Cp4.1LG02g09030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g09030
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein kinase 2B
LocationCp4.1LG02: 6124841 .. 6128862 (-)
RNA-Seq ExpressionCp4.1LG02g09030
SyntenyCp4.1LG02g09030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAGTTCTTGCAGACTTCACCTTCCATTTTTGTTACCCAATTCCTCTGCCCCTTTCAGCTTTCGATGGGTAACTGCTTTGGTTCTTCTGCCAAAGTTGAAATCGCCCACAGCTCCCGACCCACTTCCGGTAACACTTCTCTCCATCTCTTTCTCTATCAATTTTAGTTTCTGTTCTCGTAATTTCTGTGTTTTTGATCTTCCCTGTGCTTATGGTTTCTGGGTTTTCATTCGATTTTTCATTGATTCGATGTTGTGTTTGGTTTAGTTCTTGGGAATTTTTGGTAGATTCTGCTGTTTGTTCCTTGAAGTTTCAAATTCTTTATTCTGTTTCGAAAGAAACGAGGATTTTAAGTTTTGTTTTCGTTTATGGATCAGAAATCTCCCAAAGTTCAAGCAAGAAACATAATCCGATTGTTCCTTCCTATAATCCTAACTCAGTGGTGGATAGGAATGATAATAATAATAATGTGTTGAATCTTCCCACACCAAGAACAGAAGGTGAAATACTGTCATCTTCGAATTTGAAGCCCTTCCATTTCAATGAGCTGAAGAACGCGACCAGAAATTTTCGACCCGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGGTGCTGCGAGGCCTGGATTGGGAATGGTTGTGGCTGTCAAGAAGCTTAAGGCTGAAGCTTCCCAAGGCCACAAGGAGTGGCTGGTGCGTAGTGTAGTTGATGTTTTGTCATGTGTTCCTTTTATGTTATAACATAAGCTTGTGTCTGCTACTAACTGCTTATAAATTCAGACAGAAGTTGATTATTTGGGCAAATTTCATCATCCAAATCTTGTTAAGCTGATTGGGTATTGCTTGGAAGGCGAAAATCGGCTACTGGTTTACGAGTTTATGCCCAGAGGGAGCTTGGAGAATCATCTCTTCAGAAGTAAGTTTTGTATCCATTTAGATGTTGAAAGTGACTCTGTTTCTTAAACATTCTTCCATGAAATTTGTTTGCCTCCCTCTCTCAAATGCTACAATTATCAGTTTTGTATCCATTTTGTATCCATTTATGTGTGTTTTCCGGTTTGAACTGGATTACGAGTACTCAATGCGTTCAATGTTAGGAATCACGAACCTCCACAATGGTATGATCCACTTATAAACCCATGATCAACCCCTTCATTAGTCGATGTGGGACTCCTCTCCCAACAATTCTCAACAATCCTCCCTTGAACAAAGTATACCATAGAGCCTCCTATGGAACCCTCGAATAGCCTCTCCTTAATCGAGGCTCGACTCCTTTTCTGGAGCACTCGAACAAAGTACACCCTTTTGTTCGATATTTGAGTCACTTTTAACTACACCTTTGAGCCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGGTTAAGTTAAAGGCATGACTCGGATACCATTTTAGGAATCACGAACCTCCACAATGGTATGATATTGTCCCCTTTGAGTATAAGCTCTCATAGCTTTACTTTTAGTTTTCCCAAAAGGGGTGTATTTCTTTATAAACCTATGATCAACCCCTTAATTAGTCGACATGGGACTCCTCTCCCAACAATTCTCAACAATCCTCCCTCGAGCAAAGTATACCATAGAGCCTCCTCTGAGGCCTATGGAACCCTCGAATAGCCTCTCCTTAATCGAGGCTCTACTCCTTCTCTGGAGCCCTTGAACAAAGTACACCATTTGTTCGATATTTGAGTCACTTAACTACACCTTTGAGCCTCACAACTTCTTTGTTCGACATTTGAGGATTCTATTGACATGACTAAGTTAAGGGCATGACTCGGATATCATTTTAGGAATCACGAACCTCTACAATGGTATGATATTGTCCACTTTGAACATAAGCTCTCGTAGTGAGAAAAGTGTTTTTACATGAGTTTGGATTTGATATGGGGGCCTAGTTGATTTGCCAAGTTCTGTTGTGCAGGGGGTTCCCAACCTCTTTCTTGGGCATTAAGGATCAAAGTGGCCATAGCTGCAGCCAGAGGGCTTTCCTTTCTTCACGAAGCCGAATCGCCCGTCATATATCGCGATTTCAAGGCATCGAACATCCTACTCGACGCAGTAAGAAAGCCGTTTCTGTTCTGCACAAATGATATACAATGTTGTTACAATTGTTGTTCATAATTTTGTGCATCCTTAATGCAGGAATTTAATGCAAAGCTTTCTGATTTTGGTTTGGCTAAGGCTGGTCCCACTGGAGATAGAACTCATGTTACTACTCAAGTTATGGGAACCCGTGGCTACGCTGCACCCGAATATATCGCTACAGGTTCGTAACGCTTGCCCTATACTCCAGTTCTGCACAATGTTGATGATTTCATCTTTTATTTACATAACATGATATGCTTATCTTATCTCCTAATCTCTGCATCATTCTACTACCCCAATCATGATCTTTGGAAGCTTTTGGTATGATCTAGAATCTGTAGTGACCTAAAATGAACATTAGGATTCGAACGTAAATACACCATTCTAGGTCACTGCCACACATGGTCTAGCTAGGGGTATGCACGGTACAGTTGGGTTGGAGAAGTTTTTTGGACCAATTCGAAATTTGGGTTGTTTTGGTTGATGGTGATTCAAATGATCTGAACGGAGTTCATGACCCAACTCTCCAATTTCGGGTTGGGTTGGGTTGGATTGGATTGTGAACCCTATTTCGATTTGGTTGGGTTGACTAGACCAAAAGAGTTTCACCCCATGAATTGAATTGAATTTTTTGATTTTCTAAAAACTCAACCCAACACAGGGTCAAGTTGTTCGAGTAAATGAATCATACGAACACCTCTAGTTTCAGATCCTAAGTTCCATCCAAGATTATAATGAATTTTTACTTCAAGGTAGGTTTAAAACATTTACGAAAGCTCAAATGTAATATTACAACTTTTGAAATAATGTGTAAAGTTAGAGATGTTTAACCTATCAAGAGAAGACTCATTGGTATTTTGTATTCAAGGCGTTTCTATGAACGCCGATTTCATTAGGTCATTTTATTAACATTGTTTGCACTCTCAACAATCCGTTATTCGACTAAACAAAAGACTCATCAACTTTTTTTTTGTTCGATACTCACGGATTCCAACCCACACAACTAATTAAGTTAGGGATATCACTTTGATACCACTTATAAGAGACAACGACGCTTGTATCTCTATAATGATATTATATTGTCCCCTTTGAGCTTAAGCCAAACTTTTCAAATCTATGATAATTAACGGTTTAAATTGCAACGTCGTTTACTCCTCAGGTCGATTGACATCTAAAAGCGATGTTTACAGTTTTGGAGTTGTGTTGCTCGAATTGTTATCGGGTTTATGTGCTGAAGACAATCTTGTAGAGTGGGCAACACCATATTTAGGTGATAAAAGAAGGTTATTTCGAATTATGGACACCAAACTTGAAGGACGATATTCTAAAAAGGGAGCTTATGTAGCTGCAAATTTAGCTTCACAATGTCTTAGGAGAGAACCGAGGGCCCGACCTCGAATGACAGAGATTTTGGGTGAATTGGAGGAGCTTGAATCCCCAAAAACTACCGCTAGAATCTCACACTCAGTTCAACGTGAAACTCCTGCTTTGTCAAACTCGAAGGTTGATGCATCTCTTTCGACATTGTATCAAAAGTCACCACGACGACAATAACATCGTTAAATTAGTTGCATATAAATTGAGAGGTGGAGCCGAACATATTCAAAACAAAAGATGGTAAACCTATAACTAAAGCGTGACCTTGTATATGTTCTTCTTTAGAAAACGATTATCGATGACTATTGTTTGTGATTTATTAGACATGAATGTAGGTCTTATCCTATTAAGAACGCCCTGACAAATTTGATCGTTTTAGTTGAGTGAGAATTCATGAAATTAATTAGCTTTTCGAGAAATTATTTTTTGGCAATATGATAATGTAACGGTAGTAATAGAAAAGTACCATTACACCCTTTTAATCTTTATATATATATAT

mRNA sequence

TATAGTTCTTGCAGACTTCACCTTCCATTTTTGTTACCCAATTCCTCTGCCCCTTTCAGCTTTCGATGGGTAACTGCTTTGGTTCTTCTGCCAAAGTTGAAATCGCCCACAGCTCCCGACCCACTTCCGATTCTGCTGTTTGTTCCTTGAAGTTTCAAATTCTTTATTCTGTTTCGAAAGAAACGAGGATTTTAAGTTTTGTTTTCGTTTATGGATCAGAAATCTCCCAAAGTTCAAGCAAGAAACATAATCCGATTGTTCCTTCCTATAATCCTAACTCAGTGGTGGATAGGAATGATAATAATAATAATGTGTTGAATCTTCCCACACCAAGAACAGAAGGTGAAATACTGTCATCTTCGAATTTGAAGCCCTTCCATTTCAATGAGCTGAAGAACGCGACCAGAAATTTTCGACCCGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGGTGCTGCGAGGCCTGGATTGGGAATGGTTGTGGCTGTCAAGAAGCTTAAGGCTGAAGCTTCCCAAGGCCACAAGGAGTGGCTGGTGCGTAGTGTAGTTGATGTTTTGTCATGTGTTCCTTTTATGGGGTTCCCAACCTCTTTCTTGGGCATTAAGGATCAAAGTGGCCATAGCTGCAGCCAGAGGGCTTTCCTTTCTTCACGAAGCCGAATCGCCCGTCATATATCGCGATTTCAAGGCATCGAACATCCTACTCGACGCAGTCGATTGACATCTAAAAGCGATGTTTACAGTTTTGGAGTTGTGTTGCTCGAATTGTTATCGGGTTTATGTGCTGAAGACAATCTTGTAGAGTGGGCAACACCATATTTAGGTGATAAAAGAAGGTTATTTCGAATTATGGACACCAAACTTGAAGGACGATATTCTAAAAAGGGAGCTTATGTAGCTGCAAATTTAGCTTCACAATGTCTTAGGAGAGAACCGAGGGCCCGACCTCGAATGACAGAGATTTTGGGTGAATTGGAGGAGCTTGAATCCCCAAAAACTACCGCTAGAATCTCACACTCAGTTCAACGTGAAACTCCTGCTTTGTCAAACTCGAAGGTTGATGCATCTCTTTCGACATTGTATCAAAAGTCACCACGACGACAATAACATCGTTAAATTAGTTGCATATAAATTGAGAGGTGGAGCCGAACATATTCAAAACAAAAGATGGTAAACCTATAACTAAAGCGTGACCTTGTATATGTTCTTCTTTAGAAAACGATTATCGATGACTATTGTTTGTGATTTATTAGACATGAATGTAGGTCTTATCCTATTAAGAACGCCCTGACAAATTTGATCGTTTTAGTTGAGTGAGAATTCATGAAATTAATTAGCTTTTCGAGAAATTATTTTTTGGCAATATGATAATGTAACGGTAGTAATAGAAAAGTACCATTACACCCTTTTAATCTTTATATATATATAT

Coding sequence (CDS)

ATGGGTAACTGCTTTGGTTCTTCTGCCAAAGTTGAAATCGCCCACAGCTCCCGACCCACTTCCGATTCTGCTGTTTGTTCCTTGAAGTTTCAAATTCTTTATTCTGTTTCGAAAGAAACGAGGATTTTAAGTTTTGTTTTCGTTTATGGATCAGAAATCTCCCAAAGTTCAAGCAAGAAACATAATCCGATTGTTCCTTCCTATAATCCTAACTCAGTGGTGGATAGGAATGATAATAATAATAATGTGTTGAATCTTCCCACACCAAGAACAGAAGGTGAAATACTGTCATCTTCGAATTTGAAGCCCTTCCATTTCAATGAGCTGAAGAACGCGACCAGAAATTTTCGACCCGACAGCCTGCTAGGTGAAGGGGGATTTGGTTATGTTTTCAAAGGATGGATTGATGAAAACACATTGGGTGCTGCGAGGCCTGGATTGGGAATGGTTGTGGCTGTCAAGAAGCTTAAGGCTGAAGCTTCCCAAGGCCACAAGGAGTGGCTGGTGCGTAGTGTAGTTGATGTTTTGTCATGTGTTCCTTTTATGGGGTTCCCAACCTCTTTCTTGGGCATTAAGGATCAAAGTGGCCATAGCTGCAGCCAGAGGGCTTTCCTTTCTTCACGAAGCCGAATCGCCCGTCATATATCGCGATTTCAAGGCATCGAACATCCTACTCGACGCAGTCGATTGACATCTAAAAGCGATGTTTACAGTTTTGGAGTTGTGTTGCTCGAATTGTTATCGGGTTTATGTGCTGAAGACAATCTTGTAGAGTGGGCAACACCATATTTAGGTGATAAAAGAAGGTTATTTCGAATTATGGACACCAAACTTGAAGGACGATATTCTAAAAAGGGAGCTTATGTAGCTGCAAATTTAGCTTCACAATGTCTTAGGAGAGAACCGAGGGCCCGACCTCGAATGACAGAGATTTTGGGTGAATTGGAGGAGCTTGAATCCCCAAAAACTACCGCTAGAATCTCACACTCAGTTCAACGTGAAACTCCTGCTTTGTCAAACTCGAAGGTTGATGCATCTCTTTCGACATTGTATCAAAAGTCACCACGACGACAATAA

Protein sequence

MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKKHNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVLSCVPFMGFPTSFLGIKDQSGHSCSQRAFLSSRSRIARHISRFQGIEHPTRRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKKGAYVAANLASQCLRREPRARPRMTEILGELEELESPKTTARISHSVQRETPALSNSKVDASLSTLYQKSPRRQ
Homology
BLAST of Cp4.1LG02g09030 vs. ExPASy Swiss-Prot
Match: O49840 (Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN=PBL3 PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.5e-64
Identity = 176/439 (40.09%), Postives = 223/439 (50.80%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ + S  P ++SA  S                       S +S  +S+ 
Sbjct: 1   MGNCLDSSAKVD-SSSHSPHANSASLS-----------------------SRVSSKTSRS 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
             P   S N  S V+         +LPTPRTEGEILSS NLK F FNELKNATRNFRPDS
Sbjct: 61  TVPSSLSINSYSSVE---------SLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL------------ 180
           LLGEGGFGYVFKGWID  TL A++PG G+VVAVKKLK E  QGHKEWL            
Sbjct: 121 LLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHP 180

Query: 181 ------------------------------------------VRSVV------------D 240
                                                     +R  V            D
Sbjct: 181 NLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHD 240

Query: 241 VLSCVPFMGFPTSFLGIKDQ-----SGHSCSQRAFLSSRSRIARHISRFQGIEHP--TRR 300
             S V +  F  + + +  +     S    ++      ++ ++  +    G   P     
Sbjct: 241 AKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCA--------EDNLVEWATPYLGDKRRLFRIMDTKLE 358
            RLT+KSDVYSFGVVLLELLSG  A        E +LV+WATPYLGDKR+LFRIMDT+L 
Sbjct: 301 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 360

BLAST of Cp4.1LG02g09030 vs. ExPASy Swiss-Prot
Match: Q5PP29 (Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN=PBL4 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 2.0e-59
Identity = 165/423 (39.01%), Postives = 211/423 (49.88%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFG SAKV   +   P   S+  S K       S+ +R+ S          QSS   
Sbjct: 1   MGNCFGFSAKV--GNRESPYRGSSRISAK------RSQSSRLSSLTI-------QSS--- 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
                 SYN         ++ +V +L TPR+EGE+L+S  LK F FNELK ATRNFRPDS
Sbjct: 61  ------SYN---------DDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVLSCVP 180
           ++GEGGFGYV+KGWIDE TL  ++PG GMVVAVKKLK E  QGH++WL    VD L  + 
Sbjct: 121 VIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAE--VDCLGRLH 180

Query: 181 FMGFPTSFLGIKDQSGH-------------------------------------SCSQRA 240
            M      +G   +  H                                     +    A
Sbjct: 181 HMNL-VKLIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLA 240

Query: 241 FL------------------------------------SSRSRIARHISRFQGIEHP--T 300
           FL                                      R+ ++  +   QG   P   
Sbjct: 241 FLHEAQVIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYV 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSG--------LCAEDNLVEWATPYLGDKRRLFRIMDTK 341
              R+T+KSDVYSFGVVLLELLSG        +  E NLV+WA PYLGDKR++FRIMDTK
Sbjct: 301 ATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTK 360

BLAST of Cp4.1LG02g09030 vs. ExPASy Swiss-Prot
Match: O49839 (Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN=PBL2 PE=1 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 1.2e-56
Identity = 167/438 (38.13%), Postives = 214/438 (48.86%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ ++ S P ++SA                         GS++S  +S+ 
Sbjct: 1   MGNCLDSSAKVDNSNHS-PHANSASS-----------------------GSKVSSKTSRS 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
             P   S    S       +++   LPT RTEGEILSS NLK F FNELKNAT+NFR D+
Sbjct: 61  TGPSGLSTTSYS------TDSSFGPLPTLRTEGEILSSPNLKAFTFNELKNATKNFRQDN 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL------------ 180
           LLGEGGFG VFKGWID+ +L A+RPG G+VVAVK+LK E  QGHKEWL            
Sbjct: 121 LLGEGGFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHP 180

Query: 181 --------------------------------------------VRSVVDVLSCVPFMGF 240
                                                       ++  V     + F+  
Sbjct: 181 NLVLLVGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHE 240

Query: 241 PTSFLGIKD---------------QSGHSCSQRAFLSSRSRIARHISRFQGIEHP--TRR 300
             S +  +D                S    ++       + ++  +    G   P     
Sbjct: 241 AKSQVIYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCAEDN--------LVEWATPYLGDKRRLFRIMDTKLE 358
            RLT+KSDVYSFGVVLLEL+SG  A DN        LV+WATPYLGDKR+LFRIMDTKL 
Sbjct: 301 GRLTAKSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLG 360

BLAST of Cp4.1LG02g09030 vs. ExPASy Swiss-Prot
Match: Q5XF79 (Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 GN=PBL18 PE=2 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 3.4e-51
Identity = 148/399 (37.09%), Postives = 192/399 (48.12%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSA+V    S                  +    +RI        S     SS+ 
Sbjct: 1   MGNCLDSSARVGNRES------------------TFGGSSRI--------SRKPNQSSRL 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
            +  +PSY+ NS    + +N       TPR+EGE+L S  LK F FNELK ATRNF+P+S
Sbjct: 61  SSLTIPSYSNNSFTTSSWSN------LTPRSEGELLPSPTLKAFTFNELKTATRNFKPNS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVR----SVVDVL 180
           ++GEGGFG V+KGWI E +L  ++PG GMVVAVKKLK+E  QGHKEWL        +  +
Sbjct: 121 MIGEGGFGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHM 180

Query: 181 SCVPFMGF--------------PTSFL--------------------------GIKDQSG 240
           + V  +G+              P   L                          G+     
Sbjct: 181 NLVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHE 240

Query: 241 HSCSQRAFLSS-------------------------RSRIARHISRFQGIEHP--TRRSR 300
                R F +S                         R+ +   +   QG   P      R
Sbjct: 241 AKVIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGR 300

Query: 301 LTSKSDVYSFGVVLLELLSG--------LCAEDNLVEWATPYLGDKRRLFRIMDTKLEGR 321
           LTSKSDVYSFGVVLLELLSG        +  E NLV+WA PYL D+R++FRIMDTKL G+
Sbjct: 301 LTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQ 360

BLAST of Cp4.1LG02g09030 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.6e-47
Identity = 138/363 (38.02%), Postives = 181/363 (49.86%), Query Frame = 0

Query: 49  YGSEISQSSSKKHNPIVPSYNPNSVVDRNDNNNNVLNL---PTPRTEGEILSSSNLKPFH 108
           +G++IS  S     P   + +P+    +  +N++V      PTPR+E EIL ++N+K F 
Sbjct: 5   WGAKISSES-----PCRSASSPSGGTSKYASNSSVSAASVPPTPRSEDEILEAANVKAFA 64

Query: 109 FNELKNATRNFRPDSLLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHK 168
           FNEL+ ATRNFRPDS+LGEGGFG VFKGWIDE TL   +PG GMV+AVKKL  E  QGH+
Sbjct: 65  FNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTGMVIAVKKLNQEGHQGHR 124

Query: 169 EWLVR-SVVDVLS---CVPFMGF-------------------------------PTSF-L 228
           EWL   + +  LS    V  +G+                               P S+ L
Sbjct: 125 EWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLENHLFRRSTHFQPLSWNL 184

Query: 229 GIKDQSGHSCSQRAFLSS--------------------------------------RSRI 288
            +K   G +    AFL S                                      +S +
Sbjct: 185 RMKIALG-AAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLSDFGLAKDGPTGDKSHV 244

Query: 289 ARHISRFQGIEHP--TRRSRLTSKSDVYSFGVVLLELLSGLCA--------EDNLVEWAT 325
           +  +    G   P       LT+KSDVYSFGVVLLE+LSG  A        E NLVEWA 
Sbjct: 245 STRVMGTYGYAAPEYLATGHLTTKSDVYSFGVVLLEMLSGRRALDKNRPTGEHNLVEWAR 304

BLAST of Cp4.1LG02g09030 vs. NCBI nr
Match: XP_023522677.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479 bits (1232), Expect = 7.64e-166
Identity = 282/433 (65.13%), Postives = 293/433 (67.67%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEISQSSSKK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISQSSSKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 360

BLAST of Cp4.1LG02g09030 vs. NCBI nr
Match: XP_022946188.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita moschata])

HSP 1 Score: 457 bits (1177), Expect = 1.67e-157
Identity = 275/433 (63.51%), Postives = 287/433 (66.28%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEIS+SS KK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISRSSCKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSY PNSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYYPNSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAKSPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGL AEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSK 
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLHAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKN 360

BLAST of Cp4.1LG02g09030 vs. NCBI nr
Match: XP_022999699.1 (probable serine/threonine-protein kinase PBL3 [Cucurbita maxima])

HSP 1 Score: 450 bits (1158), Expect = 1.28e-154
Identity = 272/433 (62.82%), Postives = 285/433 (65.82%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEIS+SSSKK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISRSSSKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSYNPNSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYNPNSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDEN L AARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENKLAAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGL AEDNLVEWA PYLGDKRRLFRIMDTKLEGRYSKK
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLRAEDNLVEWAMPYLGDKRRLFRIMDTKLEGRYSKK 360

BLAST of Cp4.1LG02g09030 vs. NCBI nr
Match: KAG7030002.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 400 bits (1028), Expect = 3.71e-135
Identity = 237/363 (65.29%), Postives = 249/363 (68.60%), Query Frame = 0

Query: 71  NSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYV 130
           NSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYV
Sbjct: 23  NSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDSLLGEGGFGYV 82

Query: 131 FKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL------SCVPFMGF 190
           FKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL    VD L      + V  +G+
Sbjct: 83  FKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFHHPNLVKLIGY 142

Query: 191 -----------------------------PTSF-------------LGIKDQSGHSCSQR 250
                                        P S+             L    ++      R
Sbjct: 143 CLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHEAKSPVIYR 202

Query: 251 AFLSS-------------------------RSRIARHISRFQGIEHPT--RRSRLTSKSD 310
            F +S                         R+ +   +   +G   P      RLTSKSD
Sbjct: 203 DFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYIATGRLTSKSD 262

Query: 311 VYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKKGAYVAANLAS 358
           VYSFGVVLLELLSGL AEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSK GAYVAANLAS
Sbjct: 263 VYSFGVVLLELLSGLRAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKNGAYVAANLAS 322

BLAST of Cp4.1LG02g09030 vs. NCBI nr
Match: KAG6599064.1 (putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392 bits (1006), Expect = 6.78e-132
Identity = 248/433 (57.27%), Postives = 260/433 (60.05%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEIS+SSSKK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISRSSSKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSYNPNSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYNPNSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAKSPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGL AEDNLVEWATPYLGDKRR                
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLRAEDNLVEWATPYLGDKRR---------------- 360

BLAST of Cp4.1LG02g09030 vs. ExPASy TrEMBL
Match: A0A6J1G338 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=LOC111450344 PE=3 SV=1)

HSP 1 Score: 457 bits (1177), Expect = 8.09e-158
Identity = 275/433 (63.51%), Postives = 287/433 (66.28%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEIS+SS KK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISRSSCKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSY PNSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYYPNSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAKSPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGL AEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSK 
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLHAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKN 360

BLAST of Cp4.1LG02g09030 vs. ExPASy TrEMBL
Match: A0A6J1KHV0 (probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC111493974 PE=3 SV=1)

HSP 1 Score: 450 bits (1158), Expect = 6.22e-155
Identity = 272/433 (62.82%), Postives = 285/433 (65.82%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFGSSAKVEIAHSSRPT                              SEIS+SSSKK
Sbjct: 1   MGNCFGSSAKVEIAHSSRPT------------------------------SEISRSSSKK 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           HNPIVPSYNPNSV DRNDNNN VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS
Sbjct: 61  HNPIVPSYNPNSVADRNDNNN-VLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDEN L AARPGLGMVVAVKKLKAEASQGHKEWL    VD L    
Sbjct: 121 LLGEGGFGYVFKGWIDENKLAAARPGLGMVVAVKKLKAEASQGHKEWLTE--VDYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 358
              RLTSKSDVYSFGVVLLELLSGL AEDNLVEWA PYLGDKRRLFRIMDTKLEGRYSKK
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGLRAEDNLVEWAMPYLGDKRRLFRIMDTKLEGRYSKK 360

BLAST of Cp4.1LG02g09030 vs. ExPASy TrEMBL
Match: A0A5A7T4R2 (Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00870 PE=3 SV=1)

HSP 1 Score: 367 bits (941), Expect = 4.24e-122
Identity = 232/432 (53.70%), Postives = 262/432 (60.65%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ AH+S+ T                               EISQ+SSK+
Sbjct: 1   MGNCLASSAKVQNAHTSQGT------------------------------HEISQNSSKR 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           H P+VPS  PNS++DRN N   V +LPTP+TEGEILSSSNLKPF FNEL+NATRNFRPDS
Sbjct: 61  H-PLVPSNLPNSLLDRNKN---VPSLPTPKTEGEILSSSNLKPFSFNELRNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENT  AARPGLGMVVAVKKLK EASQGHKEWL    V+ L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTWAAARPGLGMVVAVKKLKPEASQGHKEWLTE--VNYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 357
              RLTSKSDVYSFGVVLLELLSG  AEDNLV+WA+PYLGDKRRLFRIMDTKLEGRYSKK
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWASPYLGDKRRLFRIMDTKLEGRYSKK 360

BLAST of Cp4.1LG02g09030 vs. ExPASy TrEMBL
Match: A0A1S3C2W0 (protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE=3 SV=1)

HSP 1 Score: 367 bits (941), Expect = 4.24e-122
Identity = 232/432 (53.70%), Postives = 262/432 (60.65%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ AH+S+ T                               EISQ+SSK+
Sbjct: 1   MGNCLASSAKVQNAHTSQGT------------------------------HEISQNSSKR 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           H P+VPS  PNS++DRN N   V +LPTP+TEGEILSSSNLKPF FNEL+NATRNFRPDS
Sbjct: 61  H-PLVPSNLPNSLLDRNKN---VPSLPTPKTEGEILSSSNLKPFSFNELRNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVL---- 180
           LLGEGGFGYVFKGWIDENT  AARPGLGMVVAVKKLK EASQGHKEWL    V+ L    
Sbjct: 121 LLGEGGFGYVFKGWIDENTWAAARPGLGMVVAVKKLKPEASQGHKEWLTE--VNYLGKFH 180

Query: 181 --SCVPFMGF-----------------------------PTSF-------------LGIK 240
             + V  +G+                             P S+             L   
Sbjct: 181 HPNLVKLIGYCLEGENRLLVYEFMPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFL 240

Query: 241 DQSGHSCSQRAFLSS-------------------------RSRIARHISRFQGIEHPT-- 300
            ++      R F +S                         R+ +   +   +G   P   
Sbjct: 241 HEAESPVIYRDFKASNILLDAEFNAKLSDFGLAKAGPTGDRTHVTTQVMGTRGYAAPEYI 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKK 357
              RLTSKSDVYSFGVVLLELLSG  AEDNLV+WA+PYLGDKRRLFRIMDTKLEGRYSKK
Sbjct: 301 ATGRLTSKSDVYSFGVVLLELLSGQRAEDNLVDWASPYLGDKRRLFRIMDTKLEGRYSKK 360

BLAST of Cp4.1LG02g09030 vs. ExPASy TrEMBL
Match: A0A0A0KE20 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404180 PE=3 SV=1)

HSP 1 Score: 360 bits (923), Expect = 2.25e-119
Identity = 226/427 (52.93%), Postives = 256/427 (59.95%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ AH+S+ T                               EISQ+SSK+
Sbjct: 1   MGNCLDSSAKVQNAHTSQGT------------------------------HEISQNSSKR 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
           H P+VPS  PNS+VDRN   NNVL+LPTP+TEGEILSSSNLKPF FNEL++ATRNFRPDS
Sbjct: 61  H-PLVPSNLPNSLVDRN---NNVLSLPTPKTEGEILSSSNLKPFSFNELRSATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVR---------- 180
           LLGEGGFGYVFKGWIDENT  A RPG+GMVVAVKKLK EASQGHKEWL            
Sbjct: 121 LLGEGGFGYVFKGWIDENTWAAVRPGMGMVVAVKKLKPEASQGHKEWLTEVNYLGKFHHP 180

Query: 181 SVVDVL------------------------------------------------------ 240
           ++V ++                                                      
Sbjct: 181 NLVKLIGYCLEGENRLLVYEFLPRGSLENHLFRRGSQPLSWALRIKVAIAAARGLSFLHE 240

Query: 241 --SCVPFMGFPTSFLGIKDQSGHSCSQRAFLSS-----RSRIARHISRFQGIEHPT--RR 300
             S V +  F  S + +  +     S    + +     R+ +   +   +G   P     
Sbjct: 241 AESPVIYRDFKASNILLDAEFNAKLSDFGLVKAGPTGDRTHVTTQVIGTRGYAAPEYIAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCAEDNLVEWATPYLGDKRRLFRIMDTKLEGRYSKKGA 354
            RLTSKSDVYSFGVVLLELLSG  AEDNLVE A+PYLGDKR+LFRIMDTKLEGRYSKKGA
Sbjct: 301 GRLTSKSDVYSFGVVLLELLSGQRAEDNLVERASPYLGDKRKLFRIMDTKLEGRYSKKGA 360

BLAST of Cp4.1LG02g09030 vs. TAIR 10
Match: AT2G02800.1 (protein kinase 2B )

HSP 1 Score: 246.9 bits (629), Expect = 2.5e-65
Identity = 176/439 (40.09%), Postives = 223/439 (50.80%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ + S  P ++SA  S                       S +S  +S+ 
Sbjct: 1   MGNCLDSSAKVD-SSSHSPHANSASLS-----------------------SRVSSKTSRS 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
             P   S N  S V+         +LPTPRTEGEILSS NLK F FNELKNATRNFRPDS
Sbjct: 61  TVPSSLSINSYSSVE---------SLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL------------ 180
           LLGEGGFGYVFKGWID  TL A++PG G+VVAVKKLK E  QGHKEWL            
Sbjct: 121 LLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHP 180

Query: 181 ------------------------------------------VRSVV------------D 240
                                                     +R  V            D
Sbjct: 181 NLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHD 240

Query: 241 VLSCVPFMGFPTSFLGIKDQ-----SGHSCSQRAFLSSRSRIARHISRFQGIEHP--TRR 300
             S V +  F  + + +  +     S    ++      ++ ++  +    G   P     
Sbjct: 241 AKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCA--------EDNLVEWATPYLGDKRRLFRIMDTKLE 358
            RLT+KSDVYSFGVVLLELLSG  A        E +LV+WATPYLGDKR+LFRIMDT+L 
Sbjct: 301 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 360

BLAST of Cp4.1LG02g09030 vs. TAIR 10
Match: AT2G02800.2 (protein kinase 2B )

HSP 1 Score: 246.9 bits (629), Expect = 2.5e-65
Identity = 176/439 (40.09%), Postives = 223/439 (50.80%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ + S  P ++SA  S                       S +S  +S+ 
Sbjct: 1   MGNCLDSSAKVD-SSSHSPHANSASLS-----------------------SRVSSKTSRS 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
             P   S N  S V+         +LPTPRTEGEILSS NLK F FNELKNATRNFRPDS
Sbjct: 61  TVPSSLSINSYSSVE---------SLPTPRTEGEILSSPNLKAFTFNELKNATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL------------ 180
           LLGEGGFGYVFKGWID  TL A++PG G+VVAVKKLK E  QGHKEWL            
Sbjct: 121 LLGEGGFGYVFKGWIDGTTLTASKPGSGIVVAVKKLKTEGYQGHKEWLTEVNYLGQLSHP 180

Query: 181 ------------------------------------------VRSVV------------D 240
                                                     +R  V            D
Sbjct: 181 NLVKLVGYCVEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAIGAAKGLTFLHD 240

Query: 241 VLSCVPFMGFPTSFLGIKDQ-----SGHSCSQRAFLSSRSRIARHISRFQGIEHP--TRR 300
             S V +  F  + + +  +     S    ++      ++ ++  +    G   P     
Sbjct: 241 AKSQVIYRDFKAANILLDAEFNSKLSDFGLAKAGPTGDKTHVSTQVMGTHGYAAPEYVAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCA--------EDNLVEWATPYLGDKRRLFRIMDTKLE 358
            RLT+KSDVYSFGVVLLELLSG  A        E +LV+WATPYLGDKR+LFRIMDT+L 
Sbjct: 301 GRLTAKSDVYSFGVVLLELLSGRRAVDKSKVGMEQSLVDWATPYLGDKRKLFRIMDTRLG 360

BLAST of Cp4.1LG02g09030 vs. TAIR 10
Match: AT1G26970.1 (Protein kinase superfamily protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.4e-60
Identity = 165/423 (39.01%), Postives = 211/423 (49.88%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNCFG SAKV   +   P   S+  S K       S+ +R+ S          QSS   
Sbjct: 1   MGNCFGFSAKV--GNRESPYRGSSRISAK------RSQSSRLSSLTI-------QSS--- 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
                 SYN         ++ +V +L TPR+EGE+L+S  LK F FNELK ATRNFRPDS
Sbjct: 61  ------SYN---------DDTSVASLQTPRSEGELLASPTLKAFTFNELKTATRNFRPDS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVRSVVDVLSCVP 180
           ++GEGGFGYV+KGWIDE TL  ++PG GMVVAVKKLK E  QGH++WL    VD L  + 
Sbjct: 121 VIGEGGFGYVYKGWIDERTLSPSKPGSGMVVAVKKLKEEGFQGHRQWLAE--VDCLGRLH 180

Query: 181 FMGFPTSFLGIKDQSGH-------------------------------------SCSQRA 240
            M      +G   +  H                                     +    A
Sbjct: 181 HMNL-VKLIGYCSKGDHIRLLVYEYMPKGSLENHLFRRGAEPIPWRTRIKVAIGAARGLA 240

Query: 241 FL------------------------------------SSRSRIARHISRFQGIEHP--T 300
           FL                                      R+ ++  +   QG   P   
Sbjct: 241 FLHEAQVIYRDFKASNILLDSEFNAKLSDFGLAKVGPTGDRTHVSTQVMGTQGYAAPEYV 300

Query: 301 RRSRLTSKSDVYSFGVVLLELLSG--------LCAEDNLVEWATPYLGDKRRLFRIMDTK 341
              R+T+KSDVYSFGVVLLELLSG        +  E NLV+WA PYLGDKR++FRIMDTK
Sbjct: 301 ATGRITAKSDVYSFGVVLLELLSGRLTVDKTKVGVERNLVDWAIPYLGDKRKVFRIMDTK 360

BLAST of Cp4.1LG02g09030 vs. TAIR 10
Match: AT1G14370.1 (protein kinase 2A )

HSP 1 Score: 221.9 bits (564), Expect = 8.5e-58
Identity = 167/438 (38.13%), Postives = 214/438 (48.86%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSAKV+ ++ S P ++SA                         GS++S  +S+ 
Sbjct: 1   MGNCLDSSAKVDNSNHS-PHANSASS-----------------------GSKVSSKTSRS 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
             P   S    S       +++   LPT RTEGEILSS NLK F FNELKNAT+NFR D+
Sbjct: 61  TGPSGLSTTSYS------TDSSFGPLPTLRTEGEILSSPNLKAFTFNELKNATKNFRQDN 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWL------------ 180
           LLGEGGFG VFKGWID+ +L A+RPG G+VVAVK+LK E  QGHKEWL            
Sbjct: 121 LLGEGGFGCVFKGWIDQTSLTASRPGSGIVVAVKQLKPEGFQGHKEWLTEVNYLGQLSHP 180

Query: 181 --------------------------------------------VRSVVDVLSCVPFMGF 240
                                                       ++  V     + F+  
Sbjct: 181 NLVLLVGYCAEGENRLLVYEFMPKGSLENHLFRRGAQPLTWAIRMKVAVGAAKGLTFLHE 240

Query: 241 PTSFLGIKD---------------QSGHSCSQRAFLSSRSRIARHISRFQGIEHP--TRR 300
             S +  +D                S    ++       + ++  +    G   P     
Sbjct: 241 AKSQVIYRDFKAANILLDADFNAKLSDFGLAKAGPTGDNTHVSTKVIGTHGYAAPEYVAT 300

Query: 301 SRLTSKSDVYSFGVVLLELLSGLCAEDN--------LVEWATPYLGDKRRLFRIMDTKLE 358
            RLT+KSDVYSFGVVLLEL+SG  A DN        LV+WATPYLGDKR+LFRIMDTKL 
Sbjct: 301 GRLTAKSDVYSFGVVLLELISGRRAMDNSNGGNEYSLVDWATPYLGDKRKLFRIMDTKLG 360

BLAST of Cp4.1LG02g09030 vs. TAIR 10
Match: AT1G69790.1 (Protein kinase superfamily protein )

HSP 1 Score: 203.8 bits (517), Expect = 2.4e-52
Identity = 148/399 (37.09%), Postives = 192/399 (48.12%), Query Frame = 0

Query: 1   MGNCFGSSAKVEIAHSSRPTSDSAVCSLKFQILYSVSKETRILSFVFVYGSEISQSSSKK 60
           MGNC  SSA+V    S                  +    +RI        S     SS+ 
Sbjct: 1   MGNCLDSSARVGNRES------------------TFGGSSRI--------SRKPNQSSRL 60

Query: 61  HNPIVPSYNPNSVVDRNDNNNNVLNLPTPRTEGEILSSSNLKPFHFNELKNATRNFRPDS 120
            +  +PSY+ NS    + +N       TPR+EGE+L S  LK F FNELK ATRNF+P+S
Sbjct: 61  SSLTIPSYSNNSFTTSSWSN------LTPRSEGELLPSPTLKAFTFNELKTATRNFKPNS 120

Query: 121 LLGEGGFGYVFKGWIDENTLGAARPGLGMVVAVKKLKAEASQGHKEWLVR----SVVDVL 180
           ++GEGGFG V+KGWI E +L  ++PG GMVVAVKKLK+E  QGHKEWL        +  +
Sbjct: 121 MIGEGGFGCVYKGWIGERSLSPSKPGSGMVVAVKKLKSEGFQGHKEWLTEVHYLGRLHHM 180

Query: 181 SCVPFMGF--------------PTSFL--------------------------GIKDQSG 240
           + V  +G+              P   L                          G+     
Sbjct: 181 NLVKLIGYCLEGEKRLLVYEYMPKGSLENHLFRRGAEPIPWKTRMKVAFSAARGLSFLHE 240

Query: 241 HSCSQRAFLSS-------------------------RSRIARHISRFQGIEHP--TRRSR 300
                R F +S                         R+ +   +   QG   P      R
Sbjct: 241 AKVIYRDFKASNILLDVDFNAKLSDFGLAKAGPTGDRTHVTTQVIGTQGYAAPEYIATGR 300

Query: 301 LTSKSDVYSFGVVLLELLSG--------LCAEDNLVEWATPYLGDKRRLFRIMDTKLEGR 321
           LTSKSDVYSFGVVLLELLSG        +  E NLV+WA PYL D+R++FRIMDTKL G+
Sbjct: 301 LTSKSDVYSFGVVLLELLSGRPTLDKSKVGVERNLVDWAIPYLVDRRKVFRIMDTKLGGQ 360

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498403.5e-6440.09Probable serine/threonine-protein kinase PBL3 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5PP292.0e-5939.01Probable serine/threonine-protein kinase PBL4 OS=Arabidopsis thaliana OX=3702 GN... [more]
O498391.2e-5638.13Probable serine/threonine-protein kinase PBL2 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q5XF793.4e-5137.09Probable serine/threonine-protein kinase PBL18 OS=Arabidopsis thaliana OX=3702 G... [more]
Q65XV86.6e-4738.02Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Match NameE-valueIdentityDescription
XP_023522677.17.64e-16665.13probable serine/threonine-protein kinase PBL3 [Cucurbita pepo subsp. pepo][more]
XP_022946188.11.67e-15763.51probable serine/threonine-protein kinase PBL3 [Cucurbita moschata][more]
XP_022999699.11.28e-15462.82probable serine/threonine-protein kinase PBL3 [Cucurbita maxima][more]
KAG7030002.13.71e-13565.29putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s... [more]
KAG6599064.16.78e-13257.27putative serine/threonine-protein kinase PBL3, partial [Cucurbita argyrosperma s... [more]
Match NameE-valueIdentityDescription
A0A6J1G3388.09e-15863.51probable serine/threonine-protein kinase PBL3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KHV06.22e-15562.82probable serine/threonine-protein kinase PBL3 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7T4R24.24e-12253.70Protein kinase 2B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00... [more]
A0A1S3C2W04.24e-12253.70protein kinase 2B, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103496405 PE... [more]
A0A0A0KE202.25e-11952.93Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G404... [more]
Match NameE-valueIdentityDescription
AT2G02800.12.5e-6540.09protein kinase 2B [more]
AT2G02800.22.5e-6540.09protein kinase 2B [more]
AT1G26970.11.4e-6039.01Protein kinase superfamily protein [more]
AT1G14370.18.5e-5838.13protein kinase 2A [more]
AT1G69790.12.4e-5237.09Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 205..340
e-value: 5.1E-19
score: 70.3
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 87..180
e-value: 2.6E-14
score: 55.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 321..358
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 324..358
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 81..169
NoneNo IPR availablePANTHERPTHR45621:SF109PROTEIN KINASE 2B, CHLOROPLASTICcoord: 227..348
coord: 81..169
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 227..348
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 227..314
e-value: 4.1E-6
score: 26.3
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 122..155
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..318
score: 8.428824
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 228..322
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 93..170

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g09030.1Cp4.1LG02g09030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity