Cp4.1LG02g10640 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g10640
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCp4.1LG02: 9390538 .. 9394618 (-)
RNA-Seq ExpressionCp4.1LG02g10640
SyntenyCp4.1LG02g10640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATAGAATAATAGTAGTACTCCAATTGCTAGGAAGTTGATAGACTCGTCATAAATTAGGTCGGGATCTTAGGATCGATGTCTTCAATGAGGTGCAAGTACTAGCCACTACCCTAATTTTCATGAGACTGACACGAAAAAAACTACAAGTAGTAGAAGCTAAAATTTAAAACACTGTGGTCAAGACGTAATAAAATAAAAGTTTATGTACCAAAAATTGATTTAGTCGTTAAAACAACCTAGTCTAAATTTTGACGTGGGCAAATTCGACCTCAAATCAATTAACAAAACTGTATGATCAAAACTACAAAAACAAAGATGAAAATTTTGTTGTTGTATATTGTTGATGTCCACAGCAACCGCCAAAATCGATGAGAATTGATTGAAAAAGCTGCATGCTTTAAAAAGTAGACAGGAATCATTTATTGCCCTGAAACGGCTTTGATTTAATTTAACCAAAGTTAACAAACAAACAAACAAACACACTCTACCCCATTACCATCATTTTTAATTCTCCTTCAAGTTTTCAACTGTAAAGTTGCTGTTTTGTTTCAATTTCTTCAATCTCTTCTCAATTATCATCATATTCCCAAATCAATTCTTGATGTCTGCCGTTATTATTCATGCCACCGAGTAATAAAACGTTGAGGCGATATGTAAACATGGGTTGTTAATTTCAGCGTTAAAAAGTCTTCCAAATTTTGAAACCCCAGCTCGGCCCAACCCCCTTTTTGTTTTTGTTTCCTCTCAATCTTCAACAACTCTCTTCTTTTCACCCGCTCCCGCTAACTAAACTCTCTACAAAATCATGGCGAAACAAAACCCCAGGAAAGGGGTGGACAGAGCGACCCATTTCTCGCCTTAGAATTCGTTTTCTTGAGATTTCTTGAGATTCCTTTCGATTTCTTGTGGGTTTGTCGAAAAAAAATTGTGGGTTTTGTGGGGATTTAGGTACTGAATAATGGGTAATAGCCTAATGTGCAAGTCAAAGAAGGACGTGAAAGAGAATGGATCTAGAAGTAAGAGAATAGGTCGGTCTCAGAGGAAGTTGCAGAGTGAAGAAGAGTATTTGCAGAAGCAAGCTCTTTCGTTGGCTCTTCATCAGCTCCAATTATCTCAGAGGTTCGATGGATCCACTTCGAAGAGAATTGGGTCGACTAGCTCTCGTAGACGCAATCTCTCCGATCCATTCTCTAATGGAAAGCAGGTGGGACGGACTTCATTTTTTGTCAACACTTTTGATTACTTATTTTGTGGGTGATTGAATCATTCAAGAAAAAGTGGGTTATCAGGATTATGCTCTGTTAATTTCATAGCCGATTGCCTTTTGGGAATCTCATATTTTATAATCATTCTTAGTCTACTTTTCAGTTTTAAATAGTTTTTTTCCCCTGCTTTTTATGGATTGACCTTTTCAGGCTATCATTACTATGTTCTTTATATCATTATGGGGAGGATGAATGATCTTTACTGCTTAATATAGATATCAGAAAACAACTTTTAGGCAATTAATCTGCCATGTGATGCAAATTATGAGAAATCTACTAGCCTTGAATGGAAAGTAAAAGGACTATTGAAATGTAATCAGTATAATCTTAATGAGTTTTTACCCCTCAGAGTCAGAGCAAGAGAGATTCAAAGTTGTGTGGAGCAGCAGATGTGAGTTCAAGTGAAGCAAATCTGCAGGTGAGGCTTGGAATGTTTACTTTTTCTCATTTTCATTCTTTGATTTGTTGTTCATGAGTGAGTTTTTCATTGATGGATCTATCATATAATTTGACACAGGCAGCAGATTTTCTGGAAAATGTGAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGCTGGTACAAAACCATTTCTCTACTAGAGGAATCTGGATTGTCGCCCGTAGCCATTGATCTCAAAGGTTCCGGTATCGATCTAACCGATACAAACGGGGTGAATACTCTAGCAGAGTATTCAAAGCCATTGACTGATTATCTTCAAGATCTTCCTAATGATGAAAAGGTTGGATAACAAATAGTTCAGGATTGTTTGTTTGTGAGTTTAGTTTAAAGATTTATCTTCAAAAAAATTGTTCTGATGGTTCAACTGCTTGTTCTCCAAATTAGACTGCATAACTAAGATGGATAGTACGATACTTGTTAGCGTGTCCGTGTCAGTGTCCGTGTCTTGTATCTGTATCTATACTTCATAGTTACTTAACGATTCTTGTATTTTGAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTCTTTCGTATGCATCGGAATATTTCTCGAATAAAATAGCGAAAGCAATTTACGTGTGTGCAACGATGGTGGCTGATGGACAGAGACCTTTTGATGTATTCATGGAAGAGGTAAGTGATTAGCATTTATAGCTATGGAAAGTTGCTTTTCTGGGCATTTTAAGAACATTTCCTCTCAGTTGTTCGTGTTGATGATATAAACTTATGATCGTTTTGCAGATTGGATCAAAAGAGATTTTTATGAAAGACTCTAAGAATTTGATGTATGGGAAGGGAAAAGACAAACCTCCTACAGGATTCATGTTTGAGAAAGAACAAATGAAGGGGTTGTACTTTAATCAATCTCCTACAAAGGTAGTTTAGTTTAGATCATTACTTAGTGTGTGTGATTCATTATTAATGTGTGGTATCACTGCAGGATGTTGCTTTGGCTATGGTTTCCATGAGACCTTTCCCACTGGGTCCAGTCATGGAGAAGCTCTCACTGTCGCCCCAAAACTATGGAAGCGGGCGTCGATTCTTCGTTCAAACGCTCGACGATCGTGCACTCTCACCTGATGTTCAAGAAAGGCTGGTGAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGTCCTTTTTTCTCTAAGCCACAGTCACTGCACAAGATCCTCCTGGAAATCGCTCAAATACCATAAAAACTAGGCCTCTCTTACTCAAATCTGTCTAACAGAAAGAACAAAAATATTTTTTTTTTTTCTCTTTTTCCTTTTGAAGTTACTTATTTATAGCAGAACCAAACAAATTGTGGATGGAAATCAGTCATGTTCTGGTGTTAGAAGTTCTAGTGATATATAAAGAACCATAATTTTTCTTGTAAAGATACGAAGTGTTGGATTAGAGTTTCATTCATGTCGATTTTGAAGCAATAAGAAGGCATCTGGTTCATGAATTTAACATAATAATCTGCAAATCTAATTGAATGAGTTGAATATGAGCAGAAAGAATAAATAAAAGTATTATATATTAGTCGACAGAAACACATATCATAATCATCCATCACAATGCCCATCTACGACATGAAAGAGTATAAGTTTGATGTTTCTGATTCACTGCCCCACGCATGGAATCGAAGAACAAATGTGTACCAAACCAAACACCCTTGTGAGATTATGGTAAGTACAGTACTACTAGGTACTTTCTACATTCAGCTTCTTCTAAGGGAAAGAAAACTTGGCATATGACCAAAACATGAAGCAACGGGAAAGTTGGAAGGAACCTCCCTCTTGGCTTTGATATCCTGTCACTCAAACCACGTATGTGTATATACCGACACCGACACGTTCGTCCAGGTGAAGCAATCACACTTGGTATGTTGGGCAAAAAATCAGATCATACCAAACCAAAATCCACAACCATATTTGGTCTTCAACCAATGAAACTTTGATTTCGTAAAACCAAAAATTCTAAGAACGCATGCATATTCGATCTTCAACCAGAGAACTTTGTGTTTGTTTTGTTACCACCTGTATACGACAAAACAATTGCAAATCTTGCAATCCAGTTCACGAAACCTATTACTGTTACCAACTGCTTCCAGGGCATATCTCCATTTTACATGGTGAATGCCCTCTGAGGATGTGCATTTTTTAATCCTCAGAAATGGAGACAATCCCCCTGGAAGTTTGAGGAACCATAGTCCTGTCTGGATGCCATTATCTGTTCGACAGATTACACACATCGTCATCGTCCAATGGAGAACCATGTCATAATCTTGGTTTTAGTCTTAAAGGAGATCAAATGTTTTGCTAAT

mRNA sequence

ATAATAGAATAATAGTAGTACTCCAATTGCTAGGAAGTTGATAGACTCGTCATAAATTAGGTCGGGATCTTAGGATCGATGTCTTCAATGAGGTGCAAGTACTAGCCACTACCCTAATTTTCATGAGACTGACACGAAAAAAACTACAAGTAGTAGAAGCTAAAATTTAAAACACTGTGGTCAAGACGTAATAAAATAAAAGTTTATGTACCAAAAATTGATTTAGTCGTTAAAACAACCTAGTCTAAATTTTGACGTGGGCAAATTCGACCTCAAATCAATTAACAAAACTGTATGATCAAAACTACAAAAACAAAGATGAAAATTTTGTTGTTGTATATTGTTGATGTCCACAGCAACCGCCAAAATCGATGAGAATTGATTGAAAAAGCTGCATGCTTTAAAAAGTAGACAGGAATCATTTATTGCCCTGAAACGGCTTTGATTTAATTTAACCAAAGTTAACAAACAAACAAACAAACACACTCTACCCCATTACCATCATTTTTAATTCTCCTTCAAGTTTTCAACTGTAAAGTTGCTGTTTTGTTTCAATTTCTTCAATCTCTTCTCAATTATCATCATATTCCCAAATCAATTCTTGATGTCTGCCGTTATTATTCATGCCACCGAGTAATAAAACGTTGAGGCGATATGTAAACATGGGTTGTTAATTTCAGCGTTAAAAAGTCTTCCAAATTTTGAAACCCCAGCTCGGCCCAACCCCCTTTTTGTTTTTGTTTCCTCTCAATCTTCAACAACTCTCTTCTTTTCACCCGCTCCCGCTAACTAAACTCTCTACAAAATCATGGCGAAACAAAACCCCAGGAAAGGGGTGGACAGAGCGACCCATTTCTCGCCTTAGAATTCGTTTTCTTGAGATTTCTTGAGATTCCTTTCGATTTCTTGTGGGTTTGTCGAAAAAAAATTGTGGGTTTTGTGGGGATTTAGGTACTGAATAATGGGTAATAGCCTAATGTGCAAGTCAAAGAAGGACGTGAAAGAGAATGGATCTAGAAGTAAGAGAATAGGTCGGTCTCAGAGGAAGTTGCAGAGTGAAGAAGAGTATTTGCAGAAGCAAGCTCTTTCGTTGGCTCTTCATCAGCTCCAATTATCTCAGAGGTTCGATGGATCCACTTCGAAGAGAATTGGGTCGACTAGCTCTCGTAGACGCAATCTCTCCGATCCATTCTCTAATGGAAAGCAGAGTCAGAGCAAGAGAGATTCAAAGTTGTGTGGAGCAGCAGATGTGAGTTCAAGTGAAGCAAATCTGCAGGCAGCAGATTTTCTGGAAAATGTGAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGCTGGTACAAAACCATTTCTCTACTAGAGGAATCTGGATTGTCGCCCGTAGCCATTGATCTCAAAGGTTCCGGTATCGATCTAACCGATACAAACGGGGTGAATACTCTAGCAGAGTATTCAAAGCCATTGACTGATTATCTTCAAGATCTTCCTAATGATGAAAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTCTTTCGTATGCATCGGAATATTTCTCGAATAAAATAGCGAAAGCAATTTACGTGTGTGCAACGATGGTGGCTGATGGACAGAGACCTTTTGATGTATTCATGGAAGAGATTGGATCAAAAGAGATTTTTATGAAAGACTCTAAGAATTTGATGTATGGGAAGGGAAAAGACAAACCTCCTACAGGATTCATGTTTGAGAAAGAACAAATGAAGGGGTTGTACTTTAATCAATCTCCTACAAAGGATGTTGCTTTGGCTATGGTTTCCATGAGACCTTTCCCACTGGGTCCAGTCATGGAGAAGCTCTCACTGTCGCCCCAAAACTATGGAAGCGGGCGTCGATTCTTCGTTCAAACGCTCGACGATCGTGCACTCTCACCTGATGTTCAAGAAAGGCTGGTGAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGTCCTTTTTTCTCTAAGCCACAGTCACTGCACAAGATCCTCCTGGAAATCGCTCAAATACCATAAAAACTAGGCCTCTCTTACTCAAATCTGTCTAACAGAAAGAACAAAAATATTTTTTTTTTTTCTCTTTTTCCTTTTGAAGTTACTTATTTATAGCAGAACCAAACAAATTGTGGATGGAAATCAGTCATGTTCTGGTGTTAGAAGTTCTAGTGATATATAAAGAACCATAATTTTTCTTGTAAAGATACGAAGTGTTGGATTAGAGTTTCATTCATGTCGATTTTGAAGCAATAAGAAGGCATCTGGTTCATGAATTTAACATAATAATCTGCAAATCTAATTGAATGAGTTGAATATGAGCAGAAAGAATAAATAAAAGTATTATATATTAGTCGACAGAAACACATATCATAATCATCCATCACAATGCCCATCTACGACATGAAAGAGTATAAGTTTGATGTTTCTGATTCACTGCCCCACGCATGGAATCGAAGAACAAATGTGTACCAAACCAAACACCCTTGTGAGATTATGGTAAGTACAGTACTACTAGGTACTTTCTACATTCAGCTTCTTCTAAGGGAAAGAAAACTTGGCATATGACCAAAACATGAAGCAACGGGAAAGTTGGAAGGAACCTCCCTCTTGGCTTTGATATCCTGTCACTCAAACCACGTATGTGTATATACCGACACCGACACGTTCGTCCAGGTGAAGCAATCACACTTGGTATGTTGGGCAAAAAATCAGATCATACCAAACCAAAATCCACAACCATATTTGGTCTTCAACCAATGAAACTTTGATTTCGTAAAACCAAAAATTCTAAGAACGCATGCATATTCGATCTTCAACCAGAGAACTTTGTGTTTGTTTTGTTACCACCTGTATACGACAAAACAATTGCAAATCTTGCAATCCAGTTCACGAAACCTATTACTGTTACCAACTGCTTCCAGGGCATATCTCCATTTTACATGGTGAATGCCCTCTGAGGATGTGCATTTTTTAATCCTCAGAAATGGAGACAATCCCCCTGGAAGTTTGAGGAACCATAGTCCTGTCTGGATGCCATTATCTGTTCGACAGATTACACACATCGTCATCGTCCAATGGAGAACCATGTCATAATCTTGGTTTTAGTCTTAAAGGAGATCAAATGTTTTGCTAAT

Coding sequence (CDS)

ATGGGTAATAGCCTAATGTGCAAGTCAAAGAAGGACGTGAAAGAGAATGGATCTAGAAGTAAGAGAATAGGTCGGTCTCAGAGGAAGTTGCAGAGTGAAGAAGAGTATTTGCAGAAGCAAGCTCTTTCGTTGGCTCTTCATCAGCTCCAATTATCTCAGAGGTTCGATGGATCCACTTCGAAGAGAATTGGGTCGACTAGCTCTCGTAGACGCAATCTCTCCGATCCATTCTCTAATGGAAAGCAGAGTCAGAGCAAGAGAGATTCAAAGTTGTGTGGAGCAGCAGATGTGAGTTCAAGTGAAGCAAATCTGCAGGCAGCAGATTTTCTGGAAAATGTGAAGGAAAAGAAATTTGTTTTAGTTCATGGTGAAGGGTTTGGAGCTTGGTGCTGGTACAAAACCATTTCTCTACTAGAGGAATCTGGATTGTCGCCCGTAGCCATTGATCTCAAAGGTTCCGGTATCGATCTAACCGATACAAACGGGGTGAATACTCTAGCAGAGTATTCAAAGCCATTGACTGATTATCTTCAAGATCTTCCTAATGATGAAAAGGTTGTTCTAGTTGGGCACAGTAGTGGAGGTGCTTGTCTTTCGTATGCATCGGAATATTTCTCGAATAAAATAGCGAAAGCAATTTACGTGTGTGCAACGATGGTGGCTGATGGACAGAGACCTTTTGATGTATTCATGGAAGAGATTGGATCAAAAGAGATTTTTATGAAAGACTCTAAGAATTTGATGTATGGGAAGGGAAAAGACAAACCTCCTACAGGATTCATGTTTGAGAAAGAACAAATGAAGGGGTTGTACTTTAATCAATCTCCTACAAAGGATGTTGCTTTGGCTATGGTTTCCATGAGACCTTTCCCACTGGGTCCAGTCATGGAGAAGCTCTCACTGTCGCCCCAAAACTATGGAAGCGGGCGTCGATTCTTCGTTCAAACGCTCGACGATCGTGCACTCTCACCTGATGTTCAAGAAAGGCTGGTGAGGGAAAACCCACCTGAAAGAGTTTTCAAGATCAAAGGCAGTGATCATTGTCCTTTTTTCTCTAAGCCACAGTCACTGCACAAGATCCTCCTGGAAATCGCTCAAATACCATAA

Protein sequence

MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQIP
Homology
BLAST of Cp4.1LG02g10640 vs. ExPASy Swiss-Prot
Match: Q9FVW3 (Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES14 PE=1 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 3.6e-141
Identity = 253/371 (68.19%), Postives = 297/371 (80.05%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKE---NGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDG 60
           MGN ++   KKD K+    GS+SKR+ RSQRKL ++EE L ++ALS+A+HQ QLSQRFDG
Sbjct: 1   MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDG 60

Query: 61  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 120
           S S+R+GSTS+R+R LSDPFSNGK                       Q  DF E++  KK
Sbjct: 61  SMSRRVGSTSTRKRTLSDPFSNGK-----------------------QVPDFSESLIVKK 120

Query: 121 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 180
           FVLVHGEGFGAWCWYK ++ LEESGLSPV +DL G G ++TDTN V+TL EYSKPL D L
Sbjct: 121 FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLL 180

Query: 181 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 240
           ++LP +EKV+LVGHS+GGA +SYA E F  KI+KAI+VCATMV+DGQRPFDVF EE+GS 
Sbjct: 181 ENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSA 240

Query: 241 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 300
           E FMK+S+ L+YG GKDKPPTGFMFEK  MKGLYFNQSP KD+ALAM+SMRP PLGP+ME
Sbjct: 241 ERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMME 300

Query: 301 KLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSL 360
           K+SL+ + YG GRRF+VQTLDDRALSPDVQE+LVREN PE VFKIKGSDHCPFFSKPQSL
Sbjct: 301 KVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSL 348

Query: 361 HKILLEIAQIP 369
           HKILLEIAQIP
Sbjct: 361 HKILLEIAQIP 348

BLAST of Cp4.1LG02g10640 vs. ExPASy Swiss-Prot
Match: Q940H7 (Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES12 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.0e-140
Identity = 253/372 (68.01%), Postives = 296/372 (79.57%), Query Frame = 0

Query: 1   MGNSLMCKSKKDV----KENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFD 60
           MGN ++C  KKDV      +GSRSKR+ RSQRKL ++EE L ++ALS+A+HQ Q+SQRFD
Sbjct: 1   MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFD 60

Query: 61  GSTSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEK 120
           GS S+RIGSTSSRR  LSD FSN K                       Q  +FLE++K K
Sbjct: 61  GSMSRRIGSTSSRRGTLSDSFSNNK-----------------------QVPEFLESLKVK 120

Query: 121 KFVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDY 180
           KFVLVHGEGFGAWCWYKTI+ LEESGLSPV +DL GSG ++TD N V+TL EYSKPL + 
Sbjct: 121 KFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIEL 180

Query: 181 LQDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGS 240
           +Q+LP +EKV+LVGHS+GGAC+SYA E F  KI+KAI++CATMV DGQRPFDVF +E+GS
Sbjct: 181 IQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVTDGQRPFDVFADELGS 240

Query: 241 KEIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVM 300
            E FMK+S+ L+YG GKD P TGFMFEK+ MKGLYFNQSP KD+AL+M+SMRP PLGP+M
Sbjct: 241 AERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMM 300

Query: 301 EKLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQS 360
           EKLSLS + YG GRRF+VQTLDD ALSPDVQE+LVREN PE VFKIKGSDHCPFFSKPQS
Sbjct: 301 EKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQS 349

Query: 361 LHKILLEIAQIP 369
           LHKILLEIAQIP
Sbjct: 361 LHKILLEIAQIP 349

BLAST of Cp4.1LG02g10640 vs. ExPASy Swiss-Prot
Match: Q9FW03 (Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES11 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 1.0e-79
Identity = 162/352 (46.02%), Postives = 228/352 (64.77%), Query Frame = 0

Query: 22  RIGRSQRKLQSEEEYLQKQALSLA--LHQLQL----SQRFDGSTSKRIGSTSSRRRNLSD 81
           R   S+R  + E+  +Q+ AL+ A  L + Q     S  FD S S+R   + S++  L  
Sbjct: 46  RSSSSRRDNKFEDALIQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQL-- 105

Query: 82  PFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEGFGAWCWYKTI 141
           P S+  +S+S  D  L            L     L++++   FVLVHG  FGAWCWYKTI
Sbjct: 106 PRSSSSRSRSSTDPLL-------QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTI 165

Query: 142 SLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEKVVLVGHSSGG 201
           +LLEE G    AIDL G GI+  + NG+ +L++Y KPLTD L+ LP  EKV+LVGH  GG
Sbjct: 166 ALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGG 225

Query: 202 ACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIFMKDSKNLMYGKGKDK 261
           AC+SYA E F +KI+KA+++ A M+ +GQ   D+F  + G  ++ M+ ++  +Y  G + 
Sbjct: 226 ACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQNDL-MRKAQIFIYTNGNEN 285

Query: 262 PPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQNYGSGRRFFVQ 321
           PPT    +K  +K L FNQSP+KDVALA VSMR  P  PV+EKLSLS  NYGS RR++++
Sbjct: 286 PPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIE 345

Query: 322 TLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQI 368
           TL+D A+   +QE ++  +PPE+V+++KG+DH PFFSKPQ+LHK+LLEIA+I
Sbjct: 346 TLEDNAIPVTLQENMINSSPPEKVYRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Cp4.1LG02g10640 vs. ExPASy Swiss-Prot
Match: F4IE65 (Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES13 PE=2 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 147/311 (47.27%), Postives = 210/311 (67.52%), Query Frame = 0

Query: 58  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 117
           S++  + S+SSR+R+ +DP     Q   K  +K                   +E  + K+
Sbjct: 149 SSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNK-------------------VEGSETKR 208

Query: 118 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 177
           FVLVHG GFGAWCWYKTI+LLE+ G    A++L GSG+   DTN + +LA YSKPL  + 
Sbjct: 209 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFF 268

Query: 178 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 237
           + L   EKV+LVGH  GGAC+SYA E F  KIAKA+++ A M+A+GQ   D+F +++GS 
Sbjct: 269 ESLKPTEKVILVGHDFGGACMSYAMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSN 328

Query: 238 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 297
           ++ M+ ++  +Y  GK  PPT   F++  ++   FNQSP KD+ALA VS+RP P  PV E
Sbjct: 329 DL-MQQAQIFLYANGKKNPPTAVDFDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSE 388

Query: 298 KLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSL 357
           K+ +S +NYGS RRF+++T++D A+   +QE +++ NPPE+VF++KGSDH PFFS+PQSL
Sbjct: 389 KVHVSEKNYGSIRRFYIKTMEDYAVPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSL 439

Query: 358 HKILLEIAQIP 369
           +KIL+EI+QIP
Sbjct: 449 NKILVEISQIP 439

BLAST of Cp4.1LG02g10640 vs. ExPASy Swiss-Prot
Match: F4I0K9 (Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES15 PE=2 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.8e-76
Identity = 145/312 (46.47%), Postives = 201/312 (64.42%), Query Frame = 0

Query: 58  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 117
           ST   + S+S+R+R+ +DP     Q   K                       +E  + K+
Sbjct: 148 STYGYVRSSSNRQRSSTDPVLKPNQLLDKELK--------------------VEGAETKR 207

Query: 118 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 177
           FVLVHG GFGAWCWYKTI+LLE+ G    A+DL GSG+   DTN + +LA+Y KPL  + 
Sbjct: 208 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFF 267

Query: 178 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 237
             L   EKV+LVGH  GGAC+SYA E + +KIAKAI++ A M+A+ Q   D+F ++  S 
Sbjct: 268 DTLKPTEKVILVGHDFGGACMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSN 327

Query: 238 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 297
              M+     +Y  GK  PPT   F++  ++  +FNQSP KDVALA VSMRP P  PV+E
Sbjct: 328 YDLMEQVHLFLYANGKKNPPTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVE 387

Query: 298 KLSLSPQNYGSGRRFFVQTL-DDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQS 357
           KL +S +NYGS RRF+++T+ DD A+   +Q+ +++ NPPE+VF +KGSDH PFFS+PQS
Sbjct: 388 KLHVSEKNYGSIRRFYIKTMEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQS 439

Query: 358 LHKILLEIAQIP 369
           L++IL+EI+Q+P
Sbjct: 448 LNRILVEISQLP 439

BLAST of Cp4.1LG02g10640 vs. NCBI nr
Match: XP_023523937.1 (putative methylesterase 12, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 719 bits (1856), Expect = 3.41e-261
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. NCBI nr
Match: KAG7037153.1 (putative methylesterase 12, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 717 bits (1850), Expect = 2.80e-260
Identity = 366/368 (99.46%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. NCBI nr
Match: XP_022973414.1 (putative methylesterase 12, chloroplastic [Cucurbita maxima])

HSP 1 Score: 713 bits (1840), Expect = 9.36e-259
Identity = 364/368 (98.91%), Postives = 366/368 (99.46%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADV SSEANLQAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVCSSEANLQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYG GRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGRGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. NCBI nr
Match: XP_022948758.1 (putative methylesterase 12, chloroplastic [Cucurbita moschata])

HSP 1 Score: 713 bits (1840), Expect = 9.36e-259
Identity = 364/368 (98.91%), Postives = 367/368 (99.73%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEAN QAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANPQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYGSGRRFFVQTLDDRALSPDVQE+LVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. NCBI nr
Match: KAG6607494.1 (putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 707 bits (1825), Expect = 1.50e-256
Identity = 361/363 (99.45%), Postives = 363/363 (100.00%), Query Frame = 0

Query: 6   MCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTSKRIGS 65
           MCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTSKRIGS
Sbjct: 1   MCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTSKRIGS 60

Query: 66  TSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEG 125
           TSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEG
Sbjct: 61  TSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEG 120

Query: 126 FGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEK 185
           FGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEK
Sbjct: 121 FGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEK 180

Query: 186 VVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIFMKDSK 245
           VVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIFMKDSK
Sbjct: 181 VVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIFMKDSK 240

Query: 246 NLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQN 305
           NLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQN
Sbjct: 241 NLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQN 300

Query: 306 YGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIA 365
           YGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIA
Sbjct: 301 YGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIA 360

Query: 366 QIP 368
           QIP
Sbjct: 361 QIP 363

BLAST of Cp4.1LG02g10640 vs. ExPASy TrEMBL
Match: A0A6J1IEI4 (putative methylesterase 12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111471963 PE=4 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 4.53e-259
Identity = 364/368 (98.91%), Postives = 366/368 (99.46%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADV SSEANLQAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVCSSEANLQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYG GRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGRGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. ExPASy TrEMBL
Match: A0A6J1GA79 (putative methylesterase 12, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111452332 PE=4 SV=1)

HSP 1 Score: 713 bits (1840), Expect = 4.53e-259
Identity = 364/368 (98.91%), Postives = 367/368 (99.73%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS
Sbjct: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEAN QAADFLENVKEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANPQAADFLENVKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           PNDEKVVLVGHSSGGACLSYASE+FSNKIAKA+YVCATMVADGQRPFDVFMEEIGSKEIF
Sbjct: 181 PNDEKVVLVGHSSGGACLSYASEHFSNKIAKAVYVCATMVADGQRPFDVFMEEIGSKEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSPQNYGSGRRFFVQTLDDRALSPDVQE+LVRENPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQEKLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 368

BLAST of Cp4.1LG02g10640 vs. ExPASy TrEMBL
Match: A0A1S3CA81 (putative methylesterase 12, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498713 PE=4 SV=1)

HSP 1 Score: 650 bits (1676), Expect = 3.98e-234
Identity = 334/368 (90.76%), Postives = 352/368 (95.65%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSL+CKSKKDVKE GSRSKR+GRSQRKLQSEEEYLQKQALSLAL QLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEYLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQ   K+DSKLCGA DV+S EAN+QA +FLEN+KEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQG--KKDSKLCGA-DVASGEANVQAPEFLENLKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEE GLSP+AIDLKGSGIDLTDTN VNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNRVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           P+DEKVVLVGHSSGGACLSYA E+FSNKI+KAIYVCATMVA GQRPFDVFMEE+GS+EIF
Sbjct: 181 PDDEKVVLVGHSSGGACLSYALEHFSNKISKAIYVCATMVATGQRPFDVFMEELGSEEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSK L+YG GKDKPPTGFMFEKEQ+KGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS
Sbjct: 241 MKDSKFLIYGNGKDKPPTGFMFEKEQIKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSP+NYG+GRRFFVQTLDD ALSPDVQE+LVR NPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPENYGTGRRFFVQTLDDHALSPDVQEKLVRVNPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 365

BLAST of Cp4.1LG02g10640 vs. ExPASy TrEMBL
Match: A0A0A0KUW5 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650640 PE=4 SV=1)

HSP 1 Score: 649 bits (1674), Expect = 8.03e-234
Identity = 334/368 (90.76%), Postives = 351/368 (95.38%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGNSL+CKSKKDVKE GSRSKR+GRSQRKLQSEEEYLQKQALSLAL QLQLSQRFDGSTS
Sbjct: 1   MGNSLICKSKKDVKEIGSRSKRMGRSQRKLQSEEEYLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KRIGSTSSRRRNLSDPFSNGKQ   K+DSKLCGA DV+S EANLQA DF+EN+KEKKFVL
Sbjct: 61  KRIGSTSSRRRNLSDPFSNGKQG--KKDSKLCGA-DVASGEANLQAPDFVENLKEKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           VHGEGFGAWCWYKTISLLEE GLSP+AIDLKGSGIDLTDTN VNTLAEYSKPLTDYLQDL
Sbjct: 121 VHGEGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNRVNTLAEYSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           P+DEKVVLVGHSSGGACLSYA E+FSNKI+KAIYVCATMVA GQRPFDVFMEE+GS+EIF
Sbjct: 181 PDDEKVVLVGHSSGGACLSYALEHFSNKISKAIYVCATMVATGQRPFDVFMEELGSEEIF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSK L+YG GKDKPPTGFMFEKEQ+KGLYFNQSPTKDVALAMVSMRPFPLGPVMEKL 
Sbjct: 241 MKDSKFLIYGNGKDKPPTGFMFEKEQIKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLL 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSP+NYG+GRRFFVQTLDD ALSPDVQE+LVR NPPERVFKIKGSDHCPFFSKPQSLHKI
Sbjct: 301 LSPENYGTGRRFFVQTLDDHALSPDVQEKLVRVNPPERVFKIKGSDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 365

BLAST of Cp4.1LG02g10640 vs. ExPASy TrEMBL
Match: A0A6J1D9J3 (putative methylesterase 12, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018287 PE=4 SV=1)

HSP 1 Score: 642 bits (1657), Expect = 3.36e-231
Identity = 324/368 (88.04%), Postives = 349/368 (94.84%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDGSTS 60
           MGN L+CKSKKDV ENGS+S+R+GRSQRKLQSEEE+LQKQALSLAL QLQLSQRFDGSTS
Sbjct: 1   MGNRLICKSKKDVNENGSKSRRMGRSQRKLQSEEEFLQKQALSLALQQLQLSQRFDGSTS 60

Query: 61  KRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVL 120
           KR+GSTSSRRRNLSDPFSNGKQSQSKRDSKL GA DV+S EANLQA +FLEN+K KKFVL
Sbjct: 61  KRVGSTSSRRRNLSDPFSNGKQSQSKRDSKLYGA-DVTSGEANLQAPEFLENLKAKKFVL 120

Query: 121 VHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDL 180
           +HGEGFGAWCWYKTISLLEE GLSP+AIDLKGSGIDLTDTN VNTLAE+SKPLTDYLQDL
Sbjct: 121 IHGEGFGAWCWYKTISLLEEVGLSPIAIDLKGSGIDLTDTNRVNTLAEFSKPLTDYLQDL 180

Query: 181 PNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIF 240
           P+DEKVVLVGHSSGGACLSYA E+F NKI+KAIY+CATMVA GQRPFDVFM+E+GS+E F
Sbjct: 181 PDDEKVVLVGHSSGGACLSYALEHFPNKISKAIYICATMVATGQRPFDVFMDELGSEETF 240

Query: 241 MKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLS 300
           MKDSK  +YG GKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGP+MEKLS
Sbjct: 241 MKDSKLFIYGNGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPIMEKLS 300

Query: 301 LSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKI 360
           LSP+NYG+GRRFF+QTLDD ALSPDVQE+LVRENPPERVFKIK SDHCPFFSKPQSLHKI
Sbjct: 301 LSPKNYGTGRRFFIQTLDDHALSPDVQEKLVRENPPERVFKIKASDHCPFFSKPQSLHKI 360

Query: 361 LLEIAQIP 368
           LLEIAQIP
Sbjct: 361 LLEIAQIP 367

BLAST of Cp4.1LG02g10640 vs. TAIR 10
Match: AT1G33990.1 (methyl esterase 14 )

HSP 1 Score: 502.7 bits (1293), Expect = 2.6e-142
Identity = 253/371 (68.19%), Postives = 297/371 (80.05%), Query Frame = 0

Query: 1   MGNSLMCKSKKDVKE---NGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFDG 60
           MGN ++   KKD K+    GS+SKR+ RSQRKL ++EE L ++ALS+A+HQ QLSQRFDG
Sbjct: 1   MGNKIISMMKKDSKDGGGGGSKSKRMNRSQRKLLADEEMLHRRALSMAIHQAQLSQRFDG 60

Query: 61  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 120
           S S+R+GSTS+R+R LSDPFSNGK                       Q  DF E++  KK
Sbjct: 61  SMSRRVGSTSTRKRTLSDPFSNGK-----------------------QVPDFSESLIVKK 120

Query: 121 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 180
           FVLVHGEGFGAWCWYK ++ LEESGLSPV +DL G G ++TDTN V+TL EYSKPL D L
Sbjct: 121 FVLVHGEGFGAWCWYKMVASLEESGLSPVTVDLTGCGFNMTDTNTVSTLEEYSKPLIDLL 180

Query: 181 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 240
           ++LP +EKV+LVGHS+GGA +SYA E F  KI+KAI+VCATMV+DGQRPFDVF EE+GS 
Sbjct: 181 ENLPEEEKVILVGHSTGGASISYALERFPEKISKAIFVCATMVSDGQRPFDVFSEELGSA 240

Query: 241 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 300
           E FMK+S+ L+YG GKDKPPTGFMFEK  MKGLYFNQSP KD+ALAM+SMRP PLGP+ME
Sbjct: 241 ERFMKESQFLIYGNGKDKPPTGFMFEKPHMKGLYFNQSPNKDIALAMISMRPVPLGPMME 300

Query: 301 KLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSL 360
           K+SL+ + YG GRRF+VQTLDDRALSPDVQE+LVREN PE VFKIKGSDHCPFFSKPQSL
Sbjct: 301 KVSLTAERYGKGRRFYVQTLDDRALSPDVQEKLVRENSPEGVFKIKGSDHCPFFSKPQSL 348

Query: 361 HKILLEIAQIP 369
           HKILLEIAQIP
Sbjct: 361 HKILLEIAQIP 348

BLAST of Cp4.1LG02g10640 vs. TAIR 10
Match: AT4G09900.1 (methyl esterase 12 )

HSP 1 Score: 501.1 bits (1289), Expect = 7.4e-142
Identity = 253/372 (68.01%), Postives = 296/372 (79.57%), Query Frame = 0

Query: 1   MGNSLMCKSKKDV----KENGSRSKRIGRSQRKLQSEEEYLQKQALSLALHQLQLSQRFD 60
           MGN ++C  KKDV      +GSRSKR+ RSQRKL ++EE L ++ALS+A+HQ Q+SQRFD
Sbjct: 1   MGNRVICMKKKDVVIRSGGDGSRSKRVNRSQRKLLADEENLHRRALSMAIHQAQVSQRFD 60

Query: 61  GSTSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEK 120
           GS S+RIGSTSSRR  LSD FSN K                       Q  +FLE++K K
Sbjct: 61  GSMSRRIGSTSSRRGTLSDSFSNNK-----------------------QVPEFLESLKVK 120

Query: 121 KFVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDY 180
           KFVLVHGEGFGAWCWYKTI+ LEESGLSPV +DL GSG ++TD N V+TL EYSKPL + 
Sbjct: 121 KFVLVHGEGFGAWCWYKTIASLEESGLSPVTVDLAGSGFNMTDANSVSTLEEYSKPLIEL 180

Query: 181 LQDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGS 240
           +Q+LP +EKV+LVGHS+GGAC+SYA E F  KI+KAI++CATMV DGQRPFDVF +E+GS
Sbjct: 181 IQNLPAEEKVILVGHSTGGACVSYALERFPEKISKAIFICATMVTDGQRPFDVFADELGS 240

Query: 241 KEIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVM 300
            E FMK+S+ L+YG GKD P TGFMFEK+ MKGLYFNQSP KD+AL+M+SMRP PLGP+M
Sbjct: 241 AERFMKESQFLIYGNGKDNPATGFMFEKQHMKGLYFNQSPNKDIALSMISMRPVPLGPMM 300

Query: 301 EKLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQS 360
           EKLSLS + YG GRRF+VQTLDD ALSPDVQE+LVREN PE VFKIKGSDHCPFFSKPQS
Sbjct: 301 EKLSLSAERYGKGRRFYVQTLDDLALSPDVQEKLVRENSPEAVFKIKGSDHCPFFSKPQS 349

Query: 361 LHKILLEIAQIP 369
           LHKILLEIAQIP
Sbjct: 361 LHKILLEIAQIP 349

BLAST of Cp4.1LG02g10640 vs. TAIR 10
Match: AT3G29770.1 (methyl esterase 11 )

HSP 1 Score: 298.5 bits (763), Expect = 7.3e-81
Identity = 162/352 (46.02%), Postives = 228/352 (64.77%), Query Frame = 0

Query: 22  RIGRSQRKLQSEEEYLQKQALSLA--LHQLQL----SQRFDGSTSKRIGSTSSRRRNLSD 81
           R   S+R  + E+  +Q+ AL+ A  L + Q     S  FD S S+R   + S++  L  
Sbjct: 46  RSSSSRRDNKFEDALIQEHALAAAAVLFRQQNGGGGSLPFDRSASQRYQGSCSKKNQL-- 105

Query: 82  PFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKKFVLVHGEGFGAWCWYKTI 141
           P S+  +S+S  D  L            L     L++++   FVLVHG  FGAWCWYKTI
Sbjct: 106 PRSSSSRSRSSTDPLL-------QPHQFLNQGIKLDDLETNHFVLVHGGSFGAWCWYKTI 165

Query: 142 SLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYLQDLPNDEKVVLVGHSSGG 201
           +LLEE G    AIDL G GI+  + NG+ +L++Y KPLTD L+ LP  EKV+LVGH  GG
Sbjct: 166 ALLEEDGFKVTAIDLAGCGINSININGIASLSQYVKPLTDILEKLPIGEKVILVGHDFGG 225

Query: 202 ACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSKEIFMKDSKNLMYGKGKDK 261
           AC+SYA E F +KI+KA+++ A M+ +GQ   D+F  + G  ++ M+ ++  +Y  G + 
Sbjct: 226 ACISYAMELFPSKISKAVFLAAAMLTNGQSTLDMFSLKAGQNDL-MRKAQIFIYTNGNEN 285

Query: 262 PPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVMEKLSLSPQNYGSGRRFFVQ 321
           PPT    +K  +K L FNQSP+KDVALA VSMR  P  PV+EKLSLS  NYGS RR++++
Sbjct: 286 PPTAIDLDKSLLKDLLFNQSPSKDVALASVSMRSIPFAPVLEKLSLSDANYGSVRRYYIE 345

Query: 322 TLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSLHKILLEIAQI 368
           TL+D A+   +QE ++  +PPE+V+++KG+DH PFFSKPQ+LHK+LLEIA+I
Sbjct: 346 TLEDNAIPVTLQENMINSSPPEKVYRLKGADHAPFFSKPQALHKLLLEIARI 387

BLAST of Cp4.1LG02g10640 vs. TAIR 10
Match: AT1G26360.1 (methyl esterase 13 )

HSP 1 Score: 298.1 bits (762), Expect = 9.6e-81
Identity = 147/311 (47.27%), Postives = 210/311 (67.52%), Query Frame = 0

Query: 58  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 117
           S++  + S+SSR+R+ +DP     Q   K  +K                   +E  + K+
Sbjct: 149 SSAGFVRSSSSRQRSSTDPMIKPNQLVDKELNK-------------------VEGSETKR 208

Query: 118 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 177
           FVLVHG GFGAWCWYKTI+LLE+ G    A++L GSG+   DTN + +LA YSKPL  + 
Sbjct: 209 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVELTGSGVSSIDTNNITSLAHYSKPLLHFF 268

Query: 178 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 237
           + L   EKV+LVGH  GGAC+SYA E F  KIAKA+++ A M+A+GQ   D+F +++GS 
Sbjct: 269 ESLKPTEKVILVGHDFGGACMSYAMEMFPTKIAKAVFISAAMLANGQSTLDLFNQQLGSN 328

Query: 238 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 297
           ++ M+ ++  +Y  GK  PPT   F++  ++   FNQSP KD+ALA VS+RP P  PV E
Sbjct: 329 DL-MQQAQIFLYANGKKNPPTAVDFDRSLLRDFLFNQSPPKDLALASVSIRPIPFAPVSE 388

Query: 298 KLSLSPQNYGSGRRFFVQTLDDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQSL 357
           K+ +S +NYGS RRF+++T++D A+   +QE +++ NPPE+VF++KGSDH PFFS+PQSL
Sbjct: 389 KVHVSEKNYGSIRRFYIKTMEDYAVPVLLQEAMIKLNPPEQVFQLKGSDHAPFFSRPQSL 439

Query: 358 HKILLEIAQIP 369
           +KIL+EI+QIP
Sbjct: 449 NKILVEISQIP 439

BLAST of Cp4.1LG02g10640 vs. TAIR 10
Match: AT1G69240.1 (methyl esterase 15 )

HSP 1 Score: 287.7 bits (735), Expect = 1.3e-77
Identity = 145/312 (46.47%), Postives = 201/312 (64.42%), Query Frame = 0

Query: 58  STSKRIGSTSSRRRNLSDPFSNGKQSQSKRDSKLCGAADVSSSEANLQAADFLENVKEKK 117
           ST   + S+S+R+R+ +DP     Q   K                       +E  + K+
Sbjct: 148 STYGYVRSSSNRQRSSTDPVLKPNQLLDKELK--------------------VEGAETKR 207

Query: 118 FVLVHGEGFGAWCWYKTISLLEESGLSPVAIDLKGSGIDLTDTNGVNTLAEYSKPLTDYL 177
           FVLVHG GFGAWCWYKTI+LLE+ G    A+DL GSG+   DTN + +LA+Y KPL  + 
Sbjct: 208 FVLVHGGGFGAWCWYKTITLLEKHGFQVDAVDLTGSGVSSFDTNNITSLAQYVKPLLHFF 267

Query: 178 QDLPNDEKVVLVGHSSGGACLSYASEYFSNKIAKAIYVCATMVADGQRPFDVFMEEIGSK 237
             L   EKV+LVGH  GGAC+SYA E + +KIAKAI++ A M+A+ Q   D+F ++  S 
Sbjct: 268 DTLKPTEKVILVGHDFGGACMSYAMEMYPSKIAKAIFISAAMLANAQSTLDLFNQQPDSN 327

Query: 238 EIFMKDSKNLMYGKGKDKPPTGFMFEKEQMKGLYFNQSPTKDVALAMVSMRPFPLGPVME 297
              M+     +Y  GK  PPT   F++  ++  +FNQSP KDVALA VSMRP P  PV+E
Sbjct: 328 YDLMEQVHLFLYANGKKNPPTAVDFDRSLLRDFFFNQSPPKDVALASVSMRPIPFAPVVE 387

Query: 298 KLSLSPQNYGSGRRFFVQTL-DDRALSPDVQERLVRENPPERVFKIKGSDHCPFFSKPQS 357
           KL +S +NYGS RRF+++T+ DD A+   +Q+ +++ NPPE+VF +KGSDH PFFS+PQS
Sbjct: 388 KLHVSEKNYGSIRRFYIKTMEDDYAVPVSLQDAMIKSNPPEQVFHLKGSDHAPFFSRPQS 439

Query: 358 LHKILLEIAQIP 369
           L++IL+EI+Q+P
Sbjct: 448 LNRILVEISQLP 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FVW33.6e-14168.19Putative methylesterase 14, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q940H71.0e-14068.01Putative methylesterase 12, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Q9FW031.0e-7946.02Putative methylesterase 11, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4IE651.3e-7947.27Putative methylesterase 13, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
F4I0K91.8e-7646.47Putative methylesterase 15, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MES... [more]
Match NameE-valueIdentityDescription
XP_023523937.13.41e-261100.00putative methylesterase 12, chloroplastic [Cucurbita pepo subsp. pepo][more]
KAG7037153.12.80e-26099.46putative methylesterase 12, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
XP_022973414.19.36e-25998.91putative methylesterase 12, chloroplastic [Cucurbita maxima][more]
XP_022948758.19.36e-25998.91putative methylesterase 12, chloroplastic [Cucurbita moschata][more]
KAG6607494.11.50e-25699.45putative methylesterase 12, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
Match NameE-valueIdentityDescription
A0A6J1IEI44.53e-25998.91putative methylesterase 12, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1GA794.53e-25998.91putative methylesterase 12, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A1S3CA813.98e-23490.76putative methylesterase 12, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10349871... [more]
A0A0A0KUW58.03e-23490.76AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G650... [more]
A0A6J1D9J33.36e-23188.04putative methylesterase 12, chloroplastic OS=Momordica charantia OX=3673 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT1G33990.12.6e-14268.19methyl esterase 14 [more]
AT4G09900.17.4e-14268.01methyl esterase 12 [more]
AT3G29770.17.3e-8146.02methyl esterase 11 [more]
AT1G26360.19.6e-8147.27methyl esterase 13 [more]
AT1G69240.11.3e-7746.47methyl esterase 15 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 110..366
e-value: 4.2E-59
score: 202.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 118..364
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 118..355
e-value: 1.3E-17
score: 65.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..89
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 8..31
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 57..92
NoneNo IPR availablePANTHERPTHR10992:SF785METHYLESTERASE 14, CHLOROPLASTIC-RELATEDcoord: 25..367
NoneNo IPR availablePANTHERPTHR10992METHYLESTERASE FAMILY MEMBERcoord: 25..367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g10640.1Cp4.1LG02g10640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009694 jasmonic acid metabolic process
biological_process GO:0009696 salicylic acid metabolic process
molecular_function GO:0080030 methyl indole-3-acetate esterase activity
molecular_function GO:0080032 methyl jasmonate esterase activity
molecular_function GO:0080031 methyl salicylate esterase activity