSQKHFAS-GHFDEEG-EGIEEDTPLDQVPTLSIRDNLSLLTGSGRLATSEPTESHES 640
            + +S+K  ++     EEG +  E+D   DQVPTLSI +  S   G+       P +  + 
Sbjct: 421  SFRSEKDQSTPSDLGEEGTDNSEDDIMSDQVPTLSIHEKSSDAKGT-------PQDVSDF 480

Query: 641  HGRA-HDEVIMNGGVPSAELRKVASR-KYGEKETSTTSGNSSFGFGPEGHDNCFQKVSKM 700
            HG++   E   N    ++E RK  S  K+  KE S     +S  FG +G +   +K  K 
Sbjct: 481  HGKSERGETPENLVTETSEARKNTSAIKHVGKELSIPVDQTSHSFGRKGEERGIRKAVKT 540

Query: 701  SIASGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNNVGK 760
              +  GNEL++FSD PGDASL DLF PLDK+   +  EASTS+ T   N G+ PV + GK
Sbjct: 541  PSSVSGNELARFSDPPGDASLHDLFHPLDKVSEGKPNEASTSMPTSNVNQGDSPVADGGK 600

Query: 761  NDLATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDV-DIDGLVFDEKLPGENLYP 820
            NDLATKLRATIAQKQME E G  N G DL RL+MGVL DDV DIDGLVFDEK+P ENL+P
Sbjct: 601  NDLATKLRATIAQKQMEGETGHSNDGGDLFRLMMGVLKDDVIDIDGLVFDEKVPAENLFP 660

Query: 821  LPAVEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLEVPK 880
            L AVEF RLV SLRPDE ED IVS+C KL+A+F  RPEQK+V+VTQHG LPL +LL++PK
Sbjct: 661  LQAVEFSRLVSSLRPDESEDAIVSSCQKLVAMFRQRPEQKVVFVTQHGFLPLMDLLDIPK 720

Query: 881  TRITCSVLQLINQIVKDNDDFQENACLVGLIPLVMGFAVP--DRPREVRMEAAYFFQQLC 940
            +R+ C+VLQLIN+I+KDN DFQENACLVGLIP+VM FA P  DR RE+R EAAYF QQLC
Sbjct: 721  SRVICAVLQLINEIIKDNTDFQENACLVGLIPVVMSFAGPERDRSREIRKEAAYFLQQLC 780

Query: 941  QSSSLTLQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFCRIS 1000
            QSS LTLQMF+ACRGIPVLV FLEADYAKYREMVHLAIDGMWQ+FKL+RST RN FCRI+
Sbjct: 781  QSSPLTLQMFIACRGIPVLVGFLEADYAKYREMVHLAIDGMWQVFKLKRSTPRNDFCRIA 840

Query: 1001 AKSGILLRLINTLYSLNEATRLASITVGAGNPFDG-FQRSRSGQLDSSHCIFSQYE-ASF 1060
            AK+GILLRLINTLYSLNEATRLASI+ G     DG   R RSGQLD ++ IF Q E +S 
Sbjct: 841  AKNGILLRLINTLYSLNEATRLASISGG----LDGQAPRVRSGQLDPNNPIFGQNETSSL 900

Query: 1061 PVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSNAT 1120
             + DQPD+LK RHG        G  EPS AS+S+SQRSD +Q D  H   D  R+SS A 
Sbjct: 901  SMIDQPDVLKTRHG--------GGEEPSHASTSNSQRSDVHQPDALHPDGDKPRVSSVA- 960

Query: 1121 NEALGTSKPPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPKFL 1180
                                     P    S   +   + RI   +++NRTSTD+  K  
Sbjct: 961  -------------------------PDASTSGTEDVRQQHRIS--LSANRTSTDKLQKLA 1020

Query: 1181 EPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLLHA 1240
            E ASNGFP    VT  EQVRPLLSLLDKEPPSRH SGQL+Y+ H++G+ERHE+ +PLLH 
Sbjct: 1021 EGASNGFP----VTQTEQVRPLLSLLDKEPPSRHYSGQLDYVKHITGIERHESRLPLLHG 1080

Query: 1241 SNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTSGI 1300
            SNEKK NG+LDFLMAEFAEVS RGKENG+LD T++   KT+ KKV   ++ EG  STSGI
Sbjct: 1081 SNEKKNNGDLDFLMAEFAEVSGRGKENGSLDTTTRYPSKTMTKKV---LAIEGVASTSGI 1140

Query: 1301 ASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQADTT 1360
            ASQTASGVLSGSGVLNA PGS+TSSGLL+HMVST++ADVAREYL KV+DLLLEFA+ADTT
Sbjct: 1141 ASQTASGVLSGSGVLNARPGSATSSGLLAHMVSTLSADVAREYLEKVADLLLEFARADTT 1200

Query: 1361 VKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPNLE 1420
            VKSYMCS SLL+RLFQMFNRVEP ILLKIL+C NHLSTDPNCLENLQRADAIK+LIPNLE
Sbjct: 1201 VKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHLSTDPNCLENLQRADAIKHLIPNLE 1260

Query: 1421 LKEGSLVSQIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPLLC 1480
            LK+G LV QIH EVLS LFNLCKINKRRQE A ENGIIPHLM FIMSDSPLK+ ALPLLC
Sbjct: 1261 LKDGHLVYQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLMLFIMSDSPLKQYALPLLC 1320

Query: 1481 DMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLKKE 1540
            DMAHASRNSREQLRAHGGLDVYLSLL+DE WSV ALDSIAVCLA DNDNRKVEQALLK++
Sbjct: 1321 DMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIALDSIAVCLAQDNDNRKVEQALLKQD 1368

Query: 1541 AIKKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIARL 1600
            AI+KLV FFQ CPE+HFVHILEPFLKIITKS RIN TLAVNGLTPLLI+RLDHQDAIARL
Sbjct: 1381 AIQKLVDFFQSCPERHFVHILEPFLKIITKSYRINKTLAVNGLTPLLISRLDHQDAIARL 1368

Query: 1601 NLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKALH 1613
            NLLKLIKAVYEHHPRPKQLIVENDLP KLQNLIEERRDGQRSGGQVLVKQMATSLLKALH
Sbjct: 1441 NLLKLIKAVYEHHPRPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKALH 1368

BLAST of Cp4.1LG02g15870 vs. TAIR 10
Match: AT3G07980.1 (mitogen-activated protein kinase kinase kinase 6 )

HSP 1 Score: 1622.1 bits (4199), Expect = 0.0e+00
Identity = 946/1507 (62.77%), Postives = 1073/1507 (71.20%), Query Frame = 0

Query: 161  MSRQVPITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQED 220
            M+RQ+  + FHKSKTLDNKYMLGDEIGKGAYGRVY GLDLENGDFVAIKQVSLENI QED
Sbjct: 1    MARQMTSSQFHKSKTLDNKYMLGDEIGKGAYGRVYIGLDLENGDFVAIKQVSLENIGQED 60

Query: 221  LNVIMCSHWIFFPSLLLKLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILE 280
            LN IM                       QEIDLLKNLNHKNIVKYLGSLKTK+HLHIILE
Sbjct: 61   LNTIM-----------------------QEIDLLKNLNHKNIVKYLGSLKTKTHLHIILE 120

Query: 281  YVENGSFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEAR 340
            YVENGS ANIIKPNKFGPFPESLV +YI+QVLEGLVYLHEQGVIHRDIKGANILTTKE  
Sbjct: 121  YVENGSLANIIKPNKFGPFPESLVTVYIAQVLEGLVYLHEQGVIHRDIKGANILTTKE-- 180

Query: 341  HTRTASLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADF 400
                                                                GLVKLADF
Sbjct: 181  ----------------------------------------------------GLVKLADF 240

Query: 401  GVATKLTEADVNTHSVVGTPYWMAPE---------------------------------- 460
            GVATKL EAD NTHSVVGTPYWMAPE                                  
Sbjct: 241  GVATKLNEADFNTHSVVGTPYWMAPEVIELSGVCAASDIWSVGCTIIELLTCVPPYYDLQ 300

Query: 461  ----------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHS 520
                      D  PPIPDSLSPDITDFLR CFKKD+RQRPDAKTLLSHPW++N RRAL S
Sbjct: 301  PMPALYRIVQDDTPPIPDSLSPDITDFLRLCFKKDSRQRPDAKTLLSHPWIRNSRRALRS 360

Query: 521  SLRHSGTLRNTQLDGSPGAEISNGDDQNSCESPSAEKNEIAGSDFKADSRKELASDVATD 580
            SLRHSGT+R  +   S   + + G  Q   ES SAEK E+     K +S+ +L       
Sbjct: 361  SLRHSGTIRYMKETDSSSEKDAEG-SQEVVESVSAEKVEVT----KTNSKSKLPVIGGAS 420

Query: 581  TSKSQKHFASGHFDEEGEGIEEDTPLDQVPTLSIRDNLSLLTGSGRLA------TSEPTE 640
                +   +     EEG   E+D   DQ PTLS+ D  S  +G+  ++      + +  E
Sbjct: 421  FRSEKDQSSPSDLGEEGTDSEDDINSDQGPTLSMHDKSSRQSGTCSISSDAKGTSQDVLE 480

Query: 641  SHESHGRAHDEVIMNGGVPSAE-LRKVASRKYGEKETSTTSGNSSFGFGPEGHDNCFQKV 700
            +HE + R  DE+  N    ++E  R   + K   KE S  S +S   F  +G D   +K 
Sbjct: 481  NHEKYDR--DEIPGNLETEASEGRRNTLATKLVGKEYSIQSSHS---FSQKGEDG-LRKA 540

Query: 701  SKMSIASGGNELSKFSDTPGDASLDDLFQPLDKLPADRSAEASTSLSTPQSNIGNVPVNN 760
             K   + GGNEL++FSD PGDASL DLF PLDK+P  ++ EASTS  T   N G+ PV +
Sbjct: 541  VKTPSSFGGNELTRFSDPPGDASLHDLFHPLDKVPEGKTNEASTSTPTANVNQGDSPVAD 600

Query: 761  VGKNDLATKLRATIAQKQMENEMGQVNGGDDLLRLVMGVLNDDV-DIDGLVFDEKLPGEN 820
             GKNDLATKLRA IAQKQME E G    G DL RL+MGVL DDV +ID LVFDEK+P EN
Sbjct: 601  GGKNDLATKLRARIAQKQMEGETGHSQDGGDLFRLMMGVLKDDVLNIDDLVFDEKVPPEN 660

Query: 821  LYPLPAVEFGRLVGSLRPDEPEDVIVSACHKLIAIFHHRPEQKIVYVTQHGLLPLTELLE 880
            L+PL AVEF RLV SLRPDE ED IV++  KL+A+F  RP QK V+VTQ+G LPL +LL+
Sbjct: 661  LFPLQAVEFSRLVSSLRPDESEDAIVTSSLKLVAMFRQRPGQKAVFVTQNGFLPLMDLLD 720

Query: 881  VPKTRITCSVLQLINQIVKDNDDFQENACLVGLIPLVMGFA--VPDRPREVRMEAAYFFQ 940
            +PK+R+ C+VLQLIN+IVKDN DF ENACLVGLIPLVM FA    DR RE+R EAAYF Q
Sbjct: 721  IPKSRVICAVLQLINEIVKDNTDFLENACLVGLIPLVMSFAGFERDRSREIRKEAAYFLQ 780

Query: 941  QLCQSSSLTLQMFVACRGIPVLVSFLEADYAKYREMVHLAIDGMWQIFKLQRSTLRNSFC 1000
            QLCQSS LTLQMF++CRGIPVLV FLEADYAK+REMVHLAIDGMWQ+FKL++ST RN FC
Sbjct: 781  QLCQSSPLTLQMFISCRGIPVLVGFLEADYAKHREMVHLAIDGMWQVFKLKKSTSRNDFC 840

Query: 1001 RISAKSGILLRLINTLYSLNEATRLASITVGAGNPFDG-FQRSRSGQLDSSHCIFSQYEA 1060
            RI+AK+GILLRL+NTLYSL+EATRLASI+ G     DG   R+RSGQLD ++ IFSQ E 
Sbjct: 841  RIAAKNGILLRLVNTLYSLSEATRLASIS-GDALILDGQTPRARSGQLDPNNPIFSQRET 900

Query: 1061 SFPVPDQPDLLKVRHGIVDPHLSAGTPEPSRASSSHSQRSDANQSDHRHFSTDAGRLSSN 1120
            S  V D PD LK R+G        G  EPS A +S+SQ SD +Q D  H   D  RLSS 
Sbjct: 901  SPSVIDHPDGLKTRNG--------GGEEPSHALTSNSQSSDVHQPDALHPDGDRPRLSS- 960

Query: 1121 ATNEALGTSKPPELASLEKVVNLATKEPSGFVSKEHENVDRWRIEQMVNSNRTSTDRPPK 1180
                               VV  AT++    V ++H      RI   +++NRTSTD+  K
Sbjct: 961  -------------------VVADATED----VIQQH------RIS--LSANRTSTDKLQK 1020

Query: 1181 FLEPASNGFPTASMVTPQEQVRPLLSLLDKEPPSRHLSGQLEYMHHLSGLERHETIMPLL 1240
              E ASNGFP    VT  +QVRPLLSLL+KEPPSR +SGQL+Y+ H++G+ERHE+ +PLL
Sbjct: 1021 LAEGASNGFP----VTQPDQVRPLLSLLEKEPPSRKISGQLDYVKHIAGIERHESRLPLL 1080

Query: 1241 HASNEKKMNGELDFLMAEFAEVSQRGKENGNLDPTSKVSLKTVGKKVEPLVSNEGAPSTS 1300
            +AS+EKK NG+L+F+MAEFAEVS RGKENGNLD   + S KT+ KKV   ++ E   ST 
Sbjct: 1081 YASDEKKTNGDLEFIMAEFAEVSGRGKENGNLDTAPRYSSKTMTKKV---MAIERVASTC 1140

Query: 1301 GIASQTASGVLSGSGVLNAIPGSSTSSGLLSHMVSTMNADVAREYLAKVSDLLLEFAQAD 1360
            GIASQTASGVLSGSGVLNA PGS+TSSGLL+H +S   ADV+ +YL KV+DLLLEFA+A+
Sbjct: 1141 GIASQTASGVLSGSGVLNARPGSTTSSGLLAHALS---ADVSMDYLEKVADLLLEFARAE 1200

Query: 1361 TTVKSYMCSHSLLNRLFQMFNRVEPSILLKILKCINHLSTDPNCLENLQRADAIKYLIPN 1420
            TTVKSYMCS SLL+RLFQMFNRVEP ILLKIL+C NHLSTDPNCLENLQRADAIK LIPN
Sbjct: 1201 TTVKSYMCSQSLLSRLFQMFNRVEPPILLKILECTNHLSTDPNCLENLQRADAIKQLIPN 1260

Query: 1421 LELKEGSLVSQIHTEVLSTLFNLCKINKRRQECAIENGIIPHLMHFIMSDSPLKKDALPL 1480
            LELKEG LV QIH EVLS LFNLCKINKRRQE A ENGIIPHLM F+MSDSPLK+ ALPL
Sbjct: 1261 LELKEGPLVYQIHHEVLSALFNLCKINKRRQEQAAENGIIPHLMLFVMSDSPLKQYALPL 1320

Query: 1481 LCDMAHASRNSREQLRAHGGLDVYLSLLEDEIWSVTALDSIAVCLAHDNDNRKVEQALLK 1540
            LCDMAHASRNSREQLRAHGGLDVYLSLL+DE WSV ALDSIAVCLA D D +KVEQA LK
Sbjct: 1321 LCDMAHASRNSREQLRAHGGLDVYLSLLDDEYWSVIALDSIAVCLAQDVD-QKVEQAFLK 1367

Query: 1541 KEAIKKLVKFFQCCPEQHFVHILEPFLKIITKSSRINTTLAVNGLTPLLIARLDHQDAIA 1600
            K+AI+KLV FFQ CPE+HFVHILEPFLKIITKSS IN TLA+NGLTPLLIARLDHQDAIA
Sbjct: 1381 KDAIQKLVNFFQNCPERHFVHILEPFLKIITKSSSINKTLALNGLTPLLIARLDHQDAIA 1367

Query: 1601 RLNLLKLIKAVYEHHPRPKQLIVENDLPHKLQNLIEERRDGQRSGGQVLVKQMATSLLKA 1613
            RLNLLKLIKAVYE HP+PKQLIVENDLP KLQNLIEERRDGQRSGGQVLVKQMATSLLKA
Sbjct: 1441 RLNLLKLIKAVYEKHPKPKQLIVENDLPQKLQNLIEERRDGQRSGGQVLVKQMATSLLKA 1367

BLAST of Cp4.1LG02g15870 vs. TAIR 10
Match: AT3G06030.1 (NPK1-related protein kinase 3 )

HSP 1 Score: 159.1 bits (401), Expect = 3.0e-38
Identity = 112/366 (30.60%), Postives = 164/366 (44.81%), Query Frame = 0

Query: 167 ITAFHKSKTLDNKYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSLENIAQEDLNVIMC 226
           + A  K +    ++  G+ IG GA+GRVY G++L++G+ +AIKQV               
Sbjct: 55  LPAPRKEEAPSIRWRKGELIGCGAFGRVYMGMNLDSGELLAIKQV--------------- 114

Query: 227 SHWIFFPSLLLK-LLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENG 286
              +  PS   K    G   + ++E+ LLKNL+H NIV+YLG+++    L+I++E+V  G
Sbjct: 115 ---LIAPSSASKEKTQGHIRELEEEVQLLKNLSHPNIVRYLGTVRESDSLNILMEFVPGG 174

Query: 287 SFANIIKPNKFGPFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEARHTRTA 346
           S +++++  KFG FPE ++ +Y  Q+L GL YLH  G++HRDIKGANIL   +       
Sbjct: 175 SISSLLE--KFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNK------- 234

Query: 347 SLRLKSKEKIYFFEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADFGVATK 406
                                                          G ++LADFG + K
Sbjct: 235 -----------------------------------------------GCIRLADFGASKK 294

Query: 407 LTE-ADVN-THSVVGTPYWMAPE------------------------------------- 466
           + E A VN   S+ GTPYWMAPE                                     
Sbjct: 295 VVELATVNGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQF 346

Query: 467 ---------DKHPPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWVQNCRRALHSS 484
                      HPPIP+ LSP+  DFL +C  K+   R  A  LL HP+V   R+  + +
Sbjct: 355 AAVLHIGRTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHPFVTGKRQEPYPA 346

BLAST of Cp4.1LG02g15870 vs. TAIR 10
Match: AT1G09000.1 (NPK1-related protein kinase 1 )

HSP 1 Score: 154.5 bits (389), Expect = 7.5e-37
Identity = 112/374 (29.95%), Postives = 162/374 (43.32%), Query Frame = 0

Query: 183 GDEIGKGAYGRVYKGLDLENGDFVAIKQVSL-ENIAQEDLNVIMCSHWIFFPSLLLKLLV 242
           G  IG+GA+G VY G++L++G+ +A+KQV +  N A ++                     
Sbjct: 72  GQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHI--------------- 131

Query: 243 GCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFGPFPE 302
               + ++E+ LLKNL+H NIV+YLG+++    L+I+LE+V  GS +++++  KFGPFPE
Sbjct: 132 ---QELEEEVKLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLE--KFGPFPE 191

Query: 303 SLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEARHTRTASLRLKSKEKIYFFEGC 362
           S+V  Y  Q+L GL YLH   ++HRDIKGANIL   +                       
Sbjct: 192 SVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNK----------------------- 251

Query: 363 FTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADFGVATKLTEADVNT--HSVVGT 422
                                          G +KLADFG + ++ E    T   S+ GT
Sbjct: 252 -------------------------------GCIKLADFGASKQVAELATMTGAKSMKGT 311

Query: 423 PYWMAPE----------------------------------------------DKHPPIP 482
           PYWMAPE                                                HPPIP
Sbjct: 312 PYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIP 371

Query: 483 DSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWV-----QNCRRALHSSLRHSGTLRNTQ 503
           D+LS D  DFL +C ++    RP A  LL HP+V     ++    L S L +  T    Q
Sbjct: 372 DTLSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQ 371

BLAST of Cp4.1LG02g15870 vs. TAIR 10
Match: AT1G54960.1 (NPK1-related protein kinase 2 )

HSP 1 Score: 153.3 bits (386), Expect = 1.7e-36
Identity = 108/378 (28.57%), Postives = 166/378 (43.92%), Query Frame = 0

Query: 179 KYMLGDEIGKGAYGRVYKGLDLENGDFVAIKQVSL-ENIAQEDLNVIMCSHWIFFPSLLL 238
           ++  G  IG+GA+G VY G++L++G+ +A+KQV +  N A ++                 
Sbjct: 22  RWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHI----------- 81

Query: 239 KLLVGCNDDFQQEIDLLKNLNHKNIVKYLGSLKTKSHLHIILEYVENGSFANIIKPNKFG 298
                   + ++E+ LLKNL+H NIV+YLG+++    L+I+LE+V  GS +++++  KFG
Sbjct: 82  -------QELEEEVKLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLE--KFG 141

Query: 299 PFPESLVALYISQVLEGLVYLHEQGVIHRDIKGANILTTKEARHTRTASLRLKSKEKIYF 358
            FPES+V  Y +Q+L GL YLH   ++HRDIKGANIL   +                   
Sbjct: 142 AFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQ------------------- 201

Query: 359 FEGCFTDLFLISQKEEATVADCWNKDQNDWDLGLGGLVKLADFGVATKLTEADV--NTHS 418
                                              G +KLADFG + ++ E        S
Sbjct: 202 -----------------------------------GCIKLADFGASKQVAELATISGAKS 261

Query: 419 VVGTPYWMAPE----------------------------------------------DKH 478
           + GTPYWMAPE                                                H
Sbjct: 262 MKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSH 321

Query: 479 PPIPDSLSPDITDFLRQCFKKDARQRPDAKTLLSHPWV-----QNCRRALHSSLRHSGTL 503
           PPIPD++S D  DFL +C +++   RP A  LL HP+V     ++  + L S + +S + 
Sbjct: 322 PPIPDNISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASKDLTSFMDNSCSP 325