Cp4.1LG02g09550 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g09550
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	ankyrin repeat-containing protein ITN1-like
Location	Cp4.1LG02: 8268837 .. 8269627 (+)
RNA-Seq Expression	Cp4.1LG02g09550
Synteny	Cp4.1LG02g09550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTGGGGTGTCCTTCCACAAAACAAGAAACTAAAACCCAAAGAAGCTATGAATGTGTACGAGGAGAAAAAACAGTTATACAAACTAGCGTTGAGGGGAGAATGGAGGCATCTGCGCGAGGTATGTAAGGATGCGAGGCAGTTAATGATGCCGATCAATAACAGTGGAGAGACGGCGTTGCATTTGGCAGTGCACAGTGGAAAAGAAGAACCTTTAAAAACAATTCTTGAGCAGTTAGTGTTGTCGATCAATAACAGTGGAGAGATGGATTACACAGAGCATTGGAAGAGCTCCGCTGAAAACACACCTCTACATGAAGCTGCCACTGCGGGGAATATGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAGTCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGGTCGTTGAATATATTTTGAATGAATGTGAAGATTATTACTCTCGATCTCCCCGAAATTGGATGGCAAAGGGTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGGTTAGTGTCTAAGTTTTTGTTACCATAACTGAAGCTAATTCAAAGCTTTAATGCCTAATTCAATGTTTTTATTGCAGAGATGGTTATGATGCTGCTGGATTTCGACAAGTCACTGTTGGAGATGAGGGATTCAAAAAAGCGGACGGCTCTCCACGTGCTGGCCAATATGCCACACATTTTTGAGAGTGGATACGTCAAGGGATTTTGGGTCGAATTGATATATAATTGTAACAAT

mRNA sequence

ATTTGGGGTGTCCTTCCACAAAACAAGAAACTAAAACCCAAAGAAGCTATGAATGTGTACGAGGAGAAAAAACAGTTATACAAACTAGCGTTGAGGGGAGAATGGAGGCATCTGCGCGAGGTATGTAAGGATGCGAGGCAGTTAATGATGCCGATCAATAACAGTGGAGAGACGGCGTTGCATTTGGCAGTGCACAGTGGAAAAGAAGAACCTTTAAAAACAATTCTTGAGCAGTTAGTGTTGTCGATCAATAACAGTGGAGAGATGGATTACACAGAGCATTGGAAGAGCTCCGCTGAAAACACACCTCTACATGAAGCTGCCACTGCGGGGAATATGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAGTCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGGTCGTTGAATATATTTTGAATGAATGTGAAGATTATTACTCTCGATCTCCCCGAAATTGGATGGCAAAGGGTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGAGATGGTTATGATGCTGCTGGATTTCGACAAGTCACTGTTGGAGATGAGGGATTCAAAAAAGCGGACGGCTCTCCACGTGCTGGCCAATATGCCACACATTTTTGAGAGTGGATACGTCAAGGGATTTTGGGTCGAATTGATATATAATTGTAACAAT

Coding sequence (CDS)

ATTTGGGGTGTCCTTCCACAAAACAAGAAACTAAAACCCAAAGAAGCTATGAATGTGTACGAGGAGAAAAAACAGTTATACAAACTAGCGTTGAGGGGAGAATGGAGGCATCTGCGCGAGGTATGTAAGGATGCGAGGCAGTTAATGATGCCGATCAATAACAGTGGAGAGACGGCGTTGCATTTGGCAGTGCACAGTGGAAAAGAAGAACCTTTAAAAACAATTCTTGAGCAGTTAGTGTTGTCGATCAATAACAGTGGAGAGATGGATTACACAGAGCATTGGAAGAGCTCCGCTGAAAACACACCTCTACATGAAGCTGCCACTGCGGGGAATATGGATGCTTTCAAGCTTTTGGTTGAGTTCAGGAAGGAGGATTTGTTGGAGTTGAACAGTCGGGGTGAAACGCCTTTGTATAGGGCAGCCAGATATGGGCAACTTCAGGTCGTTGAATATATTTTGAATGAATGTGAAGATTATTACTCTCGATCTCCCCGAAATTGGATGGCAAAGGGTACTCCAATCATTCATGAAGCCATTCAAAGCGAGAACTTTGAGATGGTTATGATGCTGCTGGATTTCGACAAGTCACTGTTGGAGATGAGGGATTCAAAAAAGCGGACGGCTCTCCACGTGCTGGCCAATATGCCACACATTTTTGAGAGTGGATACGTCAAGGGATTTTGGGTCGAATTGATATATAATTGTAACAAT

Protein sequence

IWGVLPQNKKLKPKEAMNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYNCNN

Homology

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot
Match: B1AK53 (Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.3e-11
Identity = 50/166 (30.12%), Postives = 73/166 (43.98%), Query Frame = 0


Query: 53  NNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGN 112
           +NSG T LHLA   G  E +  +L        + G  D T      A   P+H AA  G+
Sbjct: 101 DNSGATVLHLAARFGHPEVVNWLL--------HHGGGDPTAATDMGA--LPIHYAAAKGD 160

Query: 113 MDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKG 172
             + +LLVE   E +      G TPLY A + G L+V +Y++ EC       P      G
Sbjct: 161 FPSLRLLVEHYPEGVNAQTKNGATPLYLACQEGHLEVTQYLVQEC----GADPHARAHDG 220

Query: 173 TPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANMPH 219
              +H A Q  +  +++ L+      L  +D    TA+H  A+  H
Sbjct: 221 MTPLHAAAQMGHSPVIVWLVSCTDVSLSEQDKDGATAMHFAASRGH 252

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot
Match: Q9ET47 (Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2)

HSP 1 Score: 70.9 bits (172), Expect = 2.3e-11
Identity = 50/166 (30.12%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 53  NNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGN 112
           +NSG T LHLA   G  + +K +L Q     N++   D            P+H AA  G+
Sbjct: 101 DNSGATVLHLAARFGHPDVVKWLLYQ--GGANSAITTD--------TGALPIHYAAAKGD 160

Query: 113 MDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKG 172
           + + KLLV    E +    + G TPLY A + G L+V +Y++ EC    S  P      G
Sbjct: 161 LPSLKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----SADPHLRAQDG 220

Query: 173 TPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANMPH 219
              +H A Q  +  +++ L+ F       +D    TA+H  A+  H
Sbjct: 221 MTPLHAAAQMGHNPVLVWLVSFADVSFSEQDHDGATAMHFAASRGH 252

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot
Match: Q63618 (Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 2.5e-10
Identity = 50/166 (30.12%), Postives = 76/166 (45.78%), Query Frame = 0


Query: 53  NNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGN 112
           +NSG T LHLA   G  + +  +L Q     N++   D            P+H AA  G+
Sbjct: 101 DNSGATVLHLAARFGHPDVVNWLLYQ--GGANSAITTD--------TGALPIHYAAAKGD 160

Query: 113 MDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKG 172
           + + KLLV    E +    + G TPLY A + G L+V +Y++ EC    S  P      G
Sbjct: 161 LPSMKLLVGHYPEGVNAQTNNGATPLYLACQEGHLEVTKYLVQEC----SADPHLRAQDG 220

Query: 173 TPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANMPH 219
              +H A Q  +  +++ L+ F     E +D    TA+H  A+  H
Sbjct: 221 MTPLHAAAQMGHNPVLVWLVSFADVSFE-QDHDGATAMHFAASRGH 251

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot
Match: Q6AWW5 (Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=At5g02620 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 4.3e-10
Identity = 50/197 (25.38%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 44  DARQLMMPINNSGETALHLAVHSGKEEPLKTI---------------------------- 103
           + ++L+   N SGETAL++A   G  + +K +                            
Sbjct: 44  ELKELLAEQNQSGETALYVAAEYGYTDMVKILMKHSDSVLAGTKAKNGFDAFHIAAKNGN 103

Query: 104 LEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGE 163
           L+ L + I  + E+ +T     S++ T LH AA+ G+ +    L++   +      S G+
Sbjct: 104 LQVLDVLIEANPELSFT---FDSSKTTALHTAASQGHGEIVCFLLDKGVDLAAIARSNGK 163

Query: 164 TPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFD 213
           T L+ AAR G   +V+ ++ +     +R  +    KG   +H A++ +N E+V +L++ D
Sbjct: 164 TALHSAARNGHTVIVKKLIEKKAGMVTRVDK----KGQTALHMAVKGQNTEIVDVLMEAD 223

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot
Match: Q92625 (Ankyrin repeat and SAM domain-containing protein 1A OS=Homo sapiens OX=9606 GN=ANKS1A PE=1 SV=4)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 50/165 (30.30%), Postives = 79/165 (47.88%), Query Frame = 0


Query: 53  NNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGN 112
           NN  ETALH A   G  E +K +LE+L          D T   +++   TPL  AA  G 
Sbjct: 146 NNDNETALHCAAQYGHTEVVKVLLEELT---------DPT--MRNNKFETPLDLAALYGR 205

Query: 113 MDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKG 172
           ++  K+L+     +LL  N++  TPL+ AAR G   VV+ +L+   D       N+  + 
Sbjct: 206 LEVVKMLLN-AHPNLLSCNTKKHTPLHLAARNGHKAVVQVLLDAGMD------SNYQTEM 265

Query: 173 TPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANMP 218
              +HEA      ++V +LL      + ++D+   TAL  +  +P
Sbjct: 266 GSALHEAALFGKTDVVQILLAAGTD-VNIKDNHGLTALDTVRELP 291

[Close]

BLAST of Cp4.1LG02g09550 vs. NCBI nr
Match: KAG7012617.1 (Espin, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 413 bits (1061), Expect = 1.24e-138
Identity = 202/220 (91.82%), Postives = 210/220 (95.45%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQLVL INNSGEMDYTEHWKSSAENTPLHEAATAGN+DAFKLLVEFRKEDLLELN RGET
Sbjct: 61  EQLVLPINNSGEMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLELNKRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAK-GTPIIHEAIQSENFEMVMMLLDFD 196
           PLYRAARYGQL++VEYILNECEDYYSRSP NW+ + GTPII  AIQSENFEMVMMLLDFD
Sbjct: 121 PLYRAARYGQLEIVEYILNECEDYYSRSPLNWITREGTPIIQAAIQSENFEMVMMLLDFD 180

Query: 197 KSLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           KSLLEM+D K +TALHVLANMPHIFESGYVKGFW ELIY+
Sbjct: 181 KSLLEMKDPKNQTALHVLANMPHIFESGYVKGFWAELIYD 220

[Close]

BLAST of Cp4.1LG02g09550 vs. NCBI nr
Match: XP_022954414.1 (espin-like [Cucurbita moschata])

HSP 1 Score: 382 bits (982), Expect = 1.86e-132
Identity = 187/219 (85.39%), Postives = 200/219 (91.32%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           MNVYEEKKQLYKLALRGEWRHLREVCKDA QL++PINNSGETALHLAV+SGK EPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDASQLVLPINNSGETALHLAVYSGKGEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQ++       EMDY E+WKS  ENTPLHEAAT GN+DAFKLLVEFRKEDLLELN RGET
Sbjct: 61  EQVM-------EMDYIEYWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYGQLQ+VEYILNECEDYYSRSP NWM  GTPIIHEAIQSE+FEMVMML+DFDK
Sbjct: 121 PLYRAARYGQLQIVEYILNECEDYYSRSPLNWMVDGTPIIHEAIQSESFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW ELIYN
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWGELIYN 212

[Close]

BLAST of Cp4.1LG02g09550 vs. NCBI nr
Match: XP_022954460.1 (espin-like [Cucurbita moschata])

HSP 1 Score: 367 bits (942), Expect = 5.95e-126
Identity = 182/220 (82.73%), Postives = 197/220 (89.55%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M +YEEKKQLYKL LRGEWR L EVCK ARQL+ PI ++ +TA HLAV+SGK+EPLKTIL
Sbjct: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQ++       EMDYTEHWKSSAENTPLHEAATAGN+DAFKLLVEFRKEDLL LN RGET
Sbjct: 61  EQVM-------EMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLALNKRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGT-PIIHEAIQSENFEMVMMLLDFD 196
           PLYRAARYGQLQ+VEYILNECEDYY+RSP NWMA GT PIIHEAIQSENFEMVMML+DFD
Sbjct: 121 PLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFEMVMMLVDFD 180

Query: 197 KSLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           KSLLEM+DSK +TALHVLANMPHIFESGYVKGFW ELIYN
Sbjct: 181 KSLLEMKDSKNQTALHVLANMPHIFESGYVKGFWGELIYN 213

[Close]

BLAST of Cp4.1LG02g09550 vs. NCBI nr
Match: XP_023523820.1 (uncharacterized protein LOC111787944 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374 bits (961), Expect = 4.12e-123
Identity = 184/220 (83.64%), Postives = 204/220 (92.73%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M++Y+E+KQL+KLALRGEW++L +VCK ARQL++PINNSGETALHLAV+SGKEEPLKTIL
Sbjct: 1   MDLYDERKQLHKLALRGEWKNLDKVCKGARQLVLPINNSGETALHLAVYSGKEEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQ++       E +YTEHWKSSAENTPLHEAATAGN+DAFKLLVEFRKEDLLELNSRGET
Sbjct: 61  EQVM-------EENYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLELNSRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGT-PIIHEAIQSENFEMVMMLLDFD 196
           PLYRAA+YGQLQ+VEYILNECEDYYSRSPRNWM  GT PIIHEAIQSENFE+ MMLLDFD
Sbjct: 121 PLYRAAKYGQLQIVEYILNECEDYYSRSPRNWMGDGTTPIIHEAIQSENFEVAMMLLDFD 180

Query: 197 KSLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           KSLLEMRDS K+TALH+LANMPHIFESGYVKGFW ELIYN
Sbjct: 181 KSLLEMRDSNKQTALHLLANMPHIFESGYVKGFWGELIYN 213

[Close]

BLAST of Cp4.1LG02g09550 vs. NCBI nr
Match: XP_022954412.1 (espin-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 339 bits (869), Expect = 5.05e-115
Identity = 162/222 (72.97%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M++ ++ K+ Y LA+RGEW +L +VCK+A +L++PI  SG+T+LHLAVHSGKEEPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
            Q++       E++YT +WKS AENTPLHEAAT GN+ A KLLVE RKEDLLE+N RGET
Sbjct: 61  AQVM-------EIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYG+L++VEYILNECEDYY+RSP NWMAKGTPIIH AIQSENFEMVMML+DFDK
Sbjct: 121 PLYRAARYGKLEIVEYILNECEDYYTRSPLNWMAKGTPIIHAAIQSENFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYNCNN 238
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW +LIY+CNN
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWAKLIYDCNN 215

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL
Match: A0A6J1GSX9 (espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456674 PE=4 SV=1)

HSP 1 Score: 382 bits (982), Expect = 8.98e-133
Identity = 187/219 (85.39%), Postives = 200/219 (91.32%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           MNVYEEKKQLYKLALRGEWRHLREVCKDA QL++PINNSGETALHLAV+SGK EPLKTIL
Sbjct: 1   MNVYEEKKQLYKLALRGEWRHLREVCKDASQLVLPINNSGETALHLAVYSGKGEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQ++       EMDY E+WKS  ENTPLHEAAT GN+DAFKLLVEFRKEDLLELN RGET
Sbjct: 61  EQVM-------EMDYIEYWKSYVENTPLHEAATVGNLDAFKLLVEFRKEDLLELNKRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYGQLQ+VEYILNECEDYYSRSP NWM  GTPIIHEAIQSE+FEMVMML+DFDK
Sbjct: 121 PLYRAARYGQLQIVEYILNECEDYYSRSPLNWMVDGTPIIHEAIQSESFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW ELIYN
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWGELIYN 212

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL
Match: A0A6J1GSG9 (espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456725 PE=4 SV=1)

HSP 1 Score: 367 bits (942), Expect = 2.88e-126
Identity = 182/220 (82.73%), Postives = 197/220 (89.55%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M +YEEKKQLYKL LRGEWR L EVCK ARQL+ PI ++ +TA HLAV+SGK+EPLKTIL
Sbjct: 1   MGLYEEKKQLYKLVLRGEWRRLLEVCKGARQLVFPITSNRDTAFHLAVYSGKKEPLKTIL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
           EQ++       EMDYTEHWKSSAENTPLHEAATAGN+DAFKLLVEFRKEDLL LN RGET
Sbjct: 61  EQVM-------EMDYTEHWKSSAENTPLHEAATAGNLDAFKLLVEFRKEDLLALNKRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGT-PIIHEAIQSENFEMVMMLLDFD 196
           PLYRAARYGQLQ+VEYILNECEDYY+RSP NWMA GT PIIHEAIQSENFEMVMML+DFD
Sbjct: 121 PLYRAARYGQLQIVEYILNECEDYYTRSPLNWMADGTTPIIHEAIQSENFEMVMMLVDFD 180

Query: 197 KSLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           KSLLEM+DSK +TALHVLANMPHIFESGYVKGFW ELIYN
Sbjct: 181 KSLLEMKDSKNQTALHVLANMPHIFESGYVKGFWGELIYN 213

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL
Match: A0A6J1GQX5 (espin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1)

HSP 1 Score: 339 bits (869), Expect = 2.44e-115
Identity = 162/222 (72.97%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M++ ++ K+ Y LA+RGEW +L +VCK+A +L++PI  SG+T+LHLAVHSGKEEPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
            Q++       E++YT +WKS AENTPLHEAAT GN+ A KLLVE RKEDLLE+N RGET
Sbjct: 61  AQVM-------EIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYG+L++VEYILNECEDYY+RSP NWMAKGTPIIH AIQSENFEMVMML+DFDK
Sbjct: 121 PLYRAARYGKLEIVEYILNECEDYYTRSPLNWMAKGTPIIHAAIQSENFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYNCNN 238
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW +LIY+CNN
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWAKLIYDCNN 215

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL
Match: A0A6J1GR15 (espin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1)

HSP 1 Score: 331 bits (848), Expect = 3.58e-112
Identity = 159/219 (72.60%), Postives = 190/219 (86.76%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M++ ++ K+ Y LA+RGEW +L +VCK+A +L++PI  SG+T+LHLAVHSGKEEPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
            Q++       E++YT +WKS AENTPLHEAAT GN+ A KLLVE RKEDLLE+N RGET
Sbjct: 61  AQVM-------EIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYG+L++VEYILNECEDYY+RSP NWMAKGTPIIH AIQSENFEMVMML+DFDK
Sbjct: 121 PLYRAARYGKLEIVEYILNECEDYYTRSPLNWMAKGTPIIHAAIQSENFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYN 235
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW +LIY+
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWAKLIYD 212

[Close]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL
Match: A0A6J1GQZ1 (ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC111456726 PE=4 SV=1)

HSP 1 Score: 339 bits (869), Expect = 1.08e-110
Identity = 162/222 (72.97%), Postives = 193/222 (86.94%), Query Frame = 0


Query: 17  MNVYEEKKQLYKLALRGEWRHLREVCKDARQLMMPINNSGETALHLAVHSGKEEPLKTIL 76
           M++ ++ K+ Y LA+RGEW +L +VCK+A +L++PI  SG+T+LHLAVHSGKEEPLKT L
Sbjct: 1   MDLSKQNKECYILAMRGEWDNLGKVCKEAWKLVLPITGSGDTSLHLAVHSGKEEPLKTFL 60

Query: 77  EQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 136
            Q++       E++YT +WKS AENTPLHEAAT GN+ A KLLVE RKEDLLE+N RGET
Sbjct: 61  AQVM-------EIEYTLYWKSFAENTPLHEAATVGNLGAVKLLVELRKEDLLEMNIRGET 120

Query: 137 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 196
           PLYRAARYG+L++VEYILNECEDYY+RSP NWMAKGTPIIH AIQSENFEMVMML+DFDK
Sbjct: 121 PLYRAARYGKLEIVEYILNECEDYYTRSPLNWMAKGTPIIHAAIQSENFEMVMMLVDFDK 180

Query: 197 SLLEMRDSKKRTALHVLANMPHIFESGYVKGFWVELIYNCNN 238
           SLLEM+DSK +TALHVLANMPHIFESGY+KGFW +LIY+CNN
Sbjct: 181 SLLEMKDSKNQTALHVLANMPHIFESGYLKGFWAKLIYDCNN 215

[Close]

BLAST of Cp4.1LG02g09550 vs. TAIR 10
Match: AT5G60070.1 (ankyrin repeat family protein )

HSP 1 Score: 73.2 bits (178), Expect = 3.2e-13
Identity = 62/224 (27.68%), Postives = 103/224 (45.98%), Query Frame = 0


Query: 22  EKKQLYKLALRGEWRHLREVCK-------DARQLMMPINNSGETALHLAVHSGKEEPLKT 81
           E  QL     RG++  ++E+         + R L+   N  GETAL++A   G  + +  
Sbjct: 32  EDSQLLSAVRRGDFSAVKEILSNHMESEDELRDLLRKQNQCGETALYVAAEYGDADVVAE 91

Query: 82  ILEQLVLS---------------INNSGEMD-----YTEHWKSS-----AENTPLHEAAT 141
           +++   L                    GE+D       EH + S     +  T LH AA 
Sbjct: 92  LIKYYDLEDAETKARNGFDPFHIAAKQGELDVLRVLMEEHPELSMTVDLSNTTALHTAAA 151

Query: 142 AGNMDAFKLLVEFRKEDLLEL-NSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRNW 201
            G+++  + L+E     L  +  S G+T L+ AAR G  +VV+ I+    D  +R+ +  
Sbjct: 152 QGHVEVVEYLLEAAGSSLAAIAKSNGKTALHSAARNGHAEVVKAIVAVEPDTATRTDK-- 211

Query: 202 MAKGTPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHV 213
             KG   +H A++ ++ ++V+ L+   +S L M DSK  TALHV
Sbjct: 212 --KGQTPLHMAVKGQSIDVVVELMKGHRSSLNMADSKGNTALHV 251

[Close]

BLAST of Cp4.1LG02g09550 vs. TAIR 10
Match: AT1G07710.1 (Ankyrin repeat family protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.8e-11
Identity = 54/194 (27.84%), Postives = 90/194 (46.39%), Query Frame = 0


Query: 26  LYKLALRGEWRHLREV--CKDARQLMMPINNSGETALHLAVHSGKEEPLKTILE---QLV 85
           LY  A  G+   ++E+  C D   + +   N G  A H+A   G  + LK + E   +L 
Sbjct: 70  LYVAAEYGDVEIVKEMINCYDLALVEIKARN-GFDAFHIAAKQGDLDVLKVLAEAHSELA 129

Query: 86  LSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGETPLYR 145
           ++++ S               T LH AAT G+ +    L+E          S G+T L+ 
Sbjct: 130 MTVDLS-------------NTTALHTAATQGHTEVVNFLLELGSSLAGIAKSNGKTALHS 189

Query: 146 AARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDKSLLE 205
           A+R G ++V++ +L        R  +    KG   +H A++  N E+V  L+  D+S + 
Sbjct: 190 ASRNGHVKVIKALLASEPAIAIRMDK----KGQTALHMAVKGTNVEVVEELIKADRSSIN 245

Query: 206 MRDSKKRTALHVLA 215
           + D+K  TALH+ A
Sbjct: 250 IADTKGNTALHIAA 245

[Close]

BLAST of Cp4.1LG02g09550 vs. TAIR 10
Match: AT5G02620.1 (ankyrin-like1 )

HSP 1 Score: 66.6 bits (161), Expect = 3.0e-11
Identity = 50/197 (25.38%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 44  DARQLMMPINNSGETALHLAVHSGKEEPLKTI---------------------------- 103
           + ++L+   N SGETAL++A   G  + +K +                            
Sbjct: 44  ELKELLAEQNQSGETALYVAAEYGYTDMVKILMKHSDSVLAGTKAKNGFDAFHIAAKNGN 103

Query: 104 LEQLVLSINNSGEMDYTEHWKSSAENTPLHEAATAGNMDAFKLLVEFRKEDLLELNSRGE 163
           L+ L + I  + E+ +T     S++ T LH AA+ G+ +    L++   +      S G+
Sbjct: 104 LQVLDVLIEANPELSFT---FDSSKTTALHTAASQGHGEIVCFLLDKGVDLAAIARSNGK 163

Query: 164 TPLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFD 213
           T L+ AAR G   +V+ ++ +     +R  +    KG   +H A++ +N E+V +L++ D
Sbjct: 164 TALHSAARNGHTVIVKKLIEKKAGMVTRVDK----KGQTALHMAVKGQNTEIVDVLMEAD 223

[Close]

BLAST of Cp4.1LG02g09550 vs. TAIR 10
Match: AT1G03670.1 (ankyrin repeat family protein )

HSP 1 Score: 65.5 bits (158), Expect = 6.7e-11
Identity = 50/169 (29.59%), Postives = 79/169 (46.75%), Query Frame = 0


Query: 48  LMMPINNSGETALHLAVHSGKEEPLKTILEQLVLSINNSGEMDYTEHWKSSAENTPLHEA 107
           L+  +N  GET LH+A  +G       I+E LV  I  S   D     KS   +T LH A
Sbjct: 98  LLQNVNLMGETTLHVAARAGS----LNIVEILVRFITESSSYDAFIAAKSKNGDTALHAA 157

Query: 108 ATAGNMDAFKLLVEFRKEDLLELNSRGETPLYRAARYGQLQVVEYILNECEDYYSRSPRN 167
               +++    LV  + +   + N+   +PLY A   G     E +L   E   S S   
Sbjct: 158 LKGKHVEVAFCLVSVKHDVSFDKNNDEASPLYMAVEAG---YHELVLKMLESSSSPSILA 217

Query: 168 WMAKGTPIIHEAIQSENFEMVMMLLDFDKSLLEMRDSKKRTALHVLANM 217
            M  G  +IH A+++   +++ ++L  D  L+E+R+ + RT L   A+M
Sbjct: 218 SMFSGKSVIHAAMKANRRDILGIVLRQDPGLIELRNEEGRTCLSYGASM 259

[Close]

BLAST of Cp4.1LG02g09550 vs. TAIR 10
Match: AT2G01680.1 (Ankyrin repeat family protein )

HSP 1 Score: 59.7 bits (143), Expect = 3.7e-09
Identity = 45/196 (22.96%), Postives = 86/196 (43.88%), Query Frame = 0


Query: 42  CKDARQLMMPINNSGETALHLAVHSGKEEPLKTILEQLVL---SINNSGEM--------- 101
           C    +LM   N++GETA++++     E+  + ++    L    I +  +M         
Sbjct: 45  CSAVAELMSVQNDAGETAVYISAAENLEDIFRYLIRFSSLETVKIRSKSDMNAFHVAAKR 104

Query: 102 -------DYTEHWKS-----SAENT-PLHEAATAGNMDAFKLLVEFRKEDLLELNSRGET 161
                  +    W        A NT PL+ AA   +++    +++      + +   G+T
Sbjct: 105 GHLGIVKELLRLWPELCRICDASNTSPLYAAAVQDHLEIVNAMLDVDPSCAMIVRKNGKT 164

Query: 162 PLYRAARYGQLQVVEYILNECEDYYSRSPRNWMAKGTPIIHEAIQSENFEMVMMLLDFDK 213
            L+ A RYG L++V+ ++ +         +    KG   +H A++  + E+V  +L  D 
Sbjct: 165 SLHTAGRYGLLRIVKALIEKDAAIVGVKDK----KGQTALHMAVKGRSLEVVEEILQADY 224

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g09550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B1AK53	1.3e-11	30.12	Espin OS=Homo sapiens OX=9606 GN=ESPN PE=1 SV=1	[more]
Q9ET47	2.3e-11	30.12	Espin OS=Mus musculus OX=10090 GN=Espn PE=1 SV=2	[more]
Q63618	2.5e-10	30.12	Espin OS=Rattus norvegicus OX=10116 GN=Espn PE=1 SV=2	[more]
Q6AWW5	4.3e-10	25.38	Ankyrin repeat-containing protein At5g02620 OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q92625	2.8e-09	30.30	Ankyrin repeat and SAM domain-containing protein 1A OS=Homo sapiens OX=9606 GN=A...	[more]

BLAST of Cp4.1LG02g09550 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012617.1	1.24e-138	91.82	Espin, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022954414.1	1.86e-132	85.39	espin-like [Cucurbita moschata]	[more]
XP_022954460.1	5.95e-126	82.73	espin-like [Cucurbita moschata]	[more]
XP_023523820.1	4.12e-123	83.64	uncharacterized protein LOC111787944 [Cucurbita pepo subsp. pepo]	[more]
XP_022954412.1	5.05e-115	72.97	espin-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG02g09550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSX9	8.98e-133	85.39	espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456674 PE=4 SV=1	[more]
A0A6J1GSG9	2.88e-126	82.73	espin-like OS=Cucurbita moschata OX=3662 GN=LOC111456725 PE=4 SV=1	[more]
A0A6J1GQX5	2.44e-115	72.97	espin-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1	[more]
A0A6J1GR15	3.58e-112	72.60	espin-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456673 PE=4 SV=1	[more]
A0A6J1GQZ1	1.08e-110	72.97	ankyrin repeat-containing protein ITN1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]

BLAST of Cp4.1LG02g09550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60070.1	3.2e-13	27.68	ankyrin repeat family protein	[more]
AT1G07710.1	1.8e-11	27.84	Ankyrin repeat family protein	[more]
AT5G02620.1	3.0e-11	25.38	ankyrin-like1	[more]
AT1G03670.1	6.7e-11	29.59	ankyrin repeat family protein	[more]
AT2G01680.1	3.7e-09	22.96	Ankyrin repeat family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002110	Ankyrin repeat	SMART	SM00248	ANK_2a	coord: 99..129 e-value: 0.97 score: 18.5 coord: 171..200 e-value: 170.0 score: 10.7 coord: 133..162 e-value: 0.13 score: 21.3 coord: 55..85 e-value: 0.28 score: 20.3
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 133..165 score: 9.61777
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 99..121 score: 8.60279
IPR002110	Ankyrin repeat	PROSITE	PS50088	ANK_REPEAT	coord: 55..77 score: 8.70963
IPR020683	Ankyrin repeat-containing domain	PFAM	PF12796	Ank_2	coord: 77..156 e-value: 8.2E-11 score: 42.4
IPR036770	Ankyrin repeat-containing domain superfamily	GENE3D	1.25.40.20		coord: 22..221 e-value: 2.7E-31 score: 110.6
IPR036770	Ankyrin repeat-containing domain superfamily	SUPERFAMILY	48403	Ankyrin repeat	coord: 28..216
None	No IPR available	PANTHER	PTHR24177	CASKIN	coord: 37..232
None	No IPR available	PANTHER	PTHR24177:SF331	REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATED	coord: 37..232
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 55..77 score: 8.693567
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 99..121 score: 8.58744
None	No IPR available	PROSITE	PS50297	ANK_REP_REGION	coord: 133..155 score: 9.33032

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g09550.1	Cp4.1LG02g09550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005515	protein binding