Cp4.1LG02g15830 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g15830 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 2.5e-89
Identity = 173/338 (51.18%), Postives = 232/338 (68.64%), Query Frame = 0

Query: 7   NFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGGSTSFFI 66
           +FIK W  V ISLCY     KL+P G  R +   P+ ++FF++P  + S+HL G T+FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSA-EQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDTTLKPK 126
           AWLANFKLLLFA G GPLS+  +PL L  FLA+SCLPIKI  + K    ++   +T  P 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGP- 122

Query: 127 SFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRNVLGIE 186
             L Y+IKAV V L++K Y+YS  +  KVVL +Y+ H+YF LEIILA T    R +  +E
Sbjct: 123 --LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 182

Query: 187 LEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPAVMATF 246
           LEPQF  PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   TF
Sbjct: 183 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTF 242

Query: 247 IVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLPPAVSVPVTL 306
           +VS +MHELIF+YMGR+RP+W+  WFF+I+G   TVEI +KK +  +WR P A+S  +TL
Sbjct: 243 VVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTL 302

Query: 307 AFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNV 344
            F+  TA+WLF P+F R  +  +A +EYAA+  F   V
Sbjct: 303 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

BLAST of Cp4.1LG02g15830 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 3.0e-71
Identity = 151/345 (43.77%), Postives = 209/345 (60.58%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           M+ E+ N IKVW++  IS+ YC+     +  GA RFL+V+P++ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLR--RFLALSCLPIKILQNEKSCPNNNRS 120
            T+FF+ WLANFKL+LF+   GPL    P+P    RF   +C PIK+ QN KS       
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKS------- 120

Query: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180
                PK  L ++IK  I A+++ LY Y + + P ++L +Y  HLY  +EIIL   +   
Sbjct: 121 -QNHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKK--ALPEKWRLP 300
           P ++A FIVS ++HEL+F+Y+ R  P  E T FFV+HGV   VE+ VKK   + ++WRL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVK 342
           P VS  +T+ F+F T  WLF PQ  R  +  R   E   LV F+K
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Cp4.1LG02g15830 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 3.7e-69
Identity = 139/350 (39.71%), Postives = 210/350 (60.00%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLP-PGAGRFLAVAPIVVLFFLLPLQLTSIHLG 60
           +E E+  F +VW++   + CYC     + P  GA R L + P+V+LF  LPL+L+S HLG
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GSTSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNEKSCPNNNRSD 120
           G T+ ++ WLANFKLLLFA   GPLS    L L  F++ + LPIK   +  +    N+  
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKRT 122

Query: 121 TTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGR 180
            + + +  + +  K V  A ++K+Y++ + +   V+  +Y +H Y   EI LA +    R
Sbjct: 123 LSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIAR 182

Query: 181 NVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLP 240
             LG++L PQF +PY +TSLQDFWGRRWNLM ++IL  T Y+P  ++ ++ +  +W    
Sbjct: 183 ATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAG 242

Query: 241 AVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLPPAV 300
           A++  F VS +MHE+ F+Y+ R RP+WE T FFV+HGV   VE++VKKA+  K RL   V
Sbjct: 243 AMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREV 302

Query: 301 SVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKG 350
           S  +T+ F+  T  WLF PQ VR  +D++  +EY  +  + +     L G
Sbjct: 303 SGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

BLAST of Cp4.1LG02g15830 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 1.0e-66
Identity = 144/348 (41.38%), Postives = 204/348 (58.62%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           ME E+++ IKVW+   IS+ YC+ T   +  G  R L+V P+ VLF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDT 120
           ST+FF++WLANFKL+LF+   GPL    P  L RF+  +C PIK+ QN K  P N     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQNPK--PQNQ---- 120

Query: 121 TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRN 180
              PK    +++K     +++ +Y+Y + + P V+L +YS H+Y   EI+LA  +     
Sbjct: 121 --MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSI 180

Query: 181 VLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPA 240
            L  +LEP F +PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A    
Sbjct: 181 SLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLG 240

Query: 241 VMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA-LPEKWRLPPAV 300
           V A+F+VS ++HELIF+Y  R  P  E T FFV+HGV    E   K+  L  +W++   V
Sbjct: 241 VFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMV 300

Query: 301 SVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSL 348
           S  +T+ F+  T  WLFFP   R  +  R  +E    + FVK+  F L
Sbjct: 301 SRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

BLAST of Cp4.1LG02g15830 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 9.5e-65
Identity = 136/346 (39.31%), Postives = 205/346 (59.25%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           ME E+ NFIK+W++  IS+ YC+     +  G  R L+V P+  LF + PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLP--LRRFLALSCLPIKILQNEKSCPNNNRS 120
             +FF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK        P  N +
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIK--------PQQNPN 120

Query: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180
               K   +L ++IK VI  +++++Y+Y +++ P ++L   S H++  LEI+  + +   
Sbjct: 121 IQNYKIPIWL-FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALV 180

Query: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240
              LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + +    A  
Sbjct: 181 FITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMF 240

Query: 241 PAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA--LPEKWRLP 300
             V A F+VS  +HE++F+Y+ R  P  E TWFF++HGV    E+ VKK+  +   WR+ 
Sbjct: 241 LGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVS 300

Query: 301 PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKN 343
           P VS  +T+ F+  T+ W FFP  +R  +  R   E    + FVK+
Sbjct: 301 PTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVKH 333

BLAST of Cp4.1LG02g15830 vs. NCBI nr
Match: KAG7036600.1 (Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1622 bits (4199), Expect = 0.0
Identity = 861/1106 (77.85%), Postives = 866/1106 (78.30%), Query Frame = 0

Query: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
            MEGEILNFIKVWLTVCISLCYCHATGKLL PG GRFLAVAPIVVLFFLLPLQLTSIHLGG
Sbjct: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61   STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDT 120
            STSFFIAWLANFKLLLFAAGDGPLSAE PLPLRRFLALSCLPIKILQNEKSCPNNNRSDT
Sbjct: 61   STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQNEKSCPNNNRSDT 120

Query: 121  TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRN 180
            TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIY+                    
Sbjct: 121  TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYT-------------------- 180

Query: 181  VLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPA 240
                                                                        
Sbjct: 181  ------------------------------------------------------------ 240

Query: 241  VMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLPPAVS 300
                                                             P K        
Sbjct: 241  -------------------------------------------------PAKCG------ 300

Query: 301  VPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKGIEKKKQSVIGR 360
                                                                        
Sbjct: 301  ------------------------------------------------------------ 360

Query: 361  RSSGERMEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLH 420
                 RMEDEIGKLLKVWISIW+CLGYCYGVG KIGEGFTRFL IFPVICIFLYLPLNLH
Sbjct: 361  -----RMEDEIGKLLKVWISIWMCLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLNLH 420

Query: 421  SLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSE 480
            SLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIP QKSSE
Sbjct: 421  SLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPSQKSSE 480

Query: 481  PAGNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIY 540
            PA NGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIY
Sbjct: 481  PANNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIY 540

Query: 541  FILDLSFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAV 600
            FILDLSFAAASAAVSH GT+ELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAV
Sbjct: 541  FILDLSFAAASAAVSHAGTMELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAV 600

Query: 601  STATKLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVE 660
            STATKLIGPRWAPLAGVA TFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVE
Sbjct: 601  STATKLIGPRWAPLAGVAVTFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVE 660

Query: 661  MVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA 720
            MVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA
Sbjct: 661  MVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGA 720

Query: 721  -------------------------FSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKNSI 780
                                     FSDSPLMAAEILIFLQTLLQVSSSLCFC+SI+NSI
Sbjct: 721  GVRRRKGKRKKKNRAPFLKSFSISSFSDSPLMAAEILIFLQTLLQVSSSLCFCFSIRNSI 780

