Cp4.1LG02g08140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g08140 vs. ExPASy Swiss-Prot
Match: F4I0P8 (Vacuolar protein sorting-associated protein 35B OS=Arabidopsis thaliana OX=3702 GN=VPS35B PE=1 SV=1)

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 591/796 (74.25%), Postives = 679/796 (85.30%), Query Frame = 0

Query: 5   GIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELY 64
           G+EDE+KWLAEGIAGIQHNAF+MH+A+DANNLRE LKYSA MLSELRTSKLSP +YY+LY
Sbjct: 6   GVEDEDKWLAEGIAGIQHNAFFMHRALDANNLREVLKYSALMLSELRTSKLSPQKYYDLY 65

Query: 65  MRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAK 124
           MRAFD+LR LEIFFKDESRHG+ VVDLYELVQHAGN+LPR+YLLCTVGSVY+K+K+ P+K
Sbjct: 66  MRAFDQLRQLEIFFKDESRHGLPVVDLYELVQHAGNILPRMYLLCTVGSVYIKSKQAPSK 125

Query: 125 EVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVLQNFT 184
           +VLKDLVEMCRGVQHPIRGLFLR+YLAQVSRD L +I S+ EGDA+TVM+AVEFVLQNFT
Sbjct: 126 DVLKDLVEMCRGVQHPIRGLFLRSYLAQVSRDKLPEIGSDYEGDANTVMDAVEFVLQNFT 185

Query: 185 EMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRVLE 244
           EMNKLWVRIQ QGP  +REKQEKER ELRDLVGKNLHVL QIEGVDLEMYK++VLPRVLE
Sbjct: 186 EMNKLWVRIQHQGPGTVREKQEKERNELRDLVGKNLHVLGQIEGVDLEMYKETVLPRVLE 245

Query: 245 QVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQL------------------- 304
           QVVNCKD+LAQYYLM+CIIQVFPDEYHLQTLETLL  C QL                   
Sbjct: 246 QVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSN 305

Query: 305 --------------------------QVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLD 364
                                     +VI+ Q++MPIVGA+TL+VSLLTFTLRVHPDRLD
Sbjct: 306 YAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLD 365

Query: 365 YVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMDHLDN 424
           YVDQ+LGACV  LSS PKLED RA KQ+V LLSAPLEKY+DIVTALTLSNYPRVMDHLD+
Sbjct: 366 YVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDD 425

Query: 425 ATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKEEQNS 484
            TNKVMAM+IIQSIM ++SCISTADKV+VLFELIKGLIKDL+E   +ELDEEDF+EEQNS
Sbjct: 426 GTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNS 485

Query: 485 VARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQDRDV 544
           VARL+HML N++P EMLKIIC V++H+++GGP RLPFTVPPL+FSA+ L+R+L++Q  D+
Sbjct: 486 VARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDI 545

Query: 545 VGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDCDLEP 604
            GE+            ATP+KIFQ+LNQ IE L+SVP PELALRLYL+CAEAA+DCDLEP
Sbjct: 546 AGEDS-----------ATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEP 605

Query: 605 VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYSAKLL 664
           VAYEFFTQAF++YEE+IADSKAQVTAIHLI+GTLQ++NVFG+ENRDTLTHKATGYSA+LL
Sbjct: 606 VAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLL 665

Query: 665 KKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGGPITL 724
           KKPDQC+AVYACSHLFWVDDP+GIKDGERVLLCL+RALRIANAAQQMA+ TRGS GP+TL
Sbjct: 666 KKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTL 725

Query: 725 FVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRYIQFQ 756
           FVEILNKY+++FEKGN  IT + IQ LIELI  E+ +D  +    SD FFTSTLRYI+F 
Sbjct: 726 FVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHSDPFFTSTLRYIKFI 785

BLAST of Cp4.1LG02g08140 vs. ExPASy Swiss-Prot
Match: Q7X659 (Vacuolar protein sorting-associated protein 35A OS=Arabidopsis thaliana OX=3702 GN=VPS35A PE=1 SV=1)

HSP 1 Score: 1043.9 bits (2698), Expect = 8.9e-304
Identity = 523/800 (65.38%), Postives = 642/800 (80.25%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           M++ G EDEEKWLA G A  + NAFYM +A+D+NNL++ALKYSAQMLSELRTSKLSPH+Y
Sbjct: 1   MIADGSEDEEKWLAAGAAAFKQNAFYMQRAIDSNNLKDALKYSAQMLSELRTSKLSPHKY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           Y+LYMRAFDELR LEIFF +E+R G  V++LYELVQHAGN+LPRLYLLCT GSVY+KTKE
Sbjct: 61  YDLYMRAFDELRKLEIFFMEETRRGCSVIELYELVQHAGNILPRLYLLCTAGSVYIKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
            PAKE+LKDLVEMCRG+QHP+RGLFLR+YLAQ+SRD L DI SE EGDADTV++AVEFVL
Sbjct: 121 APAKEILKDLVEMCRGIQHPLRGLFLRSYLAQISRDKLPDIGSEYEGDADTVIDAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
            NFTEMNKLWVR+Q QGPA  +E++EKER ELRDLVGKNLHVLSQ+EGVDL+MY+ +VLP
Sbjct: 181 LNFTEMNKLWVRMQHQGPAREKERREKERGELRDLVGKNLHVLSQLEGVDLDMYRDTVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQ+VNC+DE+AQYYL+DCIIQVFPDEYHLQTL+ LLG CPQLQ              
Sbjct: 241 RVLEQIVNCRDEIAQYYLIDCIIQVFPDEYHLQTLDVLLGACPQLQASVDIMTVLSRLME 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQ DMPI+ A+TLY SLL FTL VHP
Sbjct: 301 RLSNYAALNAEVLPYFLQVEAFSKLNNAIGKVIEAQEDMPILSAVTLYSSLLKFTLHVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDY DQ+LG+CVK LS + K++D RATK++V LLSAPLEKYND+VTAL L+NYP V++
Sbjct: 361 DRLDYADQVLGSCVKQLSGKGKIDDTRATKELVSLLSAPLEKYNDVVTALKLTNYPLVVE 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           +LD  T ++MA VI++SIM +N+ I+TA+KV+ LFELIKG+I DL+E    E+DE+DF+E
Sbjct: 421 YLDTETKRIMATVIVRSIMKNNTLITTAEKVEALFELIKGIINDLDEPQGLEVDEDDFQE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVA L+HML+NDDP EM KI+  +KKH L+GGP RL FT+PPL+ S L LIRRL  +
Sbjct: 481 EQNSVALLIHMLYNDDPEEMFKIVNVLKKHFLTGGPKRLKFTIPPLVVSTLKLIRRLPVE 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
             +  G+E S           T  KIFQ LNQ+IEAL +VP+P+LA RLYL+CAEAA+ C
Sbjct: 541 GDNPFGKEAS----------VTATKIFQFLNQIIEALPNVPSPDLAFRLYLQCAEAADKC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           D EP+AYEFFTQA+I+YEE+I+DSKAQVTA+ LIIGTLQ+M VFG+ENRDTLTHKATGY+
Sbjct: 601 DEEPIAYEFFTQAYILYEEEISDSKAQVTALQLIIGTLQRMQVFGVENRDTLTHKATGYA 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFW++D E I+DGERVLLCLKRAL+IAN+AQQ+AN  RGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWLEDRETIQDGERVLLCLKRALKIANSAQQVANTARGSTG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 756
            +TLF+EILNKYL+++EKG  QIT  +++ LI+LI+ E   ++  ++P +++FF +TL +
Sbjct: 721 SVTLFIEILNKYLYFYEKGVPQITVESVESLIKLIKNE---ESMPSDPSAESFFATTLEF 780

BLAST of Cp4.1LG02g08140 vs. ExPASy Swiss-Prot
Match: A8R7K9 (Vacuolar protein sorting-associated protein 35C OS=Arabidopsis thaliana OX=3702 GN=VPS35C PE=2 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 9.6e-282
Identity = 491/797 (61.61%), Postives = 616/797 (77.29%), Query Frame = 0

Query: 7   EDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELYMR 66
           +D+EKWLA  IA ++ NAFYM +A+D+NNL++ALK+SAQMLSELRTSKLSPH+YYELYMR
Sbjct: 4   DDDEKWLAAAIAAVKQNAFYMQRAIDSNNLKDALKFSAQMLSELRTSKLSPHKYYELYMR 63

Query: 67  AFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAKEV 126
            F+EL  LEIFFK+E+  G  + +LYELVQHAGN+LPRLYLLCT+GSVY+K+K+V A ++
Sbjct: 64  VFNELGTLEIFFKEETGRGCSIAELYELVQHAGNILPRLYLLCTIGSVYIKSKDVTATDI 123

Query: 127 LKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVLQNFTEM 186
           LKDLVEMCR VQHP+RGLFLR+YLAQV+RD L  I S+ EGD D  M A+EFVLQNFTEM
Sbjct: 124 LKDLVEMCRAVQHPLRGLFLRSYLAQVTRDKLPSIGSDLEGDGDAHMNALEFVLQNFTEM 183

Query: 187 NKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRVLEQV 246
           NKLWVR+Q QGP+  +EK+EKER ELRDLVGKNLHVLSQ+EGVDL +Y+ +VLPR+LEQV
Sbjct: 184 NKLWVRMQHQGPSREKEKREKERNELRDLVGKNLHVLSQLEGVDLGIYRDTVLPRILEQV 243

Query: 247 VNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------------- 306
           VNCKDELAQ YLMDCIIQVFPD++HLQTL+ LLG CPQLQ                    
Sbjct: 244 VNCKDELAQCYLMDCIIQVFPDDFHLQTLDVLLGACPQLQPSVDIKTVLSGLMERLSNYA 303

Query: 307 -------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLDYV 366
                                    V+EAQ D+P   ++TLY+ LL FTL V+ DRLDYV
Sbjct: 304 ASSVEALPNFLQVEAFSKLNYAIGKVVEAQADLPAAASVTLYLFLLKFTLHVYSDRLDYV 363

Query: 367 DQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMDHLDNAT 426
           DQ+LG+CV  LS+  KL D +A KQIV  LSAPLEKYN++VT L L+NYP VM++LD  T
Sbjct: 364 DQVLGSCVTQLSATGKLCDDKAAKQIVAFLSAPLEKYNNVVTILKLTNYPLVMEYLDRET 423

Query: 427 NKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKEEQNSVA 486
           NK MA++++QS+  +N+ I+TAD+VD LFEL KGL+KD +  I DE+DEEDF+EEQN VA
Sbjct: 424 NKAMAIILVQSVFKNNTHIATADEVDALFELAKGLMKDFDGTIDDEIDEEDFQEEQNLVA 483

Query: 487 RLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQDRDVVG 546
           RL++ L+ DDP EM KII  V+KHI++GGP RLP T+PPL+FSAL LIRRL+  D +  G
Sbjct: 484 RLVNKLYIDDPEEMSKIIFTVRKHIVAGGPKRLPLTIPPLVFSALKLIRRLRGGDENPFG 543

Query: 547 EEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDCDLEPVA 606
           ++ S          ATPK+I QLL++ +E LS V AP+LALRLYL+CA+AAN+C+LE VA
Sbjct: 544 DDAS----------ATPKRILQLLSETVEVLSDVSAPDLALRLYLQCAQAANNCELETVA 603

Query: 607 YEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYSAKLLKK 666
           YEFFT+A+++YEE+I+DSKAQVTA+ LIIGTLQ+M VF +ENRDTLTHKATGYSA+LL+K
Sbjct: 604 YEFFTKAYLLYEEEISDSKAQVTALRLIIGTLQRMRVFNVENRDTLTHKATGYSARLLRK 663

Query: 667 PDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRG--SGGPITL 726
           PDQC+AVY C+HLFW D+ E +KDGERV+LCLKRA RIA+A QQMAN +RG  S G ++L
Sbjct: 664 PDQCRAVYECAHLFWADECENLKDGERVVLCLKRAQRIADAVQQMANASRGTSSTGSVSL 723

Query: 727 FVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRYIQFQ 756
           +VE+LNKYL++ EKGNQQ+T   I+ L ELI++E    T      ++ F  STLRYI+FQ
Sbjct: 724 YVELLNKYLYFLEKGNQQVTGDTIKSLAELIKSE----TKKVESGAEPFINSTLRYIEFQ 783

BLAST of Cp4.1LG02g08140 vs. ExPASy Swiss-Prot
Match: Q2HJG5 (Vacuolar protein sorting-associated protein 35 OS=Bos taurus OX=9913 GN=VPS35 PE=2 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 1.4e-160
Identity = 334/808 (41.34%), Postives = 486/808 (60.15%), Query Frame = 0

Query: 7   EDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELYMR 66
           +++EK L E I  ++  +F M + +D N L +ALK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 67  AFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAKEV 126
             DEL  LE++  DE   G  V DLYELVQ+AGN++PRLYLL TVG VY+K+     K++
Sbjct: 70  ISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDI 129

Query: 127 LKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLD----INSECEGDADTVMEAVEFVLQN 186
           LKDLVEMCRGVQHP+RGLFLRNYL Q +R+IL D     + E  GD   + ++++FVL N
Sbjct: 130 LKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGD---ISDSMDFVLLN 189

Query: 187 FTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRV 246
           F EMNKLWVR+Q QG +  REK+E+ER ELR LVG NL  LSQ+EGV++E YKQ VL  +
Sbjct: 190 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 249

Query: 247 LEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ---------------- 306
           LEQVVNC+D LAQ YLM+CIIQVFPDE+HLQTL   L  C +L                 
Sbjct: 250 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 309

Query: 307 ----------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHPDRL 366
                                       VI+++ DMP    ++L VSL+   ++ +PDR+
Sbjct: 310 ALFAHREDGPGIPTDIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRV 369

Query: 367 DYVDQILGACVKTLSSQPKLEDI----RATKQIVILLSAPLEKYNDIVTALTLSNYPRVM 426
           DYVD++L   V+ + ++  LE I      +K++  LL  P++ YN+I+T L L ++  + 
Sbjct: 370 DYVDKVLETTVE-IFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLF 429

Query: 427 DHLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFK 486
           ++ D  + K M+  ++ ++++ N+ I + D+VD +  L+  LI+D  +  V+E D EDF 
Sbjct: 430 EYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEEPDPEDFA 489

Query: 487 EEQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQN 546
           +EQ+ V R +H+L ++DP +   I+   +KH  +GG  R+ FT+PPL+F+A  L  R   
Sbjct: 490 DEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRY-- 549

Query: 547 QDRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAAND 606
                  +E S+  D  E+     +KIF   +Q I AL      EL LRL+L+ A AA +
Sbjct: 550 -------KENSKVDDKWEK---KCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGE 609

Query: 607 CDL---EPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKA 666
                 E VAYEF +QAF +YE++I+DSKAQ+ AI LIIGT ++M  F  EN + L  + 
Sbjct: 610 IGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQC 669

Query: 667 TGYSAKLLKKPDQCKAVYACSHLFWV-----DDPEGIKDGERVLLCLKRALRIANAAQQM 726
              ++KLLKKPDQ +AV  C+HLFW       + E +  G+RV+ CLK+AL+IAN     
Sbjct: 670 ALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDP 729

Query: 727 ANVTRGSGGPITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLT-DTPSANPPS 754
           +         + LF+EILN+Y++++EK N  +T   +  LI+ IR +L   ++       
Sbjct: 730 SL-------QVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQI 789

BLAST of Cp4.1LG02g08140 vs. ExPASy Swiss-Prot
Match: Q9EQH3 (Vacuolar protein sorting-associated protein 35 OS=Mus musculus OX=10090 GN=Vps35 PE=1 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 1.9e-160
Identity = 334/808 (41.34%), Postives = 486/808 (60.15%), Query Frame = 0

Query: 7   EDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELYMR 66
           +++EK L E I  ++  +F M + +D N L +ALK+++ ML ELRTS LSP  YYELYM 
Sbjct: 10  DEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMA 69

Query: 67  AFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAKEV 126
             DEL  LE++  DE   G  V DLYELVQ+AGN++PRLYLL TVG VY+K+     K++
Sbjct: 70  ISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDI 129

Query: 127 LKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLD----INSECEGDADTVMEAVEFVLQN 186
           LKDLVEMCRGVQHP+RGLFLRNYL Q +R+IL D     + E  GD   + ++++FVL N
Sbjct: 130 LKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGD---ISDSMDFVLLN 189

Query: 187 FTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRV 246
           F EMNKLWVR+Q QG +  REK+E+ER ELR LVG NL  LSQ+EGV++E YKQ VL  +
Sbjct: 190 FAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGI 249

Query: 247 LEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ---------------- 306
           LEQVVNC+D LAQ YLM+CIIQVFPDE+HLQTL   L  C +L                 
Sbjct: 250 LEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRL 309

Query: 307 ----------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHPDRL 366
                                       VI+++ DMP    ++L VSL+   ++ +PDR+
Sbjct: 310 ALFAHREDGPGIPAEIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRV 369

Query: 367 DYVDQILGACVKTLSSQPKLEDI----RATKQIVILLSAPLEKYNDIVTALTLSNYPRVM 426
           DYVD++L   V+ + ++  LE I      +K++  LL  P++ YN+I+T L L ++  + 
Sbjct: 370 DYVDKVLETTVE-IFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLF 429

Query: 427 DHLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFK 486
           ++ D  + K M+  ++ ++++ N+ I + D+VD +  L+  LI+D  +  V++ D EDF 
Sbjct: 430 EYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFA 489

Query: 487 EEQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQN 546
           +EQ+ V R +H+L +DDP +   I+   +KH  +GG  R+ FT+PPL+F+A  L  R   
Sbjct: 490 DEQSLVGRFIHLLRSDDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRY-- 549

Query: 547 QDRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAAND 606
                  +E S+  D  E+     +KIF   +Q I AL      EL LRL+L+ A AA +
Sbjct: 550 -------KENSQMDDKWEK---KCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGE 609

Query: 607 CDL---EPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKA 666
                 E VAYEF +QAF +YE++I+DSKAQ+ AI LIIGT ++M  F  EN + L  + 
Sbjct: 610 IGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQC 669

Query: 667 TGYSAKLLKKPDQCKAVYACSHLFWV-----DDPEGIKDGERVLLCLKRALRIANAAQQM 726
              ++KLLKKPDQ +AV  C+HLFW       + E +  G+RV+ CLK+AL+IAN     
Sbjct: 670 ALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDP 729

Query: 727 ANVTRGSGGPITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLT-DTPSANPPS 754
           +         + LF+EILN+Y++++EK N  +T   +  LI+ IR +L   ++       
Sbjct: 730 SL-------QVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQI 789

BLAST of Cp4.1LG02g08140 vs. NCBI nr
Match: XP_023525738.1 (vacuolar protein sorting-associated protein 35B-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1446 bits (3744), Expect = 0.0
Identity = 755/800 (94.38%), Postives = 755/800 (94.38%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. NCBI nr
Match: XP_022941400.1 (vacuolar protein sorting-associated protein 35B-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 749/800 (93.62%), Postives = 752/800 (94.00%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLC+VGSVYMKTK+
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCSVGSVYMKTKD 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLED RATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEI VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKIICAVKKHIL GGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLY+ECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYMECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. NCBI nr
Match: KAG6608605.1 (Vacuolar protein sorting-associated protein 35B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 750/800 (93.75%), Postives = 751/800 (93.88%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLED RATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEI VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKIICAVKKHIL GGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLY+ECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYMECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTEL TDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELPTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. NCBI nr
Match: XP_022982498.1 (vacuolar protein sorting-associated protein 35B-like [Cucurbita maxima])

HSP 1 Score: 1432 bits (3708), Expect = 0.0
Identity = 749/800 (93.62%), Postives = 750/800 (93.75%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEF+L
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFML 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLED RATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEI VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKII AVKKHIL GGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIIYAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. NCBI nr
Match: XP_022131851.1 (vacuolar protein sorting-associated protein 35B-like [Momordica charantia])

HSP 1 Score: 1378 bits (3566), Expect = 0.0
Identity = 714/800 (89.25%), Postives = 735/800 (91.88%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGVDLEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVDLEMYKQAVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKNLSSQPKLEDTRATKQIVMLLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIM +NSCISTADKV+VLFELIKGLIKDLEE+  DELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVEVLFELIKGLIKDLEEVTADELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDP+EMLKIIC VKKHIL GGP+RLPFTVPPLI SALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPVEMLKIICTVKKHILCGGPNRLPFTVPPLILSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVV EEQ E+GDV EEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVREEQGESGDVEEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEE+IADSKAQVT+IHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEEIADSKAQVTSIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFWVD+PEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDEPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PS  P SDAFFTSTLRY
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSTTPASDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. ExPASy TrEMBL
Match: A0A6J1FTJ5 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita moschata OX=3662 GN=LOC111446704 PE=3 SV=1)

HSP 1 Score: 1437 bits (3720), Expect = 0.0
Identity = 749/800 (93.62%), Postives = 752/800 (94.00%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLC+VGSVYMKTK+
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCSVGSVYMKTKD 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLED RATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEI VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKIICAVKKHIL GGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLY+ECAEAANDC
Sbjct: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYMECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. ExPASy TrEMBL
Match: A0A6J1J4Z2 (Vacuolar protein sorting-associated protein 35 OS=Cucurbita maxima OX=3661 GN=LOC111481301 PE=3 SV=1)

HSP 1 Score: 1432 bits (3708), Expect = 0.0
Identity = 749/800 (93.62%), Postives = 750/800 (93.75%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEF+L
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFML 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPDFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVKTLSSQPKLED RATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKTLSSQPKLEDTRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEI VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEITVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDPLEMLKII AVKKHIL GGPSRLPFTVPPLIFSALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPLEMLKIIYAVKKHILCGGPSRLPFTVPPLIFSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG
Sbjct: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY
Sbjct: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. ExPASy TrEMBL
Match: A0A6J1BQM1 (Vacuolar protein sorting-associated protein 35 OS=Momordica charantia OX=3673 GN=LOC111004898 PE=3 SV=1)

HSP 1 Score: 1378 bits (3566), Expect = 0.0
Identity = 714/800 (89.25%), Postives = 735/800 (91.88%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGVDLEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVDLEMYKQAVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAASNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYNDIVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKNLSSQPKLEDTRATKQIVMLLSAPLEKYNDIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIM +NSCISTADKV+VLFELIKGLIKDLEE+  DELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVEVLFELIKGLIKDLEEVTADELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDP+EMLKIIC VKKHIL GGP+RLPFTVPPLI SALSLIRRLQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPVEMLKIICTVKKHILCGGPNRLPFTVPPLILSALSLIRRLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVV EEQ E+GDV EEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVREEQGESGDVEEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEE+IADSKAQVT+IHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEEIADSKAQVTSIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFWVD+PEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDEPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 755
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PS  P SDAFFTSTLRY
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSTTPASDAFFTSTLRY 780

BLAST of Cp4.1LG02g08140 vs. ExPASy TrEMBL
Match: A0A1S3CSM2 (Vacuolar protein sorting-associated protein 35 OS=Cucumis melo OX=3656 GN=LOC103503864 PE=3 SV=1)

HSP 1 Score: 1365 bits (3532), Expect = 0.0
Identity = 712/802 (88.78%), Postives = 733/802 (91.40%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPA LREKQEKER ELRDLVGKNLHVLSQIEGVDLEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERNELRDLVGKNLHVLSQIEGVDLEMYKQTVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVK LSSQPKLED RATKQIV+LLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKLEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIM +NSCISTADKVDVLFELIKGLIKDLEE+ VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEVSVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDP EMLKIICAVKKHIL GGP+RLPFTVPPLIFSAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICAVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVVGEEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPS--DAFFTSTL 755
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PSANP    DAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

BLAST of Cp4.1LG02g08140 vs. ExPASy TrEMBL
Match: A0A0A0LE86 (Vacuolar protein sorting-associated protein 35 OS=Cucumis sativus OX=3659 GN=Csa_3G873280 PE=3 SV=1)

HSP 1 Score: 1357 bits (3513), Expect = 0.0
Identity = 708/802 (88.28%), Postives = 731/802 (91.15%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           MLS+GIEDEEKWLAEGIAGIQHNAFYMHQA+DAN+LRE LKYSAQMLSELRTSKLSPHRY
Sbjct: 1   MLSVGIEDEEKWLAEGIAGIQHNAFYMHQAMDANSLREGLKYSAQMLSELRTSKLSPHRY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           YELYMRAFDELRMLEIFFKDESRHGV VVDLYELVQHAGN+LPRLYLLCTVGSVYMK+KE
Sbjct: 61  YELYMRAFDELRMLEIFFKDESRHGVTVVDLYELVQHAGNILPRLYLLCTVGSVYMKSKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
           VPAKEVLKDLVEMCRGVQHPIRGLFLR+YLAQVSRDILLDINSE EGDADTVMEAVEFVL
Sbjct: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRHYLAQVSRDILLDINSEGEGDADTVMEAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
           QNFTEMNKLWVRIQLQGPA LREKQEKER+ELRDLVGKNLHVLSQIEGV+LEMYKQ+VLP
Sbjct: 181 QNFTEMNKLWVRIQLQGPARLREKQEKERSELRDLVGKNLHVLSQIEGVNLEMYKQTVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQVVNCKDELAQYYLM+CIIQVFPDEYHLQTLETLLGVCPQLQ              
Sbjct: 241 RVLEQVVNCKDELAQYYLMECIIQVFPDEYHLQTLETLLGVCPQLQATVDIRMVLSQLMD 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQVDMPIVGAITLYVSLLTFTLRVHP
Sbjct: 301 RLSNYAVSNADVIPEFLHVEAFAKLSNAIGKVIEAQVDMPIVGAITLYVSLLTFTLRVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDYVDQILGACVK LSSQPK+ED RATKQIV+LLSAPLEKYN IVTALTLSNYPRVMD
Sbjct: 361 DRLDYVDQILGACVKKLSSQPKIEDARATKQIVMLLSAPLEKYNGIVTALTLSNYPRVMD 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           HLDNATNKVMAMVIIQSIM +NSCISTADKVDVLFELIKGLIKDLEE  VDELDEEDFKE
Sbjct: 421 HLDNATNKVMAMVIIQSIMKNNSCISTADKVDVLFELIKGLIKDLEEASVDELDEEDFKE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVARLLHMLHNDDP EMLKIIC VKKHIL GGP+RLPFTVPPLIFSAL+LIR+LQNQ
Sbjct: 481 EQNSVARLLHMLHNDDPGEMLKIICTVKKHILCGGPNRLPFTVPPLIFSALNLIRQLQNQ 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
           D DVVGEEQ E GD  EEMHATPKKIFQ+LNQLIEALSSVPAPELALRLYLECAEAANDC
Sbjct: 541 DGDVVGEEQHEVGDEAEEMHATPKKIFQILNQLIEALSSVPAPELALRLYLECAEAANDC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQ+MNVFG+ENRDTLTHKATGYS
Sbjct: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQRMNVFGVENRDTLTHKATGYS 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSSG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPS--DAFFTSTL 755
           P+TLFVEILNKYL+YFEKGNQQITNAAIQGLIELI TEL TD+PSANP    DAFFTSTL
Sbjct: 721 PVTLFVEILNKYLYYFEKGNQQITNAAIQGLIELITTELQTDSPSANPAPAPDAFFTSTL 780

BLAST of Cp4.1LG02g08140 vs. TAIR 10
Match: AT1G75850.1 (VPS35 homolog B )

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 591/796 (74.25%), Postives = 679/796 (85.30%), Query Frame = 0

Query: 5   GIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELY 64
           G+EDE+KWLAEGIAGIQHNAF+MH+A+DANNLRE LKYSA MLSELRTSKLSP +YY+LY
Sbjct: 6   GVEDEDKWLAEGIAGIQHNAFFMHRALDANNLREVLKYSALMLSELRTSKLSPQKYYDLY 65

Query: 65  MRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAK 124
           MRAFD+LR LEIFFKDESRHG+ VVDLYELVQHAGN+LPR+YLLCTVGSVY+K+K+ P+K
Sbjct: 66  MRAFDQLRQLEIFFKDESRHGLPVVDLYELVQHAGNILPRMYLLCTVGSVYIKSKQAPSK 125

Query: 125 EVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVLQNFT 184
           +VLKDLVEMCRGVQHPIRGLFLR+YLAQVSRD L +I S+ EGDA+TVM+AVEFVLQNFT
Sbjct: 126 DVLKDLVEMCRGVQHPIRGLFLRSYLAQVSRDKLPEIGSDYEGDANTVMDAVEFVLQNFT 185

Query: 185 EMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRVLE 244
           EMNKLWVRIQ QGP  +REKQEKER ELRDLVGKNLHVL QIEGVDLEMYK++VLPRVLE
Sbjct: 186 EMNKLWVRIQHQGPGTVREKQEKERNELRDLVGKNLHVLGQIEGVDLEMYKETVLPRVLE 245

Query: 245 QVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQL------------------- 304
           QVVNCKD+LAQYYLM+CIIQVFPDEYHLQTLETLL  C QL                   
Sbjct: 246 QVVNCKDKLAQYYLMECIIQVFPDEYHLQTLETLLAACTQLMPTVDTKIVLTQLMDRLSN 305

Query: 305 --------------------------QVIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLD 364
                                     +VI+ Q++MPIVGA+TL+VSLLTFTLRVHPDRLD
Sbjct: 306 YAASSPDVLHEFLQVEAFAKLSNAIGKVIDTQLEMPIVGAMTLFVSLLTFTLRVHPDRLD 365

Query: 365 YVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMDHLDN 424
           YVDQ+LGACV  LSS PKLED RA KQ+V LLSAPLEKY+DIVTALTLSNYPRVMDHLD+
Sbjct: 366 YVDQVLGACVVKLSSVPKLEDARAMKQVVALLSAPLEKYSDIVTALTLSNYPRVMDHLDD 425

Query: 425 ATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKEEQNS 484
            TNKVMAM+IIQSIM ++SCISTADKV+VLFELIKGLIKDL+E   +ELDEEDF+EEQNS
Sbjct: 426 GTNKVMAMLIIQSIMKTDSCISTADKVEVLFELIKGLIKDLDETNAEELDEEDFQEEQNS 485

Query: 485 VARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQDRDV 544
           VARL+HML N++P EMLKIIC V++H+++GGP RLPFTVPPL+FSA+ L+R+L++Q  D+
Sbjct: 486 VARLIHMLDNEEPEEMLKIICVVRRHLMTGGPRRLPFTVPPLVFSAVRLVRQLESQGGDI 545

Query: 545 VGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDCDLEP 604
            GE+            ATP+KIFQ+LNQ IE L+SVP PELALRLYL+CAEAA+DCDLEP
Sbjct: 546 AGEDS-----------ATPRKIFQILNQTIEVLTSVPCPELALRLYLQCAEAASDCDLEP 605

Query: 605 VAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYSAKLL 664
           VAYEFFTQAF++YEE+IADSKAQVTAIHLI+GTLQ++NVFG+ENRDTLTHKATGYSA+LL
Sbjct: 606 VAYEFFTQAFMLYEEEIADSKAQVTAIHLIVGTLQRINVFGVENRDTLTHKATGYSARLL 665

Query: 665 KKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGGPITL 724
           KKPDQC+AVYACSHLFWVDDP+GIKDGERVLLCL+RALRIANAAQQMA+ TRGS GP+TL
Sbjct: 666 KKPDQCRAVYACSHLFWVDDPDGIKDGERVLLCLRRALRIANAAQQMASATRGSSGPVTL 725

Query: 725 FVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRYIQFQ 756
           FVEILNKY+++FEKGN  IT + IQ LIELI  E+ +D  +    SD FFTSTLRYI+F 
Sbjct: 726 FVEILNKYIYFFEKGNPHITPSDIQSLIELINNEMQSDNGNTTIHSDPFFTSTLRYIKFI 785

BLAST of Cp4.1LG02g08140 vs. TAIR 10
Match: AT2G17790.1 (VPS35 homolog A )

HSP 1 Score: 1043.9 bits (2698), Expect = 6.3e-305
Identity = 523/800 (65.38%), Postives = 642/800 (80.25%), Query Frame = 0

Query: 1   MLSIGIEDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRY 60
           M++ G EDEEKWLA G A  + NAFYM +A+D+NNL++ALKYSAQMLSELRTSKLSPH+Y
Sbjct: 1   MIADGSEDEEKWLAAGAAAFKQNAFYMQRAIDSNNLKDALKYSAQMLSELRTSKLSPHKY 60

Query: 61  YELYMRAFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKE 120
           Y+LYMRAFDELR LEIFF +E+R G  V++LYELVQHAGN+LPRLYLLCT GSVY+KTKE
Sbjct: 61  YDLYMRAFDELRKLEIFFMEETRRGCSVIELYELVQHAGNILPRLYLLCTAGSVYIKTKE 120

Query: 121 VPAKEVLKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVL 180
            PAKE+LKDLVEMCRG+QHP+RGLFLR+YLAQ+SRD L DI SE EGDADTV++AVEFVL
Sbjct: 121 APAKEILKDLVEMCRGIQHPLRGLFLRSYLAQISRDKLPDIGSEYEGDADTVIDAVEFVL 180

Query: 181 QNFTEMNKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLP 240
            NFTEMNKLWVR+Q QGPA  +E++EKER ELRDLVGKNLHVLSQ+EGVDL+MY+ +VLP
Sbjct: 181 LNFTEMNKLWVRMQHQGPAREKERREKERGELRDLVGKNLHVLSQLEGVDLDMYRDTVLP 240

Query: 241 RVLEQVVNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------- 300
           RVLEQ+VNC+DE+AQYYL+DCIIQVFPDEYHLQTL+ LLG CPQLQ              
Sbjct: 241 RVLEQIVNCRDEIAQYYLIDCIIQVFPDEYHLQTLDVLLGACPQLQASVDIMTVLSRLME 300

Query: 301 -------------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHP 360
                                          VIEAQ DMPI+ A+TLY SLL FTL VHP
Sbjct: 301 RLSNYAALNAEVLPYFLQVEAFSKLNNAIGKVIEAQEDMPILSAVTLYSSLLKFTLHVHP 360

Query: 361 DRLDYVDQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMD 420
           DRLDY DQ+LG+CVK LS + K++D RATK++V LLSAPLEKYND+VTAL L+NYP V++
Sbjct: 361 DRLDYADQVLGSCVKQLSGKGKIDDTRATKELVSLLSAPLEKYNDVVTALKLTNYPLVVE 420

Query: 421 HLDNATNKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKE 480
           +LD  T ++MA VI++SIM +N+ I+TA+KV+ LFELIKG+I DL+E    E+DE+DF+E
Sbjct: 421 YLDTETKRIMATVIVRSIMKNNTLITTAEKVEALFELIKGIINDLDEPQGLEVDEDDFQE 480

Query: 481 EQNSVARLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQ 540
           EQNSVA L+HML+NDDP EM KI+  +KKH L+GGP RL FT+PPL+ S L LIRRL  +
Sbjct: 481 EQNSVALLIHMLYNDDPEEMFKIVNVLKKHFLTGGPKRLKFTIPPLVVSTLKLIRRLPVE 540

Query: 541 DRDVVGEEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDC 600
             +  G+E S           T  KIFQ LNQ+IEAL +VP+P+LA RLYL+CAEAA+ C
Sbjct: 541 GDNPFGKEAS----------VTATKIFQFLNQIIEALPNVPSPDLAFRLYLQCAEAADKC 600

Query: 601 DLEPVAYEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYS 660
           D EP+AYEFFTQA+I+YEE+I+DSKAQVTA+ LIIGTLQ+M VFG+ENRDTLTHKATGY+
Sbjct: 601 DEEPIAYEFFTQAYILYEEEISDSKAQVTALQLIIGTLQRMQVFGVENRDTLTHKATGYA 660

Query: 661 AKLLKKPDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRGSGG 720
           AKLLKKPDQC+AVYACSHLFW++D E I+DGERVLLCLKRAL+IAN+AQQ+AN  RGS G
Sbjct: 661 AKLLKKPDQCRAVYACSHLFWLEDRETIQDGERVLLCLKRALKIANSAQQVANTARGSTG 720

Query: 721 PITLFVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRY 756
            +TLF+EILNKYL+++EKG  QIT  +++ LI+LI+ E   ++  ++P +++FF +TL +
Sbjct: 721 SVTLFIEILNKYLYFYEKGVPQITVESVESLIKLIKNE---ESMPSDPSAESFFATTLEF 780

BLAST of Cp4.1LG02g08140 vs. TAIR 10
Match: AT3G51310.1 (VPS35 homolog C )

HSP 1 Score: 970.7 bits (2508), Expect = 6.8e-283
Identity = 491/797 (61.61%), Postives = 616/797 (77.29%), Query Frame = 0

Query: 7   EDEEKWLAEGIAGIQHNAFYMHQAVDANNLREALKYSAQMLSELRTSKLSPHRYYELYMR 66
           +D+EKWLA  IA ++ NAFYM +A+D+NNL++ALK+SAQMLSELRTSKLSPH+YYELYMR
Sbjct: 4   DDDEKWLAAAIAAVKQNAFYMQRAIDSNNLKDALKFSAQMLSELRTSKLSPHKYYELYMR 63

Query: 67  AFDELRMLEIFFKDESRHGVMVVDLYELVQHAGNLLPRLYLLCTVGSVYMKTKEVPAKEV 126
            F+EL  LEIFFK+E+  G  + +LYELVQHAGN+LPRLYLLCT+GSVY+K+K+V A ++
Sbjct: 64  VFNELGTLEIFFKEETGRGCSIAELYELVQHAGNILPRLYLLCTIGSVYIKSKDVTATDI 123

Query: 127 LKDLVEMCRGVQHPIRGLFLRNYLAQVSRDILLDINSECEGDADTVMEAVEFVLQNFTEM 186
           LKDLVEMCR VQHP+RGLFLR+YLAQV+RD L  I S+ EGD D  M A+EFVLQNFTEM
Sbjct: 124 LKDLVEMCRAVQHPLRGLFLRSYLAQVTRDKLPSIGSDLEGDGDAHMNALEFVLQNFTEM 183

Query: 187 NKLWVRIQLQGPAHLREKQEKERTELRDLVGKNLHVLSQIEGVDLEMYKQSVLPRVLEQV 246
           NKLWVR+Q QGP+  +EK+EKER ELRDLVGKNLHVLSQ+EGVDL +Y+ +VLPR+LEQV
Sbjct: 184 NKLWVRMQHQGPSREKEKREKERNELRDLVGKNLHVLSQLEGVDLGIYRDTVLPRILEQV 243

Query: 247 VNCKDELAQYYLMDCIIQVFPDEYHLQTLETLLGVCPQLQ-------------------- 306
           VNCKDELAQ YLMDCIIQVFPD++HLQTL+ LLG CPQLQ                    
Sbjct: 244 VNCKDELAQCYLMDCIIQVFPDDFHLQTLDVLLGACPQLQPSVDIKTVLSGLMERLSNYA 303

Query: 307 -------------------------VIEAQVDMPIVGAITLYVSLLTFTLRVHPDRLDYV 366
                                    V+EAQ D+P   ++TLY+ LL FTL V+ DRLDYV
Sbjct: 304 ASSVEALPNFLQVEAFSKLNYAIGKVVEAQADLPAAASVTLYLFLLKFTLHVYSDRLDYV 363

Query: 367 DQILGACVKTLSSQPKLEDIRATKQIVILLSAPLEKYNDIVTALTLSNYPRVMDHLDNAT 426
           DQ+LG+CV  LS+  KL D +A KQIV  LSAPLEKYN++VT L L+NYP VM++LD  T
Sbjct: 364 DQVLGSCVTQLSATGKLCDDKAAKQIVAFLSAPLEKYNNVVTILKLTNYPLVMEYLDRET 423

Query: 427 NKVMAMVIIQSIMNSNSCISTADKVDVLFELIKGLIKDLEEIIVDELDEEDFKEEQNSVA 486
           NK MA++++QS+  +N+ I+TAD+VD LFEL KGL+KD +  I DE+DEEDF+EEQN VA
Sbjct: 424 NKAMAIILVQSVFKNNTHIATADEVDALFELAKGLMKDFDGTIDDEIDEEDFQEEQNLVA 483

Query: 487 RLLHMLHNDDPLEMLKIICAVKKHILSGGPSRLPFTVPPLIFSALSLIRRLQNQDRDVVG 546
           RL++ L+ DDP EM KII  V+KHI++GGP RLP T+PPL+FSAL LIRRL+  D +  G
Sbjct: 484 RLVNKLYIDDPEEMSKIIFTVRKHIVAGGPKRLPLTIPPLVFSALKLIRRLRGGDENPFG 543

Query: 547 EEQSEAGDVVEEMHATPKKIFQLLNQLIEALSSVPAPELALRLYLECAEAANDCDLEPVA 606
           ++ S          ATPK+I QLL++ +E LS V AP+LALRLYL+CA+AAN+C+LE VA
Sbjct: 544 DDAS----------ATPKRILQLLSETVEVLSDVSAPDLALRLYLQCAQAANNCELETVA 603

Query: 607 YEFFTQAFIIYEEDIADSKAQVTAIHLIIGTLQKMNVFGIENRDTLTHKATGYSAKLLKK 666
           YEFFT+A+++YEE+I+DSKAQVTA+ LIIGTLQ+M VF +ENRDTLTHKATGYSA+LL+K
Sbjct: 604 YEFFTKAYLLYEEEISDSKAQVTALRLIIGTLQRMRVFNVENRDTLTHKATGYSARLLRK 663

Query: 667 PDQCKAVYACSHLFWVDDPEGIKDGERVLLCLKRALRIANAAQQMANVTRG--SGGPITL 726
           PDQC+AVY C+HLFW D+ E +KDGERV+LCLKRA RIA+A QQMAN +RG  S G ++L
Sbjct: 664 PDQCRAVYECAHLFWADECENLKDGERVVLCLKRAQRIADAVQQMANASRGTSSTGSVSL 723

Query: 727 FVEILNKYLHYFEKGNQQITNAAIQGLIELIRTELLTDTPSANPPSDAFFTSTLRYIQFQ 756
           +VE+LNKYL++ EKGNQQ+T   I+ L ELI++E    T      ++ F  STLRYI+FQ
Sbjct: 724 YVELLNKYLYFLEKGNQQVTGDTIKSLAELIKSE----TKKVESGAEPFINSTLRYIEFQ 783