Cp4.1LG01g23900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g23900
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	very-long-chain enoyl-CoA reductase-like
Location	Cp4.1LG01: 19391559 .. 19395980 (+)
RNA-Seq Expression	Cp4.1LG01g23900
Synteny	Cp4.1LG01g23900

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAGAAAGCCCAAAGCAATATATATAATATATACTAAGGGCTGTTGTTCTGAACCCCATTGATCCGAGGAGATAGTCAAATTAGAAGACAGTAGGCAGAAAGTATAAGATGTGCCTTCATCAAACAGGTTGCAGGCATTGTCAAGTTAAATTGCACACGGCACACACACCTAATTTCTGAAAAAAGAAGAAGCTGAAATTGATATCTGATTTCAAGAACATAGCTAGACAACTGTTGATAATATCATTGATCAGAAACAGAAATGGAAGTCCTATACTCAAATAAGAAGACAATAGGCAAAAAATAAAAGGTGAACATTTCATCAAACAGGTTATAGGCATAATCAAGATAAATTGCACCCAGCAATCGTCACATACATGACCATATTTCCGACTATGAATAATCATCAGAGAGTGACTCTACGTATTTAAGAACATAACAGATTGAGAGAATACAAGATTTGGTTTGTAGATGTTGTGGACGACGGAGGCGCCGGCGAGAAGCGAAGAGATGACGACGACGAAGGACCAACCCACCGAGGGGGTTCCAGTGGGGAGCAACCGACCACCGAATTTCTGAGCCATTATTCAGCTCAACACCGTACAAAGATATCAATTTCTCACCCTAAAAAGGTTTCATTATTCAATCTGCCCGCCAACTGCCCTTTAAATAACCGGTCCAAGGCTCCGGCCCGGCTCCAGCTTCGCAAACCCGTTGTTTTTTTTTTTTTTTTTGTTACATTTTCGATTTTGGGTTAAATATTCAATTTAGTATGTTTTAACATTTTGAATGCGTCGAATTGTAAATTTGTACTGAGGAGATGACTAGGAGGAACGCGCTGCATCCGTCGTTGGAATTAGCTCTCCTACCCCAACTGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCCACTTCAAACTCAGAGAACTTTCGCCATTTTTTAATAAAATCCCTTAGCAGATCTCAGCGTATAACCATTTCTGGGTTCTGGGTCCTCTGATTCTGCTATTTCAGGATCTGGGTTGAAATCACAGTGGGGTTAATTTGGCTGTGAAGATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGTAAGTGTTCGGATTTTGATTCAATTACTGTTTCTTTCTCGATTCTTGGAATACAAGAAAAGATGATAAGAGTACAACTTGTTTTGGTGGCTGTGTTCTNTGAGTAGCTGATGGGATCTTAAGTTTCATGGACATTGTTGTTTAATCCTTTGCAGGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAAGTGAGTACATGAGGGTAGAATGTTTTCTTTAATTGTTGCATACATGTTCTTTTTAGCTTAGAATTGTTAGAAATCTTAGCTTAGATTTGACATTTTTGTTGGAGTGAGTGATAGAACTGGACTTGTTGTTAAACGGATGAATCTAAAGATTAAGTTTAGCCTAGCACAACAGTTTAAGCTTTTGGGTGTGAAACTGTAACAGGTATGCAGACATATTGTCATGTCCTCTTTGAGCTTCACTTTTCGGGCTTTGGCTCAAGGTTTTAAACGTGTCGGCTNGCTATGCAGATATTGTCATGTCCTCTTTGAGCTTCACTTTTCGGGCTTTGGCTCACTTTTCGGGCTTTGGCTCAAGGTTTTAAACGTGTCGGCTAGGGAGAGGTTTTCACATCCTTATAAAGAATGCTTCGTTCTCCTTCCAACCAATGTGCGATCTCACAATTCACCCCCATTTGGGCCAACGAATCTTACAATCCACCTCCGTTTTCCTCCCCAACCGACGTGGAATCTCAAAATCCACCCCCTTCAAGGCTCAGCGTCTTTGTTGACATTCTTTCCCTTCTTCAATAGATGAGGGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCTCCTTCGGAGCTCAACCTCCTCACTGGCGCATCACCCCAGTGTCTGGCTCTAATACTATTTGTAACAGTCCAAACCTACTGGATATTGTCCTCTCTGGACTTTCCCTTCAAAGCTTCCCTCCAAANCGAATCTTACAATCCACCTCCGTTTTCCTCCCCAACCGATGTGGAATCTCACAATCCACCCCCCTTCAAGGCTCAACGTCTTTGTTGACATTCTTTCCCTTCTCCAATAGATGAGAGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCCCCCTTCGGAGCTCAACCTCCTCACTGGCGCATCGCCCTGGTGTCTGGCTTTAATACTATTTGTAACAGTCCAAACTTACTGGATATTGTCCTCTTTGGACTTTTCCTTCAAAGCTTCCATTCAAAGTTTTAAGACGCATAGGAAGANAAGTTTTAAGACGCATCGACTAGGAAGAGGTTTCTACTCTTTTATAAAGAATGTTTTGCAAAAATTGCACAAAATATGTTTCACTACTTCATAATAAAGTATTTCTATCTTNGCTCAACGTCTTTGTTGACATTCTTTCCCTTCTCCAATAGATGAGAGACCCCAATCCACCCTCTATGGGGCCCGACGTCCTTGCTGGAAGACCGCCTCGTTTCCACCCCCCTTCGGAGCTCAACCTCCTCACTGGCGCATCGCCCTGGTGTCTGGCTTTAATACTATTTGTAACAGTCCAAACTTACTGGATATTGTCCTCTTTGGACTTTTCCTTCAAAGCTTCCATTCAAAGTTTTAAGACGCATAGGAAGAGGTTTCTACTCTTTTATAAAGAATGTTTTGCAAAAACTGCACGGCTGACAGGTGAGGAATCAAAATATGTTTCACTACTTCATAATAAAGTATTTCTATCTTATCCTTCCTGAAAATTAGTTCTTTCATTACTGTAAAATGGTTAAATGGTTCTTAAGCAGTAGCAAATTGGTAGTCTTTTAGCATGCATACATGTAAGTCGATATCAATTGTTATGTAAAGTGCAGCTACCTGATTTCGTTGTTTCATTGAAACAGCCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGGTATAGTATATCTAACTTATTTCTCTTAGTTGTTCTTAAGGTTAGGAATCACGAACCTCCACAATGGTATGATATTGTCCACTTTGAACATGTAGTTTTGCTTTTGATTACTCCAGAAGACCCATGGAGATAGTATTCCTTACTTATACACCTATGATCGACCCCTTACTTACTCATGTTTATTTTTCTTTCACATAATGACTTTTCCGTCCCTATGTGNGAAATCACGAACCTCCACGATGGTATGATATTGTTCACTTTGAACATGTAGTTTTGCTTTTGGTTACTCCAGAAGACTAATGGAGATAGTATTCCTTACTTATACACCTATGATCGACCCCTTACTTACTCACGTTTATTTTTCTTTCACATAATGACTTTTCCGTCCCTATGTGCAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAGAAGGATTGATTGGGCGGCCTCAGATCCATATTTTATCGAGTTGGTAGCTCTAAGTTTAAAATGTCCCTTGAAGAACACCACAAGATATCATTTTCTCAATGTATTTGATGTTCTTTGAGGTTGTCTTAACAGTACTTTGCCTTGTTCTTTGTGTTGTATGGGAATCAAAATAAATTATTATTTATTGGGACATTTTTATTAGCTGATTGATATATCATAATTTTGAAGTATAATTAATTTATTCAAATTAA

mRNA sequence

AAGAGAAAGCCCAAAGCAATATATATAATATATACTAAGGGCTGTTGTTCTGAACCCCATTGATCCGAGGAGATAGTCAAATTAGAAGACAGTAGGCAGAAAGTATAAGATGTGCCTTCATCAAACAGGTTGCAGGCATTGTCAAGTTAAATTGCACACGGCACACACACCTAATTTCTGAAAAAAGAAGAAGCTGAAATTGATATCTGATTTCAAGAACATAGCTAGACAACTGTTGATAATATCATTGATCAGAAACAGAAATGGAAGTCCTATACTCAAATAAGAAGACAATAGGCAAAAAATAAAAGGTGAACATTTCATCAAACAGGTTATAGGCATAATCAAGATAAATTGCACCCAGCAATCGTCACATACATGACCATATTTCCGACTATGAATAATCATCAGAGAGTGACTCTACGTATTTAAGAACATAACAGATTGAGAGAATACAAGATTTGGTTTGTAGATGTTGTGGACGACGGAGGCGCCGGCGAGAAGCGAAGAGATGACGACGACGAAGGACCAACCCACCGAGGGGGTTCCAGTGGGGAGCAACCGACCACCGAATTTCTGAGCCATTATTCAGCTCAACACCGTACAAAGATATCAATTTCTCACCCTAAAAAGGTTTCATTATTCAATCTGCCCGCCAACTGCCCTTTAAATAACCGGTCCAAGGCTCCGGCCCGGCTCCAGCTTCGCAAACCCGTTGTTTTTTTTTTTTTTTTTGTTACATTTTCGATTTTGGGTTAAATATTCAATTTAGTATGTTTTAACATTTTGAATGCGTCGAATTGTAAATTTGTACTGAGGAGATGACTAGGAGGAACGCGCTGCATCCGTCGTTGGAATTAGCTCTCCTACCCCAACTGCACGCCATTTAATAAGCCTACTTCCCAACCTCCCCCTTCAAAACCCACTTCAAACTCAGAGAACTTTCGCCATTTTTTAATAAAATCCCTTAGCAGATCTCAGCGTATAACCATTTCTGGGTTCTGGGTCCTCTGATTCTGCTATTTCAGGATCTGGGTTGAAATCACAGTGGGGTTAATTTGGCTGTGAAGATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAACCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAGAAGGATTGATTGGGCGGCCTCAGATCCATATTTTATCGAGTTGGTAGCTCTAAGTTTAAAATGTCCCTTGAAGAACACCACAAGATATCATTTTCTCAATGTATTTGATGTTCTTTGAGGTTGTCTTAACAGTACTTTGCCTTGTTCTTTGTGTTGTATGGGAATCAAAATAAATTATTATTTATTGGGACATTTTTATTAGCTGATTGATATATCATAATTTTGAAGTATAATTAATTTATTCAAATTAA

Coding sequence (CDS)

ATGAAGGTCTCAGTAGTTTCTCGCAGTGGGAGAGAAGTTGTTGACGGCGGCATTCAACTAAGCGATTCTGCTTCGGTGGCGGATCTGCAGGATGCGATTTACAACCGAACCAAAAAGTTCTACCCTGCTAGGCAGCGGTTAACTCTTCCAGTTCAACCTGGTTCAAAGGAGAGGCCTACTAGAGAGTATTGTAGCAACAACTCAGACAACATAACTGTTGTCTTCAAGGACTTGGGTCCACAAGTTTCTTATCGTACTCTCTTCTTTTTTGAATACTTGGGCCCCTTGATCCTCTACCCCATCTTTTACTACTTCCCTGTCTATCAGTTCTTTGGCTACAAGGCCGAACGTGTCATCCATCCGGTTCAGACGTATGCAATGTACTACTGGTGTTTTCACTACTTCAAACGAATCATGGAAACATTTTTCGTTTCGTATATTGCATATTATGTTAATCACCCACTTTATAGCCCTGTTGGTGACCTTCAAATGAAGATTGGGTTTGCATTTGGTTTGCTGTGTCAAGTGTGTAACTTCTACTGTCATATTTTGCTGAGAAAACTGCGGAGCCCTGAGGGGAATGGGGGATATCAAATCCCAAAGGGGTTTCTGTTCAACATTGTGACATGTGCAAACTACACAACAGAAATATATCAGTGGGTAGGCTTCAATATAGCTACACAGACTGTCGCTGGTTACATCTTTCTTGTGGTTGCTACTCTGATCATGACGAACTGGGCCCTTGCAAAGCACCGTCGCCTCAAGAAGCTGTTTGATGGGAAGGAGGGAAGGCCAAGGTACCCACGCAGATGGGTGATACTTCCTCCATTTCTGTAG

Protein sequence

MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTREYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFVSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL

Homology

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot
Match: Q9M2U2 (Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 8.5e-127
Identity = 217/310 (70.00%), Postives = 250/310 (80.65%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+   + L DSA+VADLQ+A + R KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYC  N++++TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFV                        +YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PV DLQMKIGF FGL+CQV NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot
Match: Q55C17 (Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn2 PE=3 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.3e-37
Identity = 107/309 (34.63%), Postives = 159/309 (51.46%), Query Frame = 0


Query: 3   VSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTR- 62
           + VVS+  ++ V G    S S +V +L+  I ++T+      +R+ L V   + + P   
Sbjct: 4   IKVVSQRSKKEV-GSFSTSSSTTVGELKKQISSKTRL---GTERIRLAVPSKTSKLPNAF 63

Query: 63  EYCSNNSDNI--------TVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGY 122
           E    +SD +        T+ FKDLGPQ+S+  +F  EY GPL +YPIFY+      +G 
Sbjct: 64  EALGKDSDLVSKHVGADSTLYFKDLGPQISWSLVFICEYAGPLFVYPIFYFLS-NLIYGT 123

Query: 123 KAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIAY 182
            + +     Q  A+  +  HY KRI ET FV                        + ++Y
Sbjct: 124 DSPKSF--AQKVALVCYSLHYIKRIYETIFVHRFSHGTMPIFNLFKNCSYYWGCTAMVSY 183

Query: 183 YVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVT 242
           +VNHPLY+     ++ +G    ++ +V N+ CHI LR LR P G+   +IP+G LF  V+
Sbjct: 184 FVNHPLYTEAPIERVYLGLGLWIIGEVFNYICHIQLRNLR-PAGSTERKIPRGLLFEFVS 243

Query: 243 CANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPR 279
           C NYT EI  W+GF+I TQT+  +IF ++    M  WA+ KHRR +K F  K     YP+
Sbjct: 244 CPNYTVEILSWIGFSILTQTLTSWIFALMGAAQMWIWAVGKHRRYRKEFGDK-----YPK 299

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot
Match: Q9N5Y2 (Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=6239 GN=art-1 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 1.9e-33
Identity = 93/276 (33.70%), Postives = 133/276 (48.19%), Query Frame = 0


Query: 38  KKFYPARQRLTLPVQPGSKERPTREYCSN---NSDNITVVFKDLGPQVSYRTLFFFEYLG 97
           KK     +R  L V+P  K     +  S+   +S    +  +DLGPQ++++T+F  EY G
Sbjct: 39  KKLKLTEERQALRVEPKGKPLADDQKLSDLGLSSQKAVLYVRDLGPQIAWKTVFMAEYAG 98

Query: 98  PLILYPIFYYFPVYQFFGYKAERVIHPVQTYAMYYWCFHYFKRIMETFFV---------- 157
           PL +YP+FY  P + +        +HP    A + W FHY KR+ ET F+          
Sbjct: 99  PLFVYPLFYLRPTFIYGQAAVNATMHPAVQIAFFAWSFHYAKRLFETQFIHRFGNSTMPQ 158

Query: 158 --------------SYIAYYVNHPLYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKL 217
                         +++AY+VNHPL++P   GDLQ+  G A  ++ +  N   HILLR L
Sbjct: 159 FNLVKNCSYYWGFAAFVAYFVNHPLFTPPAFGDLQVYFGLAGFVISEFGNLSIHILLRNL 218

Query: 218 RSPEGNGGYQIPK------GFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLI 277
           R P G    +IPK        LFN V+C NYT E+  W+ F+I  Q++   IF       
Sbjct: 219 R-PAGTRERRIPKPDGNPLSLLFNYVSCPNYTYEVASWIFFSIMVQSLPAIIFTTAGFAQ 278

Query: 278 MTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 279
           M  WA  KHR   K F      P YP+    + PF+
Sbjct: 279 MAIWAQGKHRNYLKEF------PDYPKNRKAIVPFV 307

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot
Match: Q9NZ01 (Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 7.4e-30
Identity = 102/310 (32.90%), Postives = 152/310 (49.03%), Query Frame = 0


Query: 2   KVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTR 61
           +V ++    RE +    ++   A++A++++       ++YPARQ L L   P  K     
Sbjct: 5   EVEILDAKTREKLCFLDKVEPHATIAEIKNLFTKTHPQWYPARQSLRL--DPKGKSLKDE 64

Query: 62  EYCSN--NSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVI 121
           +           T+ F+DLG Q+S+ T+F  EY GPL +Y + +YF V   +G+K +   
Sbjct: 65  DVLQKLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGHKYDFTS 124

Query: 122 --HPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIAYYVNH 181
             H V   A     FHY KR++ET FV                        +++AYY+NH
Sbjct: 125 SRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINH 184

Query: 182 PLYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPK------GFLF 241
           PLY+P   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP        +LF
Sbjct: 185 PLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTKNPFTWLF 244

Query: 242 NIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRP 276
            +V+C NYT E+  W+GF I TQ +   +F +V    MT WA  KHR   K F     R 
Sbjct: 245 LLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF-----RD 304

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot
Match: Q3ZCD7 (Very-long-chain enoyl-CoA reductase OS=Bos taurus OX=9913 GN=TECR PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-29
Identity = 102/310 (32.90%), Postives = 151/310 (48.71%), Query Frame = 0


Query: 2   KVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPTR 61
           +V ++    RE +    ++   A++A++++       ++YPARQ L L   P  K     
Sbjct: 5   EVEILDAKTREKLCFLDKVEPQATIAEIKNLFTKTHPQWYPARQSLRL--DPKGKSLKDE 64

Query: 62  EYCSN--NSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVI 121
           +           T+ F+DLG Q+S+ T+F  EY GPL +Y + +YF V   +G K +   
Sbjct: 65  DVLQKLPVGTTATLYFRDLGAQISWVTVFLTEYAGPLFIY-LLFYFRVPFIYGRKYDFTS 124

Query: 122 --HPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIAYYVNH 181
             H V   A     FHY KR++ET FV                        +++AYY+NH
Sbjct: 125 SRHTVVHLACICHSFHYIKRLLETLFVHRFSHGTMPLRNIFKNCTYYWGFAAWMAYYINH 184

Query: 182 PLYSP--VGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPK------GFLF 241
           PLY+P   G  Q+K+  A  ++CQ+ NF  H+ LR LR P G+   +IP        +LF
Sbjct: 185 PLYTPPTYGAQQVKLALAIFVICQLGNFSIHMALRDLR-PAGSKTRKIPYPTRNPFTWLF 244

Query: 242 NIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRP 276
            +V+C NYT E+  W+GF I TQ +   +F +V    MT WA  KHR   K F     R 
Sbjct: 245 LLVSCPNYTYEVGSWIGFAIMTQCLPVALFSLVGFTQMTIWAKGKHRSYLKEF-----RD 304

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: XP_023514664.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 581 bits (1498), Expect = 1.15e-209
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIH 120
           REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIH
Sbjct: 61  REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFGYKAERVIH 120

Query: 121 PVQTYAMYYWCFHYFKRIMETFFVSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFY 180
           PVQTYAMYYWCFHYFKRIMETFFVSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFY
Sbjct: 121 PVQTYAMYYWCFHYFKRIMETFFVSYIAYYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFY 180

Query: 181 CHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVAT 240
           CHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVAT
Sbjct: 181 CHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIFLVVAT 240

Query: 241 LIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 278
           LIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL
Sbjct: 241 LIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 278

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: XP_022921854.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita moschata])

HSP 1 Score: 555 bits (1431), Expect = 6.19e-199
Identity = 275/310 (88.71%), Postives = 276/310 (89.03%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIY RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYSPVGD QMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: KAG6602374.1 (Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 554 bits (1428), Expect = 1.77e-198
Identity = 274/310 (88.39%), Postives = 276/310 (89.03%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSV+SRSGREVVDGGIQLSDSASVADLQDAIY RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVLSRSGREVVDGGIQLSDSASVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKTLKEYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYSPVGD QMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: XP_022990641.1 (very-long-chain enoyl-CoA reductase-like [Cucurbita maxima])

HSP 1 Score: 554 bits (1428), Expect = 1.77e-198
Identity = 275/310 (88.71%), Postives = 275/310 (88.71%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVDGGIQLSDSASVADLQD IY RTKKFYPARQRLTLPVQPG KERPT
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDVIYKRTKKFYPARQRLTLPVQPGLKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKSLREYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. NCBI nr
Match: XP_038890322.1 (very-long-chain enoyl-CoA reductase [Benincasa hispida])

HSP 1 Score: 539 bits (1389), Expect = 1.56e-192
Identity = 265/310 (85.48%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAIY RTKKFYPARQRLTLP+QPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIYKRTKKFYPARQRLTLPIQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSDNITV+FKDLGPQVSYRTLFF+EYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKSLREYCSDNSDNITVIFKDLGPQVSYRTLFFWEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQ+ NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQLSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL
Match: A0A6J1E1Q3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111429991 PE=3 SV=1)

HSP 1 Score: 555 bits (1431), Expect = 3.00e-199
Identity = 275/310 (88.71%), Postives = 276/310 (89.03%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIY RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKSLKEYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYSPVGD QMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYSPVGDFQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL
Match: A0A6J1JNI3 (very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111487465 PE=3 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 8.59e-199
Identity = 275/310 (88.71%), Postives = 275/310 (88.71%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVVDGGIQLSDSASVADLQD IY RTKKFYPARQRLTLPVQPG KERPT
Sbjct: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDVIYKRTKKFYPARQRLTLPVQPGLKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNYKKSLREYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL
Match: A0A0A0KNT8 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G517090 PE=3 SV=1)

HSP 1 Score: 539 bits (1389), Expect = 7.55e-193
Identity = 267/310 (86.13%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL
Match: A0A5A7T2H3 (Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold311G00470 PE=3 SV=1)

HSP 1 Score: 538 bits (1385), Expect = 3.07e-192
Identity = 266/310 (85.81%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y+AERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL
Match: A0A1S3C9G7 (very-long-chain enoyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103498153 PE=3 SV=1)

HSP 1 Score: 538 bits (1385), Expect = 3.07e-192
Identity = 266/310 (85.81%), Postives = 273/310 (88.06%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKVSVVSRSGREVV GGIQLSDSASVADLQDAI+ RTKKFYPARQRLTLPVQPGSKERPT
Sbjct: 1   MKVSVVSRSGREVVKGGIQLSDSASVADLQDAIHKRTKKFYPARQRLTLPVQPGSKERPT 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   REYCS+NSD+ITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG
Sbjct: 61  VLNSKKSLREYCSDNSDSITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y+AERVIHPVQTYAMYYWCFHYFKRIMETFFV                        SYIA
Sbjct: 121 YQAERVIHPVQTYAMYYWCFHYFKRIMETFFVHRFSHATSPLSNVFRNCAYYWSFGSYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PVGDLQMKIGFAFGLLCQV NFYCHILLRKLRSPEGNGGYQIPKGFLFNIV
Sbjct: 181 YYVNHPLYTPVGDLQMKIGFAFGLLCQVSNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 278
           TCANYTTEIYQWVGFNIATQT AGY+FLVVA LIMTNWALAKHRRLKKLFDGKEGRP+YP
Sbjct: 241 TCANYTTEIYQWVGFNIATQTFAGYVFLVVAALIMTNWALAKHRRLKKLFDGKEGRPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TAIR 10
Match: AT3G55360.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 454.5 bits (1168), Expect = 6.0e-128
Identity = 217/310 (70.00%), Postives = 250/310 (80.65%), Query Frame = 0


Query: 1   MKVSVVSRSGREVVDGGIQLSDSASVADLQDAIYNRTKKFYPARQRLTLPVQPGSKERPT 60
           MKV+VVSRSGREV+   + L DSA+VADLQ+A + R KKFYP+RQRLTLPV PGSK++P 
Sbjct: 1   MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPV 60

Query: 61  --------REYCSNNSDNITVVFKDLGPQVSYRTLFFFEYLGPLILYPIFYYFPVYQFFG 120
                   +EYC  N++++TVVFKDLG QVSYRTLFFFEYLGPL++YP+FYYFPVY+F G
Sbjct: 61  VLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG 120

Query: 121 YKAERVIHPVQTYAMYYWCFHYFKRIMETFFV------------------------SYIA 180
           Y  + VIHPVQTYAMYYWCFHYFKRI+ETFFV                        +YIA
Sbjct: 121 YGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIA 180

Query: 181 YYVNHPLYSPVGDLQMKIGFAFGLLCQVCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIV 240
           YYVNHPLY+PV DLQMKIGF FGL+CQV NFYCHILL+ LR P G GGYQIP+GFLFNIV
Sbjct: 181 YYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIV 240

Query: 241 TCANYTTEIYQWVGFNIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYP 279
           TCANYTTEIYQW+GFNIATQT+AGY+FL VA LIMTNWAL KH RL+K+FDGK+G+P+YP
Sbjct: 241 TCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYP 300

[Close]

BLAST of Cp4.1LG01g23900 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 2.1e-08
Identity = 34/103 (33.01%), Postives = 51/103 (49.51%), Query Frame = 0


Query: 176 VCNFYCHILLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGFNIATQTVAGYIF 235
           V N Y H+LL KLR  +G   Y+IPKG LF+I+ C +Y  EI  +  F + +QT+  + F
Sbjct: 171 VGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLISQTIYSFSF 230

Query: 236 LVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 279
            +   L +   + A        FD       +P+    L PF+
Sbjct: 231 AMGTMLYLIGRSYATRTWYLSKFDD------FPKHIKALIPFV 267

[Close]

BLAST of Cp4.1LG01g23900 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.7e-05
Identity = 33/115 (28.70%), Postives = 54/115 (46.96%), Query Frame = 0


Query: 168 FAFGLLCQVCNFYCHI----LLRKLRSPEGNGGYQIPKGFLFNIVTCANYTTEIYQWVGF 227
           F  G++  +   Y +I     L +L+  E  GGY IP+G  F +V+C NY  E  +W+G+
Sbjct: 153 FVIGMVVFITGMYINITSDRTLVRLKK-ENRGGYVIPRGGWFELVSCPNYFGEAIEWLGW 212

Query: 228 NIATQTVAGYIFLVVATLIMTNWALAKHRRLKKLFDGKEGRPRYPRRWVILPPFL 279
            + T + AG  F +     +   A A H+     F     +  YP+    + PF+
Sbjct: 213 AVMTWSWAGIGFFLYTCSNLFPRARASHKWYIAKF-----KEEYPKTRKAVIPFV 261

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g23900 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2U2	8.5e-127	70.00	Very-long-chain enoyl-CoA reductase OS=Arabidopsis thaliana OX=3702 GN=ECR PE=1 ...	[more]
Q55C17	1.3e-37	34.63	Very-long-chain enoyl-CoA reductase OS=Dictyostelium discoideum OX=44689 GN=gpsn...	[more]
Q9N5Y2	1.9e-33	33.70	Probable very-long-chain enoyl-CoA reductase art-1 OS=Caenorhabditis elegans OX=...	[more]
Q9NZ01	7.4e-30	32.90	Very-long-chain enoyl-CoA reductase OS=Homo sapiens OX=9606 GN=TECR PE=1 SV=1	[more]
Q3ZCD7	1.6e-29	32.90	Very-long-chain enoyl-CoA reductase OS=Bos taurus OX=9913 GN=TECR PE=2 SV=1	[more]

BLAST of Cp4.1LG01g23900 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514664.1	1.15e-209	100.00	very-long-chain enoyl-CoA reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022921854.1	6.19e-199	88.71	very-long-chain enoyl-CoA reductase-like [Cucurbita moschata]	[more]
KAG6602374.1	1.77e-198	88.39	Very-long-chain enoyl-CoA reductase, partial [Cucurbita argyrosperma subsp. soro...	[more]
XP_022990641.1	1.77e-198	88.71	very-long-chain enoyl-CoA reductase-like [Cucurbita maxima]	[more]
XP_038890322.1	1.56e-192	85.48	very-long-chain enoyl-CoA reductase [Benincasa hispida]	[more]

BLAST of Cp4.1LG01g23900 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E1Q3	3.00e-199	88.71	very-long-chain enoyl-CoA reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JNI3	8.59e-199	88.71	very-long-chain enoyl-CoA reductase-like OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A0A0KNT8	7.55e-193	86.13	S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G5170...	[more]
A0A5A7T2H3	3.07e-192	85.81	Very-long-chain enoyl-CoA reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]
A0A1S3C9G7	3.07e-192	85.81	very-long-chain enoyl-CoA reductase OS=Cucumis melo OX=3656 GN=LOC103498153 PE=3...	[more]

BLAST of Cp4.1LG01g23900 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55360.1	6.0e-128	70.00	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	2.1e-08	33.01	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G38050.1	1.7e-05	28.70	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 132..277 e-value: 5.1E-24 score: 85.0
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 167..248 score: 21.603628
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 1..144 coord: 143..278
None	No IPR available	PANTHER	PTHR10556:SF28	SC2	coord: 1..144 coord: 143..278
None	No IPR available	CDD	cd01801	Ubl_TECR_like	coord: 2..78 e-value: 4.60073E-16 score: 69.2375

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g23900.1	Cp4.1LG01g23900.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors