Cp4.1LG01g22450 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG01g22450
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Cytochrome P450
Location	Cp4.1LG01: 20210315 .. 20212766 (+)
RNA-Seq Expression	Cp4.1LG01g22450
Synteny	Cp4.1LG01g22450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTTGGCCTTAGCGATTCTAGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTGCTTCGATGGAATGAAGTTCGATACAGAAGAAAAGGGTTGCCCCCTGGTACAATGGGATGGCCTTTTTTTGGTGAGACCACTGAGTTTCTCAAACAAGGTCCTAATTTTATGAAATCCCAAAGAGCAAGGNGCTTTTTAATCATTGCTCTGTTTTTTTTTTTTTTGTAAAGGTATGGAAGCATTTTCAAGTCCCATATTTTGGGGTGTCCAACGATTGTTTCGATGGATCCTGAGGTTAATCGATATGTTTTGATGAATGAATCAAAAGGGCTTGTCCCTGGTTACCCTCAGTCCATGCTTGACATTCTTGGCAAATGCAATATTGCCGCCGTCCATGGCGGTGCTCACAAGGTCATGAGAGCTGCTCTGCTCTCTGTTGTCAGTCCCACCATGATTAGAGATCGTCTTCTTCCTAAAGTTGATGACTTTATGCGATCCCATTTGAGGAATTGGGATCAACAAATCATTGACATCCAAGAGAAGACGAAAGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGTGTGTGTTCAGATGGCTCTACGGTCTTCTATGAAGCAGATTGCCGGAATCGAGTCCGGTCCGTTATCGGAATCTTTCATCCCAGAGTTCTTTAAGCTTGTTCTCGGCACGCTCTCGCTCCCGATCGATCTTCCCGGCACGAACTACCGCCGAGGAATTCAGGTGAAGCATAGGTTTCTATACATTGTAAAAGATGTCTGTGTCATCTCCATCTCTTATTGGGTTTAAGCTTTTGGAGGGTTGTGATTTTCAGGCGAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACACAAAAGGATGACATGCTTGGATTCTTCATGAGTGAAGAGAACAAATATAAGCTAAACGATGAAGAGGTTATTGATCAAATCATCACAGTTTTGTACTCTGGCTATGAAACTGTTTCGACAACATCAATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTAAGAGTAAGAGACCAAAAGAGCTTTACAACCTTTTGAGTTGGAAGTTTGAAAAGGCAATTCTCTTTTACTTTTTTGTAAGTTAAAAGAGTTGATAAGTGCAGGAAGAGCATTTTGCAATCAGAGAAAAGAAAAGGGCAGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTACTTACTGTCGTCTTTTTAACTGTTCTTTAGAGGAAAAGAAAAAGACACCAACTTTACTCTGTTTTGAATTGTTTAGGTGATATTCGAAACATCGAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACGAAGGACATGGAGCTAAATGGTATATATGTTTTTTTTTTCTTTAGTTTTTGTTTTGTTTTGTGTTATTTAGGGGCTCAAGATCCTCTTTTGCAGTTACCAATTCATCTCCTTTTTTTTCACTTTGGGCCACTTTCTCTCTCTCTGTTTCTCCTCTGTTTCTCCTCTGTTTTGTGGGTCAGGCTTAGATAAGGGTCATACAGAATGACAGATCTTTTTCACCTCCTCATTACAACAATGTTCTGAGAGATCAAACCAAAAAGTTGGGCTAAAAAGTCAAAATCTTCTCACTTTTTTTCTTTTTTGTTTGGTGTTTTTTACAGGGTTTCTCATTCCAAAAGGATGGAGGATTTATGTATACACCAGAGAGATTAACTATGACGCTTTTCTCTATTCTGAACCTTTAACCTTCAACCCATGGAGATGGCTGGTGGGTCTTCTTCCCTCTCTCTCTCTCTCTTACTTACCCTCCATTGCCAAAATGACGAATTTGCCCTTGTGTGACATTCTCAGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTCGGAGGTGGAACCAGACATTGCCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGGTACCTTTCTTTTTTCCTGTGTATTGTTTGGCTAAATGGAACGAACAGAAGCTGAGTTCAAGATTCAAACTTTTATTTCTTTTGCAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAGTTTCCAAGGGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCATACTGACTGAACTAATTAATAATATTATAAAAAAAAGGGAGTTTGATGTACAGTGGGAGATGATGATATATGGGTAATGTCTATGTTAAAGAGAGATTGATGAAGGGATGATGATGTTTATAACATTCTAACACCTTAGTGACAAAAGGAATGAAATTGAATTTTCTCAACTGTTTGCATCTCTCTGTTTCCATTGCAAAGGACACTACT

mRNA sequence

ATGGCGTTGGCCTTAGCGATTCTAGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTGCTTCGATGGAATGAAGTTCGATACAGAAGAAAAGGGTTGCCCCCTGGTACAATGGGATGGCCTTTTTTTGGTGAGACCACTGAGTTTCTCAAACAAGGGCTTGTCCCTGGTTACCCTCAGTCCATGCTTGACATTCTTGGCAAATGCAATATTGCCGCCGTCCATGGCGGTGCTCACAAGGCGAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACACAAAAGGATGACATGCTTGGATTCTTCATGAGTGAAGAGAACAAATATAAGCTAAACGATGAAGAGGTTATTGATCAAATCATCACAGTTTTGTACTCTGGCTATGAAACTGTTTCGACAACATCAATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTAAGAGAAGAGCATTTTGCAATCAGAGAAAAGAAAAGGGCAGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTGATATTCGAAACATCGAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACGAAGGACATGGAGCTAAATGGGTTTCTCATTCCAAAAGGATGGAGGATTTATGTATACACCAGAGAGATTAACTATGACGCTTTTCTCTATTCTGAACCTTTAACCTTCAACCCATGGAGATGGCTGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTCGGAGGTGGAACCAGACATTGCCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAGTTTCCAAGGGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCATACTGACTGAACTAATTAATAATATTATAAAAAAAAGGGAGTTTGATGTACAGTGGGAGATGATGATATATGGGTAATGTCTATGTTAAAGAGAGATTGATGAAGGGATGATGATGTTTATAACATTCTAACACCTTAGTGACAAAAGGAATGAAATTGAATTTTCTCAACTGTTTGCATCTCTCTGTTTCCATTGCAAAGGACACTACT

Coding sequence (CDS)

ATGGCGTTGGCCTTAGCGATTCTAGCTCTGTTCTTGCTCCTCTGTTTTTGCTCTGCTTTGCTTCGATGGAATGAAGTTCGATACAGAAGAAAAGGGTTGCCCCCTGGTACAATGGGATGGCCTTTTTTTGGTGAGACCACTGAGTTTCTCAAACAAGGGCTTGTCCCTGGTTACCCTCAGTCCATGCTTGACATTCTTGGCAAATGCAATATTGCCGCCGTCCATGGCGGTGCTCACAAGGCGAGGAAGAACATCATAAAGCTGTTGCAGCAGCTGATAAACGAGAGAAGAGCCTCAGAGACACAAAAGGATGACATGCTTGGATTCTTCATGAGTGAAGAGAACAAATATAAGCTAAACGATGAAGAGGTTATTGATCAAATCATCACAGTTTTGTACTCTGGCTATGAAACTGTTTCGACAACATCAATGATGGCTGTCAAGTTCCTCCATGATCATCCCAAAGTTCTTCAGCAACTAAGAGAAGAGCATTTTGCAATCAGAGAAAAGAAAAGGGCAGAGGACCCCATTGATTGGGATGATCTTAAAGCCATGAAGTTCACTCGTGCGGTGATATTCGAAACATCGAGGCTGGCCACAATTGTTAATGGGGTTTTGAGGAAAACTACGAAGGACATGGAGCTAAATGGGTTTCTCATTCCAAAAGGATGGAGGATTTATGTATACACCAGAGAGATTAACTATGACGCTTTTCTCTATTCTGAACCTTTAACCTTCAACCCATGGAGATGGCTGGACAAAAACTTGGAGTCCCATAATTACTTCTTCATCTTCGGAGGTGGAACCAGACATTGCCCTGGCAAAGAACTGGGAATTGCAGAGATTTCTACTTTTTTACATTACTTTGTAACTAAATACAGATGGGAAGAAGTTGGAGGAGATAAGCTGTTGAAGTTTCCAAGGGTGGAAGCTCCAAGTGGGTTCCACATTAGGGTTTCATCATACTGA

Protein sequence

MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGLVPGYPQSMLDILGKCNIAAVHGGAHKARKNIIKLLQQLINERRASETQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDAFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTRHCPGKELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY

Homology

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot
Match: Q69F95 (Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2)

HSP 1 Score: 471.9 bits (1213), Expect = 6.0e-132
Identity = 256/464 (55.17%), Postives = 290/464 (62.50%), Query Frame = 0


Query: 1   MALALAIL-ALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ------- 60
           MAL +AIL  L LLLCFCSALL+WNEVR+RRKGLPPG MGWP FGETTEFLKQ       
Sbjct: 1   MALFMAILGVLVLLLCFCSALLKWNEVRFRRKGLPPGAMGWPVFGETTEFLKQGPNFMKN 60

Query: 61  ------------------------------------GLVPGYPQSMLDILGKCNIAAVHG 120
                                               GLVPGYPQSMLDILG  NIAAVHG
Sbjct: 61  KRARYGSFFKSHILGCPTIVSMDPELNRFILMNEAKGLVPGYPQSMLDILGTRNIAAVHG 120

Query: 121 GAHK-------------------------------------------------------- 180
             HK                                                        
Sbjct: 121 STHKYMRGALLSIISPTLIRDQLLPKIDEFMRTHLMDWDNKVINIQEKTKEMAFLSSLKQ 180

Query: 181 -----------------------------------------ARKNIIKLLQQLINERRAS 240
                                                    ARK+II +L QL+ ER+AS
Sbjct: 181 IAGMESSSIAQPFMTEFFKLVLGTLSLPINLPRTNYRGGLQARKSIISILSQLLEERKAS 240

Query: 241 ETQKDDMLGFFM-SEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +    DMLG  M  +EN+YKL+DEE+ID +IT++YSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 QDVHVDMLGCLMKKDENRYKLSDEEIIDLVITIMYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 323
           ++R+EHFAIRE+K+ EDPID +DLK+M+FTRAVIFETSRLATIVNGVLRKTT DMELNG+
Sbjct: 301 EIRKEHFAIRERKKPEDPIDCNDLKSMRFTRAVIFETSRLATIVNGVLRKTTHDMELNGY 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot
Match: Q43147 (Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 448.7 bits (1153), Expect = 5.4e-125
Identity = 240/464 (51.72%), Postives = 285/464 (61.42%), Query Frame = 0


Query: 1   MALALAILALFLLLC-FCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLK-------- 60
           MA  L  L+ F  LC FC+ALLRWN+V+Y +K LPPGTMGWP FGETTEFLK        
Sbjct: 1   MAFFLIFLSSFFGLCIFCTALLRWNQVKYNQKNLPPGTMGWPLFGETTEFLKLGPSFMKN 60

Query: 61  -----------------------------------QGLVPGYPQSMLDILGKCNIAAVHG 120
                                              +GLVPGYPQSM+DILGKCNIAAV+G
Sbjct: 61  QRARYGSFFKSHILGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMIDILGKCNIAAVNG 120

Query: 121 GAHK-------------------------------------------------------- 180
            AHK                                                        
Sbjct: 121 SAHKYMRGALLSLISPTMIRDQLLPKIDEFMRSHLTNWDNKVIDIQEKTNKMAFLSSLKQ 180

Query: 181 -----------------------------------------ARKNIIKLLQQLINERRAS 240
                                                    ARK I+ LL+ LI ERRAS
Sbjct: 181 IAGIESTSLAQEFMSEFFNLVLGTLSLPINLPNTNYHRGFQARKIIVNLLRTLIEERRAS 240

Query: 241 ETQKDDMLGFFMSEE-NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQ 300
           +  + DMLG+ M+EE  ++KL D+E+ID IIT+LYSGYETVSTTSMMAVK+LHDHPKVL+
Sbjct: 241 KEIQHDMLGYLMNEEATRFKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLE 300

Query: 301 QLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGF 323
           +LR+EH AIREKK+ EDPID++D ++M+FTRAVI ETSRLATIVNGVLRKTT+DME+NG+
Sbjct: 301 ELRKEHMAIREKKKPEDPIDYNDYRSMRFTRAVILETSRLATIVNGVLRKTTQDMEINGY 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot
Match: Q9FMA5 (Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 2.6e-119
Identity = 226/463 (48.81%), Postives = 275/463 (59.40%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ--------- 61
           A+ + +  L +++  CSALLRWN++RY + GLPPGTMGWP FGETTEFLKQ         
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ----------------------------------GLVPGYPQSMLDILGKCNIAAVHGGA 121
                                             GLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HK---------------------------------------------------------- 181
           H+                                                          
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 ----------------------------------------ARKNIIKLLQQLINERRASE 241
                                                   AR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TQKDDMLGFFMSEE-NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLG+ M +E N+Y L DEE+ DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 LREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 323
           LR EH A RE+KR ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+L
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot
Match: Q940V4 (Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 4.9e-118
Identity = 225/465 (48.39%), Postives = 274/465 (58.92%), Query Frame = 0


Query: 1   MALALAILALF-LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ------- 60
           M + + IL L  +++C C+ALLRWN++RY +KGLPPGTMGWP FGETTEFLKQ       
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  ------------------------------------GLVPGYPQSMLDILGKCNIAAVHG 120
                                               GLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHK-------------------------------------------------------- 180
            +H+                                                        
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 ------------------------------------------ARKNIIKLLQQLINERRA 240
                                                     AR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SETQKDDMLGFFM-SEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M  E+N+Y L D+E+ DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 323
           ++LR EH AIRE+KR ++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot
Match: Q50LE0 (Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 6.4e-118
Identity = 228/467 (48.82%), Postives = 274/467 (58.67%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSA-LLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ------- 60
           MA+ L I  +F   C  S  L RW ++ Y +K LPPGTMGWP FGET EFL Q       
Sbjct: 1   MAIFLIIFVVFFGFCILSTPLFRWIDIVYNKKNLPPGTMGWPIFGETREFLNQGPNFMKN 60

Query: 61  ------------------------------------GLVPGYPQSMLDILGKCNIAAVHG 120
                                               GL+PGYPQSMLDILGKCNIAAVHG
Sbjct: 61  QRARYGNFFKSHILGCPTVVSMDAGLNVYILNNEAKGLIPGYPQSMLDILGKCNIAAVHG 120

Query: 121 GAHK-------------------------------------------------------- 180
             HK                                                        
Sbjct: 121 ATHKYIRGALLSLINPTMIKDHILPKIDKFMRSHLSGWDNCNVIDIQQMTKEMAFFSSLD 180

Query: 181 -------------------------------------------ARKNIIKLLQQLINERR 240
                                                       RK I+KLL+++I +RR
Sbjct: 181 QIGGFATSSSIAQEFRAGFLNIALGTISLPINFPTTNYYRGLQGRKTIVKLLRKIIEDRR 240

Query: 241 ASETQKDDMLGFFMSEE--NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPK 300
            S+  + DMLG  M+EE  N+Y L+DEE+IDQIIT++YSG+ETVSTTSMMAVK+LHDHPK
Sbjct: 241 GSKKIQQDMLGLMMNEEAKNRYTLSDEELIDQIITIMYSGFETVSTTSMMAVKYLHDHPK 300

Query: 301 VLQQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMEL 323
            L+++R+EHFAIREKK  EDPID++D KAM+FTRAVI+ET RLATIVNGVLRKTT+DMEL
Sbjct: 301 ALEEIRKEHFAIREKKSLEDPIDYNDFKAMRFTRAVIYETLRLATIVNGVLRKTTQDMEL 360

[Close]

BLAST of Cp4.1LG01g22450 vs. NCBI nr
Match: XP_023524460.1 (cytochrome P450 85A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596 bits (1536), Expect = 9.46e-212
Identity = 322/462 (69.70%), Postives = 322/462 (69.70%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG       
Sbjct: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARKNIIKLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLQQLINERRASE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. NCBI nr
Match: KAG6602538.1 (Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 590 bits (1521), Expect = 1.81e-209
Identity = 318/462 (68.83%), Postives = 320/462 (69.26%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILAL LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARKNI+KLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. NCBI nr
Match: XP_022953184.1 (cytochrome P450 85A-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 590 bits (1521), Expect = 1.81e-209
Identity = 318/462 (68.83%), Postives = 320/462 (69.26%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILAL LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYPFLFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARKNI+KLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. NCBI nr
Match: KAG7033215.1 (Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587 bits (1513), Expect = 2.99e-208
Identity = 317/462 (68.61%), Postives = 319/462 (69.05%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILAL LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYILMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARKNIIKLL+QLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIKLLEQLINERRASE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEH AIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. NCBI nr
Match: XP_022990985.1 (cytochrome P450 85A-like [Cucurbita maxima])

HSP 1 Score: 584 bits (1506), Expect = 3.47e-207
Identity = 316/462 (68.40%), Postives = 319/462 (69.05%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILALFLLLCFCSALLRWNEVRYRRKGLP GTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALFLLLCFCSALLRWNEVRYRRKGLPHGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARK+IIKLLQQLINERRA+E
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKSIIKLLQQLINERRATE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEH AIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL
Match: A0A6J1GNX1 (cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111455792 PE=3 SV=1)

HSP 1 Score: 590 bits (1521), Expect = 8.78e-210
Identity = 318/462 (68.83%), Postives = 320/462 (69.26%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILAL LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALVLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYPFLFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARKNI+KLLQQLINERRASE
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIVKLLQQLINERRASE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL
Match: A0A6J1JRL1 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1)

HSP 1 Score: 584 bits (1506), Expect = 1.68e-207
Identity = 316/462 (68.40%), Postives = 319/462 (69.05%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+AILALFLLLCFCSALLRWNEVRYRRKGLP GTMGWPFFGETTEFLKQG       
Sbjct: 1   MALAIAILALFLLLCFCSALLRWNEVRYRRKGLPHGTMGWPFFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHGG
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGG 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKVMRAALLSVVSPTMIRDRLLPKVDDFMRSHLRNWDQQIIDIQEKTKEMALRSSMKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARK+IIKLLQQLINERRA+E
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDLPGTNYRRGIQARKSIIKLLQQLINERRATE 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEH AIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL
Match: A0A5D3BLH8 (Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002320 PE=3 SV=1)

HSP 1 Score: 563 bits (1450), Expect = 1.07e-199
Identity = 298/416 (71.63%), Postives = 307/416 (73.80%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+ ILALFLLL FCSALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQG       
Sbjct: 1   MALAMVILALFLLLLFCSALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVSMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHK--------------------------------------------------ARKNIIK 180
           AHK                                                  ARKNIIK
Sbjct: 121 AHKLMRGALLAIIAGFESGPLSESFTPEFFKLVLGTLSLPIDLPGTNYRRGIQARKNIIK 180

Query: 181 LLQQLINERRASETQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAV 240
           LL+QLI ERR S+ QK+DMLG+FMSEENKYKLNDEE+IDQIITVLYSGYETVSTTSMMAV
Sbjct: 181 LLEQLIKERRDSKIQKNDMLGYFMSEENKYKLNDEEIIDQIITVLYSGYETVSTTSMMAV 240

Query: 241 KFLHDHPKVLQQLREEHFAIREKKR-AEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVL 300
           KFLHDHPKVLQQLREEH AIREKK+  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVL
Sbjct: 241 KFLHDHPKVLQQLREEHLAIREKKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVL 300

Query: 301 RKTTKDMELNGFLIPKGWRIYVYTREINYDAFLYSEPLTFNPWRWLDKNLESHNYFFIFG 322
           RKTTKDMELNGFLIPKGWRIYVYTREINYD FLYSEP TFNPWRWLD NLES+NYFFIFG
Sbjct: 301 RKTTKDMELNGFLIPKGWRIYVYTREINYDPFLYSEPFTFNPWRWLDNNLESNNYFFIFG 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL
Match: A0A6J1FN01 (cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 3.92e-199
Identity = 303/462 (65.58%), Postives = 313/462 (67.75%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+ ILALFLLLCFC+ALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQG       
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARK+IIKLLQ+LI+ERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKSIIKLLQKLIDERRASK 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEH AIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL
Match: A0A6J1JUV6 (cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1)

HSP 1 Score: 561 bits (1447), Expect = 1.59e-198
Identity = 303/462 (65.58%), Postives = 312/462 (67.53%), Query Frame = 0


Query: 1   MALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQG------- 60
           MALA+ ILALFLLLCFC+ALLRWNEVRY +KGLPPGTMGWP FGETTEFLKQG       
Sbjct: 1   MALAMVILALFLLLCFCAALLRWNEVRYSKKGLPPGTMGWPIFGETTEFLKQGPNFMKSQ 60

Query: 61  ------------------------------------LVPGYPQSMLDILGKCNIAAVHGG 120
                                               LVPGYPQSMLDILGKCNIAAVHG 
Sbjct: 61  RARYGSIFKSHILGCPTIVCMDPEVNRYVLMNESKGLVPGYPQSMLDILGKCNIAAVHGS 120

Query: 121 AHK--------------------------------------------------------- 180
           AHK                                                         
Sbjct: 121 AHKLMRAALLSVVSPTMIKDRLLPKVDEFMRSHLRNWDRQIIDIQDKTKEMALCSSLKQI 180

Query: 181 ----------------------------------------ARKNIIKLLQQLINERRASE 240
                                                   ARK IIKLLQ+LI+ERRAS+
Sbjct: 181 AGIESGPLSESFIPEFFKLVLGTLSLPIDFPGTNYRQGIQARKIIIKLLQKLIDERRASK 240

Query: 241 TQKDDMLGFFMSEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300
           T+KDDMLGFFMSEENKYKL DEE+IDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL
Sbjct: 241 TEKDDMLGFFMSEENKYKLKDEEIIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQL 300

Query: 301 REEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 322
           REEH AIREKK  EDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI
Sbjct: 301 REEHLAIREKKNPEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFLI 360

[Close]

BLAST of Cp4.1LG01g22450 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 429.9 bits (1104), Expect = 1.8e-120
Identity = 226/463 (48.81%), Postives = 275/463 (59.40%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ--------- 61
           A+ + +  L +++  CSALLRWN++RY + GLPPGTMGWP FGETTEFLKQ         
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ----------------------------------GLVPGYPQSMLDILGKCNIAAVHGGA 121
                                             GLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HK---------------------------------------------------------- 181
           H+                                                          
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 ----------------------------------------ARKNIIKLLQQLINERRASE 241
                                                   AR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TQKDDMLGFFMSEE-NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 301
               DMLG+ M +E N+Y L DEE+ DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

Query: 302 LREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNGFL 323
           LR EH A RE+KR ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT+D+E+NG+L
Sbjct: 303 LRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEINGYL 362

[Close]

BLAST of Cp4.1LG01g22450 vs. TAIR 10
Match: AT3G30180.1 (brassinosteroid-6-oxidase 2 )

HSP 1 Score: 425.6 bits (1093), Expect = 3.5e-119
Identity = 225/465 (48.39%), Postives = 274/465 (58.92%), Query Frame = 0


Query: 1   MALALAILALF-LLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ------- 60
           M + + IL L  +++C C+ALLRWN++RY +KGLPPGTMGWP FGETTEFLKQ       
Sbjct: 1   MGIMMMILGLLVIIVCLCTALLRWNQMRYSKKGLPPGTMGWPIFGETTEFLKQGPDFMKN 60

Query: 61  ------------------------------------GLVPGYPQSMLDILGKCNIAAVHG 120
                                               GLV GYPQSMLDILG CNIAAVHG
Sbjct: 61  QRLRYGSFFKSHILGCPTIVSMDAELNRYILMNESKGLVAGYPQSMLDILGTCNIAAVHG 120

Query: 121 GAHK-------------------------------------------------------- 180
            +H+                                                        
Sbjct: 121 PSHRLMRGSLLSLISPTMMKDHLLPKIDDFMRNYLCGWDDLETVDIQEKTKHMAFLSSLL 180

Query: 181 ------------------------------------------ARKNIIKLLQQLINERRA 240
                                                     AR NI +LL +L+ ER+ 
Sbjct: 181 QIAETLKKPEVEEYRTEFFKLVVGTLSVPIDIPGTNYRSGVQARNNIDRLLTELMQERKE 240

Query: 241 SETQKDDMLGFFM-SEENKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVL 300
           S     DMLG+ M  E+N+Y L D+E+ DQ++T+LYSGYETVSTTSMMA+K+LHDHPK L
Sbjct: 241 SGETFTDMLGYLMKKEDNRYLLTDKEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 300

Query: 301 QQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTTKDMELNG 323
           ++LR EH AIRE+KR ++P+  DD+K+MKFTRAVIFETSRLATIVNGVLRKTT D+ELNG
Sbjct: 301 EELRREHLAIRERKRPDEPLTLDDIKSMKFTRAVIFETSRLATIVNGVLRKTTHDLELNG 360

[Close]

BLAST of Cp4.1LG01g22450 vs. TAIR 10
Match: AT5G38970.2 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-104
Identity = 185/292 (63.36%), Postives = 227/292 (77.74%), Query Frame = 0


Query: 36  GTMGWPFFGE-TTEFLKQGLVPGYPQSMLDILG---KCNIAAVHGGAHKARKNIIKLLQQ 95
           G +  PF  E  T F K  LV G     +D+ G   +C I        +AR NI +LL++
Sbjct: 103 GNLRKPFVEEFKTAFFK--LVVGTLSVPIDLPGTNYRCGI--------QARNNIDRLLRE 162

Query: 96  LINERRASETQKDDMLGFFMSEE-NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFL 155
           L+ ERR S     DMLG+ M +E N+Y L DEE+ DQ++T+LYSGYETVSTTSMMA+K+L
Sbjct: 163 LMQERRDSGETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYL 222

Query: 156 HDHPKVLQQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIFETSRLATIVNGVLRKTT 215
           HDHPK LQ+LR EH A RE+KR ++P+  +D+K+MKFTRAVI+ETSRLATIVNGVLRKTT
Sbjct: 223 HDHPKALQELRAEHLAFRERKRQDEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTT 282

Query: 216 KDMELNGFLIPKGWRIYVYTREINYDAFLYSEPLTFNPWRWLDKNLESHNYFFIFGGGTR 275
           +D+E+NG+LIPKGWRIYVYTREINYDA LY +PL FNPWRW+ K+LES N  F+FGGGTR
Sbjct: 283 RDLEINGYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTR 342

Query: 276 HCPGKELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEAPSGFHIRVSSY 323
            CPGKELGI EIS+FLHYFVT+YRWEE+GGD+L+ FPRV AP GFH+R+S Y
Sbjct: 343 LCPGKELGIVEISSFLHYFVTRYRWEEIGGDELMVFPRVFAPKGFHLRISPY 384

HSP 2 Score: 56.2 bits (134), Expect = 5.5e-08
Identity = 28/70 (40.00%), Postives = 42/70 (60.00%), Query Frame = 0


Query: 52  QGLVPGYPQSMLDILGKCNIAAVHGGAHKARKNIIKLLQQLINERRASETQKDDMLGFFM 111
           +GLVPGYPQSMLDILG CN+AAVHG +H+  +  +  L      R     + D  +  ++
Sbjct: 15  KGLVPGYPQSMLDILGTCNMAAVHGSSHRLMRGSLLSLISSTMMRDHILPKVDHFMRSYL 74

Query: 112 SEENKYKLND 122
            + N+ ++ D
Sbjct: 75  DQWNELEVID 84

[Close]

BLAST of Cp4.1LG01g22450 vs. TAIR 10
Match: AT5G38970.3 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 312.4 bits (799), Expect = 4.3e-85
Identity = 175/393 (44.53%), Postives = 215/393 (54.71%), Query Frame = 0


Query: 2   ALALAILALFLLLCFCSALLRWNEVRYRRKGLPPGTMGWPFFGETTEFLKQ--------- 61
           A+ + +  L +++  CSALLRWN++RY + GLPPGTMGWP FGETTEFLKQ         
Sbjct: 3   AMMVMMGLLLIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLKQGPNFMRNQR 62

Query: 62  ----------------------------------GLVPGYPQSMLDILGKCNIAAVHGGA 121
                                             GLVPGYPQSMLDILG CN+AAVHG +
Sbjct: 63  LRYGSFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSS 122

Query: 122 HK---------------------------------------------------------- 181
           H+                                                          
Sbjct: 123 HRLMRGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKHMAFLSSLTQI 182

Query: 182 ----------------------------------------ARKNIIKLLQQLINERRASE 241
                                                   AR NI +LL++L+ ERR S 
Sbjct: 183 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 242

Query: 242 TQKDDMLGFFMSEE-NKYKLNDEEVIDQIITVLYSGYETVSTTSMMAVKFLHDHPKVLQQ 253
               DMLG+ M +E N+Y L DEE+ DQ++T+LYSGYETVSTTSMMA+K+LHDHPK LQ+
Sbjct: 243 ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKALQE 302

[Close]

BLAST of Cp4.1LG01g22450 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 184.9 bits (468), Expect = 1.0e-46
Identity = 95/248 (38.31%), Postives = 143/248 (57.66%), Query Frame = 0


Query: 78  AHKARKNIIKLLQQLINERRASETQ----KDDMLGFFMSEENKYKLNDEEVIDQIITVLY 137
           A +AR+ + + L  ++ +RR  E +    K DML   ++ ++ +  +DEE++D ++ +L 
Sbjct: 220 AIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAADDGF--SDEEIVDFLVALLV 279

Query: 138 SGYETVSTTSMMAVKFLHDHPKVLQQLREEHFAIREKKRAEDPIDWDDLKAMKFTRAVIF 197
           +GYET ST   +AVKFL + P  L QL+EEH  IR  K     ++W D K+M FT+ V+ 
Sbjct: 280 AGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEWSDYKSMPFTQCVVN 339

Query: 198 ETSRLATIVNGVLRKTTKDMELNGFLIPKGWRIYVYTREINYDAFLYSEPLTFNPWRWLD 257
           ET R+A I+ GV R+   D+E+ G+ IPKGW+++   R ++ D   + +  TFNPWRW  
Sbjct: 340 ETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFKDARTFNPWRWQS 399

Query: 258 KNLES--HNYFFIFGGGTRHCPGKELGIAEISTFLHYFVTKYRWEEVGGDKLLKFPRVEA 317
            ++ +   N F  FGGG R CPG EL    +S FLH  VT + W     DKL+ FP    
Sbjct: 400 NSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAEQDKLVFFPTTRT 459

Query: 318 PSGFHIRV 320
              + I V
Sbjct: 460 QKRYPIFV 465

HSP 2 Score: 39.3 bits (90), Expect = 7.0e-03
Identity = 22/48 (45.83%), Postives = 28/48 (58.33%), Query Frame = 0


Query: 5  LAILALFLLLCFCSA--LLRWNEVRYRRKGLPPGTMGWPFFGETTEFL 51
          +A  A  LLL   +A  LL     RYRR GLPPG++G P  GET + +
Sbjct: 1  MAFTAFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLI 48

The following BLAST results are available for this feature:

BLAST of Cp4.1LG01g22450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69F95	6.0e-132	55.17	Cytochrome P450 85A OS=Phaseolus vulgaris OX=3885 GN=BA13 PE=3 SV=2	[more]
Q43147	5.4e-125	51.72	Cytochrome P450 85A1 OS=Solanum lycopersicum OX=4081 GN=CYP85A1 PE=2 SV=1	[more]
Q9FMA5	2.6e-119	48.81	Cytochrome P450 85A1 OS=Arabidopsis thaliana OX=3702 GN=CYP85A1 PE=2 SV=1	[more]
Q940V4	4.9e-118	48.39	Cytochrome P450 85A2 OS=Arabidopsis thaliana OX=3702 GN=CYP85A2 PE=2 SV=1	[more]
Q50LE0	6.4e-118	48.82	Cytochrome P450 85A3 OS=Solanum lycopersicum OX=4081 GN=CYP85A3 PE=2 SV=1	[more]

BLAST of Cp4.1LG01g22450 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524460.1	9.46e-212	69.70	cytochrome P450 85A-like [Cucurbita pepo subsp. pepo]	[more]
KAG6602538.1	1.81e-209	68.83	Cytochrome P450 85A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022953184.1	1.81e-209	68.83	cytochrome P450 85A-like isoform X2 [Cucurbita moschata]	[more]
KAG7033215.1	2.99e-208	68.61	Cytochrome P450 85A [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022990985.1	3.47e-207	68.40	cytochrome P450 85A-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG01g22450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GNX1	8.78e-210	68.83	cytochrome P450 85A-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11145579...	[more]
A0A6J1JRL1	1.68e-207	68.40	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111487713 PE=3 SV=1	[more]
A0A5D3BLH8	1.07e-199	71.63	Cytochrome P450 85A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1FN01	3.92e-199	65.58	cytochrome P450 85A-like OS=Cucurbita moschata OX=3662 GN=LOC111445417 PE=3 SV=1	[more]
A0A6J1JUV6	1.59e-198	65.58	cytochrome P450 85A-like OS=Cucurbita maxima OX=3661 GN=LOC111489110 PE=3 SV=1	[more]

BLAST of Cp4.1LG01g22450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38970.1	1.8e-120	48.81	brassinosteroid-6-oxidase 1	[more]
AT3G30180.1	3.5e-119	48.39	brassinosteroid-6-oxidase 2	[more]
AT5G38970.2	3.2e-104	63.36	brassinosteroid-6-oxidase 1	[more]
AT5G38970.3	4.3e-85	44.53	brassinosteroid-6-oxidase 1	[more]
AT5G05690.1	1.0e-46	38.31	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 272..295 score: 23.57 coord: 229..253 score: 27.49 coord: 123..140 score: 21.17 coord: 189..207 score: 30.9 coord: 143..169 score: 20.46 coord: 262..272 score: 45.3
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 134..151 score: 29.56 coord: 263..272 score: 46.66 coord: 272..283 score: 31.72 coord: 190..201 score: 41.33
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 77..302 e-value: 1.6E-51 score: 175.5
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 31..306 e-value: 6.0E-65 score: 222.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 74..303
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 81..320
None	No IPR available	PANTHER	PTHR24286:SF273	CYTOCHROME P450 FAMILY PROTEIN	coord: 7..53
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 7..53
None	No IPR available	PANTHER	PTHR24286:SF273	CYTOCHROME P450 FAMILY PROTEIN	coord: 52..80
None	No IPR available	PANTHER	PTHR24286:SF273	CYTOCHROME P450 FAMILY PROTEIN	coord: 81..320
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 52..80
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 265..274

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG01g22450.1	Cp4.1LG01g22450.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen