Cp4.1LG01g23260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g23260 vs. ExPASy Swiss-Prot
Match: F4K2A1 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS1 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.8e-166
Identity = 318/553 (57.50%), Postives = 386/553 (69.80%), Query Frame = 0

Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 182 DVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALF 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+                  
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASE------------------ 305

Query: 302 TLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSY 361
                  ++V L VV  L   LL G KLGL+GEHQY+NA LA++L+S WL + G   +  
Sbjct: 306 -------TEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPS 365

Query: 362 KEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWF 421
           + Q   LPE+FI+GL TAS +GRAQ+VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF
Sbjct: 366 RTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWF 425

Query: 422 CAAIK-HNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQP 481
             A+K  NQ G   H ++ +  SS+     + N+       Q+LLFNCMSVRDP LLL  
Sbjct: 426 SVAVKGDNQSGSSGHLVNGSAGSSH---DKWSNETCE----QILLFNCMSVRDPNLLLPH 485

Query: 482 LIKTCAN--------------NVFNKVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEK 541
           L   CA               +V++KVGT   L E + +VDLSWQF +++VWE+++QSE+
Sbjct: 486 LKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSER 545

Query: 542 GGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTG 596
            G+  G       ES+          NS VF+SLPMAIK LRD+V + + + R QVLVTG
Sbjct: 546 DGEKDG-------ESD---------GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTG 570

BLAST of Cp4.1LG01g23260 vs. ExPASy Swiss-Prot
Match: Q8W035 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS3 PE=1 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.4e-142
Identity = 286/550 (52.00%), Postives = 354/550 (64.36%), Query Frame = 0

Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF L+F Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVCGIASLGYDHTEL 242
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALFT 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L                  
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKL------------------ 252

Query: 303 LPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYK 362
                  +V L VV  LD       +LGL+GEHQYLNA LA+AL ST+L   G  + +  
Sbjct: 253 -------EVNLQVVEPLD----SSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGL 312

Query: 363 EQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC 422
           +Q+  LPE+FI GL+ A   GRA IVPD     E PE++V+YLDGAHSPESM  C+ WF 
Sbjct: 313 DQTNGLPEKFISGLSNAYLMGRAMIVPDS----ELPEEIVYYLDGAHSPESMEACAIWFS 372

Query: 423 AAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI 482
             IK NQE                          +K+S Q+LLFNCMSVRDP LLL  L 
Sbjct: 373 KQIKQNQE------------------------RNQKRSEQILLFNCMSVRDPSLLLPRLR 432

Query: 483 KTCAN--------------NVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGD 542
             C +              +V+N+VG+        E  +SWQF ++R+WE++ + E   +
Sbjct: 433 SKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSN 491

Query: 543 SQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLH 596
           S            K +  GK  E S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLH
Sbjct: 493 S------------KSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLH 491

BLAST of Cp4.1LG01g23260 vs. ExPASy Swiss-Prot
Match: F4J2K2 (Folylpolyglutamate synthase OS=Arabidopsis thaliana OX=3702 GN=FPGS2 PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.3e-129
Identity = 271/584 (46.40%), Postives = 357/584 (61.13%), Query Frame = 0

Query: 61  TAYEEALDVLSSLIVSRRRCDK-----SGDRFELMFEYVKMLDLEESMSKMKVIHVAGTK 120
           ++Y++A++ LS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 121 GKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKE 180
           GKGSTC F+E+ILR CGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 181 KTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVCGIASLGY 240
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV Q P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 241 DHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHD 300
           DH ++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L             
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNL------------- 320

Query: 301 YALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHS 360
                       +V L VV  L+P+ L G  LGL G+HQ +NA LA++LS  WL RTG+ 
Sbjct: 321 ------------EVPLEVVAPLEPKKLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNW 380

Query: 361 NLSYKEQS--GSLPEQFIEGLTTASFEGRAQIVPDRYND-------IESP-EDLVFYLDG 420
              +  +S    +P  F  GL TA   GRAQ+V D  +D       +E+P  DL+FYLDG
Sbjct: 381 KKIFPNESKETEIPVAFCRGLATARLHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDG 440

Query: 421 AHSPESMVVCSSWFCAAIKHNQ------EGILDHQLDDNGTSSNKLLQGYDNDEARKKSV 480
           AHSPESM  C  WF +A++ ++       G + H   + GT  N++            S 
Sbjct: 441 AHSPESMEACGRWFSSAVRGDKSLSTAVNGYMRH--GEYGTDLNRV------------SK 500

Query: 481 QVLLFNCMSVRDPQLLLQPLIKTCAN--------------NVFNKV--GTHGLAEANGEV 540
           Q+LLFNCM VRDPQ+LL  L+ TCA+              + +NKV  G   +       
Sbjct: 501 QILLFNCMEVRDPQVLLPKLVTTCASSGTHFSRALFVPSMSTYNKVISGASAIPSDTRRK 560

Query: 541 DLSWQFHVRRVWENIIQSEKGG-----DSQGIGTMVEEE---SEKPEVSGKC---CENSA 596
           DL+WQF ++R+WE  IQ    G        GI  +   +    + P+  G       +SA
Sbjct: 561 DLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSA 620

BLAST of Cp4.1LG01g23260 vs. ExPASy Swiss-Prot
Match: A6H751 (Folylpolyglutamate synthase, mitochondrial OS=Bos taurus OX=9913 GN=FPGS PE=2 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.1e-74
Identity = 215/619 (34.73%), Postives = 299/619 (48.30%), Query Frame = 0

Query: 47  VTMEEGMGA---LKYSRTAYEEALDVLSSL--------IVSRRRCDKSGDRFELMFEYVK 106
           V +  G+ A   L+     Y++A+  L++L        +V R+R D    + E M  Y+ 
Sbjct: 23  VAVRRGLSAWPVLQEPDMEYQDAVRTLNTLQTNANYLEMVKRKRGDPQ-TQLEAMKLYLA 82

Query: 107 MLDLE-ESMSKMKVIHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDG 166
              L+ E + ++ +IH+ GTKGKGSTC FTE ILR  G  TG F+SPHL+ +RER R++G
Sbjct: 83  RSGLQVEDLDQLNIIHITGTKGKGSTCAFTERILRSYGLKTGFFSSPHLVQVRERIRING 142

Query: 167 IDISEEKFLAYFWWCYNRLKEKTSED--VPMPHLFRFLALLAFKIFSDEQVDVAILEVGL 226
             IS E F  +FW  Y+RL+E   +   V MP  FRFL L+AF +F  E+VD+A+LEVG+
Sbjct: 143 QPISSELFTKHFWRLYHRLEETKDDSSCVSMPGYFRFLTLMAFHVFLQEKVDLAVLEVGI 202

Query: 227 GGTFDATN-VQAPIVCGIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAM 286
           GG +D TN ++ P+VCGI SLG DHT LLG+T+  IA +K GIFK G PAFTV QPDE +
Sbjct: 203 GGAYDCTNIIRKPVVCGITSLGIDHTGLLGDTMEKIAWQKGGIFKSGVPAFTVLQPDEPL 262

Query: 287 HVLRDKASQLDVCAKLFHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGE 346
            VLRD+A Q+        S P         Y+C     L  +    P L     LGLEGE
Sbjct: 263 AVLRDRAQQI--------SCP--------LYLCP---PLQALEEGGPPL----TLGLEGE 322

Query: 347 HQYLNARLAIALSSTWLHRTGHSNL-SYKEQSGSLPEQ------------FIEGLTTASF 406
           HQ  NA LA+ ++  WL + G+ +    K    SLP Q              +GL    +
Sbjct: 323 HQRSNAALALQVARCWLQQKGYQDAGELKASRPSLPGQLPLAPVFQPTPRMQQGLRHTEW 382

Query: 407 EGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDNG 466
            GR Q++            L +YLDGAH+  SM  C  WF  A+   +         D+G
Sbjct: 383 PGRTQLL--------RRGPLTWYLDGAHTTSSMQACVRWFRQALHRCER-------PDSG 442

Query: 467 TSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTC-------ANNVFNKVG 526
           +                  V+VLLFN    RD   LL+ L++ C         N+     
Sbjct: 443 S-----------------EVRVLLFNSTGDRDSAALLK-LLQPCQFDYAIFCPNLAEMAS 502

Query: 527 THGLAEANGEVD--------LSWQFHVRRVWENIIQ----SEKGGDSQGIGTMVEEESEK 586
           T    + N  V         L+ Q H  R+ E  +     S  G +  G  +++      
Sbjct: 503 TDNADQQNFTVTLDQVLLRCLAHQQHWSRLHEEQVSPDPWSTPGQEQDGPASLL----LA 562

Query: 587 PEVSGKCCENSAVFSSLPMAIKWL---RDSVLQQ--------------------NRSIRL 596
           P        +S VFS +  A++W+   RD + Q                       +  +
Sbjct: 563 PHPPHTHSASSLVFSCISHALQWITQGRDPLFQPPSPPRGLLAHPVADSGATVLQEAADI 580

BLAST of Cp4.1LG01g23260 vs. ExPASy Swiss-Prot
Match: Q05932 (Folylpolyglutamate synthase, mitochondrial OS=Homo sapiens OX=9606 GN=FPGS PE=1 SV=3)

HSP 1 Score: 275.4 bits (703), Expect = 1.5e-72
Identity = 207/595 (34.79%), Postives = 284/595 (47.73%), Query Frame = 0

Query: 63  YEEALDVLSSL--------IVSRRRCDKSGDRFELMFEYVKMLDLE-ESMSKMKVIHVAG 122
           Y++A+ +L++L         V R+R D    + E M  Y+    L+ E + ++ +IHV G
Sbjct: 45  YQDAVRMLNTLQTNAGYLEQVKRQRGDPQ-TQLEAMELYLARSGLQVEDLDRLNIIHVTG 104

Query: 123 TKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRL 182
           TKGKGSTC FTE ILR  G  TG F+SPHL+ +RER R++G  IS E F  YFW  Y+RL
Sbjct: 105 TKGKGSTCAFTECILRSYGLKTGFFSSPHLVQVRERIRINGQPISPELFTKYFWRLYHRL 164

Query: 183 KE-KTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQAPIVCGIAS 242
           +E K    V MP  FRFL L+AF +F  E+VD+A++EVG+GG +D TN ++ P+VCG++S
Sbjct: 165 EETKDGSCVSMPPYFRFLTLMAFHVFLQEKVDLAVVEVGIGGAYDCTNIIRKPVVCGVSS 224

Query: 243 LGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSL 302
           LG DHT LLG+T+  IA +K GIFK+G PAFTV QP+  + VLRD+A Q+        S 
Sbjct: 225 LGIDHTSLLGDTVEKIAWQKGGIFKQGVPAFTVLQPEGPLAVLRDRAQQI--------SC 284

Query: 303 PHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWL--- 362
           P         Y+C     L  +    P L     LGLEGEHQ  NA LA+ L+  WL   
Sbjct: 285 P--------LYLCP---MLEALEEGGPPL----TLGLEGEHQRSNAALALQLAHCWLQRQ 344

Query: 363 --HRTGHSNLSYKEQSGSLP--------EQFIEGLTTASFEGRAQIVPDRYNDIESPEDL 422
             H  G    S       LP             GL    + GR Q++            L
Sbjct: 345 DRHGAGEPKASRPGLLWQLPLAPVFQPTSHMRLGLRNTEWPGRTQVL--------RRGPL 404

Query: 423 VFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSV 482
            +YLDGAH+  S   C  WF                        + LQG +      + V
Sbjct: 405 TWYLDGAHTASSAQACVRWF-----------------------RQALQGRERPSGGPE-V 464

Query: 483 QVLLFNCMSVRDPQLLLQPLIKTC-------ANNVFNKVGTHGLAEANGEVDLSWQ---- 542
           +VLLFN    RDP  LL+ L++ C         N+     T    + N  V L       
Sbjct: 465 RVLLFNATGDRDPAALLK-LLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRC 524

Query: 543 FHVRRVWENIIQSEKGGDSQGIGTMVEEESEK----PEVSGKCCENSAVFSSLPMAIKWL 596
              ++ W ++ + +   D     +     S      P     C  +S VFS +  A++W+
Sbjct: 525 LEHQQHWNHLDEEQASPDLWSAPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWI 582

BLAST of Cp4.1LG01g23260 vs. NCBI nr
Match: KAG6602447.1 (Folylpolyglutamate synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 559/610 (91.64%), Postives = 564/610 (92.46%), Query Frame = 0

Query: 1   MSLSFSSCPLPVFGTRPRLLRRSQKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR 60
           M LSFSS PLPVFGTRPRLLRRS+KEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR
Sbjct: 1   MPLSFSSRPLPVFGTRPRLLRRSRKEEIGEKTRLNPQDIGEAGGHLVTMEEGMGALKYSR 60

Query: 61  TAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGST 120
           TAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKVIHVAGTKGKGST
Sbjct: 61  TAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKVIHVAGTKGKGST 120

Query: 121 CTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 180
           CTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED
Sbjct: 121 CTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 180

Query: 181 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVCGIASLGYDHTEL 240
           VPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNV QAPIVCGIASLGYDHTEL
Sbjct: 181 VPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVCGIASLGYDHTEL 240

Query: 241 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALFT 300
           LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV                
Sbjct: 241 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---------------- 300

Query: 301 LPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYK 360
                    QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYK
Sbjct: 301 ---------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYK 360

Query: 361 EQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC 420
           EQSGSLPE FIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC
Sbjct: 361 EQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC 420

Query: 421 AAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI 480
           AAIKHNQEGILDHQLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI
Sbjct: 421 AAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI 480

Query: 481 KTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGD 540
           KTCANN              +FNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGD
Sbjct: 481 KTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGD 540

Query: 541 SQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLH 595
           SQGIG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLH
Sbjct: 541 SQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLH 585

BLAST of Cp4.1LG01g23260 vs. NCBI nr
Match: XP_023533507.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023 bits (2644), Expect = 0.0
Identity = 522/562 (92.88%), Postives = 522/562 (92.88%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTCANN              VFNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 537

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Cp4.1LG01g23260 vs. NCBI nr
Match: XP_022961882.1 (folylpolyglutamate synthase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/562 (91.46%), Postives = 518/562 (92.17%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTC NN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCTNNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKGGDSQGIG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 537

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Cp4.1LG01g23260 vs. NCBI nr
Match: KAG7033130.1 (Folylpolyglutamate synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 513/562 (91.28%), Postives = 517/562 (91.99%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDG DISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGFDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPE FIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEHFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTCANN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKGGDSQGIG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 537

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Cp4.1LG01g23260 vs. NCBI nr
Match: XP_023533514.1 (folylpolyglutamate synthase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1006 bits (2601), Expect = 0.0
Identity = 517/562 (91.99%), Postives = 517/562 (91.99%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTCANN              VFNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCANNGVHFKKAVFVPNTSVFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKG     IGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKG-----IGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 532

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 532

BLAST of Cp4.1LG01g23260 vs. ExPASy TrEMBL
Match: A0A6J1HBB1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 1009 bits (2609), Expect = 0.0
Identity = 514/562 (91.46%), Postives = 518/562 (92.17%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTC NN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCTNNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKGGDSQGIG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 537

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Cp4.1LG01g23260 vs. ExPASy TrEMBL
Match: A0A6J1JQH1 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 1000 bits (2585), Expect = 0.0
Identity = 512/562 (91.10%), Postives = 516/562 (91.81%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFELMFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTG SNLSYKEQSGSLPEQFIEGLTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHS
Sbjct: 301 LHRTGRSNLSYKEQSGSLPEQFIEGLTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGIL+HQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILNHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTCANN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCANNGVHFKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKGGDSQGIG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKGGDSQGIGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 537

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 537

BLAST of Cp4.1LG01g23260 vs. ExPASy TrEMBL
Match: A0A6J1HDI1 (Folylpolyglutamate synthase OS=Cucurbita moschata OX=3662 GN=LOC111462519 PE=3 SV=1)

HSP 1 Score: 993 bits (2566), Expect = 0.0
Identity = 509/562 (90.57%), Postives = 513/562 (91.28%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFEL+FEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELLFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLID+RERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDVRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIF+DEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFADEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS
Sbjct: 301 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGILDHQLDD GTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILDHQLDDTGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTC NN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCTNNGVHFKKAVFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKG     IG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKG-----IGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 532

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 532

BLAST of Cp4.1LG01g23260 vs. ExPASy TrEMBL
Match: A0A6J1JTP4 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 985 bits (2546), Expect = 0.0
Identity = 512/590 (86.78%), Postives = 516/590 (87.46%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFELMFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFK----------------------------RGA 288
           GIASLGYDHTELLGNTLGAIASEKAGIFK                            RGA
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKVCLKKLSLKHERLGIISSFCFHPFTLVQRGA 240

Query: 289 PAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALFTLPYICTSQVQLTVVPSLDPE 348
           PAFTV QPDEAMHVLRDKASQLDV                         QLTVVPSLDPE
Sbjct: 241 PAFTVFQPDEAMHVLRDKASQLDV-------------------------QLTVVPSLDPE 300

Query: 349 LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKEQSGSLPEQFIEGLTTASFE 408
           LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTG SNLSYKEQSGSLPEQFIEGLTTA FE
Sbjct: 301 LLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGRSNLSYKEQSGSLPEQFIEGLTTARFE 360

Query: 409 GRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILDHQLDDNGT 468
           GRAQIVPDRYNDIE+PEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGIL+HQLDDNGT
Sbjct: 361 GRAQIVPDRYNDIETPEDLVFYLDGAHSPESMVVCSSWFCAAIKHNQEGILNHQLDDNGT 420

Query: 469 SSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANN-------------- 528
           SSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANN              
Sbjct: 421 SSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIKTCANNGVHFKKALFVPNTS 480

Query: 529 VFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGTMVEEESEKPEVSGK 588
           +FNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIG MVEEESEKPEVSGK
Sbjct: 481 LFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDSQGIGAMVEEESEKPEVSGK 540

Query: 589 CCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 595
            CENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 SCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHLVGDVLKLIK 565

BLAST of Cp4.1LG01g23260 vs. ExPASy TrEMBL
Match: A0A6J1JNC2 (Folylpolyglutamate synthase OS=Cucurbita maxima OX=3661 GN=LOC111487415 PE=3 SV=1)

HSP 1 Score: 983 bits (2542), Expect = 0.0
Identity = 507/562 (90.21%), Postives = 511/562 (90.93%), Query Frame = 0

Query: 49  MEEGMGALKYSRTAYEEALDVLSSLIVSRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 108
           MEEGMGALKYSRTAYEEALDVL SLIV+RRRCDKSGDRFELMFEYVKMLDLEESMSKMKV
Sbjct: 1   MEEGMGALKYSRTAYEEALDVLFSLIVNRRRCDKSGDRFELMFEYVKMLDLEESMSKMKV 60

Query: 109 IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 168
           IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW
Sbjct: 61  IHVAGTKGKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWW 120

Query: 169 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVC 228
           CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV QAPIVC
Sbjct: 121 CYNRLKEKTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVVQAPIVC 180

Query: 229 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKL 288
           GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTV QPDEAMHVLRDKASQLDV    
Sbjct: 181 GIASLGYDHTELLGNTLGAIASEKAGIFKRGAPAFTVFQPDEAMHVLRDKASQLDV---- 240

Query: 289 FHSLPHDYALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 348
                                QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW
Sbjct: 241 ---------------------QLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTW 300

Query: 349 LHRTGHSNLSYKEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHS 408
           LHRTG SNLSYKEQSGSLPEQFIEGLTTA FEGRAQIVPDRYNDIE+PEDLVFYLDGAHS
Sbjct: 301 LHRTGRSNLSYKEQSGSLPEQFIEGLTTARFEGRAQIVPDRYNDIETPEDLVFYLDGAHS 360

Query: 409 PESMVVCSSWFCAAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 468
           PESMVVCSSWFCAAIKHNQEGIL+HQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS
Sbjct: 361 PESMVVCSSWFCAAIKHNQEGILNHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMS 420

Query: 469 VRDPQLLLQPLIKTCANN--------------VFNKVGTHGLAEANGEVDLSWQFHVRRV 528
           VRDPQLLLQPLIKTCANN              +FNKVGTHGLAEANGEVDLSWQFHVRRV
Sbjct: 421 VRDPQLLLQPLIKTCANNGVHFKKALFVPNTSLFNKVGTHGLAEANGEVDLSWQFHVRRV 480

Query: 529 WENIIQSEKGGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRS 588
           WENIIQSEKG     IG MVEEESEKPEVSGK CENSAVFSSLPMAIKWLRDSVLQQNRS
Sbjct: 481 WENIIQSEKG-----IGAMVEEESEKPEVSGKSCENSAVFSSLPMAIKWLRDSVLQQNRS 532

Query: 589 IRLQVLVTGSLHLVGDVLKLIK 595
           IRLQVLVTGSLHLVGDVLKLIK
Sbjct: 541 IRLQVLVTGSLHLVGDVLKLIK 532

BLAST of Cp4.1LG01g23260 vs. TAIR 10
Match: AT5G05980.1 (DHFS-FPGS homolog B )

HSP 1 Score: 587.4 bits (1513), Expect = 1.3e-167
Identity = 318/553 (57.50%), Postives = 386/553 (69.80%), Query Frame = 0

Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 66  SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 125

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 126 TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 185

Query: 182 DVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 186 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 245

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALF 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+                  
Sbjct: 246 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASE------------------ 305

Query: 302 TLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSY 361
                  ++V L VV  L   LL G KLGL+GEHQY+NA LA++L+S WL + G   +  
Sbjct: 306 -------TEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPS 365

Query: 362 KEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWF 421
           + Q   LPE+FI+GL TAS +GRAQ+VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF
Sbjct: 366 RTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWF 425

Query: 422 CAAIK-HNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQP 481
             A+K  NQ G   H ++ +  SS+     + N+       Q+LLFNCMSVRDP LLL  
Sbjct: 426 SVAVKGDNQSGSSGHLVNGSAGSSH---DKWSNETCE----QILLFNCMSVRDPNLLLPH 485

Query: 482 LIKTCAN--------------NVFNKVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEK 541
           L   CA               +V++KVGT   L E + +VDLSWQF +++VWE+++QSE+
Sbjct: 486 LKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSER 545

Query: 542 GGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTG 596
            G+  G       ES+          NS VF+SLPMAIK LRD+V + + + R QVLVTG
Sbjct: 546 DGEKDG-------ESD---------GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTG 570

BLAST of Cp4.1LG01g23260 vs. TAIR 10
Match: AT5G05980.2 (DHFS-FPGS homolog B )

HSP 1 Score: 587.4 bits (1513), Expect = 1.3e-167
Identity = 318/553 (57.50%), Postives = 386/553 (69.80%), Query Frame = 0

Query: 62  AYEEALDVLSSLIVSRRRCDKS--GDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGS 121
           +YEEAL  LSSLI  R R DKS  GDRFEL+F+Y+K+LDLEE + KM VIHVAGTKGKGS
Sbjct: 8   SYEEALAALSSLITKRSRADKSNKGDRFELVFDYLKLLDLEEDILKMNVIHVAGTKGKGS 67

Query: 122 TCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSE 181
           TCTFTESI+R  GF TGLFTSPHLID+RERFRLDG+DISEEKFL YFWWCYNRLKE+T+E
Sbjct: 68  TCTFTESIIRNYGFRTGLFTSPHLIDVRERFRLDGVDISEEKFLGYFWWCYNRLKERTNE 127

Query: 182 DVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATN-VQAPIVCGIASLGYDHTE 241
           ++PMP  FRFLALLAFKIF+ E+VD AILEVGLGG FDATN VQ P+VCGI+SLGYDH E
Sbjct: 128 EIPMPTYFRFLALLAFKIFAAEEVDAAILEVGLGGKFDATNAVQKPVVCGISSLGYDHME 187

Query: 242 LLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALF 301
           +LG+TLG IA EKAGIFK G PAFTV QPDEAM VL +KAS+                  
Sbjct: 188 ILGDTLGKIAGEKAGIFKLGVPAFTVPQPDEAMRVLEEKASE------------------ 247

Query: 302 TLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSY 361
                  ++V L VV  L   LL G KLGL+GEHQY+NA LA++L+S WL + G   +  
Sbjct: 248 -------TEVNLEVVQPLTARLLSGQKLGLDGEHQYVNAGLAVSLASIWLQQIGKLEVPS 307

Query: 362 KEQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWF 421
           + Q   LPE+FI+GL TAS +GRAQ+VPD+Y +  +  DLVFYLDGAHSPESM  C+ WF
Sbjct: 308 RTQMSILPEKFIKGLATASLQGRAQVVPDQYTESRTSGDLVFYLDGAHSPESMEACAKWF 367

Query: 422 CAAIK-HNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQP 481
             A+K  NQ G   H ++ +  SS+     + N+       Q+LLFNCMSVRDP LLL  
Sbjct: 368 SVAVKGDNQSGSSGHLVNGSAGSSH---DKWSNETCE----QILLFNCMSVRDPNLLLPH 427

Query: 482 LIKTCAN--------------NVFNKVGTHG-LAEANGEVDLSWQFHVRRVWENIIQSEK 541
           L   CA               +V++KVGT   L E + +VDLSWQF +++VWE+++QSE+
Sbjct: 428 LKNMCAKYGVNFKKALFVPNMSVYHKVGTAADLPENDPQVDLSWQFTLQKVWESLVQSER 487

Query: 542 GGDSQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTG 596
            G+  G       ES+          NS VF+SLPMAIK LRD+V + + + R QVLVTG
Sbjct: 488 DGEKDG-------ESD---------GNSEVFTSLPMAIKCLRDTVHESSSATRFQVLVTG 512

BLAST of Cp4.1LG01g23260 vs. TAIR 10
Match: AT3G55630.2 (DHFS-FPGS homolog D )

HSP 1 Score: 512.3 bits (1318), Expect = 5.2e-145
Identity = 285/549 (51.91%), Postives = 354/549 (64.48%), Query Frame = 0

Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF L+F Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNVQAPIVCGIASLGYDHTELL 242
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATN+Q P+VCGI+SLGYDH E+L
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNIQKPVVCGISSLGYDHMEIL 192

Query: 243 GNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALFTL 302
           G TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L                   
Sbjct: 193 GYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKL------------------- 252

Query: 303 PYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYKE 362
                 +V L VV  LD       +LGL+GEHQYLNA LA+AL ST+L   G  + +  +
Sbjct: 253 ------EVNLQVVEPLD----SSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGLD 312

Query: 363 QSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFCA 422
           Q+  LPE+FI GL+ A   GRA IVPD     E PE++V+YLDGAHSPESM  C+ WF  
Sbjct: 313 QTNGLPEKFISGLSNAYLMGRAMIVPDS----ELPEEIVYYLDGAHSPESMEACAIWFSK 372

Query: 423 AIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLIK 482
            IK NQE                          +K+S Q+LLFNCMSVRDP LLL  L  
Sbjct: 373 QIKQNQE------------------------RNQKRSEQILLFNCMSVRDPSLLLPRLRS 432

Query: 483 TCAN--------------NVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGDS 542
            C +              +V+N+VG+        E  +SWQF ++R+WE++ + E   +S
Sbjct: 433 KCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSNS 490

Query: 543 QGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLHL 596
                       K +  GK  E S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLHL
Sbjct: 493 ------------KSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLHL 490

BLAST of Cp4.1LG01g23260 vs. TAIR 10
Match: AT3G55630.3 (DHFS-FPGS homolog D )

HSP 1 Score: 508.1 bits (1307), Expect = 9.8e-144
Identity = 286/550 (52.00%), Postives = 354/550 (64.36%), Query Frame = 0

Query: 63  YEEALDVLSSLIVSRRR--CDKSGDRFELMFEYVKMLDLEESMSKMKVIHVAGTKGKGST 122
           Y+  LD LSSLI  R R   +    RF L+F Y+K+L+LE+++S+MK+IHVAGTKGKGST
Sbjct: 13  YQNTLDALSSLITKRGRLASNNQSHRFRLLFHYLKVLELEDAVSQMKIIHVAGTKGKGST 72

Query: 123 CTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKEKTSED 182
           CTF ESILR  G  TGLFTSPHLID+RERFRL+GI+IS+EKF+ YFW C+++LKEKTS +
Sbjct: 73  CTFAESILRCYGLRTGLFTSPHLIDVRERFRLNGIEISQEKFVNYFWCCFHKLKEKTSNE 132

Query: 183 VPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVCGIASLGYDHTEL 242
           VPMP  F FLALLAFKIF+ EQVDV ILEVGLGG FDATNV Q P+VCGI+SLGYDH E+
Sbjct: 133 VPMPTYFCFLALLAFKIFTTEQVDVVILEVGLGGRFDATNVIQKPVVCGISSLGYDHMEI 192

Query: 243 LGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHDYALFT 302
           LG TL  IA+EKAGIFK G PAFTV QPDEAM VL +KAS+L                  
Sbjct: 193 LGYTLAEIAAEKAGIFKSGVPAFTVAQPDEAMRVLNEKASKL------------------ 252

Query: 303 LPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHSNLSYK 362
                  +V L VV  LD       +LGL+GEHQYLNA LA+AL ST+L   G  + +  
Sbjct: 253 -------EVNLQVVEPLD----SSQRLGLQGEHQYLNAGLAVALCSTFLKEIGIEDKNGL 312

Query: 363 EQSGSLPEQFIEGLTTASFEGRAQIVPDRYNDIESPEDLVFYLDGAHSPESMVVCSSWFC 422
           +Q+  LPE+FI GL+ A   GRA IVPD     E PE++V+YLDGAHSPESM  C+ WF 
Sbjct: 313 DQTNGLPEKFISGLSNAYLMGRAMIVPDS----ELPEEIVYYLDGAHSPESMEACAIWFS 372

Query: 423 AAIKHNQEGILDHQLDDNGTSSNKLLQGYDNDEARKKSVQVLLFNCMSVRDPQLLLQPLI 482
             IK NQE                          +K+S Q+LLFNCMSVRDP LLL  L 
Sbjct: 373 KQIKQNQE------------------------RNQKRSEQILLFNCMSVRDPSLLLPRLR 432

Query: 483 KTCAN--------------NVFNKVGTHGLAEANGEVDLSWQFHVRRVWENIIQSEKGGD 542
             C +              +V+N+VG+        E  +SWQF ++R+WE++ + E   +
Sbjct: 433 SKCIDQGVDFKRAVFVPNVSVYNQVGSSTNVGTRVE-SMSWQFGLQRIWESLARGEAKSN 491

Query: 543 SQGIGTMVEEESEKPEVSGKCCENSAVFSSLPMAIKWLRDSVLQQNRSIRLQVLVTGSLH 596
           S            K +  GK  E S VFSSLP+A+ WLRD+  +Q++ +R QVLVTGSLH
Sbjct: 493 S------------KSDSKGKEEEKSFVFSSLPVAVDWLRDNA-RQSKQVRFQVLVTGSLH 491

BLAST of Cp4.1LG01g23260 vs. TAIR 10
Match: AT3G10160.1 (DHFS-FPGS homolog C )

HSP 1 Score: 464.9 bits (1195), Expect = 9.5e-131
Identity = 271/584 (46.40%), Postives = 357/584 (61.13%), Query Frame = 0

Query: 61  TAYEEALDVLSSLIVSRRRCDK-----SGDRFELMFEYVKMLDLEESMSKMKVIHVAGTK 120
           ++Y++A++ LS+LI  R R D+     + D+ E +  Y+K+LDLE+ + ++KVIHVAGTK
Sbjct: 81  SSYDDAMEALSTLISRRNRGDRTPTKGNRDKLEQVVTYLKILDLEDKIKELKVIHVAGTK 140

Query: 121 GKGSTCTFTESILRKCGFHTGLFTSPHLIDIRERFRLDGIDISEEKFLAYFWWCYNRLKE 180
           GKGSTC F+E+ILR CGF TG+FTSPHLID+RERFR+DG+DISEEKFL YFW C+  LKE
Sbjct: 141 GKGSTCVFSEAILRNCGFRTGMFTSPHLIDVRERFRIDGLDISEEKFLQYFWECWKLLKE 200

Query: 181 KTSEDVPMPHLFRFLALLAFKIFSDEQVDVAILEVGLGGTFDATNV-QAPIVCGIASLGY 240
           K  + + MP LF+FL +LAFKIF  E+VDVA++EVGLGG  D+TNV Q P+VCGIASLG 
Sbjct: 201 KAVDGLTMPPLFQFLTVLAFKIFVCEKVDVAVIEVGLGGKLDSTNVIQKPVVCGIASLGM 260

Query: 241 DHTELLGNTLGAIASEKAGIFKRGAPAFTVCQPDEAMHVLRDKASQLDVCAKLFHSLPHD 300
           DH ++LGNTL  IA  KAGIFK   PAFTV Q  EAM VL+  A+ L             
Sbjct: 261 DHMDILGNTLADIAFHKAGIFKPQIPAFTVPQLSEAMDVLQKTANNL------------- 320

Query: 301 YALFTLPYICTSQVQLTVVPSLDPELLQGHKLGLEGEHQYLNARLAIALSSTWLHRTGHS 360
                       +V L VV  L+P+ L G  LGL G+HQ +NA LA++LS  WL RTG+ 
Sbjct: 321 ------------EVPLEVVAPLEPKKLDGVTLGLSGDHQLVNAGLAVSLSRCWLQRTGNW 380

Query: 361 NLSYKEQS--GSLPEQFIEGLTTASFEGRAQIVPDRYND-------IESP-EDLVFYLDG 420
              +  +S    +P  F  GL TA   GRAQ+V D  +D       +E+P  DL+FYLDG
Sbjct: 381 KKIFPNESKETEIPVAFCRGLATARLHGRAQVVHDVVSDPQDSSDSMETPCGDLIFYLDG 440

Query: 421 AHSPESMVVCSSWFCAAIKHNQ------EGILDHQLDDNGTSSNKLLQGYDNDEARKKSV 480
           AHSPESM  C  WF +A++ ++       G + H   + GT  N++            S 
Sbjct: 441 AHSPESMEACGRWFSSAVRGDKSLSTAVNGYMRH--GEYGTDLNRV------------SK 500

Query: 481 QVLLFNCMSVRDPQLLLQPLIKTCAN--------------NVFNKV--GTHGLAEANGEV 540
           Q+LLFNCM VRDPQ+LL  L+ TCA+              + +NKV  G   +       
Sbjct: 501 QILLFNCMEVRDPQVLLPKLVTTCASSGTHFSRALFVPSMSTYNKVISGASAIPSDTRRK 560

Query: 541 DLSWQFHVRRVWENIIQSEKGG-----DSQGIGTMVEEE---SEKPEVSGKC---CENSA 596
           DL+WQF ++R+WE  IQ    G        GI  +   +    + P+  G       +SA
Sbjct: 561 DLTWQFRLQRLWEKSIQGTDAGLDHTLKPDGITALPPHDFLCGDAPQCGGPAGTPVTSSA 620