Cp4.1LG02g05630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g05630
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionthiamine-repressible mitochondrial transport protein THI74-like isoform X3
LocationCp4.1LG02: 1652788 .. 1659263 (+)
RNA-Seq ExpressionCp4.1LG02g05630
SyntenyCp4.1LG02g05630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTGTAGTGTTCTTTAAAGCGGCATTGAAGACGTTGGGTGGTGGCAGAGTGTTAAAAGTCACACGGCTTTGAAGAGACATGTTTTGGCCTTCAAGCCAAGCATTTACTCTGGCTCTTTAAAATATGAAAAATGAAAAGAAATAAAATAAAATGGCCCATGCAGGTTTCGAACCTGCGACCTTCGCGTTATTAGCACGACGCTCTAACCAACTGAGCTAATAGGCCAGTTGAAACGTGGAGCACTATTATTTTTATTTCGTTTTCAATTTGGACTTTTTCAATTTCATTTTATGTTTCTTTTTCCGAACTTATTTATTTTTCCGCTATAACCATTTACTTATTAATTTATTTTAGAGATGTTAACTATAAATTATGTAGTATCATTTGAAAGAAGGCAAATACACTATCCATTGGCGCGTAGGGAGCTTCAAATTGAGAGAGCGCGAAAATCCTAGCAGAGAGCGTGTGTGTGGATTGATCATCTTCATCAGCATCATCAAGCGGCCGAGGGATTAACGAACTTGTTCCCCACGGCGACTCATGATCTCTCGAGGATTCAGTACAGGCTCGAGGAGGAGTTTCAGCAAAGCTATCCTGACAGCGTAAGAGCAACACGATTTTCTTCTCTCTTCTTCTTCTCGTTTATTGAATCGAGACATTAATTGGATTAAGGAGGAATTTCTCTTCACTGTTGGCATTTCACTGTACTAGTGCCAGATTCAGAGTTTGATGTAGCATTATTTCATTCTACATCGTTAAGAGCATCATAAGCTCTCTCTCGATCGATCCGAGGCACTATTTGGTTTGAGGATTAAGTTAAAATCTGGAGCTGAATTTGAACTACTTCATTCTCTCGTTTTTGCTGTTATTTCACTGTACTAATACAAGATCACGAGCGTAATGTAGAATCATTCAATTCGACAGCGTAGGAGCAAGTAATCAATCCTGACAGCGTAAGCGTTTTTCTTCTTCTTCTGAGTGGAGGTAAGATTTGTTTTGAGGAGGAAACACAAAATAATATTGAGAAAAGTTATTTCATTATCTTTTGGCTATTAGCATTTCACGCTAGTGTCGCAAGATCTTGATCATAACGTAGAACTACTTCACTCAACAGCATAAGAACAAATAATCTGTCCTGACAGAGTTAGTGCAACATAGTTCATTTTTTAGGGGAGGCACTATTTGGTTTGAGTAGGAAGTACTAGATCTTGAGCTTAATGGAGAGTTATTTCATACTTCTATTTGAGCTTATTGCTGAATCGTCCCCTTCTCTCGTCTGTCGGCATATTTCGCTGTACTAATCAACAATTTGTTAGAGTTCTTAACTTTAAATTGTTCATTTCGCAGTATAGATATTGTTGTCATGAGAATGGACTAGATAATTTTCAGGTTGAAGTTTTCGGAAATTTGATCATCCTGCAATTTCTCTTTATTTTCGAAATTCTGATTCTAGTGAGCATTTTGATTTTTTATAGAACATTTGATTAAAATTTTCCATGAAAATGGATTCAAACAATGATGATAACAGAGATTGTGAGTCTCTGTTTGACGTTTGAGATTAAAAATCACCTTAGAACACTACTGAAATTTTTCTCCGTACCTTATCTGATAAACGTATTATTGGTAATTGTTAGAAATTAGAAATATTTTTTAAACATTTTTATTAATTTATCCTAAAATTTATTTTCCCTTTTGCGCGCACACGCGTGCCATATTTGTCGCTCTGTCACAAAACCCACTGAACTCTCAACAAACTGTCATCTTCACAAAGCATTTCCGAAAAACCAGACTACATTCATTATACTGATGCCAAATCCAAAACCTTCTAAATTCCAATGCACTTTCTCGGCTAACAGCAGCTCGTCTCTGAGCACCTTTCTCAATTGACGAGCCAATTTCTCTGTCGATCCAATTACCGCCAAGAAAAACAAACAAAAAACAACCTCCTCAAATTAACACCTGTTTCTCGAGGGCAAAGAGGCTAGCTCTCATGATGTTTATCAATTCTTAGCCGAAATCGAGCTTGCCGGTTCGAATTTGAGCGGGGTTGTCGTTGGTCGGAAAATGGGCTTGAGGTATAAGTTTGGTTTGGGATTGATTTGTATGGCAGTTCTTATATGGGTTGCTTCCGCGGAAATTACTCAGGTTTGTTCTGCTTTCTTTGGATTTGTTAAGGATTTATTGTTGTTTTTGTTTGGGATTGATTGTTTCGTTAATTCGGTTTAAGAACGTTAATCACGTCGAATATTGGATTCTCTTTAACATGCATGGACAGAACAGTGGGGTGAATGTTTGTCTGTTACATGTTTGGGAAAATTATGAACCAACATGATGATTTTGACGTGGATGCCATTGTTGTTCAACTATGTTATTTGATTGTTCTTTTTGCTTTGCCTTGTGTTCGTTTAGACATTTGCCATGCTCTTTTGCTTTTAATTTTAAATATTTTGTATGTGTTGAAGCAAGTTGAAATTTGATTTTCGAATTCTTGTGAACTATCAATAAAATAGGTTGGAACTGCTTTAGTACTATGTTAATTCACTACTAAACTCGACATATCGAGTGAAATAAGGGGTTCAAATTGCTCTAATGTTGTGTTAAATTACTGTTATTAAATTTAAGCTTTAGCTAATAGTTATGGTATATTTATTCTTTTATGTATATTCTTAACCTTTCTATTGACTTGTAGCTTGGGTATTAATTTGATTGAGAGATATGCGAAATCTTAATATGAATTGTAGAGGTTTAAGTTACTGTAATATCATGTTAAATCACCAATAAATTTGCTGAAGTGTTTTTAAGTGAAACGCTTATTATATAAGCACTTTTCGAAGAAAATGTGTTAAACGCTTCATCTTCGAACACTGCACATTTTTAAGAAAGACAGTCACAAACTTATGCTTTGTGTACATTCTTAGAATTTGGGAAAACTACAACTTACCCAATCCTTGTTTGTGCAGAGAATCTTCACTGAATATAAACAGCCTTTTGCACTCTCTTATTTAGGGGTGTCTCTCATGGTGGTATATTTACCAGTGGCAGTTGTCAAAGATTTGGTTTGCAGCTTATTAAACCCAGATCTACTCAATGACCACCATGAAAATGATGGCTCTGTTCCAAGCTCTTCAATTGGACTTGATGTTCCTTTGAAATTTAATGAAATACATTATAAATTTGATGAAGACTTGAAAAGCTGCCTAACTAGTGACAAAGATTTGAGTGAAAGGGAAGAGGGACAGCCTTTGATGTCTAATTTTGAATCACATCATAAAGTTAGTACACGGGAAATCATTGAACGCAGCCTTTATCTTACTCCCATATGGTTCACTACTGAGGTACACAGTTCTAAACTGTTTTTGTTTGTTGACTTTGCTGATTTTGTTGTTTAACGTTGTCTACTTCATGTTCTTTTAGCTGATTATAAACGTGGTTGATATTTGTAGCTTAACGTTCATTTGTGATTAATAACTCTAGTTAAACAACACTTCTGGTTTCTGAACTTTTGTGAAAATAACAATTTAGTCTATGAATTGTCAAATTGGTGACATTTAATTTTTGAACTTTAGTATGTACTATTTAGTCCATGCACTTTAAAAATTGTATTAATTTAATCCGTACTGTGAAAAATCCATGAAAAGAATTAAGAGTTTAACGACTTAGTTTTCTAGTTTTAACACATTTGATCTCTAATAAGTTTGGTAAGATATTCCATTAAATCTTAACAATAGTTTTCACAATAGAGACTAAATCCTTACAAAATCATAGTATAAATTGTTACATAGTAAAGTACAAAAATTTAATTGTTACAATTAGAGTTCAAGGAAGGACTAAATTATTAGATTACATGTTTTTTTTAACAAAAAAAAAAATGGTCCTATTTGATAACGTTTTGGTTTTGAGGTTCAGGCTGTTCTAATCCTCGCTCAAAAACATTTTTGAGATCATTGCTAGAATTTGAAAAGTGATATAAAGTTATTGTTTTCTGATTATAAAATTGGACCAAGATTTTAATAACGTTTTCAAAAAGTGAAAAAGTAACCAAAGAAAAAAAAAAGAGGCACTGTATAAATATCTATTGAGTTCATCGGTTATCATTGATTGTTAACTTTAAGATCTCATTTATCTGCAATGTCACTTTGATAAATTTGTTTGAGACTTTCACAGTACTTCTCGAACTCGGCCCTGGCAAACACGAGCGTTGCTACGGCAACCATCTTAAATTCCACATCAGGCCTTTTTGCCCTGCTGTTTGGAGCTCTTGTTGGACAAGAATCCATAACCATTACCAAGGTGGTTGCTGTTTTCATTAGTATGGCTGGAGTTGTCATGACAACCCTGGGAAAAACTTGGGCAACCAATGGGTTCCCAGTCATCTCTGAGTAAGTTTTGCCATTGATTACCATAGTTTAAATAATCTTCATTTCATATTTAAGATGAAGCAATCGCATATGACTTTTCTTTTGGCCAAGAGCCCAGCCCTGAGTCATATATATAATACGCACATGCACGTGCACACACACACGCACGATGTATTGGGCCTCAGCCACTACCCACGAGGAGACTTCGATGAGATTTTCATCAAATAATGTTTGTCTAAAGTTCCTACTTTCTGGTGTGACATATTTTTATTTGCTTATTTCTATTTTATTTAATTGATATGTCGAGCTCACTAAAATTTTCGAGCCAATAAAAATGTCACATTAGAAAGTAAACAGGAGCTTCTTCTTACCACTAGTAAGTAGGCAAGTATGGTTCATTTTCATTATCGAGCTTTAGTTACACTTGTGAGGAACTATCCCTGAACATTTAATCCCTTATTGTCTGACCCATGCCTTGAGCTCTAGTATCTTACATTTTACATTGTTTCCAGATAACCAGCTGTTGATGGTAAAGTCCACCTTAAAATGAAAAAACGTCCACATGTACTAAATTCTTTCTTGTAACTTACTGTCTTTTCTTCCTTTATTGGTGAGGTCTCGAGGGAGAACCATCACCGGGGACGTTTTTTGTCTTCTCTCCGCAGCAATCTATGGATTATTTACTGGTATGGCATTCTAAACCTAACTAGTAACTGTTTTTGTTGAGGATTCAAATGTTTTACTTCATGGTAATTATTGAATTGTGTGATTGGTATAGTGCTGCTCAAGAAATTAGCTGGATCAGGAGGAGACAAGATTGATGTGCAGAAGTTCTTTGGATATGTTGGTCTATTTACCATAGTGGGTCTTTGGTGGCTTGGTAAGTAGTGTTTGGTGAATGTTTATCAAGTCAATTTGATGATTTGGTTCTGTTTAAGTTTCGTTGTGCCTTTTCCATTAAATGGTCATGTGAAATTGTAAATGATAGAATTAACCAAGACAATGTTGTTGCTGCCTTTAACTGCATTGTTCTTACTATTAAAGTCACTCTGTAGTTTGGCCTCTAACTGCTATGGGCATTGAACCTCCTTTCAAGTTTCCTCCCTCAAAATCCATCACTGAAATCGTGGTTTTGAATGGCTTCGTTGGGAACGTTCTATCCGATTACTTTTGGTATGTCGAGAATACTATATATTTTTTGGGTTGACCACATAGCCCTCTTCATGACAACTGAATGAAATTTTGCTCTCTTTATTCCCCAAAATATTCATTTTACACTTGTTGTTGATGCTATGTGATTGTCTTGAATTACATACATGGAGAAACTGTTTTGCCCTTGCAGGGCCCTCTCTGTGATTTGGACTTCTCCATTAGTTTCTAACCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCCGACGTGATATTGCACGGTCGTCGCTACTCCACCCTCTACATTGTAGGTTGTGTTCAGGTAAACGCTCATCCGCTCAACAAAATTTGAGCATGATATCTATTTAGTCGGTATTTTGGATATGTTTAGTTCGCATCTGAATGTCATTCATAGGTTAAGGAATCTGTTACTATCCCAAAGCTCGATGGTTTGATCTCCACCCCAATATTCATATATCATGCCATCTTATCTAAATCCTAGGATATCGTATTTGTACTCGAGCATTCGATACAGAGGCGGGCAGGACGGGAAGTTCTTAGCTCTATGTTCTTCTGCTTTCTATTCAGGTATTTGCTGGATTTTTCATAGTGAATTTTTCAGACAAGATCTCTGCTGGAGGCAGCCAGTGCCTTTGAAGAAGAGGAGGGTGTACATAGAGATTTGGTATAAACTGGTGGAAACTACTTTGAAGCATGAGACGTATGAGTATATAATCCTGGGTTTTAGAGGGAAATTGTAAAATATTTCAAGATTTATAGGGGAAAATAACATTAAATTTAGTTGTTATAATCATATGTGTTAGATTTAGAGACTAGTTTCTTTTTTCTTAATTTTTTTCTTCTAATTTTCTAATTTTTTGTATTTTATTTTACTTTTTTTATTAGTTTTTGTTTGAAAATCATTAAATAATATTATAGCTAAGTTAAATTTTTATTT

mRNA sequence

ATGAATTGCAAATACACTATCCATTGGCGCGTAGGGAGCTTCAAATTGAGAGAGCGCGAAAATCCTAGCAGAGAGCGTCATCATCAAGCGGCCGAGGGATTAACGAACTTGTTCCCCACGGCGACTCATGATCTCTCGAGGATTCAGTACAGGCTCGAGGAGGAGTTTCAGCAAAGCTATCCTGACAGCGAGCAAGTAATCAATCCTGACAGCGTAAGCGTTTTTCTTCTTCTTCTGAGTGGAGCCGAAATCGAGCTTGCCGGTTCGAATTTGAGCGGGGTTGTCGTTGGTCGGAAAATGGGCTTGAGGTATAAGTTTGGTTTGGGATTGATTTGTATGGCAGTTCTTATATGGGTTGCTTCCGCGGAAATTACTCAGAGAATCTTCACTGAATATAAACAGCCTTTTGCACTCTCTTATTTAGGGGTGTCTCTCATGGTGGTATATTTACCAGTGGCAGTTGTCAAAGATTTGGTTTGCAGCTTATTAAACCCAGATCTACTCAATGACCACCATGAAAATGATGGCTCTGTTCCAAGCTCTTCAATTGGACTTGATGTTCCTTTGAAATTTAATGAAATACATTATAAATTTGATGAAGACTTGAAAAGCTGCCTAACTAGTGACAAAGATTTGAGTGAAAGGGAAGAGGGACAGCCTTTGATGTCTAATTTTGAATCACATCATAAAGTTAGTACACGGGAAATCATTGAACGCAGCCTTTATCTTACTCCCATATGGTTCACTACTGAGTACTTCTCGAACTCGGCCCTGGCAAACACGAGCGTTGCTACGGCAACCATCTTAAATTCCACATCAGGCCTTTTTGCCCTGCTGTTTGGAGCTCTTGTTGGACAAGAATCCATAACCATTACCAAGGTGGTTGCTGTTTTCATTAGTATGGCTGGAGTTGTCATGACAACCCTGGGAAAAACTTGGGCAACCAATGGGTTCCCAGTCATCTCTGAGTCTCGAGGGAGAACCATCACCGGGGACGTTTTTTGTCTTCTCTCCGCAGCAATCTATGGATTATTTACTGTGCTGCTCAAGAAATTAGCTGGATCAGGAGGAGACAAGATTGATGTGCAGAAGTTCTTTGGATATGTTGGTCTATTTACCATAGTGGGTCTTTGGTGGCTTGTTTGGCCTCTAACTGCTATGGGCATTGAACCTCCTTTCAAGTTTCCTCCCTCAAAATCCATCACTGAAATCGTGGTTTTGAATGGCTTCGTTGGGAACGTTCTATCCGATTACTTTTGGGCCCTCTCTGTGATTTGGACTTCTCCATTAGTTTCTAACCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCCGACGTGATATTGCACGGTCGTCGCTACTCCACCCTCTACATTGTAGGTTGTGTTCAGGTATTTGCTGGATTTTTCATAGTGAATTTTTCAGACAAGATCTCTGCTGGAGGCAGCCAGTGCCTTTGAAGAAGAGGAGGGTGTACATAGAGATTTGGTATAAACTGGTGGAAACTACTTTGAAGCATGAGACGTATGAGTATATAATCCTGGGTTTTAGAGGGAAATTGTAAAATATTTCAAGATTTATAGGGGAAAATAACATTAAATTTAGTTGTTATAATCATATGTGTTAGATTTAGAGACTAGTTTCTTTTTTCTTAATTTTTTTCTTCTAATTTTCTAATTTTTTGTATTTTATTTTACTTTTTTTATTAGTTTTTGTTTGAAAATCATTAAATAATATTATAGCTAAGTTAAATTTTTATTT

Coding sequence (CDS)

ATGAATTGCAAATACACTATCCATTGGCGCGTAGGGAGCTTCAAATTGAGAGAGCGCGAAAATCCTAGCAGAGAGCGTCATCATCAAGCGGCCGAGGGATTAACGAACTTGTTCCCCACGGCGACTCATGATCTCTCGAGGATTCAGTACAGGCTCGAGGAGGAGTTTCAGCAAAGCTATCCTGACAGCGAGCAAGTAATCAATCCTGACAGCGTAAGCGTTTTTCTTCTTCTTCTGAGTGGAGCCGAAATCGAGCTTGCCGGTTCGAATTTGAGCGGGGTTGTCGTTGGTCGGAAAATGGGCTTGAGGTATAAGTTTGGTTTGGGATTGATTTGTATGGCAGTTCTTATATGGGTTGCTTCCGCGGAAATTACTCAGAGAATCTTCACTGAATATAAACAGCCTTTTGCACTCTCTTATTTAGGGGTGTCTCTCATGGTGGTATATTTACCAGTGGCAGTTGTCAAAGATTTGGTTTGCAGCTTATTAAACCCAGATCTACTCAATGACCACCATGAAAATGATGGCTCTGTTCCAAGCTCTTCAATTGGACTTGATGTTCCTTTGAAATTTAATGAAATACATTATAAATTTGATGAAGACTTGAAAAGCTGCCTAACTAGTGACAAAGATTTGAGTGAAAGGGAAGAGGGACAGCCTTTGATGTCTAATTTTGAATCACATCATAAAGTTAGTACACGGGAAATCATTGAACGCAGCCTTTATCTTACTCCCATATGGTTCACTACTGAGTACTTCTCGAACTCGGCCCTGGCAAACACGAGCGTTGCTACGGCAACCATCTTAAATTCCACATCAGGCCTTTTTGCCCTGCTGTTTGGAGCTCTTGTTGGACAAGAATCCATAACCATTACCAAGGTGGTTGCTGTTTTCATTAGTATGGCTGGAGTTGTCATGACAACCCTGGGAAAAACTTGGGCAACCAATGGGTTCCCAGTCATCTCTGAGTCTCGAGGGAGAACCATCACCGGGGACGTTTTTTGTCTTCTCTCCGCAGCAATCTATGGATTATTTACTGTGCTGCTCAAGAAATTAGCTGGATCAGGAGGAGACAAGATTGATGTGCAGAAGTTCTTTGGATATGTTGGTCTATTTACCATAGTGGGTCTTTGGTGGCTTGTTTGGCCTCTAACTGCTATGGGCATTGAACCTCCTTTCAAGTTTCCTCCCTCAAAATCCATCACTGAAATCGTGGTTTTGAATGGCTTCGTTGGGAACGTTCTATCCGATTACTTTTGGGCCCTCTCTGTGATTTGGACTTCTCCATTAGTTTCTAACCTGGGCATGTCATTGACAATTCCCCTTGCTATGCTGGCCGACGTGATATTGCACGGTCGTCGCTACTCCACCCTCTACATTGTAGGTTGTGTTCAGGTATTTGCTGGATTTTTCATAGTGAATTTTTCAGACAAGATCTCTGCTGGAGGCAGCCAGTGCCTTTGA

Protein sequence

MNCKYTIHWRVGSFKLRERENPSRERHHQAAEGLTNLFPTATHDLSRIQYRLEEEFQQSYPDSEQVINPDSVSVFLLLLSGAEIELAGSNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLVCSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQPLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL
Homology
BLAST of Cp4.1LG02g05630 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.7e-37
Identity = 128/427 (29.98%), Postives = 208/427 (48.71%), Query Frame = 0

Query: 89  SNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVV 148
           S  SG    R+M L    G+ ++ +  +IWVAS+E+T  +FT+Y +PF  ++   S+ V+
Sbjct: 58  SQNSGFTQRRRMAL----GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVL 117

Query: 149 YLPVAVVKDLVCSLLNPDLLNDHH-------------ENDGSVPSS-SIGLDVPLKFNEI 208
           YL   ++           L   H                D ++ SS S  L VP+KF+++
Sbjct: 118 YLLGFIIWKPWRQQCTRGLRGKHAAFFADAEGYFAACATDTTMNSSLSEPLYVPVKFHDL 177

Query: 209 HYKFDEDL-----KSCLTSDKDLSEREEGQPLMSNFESHHKVS----------------- 268
             +  E       K+   S    S   E + L SN     K+S                 
Sbjct: 178 PSEKPESTNIDTEKTPKKSRVRFSNIMEIRQLPSNHALESKLSRMSYPVKEQESILKTVG 237

Query: 269 ---TREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES- 328
                ++ + S +   +WF        AL++T VA   IL+STSGLF L+  A+    S 
Sbjct: 238 KLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSG 297

Query: 329 --ITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLFT 388
              T++K++AV +S+ GVV+  L  +          +S GR   G ++ L  A +Y ++ 
Sbjct: 298 DRFTLSKLLAVILSIGGVVLVNLSGS---------EKSAGRDTIGSIWSLAGAMLYAVYI 357

Query: 389 VLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVV 448
           V++K+      DK+D+  FFG+VGLF ++ LW   + L   G E  F+F P+K +   ++
Sbjct: 358 VMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLMCII 417

Query: 449 LNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQV 474
           +NG +G VLS++ W      TS L+  L +SLTIPL+++AD+ +   ++S L+  G + V
Sbjct: 418 INGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPV 468

BLAST of Cp4.1LG02g05630 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 2.9e-37
Identity = 114/389 (29.31%), Postives = 194/389 (49.87%), Query Frame = 0

Query: 103 RYKFGLGLICMAVLIWVASAEITQRIFTE--YKQPFALSYLGVSLMVVYLPVAVVKDLVC 162
           R+  GL ++ + +++WV S+ +   IF +  Y++PF ++Y   +  + YL     K +V 
Sbjct: 12  RWTLGLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYL-FPTAKAVVV 71

Query: 163 SLLNPDLLNDH------HENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSE 222
           +  +    N H       E  GS  + S+ +  PL                      L+ 
Sbjct: 72  NYKDTGRANVHRELIMEEEGTGSDSNRSVDMTSPL----------------------LTN 131

Query: 223 REEGQPLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSG 282
            E G    ++     +++  E I+ S     +WFT    +N++LA TSVA+ TIL++TS 
Sbjct: 132 LEAG----THANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILSTTSS 191

Query: 283 LFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRG-----RTI 342
            F L  GA+   ES++ +KV+  FIS  G++M T   +       +   S       + +
Sbjct: 192 FFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHIADVSGDDNDAVQVL 251

Query: 343 TGDVFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGI 402
            G++  L  A +YG+++ LLK+  G    +++++ FFG+VGLF ++ LW  +  L   G 
Sbjct: 252 IGNLLALAGAVLYGVYSTLLKREVGD-ETRVNMKIFFGFVGLFNLLFLWPSLIVLDFFGW 311

Query: 403 EPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVI 462
           E PF  P    +  I+ +N  +   +SD+ WA +++ TSPL   +G+S+TIPLAM  DVI
Sbjct: 312 E-PFSLPKDPKVVVIIFVNCLI-TFVSDFCWAKAMLLTSPLTVTVGLSITIPLAMFGDVI 370

Query: 463 LHGRRYSTLYIVGCVQVFAGFFIVNFSDK 479
              +  S LY+ G   +   FFI+N S +
Sbjct: 372 FKHKTMSALYLFGATLILGSFFIINKSSE 370

BLAST of Cp4.1LG02g05630 vs. ExPASy Swiss-Prot
Match: Q8R314 (Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 8.4e-37
Identity = 123/428 (28.74%), Postives = 209/428 (48.83%), Query Frame = 0

Query: 89  SNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVV 148
           S  SG    R+M L    G+ ++ +  +IWVAS+E+T  +FT+Y +PF  ++   S+ V+
Sbjct: 58  SQSSGFSQRRRMAL----GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVL 117

Query: 149 YL--------------------PVAVVKDL------------VCSLLNPDL---LNDHHE 208
           YL                    P A   D             + S L+  L   +  H  
Sbjct: 118 YLLGFIIWKPWRQQCTRGFRGKPAAFFADAEGYFAACTTDTSMSSSLSEPLYVPVKFHDL 177

Query: 209 NDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDK-DLSEREEGQPLMSNFES----H 268
               + S++IG +   K + + +    +++   +S   +        P + + ES     
Sbjct: 178 PSEKLESTNIGTEKTPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKDQESILKTV 237

Query: 269 HKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES 328
            K++  ++ + S +   +WF        AL++T VA   IL+STSGLF L+  A+    S
Sbjct: 238 GKLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNS 297

Query: 329 ---ITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLF 388
               T++K++AV +S+ GVV+  L  +          +S GR   G ++ L  A  Y ++
Sbjct: 298 GDRFTLSKLLAVILSIGGVVLVNLSGS---------EKSAGRDTIGSIWSLAGAMFYAVY 357

Query: 389 TVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIV 448
            V++K+      DK+D+  FFG+VGLF ++ LW   + L   G E  F+F P+K +   +
Sbjct: 358 IVMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLLCI 417

Query: 449 VLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQ 474
           ++NG +G VLS++ W      TS L+  L +SLTIPL+++AD+ +   ++S L+  G + 
Sbjct: 418 IINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIP 469

BLAST of Cp4.1LG02g05630 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.4e-36
Identity = 125/427 (29.27%), Postives = 210/427 (49.18%), Query Frame = 0

Query: 89  SNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVV 148
           S  SG    ++M L    G+ ++ +  +IWVAS+E+T  +FT+Y +PF  ++   S+ V+
Sbjct: 58  SQSSGFTQRKRMAL----GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVL 117

Query: 149 YLPVAVVKDLVCSLLNPDLLNDHH-------------ENDGSVPSS-SIGLDVPLKFNEI 208
           YL   +V               H                D ++ SS S  L VP+KF+++
Sbjct: 118 YLLGFIVWKPWRQQCTRGFRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDL 177

Query: 209 HYKFDEDL---------KSCLTSDKDLSERE-------------EGQPLMSNFES----H 268
             +  E+          KS +     +  R+                P +   ES     
Sbjct: 178 PSEKPENTNIDTEKIPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQESLLKTV 237

Query: 269 HKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES 328
            K++  ++ + S +   +WF   +    AL++T VA   IL+STSGLF L+  A+    S
Sbjct: 238 GKLTATQVAKISFFFCFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNS 297

Query: 329 ---ITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLF 388
               T++K++AV +S+ GVV+  L  +          +S GR   G ++ L+ A +Y ++
Sbjct: 298 GDRFTLSKLLAVILSIGGVVLVNLSGS---------EKSPGRNTIGSIWSLVGAMLYAVY 357

Query: 389 TVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIV 448
            V++K+      DK+D+  FFG+VGLF ++ LW   + L   G E  F+F P+K +   +
Sbjct: 358 IVMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLMCI 417

Query: 449 VLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQ 473
           V+NG +G VLS++ W      TS L+  L +SLTIPL+++AD+ +   ++S L+  G + 
Sbjct: 418 VINGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIP 468

BLAST of Cp4.1LG02g05630 vs. ExPASy Swiss-Prot
Match: Q8WV83 (Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 2.4e-36
Identity = 125/427 (29.27%), Postives = 209/427 (48.95%), Query Frame = 0

Query: 89  SNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVV 148
           S  SG    R+M L    G+ ++ +  +IWVAS+E+T  +FT+Y +PF  ++   S+ V+
Sbjct: 58  SQNSGFTQRRRMAL----GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVL 117

Query: 149 YLPVAVVKDLVCSLLNPDLLNDHH-------------ENDGSVPSS-SIGLDVPLKFNEI 208
           YL   ++           L   H                D ++ SS S  L VP+KF+++
Sbjct: 118 YLLGFIIWKPWRQQCTRGLRGKHAAFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDL 177

Query: 209 HYKFDEDL-----KSCLTSDKDLSEREEGQPLMSN--------------------FESHH 268
             +  E       K+   S    S   E + L S+                     ++  
Sbjct: 178 PSEKPESTNIDTEKTPKKSRVRFSNIMEIRQLPSSHALEAKLSRMSYPVKEQESILKTVG 237

Query: 269 KVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQES- 328
           K++  ++ + S +   +WF        AL++T VA   IL+STSGLF L+  A+    S 
Sbjct: 238 KLTATQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSG 297

Query: 329 --ITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLFT 388
              T++K++AV +S+ GVV+  L  +          +  GR   G ++ L  A +Y ++ 
Sbjct: 298 DRFTLSKLLAVILSIGGVVLVNLAGS---------EKPAGRDTVGSIWSLAGAMLYAVYI 357

Query: 389 VLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVV 448
           V++K+      DK+D+  FFG+VGLF ++ LW   + L   G E  F+F P+K +   ++
Sbjct: 358 VMIKRKV-DREDKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEF-PNKVVLMCII 417

Query: 449 LNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQV 474
           +NG +G VLS++ W      TS L+  L +SLTIPL+++AD+ +   ++S L+  G + V
Sbjct: 418 INGLIGTVLSEFLWLWGCFLTSSLIGTLALSLTIPLSIIADMCMQKVQFSWLFFAGAIPV 468

BLAST of Cp4.1LG02g05630 vs. NCBI nr
Match: KAG7037663.1 (hypothetical protein SDJN02_01293 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 805 bits (2078), Expect = 7.62e-292
Identity = 415/440 (94.32%), Postives = 421/440 (95.68%), Query Frame = 0

Query: 48  IQYRLEEEFQQSYPDSEQVINPDSVSVFLLLLSGAEIELAGSNLSGVVVGRKMGLRYKFG 107
           IQYRLEEEFQQSYPDS +  + D V  FL     AEIEL+GSNLSGVVVGR+MGLRYKFG
Sbjct: 2   IQYRLEEEFQQSYPDSVKEASSDDVYQFL-----AEIELSGSNLSGVVVGREMGLRYKFG 61

Query: 108 LGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLVCSLLNPDL 167
           LGLI MAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV SLLNPDL
Sbjct: 62  LGLIGMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLVYSLLNPDL 121

Query: 168 LNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQPLMSNFES 227
           LNDHHENDGSVPSSSIGL VPLKFNEIHY FDEDLKSCLTSDKDLSE EEGQ LMSNFES
Sbjct: 122 LNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSCLTSDKDLSEEEEGQLLMSNFES 181

Query: 228 HHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQE 287
           HHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQE
Sbjct: 182 HHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQE 241

Query: 288 SITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSAAIYGLFTV 347
           SITITKVVAVFISMAGVVMTTLGKTWATNGFPVI ESRGRTITGDVFCLLSAAIYGLFTV
Sbjct: 242 SITITKVVAVFISMAGVVMTTLGKTWATNGFPVIIESRGRTITGDVFCLLSAAIYGLFTV 301

Query: 348 LLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVVL 407
           LLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVVL
Sbjct: 302 LLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSKSITEIVVL 361

Query: 408 NGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQVF 467
           NGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADV+LHGRRYSTLYI+GCVQVF
Sbjct: 362 NGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVVLHGRRYSTLYIIGCVQVF 421

Query: 468 AGFFIVNFSDKISAGGSQCL 487
           AGFFIVNFSDKISAGGSQCL
Sbjct: 422 AGFFIVNFSDKISAGGSQCL 436

BLAST of Cp4.1LG02g05630 vs. NCBI nr
Match: XP_023524239.1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 766 bits (1977), Expect = 3.01e-277
Identity = 388/388 (100.00%), Postives = 388/388 (100.00%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
           CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ
Sbjct: 61  CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 360

Query: 460 IVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           IVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 361 IVGCVQVFAGFFIVNFSDKISAGGSQCL 388

BLAST of Cp4.1LG02g05630 vs. NCBI nr
Match: XP_022982173.1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X4 [Cucurbita maxima])

HSP 1 Score: 749 bits (1935), Expect = 7.52e-271
Identity = 379/388 (97.68%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHY FDE LKSCLTSDKDLSEREEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCLTSDKDLSEREEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVST EIIERSLYLTP+WFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGD+FCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITE VVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYSTLY
Sbjct: 301 SITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADIVLHGRRYSTLY 360

Query: 460 IVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           IVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 361 IVGCVQVFAGFFIVNFSDKISAGGSQCL 388

BLAST of Cp4.1LG02g05630 vs. NCBI nr
Match: XP_022940128.1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 745 bits (1923), Expect = 5.06e-269
Identity = 378/388 (97.42%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGL VPLKFNEIHY FDEDLKS LTSDKDLSE EEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSSLTSDKDLSEEEEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGV MTTLGKTWATNGFPVI ESRGRTITGDVFCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVAMTTLGKTWATNGFPVIIESRGRTITGDVFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYST+Y
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADMVLHGRRYSTIY 360

Query: 460 IVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           IVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 361 IVGCVQVFAGFFIVNFSDKISAGGSQCL 388

BLAST of Cp4.1LG02g05630 vs. NCBI nr
Match: XP_022982168.1 (uncharacterized transporter C405.03c-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 731 bits (1888), Expect = 2.13e-263
Identity = 384/462 (83.12%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 26  RHHQAAEGLTNLFPTATHDLSRIQYRLEEEFQQSYPDSEQVINPDSVSVFLLLLSGAEIE 85
           +HHQAAEGLTNLF   THDLS IQYRLEEEFQ SYPDS                      
Sbjct: 8   QHHQAAEGLTNLFSKVTHDLSMIQYRLEEEFQLSYPDS---------------------- 67

Query: 86  LAGSNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSL 145
                                                    RIFTEYKQPFALSYLGVSL
Sbjct: 68  -----------------------------------------RIFTEYKQPFALSYLGVSL 127

Query: 146 MVVYLPVAVVKDLVCSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSC 205
           MVVYLPVAVVKDLV SLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHY FDE LKSC
Sbjct: 128 MVVYLPVAVVKDLVYSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSC 187

Query: 206 LTSDKDLSEREEGQPLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVAT 265
           LTSDKDLSEREEGQPLMSNFESHHKVST EIIERSLYLTP+WFTTEYFSNSALANTSVAT
Sbjct: 188 LTSDKDLSEREEGQPLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVAT 247

Query: 266 ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR 325
           ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR
Sbjct: 248 ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR 307

Query: 326 GRTITGDVFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT 385
           GRTITGD+FCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT
Sbjct: 308 GRTITGDIFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT 367

Query: 386 AMGIEPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML 445
           AMGIEPPFKFPPSKSITE VVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML
Sbjct: 368 AMGIEPPFKFPPSKSITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML 406

Query: 446 ADVILHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           AD++LHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 428 ADIVLHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL 406

BLAST of Cp4.1LG02g05630 vs. ExPASy TrEMBL
Match: A0A6J1J463 (thiamine-repressible mitochondrial transport protein THI74-like isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 749 bits (1935), Expect = 3.64e-271
Identity = 379/388 (97.68%), Postives = 383/388 (98.71%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHY FDE LKSCLTSDKDLSEREEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCLTSDKDLSEREEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVST EIIERSLYLTP+WFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGD+FCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITE VVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYSTLY
Sbjct: 301 SITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADIVLHGRRYSTLY 360

Query: 460 IVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           IVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 361 IVGCVQVFAGFFIVNFSDKISAGGSQCL 388

BLAST of Cp4.1LG02g05630 vs. ExPASy TrEMBL
Match: A0A6J1FNF4 (thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111445844 PE=3 SV=1)

HSP 1 Score: 745 bits (1923), Expect = 2.45e-269
Identity = 378/388 (97.42%), Postives = 381/388 (98.20%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGL VPLKFNEIHY FDEDLKS LTSDKDLSE EEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSSLTSDKDLSEEEEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGV MTTLGKTWATNGFPVI ESRGRTITGDVFCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVAMTTLGKTWATNGFPVIIESRGRTITGDVFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYST+Y
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADMVLHGRRYSTIY 360

Query: 460 IVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           IVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 361 IVGCVQVFAGFFIVNFSDKISAGGSQCL 388

BLAST of Cp4.1LG02g05630 vs. ExPASy TrEMBL
Match: A0A6J1J459 (uncharacterized transporter C405.03c-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 731 bits (1888), Expect = 1.03e-263
Identity = 384/462 (83.12%), Postives = 389/462 (84.20%), Query Frame = 0

Query: 26  RHHQAAEGLTNLFPTATHDLSRIQYRLEEEFQQSYPDSEQVINPDSVSVFLLLLSGAEIE 85
           +HHQAAEGLTNLF   THDLS IQYRLEEEFQ SYPDS                      
Sbjct: 8   QHHQAAEGLTNLFSKVTHDLSMIQYRLEEEFQLSYPDS---------------------- 67

Query: 86  LAGSNLSGVVVGRKMGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSL 145
                                                    RIFTEYKQPFALSYLGVSL
Sbjct: 68  -----------------------------------------RIFTEYKQPFALSYLGVSL 127

Query: 146 MVVYLPVAVVKDLVCSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSC 205
           MVVYLPVAVVKDLV SLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHY FDE LKSC
Sbjct: 128 MVVYLPVAVVKDLVYSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSC 187

Query: 206 LTSDKDLSEREEGQPLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVAT 265
           LTSDKDLSEREEGQPLMSNFESHHKVST EIIERSLYLTP+WFTTEYFSNSALANTSVAT
Sbjct: 188 LTSDKDLSEREEGQPLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVAT 247

Query: 266 ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR 325
           ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR
Sbjct: 248 ATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESR 307

Query: 326 GRTITGDVFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT 385
           GRTITGD+FCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT
Sbjct: 308 GRTITGDIFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLT 367

Query: 386 AMGIEPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML 445
           AMGIEPPFKFPPSKSITE VVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML
Sbjct: 368 AMGIEPPFKFPPSKSITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAML 406

Query: 446 ADVILHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL 487
           AD++LHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL
Sbjct: 428 ADIVLHGRRYSTLYIVGCVQVFAGFFIVNFSDKISAGGSQCL 406

BLAST of Cp4.1LG02g05630 vs. ExPASy TrEMBL
Match: A0A6J1IYL1 (thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481080 PE=3 SV=1)

HSP 1 Score: 706 bits (1822), Expect = 9.56e-254
Identity = 357/366 (97.54%), Postives = 361/366 (98.63%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHY FDE LKSCLTSDKDLSEREEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYNFDEGLKSCLTSDKDLSEREEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVST EIIERSLYLTP+WFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTWEIIERSLYLTPLWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGD+FCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDIFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITE VVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYSTLY
Sbjct: 301 SITESVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADIVLHGRRYSTLY 360

Query: 460 IVGCVQ 465
           IVGCVQ
Sbjct: 361 IVGCVQ 366

BLAST of Cp4.1LG02g05630 vs. ExPASy TrEMBL
Match: A0A6J1FHK5 (thiamine-repressible mitochondrial transport protein THI74-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445844 PE=3 SV=1)

HSP 1 Score: 701 bits (1810), Expect = 6.41e-252
Identity = 356/366 (97.27%), Postives = 359/366 (98.09%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV
Sbjct: 1   MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 60

Query: 160 CSLLNPDLLNDHHENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREEGQ 219
            SLLNPDLLNDHHENDGSVPSSSIGL VPLKFNEIHY FDEDLKS LTSDKDLSE EEGQ
Sbjct: 61  YSLLNPDLLNDHHENDGSVPSSSIGLGVPLKFNEIHYNFDEDLKSSLTSDKDLSEEEEGQ 120

Query: 220 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 279
           PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL
Sbjct: 121 PLMSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSVATATILNSTSGLFALL 180

Query: 280 FGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISESRGRTITGDVFCLLSA 339
           FGALVGQESITITKVVAVFISMAGV MTTLGKTWATNGFPVI ESRGRTITGDVFCLLSA
Sbjct: 181 FGALVGQESITITKVVAVFISMAGVAMTTLGKTWATNGFPVIIESRGRTITGDVFCLLSA 240

Query: 340 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 399
           AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK
Sbjct: 241 AIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPPSK 300

Query: 400 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLY 459
           SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLAD++LHGRRYST+Y
Sbjct: 301 SITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPLAMLADMVLHGRRYSTIY 360

Query: 460 IVGCVQ 465
           IVGCVQ
Sbjct: 361 IVGCVQ 366

BLAST of Cp4.1LG02g05630 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 404.4 bits (1038), Expect = 1.3e-112
Identity = 219/398 (55.03%), Postives = 281/398 (70.60%), Query Frame = 0

Query: 100 MGLRYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPVAVVKDLV 159
           MG RYK GL LI   V+IWV SAE+TQ IFT YKQPFA++YLG SLM+VYLPVA +KD +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 160 CSLLNPDLLNDHHENDGSVPS----SSIGLDVPLKFNEIHYKFDEDLKSCLT---SDKDL 219
           C       L+        +P+    SS+ L  PL+    H   +  L+  +T   S++ L
Sbjct: 61  CR-----YLDRRSSKSNKIPALTDDSSVELGSPLR----HKIIEMGLQGTITKKDSEEHL 120

Query: 220 S--EREEGQPL-------MSNFESHHKVSTREIIERSLYLTPIWFTTEYFSNSALANTSV 279
           S  E E+ +PL       +   +   +++T++I    LYL PIWF TEY SN+ALA TSV
Sbjct: 121 SSHEEEDERPLIGKVREEVQTLKQRKEITTKQIAMYGLYLAPIWFVTEYLSNAALARTSV 180

Query: 280 ATATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMTTLGKTWATNGFPVISE 339
           A+ T+L+STSGLF L  G  +GQ+++ ++KVVAVF+SMAGVVMTTLGKTWA++   + S 
Sbjct: 181 ASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVVMTTLGKTWASDESQLNSS 240

Query: 340 SRG-RTITGDVFCLLSAAIYGLFTVLLKKLAGSGGDKIDVQKFFGYVGLFTIVGLWWLVW 399
             G R++ GD+F LLSA  YGLFTVLLKK AG  G+ +DVQK FGY+GLFT+V LWWLVW
Sbjct: 241 LNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQKLFGYIGLFTLVALWWLVW 300

Query: 400 PLTAMGIEPPFKFPPSKSITEIVVLNGFVGNVLSDYFWALSVIWTSPLVSNLGMSLTIPL 459
           PLTA+GIEP F  P S  + E+V+ NGF+G+VLSDYFWALSV+WT+PLV+ LGMSLTIPL
Sbjct: 301 PLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFWALSVVWTTPLVATLGMSLTIPL 360

Query: 460 AMLADVILHGRRYSTLYIVGCVQVFAGFFIVNFSDKIS 481
           AM+AD+++HGR YS +YI+G  QVFAGF I N SD  S
Sbjct: 361 AMVADMMIHGRHYSAIYILGSTQVFAGFVIANISDLFS 389

BLAST of Cp4.1LG02g05630 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-21
Identity = 108/421 (25.65%), Postives = 183/421 (43.47%), Query Frame = 0

Query: 103 RYKFGLGLICMAVLIWVASAEITQRIFTEYKQPFALSYLGVSLMVVYLPV----AVVKDL 162
           R+  GL  +     IW+A++ + Q +      PF ++++  SL VVYLP+      ++D 
Sbjct: 7   RWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLEDA 66

Query: 163 VCSLLNPDLLNDH-HENDGSVPSSSIGLDVPLKFNEIHYKFDEDLKSCLTSDKDLSEREE 222
             SLL       H  E   S  +  +G DV              +KS  T    L  RE+
Sbjct: 67  YGSLLFWRSKRSHLMELVESEKAVLLGKDV------------SGVKSDATESSGLVVREQ 126

Query: 223 -----GQPLMSNFES--------------HHKVSTREIIER-----------SLYLTPIW 282
                G  + S  E+                 VS + + E+           SL + P W
Sbjct: 127 EISEGGNGIESGLENIELEIDASVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFW 186

Query: 283 FTTEYFSNSALANTSVATATILNSTSGLFALLFGALVGQESITITKVVAVFISMAGVVMT 342
           F  +   N +L  T+V + TIL+S S LF  L   +   E  T  K+ +V + M+G ++ 
Sbjct: 187 FLAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIV 246

Query: 343 TLGKTWATNGFPVISESRGRTIT-----GDVFCLLSAAIYGLFTVLLKKLAGSGGD---K 402
           ++G           SES+   +      GD+  L+SAA+Y ++  L++K      +   +
Sbjct: 247 SMGD----------SESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGR 306

Query: 403 IDVQKFFGYVGLFTIVGLWWLVWPLTAMGIEPPFKFPP-SKSITEIVVLNGFVGNVLSDY 462
           + + +F G++GLF     +  + P   +      +F   +     +VV  G + NVLSDY
Sbjct: 307 VSMAQFLGFLGLF---NFFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDY 366

Query: 463 FWALSVIWTSPLVSNLGMSLTIPLAMLADVILHGRRYSTLYIVGCVQVFAGFFIVNFSDK 480
            WA +V+ T+  V+  G+++ +PLA + D  L G + S    +G   V  GF  +N   +
Sbjct: 367 LWAKAVLLTTTTVATAGLTIQVPLAAIVD-SLSGNKPSFTDYIGAAAVMVGFAGINIPSE 401

BLAST of Cp4.1LG02g05630 vs. TAIR 10
Match: AT2G24970.1 (unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 28; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink). )

HSP 1 Score: 45.4 bits (106), Expect = 1.5e-04
Identity = 18/39 (46.15%), Postives = 27/39 (69.23%), Query Frame = 0

Query: 23 SRERHHQAAEGLTNLFPTATHDLSRIQYRLEEEFQQSYP 62
          +   HHQA + L N+F  A+HDL+ +  +L++EFQQ YP
Sbjct: 2  THNHHHQAVDNLLNVFSRASHDLTVVHSKLDKEFQQMYP 40

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5R6J31.7e-3729.98Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1[more]
Q037302.9e-3729.31Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (s... [more]
Q8R3148.4e-3728.74Solute carrier family 35 member F5 OS=Mus musculus OX=10090 GN=Slc35f5 PE=2 SV=1[more]
A6QL921.4e-3629.27Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1[more]
Q8WV832.4e-3629.27Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG7037663.17.62e-29294.32hypothetical protein SDJN02_01293 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023524239.13.01e-277100.00thiamine-repressible mitochondrial transport protein THI74-like isoform X1 [Cucu... [more]
XP_022982173.17.52e-27197.68thiamine-repressible mitochondrial transport protein THI74-like isoform X4 [Cucu... [more]
XP_022940128.15.06e-26997.42thiamine-repressible mitochondrial transport protein THI74-like isoform X3 [Cucu... [more]
XP_022982168.12.13e-26383.12uncharacterized transporter C405.03c-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1J4633.64e-27197.68thiamine-repressible mitochondrial transport protein THI74-like isoform X4 OS=Cu... [more]
A0A6J1FNF42.45e-26997.42thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cu... [more]
A0A6J1J4591.03e-26383.12uncharacterized transporter C405.03c-like isoform X2 OS=Cucurbita maxima OX=3661... [more]
A0A6J1IYL19.56e-25497.54thiamine-repressible mitochondrial transport protein THI74-like isoform X3 OS=Cu... [more]
A0A6J1FHK56.41e-25297.27thiamine-repressible mitochondrial transport protein THI74-like isoform X1 OS=Cu... [more]
Match NameE-valueIdentityDescription
AT4G32140.11.3e-11255.03EamA-like transporter family [more]
AT3G07080.11.0e-2125.65EamA-like transporter family [more]
AT2G24970.11.5e-0446.15unknown protein; Has 33 Blast hits to 33 proteins in 17 species: Archae - 0; Bac... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 251..308
e-value: 6.5E-8
score: 32.8
NoneNo IPR availableGENE3D6.10.250.1380coord: 28..79
e-value: 2.8E-6
score: 29.1
NoneNo IPR availablePANTHERPTHR23051:SF9THIAMINE-REPRESSIBLE MITOCHONDRIAL TRANSPORT PROTEIN THI74-LIKE ISOFORM X1coord: 100..481
NoneNo IPR availablePANTHERPTHR23051SOLUTE CARRIER FAMILY 35, MEMBER F5coord: 100..481
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 371..477
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 249..311
IPR042091Ska2, N-terminalPFAMPF16740SKA2coord: 28..69
e-value: 2.1E-9
score: 37.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g05630.1Cp4.1LG02g05630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane