Cp4.1LG01g24430 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g24430
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionplasma membrane intrinsic protein 2
LocationCp4.1LG01: 19049402 .. 19052706 (+)
RNA-Seq ExpressionCp4.1LG01g24430
SyntenyCp4.1LG01g24430
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCAAAGACGTTAAAAGGTTACCGCCATTGATTATTTATTTGAAGCATGAAAGGAATTTGTCGTTACTGTAGTAAGTGGTATGCACAGTGAAATCACTGTTCTGAGTGGGTCAGAACGACAAAGGTACGGAGCATAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCTGAAACTCACTGCTCCTTTTCTCTTTTCATCCTTCAATTTCCTCTTATTAAATGCAGCTCCTCCCTCCCCAACTTTGCACCATATTCAAATCAAAATCCATCGCCGGAAGTTGATTTTCTCTCTCCTAGGGCTTCGATTTTCGATTTCTCTGTCATTATGGCGAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATACCGGCAACAAGAACAATGATGCTTGCGGTGGCGTTGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTTCTTCTTTCTCCGTTTCTTCGGCTTAAAGTTGAAGTATTGAAATTCTTTTTCCAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCATTTGTTTTCCAATCTTTTTGGGAATGTGAGAGATTGGATGGGAGAGAGGTATGAGTAATTGTATTTTTATTTATTATTATTATTATTTTAAAACCATAGAATGAATATTGAAAAATAATCTAAGTGACCCCAATGTTAGAGTCGAGAAATGCACACATGCAAATATGATTTCGTGTCTAGTGGCATAAAACATCATGTCTTGGCTTTGGCTCTAATTTTTTTTTTTTTTTTTTTTTTTTGGTTGAGAGTTCATGATATTATCATGCACTTTGTTTACGTTATTCCATTTGGCGCTTACACATTATTATTATTATTATTATTATTATTTTTTGTGTAAAAATCATTTTTAAATCAATATTTTATTGATTTTAGATCCTCATTTTAAAATTTATACCCAGTTGAAAATGTAAACAAAAATATCAAAATTCGTATGTAATAGAAATATTTATAAAAATTTTGAAAAAAAAATACTGATAGAAATTCCTATAAATATCATATTTTTATTTAATTTTTTTAAAGTATATACAAATTATGCAAATTTGAATAAAATTGTAACAAAATCCAAATATTCCTTCTCATTTATTAAACTATAGTTTATATATAGGAAATTATATATTAATTTTATTATTATTTTTTTCCATTTAAAGAAAAGCTTTTAATATTTCAAATTTTTTCTTGTATTATTGTAATAAACCACAAATTAGAATCTTTCGACGGTCATATTTAATCTCCAATATAAATATATAATATTGTGATATCCCATTGGGAAGAGAACAAAACACTCTTGGTAAGGGTGTGGAAACTTTCCCGAGCAATTGTTTTAAAGCCTTGAGGGGAAGCCTGAAAGGAAAAGACCAATGAGGACAATATTTGTTAGCGGTGAGCTTGAGTCGTTACAAATATTTTAATTATTTAGCAGTTGGTCATAATTTTTAATTTTGAGTTTATTTTTTTAGAAAAATGAGAGAAAAGAAAGTTAGTGTTGACTTGAGGAGGATGTGCAGGTGGGCATATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGTTTAGGGGCCATTTGTGGAGTTGGGCTCGTGAAGTCCTTCCAAAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTAGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTATGCATCTTTAATTTTAATTTTAATTTTAATCTCATAAATTATATTTAAAAGATTAAAAATATTTATATTTTAATATTTTTTATAATCTTAAATATTAAATTTTATTATTTCTACTTTCAATAAAAAGTTAAAATTAGTTTATTCTTCAATTTTGTAAAAAATAAAATAAAAAACAAATAATTTCTATATATATATATATATTTTTTGGGTATTTTATTTTAATGGTCAGTTAATTTTCTTGTTATTGAAAGAATTATAAAGTGGGTTCATGTGTTAGGCACCAACTTTCACCTGTAGATGCCTCTTTCAGAACTTACTTTCTTACAGATAATAATAATAAATAAATAAATAAATAAATTACTGTTATCTTTGACCAAACTTTATTTTACTTGGCTTCACATGCATACAAATCCTTATCTTCTTTTTTTGTCATTGAAGGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCAGCTAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCACGTTAGTTTCTATTTTTATCTATGGTCGAACTTTATTTAAATATCTATTAATTTAAACAGAACTTAACTAGTTAAGGTATGTACTCCGACTAATAGACTAGAAGTTTGAATCCCTCTATTACCACATGCTGTTTATACTCACTAATAGACTAGAAGTTTGAGTCCCTCCATTCCCATATGTTTATATTCTAACTAAAGACGAGAAGCTCGAGTCCCTCCATTCCCACATATTGGTTCTACTCTGATTAATAGGCTAGAAGTTCGAGTCTACCCTGACTAATAGACTAGAAGTTCGAGTCCCTCCATTCTCACATGTTGTTTATACTCTGACTAATAGACTAGAAGTTCGAGTCCTTCCATTCTCACATGTTATCGAACTAAAAAATCCATCTAAACTTCTTGATTTGATAATGTTTCATAAAAGAATCCAACATTTCAAAAAGAGAAACGTTGATGACTCAAATATTTGGTTTTGGGAACTAATTAGTATGGATACTTGTTTGCAGTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCCTGCGAGCAGGTGCTGTGAAGGCTCTTGGGTCATTTAGAAGCAACCCAAATGTGTGATGATCATAATGCTGCCTTTAATTAGCTCCTAGAGATTTAAGAACCAGATGTTAACACTAGT

mRNA sequence

GCAAAGACGTTAAAAGGTTACCGCCATTGATTATTTATTTGAAGCATGAAAGGAATTTGTCGTTACTGTAGTAAGTGGTATGCACAGTGAAATCACTGTTCTGAGTGGGTCAGAACGACAAAGGTACGGAGCATAGCAATGGCAAGTGGGTGGGCGGTGTGCCCAACTCAGCCGGAGCCGCTGAAACTCACTGCTCCTTTTCTCTTTTCATCCTTCAATTTCCTCTTATTAAATGCAGCTCCTCCCTCCCCAACTTTGCACCATATTCAAATCAAAATCCATCGCCGGAAGTTGATTTTCTCTCTCCTAGGGCTTCGATTTTCGATTTCTCTGTCATTATGGCGAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATACCGGCAACAAGAACAATGATGCTTGCGGTGGCGTTGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTGGGCATATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGTTTAGGGGCCATTTGTGGAGTTGGGCTCGTGAAGTCCTTCCAAAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTAGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCAGCTAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCACTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCCTGCGAGCAGGTGCTGTGAAGGCTCTTGGGTCATTTAGAAGCAACCCAAATGTGTGATGATCATAATGCTGCCTTTAATTAGCTCCTAGAGATTTAAGAACCAGATGTTAACACTAGT

Coding sequence (CDS)

ATGGCGAAGAATGCAGCGGAACGCGGCTCGTTCTCGGCGAAGGACTACCACGATCCACCACCGGCGCCGCTCATCGATGCCGTGGAGATTACGAAATGGTCGTTTTACAGAGCTCTGATTGCGGAGTTCATAGCCACTCTGCTTTTCCTGTACGTCACTGTGTTGACGGTGATCGGCTACAAATCGCAGACGGATCATACCGGCAACAAGAACAATGATGCTTGCGGTGGCGTTGGGATTCTTGGCATTGCCTGGGCGTTTGGTGGAATGATCTTCGTTCTTGTTTACTGTACCGCTGGAATTTCAGGTGGGCATATAAATCCAGCAGTGACATTTGGGCTTTTGTTGGCCCGAAAGGTGTCACTTGTCCGGGCCGTGATGTACATGGTGGCCCAATGTTTAGGGGCCATTTGTGGAGTTGGGCTCGTGAAGTCCTTCCAAAAGGCCCACTTCCAGAAGTACGGCGGTGGGGCAAATGGGCTCGCCGACGGCTACACCGTCGGCGTTGGACTGGCGGCGGAGATCGTAGGGACGTTTGTGTTGGTGTATACAGTCTTCTCTGCTACAGATCCCAAACGAAGCGCAAGAGATTCCCACGTTCCTGTTTTGGCGCCACTCCCAATTGGATTTGCAGTGTTCATAGTTCACCTGGCCACCATTCCAGTCACTGGCACTGGAATCAACCCAGCTAGGAGCTTGGGATCTGCTGTTATTTTCAACCACCAAAACACTTGGAATAATCACTGGATATTTTGGGTTGGACCCTTCATAGGAGCAGCCATTGCAGCTTTCTACCACCAATTCATCCTGCGAGCAGGTGCTGTGAAGGCTCTTGGGTCATTTAGAAGCAACCCAAATGTGTGA

Protein sequence

MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Homology
BLAST of Cp4.1LG01g24430 vs. ExPASy Swiss-Prot
Match: Q9SV31 (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 6.3e-133
Identity = 231/281 (82.21%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 7   ERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 66
           ++ SFS KDY DPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 67  TGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 126
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 127 MYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 186
           MYMVAQCLGAICGV LVK+FQ A+F +YGGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 187 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWN 246
           SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIP+TGTGINPARSLG+A+I+N    W+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 247 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS P+V
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of Cp4.1LG01g24430 vs. ExPASy Swiss-Prot
Match: Q8H5N9 (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 5.9e-131
Identity = 226/276 (81.88%), Postives = 254/276 (92.03%), Query Frame = 0

Query: 9   GSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDHTG 68
           G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK QTD + 
Sbjct: 14  GEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASA 73

Query: 69  NKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAVMY 128
           +  + ACGGVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKVSLVRA++Y
Sbjct: 74  SGADAACGGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILY 133

Query: 129 MVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVFSA 188
           +VAQCLGAICGVGLVK+FQ A+F +YGGGAN LA GY+ G GLAAEI+GTFVLVYTVFSA
Sbjct: 134 IVAQCLGAICGVGLVKAFQSAYFNRYGGGANTLAAGYSKGTGLAAEIIGTFVLVYTVFSA 193

Query: 189 TDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWNNH 248
           TDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS+G+AVIFN++  W+NH
Sbjct: 194 TDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSIGAAVIFNNEKAWHNH 253

Query: 249 WIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 285
           WIFWVGPF+GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 254 WIFWVGPFVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of Cp4.1LG01g24430 vs. ExPASy Swiss-Prot
Match: Q84RL7 (Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 462.2 bits (1188), Expect = 4.2e-129
Identity = 226/282 (80.14%), Postives = 253/282 (89.72%), Query Frame = 0

Query: 3   KNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKS 62
           ++ A  G F+AKDY DPPPAPLIDA E+  WS YRA+IAEFIATLLFLY+TV TVIGYK 
Sbjct: 8   ESGAGGGEFAAKDYTDPPPAPLIDAAELGSWSLYRAVIAEFIATLLFLYITVATVIGYKH 67

Query: 63  QTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSL 122
           QTD + +  + ACGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LARKVSL
Sbjct: 68  QTDASASGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSL 127

Query: 123 VRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLV 182
           VRA++Y+VAQCLGAICGVGLVK+FQ A+F +YGGGAN LA GY+ G GL AEI+GTFVLV
Sbjct: 128 VRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANSLASGYSRGTGLGAEIIGTFVLV 187

Query: 183 YTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQ 242
           YTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPVTGTGINPARSLG+AVI+N  
Sbjct: 188 YTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVIYNKD 247

Query: 243 NTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 285
             W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 248 KPWDDHWIFWVGPLVGAAIAAFYHQYILRAGAIKALGSFRSN 289

BLAST of Cp4.1LG01g24430 vs. ExPASy Swiss-Prot
Match: Q9ATM8 (Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 2.1e-128
Identity = 227/286 (79.37%), Postives = 254/286 (88.81%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           + ++ A  G F+AKDY DPPPAPL+DA E+  WS YRA+IAEFIATLLFLYVTV TVIGY
Sbjct: 6   VVQSGAGGGEFAAKDYTDPPPAPLVDAAELGSWSLYRAVIAEFIATLLFLYVTVATVIGY 65

Query: 61  KSQTD--HTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 120
           K QTD   +G   + ACGGVG+LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LAR
Sbjct: 66  KHQTDASASGAGADAACGGVGVLGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLAR 125

Query: 121 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 180
           KVSLVRA++YMVAQCLGA+CGVGLVK+FQ A+F +YGGGAN LA GY+ G GL AEIVGT
Sbjct: 126 KVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANSLASGYSRGAGLGAEIVGT 185

Query: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 240
           FVLVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIPVTGTGINPARSLG+AV+
Sbjct: 186 FVLVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPVTGTGINPARSLGAAVV 245

Query: 241 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 285
           +N    W++HWIFWVGP +GAAIAAFYHQ+ILRAGA+KALGSFRSN
Sbjct: 246 YNKDKPWDDHWIFWVGPLLGAAIAAFYHQYILRAGAIKALGSFRSN 291

BLAST of Cp4.1LG01g24430 vs. ExPASy Swiss-Prot
Match: Q9ATM7 (Aquaporin PIP2-3 OS=Zea mays OX=4577 GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 7.4e-126
Identity = 223/278 (80.22%), Postives = 248/278 (89.21%), Query Frame = 0

Query: 7   ERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 66
           E G FSAKDY DPPPAPLIDA E+TKWS YRA+IAEFIATLLFLY+TV TVIGYK QTD 
Sbjct: 12  EAGEFSAKDYTDPPPAPLIDADELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDA 71

Query: 67  TGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 126
             +  + ACGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKVSLVRA+
Sbjct: 72  AASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAL 131

Query: 127 MYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 186
           +Y++AQCLGAICGVGLVK FQ A++ +YGGGAN L+DGY+ G GLAAEI+GTFVLVYTVF
Sbjct: 132 LYIIAQCLGAICGVGLVKGFQSAYYVRYGGGANELSDGYSKGTGLAAEIIGTFVLVYTVF 191

Query: 187 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWN 246
           SATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N    W+
Sbjct: 192 SATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYNKDKAWD 251

Query: 247 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSN 285
           + WIFWVGP IGAAIAA YHQ++LRA A K LGS+RSN
Sbjct: 252 DQWIFWVGPLIGAAIAAAYHQYVLRASATK-LGSYRSN 288

BLAST of Cp4.1LG01g24430 vs. NCBI nr
Match: XP_023540772.1 (probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 576 bits (1485), Expect = 2.23e-207
Identity = 287/287 (100.00%), Postives = 287/287 (100.00%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Cp4.1LG01g24430 vs. NCBI nr
Match: XP_022954319.1 (probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquaporin PIP2-5 [Cucurbita maxima] >KAG6602317.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033000.1 putative aquaporin PIP2-5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 568 bits (1465), Expect = 2.50e-204
Identity = 284/287 (98.95%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDH GNKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Cp4.1LG01g24430 vs. NCBI nr
Match: XP_022134214.1 (probable aquaporin PIP2-5 [Momordica charantia])

HSP 1 Score: 542 bits (1397), Expect = 5.81e-194
Identity = 269/287 (93.73%), Postives = 277/287 (96.52%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFS KDYHDPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDHT NKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQKYGGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAV +VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
            Q TWN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of Cp4.1LG01g24430 vs. NCBI nr
Match: KDP44363.1 (hypothetical protein JCGZ_20043 [Jatropha curcas])

HSP 1 Score: 503 bits (1296), Expect = 1.33e-178
Identity = 248/287 (86.41%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           M K+ AE+GSFSAKDYHDPPPAPLIDA E+TKWSFYRALIAEFIATLLFLY+TVLTVIGY
Sbjct: 1   MVKDVAEQGSFSAKDYHDPPPAPLIDAEELTKWSFYRALIAEFIATLLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTD    KN D+CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KSQTDPA--KNADSCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQ LGAICGVGLVK+FQ A++++YGGGAN LADGY+ GVGL AEI+GTFV
Sbjct: 121 SLVRAVMYMVAQSLGAICGVGLVKAFQSAYYKRYGGGANTLADGYSTGVGLGAEIIGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           H   W++ WIFWVGPF+GAAIAAFYHQFILRAGAVKALGSFRSNP V
Sbjct: 241 HDKAWDDQWIFWVGPFLGAAIAAFYHQFILRAGAVKALGSFRSNPTV 285

BLAST of Cp4.1LG01g24430 vs. NCBI nr
Match: XP_038678956.1 (probable aquaporin PIP2-5 [Tripterygium wilfordii])

HSP 1 Score: 501 bits (1289), Expect = 1.55e-177
Identity = 248/287 (86.41%), Postives = 267/287 (93.03%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAK+ AE+GSFSAKDYHDPPPAPL DAVE+TKWSFYRALIAEFIATLLFLY+TVLTVIGY
Sbjct: 1   MAKDIAEQGSFSAKDYHDPPPAPLFDAVELTKWSFYRALIAEFIATLLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDH    N DACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KSQTDHA--LNADACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAI GV LVK+FQK+ +++YGGGAN LADGY+ G GLAAEI+GTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVALVKAFQKSFYKRYGGGANTLADGYSTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
               W++ WIFWVGPFIGAAIAAFYHQFILRAGA KALGSFRSNP+V
Sbjct: 241 QDKAWDDQWIFWVGPFIGAAIAAFYHQFILRAGAAKALGSFRSNPSV 285

BLAST of Cp4.1LG01g24430 vs. ExPASy TrEMBL
Match: A0A6J1JQ27 (probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 1.21e-204
Identity = 284/287 (98.95%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDH GNKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Cp4.1LG01g24430 vs. ExPASy TrEMBL
Match: A0A6J1GSM7 (probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=1)

HSP 1 Score: 568 bits (1465), Expect = 1.21e-204
Identity = 284/287 (98.95%), Postives = 284/287 (98.95%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY
Sbjct: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDH GNKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHIGNKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV
Sbjct: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287

BLAST of Cp4.1LG01g24430 vs. ExPASy TrEMBL
Match: A0A6J1BY62 (probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV=1)

HSP 1 Score: 542 bits (1397), Expect = 2.81e-194
Identity = 269/287 (93.73%), Postives = 277/287 (96.52%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAKNAAERGSFS KDYHDPPPAPLIDAVEITKWSFYRALIAEFIAT+LFLY+TVLTVIGY
Sbjct: 1   MAKNAAERGSFSGKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATMLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTDHT NKN D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV
Sbjct: 61  KSQTDHTANKNVDPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQCLGAI GVGLVK+FQKAHFQKYGGGANGLADGYT GVGLAAEIVGTFV
Sbjct: 121 SLVRAVMYMVAQCLGAISGVGLVKAFQKAHFQKYGGGANGLADGYTTGVGLAAEIVGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAV +VHLATIP+TGTGINPARSLGSAVI N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVLMVHLATIPITGTGINPARSLGSAVILN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
            Q TWN+HWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 DQKTWNDHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of Cp4.1LG01g24430 vs. ExPASy TrEMBL
Match: A0A067LAW1 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_20043 PE=3 SV=1)

HSP 1 Score: 503 bits (1296), Expect = 6.45e-179
Identity = 248/287 (86.41%), Postives = 268/287 (93.38%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           M K+ AE+GSFSAKDYHDPPPAPLIDA E+TKWSFYRALIAEFIATLLFLY+TVLTVIGY
Sbjct: 1   MVKDVAEQGSFSAKDYHDPPPAPLIDAEELTKWSFYRALIAEFIATLLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           KSQTD    KN D+CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KSQTDPA--KNADSCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SLVRAVMYMVAQ LGAICGVGLVK+FQ A++++YGGGAN LADGY+ GVGL AEI+GTFV
Sbjct: 121 SLVRAVMYMVAQSLGAICGVGLVKAFQSAYYKRYGGGANTLADGYSTGVGLGAEIIGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI+N
Sbjct: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVIYN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           H   W++ WIFWVGPF+GAAIAAFYHQFILRAGAVKALGSFRSNP V
Sbjct: 241 HDKAWDDQWIFWVGPFLGAAIAAFYHQFILRAGAVKALGSFRSNPTV 285

BLAST of Cp4.1LG01g24430 vs. ExPASy TrEMBL
Match: A0A5B6ZA79 (Putative aquaporin PIP2-5 OS=Davidia involucrata OX=16924 GN=Din_009434 PE=3 SV=1)

HSP 1 Score: 500 bits (1288), Expect = 1.15e-177
Identity = 250/289 (86.51%), Postives = 269/289 (93.08%), Query Frame = 0

Query: 1   MAKNA--AERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 60
           MAK+   AE+GSFSAKDYHDPPPAPLID  E+TKWSFYRALIAEFIATLLFLY+TVLTVI
Sbjct: 1   MAKDVGVAEQGSFSAKDYHDPPPAPLIDPDELTKWSFYRALIAEFIATLLFLYITVLTVI 60

Query: 61  GYKSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 120
           GYKSQTD    K  D CGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKSQTDPA--KQADPCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLAR 120

Query: 121 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 180
           KVSLVRAVMYMVAQCLGAICGVGLVK+FQK ++++YGGGAN LADGY+ GVGLAAEI+GT
Sbjct: 121 KVSLVRAVMYMVAQCLGAICGVGLVKAFQKPYYKRYGGGANELADGYSTGVGLAAEIIGT 180

Query: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 240
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARSLG+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSLGAAVI 240

Query: 241 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 287
           +N    W++HW+FWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNP+V
Sbjct: 241 YNQSKAWDDHWMFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPHV 287

BLAST of Cp4.1LG01g24430 vs. TAIR 10
Match: AT3G54820.1 (plasma membrane intrinsic protein 2;5 )

HSP 1 Score: 474.9 bits (1221), Expect = 4.5e-134
Identity = 231/281 (82.21%), Postives = 256/281 (91.10%), Query Frame = 0

Query: 7   ERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGYKSQTDH 66
           ++ SFS KDY DPPP PL DA E+ KWSFYRALIAEFIATLLFLYVT++TVIGYKSQTD 
Sbjct: 8   DKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDP 67

Query: 67  TGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKVSLVRAV 126
               N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGLLLARKV+LVRAV
Sbjct: 68  A--LNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLLARKVTLVRAV 127

Query: 127 MYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFVLVYTVF 186
           MYMVAQCLGAICGV LVK+FQ A+F +YGGGANGL+DGY++G G+AAEI+GTFVLVYTVF
Sbjct: 128 MYMVAQCLGAICGVALVKAFQSAYFTRYGGGANGLSDGYSIGTGVAAEIIGTFVLVYTVF 187

Query: 187 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFNHQNTWN 246
           SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIP+TGTGINPARSLG+A+I+N    W+
Sbjct: 188 SATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPITGTGINPARSLGAAIIYNKDKAWD 247

Query: 247 NHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
           +HWIFWVGPF GAAIAAFYHQF+LRAGA+KALGSFRS P+V
Sbjct: 248 HHWIFWVGPFAGAAIAAFYHQFVLRAGAIKALGSFRSQPHV 286

BLAST of Cp4.1LG01g24430 vs. TAIR 10
Match: AT2G37180.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 449.9 bits (1156), Expect = 1.5e-126
Identity = 222/287 (77.35%), Postives = 244/287 (85.02%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAK+      F  +DY DPPP P  DA E+TKWS YRA+IAEF+ATLLFLYVTVLTVIGY
Sbjct: 1   MAKDVEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           K Q+D      +  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAGGVD--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SL+RAV+YMVAQCLGAICGVG VK+FQ +H+  YGGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSHYVNYGGGANFLADGYNTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVIFN
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIFN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
               W++HWIFWVGPFIGA IAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 KSKPWDDHWIFWVGPFIGATIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Cp4.1LG01g24430 vs. TAIR 10
Match: AT2G37170.1 (plasma membrane intrinsic protein 2 )

HSP 1 Score: 449.5 bits (1155), Expect = 2.0e-126
Identity = 220/287 (76.66%), Postives = 246/287 (85.71%), Query Frame = 0

Query: 1   MAKNAAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVIGY 60
           MAK+      F  +DY DPPP P  DA E+TKWS YRA+IAEF+ATLLFLY+TVLTVIGY
Sbjct: 1   MAKDVEGPEGFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGY 60

Query: 61  KSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLARKV 120
           K Q+D      +  CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LARKV
Sbjct: 61  KIQSDTKAGGVD--CGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKV 120

Query: 121 SLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGTFV 180
           SL+RAV+YMVAQCLGAICGVG VK+FQ +++ +YGGGAN LADGY  G GLAAEI+GTFV
Sbjct: 121 SLIRAVLYMVAQCLGAICGVGFVKAFQSSYYDRYGGGANSLADGYNTGTGLAAEIIGTFV 180

Query: 181 LVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVIFN 240
           LVYTVFSATDPKR+ARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI+N
Sbjct: 181 LVYTVFSATDPKRNARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVIYN 240

Query: 241 HQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
               W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 KSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 285

BLAST of Cp4.1LG01g24430 vs. TAIR 10
Match: AT3G53420.1 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 445.7 bits (1145), Expect = 2.9e-125
Identity = 220/289 (76.12%), Postives = 248/289 (85.81%), Query Frame = 0

Query: 1   MAKN--AAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 60
           MAK+  A     F  +DY DPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 61  GYKSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 120
           GYK Q+D   +     CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSD--TDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 121 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 180
           KVSL RA++Y++AQCLGAICGVG VK+FQ +++ +YGGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 240
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 241 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
           +N    W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

BLAST of Cp4.1LG01g24430 vs. TAIR 10
Match: AT3G53420.2 (plasma membrane intrinsic protein 2A )

HSP 1 Score: 445.7 bits (1145), Expect = 2.9e-125
Identity = 220/289 (76.12%), Postives = 248/289 (85.81%), Query Frame = 0

Query: 1   MAKN--AAERGSFSAKDYHDPPPAPLIDAVEITKWSFYRALIAEFIATLLFLYVTVLTVI 60
           MAK+  A     F  +DY DPPPAP ID  E+ KWSFYRA+IAEF+ATLLFLY+TVLTVI
Sbjct: 1   MAKDVEAVPGEGFQTRDYQDPPPAPFIDGAELKKWSFYRAVIAEFVATLLFLYITVLTVI 60

Query: 61  GYKSQTDHTGNKNNDACGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLLLAR 120
           GYK Q+D   +     CGGVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTFGL LAR
Sbjct: 61  GYKIQSD--TDAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLAR 120

Query: 121 KVSLVRAVMYMVAQCLGAICGVGLVKSFQKAHFQKYGGGANGLADGYTVGVGLAAEIVGT 180
           KVSL RA++Y++AQCLGAICGVG VK+FQ +++ +YGGGAN LADGY+ G GLAAEI+GT
Sbjct: 121 KVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANSLADGYSTGTGLAAEIIGT 180

Query: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFIVHLATIPVTGTGINPARSLGSAVI 240
           FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVF+VHLATIP+TGTGINPARS G+AVI
Sbjct: 181 FVLVYTVFSATDPKRSARDSHVPVLAPLPIGFAVFMVHLATIPITGTGINPARSFGAAVI 240

Query: 241 FNHQNTWNNHWIFWVGPFIGAAIAAFYHQFILRAGAVKALGSFRSNPNV 288
           +N    W++HWIFWVGPFIGAAIAAFYHQF+LRA   K+LGSFRS  NV
Sbjct: 241 YNKSKPWDDHWIFWVGPFIGAAIAAFYHQFVLRASGSKSLGSFRSAANV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SV316.3e-13382.21Probable aquaporin PIP2-5 OS=Arabidopsis thaliana OX=3702 GN=PIP2-5 PE=1 SV=1[more]
Q8H5N95.9e-13181.88Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PIP2-1 PE=... [more]
Q84RL74.2e-12980.14Aquaporin PIP2-1 OS=Zea mays OX=4577 GN=PIP2-1 PE=1 SV=2[more]
Q9ATM82.1e-12879.37Aquaporin PIP2-2 OS=Zea mays OX=4577 GN=PIP2-2 PE=2 SV=1[more]
Q9ATM77.4e-12680.22Aquaporin PIP2-3 OS=Zea mays OX=4577 GN=PIP2-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023540772.12.23e-207100.00probable aquaporin PIP2-5 [Cucurbita pepo subsp. pepo][more]
XP_022954319.12.50e-20498.95probable aquaporin PIP2-5 [Cucurbita moschata] >XP_022990425.1 probable aquapori... [more]
XP_022134214.15.81e-19493.73probable aquaporin PIP2-5 [Momordica charantia][more]
KDP44363.11.33e-17886.41hypothetical protein JCGZ_20043 [Jatropha curcas][more]
XP_038678956.11.55e-17786.41probable aquaporin PIP2-5 [Tripterygium wilfordii][more]
Match NameE-valueIdentityDescription
A0A6J1JQ271.21e-20498.95probable aquaporin PIP2-5 OS=Cucurbita maxima OX=3661 GN=LOC111487288 PE=3 SV=1[more]
A0A6J1GSM71.21e-20498.95probable aquaporin PIP2-5 OS=Cucurbita moschata OX=3662 GN=LOC111456599 PE=3 SV=... [more]
A0A6J1BY622.81e-19493.73probable aquaporin PIP2-5 OS=Momordica charantia OX=3673 GN=LOC111006527 PE=3 SV... [more]
A0A067LAW16.45e-17986.41Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_20043 PE=3 SV=1[more]
A0A5B6ZA791.15e-17786.51Putative aquaporin PIP2-5 OS=Davidia involucrata OX=16924 GN=Din_009434 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G54820.14.5e-13482.21plasma membrane intrinsic protein 2;5 [more]
AT2G37180.11.5e-12677.35Aquaporin-like superfamily protein [more]
AT2G37170.12.0e-12676.66plasma membrane intrinsic protein 2 [more]
AT3G53420.12.9e-12576.12plasma membrane intrinsic protein 2A [more]
AT3G53420.22.9e-12576.12plasma membrane intrinsic protein 2A [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 87..111
score: 62.61
coord: 173..191
score: 53.95
coord: 37..56
score: 45.9
coord: 249..269
score: 50.21
coord: 124..143
score: 50.83
coord: 209..231
score: 56.93
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 41..266
e-value: 7.7E-64
score: 213.5
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 29..266
e-value: 8.4E-83
score: 277.5
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 37..269
e-value: 1.65331E-72
score: 220.588
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 22..287
e-value: 9.0E-88
score: 295.6
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 29..271
IPR034294Aquaporin transporterPANTHERPTHR45687AQUAPORIN OR AQUAGLYCEROPORIN RELATEDcoord: 5..284
NoneNo IPR availablePANTHERPTHR45687:SF40AQUAPORIN PIP2-1-RELATEDcoord: 5..284
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 105..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g24430.1Cp4.1LG01g24430.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity