Cp4.1LG01g21330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g21330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUPF0496 protein 4-like
LocationCp4.1LG01: 17924165 .. 17925316 (-)
RNA-Seq ExpressionCp4.1LG01g21330
SyntenyCp4.1LG01g21330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAGCTACTGATTATCAGGGTTCATCGGCTGCTTGGGGTGTTCGGCGAGATCAGCTTTACGCTATGACTGATTCTCCGACGTCTCATGAACAGGATCTTGACGGGTTTCAGAGGCAAGTTGCTGATCGGTTCTTAGAGCTTGCGTCGGTTGGTGCTGATGATTTGCTTTCCTTGTCTTGGGTTCAGAAGCTTTTGGATTCGTTTTTGTGCTGTCAGGAGGAGTTTAAGCTCATTCTCGACAGCCAAAAATCTCTAATCTCTAAAGCTCCATTGGATCGATTGGTTGCTGATTATTCGGAGCGTAGCGTTAAGGCGCTTGATGTGTGTAATGCGATTCGTGATGGGGTTGAGCAGCTACGGCAATGGCAGAAGCTCTTGGAGATTGTTGTCAGTGCGTTGGACAATTGTAATCACCAGAAAACTCTTGGGGAGGGCCAATTTCGTCGCGCTAAGAAGGCCCTCACCGATTTGGCTATCTGTATGCTTGATGAGAAAGATTCGCATACCTCTTTCCTTGCCCACCGCAACCGTTCTTTCGGACGGAACAATGTCACGAAAGATCCACGGTCTTTGGGCCACTTTCGATCTCTGTCATGGAGCGTTTCGCGATCGTGGTCGGCCGCACGGCAGCTACAATCGATTGGTAACAATTTGGCGGCCCCTAAAGCGACTGAGCTTTTGGCTACTAATGGGCTTGCAGTTCCTATTTTCACCATGAACATGGTGTTATTGTTTGTAATGTGGGCGCTGGTAGCGGCTATTCCTTGCCAGGATCGGGGTTTGCAGGTTCATTTCTCGTTGCCTCGGAGTTTCCCATGGGCGGCTTCAATCCTTCAACTCCATGATCGAATTGTGGAGGAGTCCAGGAAGAGAGATCGAAGGAATTCCTGTGGGCTGTTGAAGGAGATTCATCAGATTGAAAAGTGCACGCGTCTCATGAATGATTTGGCCGATGCAGCTCAGTTCCCGTTGGCTGAGGAGAAAGAAGCAGAGCTGAGACAGAGAGTGCAAGAGCTAACCGGGGTTTGTAATACTCTGAGGACTGGATTGGACTCAATGGAGCGGCAGGTGAGGGAAGTTTTTCACAGGATTGTTCGCAGCAGGACTGAAGGGCTAGACTCTTTGGGACGAGCTAACCACTCTGAATAG

mRNA sequence

ATGCCAGCTACTGATTATCAGGGTTCATCGGCTGCTTGGGGTGTTCGGCGAGATCAGCTTTACGCTATGACTGATTCTCCGACGTCTCATGAACAGGATCTTGACGGGTTTCAGAGGCAAGTTGCTGATCGGTTCTTAGAGCTTGCGTCGGTTGGTGCTGATGATTTGCTTTCCTTGTCTTGGGTTCAGAAGCTTTTGGATTCGTTTTTGTGCTGTCAGGAGGAGTTTAAGCTCATTCTCGACAGCCAAAAATCTCTAATCTCTAAAGCTCCATTGGATCGATTGGTTGCTGATTATTCGGAGCGTAGCGTTAAGGCGCTTGATGTGTGTAATGCGATTCGTGATGGGGTTGAGCAGCTACGGCAATGGCAGAAGCTCTTGGAGATTGTTGTCAGTGCGTTGGACAATTGTAATCACCAGAAAACTCTTGGGGAGGGCCAATTTCGTCGCGCTAAGAAGGCCCTCACCGATTTGGCTATCTGTATGCTTGATGAGAAAGATTCGCATACCTCTTTCCTTGCCCACCGCAACCGTTCTTTCGGACGGAACAATGTCACGAAAGATCCACGGTCTTTGGGCCACTTTCGATCTCTGTCATGGAGCGTTTCGCGATCGTGGTCGGCCGCACGGCAGCTACAATCGATTGGTAACAATTTGGCGGCCCCTAAAGCGACTGAGCTTTTGGCTACTAATGGGCTTGCAGTTCCTATTTTCACCATGAACATGGTGTTATTGTTTGTAATGTGGGCGCTGGTAGCGGCTATTCCTTGCCAGGATCGGGGTTTGCAGGTTCATTTCTCGTTGCCTCGGAGTTTCCCATGGGCGGCTTCAATCCTTCAACTCCATGATCGAATTGTGGAGGAGTCCAGGAAGAGAGATCGAAGGAATTCCTGTGGGCTGTTGAAGGAGATTCATCAGATTGAAAAGTGCACGCGTCTCATGAATGATTTGGCCGATGCAGCTCAGTTCCCGTTGGCTGAGGAGAAAGAAGCAGAGCTGAGACAGAGAGTGCAAGAGCTAACCGGGGTTTGTAATACTCTGAGGACTGGATTGGACTCAATGGAGCGGCAGGTGAGGGAAGTTTTTCACAGGATTGTTCGCAGCAGGACTGAAGGGCTAGACTCTTTGGGACGAGCTAACCACTCTGAATAG

Coding sequence (CDS)

ATGCCAGCTACTGATTATCAGGGTTCATCGGCTGCTTGGGGTGTTCGGCGAGATCAGCTTTACGCTATGACTGATTCTCCGACGTCTCATGAACAGGATCTTGACGGGTTTCAGAGGCAAGTTGCTGATCGGTTCTTAGAGCTTGCGTCGGTTGGTGCTGATGATTTGCTTTCCTTGTCTTGGGTTCAGAAGCTTTTGGATTCGTTTTTGTGCTGTCAGGAGGAGTTTAAGCTCATTCTCGACAGCCAAAAATCTCTAATCTCTAAAGCTCCATTGGATCGATTGGTTGCTGATTATTCGGAGCGTAGCGTTAAGGCGCTTGATGTGTGTAATGCGATTCGTGATGGGGTTGAGCAGCTACGGCAATGGCAGAAGCTCTTGGAGATTGTTGTCAGTGCGTTGGACAATTGTAATCACCAGAAAACTCTTGGGGAGGGCCAATTTCGTCGCGCTAAGAAGGCCCTCACCGATTTGGCTATCTGTATGCTTGATGAGAAAGATTCGCATACCTCTTTCCTTGCCCACCGCAACCGTTCTTTCGGACGGAACAATGTCACGAAAGATCCACGGTCTTTGGGCCACTTTCGATCTCTGTCATGGAGCGTTTCGCGATCGTGGTCGGCCGCACGGCAGCTACAATCGATTGGTAACAATTTGGCGGCCCCTAAAGCGACTGAGCTTTTGGCTACTAATGGGCTTGCAGTTCCTATTTTCACCATGAACATGGTGTTATTGTTTGTAATGTGGGCGCTGGTAGCGGCTATTCCTTGCCAGGATCGGGGTTTGCAGGTTCATTTCTCGTTGCCTCGGAGTTTCCCATGGGCGGCTTCAATCCTTCAACTCCATGATCGAATTGTGGAGGAGTCCAGGAAGAGAGATCGAAGGAATTCCTGTGGGCTGTTGAAGGAGATTCATCAGATTGAAAAGTGCACGCGTCTCATGAATGATTTGGCCGATGCAGCTCAGTTCCCGTTGGCTGAGGAGAAAGAAGCAGAGCTGAGACAGAGAGTGCAAGAGCTAACCGGGGTTTGTAATACTCTGAGGACTGGATTGGACTCAATGGAGCGGCAGGTGAGGGAAGTTTTTCACAGGATTGTTCGCAGCAGGACTGAAGGGCTAGACTCTTTGGGACGAGCTAACCACTCTGAATAG

Protein sequence

MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVREVFHRIVRSRTEGLDSLGRANHSE
Homology
BLAST of Cp4.1LG01g21330 vs. ExPASy Swiss-Prot
Match: Q9CAK4 (Protein ROH1 OS=Arabidopsis thaliana OX=3702 GN=ROH1 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.2e-68
Identity = 152/414 (36.71%), Postives = 250/414 (60.39%), Query Frame = 0

Query: 2   PATDYQGS-SAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLEL------------ 61
           PA D QGS      +RR+Q   + +     ++DL+ FQ+ +ADRF EL            
Sbjct: 3   PAQDNQGSFLGRISIRRNQFVDVNNE--QEQEDLELFQKHIADRFTELLSPPQPPPSDEI 62

Query: 62  ---ASVGA-DDLLSLSWVQKLLDSFLCCQEEFK--LILDSQKSLISKAPLDRLVADYSER 121
              ASV A + ++S++W++KL+D FLCC+ EFK  L++    + ISK P DRLV +  +R
Sbjct: 63  NTVASVAATEQIMSVTWLRKLMDVFLCCEAEFKAILLMGRDPTQISKPPFDRLVPEMLDR 122

Query: 122 SVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICM 181
           S+KALD+C A+ +G++ +R +Q+L EI V+AL+    Q+ LG+G  RRAK+AL +L + +
Sbjct: 123 SIKALDICTAVVNGIDSVRHYQRLAEIAVTALE----QRPLGDGNVRRAKRALANLVVAL 182

Query: 182 -LDEKDSHT---------SFLAHRNRSFGRNN-----VTKDPRSLGHFRSLSWSVSRSWS 241
            L++K++ +         +    R+ SFGR +      +K   ++G  +S SW+V R+WS
Sbjct: 183 SLEDKENVSGGGGGGGGGNKTTERSWSFGRRSGGSSAASKGGATIGQLKSSSWAVGRNWS 242

Query: 242 AARQLQSIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDR-GLQVHF 301
           AA+Q+ ++  NL  P+  E     GL  P+F M+ V++FVMW L AA+PCQ+R GL  H 
Sbjct: 243 AAKQIHAMTANLTPPRGNE---AAGLPQPMFIMSTVMVFVMWVLTAAVPCQERSGLANHL 302

Query: 302 SL-PRSFPWAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPL 361
            + P+   WA S++ +H++I +E +K++++ S GL++E+ ++EK    + + AD   +P 
Sbjct: 303 PVPPKHLNWAQSLIGIHEKIGDEWKKKEKKGSAGLMEEMTRMEKLGHSLMEFADGFHYPA 362

Query: 362 AEEKEAELRQRVQELTGVCNTLRTGLDSMERQVREVFHRIVRSRTEGLDSLGRA 380
            ++       +V E+  +C  +   L  +++Q+REVFHRIVRSR E L+ L +A
Sbjct: 363 EKDAAESAAVQVAEMAEICRRMEEELVPLQQQIREVFHRIVRSRAEILEVLEQA 407

BLAST of Cp4.1LG01g21330 vs. ExPASy Swiss-Prot
Match: Q337C0 (UPF0496 protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0513300 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 6.4e-08
Identity = 67/304 (22.04%), Postives = 118/304 (38.82%), Query Frame = 0

Query: 34  LDGFQRQVADRFLELASVGADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLD 93
           L  ++  +A    +L    A D+L+LSW++  +D           ++   +  +S    D
Sbjct: 36  LASYEDALALSLRKLKPEAASDVLTLSWMRLAVDCLSELHTNIANLITDLELPVSDWD-D 95

Query: 94  RLVADYSERSVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKK 153
           + V  Y   SVK LD+C A+   + +L Q Q LL+  +  L   +      + Q +RA+ 
Sbjct: 96  KWVDIYLNSSVKLLDICIALSSELSRLDQGQLLLQYALHVLG--SESGVPSQEQLKRAEP 155

Query: 154 ALTDLAICMLDEKDSHTSFLAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQ 213
           +L +                                        +    +R  S +  LQ
Sbjct: 156 SLRE------------------------------------WMELVGVRCARLVSCSATLQ 215

Query: 214 SIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFP 273
            +  NL+  K         L   ++ +  V +FV    VA +    + L V   +P  F 
Sbjct: 216 ELAGNLSLMKVKNSAKGKVLMRALYGIESVTVFVCSIFVAVLSGSPKPL-VELHVPEKFG 275

Query: 274 WAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAEL 333
           W+ +   LH  + EE  ++    S   +KE+ ++E C R ++ LA  +Q    EE+ A L
Sbjct: 276 WSQAFNDLHTAVSEELTRQLSGGSVAAVKELEEVEACARRLHVLASTSQL---EEEAANL 296

Query: 334 RQRV 338
              V
Sbjct: 336 ANAV 296

BLAST of Cp4.1LG01g21330 vs. ExPASy Swiss-Prot
Match: A2Z9A6 (UPF0496 protein 4 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_033149 PE=3 SV=2)

HSP 1 Score: 59.7 bits (143), Expect = 8.4e-08
Identity = 68/304 (22.37%), Postives = 121/304 (39.80%), Query Frame = 0

Query: 34  LDGFQRQVADRFLELASVGADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLD 93
           L  ++  +A    +L    A D+L+LSW++  +D           ++   +  +S    D
Sbjct: 36  LASYEDALALSLRKLKPEAASDVLTLSWMRLAVDCLSELHTNIANLITDLELPVSDWD-D 95

Query: 94  RLVADYSERSVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKK 153
           + V  Y   SVK LD+C A+   + +L Q Q LL+  +  L   +      + Q +RA+ 
Sbjct: 96  KWVDIYLNSSVKLLDICIALSSELSRLDQGQLLLQYALHVLG--SESGVPSQEQLKRAEP 155

Query: 154 ALTDLAICMLDEKDSHTSFLAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQ 213
           +L                      R +      + PR +              S +  LQ
Sbjct: 156 SL----------------------REWMELVGVRCPRLV--------------SCSATLQ 215

Query: 214 SIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFP 273
            +  NL+  K    +    L   ++ +  V +FV    VA +    + L V   +P  F 
Sbjct: 216 ELAGNLSLMKVKNSVKGKVLMRALYGIESVTVFVCSIFVAVLSGSPKPL-VELHVPEKFG 275

Query: 274 WAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAEL 333
           W+ +   LH  + EE  ++    S   +KE+ ++E C + ++ LA  +Q    EE+ A L
Sbjct: 276 WSQAFNDLHTAVSEELTRQLAGGSVAAVKELEEVEACAKRLHVLASTSQL---EEEAANL 296

Query: 334 RQRV 338
              V
Sbjct: 336 ANAV 296

BLAST of Cp4.1LG01g21330 vs. ExPASy Swiss-Prot
Match: Q9LMM6 (Protein BPS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPS1 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 1.3e-05
Identity = 67/343 (19.53%), Postives = 136/343 (39.65%), Query Frame = 0

Query: 34  LDGFQRQVADRFLELASVGADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLD 93
           L+ F+  +A    +L      D+L++SW+++ ++S        K ++   +  +S    D
Sbjct: 36  LNNFETNLASSISKLVPKEKSDILTVSWMKQAMESLCETHNGIKTLITDLELPVSDWE-D 95

Query: 94  RLVADYSERSVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKK 153
           + V  Y + SVK LD+CNA    + +L Q   LL+  +  L+  N  + L + Q      
Sbjct: 96  KWVDVYLDISVKLLDLCNAFSSELTRLNQGHLLLQFALHNLE-ANSPQNLSKAQ------ 155

Query: 154 ALTDLAICMLDEKDSHTSFLAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQ 213
                    LD    H               V+K+PR + + R++             L 
Sbjct: 156 -------SSLDSWKQHI--------------VSKNPR-IENCRAI-------------LS 215

Query: 214 SIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFP 273
           S+   L  PK         L   ++ + +  L++     AA     + L ++ ++    P
Sbjct: 216 SLVQTLNLPKVKNSAKGKVLMRALYGVKVKTLYISGVFAAAFSGSSQNL-MYLTVSNELP 275

Query: 274 WAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAEL 333
           WA S +++ + +  E +     +   +LKE+  +    + +        +P  ++   + 
Sbjct: 276 WAQSFMEVQNTMNAEIKNIFLSDGLTVLKELEAVASGVKKL--------YPAIQQGSID- 325

Query: 334 RQRVQELTGVCNTLRTGLDSMERQVREVFHRIVRSRTEGLDSL 377
              +Q L      L  G+D + ++V   F  ++  R   L++L
Sbjct: 336 PISLQPLKDSVTELSNGIDLVSKEVDCFFKILLSGRDTLLENL 325

BLAST of Cp4.1LG01g21330 vs. NCBI nr
Match: XP_023528071.1 (UPF0496 protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755 bits (1949), Expect = 7.34e-275
Identity = 383/383 (100.00%), Postives = 383/383 (100.00%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE
Sbjct: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360

Query: 361 VFHRIVRSRTEGLDSLGRANHSE 383
           VFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 VFHRIVRSRTEGLDSLGRANHSE 383

BLAST of Cp4.1LG01g21330 vs. NCBI nr
Match: XP_022963767.1 (UPF0496 protein 4-like [Cucurbita moschata] >KAG7032872.1 hypothetical protein SDJN02_06922, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 748 bits (1931), Expect = 4.07e-272
Identity = 380/383 (99.22%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAMTDSPTSHE DLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEHDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKD RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDSRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAA ILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAAPILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE
Sbjct: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360

Query: 361 VFHRIVRSRTEGLDSLGRANHSE 383
           VFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 VFHRIVRSRTEGLDSLGRANHSE 383

BLAST of Cp4.1LG01g21330 vs. NCBI nr
Match: XP_022990383.1 (UPF0496 protein 4-like [Cucurbita maxima])

HSP 1 Score: 741 bits (1914), Expect = 1.59e-269
Identity = 377/383 (98.43%), Postives = 377/383 (98.43%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAM  SPT HEQDLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMNGSPTPHEQDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKD RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDSRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAA ILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAAPILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKC RLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE
Sbjct: 301 LKEIHQIEKCARLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360

Query: 361 VFHRIVRSRTEGLDSLGRANHSE 383
           VFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 VFHRIVRSRTEGLDSLGRANHSE 383

BLAST of Cp4.1LG01g21330 vs. NCBI nr
Match: KAG6602188.1 (Protein ROH1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 699 bits (1804), Expect = 4.93e-252
Identity = 354/362 (97.79%), Postives = 356/362 (98.34%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAMTDSPTSHE DLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEHDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKD RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDSRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAA ILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAAPILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQ   
Sbjct: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQDGR 360

Query: 361 VF 362
           ++
Sbjct: 361 IY 362

BLAST of Cp4.1LG01g21330 vs. NCBI nr
Match: XP_022939341.1 (uncharacterized protein LOC111445285 isoform X1 [Cucurbita moschata] >XP_022939342.1 uncharacterized protein LOC111445285 isoform X2 [Cucurbita moschata])

HSP 1 Score: 680 bits (1755), Expect = 3.63e-245
Identity = 345/391 (88.24%), Postives = 362/391 (92.58%), Query Frame = 0

Query: 1   MPATDYQGSSAAW--------GVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVG 60
           MPATDYQGSSAA          +RRDQLYAM  SPTS EQDLD FQRQVA+RF++LASVG
Sbjct: 1   MPATDYQGSSAACTNIGRSVQSIRRDQLYAMDGSPTSQEQDLDSFQRQVAERFMDLASVG 60

Query: 61  ADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNA 120
            D+LLSLSWVQKLLDSFLCCQEEFK +L + KS ISK PLDRLVADYSER+VKALDVCNA
Sbjct: 61  PDELLSLSWVQKLLDSFLCCQEEFKFVLINHKSQISKPPLDRLVADYSERTVKALDVCNA 120

Query: 121 IRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSF 180
           IRDG+EQLRQWQKLLEIV+SALDNCNHQKTLGEGQFRRAKKAL DLAICMLDEKDSH S 
Sbjct: 121 IRDGIEQLRQWQKLLEIVLSALDNCNHQKTLGEGQFRRAKKALVDLAICMLDEKDSHASA 180

Query: 181 LAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240
           +AHRNRSFGRNN +KDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG
Sbjct: 181 IAHRNRSFGRNNASKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240

Query: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKR 300
           LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSL R+FPWA  ILQLHDRIVEES+KR
Sbjct: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLSRNFPWAVPILQLHDRIVEESKKR 300

Query: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLD 360
           DRRNSCGLLKEIHQIEKCTRLMNDLAD AQFPLAEEKEAELR+RVQELT VCNTLRTGLD
Sbjct: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADTAQFPLAEEKEAELRERVQELTTVCNTLRTGLD 360

Query: 361 SMERQVREVFHRIVRSRTEGLDSLGRANHSE 383
           S+ERQVREVFHR+VRSRTEGLDSLGRANHSE
Sbjct: 361 SLERQVREVFHRVVRSRTEGLDSLGRANHSE 391

BLAST of Cp4.1LG01g21330 vs. ExPASy TrEMBL
Match: A0A6J1HG41 (UPF0496 protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111463959 PE=4 SV=1)

HSP 1 Score: 748 bits (1931), Expect = 1.97e-272
Identity = 380/383 (99.22%), Postives = 380/383 (99.22%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAMTDSPTSHE DLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEHDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKD RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDSRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAA ILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAAPILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE
Sbjct: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360

Query: 361 VFHRIVRSRTEGLDSLGRANHSE 383
           VFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 VFHRIVRSRTEGLDSLGRANHSE 383

BLAST of Cp4.1LG01g21330 vs. ExPASy TrEMBL
Match: A0A6J1JT46 (UPF0496 protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487256 PE=4 SV=1)

HSP 1 Score: 741 bits (1914), Expect = 7.68e-270
Identity = 377/383 (98.43%), Postives = 377/383 (98.43%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVGADDLLSLS 60
           MPATDYQGSSAAWGVRRDQLYAM  SPT HEQDLDGFQRQVADRFLELASVGADDLLSLS
Sbjct: 1   MPATDYQGSSAAWGVRRDQLYAMNGSPTPHEQDLDGFQRQVADRFLELASVGADDLLSLS 60

Query: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120
           WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL
Sbjct: 61  WVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGVEQL 120

Query: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180
           RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF
Sbjct: 121 RQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRNRSF 180

Query: 181 GRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240
           GRNNVTKD RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM
Sbjct: 181 GRNNVTKDSRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAVPIFTM 240

Query: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRDRRNSCGL 300
           NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAA ILQLHDRIVEESRKRDRRNSCGL
Sbjct: 241 NMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAAPILQLHDRIVEESRKRDRRNSCGL 300

Query: 301 LKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360
           LKEIHQIEKC RLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE
Sbjct: 301 LKEIHQIEKCARLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLDSMERQVRE 360

Query: 361 VFHRIVRSRTEGLDSLGRANHSE 383
           VFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 VFHRIVRSRTEGLDSLGRANHSE 383

BLAST of Cp4.1LG01g21330 vs. ExPASy TrEMBL
Match: A0A6J1FFL7 (uncharacterized protein LOC111445285 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445285 PE=4 SV=1)

HSP 1 Score: 680 bits (1755), Expect = 1.76e-245
Identity = 345/391 (88.24%), Postives = 362/391 (92.58%), Query Frame = 0

Query: 1   MPATDYQGSSAAW--------GVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVG 60
           MPATDYQGSSAA          +RRDQLYAM  SPTS EQDLD FQRQVA+RF++LASVG
Sbjct: 1   MPATDYQGSSAACTNIGRSVQSIRRDQLYAMDGSPTSQEQDLDSFQRQVAERFMDLASVG 60

Query: 61  ADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNA 120
            D+LLSLSWVQKLLDSFLCCQEEFK +L + KS ISK PLDRLVADYSER+VKALDVCNA
Sbjct: 61  PDELLSLSWVQKLLDSFLCCQEEFKFVLINHKSQISKPPLDRLVADYSERTVKALDVCNA 120

Query: 121 IRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSF 180
           IRDG+EQLRQWQKLLEIV+SALDNCNHQKTLGEGQFRRAKKAL DLAICMLDEKDSH S 
Sbjct: 121 IRDGIEQLRQWQKLLEIVLSALDNCNHQKTLGEGQFRRAKKALVDLAICMLDEKDSHASA 180

Query: 181 LAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240
           +AHRNRSFGRNN +KDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG
Sbjct: 181 IAHRNRSFGRNNASKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240

Query: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKR 300
           LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSL R+FPWA  ILQLHDRIVEES+KR
Sbjct: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLSRNFPWAVPILQLHDRIVEESKKR 300

Query: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLD 360
           DRRNSCGLLKEIHQIEKCTRLMNDLAD AQFPLAEEKEAELR+RVQELT VCNTLRTGLD
Sbjct: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADTAQFPLAEEKEAELRERVQELTTVCNTLRTGLD 360

Query: 361 SMERQVREVFHRIVRSRTEGLDSLGRANHSE 383
           S+ERQVREVFHR+VRSRTEGLDSLGRANHSE
Sbjct: 361 SLERQVREVFHRVVRSRTEGLDSLGRANHSE 391

BLAST of Cp4.1LG01g21330 vs. ExPASy TrEMBL
Match: A0A0A0KED5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G056490 PE=4 SV=1)

HSP 1 Score: 679 bits (1753), Expect = 3.54e-245
Identity = 348/391 (89.00%), Postives = 366/391 (93.61%), Query Frame = 0

Query: 1   MPATDYQGSSAAW--------GVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVG 60
           MPATDYQGSSAA+        G+RRDQLYAM  SPTS EQDLD FQRQVADRFL+LASVG
Sbjct: 1   MPATDYQGSSAAFTNIGRPVQGIRRDQLYAMDGSPTSPEQDLDSFQRQVADRFLDLASVG 60

Query: 61  ADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNA 120
           +DDLLSLSWV KLL+SFL CQE+FKL+L S KS IS+ PLDRLVADYSERSVKALDVCNA
Sbjct: 61  SDDLLSLSWVHKLLNSFLACQEDFKLVLISHKSQISRPPLDRLVADYSERSVKALDVCNA 120

Query: 121 IRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSF 180
           IRDG+EQLRQWQKLLEIV+SALDNCN QKTLGEGQFRRAKKAL DLAICMLDEKDSHTS 
Sbjct: 121 IRDGIEQLRQWQKLLEIVLSALDNCNSQKTLGEGQFRRAKKALIDLAICMLDEKDSHTSA 180

Query: 181 LAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240
           LAHRNRSFGRNN +KDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELL TNG
Sbjct: 181 LAHRNRSFGRNNASKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLTTNG 240

Query: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKR 300
           LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPR+FPWA+SILQLHDRIVEES+KR
Sbjct: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRNFPWASSILQLHDRIVEESKKR 300

Query: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLD 360
           DRRNSCGLLKEI+QIEKC RLMNDLAD+AQFPLAEEKEAELRQRVQELT VC+TLRTGLD
Sbjct: 301 DRRNSCGLLKEINQIEKCMRLMNDLADSAQFPLAEEKEAELRQRVQELTTVCDTLRTGLD 360

Query: 361 SMERQVREVFHRIVRSRTEGLDSLGRANHSE 383
           S+ERQVREVFHRIVRSRTEGLDSLGRANHSE
Sbjct: 361 SLERQVREVFHRIVRSRTEGLDSLGRANHSE 391

BLAST of Cp4.1LG01g21330 vs. ExPASy TrEMBL
Match: A0A6J1JWZ2 (uncharacterized protein LOC111489091 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489091 PE=4 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 1.44e-244
Identity = 344/391 (87.98%), Postives = 361/391 (92.33%), Query Frame = 0

Query: 1   MPATDYQGSSAAW--------GVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELASVG 60
           MPATDYQGSSAA          +RRDQLYAM  SPTS EQDLD FQRQVA+RF++LASVG
Sbjct: 1   MPATDYQGSSAACTNIGRSVQSIRRDQLYAMDGSPTSQEQDLDSFQRQVAERFMDLASVG 60

Query: 61  ADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNA 120
            D+LLSLSWVQKLLDSFLCCQEEFK +L + KS ISK PLDRLVADYSER+VKALDVCNA
Sbjct: 61  PDELLSLSWVQKLLDSFLCCQEEFKFVLINHKSQISKPPLDRLVADYSERTVKALDVCNA 120

Query: 121 IRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSF 180
           IRDG+EQLRQWQKLLEIV+SALDNCNHQKTLGEGQFRRAKKAL DLAICMLDEKDSH S 
Sbjct: 121 IRDGIEQLRQWQKLLEIVLSALDNCNHQKTLGEGQFRRAKKALIDLAICMLDEKDSHASA 180

Query: 181 LAHRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240
           +AHRNRSFGRNN +KDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG
Sbjct: 181 IAHRNRSFGRNNASKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNG 240

Query: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKR 300
           LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSL R+FPWA  ILQLHDRIVEES+KR
Sbjct: 241 LAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLSRNFPWAVPILQLHDRIVEESKKR 300

Query: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRTGLD 360
           DRRNSCGLLKEIHQIEKCTRLMNDLAD AQFPLAEEKEAELR+RVQELT VCNTLRTGLD
Sbjct: 301 DRRNSCGLLKEIHQIEKCTRLMNDLADTAQFPLAEEKEAELRERVQELTTVCNTLRTGLD 360

Query: 361 SMERQVREVFHRIVRSRTEGLDSLGRANHSE 383
           S+ERQVREVFHR+VRSRTEGLD LGRANHSE
Sbjct: 361 SLERQVREVFHRVVRSRTEGLDPLGRANHSE 391

BLAST of Cp4.1LG01g21330 vs. TAIR 10
Match: AT1G74450.1 (Protein of unknown function (DUF793) )

HSP 1 Score: 484.6 bits (1246), Expect = 7.5e-137
Identity = 247/397 (62.22%), Postives = 315/397 (79.35%), Query Frame = 0

Query: 1   MPATDYQGS--SAAWGVRRD------QLYAMTDSPTSHEQDLDGFQRQVADRFLELASVG 60
           MPAT+YQ S   +   +RRD      +   +T   T  E +L  FQR+VA+RF++L +  
Sbjct: 1   MPATEYQRSFGRSFLNLRRDTAVNSVESTTVTPELTQMEAELVSFQRKVAERFIDLNASS 60

Query: 61  ADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNA 120
            +DLLSL WV KLLDSFL CQEEF+ I+ + +S+I+K P+DRLV+DY ERSVKALDVCNA
Sbjct: 61  CEDLLSLEWVGKLLDSFLSCQEEFRSIVINHRSMITKPPMDRLVSDYFERSVKALDVCNA 120

Query: 121 IRDGVEQLRQWQKLLEIVVSALDN----CNHQKTLGEGQFRRAKKALTDLAICMLDEKDS 180
           IRDGVEQ+RQWQKL+EIV+ A +N     + ++ LGEGQFRRA+K L +LAI MLDEKDS
Sbjct: 121 IRDGVEQIRQWQKLIEIVICAFNNNGGGSSGKRPLGEGQFRRARKTLIELAIGMLDEKDS 180

Query: 181 HTSFLA--HRNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATE 240
            +S ++  HRNRSFGRN      R++GHFRSLSWSVSRSWSA++QLQ+IGNNLA P+A++
Sbjct: 181 SSSSVSSQHRNRSFGRNKEQLHHRTIGHFRSLSWSVSRSWSASKQLQAIGNNLATPRASD 240

Query: 241 LLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIV 300
           + ATNGL VP++TM  VLLFVMWALVAAIPCQDRGLQVHF++PR++ W  S++ LHDRI+
Sbjct: 241 ITATNGLIVPVYTMTTVLLFVMWALVAAIPCQDRGLQVHFNVPRNYQWGGSLMSLHDRII 300

Query: 301 EESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNT 360
           EES+KR+R+N+CGLLKEIHQ EK +RLMN+L D+ QFPL+EEKE E+R+RV+EL  +   
Sbjct: 301 EESKKRERKNTCGLLKEIHQFEKTSRLMNELVDSVQFPLSEEKEMEVRERVEELGKLQEA 360

Query: 361 LRTGLDSMERQVREVFHRIVRSRTEGLDSLGRANHSE 384
           L+ GLD  ER+VREVFHRIVRSRTEGLD++G+ + SE
Sbjct: 361 LKNGLDPFERKVREVFHRIVRSRTEGLDTVGKHHGSE 397

BLAST of Cp4.1LG01g21330 vs. TAIR 10
Match: AT1G18740.1 (Protein of unknown function (DUF793) )

HSP 1 Score: 467.6 bits (1202), Expect = 9.5e-132
Identity = 243/389 (62.47%), Postives = 308/389 (79.18%), Query Frame = 0

Query: 1   MPATDYQGS--SAAWGVRRDQLYAMT--------DSPTSHEQDLDGFQRQVADRFLELAS 60
           MPATD+QGS   +   +RRDQ+ + T          P++ E +LD FQRQVA++F++L +
Sbjct: 1   MPATDFQGSFGRSLLSLRRDQVDSSTVVSGSSSHHEPSTMEVELDSFQRQVAEKFIDL-N 60

Query: 61  VGADDLLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVC 120
             ++DLLSL W+ KLLDSFLCCQEEF+ I+ + +S ISK+P+DRL++DY ERS+KALDVC
Sbjct: 61  ASSNDLLSLEWIGKLLDSFLCCQEEFRAIVFNHRSQISKSPMDRLISDYFERSIKALDVC 120

Query: 121 NAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHT 180
           NAIRDG+EQ+RQW+KL +IV+SALD+    + +GEGQ RRAKKAL DLAI MLDEKD  +
Sbjct: 121 NAIRDGIEQIRQWEKLADIVISALDS---HRPIGEGQLRRAKKALIDLAIGMLDEKDHPS 180

Query: 181 -SFLAHRNRSFGRNNVTKDP--RSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATEL 240
            + LAHRNRSFGR    KD   RS+GHFRSLSWSVSRSWSA++QLQ++ +NLA P+  ++
Sbjct: 181 GTNLAHRNRSFGR---VKDSHHRSIGHFRSLSWSVSRSWSASKQLQALASNLATPRPNDV 240

Query: 241 LATNGLAVPIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVE 300
           +A+NGLAVP++TM  VLLFVMW LVAAIPCQDRGLQV+F +PR F WAA ++ LHD+IVE
Sbjct: 241 VASNGLAVPVYTMTSVLLFVMWVLVAAIPCQDRGLQVNFFVPRHFQWAAPVMSLHDKIVE 300

Query: 301 ESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTL 360
           ES++RDR+N CGLLKEI +IEK +RLMN+L D+  FPL ++KE E++QRV EL  V   L
Sbjct: 301 ESKRRDRKNCCGLLKEIDRIEKSSRLMNELIDSIHFPLNDDKEVEVKQRVDELVQVREAL 360

Query: 361 RTGLDSMERQVREVFHRIVRSRTEGLDSL 377
           R GLD  ER+VREVFHRIVRSRTE LDSL
Sbjct: 361 RNGLDPFERKVREVFHRIVRSRTESLDSL 382

BLAST of Cp4.1LG01g21330 vs. TAIR 10
Match: AT1G43630.1 (Protein of unknown function (DUF793) )

HSP 1 Score: 434.1 bits (1115), Expect = 1.2e-121
Identity = 222/390 (56.92%), Postives = 294/390 (75.38%), Query Frame = 0

Query: 1   MPATDYQGSSAAWGVRRDQLYAMT----DSPTSHEQDLDGFQRQVADRFLEL-ASVGADD 60
           MP T+Y    +   +RRDQ + M       P + E +LD FQRQVA++F++L AS    +
Sbjct: 31  MPETEYSFGRSFLSLRRDQAHLMDPTSFSEPMTMEVELDSFQRQVAEKFIDLNASADEAE 90

Query: 61  LLSLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRD 120
           +LSL W+ KLLDSFLCCQE+F++I+ + K  + K P+DRL+ +Y ERSVKALDVCNAIRD
Sbjct: 91  ILSLEWIGKLLDSFLCCQEDFRVIIFNHKPQLLKQPMDRLIEEYFERSVKALDVCNAIRD 150

Query: 121 GVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAH 180
           G+EQ+RQWQKL+EIV+SALD   +Q+ LGEG+  RAKKAL DLAI MLDEKDS  +   H
Sbjct: 151 GIEQIRQWQKLIEIVISALD--TNQRQLGEGEIHRAKKALIDLAIGMLDEKDSSNT---H 210

Query: 181 RNRSFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLATNGLAV 240
           RNRSF RN      + +G+ RSLSWSVSRSWSA+RQLQ IGNNLA P+A++++ATNGLA+
Sbjct: 211 RNRSFTRNK--DHNQHIGYIRSLSWSVSRSWSASRQLQGIGNNLATPRASDVMATNGLAL 270

Query: 241 PIFTMNMVLLFVMWALVAAIPCQDRGLQVHFSLPRSFPWAASILQLHDRIVEESRKRD-R 300
            ++TM  +LLFV W LVAAIPCQDRGL VHF  PR F WA  ++ LHD+I++ES+KRD +
Sbjct: 271 TVYTMTSILLFVTWVLVAAIPCQDRGLHVHFYFPRHFQWAVPVMSLHDKIMDESKKRDKK 330

Query: 301 RNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEK-EAELRQRVQELTGVCNTLRTGLDS 360
           +  CGLL+EI+QIE+ +R+++DL D+  F L +EK   E+++RVQEL  VC  ++ GLD 
Sbjct: 331 KKGCGLLREINQIERNSRMLSDLIDSDNFSLTDEKCTLEVKERVQELMNVCEAIKEGLDP 390

Query: 361 MERQVREVFHRIVRSRTEGLDSLGRANHSE 384
            +R+VR+VFH+IVR+RTE LDSLG+  + E
Sbjct: 391 FDRKVRDVFHQIVRTRTEALDSLGKLQNQE 413

BLAST of Cp4.1LG01g21330 vs. TAIR 10
Match: AT1G63930.1 (from the Czech 'roh' meaning 'corner' )

HSP 1 Score: 260.4 bits (664), Expect = 2.3e-69
Identity = 152/414 (36.71%), Postives = 250/414 (60.39%), Query Frame = 0

Query: 2   PATDYQGS-SAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLEL------------ 61
           PA D QGS      +RR+Q   + +     ++DL+ FQ+ +ADRF EL            
Sbjct: 3   PAQDNQGSFLGRISIRRNQFVDVNNE--QEQEDLELFQKHIADRFTELLSPPQPPPSDEI 62

Query: 62  ---ASVGA-DDLLSLSWVQKLLDSFLCCQEEFK--LILDSQKSLISKAPLDRLVADYSER 121
              ASV A + ++S++W++KL+D FLCC+ EFK  L++    + ISK P DRLV +  +R
Sbjct: 63  NTVASVAATEQIMSVTWLRKLMDVFLCCEAEFKAILLMGRDPTQISKPPFDRLVPEMLDR 122

Query: 122 SVKALDVCNAIRDGVEQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICM 181
           S+KALD+C A+ +G++ +R +Q+L EI V+AL+    Q+ LG+G  RRAK+AL +L + +
Sbjct: 123 SIKALDICTAVVNGIDSVRHYQRLAEIAVTALE----QRPLGDGNVRRAKRALANLVVAL 182

Query: 182 -LDEKDSHT---------SFLAHRNRSFGRNN-----VTKDPRSLGHFRSLSWSVSRSWS 241
            L++K++ +         +    R+ SFGR +      +K   ++G  +S SW+V R+WS
Sbjct: 183 SLEDKENVSGGGGGGGGGNKTTERSWSFGRRSGGSSAASKGGATIGQLKSSSWAVGRNWS 242

Query: 242 AARQLQSIGNNLAAPKATELLATNGLAVPIFTMNMVLLFVMWALVAAIPCQDR-GLQVHF 301
           AA+Q+ ++  NL  P+  E     GL  P+F M+ V++FVMW L AA+PCQ+R GL  H 
Sbjct: 243 AAKQIHAMTANLTPPRGNE---AAGLPQPMFIMSTVMVFVMWVLTAAVPCQERSGLANHL 302

Query: 302 SL-PRSFPWAASILQLHDRIVEESRKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPL 361
            + P+   WA S++ +H++I +E +K++++ S GL++E+ ++EK    + + AD   +P 
Sbjct: 303 PVPPKHLNWAQSLIGIHEKIGDEWKKKEKKGSAGLMEEMTRMEKLGHSLMEFADGFHYPA 362

Query: 362 AEEKEAELRQRVQELTGVCNTLRTGLDSMERQVREVFHRIVRSRTEGLDSLGRA 380
            ++       +V E+  +C  +   L  +++Q+REVFHRIVRSR E L+ L +A
Sbjct: 363 EKDAAESAAVQVAEMAEICRRMEEELVPLQQQIREVFHRIVRSRAEILEVLEQA 407

BLAST of Cp4.1LG01g21330 vs. TAIR 10
Match: AT4G11300.1 (Protein of unknown function (DUF793) )

HSP 1 Score: 231.1 bits (588), Expect = 1.5e-60
Identity = 143/385 (37.14%), Postives = 229/385 (59.48%), Query Frame = 0

Query: 2   PATDYQGSSAAWGVRRDQLYAMTDSPTSHEQDLDGFQRQVADRFLELA----SVGADDLL 61
           PAT++Q S  +   RR+Q+ +M  +    +++L+ FQ+ VA+RF EL     S  +  +L
Sbjct: 3   PATEHQSSFLSRLSRRNQVVSMEVNHEQEQEELEDFQKHVAERFAELLPPSDSPESYPIL 62

Query: 62  SLSWVQKLLDSFLCCQEEFKLILDSQKSLISKAPLDRLVADYSERSVKALDVCNAIRDGV 121
           S+ W++KLLD F+  + EF  +L S  S ISK PLD+LV +  +R VKALD+C A+ +GV
Sbjct: 63  SIQWLRKLLDVFMSIESEFHSVLTSNPSQISKPPLDKLVPEMLDRIVKALDICTAVVNGV 122

Query: 122 EQLRQWQKLLEIVVSALDNCNHQKTLGEGQFRRAKKALTDLAICMLDEKDSHTSFLAHRN 181
           + +RQ Q+  EI V+AL     Q  L +G  RRAK+ALT L   +  +K+S +S      
Sbjct: 123 DSVRQIQRCAEIAVTAL----KQTPLSDGSVRRAKRALTSLLAALNADKNSGSSGGGSGR 182

Query: 182 R-------SFGRNNVTKDPRSLGHFRSLSWSVSRSWSAARQLQSIGNNLAAPKATELLAT 241
           R       SFGR    +   S G     +  VS++WSAA+Q+Q++  NL AP+       
Sbjct: 183 RSSTDQWSSFGR----RSGGSSGGGGGGAGCVSKNWSAAKQIQAMTANLVAPR------- 242

Query: 242 NGLAVPIFTMNMVLLFVMWALVAAIPCQ-DRGLQVHFSLPRSFPWAASILQLHDRIVEES 301
            G A P++ M+ V++ VMW LV A+PCQ   GL VH  LP++  WA + + + +R+ EE 
Sbjct: 243 GGEASPMYIMSSVMVMVMWTLVVAVPCQTSNGLMVHVPLPKNQVWANAAVSISERVGEEM 302

Query: 302 RKRDRRNSCGLLKEIHQIEKCTRLMNDLADAAQFPLAEEKEAELRQRVQELTGVCNTLRT 361
           ++++ R   GL++E+ ++E+    + + ++  +F   E+  AE    V E+  +C  +  
Sbjct: 303 KRKETRGG-GLMEEMQRMERIGLKLMEFSEGFRFNGEEDVVAE----VAEMEEICRKMED 362

Query: 362 GLDSMERQVREVFHRIVRSRTEGLD 375
           GL+ ++R+VREVFHR+V+SR+E L+
Sbjct: 363 GLEGLQRRVREVFHRLVKSRSEILE 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9CAK43.2e-6836.71Protein ROH1 OS=Arabidopsis thaliana OX=3702 GN=ROH1 PE=1 SV=1[more]
Q337C06.4e-0822.04UPF0496 protein 4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0513300 PE=2 ... [more]
A2Z9A68.4e-0822.37UPF0496 protein 4 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_033149 PE=3 SV=2[more]
Q9LMM61.3e-0519.53Protein BPS1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BPS1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023528071.17.34e-275100.00UPF0496 protein 4-like [Cucurbita pepo subsp. pepo][more]
XP_022963767.14.07e-27299.22UPF0496 protein 4-like [Cucurbita moschata] >KAG7032872.1 hypothetical protein S... [more]
XP_022990383.11.59e-26998.43UPF0496 protein 4-like [Cucurbita maxima][more]
KAG6602188.14.93e-25297.79Protein ROH1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022939341.13.63e-24588.24uncharacterized protein LOC111445285 isoform X1 [Cucurbita moschata] >XP_0229393... [more]
Match NameE-valueIdentityDescription
A0A6J1HG411.97e-27299.22UPF0496 protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111463959 PE=4 SV=1[more]
A0A6J1JT467.68e-27098.43UPF0496 protein 4-like OS=Cucurbita maxima OX=3661 GN=LOC111487256 PE=4 SV=1[more]
A0A6J1FFL71.76e-24588.24uncharacterized protein LOC111445285 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0KED53.54e-24589.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G056490 PE=4 SV=1[more]
A0A6J1JWZ21.44e-24487.98uncharacterized protein LOC111489091 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G74450.17.5e-13762.22Protein of unknown function (DUF793) [more]
AT1G18740.19.5e-13262.47Protein of unknown function (DUF793) [more]
AT1G43630.11.2e-12156.92Protein of unknown function (DUF793) [more]
AT1G63930.12.3e-6936.71from the Czech 'roh' meaning 'corner' [more]
AT4G11300.11.5e-6037.14Protein of unknown function (DUF793) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008511Protein BYPASS-relatedPFAMPF05633BPS1coord: 1..376
e-value: 6.1E-163
score: 542.3
NoneNo IPR availablePANTHERPTHR31509:SF0OS04G0688000 PROTEINcoord: 13..381
NoneNo IPR availablePANTHERPTHR31509BPS1-LIKE PROTEINcoord: 13..381

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g21330.1Cp4.1LG01g21330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane