Cp4.1LG02g11730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g11730
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like
Location	Cp4.1LG02: 10700601 .. 10701845 (-)
RNA-Seq Expression	Cp4.1LG02g11730
Synteny	Cp4.1LG02g11730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACCTGTCGCCGTCATTTTCGGAGTTACCGGTTTGGTCGGAAAAGAGTTAGCAAAAACCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATTCTAATTTCCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATCTTTTCTGGGTAACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTTGACGTCATTCTCCCGGCGGCGTCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGGCGGAGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACAGTACGCCACTCTTTCACTATTAATTTATTTTTAATAATTAATTTAAATTTTCCATAAAAATATTTTATGTGTAAATTCCAACGACAGCCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGGCGGAGAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAATTCCGACGGACATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGGTTCAGTGTCAAGTACAAGATGTTGGACTCCATCCTGTATTGGATCGATGTCATGCGACACGACAAATTAATTCCTTTTCCTTAA

mRNA sequence

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACCTGTCGCCGTCATTTTCGGAGTTACCGGTTTGGTCGGAAAAGAGTTAGCAAAAACCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATTCTAATTTCCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATCTTTTCTGGGTAACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTTGACGTCATTCTCCCGGCGGCGTCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGGCGGAGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGGCGGAGAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAATTCCGACGGACATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGGTTCAGTGTCAAGTACAAGATGTTGGACTCCATCCTGTATTGGATCGATGTCATGCGACACGACAAATTAATTCCTTTTCCTTAA

Coding sequence (CDS)

ATGGCGGCCGAAACAATCAATGCAAAATTAGACGGCAATGGCGAACCTGTCGCCGTCATTTTCGGAGTTACCGGTTTGGTCGGAAAAGAGTTAGCAAAAACCCTTCTCTCAACCGGCGGCTGGAAAGTTTACGGCGTCGCACGGCGGCCGGATAATGTCAGCCCCATCACCGATTCTAATTTCCAATTCATTTCCTGCGATTTGCTCGACCGCTCTTCCGCTCACCGAAACCTCTCGCCGGTTCGCCACGTCACACATCTTTTCTGGGTAACTTGGGCTGCCCAGCTACGGTTCGATACCTCAGATTGCTCGGACCAGAACCGGACCATGTTGGCTAACGTCCTTGACGTCATTCTCCCGGCGGCGTCGGCTCTCCGCCACGTCTCTCTACAGACCGGGATTAAGCACTACGTTTCGTTGACGGGAGGCGGAGGCGGTCGGATGGCGGTTTATTGTGAGGATAGTCCGAGGGCGGAATCTGGGAACAATTTTTATTATGCGTTGGAGGATCTCCTCCGTGAGCGGCTGTCCGGCGGCGGGCGGAGGGTGGCGTGGAGTGTTCTTCGACCGGGTTTGATTTTGGGCTGTTCGAACCGGACTTTTTTTAATTTTATGGGGAGTTTGTGTGTTTATGGAGCTATTTGTAAGAAGCTGAAGAAGCCGTTTGTGTTCGGCGGCTCGGCGGCGTGCTGGGAAGGGTTTTGTATCAATGGCTCCGACGCTCGGCTTGTGGCGGAGCAGCACGTTTGGGCCGCCACCAATGAAATAAACACCACCGCAGACGGGGAAGCATTCAACGTGTGCAACGGGTGGAGCTTCACGTGGAAGGAGATATGGGGAGCGGTGGCGGAGAAGTTTGGAGTGGCGGCGGAGGACGAGGAGGGGATGTTTTCTCCGGAGTTCCATTACACGGCAGCGATGGCGGAGAAAGGGAAGGTGTGGGCGGAGATAGTGGAGGAGGAAGGGCTAATTCCGACGGACATGGAGGAGCTGGCCAATTGGGGGTTCTTAGACAGCTTGTTTAGGCTGCCGGGAAAAATGGTGGCTAGTCGGAACAAATCCGACCGGCTTGGGTTCAGTGTCAAGTACAAGATGTTGGACTCCATCCTGTATTGGATCGATGTCATGCGACACGACAAATTAATTCCTTTTCCTTAA

Protein sequence

MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSLQTGIKHYVSLTGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDSILYWIDVMRHDKLIPFP

Homology

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.6e-67
Identity = 157/377 (41.64%), Postives = 209/377 (55.44%), Query Frame = 0


Query: 15  EPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLD 74
           + VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D   ++I CD+ D
Sbjct: 26  QSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYGVARRP-RPAWNADHPVEYIQCDISD 85

Query: 75  RSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSL 134
            S  H  LSP+  VTH+FWVTWA   R   S+C + N TML NVL+ ++P A+ L H+ L
Sbjct: 86  ASDTHTKLSPLTDVTHIFWVTWAN--RPTESECCELNGTMLRNVLNALIPKAANLHHICL 145

Query: 135 QTGIKHYVSLTGGGG---GRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSV 194
           QTG KHY+      G        + ED PR  +  NFYY LED+L E  S     + WSV
Sbjct: 146 QTGHKHYIGPFEAFGKIKPHEPPFTEDMPRL-NAPNFYYTLEDMLVE-ASEKKAGLNWSV 205

Query: 195 LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQ 254
            RP +I G S  +  N +G+LCVY AICK    P  F G+ A W  + +  SDA L+AE 
Sbjct: 206 HRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLKFPGTKAAWNCYSV-ASDADLIAEH 265

Query: 255 HVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPEFHYTAAM 314
            +WAA   ++  A  EAFN  NG  F WK +W  +AE+FGV   + +    P       M
Sbjct: 266 QIWAA---VDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFGVEYAEFDESEKPT-SLVERM 325

Query: 315 AEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGK-MVASRNKSDRLGFSVKYKML 374
            +KG VW EIV E GL  T +E +A W F D +  L G+ ++ S NKS   G+       
Sbjct: 326 KDKGPVWEEIVRENGLHTTKLEGVATWWFADVI--LGGECLLDSMNKSKEHGYLGFRNTK 385

Query: 375 DSILYWIDVMRHDKLIP 384
           +S++  ID M+  K++P
Sbjct: 386 NSLISVIDKMKAHKIVP 390

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.6e-65
Identity = 156/376 (41.49%), Postives = 202/376 (53.72%), Query Frame = 0


Query: 15  EPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSL 134
                  LSP+  VTH+F+VTW    R   S+  + N +ML NVL  I+P A  LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVS-LTGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 194
           QTG KHY+   T   G R    + ED PR +   NFYY  ED+L E +      V WS+ 
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTWSIH 205

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+AEQ 
Sbjct: 206 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIAEQQ 265

Query: 255 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAED-EEGMFSPEFHYTAAM 314
           +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     EEG           M
Sbjct: 266 IWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEG---KNLGLVEMM 325

Query: 315 AEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLD 374
             K +VW E+V+E  L    +EE+  W F D +  + G M+ S NKS   GF       +
Sbjct: 326 KGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSNN 385

Query: 375 SILYWIDVMRHDKLIP 384
           S + WID  +  K++P
Sbjct: 386 SFISWIDKYKAFKIVP 388

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.6e-62
Identity = 153/378 (40.48%), Postives = 200/378 (52.91%), Query Frame = 0


Query: 13  NGEPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDL 72
           N E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 73  LDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHV 132
           LD       LSP+  VTH+F+ TW    R    +  + N  ML NVL+ ++P    L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGIKHYVSLTGGGGGRM--AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWS 192
            LQTG KHY+        +       ED PR +S  NFYY  ED+L E +      + WS
Sbjct: 144 CLQTGRKHYLGAFEDWKIKRHDPPLTEDLPRLDS-QNFYYTQEDILFEEVQ-KKESLTWS 203

Query: 193 VLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAE 252
           V RPG I G S  +  N +G+LCVY AICK       F G    W+G+  + SDA L+AE
Sbjct: 204 VHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDGYS-DCSDADLIAE 263

Query: 253 QHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAEDEEGMFSPEFHYTA 312
             +WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV   E EEG    E     
Sbjct: 264 HQIWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEEG---KEVKLQD 323

Query: 313 AMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKM 372
            M +KG VW +IV E GL  T +E++ NW F D +       + + NKS   GF      
Sbjct: 324 LMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGFRNS 383

Query: 373 LDSILYWIDVMRHDKLIP 384
            +S + WID ++  K++P
Sbjct: 384 KNSFISWIDKVKAFKIVP 388

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.0e-62
Identity = 150/375 (40.00%), Postives = 201/375 (53.60%), Query Frame = 0


Query: 17  VAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLDRS 76
           VA+I GVTG++G  LA+ L       G WKVYGVARR    +   D+   ++ CD+ D  
Sbjct: 28  VALIVGVTGIIGNSLAEILPLADTPGGPWKVYGVARR-TRPAWHEDNPINYVQCDISDPD 87

Query: 77  SAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSLQT 136
            +   LSP+  VTH+F+VTWA   R    +  + N  M  NVLD ++P    L+H+SLQT
Sbjct: 88  DSQAKLSPLTDVTHVFYVTWAN--RSTEQENCEANSKMFRNVLDAVIPNCPNLKHISLQT 147

Query: 137 GIKHYVSLTGGGG---GRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVLR 196
           G KHY+      G        Y ED PR +   NFYY LED++ E +      + WSV R
Sbjct: 148 GRKHYMGPFESYGKIESHDPPYTEDLPRLKY-MNFYYDLEDIMLEEVE-KKEGLTWSVHR 207

Query: 197 PGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQHV 256
           PG I G S  +  N +G+LCVY AICK   K   F G  A W+G+  + SDA L+AE H+
Sbjct: 208 PGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLRFTGCKAAWDGYS-DCSDADLIAEHHI 267

Query: 257 WAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAEDEEGMFSPEFHYTAAMA 316
           WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV   E EEG+   +      M 
Sbjct: 268 WAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVGCGEYEEGV---DLKLQDLMK 327

Query: 317 EKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLDS 376
            K  VW EIV E GL PT ++++  W F D +       + S NKS   GF       ++
Sbjct: 328 GKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILG-NECFLDSMNKSKEHGFLGFRNSKNA 387

Query: 377 ILYWIDVMRHDKLIP 384
            + WID  +  K++P
Sbjct: 388 FISWIDKAKAYKIVP 389

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.0e-62
Identity = 151/381 (39.63%), Postives = 199/381 (52.23%), Query Frame = 0


Query: 13  NGEPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDL 72
           N E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   S   D    +ISCD+
Sbjct: 24  NYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPSWNEDHPITYISCDV 83

Query: 73  LDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHV 132
           LD       LSP+  VTH+F+ TW    R    +  + N  ML NVL+ ++P    L+H+
Sbjct: 84  LDSVDVEAKLSPLTDVTHIFYATWTK--RSTEKENCEANGKMLKNVLNAMIPNCPNLKHI 143

Query: 133 SLQTGIKHYVSLTGGGGGRMAVYCEDSPRAE-----SGNNFYYALEDLLRERLSGGGRRV 192
            LQTG KHY+    G    +   C D P  E        N+YY  ED+L E +      +
Sbjct: 144 CLQTGRKHYL----GAFEDLKSKCHDPPLTEDLPRLDSQNYYYTQEDILFEEVQ-KKEGL 203

Query: 193 AWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARL 252
            WSV RPG I G S  +  N +G+LCVY AICK       F G    W+G+  + SDA L
Sbjct: 204 TWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLRFPGCKGAWDGYS-DCSDADL 263

Query: 253 VAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGV-AAEDEEGMFSPEFH 312
           +AE  +WAA   ++  A  EAFNV NG  F WK  W  +AE+FGV   E EEG    +  
Sbjct: 264 IAEHQIWAA---VDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFGVECGEYEEGQ---QVK 323

Query: 313 YTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVK 372
               M +KG VW +IV E GL  T +E++ NW F D +       + + NKS   GF   
Sbjct: 324 LQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLG-NECWLDTMNKSKEHGFLGF 383

Query: 373 YKMLDSILYWIDVMRHDKLIP 384
               +S + WID ++  K++P
Sbjct: 384 RNSKNSFISWIDKVKAFKIVP 388

[Close]

BLAST of Cp4.1LG02g11730 vs. NCBI nr
Match: XP_023525980.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796 bits (2056), Expect = 4.19e-291
Identity = 385/385 (100.00%), Postives = 385/385 (100.00%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN
Sbjct: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGG 180
           AASALRHVSLQTGIKHYVSLTGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGG
Sbjct: 121 AASALRHVSLQTGIKHYVSLTGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGG 180

Query: 181 RRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSD 240
           RRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSD
Sbjct: 181 RRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSD 240

Query: 241 ARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPE 300
           ARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPE
Sbjct: 241 ARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPE 300

Query: 301 FHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFS 360
           FHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFS
Sbjct: 301 FHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFS 360

Query: 361 VKYKMLDSILYWIDVMRHDKLIPFP 385
           VKYKMLDSILYWIDVMRHDKLIPFP
Sbjct: 361 VKYKMLDSILYWIDVMRHDKLIPFP 385

[Close]

BLAST of Cp4.1LG02g11730 vs. NCBI nr
Match: XP_022948773.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata])

HSP 1 Score: 768 bits (1982), Expect = 1.00e-279
Identity = 375/391 (95.91%), Postives = 381/391 (97.44%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLTG    GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA--EDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA  EDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAM EKG++WAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of Cp4.1LG02g11730 vs. NCBI nr
Match: KAG6607304.1 ((S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 766 bits (1977), Expect = 5.81e-279
Identity = 375/391 (95.91%), Postives = 380/391 (97.19%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           M AETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MTAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLTG    GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA--EDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA  EDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAM EKG+VWAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of Cp4.1LG02g11730 vs. NCBI nr
Match: KAG7036983.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 765 bits (1975), Expect = 1.17e-278
Identity = 375/391 (95.91%), Postives = 380/391 (97.19%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSA+RNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSANRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLTG    GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA--EDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA  EDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHY AAM EKG+VWAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYMAAMTEKGRVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of Cp4.1LG02g11730 vs. NCBI nr
Match: XP_022998181.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 764 bits (1974), Expect = 1.79e-278
Identity = 375/393 (95.42%), Postives = 380/393 (96.69%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTW AQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG-----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
           AA ALRHVSLQTGIKHYVSLTG     GGGG MAVYCEDSPRAESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240
           LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA---ED 300
           INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFG+AA   ED
Sbjct: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAED 300

Query: 301 EEGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRN 360
           E+GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRN
Sbjct: 301 EKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           KSDRLGFSVKYKMLDSILYWIDVMR+D LIPFP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDTLIPFP 393

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL
Match: A0A6J1GA93 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111452342 PE=3 SV=1)

HSP 1 Score: 768 bits (1982), Expect = 4.86e-280
Identity = 375/391 (95.91%), Postives = 381/391 (97.44%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAK LLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKALLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTH+FWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHVFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180
           AA ALRHVSLQTGIKHYVSLTG    GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERL 180

Query: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240
           SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI
Sbjct: 181 SGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCI 240

Query: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA--EDEE 300
           NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA  EDEE
Sbjct: 241 NGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAAAEDEE 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
           GMFSPEFHYTAAM EKG++WAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRNKS
Sbjct: 301 GMFSPEFHYTAAMTEKGRLWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRNKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGFSVKYKMLDSILYWIDVMR+DKLIPFP
Sbjct: 361 DRLGFSVKYKMLDSILYWIDVMRYDKLIPFP 391

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL
Match: A0A6J1K9J8 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492905 PE=3 SV=1)

HSP 1 Score: 764 bits (1974), Expect = 8.69e-279
Identity = 375/393 (95.42%), Postives = 380/393 (96.69%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MAAETINAKLDGNGE VAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITD+N
Sbjct: 1   MAAETINAKLDGNGELVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDTN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTW AQLRFDTSDCSDQNRTMLANVLDVILP
Sbjct: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWTAQLRFDTSDCSDQNRTMLANVLDVILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG-----GGGGRMAVYCEDSPRAESGNNFYYALEDLLRER 180
           AA ALRHVSLQTGIKHYVSLTG     GGGG MAVYCEDSPRAESGNNFYYALEDLLRER
Sbjct: 121 AAPALRHVSLQTGIKHYVSLTGAAVDGGGGGGMAVYCEDSPRAESGNNFYYALEDLLRER 180

Query: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240
           LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC
Sbjct: 181 LSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFC 240

Query: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA---ED 300
           INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFG+AA   ED
Sbjct: 241 INGSDARLVAEQHVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGLAAAAAED 300

Query: 301 EEGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRN 360
           E+GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPT+MEELANWGFLDSLFRLPGKMVASRN
Sbjct: 301 EKGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTEMEELANWGFLDSLFRLPGKMVASRN 360

Query: 361 KSDRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           KSDRLGFSVKYKMLDSILYWIDVMR+D LIPFP
Sbjct: 361 KSDRLGFSVKYKMLDSILYWIDVMRYDTLIPFP 393

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL
Match: A0A6J1I0N6 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111469437 PE=3 SV=1)

HSP 1 Score: 645 bits (1665), Expect = 9.38e-232
Identity = 321/391 (82.10%), Postives = 337/391 (86.19%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MA E++N  LD NGE VAVIFGVTGLVGKELAK LLST GWKVYGVARRPDN+SPI+D N
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR SAH NLSPVRHVTH+FW+TWAAQLRFD+ DCSDQN+ MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPVRHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTGG--GGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSG 180
           +ASALRHVSLQTG+KHY SLT     GG   VY EDSPRAESGNNFYY LEDLLRERLSG
Sbjct: 121 SASALRHVSLQTGVKHYASLTSSTSSGGGEVVYSEDSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 GGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCING 240
             R VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I+G
Sbjct: 181 YRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDG 240

Query: 241 SDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVA--AEDEE 300
           SDARL AEQHVWAAT   EIN   D EAFNVCNGWSFTWKEIWGAVAEK GV   AEDE 
Sbjct: 241 SDARLTAEQHVWAATQAVEINAITDREAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
            MFSPEFHYT AMAEKG VWAEIVEEEGL+ T+M ELANWGFLDSLFRLP KMVASR KS
Sbjct: 301 -MFSPEFHYTEAMAEKGMVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGF+VKYKMLDSILYWIDVMRHDKLIP P
Sbjct: 361 DRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL
Match: A0A0A0LVI2 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 6.76e-231
Identity = 319/395 (80.76%), Postives = 337/395 (85.32%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MA E IN  LD NGE VAVIFGVTGLVGK+LAKTLLST GWKVYGVARRPDNVSPI+   
Sbjct: 1   MAVEIINTNLDANGELVAVIFGVTGLVGKQLAKTLLSTAGWKVYGVARRPDNVSPISHPK 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR S  +NLSPVRHVTHLFW+TWAAQLR D+ DCSDQNR MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSVQQNLSPVRHVTHLFWITWAAQLRLDSPDCSDQNRAMLANALDAILP 120

Query: 121 AASALRHVSLQTGIKHYVSLT------GGGGGRMAVYCEDSPRAESGNNFYYALEDLLRE 180
           +A ALRHVSLQTGIKHY SLT      GGGG    VYCEDSPRAESGNNFYY LEDLLRE
Sbjct: 121 SAPALRHVSLQTGIKHYASLTRLAAGCGGGGEGEVVYCEDSPRAESGNNFYYVLEDLLRE 180

Query: 181 RLSGGGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGF 240
           RLS G R VAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE  
Sbjct: 181 RLSCGRRMVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEV 240

Query: 241 CINGSDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAA-- 300
            I+GSDARL AEQH+W AT   EIN TADGEAFNVCNGWSF WKEIWGAVAEK GVA   
Sbjct: 241 YIDGSDARLTAEQHIWVATKAVEINATADGEAFNVCNGWSFRWKEIWGAVAEKLGVATTG 300

Query: 301 --EDEEGMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMV 360
             E++E MFS EFHYTAAM +KGKVWAEIVEEEGL+ T+MEELANWGFLD+LFRLP KMV
Sbjct: 301 AEEEKERMFSKEFHYTAAMGDKGKVWAEIVEEEGLVTTEMEELANWGFLDTLFRLPEKMV 360

Query: 361 ASRNKSDRLGFSVKYKMLDSILYWIDVMRHDKLIP 383
           ASR KSDRLGF+VKYKMLDS+LYWIDVMR+DKLIP
Sbjct: 361 ASRAKSDRLGFNVKYKMLDSMLYWIDVMRNDKLIP 395

[Close]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL
Match: A0A6J1HM54 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111464842 PE=3 SV=1)

HSP 1 Score: 640 bits (1651), Expect = 1.27e-229
Identity = 318/391 (81.33%), Postives = 336/391 (85.93%), Query Frame = 0


Query: 1   MAAETINAKLDGNGEPVAVIFGVTGLVGKELAKTLLSTGGWKVYGVARRPDNVSPITDSN 60
           MA E++N  LD NGE VAVIFGVTGLVGKELAK LLST GWKVYGVARRPDN+SPI+D N
Sbjct: 1   MADESMNTNLDANGELVAVIFGVTGLVGKELAKMLLSTAGWKVYGVARRPDNISPISDPN 60

Query: 61  FQFISCDLLDRSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILP 120
           F FISCDLLDR SAH NLSP+ HVTH+FW+TWAAQLRFD+ DCSDQN+ MLAN LD ILP
Sbjct: 61  FHFISCDLLDRRSAHLNLSPLGHVTHMFWITWAAQLRFDSQDCSDQNKAMLANALDAILP 120

Query: 121 AASALRHVSLQTGIKHYVSLTG--GGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSG 180
           +A ALRHVSLQTG+KHY SLT   G GG   VY E+SPRAESGNNFYY LEDLLRERLSG
Sbjct: 121 SAPALRHVSLQTGVKHYASLTSSTGSGGGEVVYSENSPRAESGNNFYYVLEDLLRERLSG 180

Query: 181 GGRRVAWSVLRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCING 240
             R VAWS+LRPGLILGCSNRTFFNFMGSLCVYGAICKKLK PFVFGG+AACWE   I+ 
Sbjct: 181 RRRVVAWSILRPGLILGCSNRTFFNFMGSLCVYGAICKKLKMPFVFGGTAACWEEVYIDS 240

Query: 241 SDARLVAEQHVWAATN--EINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVA--AEDEE 300
           SDARL AEQHVWAAT   EIN   DGEAFNVCNGWSFTWKEIWGAVAEK GV   AEDE 
Sbjct: 241 SDARLTAEQHVWAATKAVEINALTDGEAFNVCNGWSFTWKEIWGAVAEKLGVTVGAEDER 300

Query: 301 GMFSPEFHYTAAMAEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKS 360
            MFSPEF YTAAMAEKG VWAEIVEEEGL+ T+M ELANWGFLDSLFRLP KMVASR KS
Sbjct: 301 -MFSPEFRYTAAMAEKGTVWAEIVEEEGLVATEMGELANWGFLDSLFRLPEKMVASRAKS 360

Query: 361 DRLGFSVKYKMLDSILYWIDVMRHDKLIPFP 385
           DRLGF+VKYKMLDSILYWIDVMRHDKLIP P
Sbjct: 361 DRLGFTVKYKMLDSILYWIDVMRHDKLIPSP 390

[Close]

BLAST of Cp4.1LG02g11730 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.4e-113
Identity = 206/376 (54.79%), Postives = 261/376 (69.41%), Query Frame = 0


Query: 15  EPVAVIFGVTGLVGKELAKTLL-STGGWKVYGVARRPDNVSPITDSNFQFISCDLLDRSS 74
           E VA+IFGVTGLVG+E+ KTLL S  GW++YGVAR P+ ++ +T   + FISCDLL+ S 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSKPGWRIYGVARNPE-INSMT-KMYNFISCDLLNASE 75

Query: 75  AHRNLSPVRH-VTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSLQT 134
             + LSP++  V+H+FWVTW+ +   DT +C  QN+TML N LD ILP A  L+H SLQT
Sbjct: 76  TKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSLQT 135

Query: 135 GIKHYVSL-----TGGGGGRMAVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSV 194
           G+KHYVSL       G G  +  Y E+ PR  SG NFYY LEDLL+E+++     V WSV
Sbjct: 136 GMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS--VVWSV 195

Query: 195 LRPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQ 254
            RPGL++G S+RT +NFMGSLCVYGA+CK L  PFVFGG+  CWE   I+GSD+ LVAEQ
Sbjct: 196 QRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLVAEQ 255

Query: 255 HVWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAEDEEGMFSPEFHYTAAM 314
           H++AAT+       GEAFN  NG  FTWKEIW  + +K GV   +E  MF   F +   M
Sbjct: 256 HIFAATSG-KVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQV-NETTMFDEGFWFGREM 315

Query: 315 AEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLD 374
            E+  VW EIV +E L+ T++E+LANW FLD+LFR P K++  R K DR GF  KY+ LD
Sbjct: 316 VERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTLD 375

Query: 375 SILYWIDVMRHDKLIP 384
           S+LYWIDVMR +KLIP
Sbjct: 376 SVLYWIDVMRDEKLIP 385

[Close]

BLAST of Cp4.1LG02g11730 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.8e-66
Identity = 156/376 (41.49%), Postives = 202/376 (53.72%), Query Frame = 0


Query: 15  EPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 26  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 85

Query: 75  RSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSL 134
                  LSP+  VTH+F+VTW    R   S+  + N +ML NVL  I+P A  LRHV L
Sbjct: 86  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 145

Query: 135 QTGIKHYVS-LTGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 194
           QTG KHY+   T   G R    + ED PR +   NFYY  ED+L E +      V WS+ 
Sbjct: 146 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTWSIH 205

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+AEQ 
Sbjct: 206 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIAEQQ 265

Query: 255 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAED-EEGMFSPEFHYTAAM 314
           +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     EEG           M
Sbjct: 266 IWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEG---KNLGLVEMM 325

Query: 315 AEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLD 374
             K +VW E+V+E  L    +EE+  W F D +  + G M+ S NKS   GF       +
Sbjct: 326 KGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSNN 385

Query: 375 SILYWIDVMRHDKLIP 384
           S + WID  +  K++P
Sbjct: 386 SFISWIDKYKAFKIVP 388

[Close]

BLAST of Cp4.1LG02g11730 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.8e-66
Identity = 156/376 (41.49%), Postives = 202/376 (53.72%), Query Frame = 0


Query: 15  EPVAVIFGVTGLVGKELAKTL----LSTGGWKVYGVARRPDNVSPITDSNFQFISCDLLD 74
           E VA+I GVTG+VG  LA+ L       G WKVYGVARRP   +   D    +I CD+ D
Sbjct: 25  ESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRP-RPTWNADHPIDYIQCDVSD 84

Query: 75  RSSAHRNLSPVRHVTHLFWVTWAAQLRFDTSDCSDQNRTMLANVLDVILPAASALRHVSL 134
                  LSP+  VTH+F+VTW    R   S+  + N +ML NVL  I+P A  LRHV L
Sbjct: 85  AEDTRSKLSPLTDVTHVFYVTWTN--RESESENCEANGSMLRNVLQAIIPYAPNLRHVCL 144

Query: 135 QTGIKHYVS-LTGGGGGRM-AVYCEDSPRAESGNNFYYALEDLLRERLSGGGRRVAWSVL 194
           QTG KHY+   T   G R    + ED PR +   NFYY  ED+L E +      V WS+ 
Sbjct: 145 QTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQI-QNFYYTQEDILFEEIK-KIETVTWSIH 204

Query: 195 RPGLILGCSNRTFFNFMGSLCVYGAICKKLKKPFVFGGSAACWEGFCINGSDARLVAEQH 254
           RP +I G S  +  N +G+LCVY AICK    P +F GS   WEGF +  SDA L+AEQ 
Sbjct: 205 RPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGF-MTASDADLIAEQQ 264

Query: 255 VWAATNEINTTADGEAFNVCNGWSFTWKEIWGAVAEKFGVAAED-EEGMFSPEFHYTAAM 314
           +WAA   ++  A  EAFN  N   F WK +W  +AE+FG+     EEG           M
Sbjct: 265 IWAA---VDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEG---KNLGLVEMM 324

Query: 315 AEKGKVWAEIVEEEGLIPTDMEELANWGFLDSLFRLPGKMVASRNKSDRLGFSVKYKMLD 374
             K +VW E+V+E  L    +EE+  W F D +  + G M+ S NKS   GF       +
Sbjct: 325 KGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEG-MIDSMNKSKEYGFLGFRNSNN 384

Query: 375 SILYWIDVMRHDKLIP 384
           S + WID  +  K++P
Sbjct: 385 SFISWIDKYKAFKIVP 387

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g11730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1C9CX66	1.6e-67	41.64	(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC...	[more]
Q9STX2	2.6e-65	41.49	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP...	[more]
A0A221J5X3	1.6e-62	40.48	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1...	[more]
Q6PQJ9	2.0e-62	40.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1	[more]
A0A221J5W8	2.0e-62	39.63	(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ...	[more]

BLAST of Cp4.1LG02g11730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525980.1	4.19e-291	100.00	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022948773.1	1.00e-279	95.91	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita moschata]	[more]
KAG6607304.1	5.81e-279	95.91	(S)-8-oxocitronellyl enol synthase CYC2, partial [Cucurbita argyrosperma subsp. ...	[more]
KAG7036983.1	1.17e-278	95.91	3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp...	[more]
XP_022998181.1	1.79e-278	95.42	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG02g11730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GA93	4.86e-280	95.91	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1K9J8	8.69e-279	95.42	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1I0N6	9.38e-232	82.10	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0LVI2	6.76e-231	80.76	Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G530150 P...	[more]
A0A6J1HM54	1.27e-229	81.33	3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita moschata OX=3662 GN=...	[more]

BLAST of Cp4.1LG02g11730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G58750.1	3.4e-113	54.79	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.1	1.8e-66	41.49	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24220.2	1.8e-66	41.49	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.720		coord: 16..383 e-value: 3.5E-102 score: 344.0
None	No IPR available	PANTHER	PTHR32487	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE	coord: 1..383
None	No IPR available	PANTHER	PTHR32487:SF12	3-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE ISOFORM X1	coord: 1..383
None	No IPR available	CDD	cd08948	5beta-POR_like_SDR_a	coord: 17..322 e-value: 1.92851E-99 score: 295.694
IPR001509	NAD-dependent epimerase/dehydratase	PFAM	PF01370	Epimerase	coord: 19..119 e-value: 1.1E-7 score: 31.6
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 16..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g11730.1	Cp4.1LG02g11730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity