Cp4.1LG02g13280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g13280
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	GTP cyclohydrolase II
Location	Cp4.1LG02: 11224624 .. 11227747 (-)
RNA-Seq Expression	Cp4.1LG02g13280
Synteny	Cp4.1LG02g13280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAATCGCCCTGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCTAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGACGGCTACTCTTCCATTCAGACCGCCGTTGATGCTATTAGCCAAGGAAAGGTTGCCATATGTCCATTTCCTTATGGATAATACTTACTTACACGCTAATTCTTTTTTCTATCTTATCCATTTCTCTTTCCATTTGGAACGCTCAACTCCACCGTTAGTATTTTTTTTTCGGGTTTCTCCTCAAAGTTTTTTAAAAGCATATGGTAGTGAGAGGTTTTCACATCCTTACGAATGGTGTTTTGTTCTCCGGTGTGAGATCTCACAATCCACCCCCTTCAGGATACAACGTCGGTTCAATATCTGTCTCTGATATCATTTATAACAACCCAGACCCACCACTAGTAGATATTGTTTTTTTCGAGTTTTCTCTTAAAATTTTTAAAACCTATCAGTTAAAATGTTTCGTTCTCTTCCCGAACCTACCTGTCATCTTACAAGGTTACAAAGGAACAAAAATTATTTTCTCTTTGTACTTATTAGTTGAGGATGTGGCATGTTTGTATGGGTCTTTCTTATTAATTTTCTTTTTCTTCTCTTACCCTTTTTGCATGCATTTCTCTTCTCTCTCCTCATGCATTTCTCTCTCCTAATGGCCAAAAAACTTGATTGTTTTCTAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCCATCGATAATGGGAACGAGACATCAACTTTTACTATCACTGTGGTACGTAGTACGCATGAGAAATTTGAACTTCTGACCTCTTGATTAAATTATTATACATGACCGCACCCAGGTAGGGTTTATTACTTTGAGTGTTACTAATTTATTGTTTGGATGAGACAGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCACCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCTCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACAGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGATGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGGTAAGTCAAGTCGATTGGTCGATAATTGATGTCTTATGCATTGAACTATAAAAAATTTGAGTCGGGTTTGGATTCACTTAATTTTTTTTGGCTGTTTATTTTGGTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGGATTGAGCACGTGGCTCTTGTAAAGGTAATGGACTTTGTTTTCTTTGAGGAATAATGCATTCATTAACACATGACATCCTTAAATATCAAAAACATATGATAGTTGGTGGTCGATGAGCTTCAAGATCCAATATTTAACCTAAAAAATAATAATATTTGCTTGAGATTAGTTAAATCTTTAGTGACATCCAAAATCTGAAGATATTCCGTATTGAATTATGATTTACCGTATATATCATATTTGAATTTTTATTATATGCAAATATCTTGTATTGCAACTTTTTTTTTTTTTATAATTTATTTGATTCTGTAAATAATTATAAATAGAAAACTTTCGCCACGATTTATTTCAACAGGGAAACATTGGGGACGGTCAAGATGTGCTTGTAAGGGTCCATTCCGAATGTTTAACTGGCGACATATTCGCGTCGGCAAGATGCGACTGTGGCAACCAACTGGACTTAGCAATGCAAATGATTGAGAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACTTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGGTCAGTCAACTCAACTCGACTCGAGTTAACTAAAGTTAAATTTGATTTTGTTATTTTATTTGTGGATGATGTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAACAAATTGAATTTATCCCTTCAACAAATAAATAAATAATTAAATAAATAAATAATTAAATAAATAAATGAATGGATGCATAATTAGATAAATAAAATTTAATCAAATAAAAGGGATGGTCGAGTATTTGTTGCTCGCAATGCCCCATTGCTTGTTGTTGTCTCCTTGCCTTTGGCTTCATTCTAATAATAAAGGATTGGTGGTGGTTGTTTTTTGTGTGTTAATTGGTATGCCAAACTTGTAACCTCTTATCTTCCTCATGATCCATCGAGGTGATCGTCGGCGTCTTTATCTGTGTCGTTGGCTCTTAGAATCACGACCGACGGTGTCGAGTTCTTCCTCTGATCGATTGAAGCAAAATTCAATCGCTTTGTTTTCTAGCAAAATTGTGGAGAGGGTTCAACAAAATAGTAAGGGTGAATTGGTAATAAGGGCAAGAATTATTGTATGGGATGAAAGCAGCAA

mRNA sequence

TTTAATCGCCCTGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCTAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGACGGCTACTCTTCCATTCAGACCGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCCATCGATAATGGGAACGAGACATCAACTTTTACTATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCACCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCTCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACAGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGATGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGGATTGAGCACGTGGCTCTTGTAAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACTTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAACAAATTGAATTTATCCCTTCAACAAATAAATAAATAATTAAATAAATAAATAATTAAATAAATAAATGAATGGATGCATAATTAGATAAATAAAATTTAATCAAATAAAAGGGATGGTCGAGTATTTGTTGCTCGCAATGCCCCATTGCTTGTTGTTGTCTCCTTGCCTTTGGCTTCATTCTAATAATAAAGGATTGGTGGTGGTTGTTTTTTGTGTGTTAATTGGTATGCCAAACTTGTAACCTCTTATCTTCCTCATGATCCATCGAGGTGATCGTCGGCGTCTTTATCTGTGTCGTTGGCTCTTAGAATCACGACCGACGGTGTCGAGTTCTTCCTCTGATCGATTGAAGCAAAATTCAATCGCTTTGTTTTCTAGCAAAATTGTGGAGAGGGTTCAACAAAATAGTAAGGGTGAATTGGTAATAAGGGCAAGAATTATTGTATGGGATGAAAGCAGCAA

Coding sequence (CDS)

TTTAATCGCCCTGCTTTGCCTCATCCTAAGATTGGCAAATGTAAGCTAAGAGGGCTGCGTTTTGTTTGCTCCGCTGCTGCTGACTTGTTTGATGACAACCTCGCCGCTAAAAATGGTAACGGTGCTGCTGCTGCTGCTGCCGCCGCTGCTTTAGGACTTGAGGCTGAAACCACGCCCGATGTTGTTGATTTCTTTGATGATGAGGACGATCCTGACTGCCCATCTGACGGCTACTCTTCCATTCAGACCGCCGTTGATGCTATTAGCCAAGGAAAGTTTGTGATCGTTGTGGATGATGAAAATGAGGACACGGAAGGGAATTTGGTAATGGCTGCCTCTTTTACAACTCCTAGTAATATAACAACCATGGTTCAACATGGCTCAGGCATACTCTATGTGGGTATGAAAGAGGACGATCTCGAAAGGCTTAAGCTTCCCATAATATCCATCGATAATGGGAACGAGACATCAACTTTTACTATCACTGTGGATTTGAAAAATGGGACATCAGCAGGAGTATCAACTGCAGATAGAGCTAAGACAATTGTTGCTTTATCATCACCTGAGTCTAAGGCTGAGGACTTCAGACGGCCAGGTCACGTGTTTCCTCTCCGGTACCGAGACGGCGGCGTTCTGAGGAGAGCTGGCCACACAGAAGCTTCTGTAGATTTAGTTTCCCTTGCTGGGTTAAGGCCAGTTTCTGTTCTTTCAACCATTGTTGACCCTGAAGATGGCTCCATGGCTTCACTACCCAAGATGAGAAAGTTGGCCTCAGAAAAGAATATCCCAATAGTCTCTATTACTGATTTGATTAGATACCGAAGAAAGAGAGAAAATTTGATTGAAAGAATTGCTATTTCTCGGCTGCCAACTAAATGGGGCCAATTCTATGCTCACTGCTATCGCTCAAATCTAGATGGGATTGAGCACGTGGCTCTTGTAAAGGCTGGAAGAGGCGCTGTTATTTACCTAAGAGGTCATGAAGGAAGAGGCATTGGCCTTGGCCCCAAGCTGCTTGCCTACAACTTACAAGATCAAGGCCACGACACAGTCGAAGCCAACATCGAGCTAGGATTGCCTGCCGACGCCCGTGATTATGGAGTCGGAGCTCAGATGCTGAGAGATATTGGGATTAGAACAATGCGGTTGATGACAAACAACCCCGCGAAGTTCAAGGGGCTGAAGGGATATGGTTTGGCAGTGACAGCGAGGGTCCCTGTGGTGACACCCATTACAGATGAAAACAGAAAGTATTTGGAAACCAAGAGAACCAAGATGGGCCATATCTATGCGAAGGGCCAATTTTTAAGGTCGCGACAGGCTGAAGCTTCAACAAATTGA

Protein sequence

FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLRSRQAEASTN

Homology

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot
Match: Q9FN89 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA3 PE=1 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 5.7e-149
Identity = 277/451 (61.42%), Postives = 337/451 (74.72%), Query Frame = 0


Query: 28  DLFDDNLAAKNGNGAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSDGYSSIQTAV 87
           ++FD+N   K       +A+     ++AE TP+  DFF  D E DPDCP+ GYSSI+ A+
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET--VDAEITPETDDFFVSDAEGDPDCPTQGYSSIELAL 110

Query: 88  DAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKL 147
            A+ +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL L
Sbjct: 111 QALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSL 170

Query: 148 PIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVF 207
            ++S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS ++K +DFRRPGHVF
Sbjct: 171 TLMSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVF 230

Query: 208 PLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNI 267
           PL+YRDGGVLRRAGHTEASVDL+ LAGLRP+SVLS I+D EDGSMASLP M+KLA+E +I
Sbjct: 231 PLKYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDI 290

Query: 268 PIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKA------ 327
           PIVS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVK       
Sbjct: 291 PIVSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGE 350

Query: 328 -------------------------------------GRGAVIYLRGHEGRGIGLGPKLL 387
                                                GRG V+YLRGHEGRGIGLG KL 
Sbjct: 351 DILVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLR 410

Query: 388 AYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGL 431
           AYNLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNPAKF GLKGYGL
Sbjct: 411 AYNLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGL 470

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot
Match: Q6L506 (Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA3 PE=2 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 1.3e-137
Identity = 263/472 (55.72%), Postives = 332/472 (70.34%), Query Frame = 0


Query: 12  GKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAA----AAAAAALGLEAETTPDVVDFF-- 71
           G  KLR          +  +D L   N NG        ++A+   + A+ T +  DFF  
Sbjct: 38  GSLKLRCYAIGGLGGGENLNDPLKESN-NGPVLQGFNGSSASFRTVGAKITQETGDFFVS 97

Query: 72  DDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQ 131
           D E DPD P+DG+SSI  A+ A+ +GKFVI VDDE+ D EG+LVMAA+   P +I  M++
Sbjct: 98  DAEGDPDKPTDGFSSIDEAIGALHEGKFVIAVDDESGDNEGDLVMAATLADPESIAFMIR 157

Query: 132 HGSGILYVGMKEDDLERLKLPIIS----IDNGNETSTFTITVDLKNGTSAGVSTADRAKT 191
           +GSGI+ VGMKE+DL RL +P++S    I++ +  ++ T+TVD + G S GVS ADRAKT
Sbjct: 158 NGSGIISVGMKEEDLTRLMIPMMSPIAEIEDISAAAS-TVTVDARVGISTGVSAADRAKT 217

Query: 192 IVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVD 251
           I  L+SP+SK  D RRPGH+FPL+YR+GGVL+RAGHTEASVDLV+LAGLRPVSVLST+++
Sbjct: 218 IFTLASPDSKPTDLRRPGHIFPLKYRNGGVLKRAGHTEASVDLVALAGLRPVSVLSTVIN 277

Query: 252 PEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCY 311
           P DGSMA +P ++++A E +IPIVSI DLIRYRRKRE L+E IA+SRLPTKWG F A+CY
Sbjct: 278 PVDGSMAGMPVLKQMALEHDIPIVSIADLIRYRRKREKLVELIAVSRLPTKWGLFRAYCY 337

Query: 312 RSNLDGIEHVALV-------------------------------------------KAGR 371
           +S LDG EH+A+                                            KAGR
Sbjct: 338 QSKLDGTEHIAVAKGDIGDGEDVLVRVHSECLTGDILGSARCDCGNQLDLAMQLIDKAGR 397

Query: 372 GAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGI 431
           G ++YLRGHEGRGIGLG KL AYNLQD GHDTV+AN+ELGL  D+R+YG+GAQ+LRD+G+
Sbjct: 398 GVLVYLRGHEGRGIGLGQKLRAYNLQDDGHDTVQANVELGLAVDSREYGIGAQILRDMGV 457

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot
Match: Q6Z234 (Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA1 PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 2.6e-133
Identity = 245/434 (56.45%), Postives = 309/434 (71.20%), Query Frame = 0


Query: 46  AAAAALGLEAETTPDVVDFF---DDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENE 105
           A AAALG  A     VVD F   DDE D D P++G+SSI  A++ I QGK+VIVVDDE+ 
Sbjct: 75  ADAAALGTIAADMAPVVDGFSADDDELDLDSPTEGFSSIPEAIEDIRQGKYVIVVDDEDR 134

Query: 106 DTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTF 165
           + EG+L+MAAS  TP  +  +V+HG+GI+ V MKEDDLERL+LP++     NE    + F
Sbjct: 135 ENEGDLIMAASKVTPEAMAFIVRHGTGIVCVSMKEDDLERLELPLMVTTKENEEKLRTAF 194

Query: 166 TITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTE 225
           T++VD K GT+ GVS  DRA T++AL+SP SK EDF RPGH+FPL+YR+GGVL+RAGHTE
Sbjct: 195 TVSVDAKEGTTTGVSAKDRANTVLALASPNSKPEDFNRPGHIFPLKYREGGVLKRAGHTE 254

Query: 226 ASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKREN 285
           ASVDL  LAGL P +VL  IVD +DGSMA LPK++  A  +N+ I+SI DLIRYRRKR+ 
Sbjct: 255 ASVDLAMLAGLPPAAVLCEIVDDDDGSMALLPKLQDFARRENLKIISIADLIRYRRKRDR 314

Query: 286 LIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALV------------------------- 345
           L+ER+ ++ L  +WG F ++CYRS +DG+EH+A+V                         
Sbjct: 315 LVERVCVTPLQLQWGSFQSYCYRSLIDGMEHIAMVKGDVGDGQDILVRVHSECLTGDIFG 374

Query: 346 ------------------KAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIE 405
                             K GRG V+YLRGHEGRGIGLG KL AYNLQD G DTVEAN +
Sbjct: 375 SARCDCGNQLALAMTMIEKTGRGVVVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANED 434

Query: 406 LGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENR 431
           LGLP D+R+YG+GAQ+LRD+G+RTMRLMTNNPAK+ GLKGYGL+V  RVP++TPIT+ENR
Sbjct: 435 LGLPVDSREYGIGAQILRDLGVRTMRLMTNNPAKYTGLKGYGLSVLGRVPLLTPITNENR 494

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot
Match: Q0E079 (Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBA2 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.3e-132
Identity = 245/427 (57.38%), Postives = 304/427 (71.19%), Query Frame = 0


Query: 61  VVDFFDDED-----DPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFT 120
           V D F DED     D D P++G+SSI  A+  I QGK VIVVDDE+ + EG+L+MAAS  
Sbjct: 114 VADMFSDEDKDTEQDLDSPTEGFSSISEAIKDIQQGKLVIVVDDESRENEGDLIMAASLV 173

Query: 121 TPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAG 180
           TP  +  +V++G+GI+ V MKE+DLERL LP++     NE    + FT+TVD K GT+ G
Sbjct: 174 TPEAMAFVVRYGTGIVCVSMKEEDLERLNLPLMVATKENEEKLCTAFTVTVDAKEGTTTG 233

Query: 181 VSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRP 240
           VS  DRAKT++ L+SP+SK EDF RPGH+FPL+YR+GGVL+RAGHTEASVDL  LAGL P
Sbjct: 234 VSAKDRAKTVMTLASPDSKPEDFNRPGHIFPLKYREGGVLKRAGHTEASVDLAMLAGLPP 293

Query: 241 VSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTK 300
            +VL  IVD EDGSMA LPK+R  A  +N+ IVSI DLIRYRRKR+ L+ER +++RLP +
Sbjct: 294 AAVLCEIVD-EDGSMARLPKLRVFAERENLKIVSIADLIRYRRKRDRLVERSSVARLPLR 353

Query: 301 WGQFYAHCYRSNLDGIEHVALV-------------------------------------- 360
           WG   A+CYRS +DGIEH+A+V                                      
Sbjct: 354 WGNVRAYCYRSVIDGIEHIAMVKGEIGDGQGVLVRVHSECLTGDIFGSARCDCGDQLAMA 413

Query: 361 -----KAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVG 420
                KAGRG ++YLRGHEGRGIGLG KL AYNLQD G DTVEAN +LGLP D+R+YG+G
Sbjct: 414 MEMIEKAGRGVLVYLRGHEGRGIGLGHKLRAYNLQDDGRDTVEANEDLGLPVDSREYGIG 473

Query: 421 AQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHI 435
           AQ+LRD+G+R+M+LMTNNPAK+ GLKGYGL++  RVP+VTPIT ENR+YLETKRTKMGH+
Sbjct: 474 AQILRDLGVRSMKLMTNNPAKYGGLKGYGLSIVGRVPLVTPITSENRRYLETKRTKMGHV 533

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot
Match: P47924 (Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBA1 PE=1 SV=2)

HSP 1 Score: 464.5 bits (1194), Expect = 1.3e-129
Identity = 248/456 (54.39%), Postives = 316/456 (69.30%), Query Frame = 0


Query: 39  GNGAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVD 98
           G  AA +A A A G  AE         DD+ + D P+ G+SSI  A++ I QGK V+VVD
Sbjct: 98  GTLAADSAPAPANGFVAE---------DDDFELDLPTPGFSSIPEAIEDIRQGKLVVVVD 157

Query: 99  DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE--- 158
           DE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDDLERL LP++     NE   
Sbjct: 158 DEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHLPLMVNQKENEEKL 217

Query: 159 TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRA 218
           ++ FT+TVD K+GT+ GVS  DRA TI++L+S +SK EDF RPGH+FPL+YR+GGVL+RA
Sbjct: 218 STAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFNRPGHIFPLKYREGGVLKRA 277

Query: 219 GHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRR 278
           GHTEASVDL  LAGL PV VL  IVD +DGSMA LPK+R+ A+E N+ +VSI DLIRYRR
Sbjct: 278 GHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREFAAENNLKVVSIADLIRYRR 337

Query: 279 KRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVK-------------------- 338
           KR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VK                    
Sbjct: 338 KRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKGEIGDGQDILVRVHSECLTG 397

Query: 339 -----------------------AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVE 398
                                   GRG ++YLRGHEGRGIGLG KL AYNLQD G DTVE
Sbjct: 398 DIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVE 457

Query: 399 ANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPIT 446
           AN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNPAK+ GLKGYGLA+  RVP+++ IT
Sbjct: 458 ANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYGLAIVGRVPLLSLIT 517

[Close]

BLAST of Cp4.1LG02g13280 vs. NCBI nr
Match: XP_023522983.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 849 bits (2194), Expect = 5.16e-309
Identity = 446/489 (91.21%), Postives = 446/489 (91.21%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPD 60
           FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPD
Sbjct: 22  FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPD 81

Query: 61  VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 120
           VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI
Sbjct: 82  VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 141

Query: 121 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 180
           TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK
Sbjct: 142 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 201

Query: 181 TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV 240
           TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV
Sbjct: 202 TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV 261

Query: 241 DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 300
           DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC
Sbjct: 262 DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 321

Query: 301 YRSNLDGIEHVALVK-------------------------------------------AG 360
           YRSNLDGIEHVALVK                                           AG
Sbjct: 322 YRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAG 381

Query: 361 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 420
           RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG
Sbjct: 382 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 441

Query: 421 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 446
           IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR
Sbjct: 442 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 501

[Close]

BLAST of Cp4.1LG02g13280 vs. NCBI nr
Match: XP_022948312.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 840 bits (2170), Expect = 2.42e-305
Identity = 443/490 (90.41%), Postives = 445/490 (90.82%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA-LGLEAETTP 60
           FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA LGLEAETTP
Sbjct: 22  FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAAALGLEAETTP 81

Query: 61  DVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN 120
           DVVDFFDDEDDPDCPS+GYSSIQ AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN
Sbjct: 82  DVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN 141

Query: 121 ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA 180
           ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA
Sbjct: 142 ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA 201

Query: 181 KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI 240
           KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI
Sbjct: 202 KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI 261

Query: 241 VDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH 300
           VDPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH
Sbjct: 262 VDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH 321

Query: 301 CYRSNLDGIEHVALVK-------------------------------------------A 360
           CYRSNLDGIEHVALVK                                           A
Sbjct: 322 CYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKA 381

Query: 361 GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI 420
           GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI
Sbjct: 382 GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI 441

Query: 421 GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL 446
           GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL
Sbjct: 442 GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL 501

[Close]

BLAST of Cp4.1LG02g13280 vs. NCBI nr
Match: KAG6607228.1 (Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 831 bits (2147), Expect = 8.02e-302
Identity = 439/491 (89.41%), Postives = 443/491 (90.22%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA--LGLEAETT 60
           FNR +LPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA  LGLEAETT
Sbjct: 22  FNRLSLPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAAAALGLEAETT 81

Query: 61  PDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPS 120
           PDVVDFFDDEDDPDCPS+GYSSIQ AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPS
Sbjct: 82  PDVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPS 141

Query: 121 NITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADR 180
           NITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADR
Sbjct: 142 NITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADR 201

Query: 181 AKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLST 240
           AKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLST
Sbjct: 202 AKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLST 261

Query: 241 IVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYA 300
           IVDPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYA
Sbjct: 262 IVDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYA 321

Query: 301 HCYRSNLDGIEHVALVK------------------------------------------- 360
           HCYRSNLDGIEHVALVK                                           
Sbjct: 322 HCYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEK 381

Query: 361 AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRD 420
           AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRD
Sbjct: 382 AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRD 441

Query: 421 IGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF 446
           IGIRTMRLMTNNP+KFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF
Sbjct: 442 IGIRTMRLMTNNPSKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF 501

[Close]

BLAST of Cp4.1LG02g13280 vs. NCBI nr
Match: XP_022998776.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 823 bits (2127), Expect = 7.40e-299
Identity = 434/489 (88.75%), Postives = 439/489 (89.78%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPD 60
           F+RPALPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNGNGAA+AAA   LGLEAETTPD
Sbjct: 22  FHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAASAAA---LGLEAETTPD 81

Query: 61  VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 120
           VVDFFDDEDDPDCPS+GYSSI TAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI
Sbjct: 82  VVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 141

Query: 121 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 180
           TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK
Sbjct: 142 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 201

Query: 181 TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV 240
           TIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLR VSVLSTIV
Sbjct: 202 TIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIV 261

Query: 241 DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 300
           DPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC
Sbjct: 262 DPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 321

Query: 301 YRSNLDGIEHVALVK-------------------------------------------AG 360
           YRSNLDGIEHVALVK                                           AG
Sbjct: 322 YRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAG 381

Query: 361 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 420
           RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG
Sbjct: 382 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 441

Query: 421 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 446
           IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR
Sbjct: 442 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 501

[Close]

BLAST of Cp4.1LG02g13280 vs. NCBI nr
Match: XP_022159223.1 (monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momordica charantia])

HSP 1 Score: 659 bits (1701), Expect = 8.45e-234
Identity = 356/483 (73.71%), Postives = 385/483 (79.71%), Query Frame = 0


Query: 2   NRPALPHPK---IGKCKLRGLRFVCSAAA----DLFDDNLAAKNGNGAAAAAAAAALGLE 61
           NRP+   P    + K K R      SAAA    ++FD+    KNG     + AA+ LGL 
Sbjct: 24  NRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFDETPTTKNGK---PSVAASTLGLG 83

Query: 62  AETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASF 121
           AET+ + +DFFD+ +DPD PSDGYSSIQ+AVDAI QGKFVIVVDDEN D+EGNLVMAASF
Sbjct: 84  AETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIHQGKFVIVVDDENGDSEGNLVMAASF 143

Query: 122 TTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGN-ETSTFTITVDLKNGTSAGV 181
            TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S+DNGN ETSTFTITVD+K GTS GV
Sbjct: 144 ATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFSVDNGNNETSTFTITVDVKTGTSGGV 203

Query: 182 STADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPV 241
           S ADRAKTIVALSS ESK EDFRRPGHVFP+RYRDGGVLRRAGHTEASVDLV+LAGLRPV
Sbjct: 204 SAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRDGGVLRRAGHTEASVDLVALAGLRPV 263

Query: 242 SVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKW 301
           SVLS IVD +DGSMASLPK+R LAS+  IP+VSITDLIRYRRKRENLIERIAISRLPTKW
Sbjct: 264 SVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSITDLIRYRRKRENLIERIAISRLPTKW 323

Query: 302 GQFYAHCYRSNLDGIEHVALVK-------------------------------------- 361
           GQFYAHCYRS +DGIEHVALVK                                      
Sbjct: 324 GQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAM 383

Query: 362 -----AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGA 421
                AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN+ELGLPADARDYGVGA
Sbjct: 384 QMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANLELGLPADARDYGVGA 443

Query: 422 QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 433
           QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVT RVPVVT ITDENRKYLETKRTKMGHIY
Sbjct: 444 QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRVPVVTQITDENRKYLETKRTKMGHIY 503

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL
Match: A0A6J1G8U4 (GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1)

HSP 1 Score: 840 bits (2170), Expect = 1.17e-305
Identity = 443/490 (90.41%), Postives = 445/490 (90.82%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA-LGLEAETTP 60
           FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAA LGLEAETTP
Sbjct: 22  FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAAALGLEAETTP 81

Query: 61  DVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN 120
           DVVDFFDDEDDPDCPS+GYSSIQ AVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN
Sbjct: 82  DVVDFFDDEDDPDCPSEGYSSIQNAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSN 141

Query: 121 ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA 180
           ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA
Sbjct: 142 ITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRA 201

Query: 181 KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI 240
           KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI
Sbjct: 202 KTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTI 261

Query: 241 VDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH 300
           VDPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH
Sbjct: 262 VDPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAH 321

Query: 301 CYRSNLDGIEHVALVK-------------------------------------------A 360
           CYRSNLDGIEHVALVK                                           A
Sbjct: 322 CYRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKA 381

Query: 361 GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI 420
           GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI
Sbjct: 382 GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI 441

Query: 421 GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL 446
           GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL
Sbjct: 442 GIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFL 501

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL
Match: A0A6J1KB55 (GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1)

HSP 1 Score: 823 bits (2127), Expect = 3.58e-299
Identity = 434/489 (88.75%), Postives = 439/489 (89.78%), Query Frame = 0


Query: 1   FNRPALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALGLEAETTPD 60
           F+RPALPHPKIGKCK RGLRFVCSAAADLFDDNLAAKNGNGAA+AAA   LGLEAETTPD
Sbjct: 22  FHRPALPHPKIGKCKQRGLRFVCSAAADLFDDNLAAKNGNGAASAAA---LGLEAETTPD 81

Query: 61  VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 120
           VVDFFDDEDDPDCPS+GYSSI TAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI
Sbjct: 82  VVDFFDDEDDPDCPSEGYSSIPTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 141

Query: 121 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 180
           TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK
Sbjct: 142 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETSTFTITVDLKNGTSAGVSTADRAK 201

Query: 181 TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV 240
           TIVALSSP+SKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLR VSVLSTIV
Sbjct: 202 TIVALSSPQSKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRSVSVLSTIV 261

Query: 241 DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 300
           DPEDGSMASLPK+RKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC
Sbjct: 262 DPEDGSMASLPKLRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 321

Query: 301 YRSNLDGIEHVALVK-------------------------------------------AG 360
           YRSNLDGIEHVALVK                                           AG
Sbjct: 322 YRSNLDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAG 381

Query: 361 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 420
           RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG
Sbjct: 382 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 441

Query: 421 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 446
           IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR
Sbjct: 442 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQFLR 501

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL
Match: A0A6J1DY85 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1)

HSP 1 Score: 659 bits (1701), Expect = 4.09e-234
Identity = 356/483 (73.71%), Postives = 385/483 (79.71%), Query Frame = 0


Query: 2   NRPALPHPK---IGKCKLRGLRFVCSAAA----DLFDDNLAAKNGNGAAAAAAAAALGLE 61
           NRP+   P    + K K R      SAAA    ++FD+    KNG     + AA+ LGL 
Sbjct: 24  NRPSFAPPPPQTLRKSKQRTSNSASSAAAFSAGNMFDETPTTKNGK---PSVAASTLGLG 83

Query: 62  AETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASF 121
           AET+ + +DFFD+ +DPD PSDGYSSIQ+AVDAI QGKFVIVVDDEN D+EGNLVMAASF
Sbjct: 84  AETSSEAIDFFDNLEDPDHPSDGYSSIQSAVDAIHQGKFVIVVDDENGDSEGNLVMAASF 143

Query: 122 TTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGN-ETSTFTITVDLKNGTSAGV 181
            TP N+T+MVQHGSGI+YVGMKEDDL+RLKLP+ S+DNGN ETSTFTITVD+K GTS GV
Sbjct: 144 ATPGNVTSMVQHGSGIVYVGMKEDDLQRLKLPLFSVDNGNNETSTFTITVDVKTGTSGGV 203

Query: 182 STADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPV 241
           S ADRAKTIVALSS ESK EDFRRPGHVFP+RYRDGGVLRRAGHTEASVDLV+LAGLRPV
Sbjct: 204 SAADRAKTIVALSSSESKPEDFRRPGHVFPVRYRDGGVLRRAGHTEASVDLVALAGLRPV 263

Query: 242 SVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKW 301
           SVLS IVD +DGSMASLPK+R LAS+  IP+VSITDLIRYRRKRENLIERIAISRLPTKW
Sbjct: 264 SVLSAIVDTQDGSMASLPKLRSLASQHQIPMVSITDLIRYRRKRENLIERIAISRLPTKW 323

Query: 302 GQFYAHCYRSNLDGIEHVALVK-------------------------------------- 361
           GQFYAHCYRS +DGIEHVALVK                                      
Sbjct: 324 GQFYAHCYRSKVDGIEHVALVKGNIGDGQDVLVRVHSECLTGDIFASARCDCGNQLDLAM 383

Query: 362 -----AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGA 421
                AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEAN+ELGLPADARDYGVGA
Sbjct: 384 QMIEKAGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANLELGLPADARDYGVGA 443

Query: 422 QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 433
           QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVT RVPVVT ITDENRKYLETKRTKMGHIY
Sbjct: 444 QMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTGRVPVVTQITDENRKYLETKRTKMGHIY 503

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL
Match: A0A6J1DXS1 (GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1)

HSP 1 Score: 619 bits (1597), Expect = 3.75e-218
Identity = 340/478 (71.13%), Postives = 371/478 (77.62%), Query Frame = 0


Query: 10  KIGKCKLRGLRFVC-SAAADLFDDNL------AAKNGNGAAAAAAAAALGLEAETTPDVV 69
           KIGKCK +GLR+VC  +AA L   NL      A KN  G  +   +  LG+  E + +  
Sbjct: 41  KIGKCKQKGLRYVCLCSAAGLSAGNLFGESSHARKNNIGHESVGVSTNLGVNPEISFEGD 100

Query: 70  DFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITT 129
           DF DDEDDPD PSDG+SS+QTAVDAI  GKFV+VVDDEN D+EGNLVMAAS  + +NITT
Sbjct: 101 DFVDDEDDPDRPSDGFSSVQTAVDAIRHGKFVMVVDDENGDSEGNLVMAASKISATNITT 160

Query: 130 MVQHGSGILYVGMKEDDLERLKLPIISI-DNGN-ETSTFTITVDLKNGTSAGVSTADRAK 189
           MVQ  SG++YV MK DDLERL LP+IS+ DNGN ETSTFTITVD K  TS GVS ADRAK
Sbjct: 161 MVQQSSGMVYVAMKGDDLERLNLPLISLLDNGNYETSTFTITVDAKMETSTGVSAADRAK 220

Query: 190 TIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIV 249
           TIVALSSPESK EDFRRPGHVFP+RYRDGGVLRRAGHTEASVDLV+LAGLRPVSVLS IV
Sbjct: 221 TIVALSSPESKPEDFRRPGHVFPVRYRDGGVLRRAGHTEASVDLVALAGLRPVSVLSPIV 280

Query: 250 DPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHC 309
           D  DGSMASL K+R LAS+ +IPIVSITDLIRYRRKRENLIERI++SRLPTKWG+F A+C
Sbjct: 281 DAHDGSMASLSKLRSLASQHDIPIVSITDLIRYRRKRENLIERISVSRLPTKWGKFQAYC 340

Query: 310 YRSNLDGIEHVALVK-------------------------------------------AG 369
           YRS LDGIEHVA+VK                                           AG
Sbjct: 341 YRSKLDGIEHVAIVKGNIAEGQDVLVRVHSECLTGDIFASARCDCGNQLDLAMQMIEKAG 400

Query: 370 RGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 429
           RG VIYLRGHEGRGIGLG KLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG
Sbjct: 401 RGVVIYLRGHEGRGIGLGAKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIG 460

Query: 430 IRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIYAKGQF 435
           +RTMRLMTNNPAK KGLKGYGLA+T RVPVVTPITDENRKYLETKRTKMGH+YAK  F
Sbjct: 461 VRTMRLMTNNPAKLKGLKGYGLAMTGRVPVVTPITDENRKYLETKRTKMGHVYAKSPF 518

[Close]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL
Match: A0A5D2LNM4 (GTP cyclohydrolase II OS=Gossypium tomentosum OX=34277 GN=ES332_D03G161800v1 PE=3 SV=1)

HSP 1 Score: 572 bits (1473), Expect = 7.43e-200
Identity = 294/419 (70.17%), Postives = 346/419 (82.58%), Query Frame = 0


Query: 26  AADLFDDNLAAKNGNGAAAAAAAAALG-----LEAETTPDVVDFF--DDEDDPDCPSDGY 85
           A +LFDD     + NG+   A   ++      ++AE TP+ VDFF  D E DPDCPS G+
Sbjct: 49  AGNLFDDGSLKGSENGSLLGALGDSVSPPFGTVDAEITPETVDFFVSDAEGDPDCPSKGF 108

Query: 86  SSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKED 145
           SSI  A++ I QGKFVIVVDDENED EG L+MAAS  TP  +  MV+HGSGI+ VGMKE+
Sbjct: 109 SSIDQALNTIRQGKFVIVVDDENEDFEGTLIMAASHATPEMMAFMVKHGSGIVSVGMKEE 168

Query: 146 DLERLKLPIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDF 205
           DLERL+LP++S ++ ++ S   TFTITVD K GTS GVS  DRAKT++ALSSPESK +DF
Sbjct: 169 DLERLQLPLMSPESEDKDSSAPTFTITVDAKTGTSTGVSALDRAKTVLALSSPESKPDDF 228

Query: 206 RRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRK 265
           RRPGH+FPL+YR+GGVLRRAGHTEASVDLV L GLRPVSVLST+VDPEDGS+ASLP +RK
Sbjct: 229 RRPGHIFPLKYRNGGVLRRAGHTEASVDLVILTGLRPVSVLSTVVDPEDGSIASLPFIRK 288

Query: 266 LASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVK 325
           LA E +IP++SITDLIRYRRKRENL+ER AISRLPTKWG F A+CYRS LDG EH+A+VK
Sbjct: 289 LALEHSIPVISITDLIRYRRKRENLVERTAISRLPTKWGLFQAYCYRSKLDGTEHIAIVK 348

Query: 326 ----AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQ 385
               AGRG V+YLRGHEGRGIGLG KL AYNLQDQGHDTV+ANIELGL  DAR+YG+GAQ
Sbjct: 349 LIEQAGRGVVVYLRGHEGRGIGLGHKLRAYNLQDQGHDTVQANIELGLAVDAREYGIGAQ 408

Query: 386 MLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPITDENRKYLETKRTKMGHIY 430
           +LRD+G++TMRLMTNNPAKF GLKGYGLAV  RVPV+TP+T+EN++YLETKRTKMGHIY
Sbjct: 409 ILRDVGVQTMRLMTNNPAKFTGLKGYGLAVIGRVPVLTPVTEENKRYLETKRTKMGHIY 467

[Close]

BLAST of Cp4.1LG02g13280 vs. TAIR 10
Match: AT5G59750.1 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 528.9 bits (1361), Expect = 4.0e-150
Identity = 277/451 (61.42%), Postives = 337/451 (74.72%), Query Frame = 0


Query: 28  DLFDDNLAAKNGNGAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSDGYSSIQTAV 87
           ++FD+N   K       +A+     ++AE TP+  DFF  D E DPDCP+ GYSSI+ A+
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET--VDAEITPETDDFFVSDAEGDPDCPTQGYSSIELAL 110

Query: 88  DAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKL 147
            A+ +GKFVIVVDDE  D EGNL+MAA+ T+P +I  ++++GSGI+ VGMK+++LERL L
Sbjct: 111 QALRKGKFVIVVDDETGDVEGNLIMAATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSL 170

Query: 148 PIISIDNGNETS---TFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVF 207
            ++S +  +E S   TFTITVD K+GTS GVS +DRA T++ALSS ++K +DFRRPGHVF
Sbjct: 171 TLMSPEMEDEDSSAPTFTITVDAKSGTSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVF 230

Query: 208 PLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNI 267
           PL+YRDGGVLRRAGHTEASVDL+ LAGLRP+SVLS I+D EDGSMASLP M+KLA+E +I
Sbjct: 231 PLKYRDGGVLRRAGHTEASVDLMILAGLRPLSVLSAILDQEDGSMASLPYMKKLATEHDI 290

Query: 268 PIVSITDLIRYRRKRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVKA------ 327
           PIVS+TDLIRYRRKR+ L+ERI +SRLPTKWG F A+CYRS LDG E++ALVK       
Sbjct: 291 PIVSLTDLIRYRRKRDKLVERITVSRLPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGE 350

Query: 328 -------------------------------------GRGAVIYLRGHEGRGIGLGPKLL 387
                                                GRG V+YLRGHEGRGIGLG KL 
Sbjct: 351 DILVRVHSECLTGDIFGSARCDCGNQLDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLR 410

Query: 388 AYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGL 431
           AYNLQD+GHDTV+AN+ELGL  D+R+YG+GAQMLRDIG+RTMRLMTNNPAKF GLKGYGL
Sbjct: 411 AYNLQDEGHDTVQANVELGLSIDSREYGIGAQMLRDIGVRTMRLMTNNPAKFTGLKGYGL 470

[Close]

BLAST of Cp4.1LG02g13280 vs. TAIR 10
Match: AT5G59750.2 (DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II )

HSP 1 Score: 511.5 bits (1316), Expect = 6.7e-145
Identity = 277/485 (57.11%), Postives = 337/485 (69.48%), Query Frame = 0


Query: 28  DLFDDNLAAKNGNGAAAAAAAAALGLEAETTPDVVDFF--DDEDDPDCPSDGYSSIQTAV 87
           ++FD+N   K       +A+     ++AE TP+  DFF  D E DPDCP+ GYSSI+ A+
Sbjct: 51  NVFDENPVRKTDGSLFDSASFET--VDAEITPETDDFFVSDAEGDPDCPTQGYSSIELAL 110

Query: 88  DAISQGK----------------------------------FVIVVDDENEDTEGNLVMA 147
            A+ +GK                                  FVIVVDDE  D EGNL+MA
Sbjct: 111 QALRKGKVCSLTIFLKTSDYDDLFLQLYRFGLTKKLSLMFQFVIVVDDETGDVEGNLIMA 170

Query: 148 ASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNETS---TFTITVDLKNG 207
           A+ T+P +I  ++++GSGI+ VGMK+++LERL L ++S +  +E S   TFTITVD K+G
Sbjct: 171 ATLTSPKDIAFLIKNGSGIVSVGMKKENLERLSLTLMSPEMEDEDSSAPTFTITVDAKSG 230

Query: 208 TSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLA 267
           TS GVS +DRA T++ALSS ++K +DFRRPGHVFPL+YRDGGVLRRAGHTEASVDL+ LA
Sbjct: 231 TSTGVSASDRAMTVLALSSLDAKPDDFRRPGHVFPLKYRDGGVLRRAGHTEASVDLMILA 290

Query: 268 GLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISR 327
           GLRP+SVLS I+D EDGSMASLP M+KLA+E +IPIVS+TDLIRYRRKR+ L+ERI +SR
Sbjct: 291 GLRPLSVLSAILDQEDGSMASLPYMKKLATEHDIPIVSLTDLIRYRRKRDKLVERITVSR 350

Query: 328 LPTKWGQFYAHCYRSNLDGIEHVALVKA-------------------------------- 387
           LPTKWG F A+CYRS LDG E++ALVK                                 
Sbjct: 351 LPTKWGLFQAYCYRSKLDGTENIALVKGNVGNGEDILVRVHSECLTGDIFGSARCDCGNQ 410

Query: 388 -----------GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARD 431
                      GRG V+YLRGHEGRGIGLG KL AYNLQD+GHDTV+AN+ELGL  D+R+
Sbjct: 411 LDLAMELIEKEGRGVVVYLRGHEGRGIGLGHKLRAYNLQDEGHDTVQANVELGLSIDSRE 470

[Close]

BLAST of Cp4.1LG02g13280 vs. TAIR 10
Match: AT5G64300.1 (GTP cyclohydrolase II )

HSP 1 Score: 464.5 bits (1194), Expect = 9.3e-131
Identity = 248/456 (54.39%), Postives = 316/456 (69.30%), Query Frame = 0


Query: 39  GNGAAAAAAAAALGLEAETTPDVVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVD 98
           G  AA +A A A G  AE         DD+ + D P+ G+SSI  A++ I QGK V+VVD
Sbjct: 98  GTLAADSAPAPANGFVAE---------DDDFELDLPTPGFSSIPEAIEDIRQGKLVVVVD 157

Query: 99  DENEDTEGNLVMAASFTTPSNITTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE--- 158
           DE+ + EG+LVMAA   TP  +  +V+HG+GI+ V MKEDDLERL LP++     NE   
Sbjct: 158 DEDRENEGDLVMAAQLATPEAMAFIVRHGTGIVCVSMKEDDLERLHLPLMVNQKENEEKL 217

Query: 159 TSTFTITVDLKNGTSAGVSTADRAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRA 218
           ++ FT+TVD K+GT+ GVS  DRA TI++L+S +SK EDF RPGH+FPL+YR+GGVL+RA
Sbjct: 218 STAFTVTVDAKHGTTTGVSARDRATTILSLASRDSKPEDFNRPGHIFPLKYREGGVLKRA 277

Query: 219 GHTEASVDLVSLAGLRPVSVLSTIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRR 278
           GHTEASVDL  LAGL PV VL  IVD +DGSMA LPK+R+ A+E N+ +VSI DLIRYRR
Sbjct: 278 GHTEASVDLTVLAGLDPVGVLCEIVD-DDGSMARLPKLREFAAENNLKVVSIADLIRYRR 337

Query: 279 KRENLIERIAISRLPTKWGQFYAHCYRSNLDGIEHVALVK-------------------- 338
           KR+ L+ER + +R+PT WG F A+CYRS LDGIEH+A+VK                    
Sbjct: 338 KRDKLVERASAARIPTMWGPFTAYCYRSILDGIEHIAMVKGEIGDGQDILVRVHSECLTG 397

Query: 339 -----------------------AGRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVE 398
                                   GRG ++YLRGHEGRGIGLG KL AYNLQD G DTVE
Sbjct: 398 DIFGSARCDCGNQLALSMQQIEATGRGVLVYLRGHEGRGIGLGHKLRAYNLQDAGRDTVE 457

Query: 399 ANIELGLPADARDYGVGAQMLRDIGIRTMRLMTNNPAKFKGLKGYGLAVTARVPVVTPIT 446
           AN ELGLP D+R+YG+GAQ++RD+G+RTM+LMTNNPAK+ GLKGYGLA+  RVP+++ IT
Sbjct: 458 ANEELGLPVDSREYGIGAQIIRDLGVRTMKLMTNNPAKYVGLKGYGLAIVGRVPLLSLIT 517

[Close]

BLAST of Cp4.1LG02g13280 vs. TAIR 10
Match: AT2G22450.1 (riboflavin biosynthesis protein, putative )

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-102
Identity = 208/453 (45.92%), Postives = 281/453 (62.03%), Query Frame = 0


Query: 5   ALPHPKIGKCKLRGLRFVCSAAADLFDDNLAAKNGNGAAAAAAAAALG-LEAE---TTPD 64
           +L +P+I   K      V     D+F  + +  +  G        + G L AE   TT D
Sbjct: 29  SLVYPRIISKKFNVRMRVIPEEGDVFSSSKSNGSSMGIELQPDLVSFGTLAAEMIPTTMD 88

Query: 65  VVDFFDDEDDPDCPSDGYSSIQTAVDAISQGKFVIVVDDENEDTEGNLVMAASFTTPSNI 124
             +  D+E D D P+DG++SI  A++ I  GK V+VVDDE+ + EG+L+MAAS  TP  +
Sbjct: 89  SPEVEDEEFDLDRPTDGFASIPQAIEDIRHGKMVVVVDDEDRENEGDLIMAASLATPEAM 148

Query: 125 TTMVQHGSGILYVGMKEDDLERLKLPIISIDNGNE---TSTFTITVDLKNGTSAGVSTAD 184
             +V+HG+GI+ V MK +DLERL+LP++     NE    + FT++VD K GTS GVS  D
Sbjct: 149 AFVVKHGTGIVCVSMKGEDLERLELPLMVTRKDNEEKLRTAFTVSVDAKKGTSTGVSARD 208

Query: 185 RAKTIVALSSPESKAEDFRRPGHVFPLRYRDGGVLRRAGHTEASVDLVSLAGLRPVSVLS 244
           RA+TI+ L+S +SK EDF RPGH+FPLRYR+GGVL+RAGHTEASVDL  LAGL PVSVL 
Sbjct: 209 RAQTILTLASKDSKPEDFNRPGHIFPLRYREGGVLKRAGHTEASVDLTVLAGLEPVSVLC 268

Query: 245 TIVDPEDGSMASLPKMRKLASEKNIPIVSITDLIRYRRKRENLIERIAISRLPTKWGQFY 304
            IVD +DGSMA LP++R+ A E N+ ++SI DLIRYRRKRE L+E  A++ +PT WG F 
Sbjct: 269 EIVD-DDGSMARLPRLRQFAQENNLKLISIADLIRYRRKRERLVEFTAVAPIPTMWGPFK 328

Query: 305 AHCYRSNLDGIEHVALVKA----------------------------------------- 364
           AHC++S LDG+EH+A+VK                                          
Sbjct: 329 AHCFKSLLDGVEHIAMVKGEIGDGKDILVRVHAECITDDIFGNSSGGKQLAIAMRLIEEN 388

Query: 365 GRGAVIYLRGHEGRGIGLGPKLLAYNLQDQGHDTVEANIELGLPADARDYGVGAQMLRDI 410
           GRG  +YLRG E +GI L  K   YN      D  E    +  P  +R+YG+GAQ+LRD+
Sbjct: 389 GRGVFVYLRGPESKGIDLSHKPRTYNTNS---DQAEG---VSFPVASREYGIGAQILRDL 448

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g13280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FN89	5.7e-149	61.42	Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS=Arabidop...	[more]
Q6L506	1.3e-137	55.72	Probable monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic OS...	[more]
Q6Z234	2.6e-133	56.45	Probable bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=O...	[more]
Q0E079	1.3e-132	57.38	Probable bifunctional riboflavin biosynthesis protein RIBA 2, chloroplastic OS=O...	[more]
P47924	1.3e-129	54.39	Bifunctional riboflavin biosynthesis protein RIBA 1, chloroplastic OS=Arabidopsi...	[more]

BLAST of Cp4.1LG02g13280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023522983.1	5.16e-309	91.21	monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur...	[more]
XP_022948312.1	2.42e-305	90.41	monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur...	[more]
KAG6607228.1	8.02e-302	89.41	Monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic, partial [C...	[more]
XP_022998776.1	7.40e-299	88.75	monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Cucur...	[more]
XP_022159223.1	8.45e-234	73.71	monofunctional riboflavin biosynthesis protein RIBA 3, chloroplastic-like [Momor...	[more]

BLAST of Cp4.1LG02g13280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G8U4	1.17e-305	90.41	GTP cyclohydrolase II OS=Cucurbita moschata OX=3662 GN=LOC111452026 PE=3 SV=1	[more]
A0A6J1KB55	3.58e-299	88.75	GTP cyclohydrolase II OS=Cucurbita maxima OX=3661 GN=LOC111493343 PE=3 SV=1	[more]
A0A6J1DY85	4.09e-234	73.71	GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111025642 PE=3 SV=1	[more]
A0A6J1DXS1	3.75e-218	71.13	GTP cyclohydrolase II OS=Momordica charantia OX=3673 GN=LOC111024475 PE=3 SV=1	[more]
A0A5D2LNM4	7.43e-200	70.17	GTP cyclohydrolase II OS=Gossypium tomentosum OX=34277 GN=ES332_D03G161800v1 PE=...	[more]

BLAST of Cp4.1LG02g13280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59750.1	4.0e-150	61.42	DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II	[more]
AT5G59750.2	6.7e-145	57.11	DHBP synthase RibB-like alpha/beta domain;GTP cyclohydrolase II	[more]
AT5G64300.1	9.3e-131	54.39	GTP cyclohydrolase II	[more]
AT2G22450.1	1.4e-102	45.92	riboflavin biosynthesis protein, putative	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000422	3,4-dihydroxy-2-butanone 4-phosphate synthase, RibB	PFAM	PF00926	DHBP_synthase	coord: 83..273 e-value: 2.7E-68 score: 229.3
IPR000422	3,4-dihydroxy-2-butanone 4-phosphate synthase, RibB	TIGRFAM	TIGR00506	TIGR00506	coord: 80..274 e-value: 4.5E-57 score: 190.8
None	No IPR available	GENE3D	3.90.870.10	DHBP synthase	coord: 71..281 e-value: 1.3E-75 score: 255.2
None	No IPR available	PIRSF	PIRSF001259	RibA	coord: 76..317 e-value: 2.7E-94 score: 314.2 coord: 315..440 e-value: 2.5E-54 score: 182.6
None	No IPR available	PANTHER	PTHR21327:SF29	MONOFUNCTIONAL RIBOFLAVIN BIOSYNTHESIS PROTEIN RIBA 3, CHLOROPLASTIC	coord: 315..431 coord: 44..315
None	No IPR available	PANTHER	PTHR21327	GTP CYCLOHYDROLASE II-RELATED	coord: 315..431 coord: 44..315
IPR036144	GTP cyclohydrolase II superfamily	GENE3D	3.40.50.10990	GTP cyclohydrolase II	coord: 282..315 e-value: 1.2E-5 score: 27.1
IPR036144	GTP cyclohydrolase II superfamily	GENE3D	3.40.50.10990	GTP cyclohydrolase II	coord: 316..409 e-value: 3.0E-39 score: 136.1
IPR036144	GTP cyclohydrolase II superfamily	SUPERFAMILY	142695	RibA-like	coord: 280..408
IPR032677	GTP cyclohydrolase II	PFAM	PF00925	GTP_cyclohydro2	coord: 315..406 e-value: 4.6E-38 score: 130.4
IPR000926	GTP cyclohydrolase II, RibA	CDD	cd00641	GTP_cyclohydro2	coord: 280..429 e-value: 4.75665E-76 score: 233.931
IPR017945	DHBP synthase RibB-like alpha/beta domain superfamily	SUPERFAMILY	55821	YrdC/RibB	coord: 78..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g13280.1	Cp4.1LG02g13280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009231	riboflavin biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0008686	3,4-dihydroxy-2-butanone-4-phosphate synthase activity
molecular_function	GO:0005525	GTP binding
molecular_function	GO:0003935	GTP cyclohydrolase II activity
molecular_function	GO:0046872	metal ion binding