Query: 781  PNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAAA 840
            PNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSA A
Sbjct: 781  PNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAVA 840

Query: 841  SSSLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPN 900
            SSSLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPN
Sbjct: 841  SSSLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPN 900

Query: 901  GTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSR 960
            GTLIVFCFVIYLLLEIVLGG AAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSR
Sbjct: 901  GTLIVFCFVIYLLLEIVLGGVAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSR 906

Query: 961  ILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFL 1020
            ILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFL
Sbjct: 961  ILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFL 906

Query: 1021 VHGFFLAVEMAVKNAVGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEH 1080
            VHGFFLAVEMAVKNAVGERFRPPR VTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEH
Sbjct: 1021 VHGFFLAVEMAVKNAVGERFRPPRIVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEH 906

BLAST of Cp4.1LG02g15830 vs. NCBI nr
Match: KAA0048417.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 461/718 (64.21%), Postives = 526/718 (73.26%), Query Frame = 0

Query: 367  MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 426
            ME+EIG  LKVWIS WICL YCYGVG +I  G TRFLFI PV+ +FLYLPL LHSLHLGG
Sbjct: 1    MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 427  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 486
            LTAFF+AWLANFKLLLF+AG GPL+  G+S+  FCA+ASFP+K+KQ P QK+SE A NG 
Sbjct: 61   LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 120

Query: 487  AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 546
                +  KS+ N H +SP +YAVKI LVAA+VKAY+YTHILPQKL W  LCFHIYF+L+L
Sbjct: 121  ----LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 547  SFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 606
            S AAA++ V+ GGT+ELLPLFH PY STSLQDFWGRRWNL VTGILRP  + PA S A+K
Sbjct: 181  SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 607  LIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 666
            L G RW P+ GV ATF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMV KR
Sbjct: 241  LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 667  LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFSDSP 726
            +WI  W M PLP+ V+VPVTVGFFFW+CFWLFFPPFIN+CKADVRVV+EY +FG F    
Sbjct: 301  VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF---- 360

Query: 727  LMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQ 786
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 787  GAIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLLVGSLPIDIYDHDPASTRP-DT 846
               GFFIGWLASFKLLLFAFGKGPL S AASSSLRRFL +GSLPI+I    P  T P D 
Sbjct: 421  ---GFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEI----PQDTNPSDP 480

Query: 847  NSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATL 906
            N +LP +   KL  L L+  A+Y + Y HPN  LI FCF+IY LLEI++GGAAA  KATL
Sbjct: 481  NPILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATL 540

Query: 907  GVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATT 966
            GVELLP FDEPYLS+SLQDFWG+RWNLM SRILRL+VYDPCR     IVG+KP+AI+A T
Sbjct: 541  GVELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMT 600

Query: 967  ATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRFVTVP 1026
            ATFAVSGLMHELIYFY+GR+APTWEVT FFL+HG  L+ EMA+K+AVG RFR PR V+  
Sbjct: 601  ATFAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTG 643

Query: 1027 LTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVK-LISFQVK 1081
             T  FVM TS WLFFPQFFR KP+VRMFEEHAA+ AFVKNV  +LIT  K L+ F +K
Sbjct: 661  FTWWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 643

BLAST of Cp4.1LG02g15830 vs. NCBI nr
Match: KAF4379219.1 (hypothetical protein G4B88_010613 [Cannabis sativa])

HSP 1 Score: 823 bits (2127), Expect = 7.13e-275
Identity = 469/1067 (43.96%), Postives = 639/1067 (59.89%), Query Frame = 0

Query: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
            MEGEI N + VW+TV  SLCYCH  G ++  G  R + + P++++F +LPL L +I LGG
Sbjct: 1    MEGEINNLVMVWITVVASLCYCHTIGNIITSGTTRLIPILPVILIFLVLPLSLRTIFLGG 60

Query: 61   STSFFIAWLANFKLLLFAAGDGPLSAEQPL---PLRRFLALSCLPIKILQNEKSCPNNNR 120
              SFF+AWLANFKLLLFA G GPLS+  PL   PL RF+AL+  PIKI  +    P N +
Sbjct: 61   PISFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSRFIALASFPIKIQDD----PINKQ 120

Query: 121  SDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFI-HPKVVLFIYSWHLYFLLEIILAITQF 180
                   KS  NY+IKA+I + ++ +     +I +PKV L ++  ++Y  LEI+LA    
Sbjct: 121  K------KSLTNYAIKALIFSTIIPVQQNKSYIIYPKVFLSLHFIYIYTFLEIMLATVAA 180

Query: 181  FGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWA 240
              R  LG+ELEP F++PY STSLQDFWGRRWNLMA++ L+ TVY P   I ++ IGRKWA
Sbjct: 181  LVRATLGVELEPHFEEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRTIMSRWIGRKWA 240

Query: 241  PLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLP 300
            P+PAV+ TF+VS  +HE IFY +GR +P WE T FFV+HG+ L  EI VKKA+  KWRLP
Sbjct: 241  PVPAVIPTFMVSGFIHEFIFYNLGRSKPRWEVTLFFVVHGICLAAEIAVKKAINRKWRLP 300

Query: 301  PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKGIEKKKQS 360
            P V+  + LAF+  T +WLF P  +RL++DV+A  E    +  +K   + +        S
Sbjct: 301  PLVTGSMALAFVMSTVLWLFMPDLMRLEIDVKAHREMIEFLESIKMTRYQI-------GS 360

Query: 361  VIGRRSSGER--MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLY 420
             I    + E+  ME EI  L+ VWI+  + L YC+ +GN I  G TR + I PVI IFL 
Sbjct: 361  TIKAEVTREKILMESEINNLVMVWITAVVSLCYCHTIGNIITPGTTRLIPILPVILIFLV 420

Query: 421  LPLNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTG----MSIAEFCAVASFPIKI 480
            LPLNL ++ L     FFVAWLANFKLLLFA G+GPL++      + ++ F  +ASFPIKI
Sbjct: 421  LPLNLRTISLRVPILFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSRFITLASFPIKI 480

Query: 481  KQIPPQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKL 540
            +                    DP  N+  ++S  NYA+K L+++ ++        +   +
Sbjct: 481  QD-------------------DPIINKQ-KKSLTNYAIKALILSTIIPIQQNKSSIHPNV 540

Query: 541  VWFLLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGI 600
              F  C + Y IL++  A  +A V     +EL P F  PYLSTSLQDFWGRRWNL  +  
Sbjct: 541  FLFFYCIYNYIILEIILATVAALVRAALGVELEPNFEEPYLSTSLQDFWGRRWNLIASDS 600

Query: 601  LRPVAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFF 660
            LR   Y P  +  ++ IG +WAP+  V  TF+VS  +HE +F+ M R    PTWEV  FF
Sbjct: 601  LRSTVYIPVRTMMSRWIGRKWAPVPAVIPTFMVSGIIHEFIFYNMRRS-KQPTWEVTLFF 660

Query: 661  VLHGGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVR 720
            V+HG CLA E+  K+     W++PP+   VS  + V F   T  WLF P  +   +ADV 
Sbjct: 661  VVHGMCLAAEIAVKKAVNRKWRLPPV---VSGSLAVAFVMTTKLWLFMPA-LMALEADVN 720

Query: 721  VVKEYEIF--GAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPF 780
              +E   F      +  LM +EI   +   + V +SLC+C++I N I  G  + + ILP 
Sbjct: 721  AHREMIEFVESIKKEKILMESEINNLVMVWITVVASLCYCHTIGNIITPGTARLIAILPV 780

Query: 781  FLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLLVGSL 840
             L+FL +PL  +T+ L G I FF+ WLA+FKLLLFA+GKGPL S      L RF+ + S 
Sbjct: 781  ILIFLVLPLNLRTIILGGPISFFVAWLANFKLLLFAYGKGPLSSNPLPLPLSRFIPLASF 840

Query: 841  PIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNY-FHPNGTLIVFCFVIYL 900
            PI I + DP + +  + +    + + K    S  I     K+Y  HP   + ++   IY+
Sbjct: 841  PIKIQE-DPLNKQKKSLT----NYAIKALIFSTIIPVQQNKSYTIHPKVFMSLYFIYIYI 900

Query: 901  LLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRN 960
             LEI+    AA+ +A LG EL P FDEPYLS+SLQDFWGRRWNLM S  LR +VY P RN
Sbjct: 901  FLEIIQAAVAALVRAALGAELEPHFDEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRN 960

Query: 961  FAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAV 1020
              +  +GRK A + A   TF +SG++HE I++ + R  PTWEVT  F+VHG  LA E+AV
Sbjct: 961  IMSRWIGRKWAPVPAVIPTFMMSGIIHEFIFYSMRRSKPTWEVTLLFVVHGMCLAAEIAV 1020

Query: 1021 KNAVGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEE 1054
            K AV  ++R P  V+V L + F+M T+ WLF P   R + DV+ + E
Sbjct: 1021 KKAVNRKWRLPPLVSVSLAVAFLMTTALWLFMPALMRLEVDVKAYSE 1020

BLAST of Cp4.1LG02g15830 vs. NCBI nr
Match: KAF4352693.1 (hypothetical protein G4B88_009767 [Cannabis sativa])

HSP 1 Score: 818 bits (2114), Expect = 5.23e-268
Identity = 440/1037 (42.43%), Postives = 629/1037 (60.66%), Query Frame = 0

Query: 35   RFLAVAPIVVLFFLLPLQLTSIHLGGSTSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRR 94
            R     PIV LF  LPL L SI+L G T+FFI+WLANFKLLL A   GPL+ +       
Sbjct: 239  RLFLFLPIVSLFLFLPLNLYSINLCGGTAFFISWLANFKLLLLAFQKGPLALKNQ-SFGL 298

Query: 95   FLALSCLPIKILQNEKSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYD-YSEFIHPK 154
            F+A++CLPI+I +  K  P           +  +N+ IKA+++A++++  D Y + IHP 
Sbjct: 299  FIAIACLPIEI-KTTKETPK----------RQLINFPIKALLLAILIQFLDHYKQQIHPN 358

Query: 155  VVLFIYSWHLYFLLEIILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATN 214
            +VLF+Y + +Y LLEIILAIT    R V+G+E+EPQF +PY STSLQDFWGRRWNLM T 
Sbjct: 359  LVLFLYCFLIYLLLEIILAITAALARAVVGLEVEPQFNEPYLSTSLQDFWGRRWNLMVTR 418

Query: 215  ILQPTVYKPSVKIAAKVIGRKWAPLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFV 274
            IL+ TVY+P++ + + ++GRKWA +PA++ATF+VS ++HEL+++Y+ R+ P WE T FFV
Sbjct: 419  ILRHTVYQPAIGLISTLMGRKWASIPAIIATFLVSGLVHELMYFYLSRVPPTWEVTCFFV 478

Query: 275  IHGVALTVEIMVKKALPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEY 334
            +HG+ LTVEI++KKAL + WR P  +S  +T+ F+  T  WLFFPQF+R + DV+  +EY
Sbjct: 479  LHGICLTVEIILKKALGDTWRFPKVMSRMLTIGFVMLTGFWLFFPQFLRCKADVKLVQEY 538

Query: 335  AALVTFVKNVAFSLKGIEKKKQSVIGRRSSGERMEDEIGKLLKVWISIWICLGYCYGVGN 394
            A                                                          N
Sbjct: 539  A----------------------------------------------------------N 598

Query: 395  KIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATT 454
             + +G  R +   P++ +FL LPL L+S++LGG+TAFF++WLANFKLLLFA  +GPLA+ 
Sbjct: 599  FVSKGMKRLICFLPIVLLFLLLPLKLNSINLGGVTAFFISWLANFKLLLFAFEKGPLASN 658

Query: 455  GMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLV 514
              S+  F  +A  PI IK+ P                      Q  +    N+  KI+ +
Sbjct: 659  SNSLGLFVVIACLPIAIKENPSNNKM---------------IKQASKRHLINFPTKIIFL 718

Query: 515  AALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLST 574
              L++  DY   +   LV  + C  IY +L+   A A+        +EL P FH PYLST
Sbjct: 719  VVLLRVLDYKKQMHHNLVVLIYCPLIYLLLETILALAATLARALVRLELEPQFHEPYLST 778

Query: 575  SLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFF 634
            SL DFWG RWNL +  ILRP  Y P ++  + L+G +WAP+  +  TF+VS  +HEL+++
Sbjct: 779  SLHDFWGCRWNLMIRRILRPTIYEPTLNLVSPLVGRKWAPIPAILGTFVVSGLVHELIYY 838

Query: 635  YMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTC 694
            Y++R    PTWEV +FF+LHG C  +E+  K+  ID W++P +   +S  +TVGF   T 
Sbjct: 839  YIARV--TPTWEVTSFFILHGLCYTIEICLKKTLIDTWQIPKV---ISRMLTVGFVTLTG 898

Query: 695  FWLFFPPFINTCKADVRVVKEYEIFGAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKN 754
             WLFFP F   CKADV++V+EY  F        M  E+  F++       SL +CY +  
Sbjct: 899  IWLFFPQFFR-CKADVKLVQEYAYFERKKGKEKMEGEMNNFIEVWFLALVSLSYCYVMSK 958

Query: 755  SIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSA 814
             I  G  + +  LP   LFLY+PL+  +++L G   FF+ WLA+FKLLL AF KGPL ++
Sbjct: 959  LISKGIKRLIFFLPIICLFLYLPLKLNSVNLVGTTSFFLSWLANFKLLLLAFNKGPLATS 1018

Query: 815  AASS-SLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPL-SLSSKLFFLSLSIAAIYCKNY 874
              S  S  RF+++ S PI+I +++   ++  +N+ +P+ S + K   L++ I     K+ 
Sbjct: 1019 NPSPISFGRFIVIASFPIEIQENNTNPSKEKSNNKIPIISFTIKTLLLAILIRVCDYKDQ 1078

Query: 875  FHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNL 934
             +PN  L+++CF++YLLLE +L   A + +   GV L P F+EPYL++SLQDFWG+RWNL
Sbjct: 1079 INPNVVLVLYCFLVYLLLETILVFVAIMVRVVAGVGLQPQFNEPYLTTSLQDFWGKRWNL 1138

Query: 935  MVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVT 994
            MV+RILR +V++P   F++  +GRK A + A  ATF VSGL+HELIYFY+ R  PTWEVT
Sbjct: 1139 MVTRILRPTVFEPTLKFSSTFIGRKWAQLPAIVATFVVSGLVHELIYFYMIRTPPTWEVT 1184

Query: 995  SFFLVHGFFLAVEMAVKNAVGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRM 1054
             FF++HG  L VE+ +K  +G+ +R P  V+  LTIGFVM+TSFWLFFPQ  R K DVR 
Sbjct: 1199 LFFVLHGLCLTVEIVLKKCLGDTWRLPSLVSGALTIGFVMLTSFWLFFPQLLRCKADVRT 1184

Query: 1055 FEEHAAMGAFVKNVAAE 1068
             EE+A +G F++ +  +
Sbjct: 1259 LEEYANLGKFLRKMVTQ 1184

BLAST of Cp4.1LG02g15830 vs. NCBI nr
Match: KAF4399539.1 (hypothetical protein G4B88_022622, partial [Cannabis sativa])

HSP 1 Score: 801 bits (2069), Expect = 3.84e-264
Identity = 462/1076 (42.94%), Postives = 628/1076 (58.36%), Query Frame = 0

Query: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
            ++ EI N + VW+TV  SL YCH  G ++ PG  R + + P++++F +LPL L +I LGG
Sbjct: 663  IKSEINNLVMVWITVVASLYYCHTIGNIITPGTTRLIPILPVILVFLVLPLNLRTIFLGG 722

Query: 61   STSFFIAWLANFKLLLFAAGDGPLSAEQPL---PLRRFLALSCLPIKILQNEKSCPNNNR 120
              SFF+AWLANFKLLLF  G GPLS+  PL   PL  F+ L+  PIKIL N    P N +
Sbjct: 723  PISFFVAWLANFKLLLFVYGKGPLSSNNPLLPLPLSHFITLASFPIKILNN----PINKQ 782

Query: 121  SDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFI-HPKVVLFIYSWHLYFLLEIILAITQF 180
                   KS  NY+IKA+I + ++ +     +I +PKV L IY  ++Y  LEI+LA    
Sbjct: 783  K------KSLTNYAIKALIFSTIIPVQQNKSYIIYPKVFLSIYFIYIYTFLEIMLATVAA 842

Query: 181  FGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWA 240
              R  LG+ELEP F++PY STSLQDFWGRRWNLMA++ L+ TVY P   + ++ IGRKWA
Sbjct: 843  LVRAALGVELEPHFEEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRTVMSRWIGRKWA 902

Query: 241  PLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLP 300
            P+PAV+ TF+VS ++HE IFY +GR +P WE T FFV+HG+ L  EI VKKA+  KWRLP
Sbjct: 903  PVPAVIPTFMVSGIIHEFIFYNLGRSKPRWEVTLFFVVHGICLAAEIAVKKAINRKWRLP 962

Query: 301  PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKGIEKKKQS 360
            P V+  + LAF+  T +WLF P  +RL++D++A                     E  ++ 
Sbjct: 963  PLVTGSMALAFVMSTVLWLFMPDLIRLEVDIKA---------------------EVTREK 1022

Query: 361  VIGRRSSGERMEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLP 420
            ++        ME E+  L+ VWI++   L YC+ +G  I  G  R + I PVI IFL LP
Sbjct: 1023 IL--------MESELNNLVMVWITVVASLCYCHTIGKIITPGTARLIAILPVILIFLVLP 1082

Query: 421  LNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSI--AEFCAVASFPIKIKQIP 480
            LNL ++ LGG  +FFVAWLANFKLLLFA G+GPL++  + +  + F  +ASFPIKI+  P
Sbjct: 1083 LNLRTIILGGPISFFVAWLANFKLLLFAYGKGPLSSNPLPLPLSRFIPLASFPIKIQDDP 1142

Query: 481  PQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAY-DYTHILPQKLVWF 540
             +K                      ++S  NYA+K L+ + ++    + ++ +  K+   
Sbjct: 1143 LKKQ---------------------KKSLTNYAIKALIFSTIIPVQQNKSYSIHPKVFMS 1202

Query: 541  LLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRP 600
            L   +IY  L++  AA +A V      EL P F  PYLSTSLQDFWGRRWNL  +  LR 
Sbjct: 1203 LYFIYIYIFLEIIQAAVAALVRAALGAELEPHFDEPYLSTSLQDFWGRRWNLMASDSLRS 1262

Query: 601  VAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLH 660
              Y P  +  ++ IG +WAP+  V  TF++S  +HE +F+ M R    PTWEV   FV+H
Sbjct: 1263 TVYIPVRNIMSRWIGRKWAPVPAVIPTFMMSGIIHEFIFYSMRRS--KPTWEVTLLFVVH 1322

Query: 661  GGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVK 720
            G CLA E+  K+     W++PPL   VS  + V F   T  WLF P  +   + DV+   
Sbjct: 1323 GMCLAAEIAVKKAVNRKWRLPPL---VSGSLAVAFLMTTALWLFMPALMRL-EVDVKAYS 1382

Query: 721  EYEIFGAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPFFLLFL 780
            E                    +   + V +SLC+C++I   I  G  + + ILP  L+FL
Sbjct: 1383 EINNL----------------VMVWITVVASLCYCHTIGKIITPGTTRLIPILPVILVFL 1442

Query: 781  YIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCS--AAASSSLRRFLLVGSLPID 840
             +PL  +T+ L G I FF+ WLA+FKLLLFA+GKGPL S        L  F+ + S PI 
Sbjct: 1443 VLPLNLRTIFLGGPISFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSHFITLASFPIK 1502

Query: 841  IYDHDPASTRPD-TNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLE 900
            I D+     +   TN  +      K   LS  I     K+  HPN  L ++   +Y+ LE
Sbjct: 1503 ILDNPINKQKKSLTNYAI------KALILSTIIPIQQNKSSIHPNVFLFLYSIYMYIALE 1562

Query: 901  IVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAA 960
            I+L   AA  +A L VEL P F+EPYLS+SLQDFWGRRWNLM S ILR +VY P R   +
Sbjct: 1563 IILATVAASVRAALCVELEPQFEEPYLSTSLQDFWGRRWNLMASAILRATVYVPVRTVTS 1622

Query: 961  AIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNA 1020
              +GRK A + A  A F VSG+MHELI++ +GRL PTWEVT FF +HG  LA E++VK A
Sbjct: 1623 RWIGRKWAPVPAVIAAFLVSGIMHELIFYNIGRLKPTWEVTWFFALHGICLAAEVSVKKA 1650

Query: 1021 VGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVA 1066
            V E++R P  V+  L + FVM T  WLF P   R + DV  + E  A   F+K+V+
Sbjct: 1683 VNEKWRLPPLVSGFLAVAFVMATGLWLFLPALMRLEADVMAYREMIAFIDFIKSVS 1650

BLAST of Cp4.1LG02g15830 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 461/718 (64.21%), Postives = 526/718 (73.26%), Query Frame = 0

Query: 367  MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 426
            ME+EIG  LKVWIS WICL YCYGVG +I  G TRFLFI PV+ +FLYLPL LHSLHLGG
Sbjct: 1    MEEEIGNFLKVWISTWICLSYCYGVGKRIPHGITRFLFILPVVSLFLYLPLLLHSLHLGG 60

Query: 427  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 486
            LTAFF+AWLANFKLLLF+AG GPL+  G+S+  FCA+ASFP+K+KQ P QK+SE A NG 
Sbjct: 61   LTAFFIAWLANFKLLLFSAGHGPLSAPGISLGTFCAIASFPVKLKQNPLQKASEFAINGE 120

Query: 487  AATVIDPKSNQN-HRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDL 546
                +  KS+ N H +SP +YAVKI LVAA+VKAY+YTHILPQKL W  LCFHIYF+L+L
Sbjct: 121  ----LPSKSDHNRHPQSPLSYAVKIFLVAAMVKAYNYTHILPQKLHWLFLCFHIYFLLEL 180

Query: 547  SFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATK 606
            S AAA++ V+ GGT+ELLPLFH PY STSLQDFWGRRWNL VTGILRP  + PA S A+K
Sbjct: 181  SLAAAASVVAFGGTVELLPLFHEPYFSTSLQDFWGRRWNLVVTGILRPAVFSPAASAASK 240

Query: 607  LIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKR 666
            L G RW P+ GV ATF+VSA MHELMFFYMSRQWPPPTWEV+AFFV HGGCL  EMV KR
Sbjct: 241  LFGSRWGPITGVTATFVVSAVMHELMFFYMSRQWPPPTWEVSAFFVFHGGCLVGEMVVKR 300

Query: 667  LWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAFSDSP 726
            +WI  W M PLP+ V+VPVTVGFFFW+CFWLFFPPFIN+CKADVRVV+EY +FG F    
Sbjct: 301  VWIQKWGMRPLPSIVTVPVTVGFFFWSCFWLFFPPFINSCKADVRVVEEYALFGLF---- 360

Query: 727  LMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQ 786
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 787  GAIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLLVGSLPIDIYDHDPASTRP-DT 846
               GFFIGWLASFKLLLFAFGKGPL S AASSSLRRFL +GSLPI+I    P  T P D 
Sbjct: 421  ---GFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEI----PQDTNPSDP 480

Query: 847  NSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATL 906
            N +LP +   KL  L L+  A+Y + Y HPN  LI FCF+IY LLEI++GGAAA  KATL
Sbjct: 481  NPILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATL 540

Query: 907  GVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAAAIVGRKPAAIMATT 966
            GVELLP FDEPYLS+SLQDFWG+RWNLM SRILRL+VYDPCR     IVG+KP+AI+A T
Sbjct: 541  GVELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMT 600

Query: 967  ATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNAVGERFRPPRFVTVP 1026
            ATFAVSGLMHELIYFY+GR+APTWEVT FFL+HG  L+ EMA+K+AVG RFR PR V+  
Sbjct: 601  ATFAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTG 643

Query: 1027 LTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVAAELITSVK-LISFQVK 1081
             T  FVM TS WLFFPQFFR KP+VRMFEEHAA+ AFVKNV  +LIT  K L+ F +K
Sbjct: 661  FTWWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 643

BLAST of Cp4.1LG02g15830 vs. ExPASy TrEMBL
Match: A0A7J6GAR9 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_010613 PE=3 SV=1)

HSP 1 Score: 823 bits (2127), Expect = 3.45e-275
Identity = 469/1067 (43.96%), Postives = 639/1067 (59.89%), Query Frame = 0

Query: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
            MEGEI N + VW+TV  SLCYCH  G ++  G  R + + P++++F +LPL L +I LGG
Sbjct: 1    MEGEINNLVMVWITVVASLCYCHTIGNIITSGTTRLIPILPVILIFLVLPLSLRTIFLGG 60

Query: 61   STSFFIAWLANFKLLLFAAGDGPLSAEQPL---PLRRFLALSCLPIKILQNEKSCPNNNR 120
              SFF+AWLANFKLLLFA G GPLS+  PL   PL RF+AL+  PIKI  +    P N +
Sbjct: 61   PISFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSRFIALASFPIKIQDD----PINKQ 120

Query: 121  SDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFI-HPKVVLFIYSWHLYFLLEIILAITQF 180
                   KS  NY+IKA+I + ++ +     +I +PKV L ++  ++Y  LEI+LA    
Sbjct: 121  K------KSLTNYAIKALIFSTIIPVQQNKSYIIYPKVFLSLHFIYIYTFLEIMLATVAA 180

Query: 181  FGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWA 240
              R  LG+ELEP F++PY STSLQDFWGRRWNLMA++ L+ TVY P   I ++ IGRKWA
Sbjct: 181  LVRATLGVELEPHFEEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRTIMSRWIGRKWA 240

Query: 241  PLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLP 300
            P+PAV+ TF+VS  +HE IFY +GR +P WE T FFV+HG+ L  EI VKKA+  KWRLP
Sbjct: 241  PVPAVIPTFMVSGFIHEFIFYNLGRSKPRWEVTLFFVVHGICLAAEIAVKKAINRKWRLP 300

Query: 301  PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKGIEKKKQS 360
            P V+  + LAF+  T +WLF P  +RL++DV+A  E    +  +K   + +        S
Sbjct: 301  PLVTGSMALAFVMSTVLWLFMPDLMRLEIDVKAHREMIEFLESIKMTRYQI-------GS 360

Query: 361  VIGRRSSGER--MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLY 420
             I    + E+  ME EI  L+ VWI+  + L YC+ +GN I  G TR + I PVI IFL 
Sbjct: 361  TIKAEVTREKILMESEINNLVMVWITAVVSLCYCHTIGNIITPGTTRLIPILPVILIFLV 420

Query: 421  LPLNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTG----MSIAEFCAVASFPIKI 480
            LPLNL ++ L     FFVAWLANFKLLLFA G+GPL++      + ++ F  +ASFPIKI
Sbjct: 421  LPLNLRTISLRVPILFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSRFITLASFPIKI 480

Query: 481  KQIPPQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKL 540
            +                    DP  N+  ++S  NYA+K L+++ ++        +   +
Sbjct: 481  QD-------------------DPIINKQ-KKSLTNYAIKALILSTIIPIQQNKSSIHPNV 540

Query: 541  VWFLLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGI 600
              F  C + Y IL++  A  +A V     +EL P F  PYLSTSLQDFWGRRWNL  +  
Sbjct: 541  FLFFYCIYNYIILEIILATVAALVRAALGVELEPNFEEPYLSTSLQDFWGRRWNLIASDS 600

Query: 601  LRPVAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFF 660
            LR   Y P  +  ++ IG +WAP+  V  TF+VS  +HE +F+ M R    PTWEV  FF
Sbjct: 601  LRSTVYIPVRTMMSRWIGRKWAPVPAVIPTFMVSGIIHEFIFYNMRRS-KQPTWEVTLFF 660

Query: 661  VLHGGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVR 720
            V+HG CLA E+  K+     W++PP+   VS  + V F   T  WLF P  +   +ADV 
Sbjct: 661  VVHGMCLAAEIAVKKAVNRKWRLPPV---VSGSLAVAFVMTTKLWLFMPA-LMALEADVN 720

Query: 721  VVKEYEIF--GAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPF 780
              +E   F      +  LM +EI   +   + V +SLC+C++I N I  G  + + ILP 
Sbjct: 721  AHREMIEFVESIKKEKILMESEINNLVMVWITVVASLCYCHTIGNIITPGTARLIAILPV 780

Query: 781  FLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLLVGSL 840
             L+FL +PL  +T+ L G I FF+ WLA+FKLLLFA+GKGPL S      L RF+ + S 
Sbjct: 781  ILIFLVLPLNLRTIILGGPISFFVAWLANFKLLLFAYGKGPLSSNPLPLPLSRFIPLASF 840

Query: 841  PIDIYDHDPASTRPDTNSVLPLSLSSKLFFLSLSIAAIYCKNY-FHPNGTLIVFCFVIYL 900
            PI I + DP + +  + +    + + K    S  I     K+Y  HP   + ++   IY+
Sbjct: 841  PIKIQE-DPLNKQKKSLT----NYAIKALIFSTIIPVQQNKSYTIHPKVFMSLYFIYIYI 900

Query: 901  LLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRN 960
             LEI+    AA+ +A LG EL P FDEPYLS+SLQDFWGRRWNLM S  LR +VY P RN
Sbjct: 901  FLEIIQAAVAALVRAALGAELEPHFDEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRN 960

Query: 961  FAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAV 1020
              +  +GRK A + A   TF +SG++HE I++ + R  PTWEVT  F+VHG  LA E+AV
Sbjct: 961  IMSRWIGRKWAPVPAVIPTFMMSGIIHEFIFYSMRRSKPTWEVTLLFVVHGMCLAAEIAV 1020

Query: 1021 KNAVGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEE 1054
            K AV  ++R P  V+V L + F+M T+ WLF P   R + DV+ + E
Sbjct: 1021 KKAVNRKWRLPPLVSVSLAVAFLMTTALWLFMPALMRLEVDVKAYSE 1020

BLAST of Cp4.1LG02g15830 vs. ExPASy TrEMBL
Match: A0A7J6E2P5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_009767 PE=3 SV=1)

HSP 1 Score: 818 bits (2114), Expect = 2.53e-268
Identity = 440/1037 (42.43%), Postives = 629/1037 (60.66%), Query Frame = 0

Query: 35   RFLAVAPIVVLFFLLPLQLTSIHLGGSTSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRR 94
            R     PIV LF  LPL L SI+L G T+FFI+WLANFKLLL A   GPL+ +       
Sbjct: 239  RLFLFLPIVSLFLFLPLNLYSINLCGGTAFFISWLANFKLLLLAFQKGPLALKNQ-SFGL 298

Query: 95   FLALSCLPIKILQNEKSCPNNNRSDTTLKPKSFLNYSIKAVIVALMVKLYD-YSEFIHPK 154
            F+A++CLPI+I +  K  P           +  +N+ IKA+++A++++  D Y + IHP 
Sbjct: 299  FIAIACLPIEI-KTTKETPK----------RQLINFPIKALLLAILIQFLDHYKQQIHPN 358

Query: 155  VVLFIYSWHLYFLLEIILAITQFFGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATN 214
            +VLF+Y + +Y LLEIILAIT    R V+G+E+EPQF +PY STSLQDFWGRRWNLM T 
Sbjct: 359  LVLFLYCFLIYLLLEIILAITAALARAVVGLEVEPQFNEPYLSTSLQDFWGRRWNLMVTR 418

Query: 215  ILQPTVYKPSVKIAAKVIGRKWAPLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFV 274
            IL+ TVY+P++ + + ++GRKWA +PA++ATF+VS ++HEL+++Y+ R+ P WE T FFV
Sbjct: 419  ILRHTVYQPAIGLISTLMGRKWASIPAIIATFLVSGLVHELMYFYLSRVPPTWEVTCFFV 478

Query: 275  IHGVALTVEIMVKKALPEKWRLPPAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEY 334
            +HG+ LTVEI++KKAL + WR P  +S  +T+ F+  T  WLFFPQF+R + DV+  +EY
Sbjct: 479  LHGICLTVEIILKKALGDTWRFPKVMSRMLTIGFVMLTGFWLFFPQFLRCKADVKLVQEY 538

Query: 335  AALVTFVKNVAFSLKGIEKKKQSVIGRRSSGERMEDEIGKLLKVWISIWICLGYCYGVGN 394
            A                                                          N
Sbjct: 539  A----------------------------------------------------------N 598

Query: 395  KIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATT 454
             + +G  R +   P++ +FL LPL L+S++LGG+TAFF++WLANFKLLLFA  +GPLA+ 
Sbjct: 599  FVSKGMKRLICFLPIVLLFLLLPLKLNSINLGGVTAFFISWLANFKLLLFAFEKGPLASN 658

Query: 455  GMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLV 514
              S+  F  +A  PI IK+ P                      Q  +    N+  KI+ +
Sbjct: 659  SNSLGLFVVIACLPIAIKENPSNNKM---------------IKQASKRHLINFPTKIIFL 718

Query: 515  AALVKAYDYTHILPQKLVWFLLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLST 574
              L++  DY   +   LV  + C  IY +L+   A A+        +EL P FH PYLST
Sbjct: 719  VVLLRVLDYKKQMHHNLVVLIYCPLIYLLLETILALAATLARALVRLELEPQFHEPYLST 778

Query: 575  SLQDFWGRRWNLAVTGILRPVAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFF 634
            SL DFWG RWNL +  ILRP  Y P ++  + L+G +WAP+  +  TF+VS  +HEL+++
Sbjct: 779  SLHDFWGCRWNLMIRRILRPTIYEPTLNLVSPLVGRKWAPIPAILGTFVVSGLVHELIYY 838

Query: 635  YMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTC 694
            Y++R    PTWEV +FF+LHG C  +E+  K+  ID W++P +   +S  +TVGF   T 
Sbjct: 839  YIARV--TPTWEVTSFFILHGLCYTIEICLKKTLIDTWQIPKV---ISRMLTVGFVTLTG 898

Query: 695  FWLFFPPFINTCKADVRVVKEYEIFGAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKN 754
             WLFFP F   CKADV++V+EY  F        M  E+  F++       SL +CY +  
Sbjct: 899  IWLFFPQFFR-CKADVKLVQEYAYFERKKGKEKMEGEMNNFIEVWFLALVSLSYCYVMSK 958

Query: 755  SIPNGFLKFLLILPFFLLFLYIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCSA 814
             I  G  + +  LP   LFLY+PL+  +++L G   FF+ WLA+FKLLL AF KGPL ++
Sbjct: 959  LISKGIKRLIFFLPIICLFLYLPLKLNSVNLVGTTSFFLSWLANFKLLLLAFNKGPLATS 1018

Query: 815  AASS-SLRRFLLVGSLPIDIYDHDPASTRPDTNSVLPL-SLSSKLFFLSLSIAAIYCKNY 874
              S  S  RF+++ S PI+I +++   ++  +N+ +P+ S + K   L++ I     K+ 
Sbjct: 1019 NPSPISFGRFIVIASFPIEIQENNTNPSKEKSNNKIPIISFTIKTLLLAILIRVCDYKDQ 1078

Query: 875  FHPNGTLIVFCFVIYLLLEIVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNL 934
             +PN  L+++CF++YLLLE +L   A + +   GV L P F+EPYL++SLQDFWG+RWNL
Sbjct: 1079 INPNVVLVLYCFLVYLLLETILVFVAIMVRVVAGVGLQPQFNEPYLTTSLQDFWGKRWNL 1138

Query: 935  MVSRILRLSVYDPCRNFAAAIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVT 994
            MV+RILR +V++P   F++  +GRK A + A  ATF VSGL+HELIYFY+ R  PTWEVT
Sbjct: 1139 MVTRILRPTVFEPTLKFSSTFIGRKWAQLPAIVATFVVSGLVHELIYFYMIRTPPTWEVT 1184

Query: 995  SFFLVHGFFLAVEMAVKNAVGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRM 1054
             FF++HG  L VE+ +K  +G+ +R P  V+  LTIGFVM+TSFWLFFPQ  R K DVR 
Sbjct: 1199 LFFVLHGLCLTVEIVLKKCLGDTWRLPSLVSGALTIGFVMLTSFWLFFPQLLRCKADVRT 1184

Query: 1055 FEEHAAMGAFVKNVAAE 1068
             EE+A +G F++ +  +
Sbjct: 1259 LEEYANLGKFLRKMVTQ 1184

BLAST of Cp4.1LG02g15830 vs. ExPASy TrEMBL
Match: A0A7J6HXG3 (Uncharacterized protein (Fragment) OS=Cannabis sativa OX=3483 GN=G4B88_022622 PE=3 SV=1)

HSP 1 Score: 801 bits (2069), Expect = 1.86e-264
Identity = 462/1076 (42.94%), Postives = 628/1076 (58.36%), Query Frame = 0

Query: 1    MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
            ++ EI N + VW+TV  SL YCH  G ++ PG  R + + P++++F +LPL L +I LGG
Sbjct: 663  IKSEINNLVMVWITVVASLYYCHTIGNIITPGTTRLIPILPVILVFLVLPLNLRTIFLGG 722

Query: 61   STSFFIAWLANFKLLLFAAGDGPLSAEQPL---PLRRFLALSCLPIKILQNEKSCPNNNR 120
              SFF+AWLANFKLLLF  G GPLS+  PL   PL  F+ L+  PIKIL N    P N +
Sbjct: 723  PISFFVAWLANFKLLLFVYGKGPLSSNNPLLPLPLSHFITLASFPIKILNN----PINKQ 782

Query: 121  SDTTLKPKSFLNYSIKAVIVALMVKLYDYSEFI-HPKVVLFIYSWHLYFLLEIILAITQF 180
                   KS  NY+IKA+I + ++ +     +I +PKV L IY  ++Y  LEI+LA    
Sbjct: 783  K------KSLTNYAIKALIFSTIIPVQQNKSYIIYPKVFLSIYFIYIYTFLEIMLATVAA 842

Query: 181  FGRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWA 240
              R  LG+ELEP F++PY STSLQDFWGRRWNLMA++ L+ TVY P   + ++ IGRKWA
Sbjct: 843  LVRAALGVELEPHFEEPYLSTSLQDFWGRRWNLMASDSLRSTVYIPVRTVMSRWIGRKWA 902

Query: 241  PLPAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLP 300
            P+PAV+ TF+VS ++HE IFY +GR +P WE T FFV+HG+ L  EI VKKA+  KWRLP
Sbjct: 903  PVPAVIPTFMVSGIIHEFIFYNLGRSKPRWEVTLFFVVHGICLAAEIAVKKAINRKWRLP 962

Query: 301  PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSLKGIEKKKQS 360
            P V+  + LAF+  T +WLF P  +RL++D++A                     E  ++ 
Sbjct: 963  PLVTGSMALAFVMSTVLWLFMPDLIRLEVDIKA---------------------EVTREK 1022

Query: 361  VIGRRSSGERMEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLP 420
            ++        ME E+  L+ VWI++   L YC+ +G  I  G  R + I PVI IFL LP
Sbjct: 1023 IL--------MESELNNLVMVWITVVASLCYCHTIGKIITPGTARLIAILPVILIFLVLP 1082

Query: 421  LNLHSLHLGGLTAFFVAWLANFKLLLFAAGQGPLATTGMSI--AEFCAVASFPIKIKQIP 480
            LNL ++ LGG  +FFVAWLANFKLLLFA G+GPL++  + +  + F  +ASFPIKI+  P
Sbjct: 1083 LNLRTIILGGPISFFVAWLANFKLLLFAYGKGPLSSNPLPLPLSRFIPLASFPIKIQDDP 1142

Query: 481  PQKSSEPAGNGGAATVIDPKSNQNHRESPFNYAVKILLVAALVKAY-DYTHILPQKLVWF 540
             +K                      ++S  NYA+K L+ + ++    + ++ +  K+   
Sbjct: 1143 LKKQ---------------------KKSLTNYAIKALIFSTIIPVQQNKSYSIHPKVFMS 1202

Query: 541  LLCFHIYFILDLSFAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRP 600
            L   +IY  L++  AA +A V      EL P F  PYLSTSLQDFWGRRWNL  +  LR 
Sbjct: 1203 LYFIYIYIFLEIIQAAVAALVRAALGAELEPHFDEPYLSTSLQDFWGRRWNLMASDSLRS 1262

Query: 601  VAYRPAVSTATKLIGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLH 660
              Y P  +  ++ IG +WAP+  V  TF++S  +HE +F+ M R    PTWEV   FV+H
Sbjct: 1263 TVYIPVRNIMSRWIGRKWAPVPAVIPTFMMSGIIHEFIFYSMRRS--KPTWEVTLLFVVH 1322

Query: 661  GGCLAVEMVAKRLWIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVK 720
            G CLA E+  K+     W++PPL   VS  + V F   T  WLF P  +   + DV+   
Sbjct: 1323 GMCLAAEIAVKKAVNRKWRLPPL---VSGSLAVAFLMTTALWLFMPALMRL-EVDVKAYS 1382

Query: 721  EYEIFGAFSDSPLMAAEILIFLQTLLQVSSSLCFCYSIKNSIPNGFLKFLLILPFFLLFL 780
            E                    +   + V +SLC+C++I   I  G  + + ILP  L+FL
Sbjct: 1383 EINNL----------------VMVWITVVASLCYCHTIGKIITPGTTRLIPILPVILVFL 1442

Query: 781  YIPLQFQTLHLQGAIGFFIGWLASFKLLLFAFGKGPLCS--AAASSSLRRFLLVGSLPID 840
             +PL  +T+ L G I FF+ WLA+FKLLLFA+GKGPL S        L  F+ + S PI 
Sbjct: 1443 VLPLNLRTIFLGGPISFFVAWLANFKLLLFAYGKGPLSSNNPLLPLPLSHFITLASFPIK 1502

Query: 841  IYDHDPASTRPD-TNSVLPLSLSSKLFFLSLSIAAIYCKNYFHPNGTLIVFCFVIYLLLE 900
            I D+     +   TN  +      K   LS  I     K+  HPN  L ++   +Y+ LE
Sbjct: 1503 ILDNPINKQKKSLTNYAI------KALILSTIIPIQQNKSSIHPNVFLFLYSIYMYIALE 1562

Query: 901  IVLGGAAAVAKATLGVELLPCFDEPYLSSSLQDFWGRRWNLMVSRILRLSVYDPCRNFAA 960
            I+L   AA  +A L VEL P F+EPYLS+SLQDFWGRRWNLM S ILR +VY P R   +
Sbjct: 1563 IILATVAASVRAALCVELEPQFEEPYLSTSLQDFWGRRWNLMASAILRATVYVPVRTVTS 1622

Query: 961  AIVGRKPAAIMATTATFAVSGLMHELIYFYLGRLAPTWEVTSFFLVHGFFLAVEMAVKNA 1020
              +GRK A + A  A F VSG+MHELI++ +GRL PTWEVT FF +HG  LA E++VK A
Sbjct: 1623 RWIGRKWAPVPAVIAAFLVSGIMHELIFYNIGRLKPTWEVTWFFALHGICLAAEVSVKKA 1650

Query: 1021 VGERFRPPRFVTVPLTIGFVMVTSFWLFFPQFFRFKPDVRMFEEHAAMGAFVKNVA 1066
            V E++R P  V+  L + FVM T  WLF P   R + DV  + E  A   F+K+V+
Sbjct: 1683 VNEKWRLPPLVSGFLAVAFVMATGLWLFLPALMRLEADVMAYREMIAFIDFIKSVS 1650

BLAST of Cp4.1LG02g15830 vs. ExPASy TrEMBL
Match: A0A6J1K824 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493084 PE=3 SV=1)

HSP 1 Score: 714 bits (1844), Expect = 1.41e-248
Identity = 347/355 (97.75%), Postives = 347/355 (97.75%), Query Frame = 0

Query: 367 MEDEIGKLLKVWISIWICLGYCYGVGNKIGEGFTRFLFIFPVICIFLYLPLNLHSLHLGG 426
           MEDEIG LLKVWISIWICLGYCYGVG KIGEGFTRFL IFPVICIFLYLPL LHSLHLGG
Sbjct: 1   MEDEIGNLLKVWISIWICLGYCYGVGKKIGEGFTRFLLIFPVICIFLYLPLKLHSLHLGG 60

Query: 427 LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQIPPQKSSEPAGNGG 486
           LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQI PQKSSEPAGNGG
Sbjct: 61  LTAFFVAWLANFKLLLFAAGQGPLATTGMSIAEFCAVASFPIKIKQILPQKSSEPAGNGG 120

Query: 487 AATVIDPKSNQNHRESPFNYAVKILLVAALVKAYDYTHILPQKLVWFLLCFHIYFILDLS 546
           AATVIDPKSNQNH ESPFNYAVKILLVAALVKAYDYTHIL QKLVWFLLCFHIYFILDLS
Sbjct: 121 AATVIDPKSNQNHCESPFNYAVKILLVAALVKAYDYTHILHQKLVWFLLCFHIYFILDLS 180

Query: 547 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 606
           FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL
Sbjct: 181 FAAASAAVSHGGTIELLPLFHHPYLSTSLQDFWGRRWNLAVTGILRPVAYRPAVSTATKL 240

Query: 607 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 666
           IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL
Sbjct: 241 IGPRWAPLAGVAATFLVSAAMHELMFFYMSRQWPPPTWEVAAFFVLHGGCLAVEMVAKRL 300

Query: 667 WIDAWKMPPLPAAVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF 721
           WIDAWKMPPLPA VSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF
Sbjct: 301 WIDAWKMPPLPAVVSVPVTVGFFFWTCFWLFFPPFINTCKADVRVVKEYEIFGAF 355

BLAST of Cp4.1LG02g15830 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 332.0 bits (850), Expect = 1.8e-90
Identity = 173/338 (51.18%), Postives = 232/338 (68.64%), Query Frame = 0

Query: 7   NFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGGSTSFFI 66
           +FIK W  V ISLCY     KL+P G  R +   P+ ++FF++P  + S+HL G T+FFI
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 67  AWLANFKLLLFAAGDGPLSA-EQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDTTLKPK 126
           AWLANFKLLLFA G GPLS+  +PL L  FLA+SCLPIKI  + K    ++   +T  P 
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGP- 122

Query: 127 SFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRNVLGIE 186
             L Y+IKAV V L++K Y+YS  +  KVVL +Y+ H+YF LEIILA T    R +  +E
Sbjct: 123 --LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 182

Query: 187 LEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPAVMATF 246
           LEPQF  PY +TSLQDFWGRRWNLM T IL+PTVY+PS+++ + V+G  ++ + A   TF
Sbjct: 183 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTF 242

Query: 247 IVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKALPEKWRLPPAVSVPVTL 306
           +VS +MHELIF+YMGR+RP+W+  WFF+I+G   TVEI +KK +  +WR P A+S  +TL
Sbjct: 243 VVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTL 302

Query: 307 AFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNV 344
            F+  TA+WLF P+F R  +  +A +EYAA+  F   V
Sbjct: 303 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

BLAST of Cp4.1LG02g15830 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 271.9 bits (694), Expect = 2.2e-72
Identity = 151/345 (43.77%), Postives = 209/345 (60.58%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           M+ E+ N IKVW++  IS+ YC+     +  GA RFL+V+P++ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLR--RFLALSCLPIKILQNEKSCPNNNRS 120
            T+FF+ WLANFKL+LF+   GPL    P+P    RF   +C PIK+ QN KS       
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKS------- 120

Query: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180
                PK  L ++IK  I A+++ LY Y + + P ++L +Y  HLY  +EIIL   +   
Sbjct: 121 -QNHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKK--ALPEKWRLP 300
           P ++A FIVS ++HEL+F+Y+ R  P  E T FFV+HGV   VE+ VKK   + ++WRL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVK 342
           P VS  +T+ F+F T  WLF PQ  R  +  R   E   LV F+K
Sbjct: 301 PGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Cp4.1LG02g15830 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 256.9 bits (655), Expect = 7.2e-68
Identity = 144/348 (41.38%), Postives = 204/348 (58.62%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           ME E+++ IKVW+   IS+ YC+ T   +  G  R L+V P+ VLF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDT 120
           ST+FF++WLANFKL+LF+   GPL    P  L RF+  +C PIK+ QN K  P N     
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPL-FPVPSNLSRFVCFTCFPIKLQQNPK--PQNQ---- 120

Query: 121 TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRN 180
              PK    +++K     +++ +Y+Y + + P V+L +YS H+Y   EI+LA  +     
Sbjct: 121 --MPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSI 180

Query: 181 VLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPA 240
            L  +LEP F +PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A    
Sbjct: 181 SLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLG 240

Query: 241 VMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA-LPEKWRLPPAV 300
           V A+F+VS ++HELIF+Y  R  P  E T FFV+HGV    E   K+  L  +W++   V
Sbjct: 241 VFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMV 300

Query: 301 SVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKNVAFSL 348
           S  +T+ F+  T  WLFFP   R  +  R  +E    + FVK+  F L
Sbjct: 301 SRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

BLAST of Cp4.1LG02g15830 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 250.4 bits (638), Expect = 6.7e-66
Identity = 136/346 (39.31%), Postives = 205/346 (59.25%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           ME E+ NFIK+W++  IS+ YC+     +  G  R L+V P+  LF + PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLP--LRRFLALSCLPIKILQNEKSCPNNNRS 120
             +FF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK        P  N +
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIK--------PQQNPN 120

Query: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180
               K   +L ++IK VI  +++++Y+Y +++ P ++L   S H++  LEI+  + +   
Sbjct: 121 IQNYKIPIWL-FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALV 180

Query: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240
              LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+P VY P+ ++A + +    A  
Sbjct: 181 FITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMF 240

Query: 241 PAVMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA--LPEKWRLP 300
             V A F+VS  +HE++F+Y+ R  P  E TWFF++HGV    E+ VKK+  +   WR+ 
Sbjct: 241 LGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVS 300

Query: 301 PAVSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVKN 343
           P VS  +T+ F+  T+ W FFP  +R  +  R   E    + FVK+
Sbjct: 301 PTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVKH 333

BLAST of Cp4.1LG02g15830 vs. TAIR 10
Match: AT1G34500.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 235.3 bits (599), Expect = 2.2e-61
Identity = 132/343 (38.48%), Postives = 196/343 (57.14%), Query Frame = 0

Query: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLPPGAGRFLAVAPIVVLFFLLPLQLTSIHLGG 60
           ME E+ NFI VW++  IS+ YC+     +  G  R  +V PI  LFF+LPL  +S+H   
Sbjct: 1   MEEELKNFIIVWISAIISVSYCYYISANIKTGVLRLFSVLPICGLFFVLPLFFSSVHFSS 60

Query: 61  STSFFIAWLANFKLLLFAAGDGPLSAEQPLPLRRFLALSCLPIKILQNEKSCPNNNRSDT 120
           ST+F+++ +A+ KL+LFA   GPL    P  L +F+  +C PIK+ +N KS P+ N    
Sbjct: 61  STAFYLSEMASLKLILFAFDQGPLFPVAP-NLIQFVCFTCFPIKLQRNPKSQPSQNHFHK 120

Query: 121 TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFGRN 180
                    ++IK +I  +++ +Y+YS F+   V+L +   HLY  LEI+L   +     
Sbjct: 121 RA-------FAIKIMIFGVVLHVYNYSHFLPQTVLLSLCFLHLYVELEILLGPLKVLLSM 180

Query: 181 VLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPLPA 240
            LG +LEPQF  PY +TSLQDFWGRRWNLM +++L+  +Y P      + +   WA    
Sbjct: 181 ALGCDLEPQFNKPYLATSLQDFWGRRWNLMVSSVLRSGIYNPVRCACQRPMNSGWARFMG 240

Query: 241 VMATFIVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKA--LPEKWRLPPA 300
            + TF+VS + HEL+++Y+ R  P WE T FFV++GV    E+ VK+   L   W +  +
Sbjct: 241 YLVTFLVSGLFHELVYFYITRETPTWEVTLFFVLNGVCTGTEVAVKRTAFLQRWWPVRSS 300

Query: 301 VSVPVTLAFIFPTAIWLFFPQFVRLQLDVRAFEEYAALVTFVK 342
           VS  +T+ F+  T   LFFP F+R  +  R   E    + FVK
Sbjct: 301 VSRLLTMGFVVVTGGLLFFPLFIRSGMMERRANETLFFLDFVK 335