Cp4.1LG01g20050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g20050 vs. ExPASy Swiss-Prot
Match: Q9LT31 (Vacuolar protein sorting-associated protein 9A OS=Arabidopsis thaliana OX=3702 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.2e-143
Identity = 285/443 (64.33%), Postives = 338/443 (76.30%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKL++RV+AS  ++V  DE+L  KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDI+P FQNESSWLLAQKEL KINMYKAPRDKLVC+L CCKVI+NLLLNASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DA ++S++E EFEKNMESARA +SGL +    + + S    R     E     +Q+ N  
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPR----DESTLQKTQSLNPK 300

Query: 301 TEPAV-RPNSPESKSST-EGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYP 360
            E  + +  S +S S T E  ++  ++ + +  ++SDLENKGA  LL D    + F++YP
Sbjct: 301 RENTLFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQEYP 360

Query: 361 YLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSS-SPSSSNSEMHSHHNPESFK 420
           Y+FA  GDL   DVE LLN Y+ LVFKYV L+KGL   +S +PSSS  +  S  N  + K
Sbjct: 361 YIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGTSLAPSSSPLQASSGFN--TSK 420

Query: 421 EPEDVRDVTSNDESAGETHTMID 441
           E ED R  +S+ +   ET   +D
Sbjct: 421 ESEDHRRSSSDVQMTKETDRSVD 436

BLAST of Cp4.1LG01g20050 vs. ExPASy Swiss-Prot
Match: Q10NQ3 (Vacuolar protein sorting-associated protein 9A OS=Oryza sativa subsp. japonica OX=39947 GN=VPS9A PE=1 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 4.7e-133
Identity = 256/428 (59.81%), Postives = 316/428 (73.83%), Query Frame = 0

Query: 8   LGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLW 67
           L  HDFLERMRQPSA+DFVKSIK FIV+FSN APDPE DSA+VQEF   MEGAFRAH  W
Sbjct: 17  LAWHDFLERMRQPSAADFVKSIKGFIVTFSNRAPDPEHDSAAVQEFLENMEGAFRAHTPW 76

Query: 68  SGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIRPENLDIK 127
           +G SEEELESAGEGLEKYVMTKL++RV+AS+ +DVK DE+L +KM+L+QQFIRPENLDIK
Sbjct: 77  AGSSEEELESAGEGLEKYVMTKLFNRVFASVPEDVKSDEELFEKMSLLQQFIRPENLDIK 136

Query: 128 PNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNENPPGADEF 187
           P +Q+E+SWLLAQKEL KINMYKAPRDKL C+L CCKVI+NLLLNASI SNENPPGADEF
Sbjct: 137 PEYQSETSWLLAQKELQKINMYKAPRDKLACILNCCKVINNLLLNASIVSNENPPGADEF 196

Query: 188 LPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSM 247
           LPVLIYV IKANPPQLHSNLLYIQRYR QSRL  EA YFFTNILSAESFI NID  +LSM
Sbjct: 197 LPVLIYVTIKANPPQLHSNLLYIQRYRRQSRLVSEAQYFFTNILSAESFIWNIDGESLSM 256

Query: 248 EETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSGTEPAVRP 307
           +E +F+K M+ AR  + GLS   E+  N++NLD R  ++  L  +     N   +   + 
Sbjct: 257 DERDFQKKMDLARERMLGLSASSENQDNQNNLDVREQKSQTLKASRDSDVNLSLKDNFQG 316

Query: 308 NSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYLFAQVGDL 367
              E +  ++       + V  V ++SDLE KGAA LL D    +  ++YP+LFA+ GDL
Sbjct: 317 PGLEMRRDSDAS----SNPVERVQSISDLEKKGAAELLKDDDLNKKIQEYPFLFARSGDL 376

Query: 368 TFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPEDVRDVTS 427
           T  DVE LLN Y+ LV KYV+LS+G+ +   +P   +  M +  +    +EP++V++  +
Sbjct: 377 TVADVENLLNSYKQLVLKYVALSQGMGINLENPPVQS--MQTVSDLVESEEPKNVKNAVN 436

Query: 428 NDESAGET 436
             E + +T
Sbjct: 437 FSEGSSKT 438

BLAST of Cp4.1LG01g20050 vs. ExPASy Swiss-Prot
Match: O74396 (Vacuolar protein sorting-associated protein 9a OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=vps901 PE=1 SV=3)

HSP 1 Score: 163.7 bits (413), Expect = 5.1e-39
Identity = 104/313 (33.23%), Postives = 167/313 (53.35%), Query Frame = 0

Query: 11  HDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGC 70
           H FLE++R  SA    K +KSF+  F+            +++F   +      +  W+  
Sbjct: 116 HMFLEQLRSSSAEPVAKYLKSFLSEFTKRRWTVNYQVKLIRDFLKFINEKIEQYEPWASG 175

Query: 71  SEEELESAGEGLEKYVMTKLYSRVY------------ASLADDVKIDEQLSDKMALIQQF 130
           S+ E+++A EG+EK V+ +LY+ ++            +  +DDV+ D  LS+KM L  Q+
Sbjct: 176 SQAEIDNAKEGMEKLVLNRLYTSLFSPEIAKSGIPLSSEHSDDVEEDRVLSEKMELF-QW 235

Query: 131 IRPENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASN 190
           I  ENLDIK    +   + LA  EL +IN Y APRDK++CLL CCKVI + L N  +   
Sbjct: 236 ITEENLDIKKQKSSSKFFKLAADELRRINDYHAPRDKIICLLNCCKVIFSYLRN--VVKE 295

Query: 191 ENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFIS 250
           E+   AD F+P+LI+V+++A P  L SN+ YIQR+R   +LTGE  Y+ + ++ A SFI 
Sbjct: 296 ES---ADMFVPILIFVVLQARPAHLVSNIQYIQRFRSPEKLTGEVMYYLSTLMGAMSFIE 355

Query: 251 NIDANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASN 310
            +D ++L++ E EF   +E +   +       E S +K+ ++E       ++     +S 
Sbjct: 356 TLDCSSLTITEEEFNAQIEKSIKKMEERKLS-EKSESKTAVNENATYKDPVLSRGLSSSI 415

Query: 311 SGTEPAVRPNSPE 312
             +      N PE
Sbjct: 416 DVSTGVALVNLPE 421

BLAST of Cp4.1LG01g20050 vs. ExPASy Swiss-Prot
Match: P54787 (Vacuolar protein sorting-associated protein 9 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=VPS9 PE=1 SV=2)

HSP 1 Score: 120.2 bits (300), Expect = 6.5e-26
Identity = 97/313 (30.99%), Postives = 158/313 (50.48%), Query Frame = 0

Query: 7   FLGLHDFLERMRQPSASDFVKSIKSFIVSF--SNNAPDPERDSASVQEFFAKMEGAFRAH 66
           F     F+++++ P A   VK  KSF+ +F           +   + +F   +   F  +
Sbjct: 60  FYDFQIFIKQLQTPGADPLVKYTKSFLRNFLAQRLLWTVSEEIKLISDFKTFIYDKFTLY 119

Query: 67  PLWSGCSEEELESAGEGLEKYVMTKLYSRVYA-SLAD------------DVKIDEQLSDK 126
             +      ++ +A EG+EK +M KLYSR ++ SL +            D+  D+ L +K
Sbjct: 120 EPFRSLDNSKMRNAKEGMEKLIMGKLYSRCFSPSLYEILQKPLDDEHMKDLTNDDTLLEK 179

Query: 127 MALIQQFIRPENLDI---KPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISN 186
           +    +FI P  LDI    PN +      LA KEL KIN +K+PRDK+VC+L   KVI  
Sbjct: 180 IR-HYRFISPIMLDIPDTMPNARLNKFVHLASKELGKINRFKSPRDKMVCVLNASKVIFG 239

Query: 187 LLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFT 246
           LL +  +  N    GAD F+PVLIY I+K     L SN+ YI+R+R    + GE  Y+ +
Sbjct: 240 LLKHTKLEQN----GADSFIPVLIYCILKGQVRYLVSNVNYIERFRSPDFIRGEEEYYLS 299

Query: 247 NILSAESFISNIDANALSMEETE-FEKNMESARALLSGLSTDVESSSNKSNLD--ERVAR 299
           ++ +A +FI N+   +L++E+ E FE   E+ +     L+ + E    K  L+  + +  
Sbjct: 300 SLQAALNFIMNLTERSLTIEDHEDFE---EAYQRNFKQLAEEKEEEEKKKQLEIPDELQP 359

BLAST of Cp4.1LG01g20050 vs. ExPASy Swiss-Prot
Match: O18973 (Rab5 GDP/GTP exchange factor OS=Bos taurus OX=9913 GN=RABGEF1 PE=1 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.5e-25
Identity = 74/218 (33.94%), Postives = 122/218 (55.96%), Query Frame = 0

Query: 44  ERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYA-SLADDV 103
           E  S   Q+F+  +    +          E +E   + +EKY+MT+LY  V+     DD 
Sbjct: 177 EEQSECTQDFYQNVAERMQTR---GKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDE 236

Query: 104 KIDEQLSDKMALIQQFIRPENLDIKPNFQ-NESSWLL--AQKELLKINMYKAPRDKLVCL 163
           K D  +  ++  +  ++ P+ L +  N +  E S ++  A  ++++++  + PRDKL C+
Sbjct: 237 KKDLAIQKRIRAL-HWVTPQMLCVPVNEEIPEVSDMVVKAITDIIEMDSKRVPRDKLACI 296

Query: 164 LGCCKVISNLLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRL 223
             C K I N +    I  NE P  AD+FLP LIY+++K NPP+L SN+ YI R+   SRL
Sbjct: 297 TKCSKHIFNAI---KITKNE-PASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRL 356

Query: 224 -TGEAAYFFTNILSAESFISNIDANALSMEETEFEKNM 257
            TGE  Y+FTN+  A +FI  +DA +L++ + +F++ M
Sbjct: 357 MTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYM 386

BLAST of Cp4.1LG01g20050 vs. NCBI nr
Match: XP_023525567.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Cp4.1LG01g20050 vs. NCBI nr
Match: XP_022955656.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata] >XP_022955669.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita moschata])

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 472/476 (99.16%), Postives = 472/476 (99.16%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAVRPNSPESK STEGPHVKYQSTV EVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSH NPESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHE NDTTSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVNDTTSLS 476

BLAST of Cp4.1LG01g20050 vs. NCBI nr
Match: XP_022990551.1 (vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima] >XP_022990552.1 vacuolar protein sorting-associated protein 9A-like [Cucurbita maxima])

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 468/476 (98.32%), Postives = 472/476 (99.16%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERD ASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DAN+LSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAVRPNSPESK STEGPHVKYQSTVMEVSTLSDLE+KGAAMLLNDHTRKQAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDVEELLNQYQH+VFKYVSLSKGLSLRSSSPSSS+SEMHSHHNPESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           DVRDVTSNDESAGETHTMIDGPDGTS FGEENVGSGLMQDKVVLPQHEGNDTTSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Cp4.1LG01g20050 vs. NCBI nr
Match: KAG7032750.1 (Vacuolar protein sorting-associated protein 9A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 890 bits (2301), Expect = 0.0
Identity = 470/500 (94.00%), Postives = 470/500 (94.00%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKS------------------------FIVSF 60
           MENADVFLGLHDFLERMRQPSASDFVKSIK                         FIVSF
Sbjct: 24  MENADVFLGLHDFLERMRQPSASDFVKSIKRCAYLYVYALVCRVSDRYAFCSLILFIVSF 83

Query: 61  SNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYA 120
           SNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYA
Sbjct: 84  SNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYA 143

Query: 121 SLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKL 180
           SLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKL
Sbjct: 144 SLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKL 203

Query: 181 VCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ 240
           VCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ
Sbjct: 204 VCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ 263

Query: 241 SRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNMESARALLSGLSTDVESSSNK 300
           SRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNMESARALLSGLSTDVESSSNK
Sbjct: 264 SRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNMESARALLSGLSTDVESSSNK 323

Query: 301 SNLDERVARNSELVETSSQASNSGTEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDL 360
           SNLDERVA NSELVETSSQASNSGTEPAVRPNSPESK STEGPHVKYQSTV EVSTLSDL
Sbjct: 324 SNLDERVACNSELVETSSQASNSGTEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDL 383

Query: 361 ENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLR 420
           ENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLR
Sbjct: 384 ENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLR 443

Query: 421 SSSPSSSNSEMHSHHNPESFKEPEDVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSG 476
           SSSPSSSNSEMHSH NPESFKEPEDVRDVTSNDESAGETHTMIDGPDGTS FGEENVGSG
Sbjct: 444 SSSPSSSNSEMHSHLNPESFKEPEDVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSG 503

BLAST of Cp4.1LG01g20050 vs. NCBI nr
Match: KAG6602057.1 (Vacuolar protein sorting-associated protein 9A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 846 bits (2185), Expect = 2.12e-307
Identity = 440/445 (98.88%), Postives = 441/445 (99.10%), Query Frame = 0

Query: 17  MRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGAFRAHPLWSGCSEEELE 76
           MRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQ+FFAKMEGAFRAHPLWSGCSEEELE
Sbjct: 1   MRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQDFFAKMEGAFRAHPLWSGCSEEELE 60

Query: 77  SAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSW 136
           SAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSW
Sbjct: 61  SAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIRPENLDIKPNFQNESSW 120

Query: 137 LLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVII 196
           LLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVII
Sbjct: 121 LLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVII 180

Query: 197 KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNM 256
           KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNM
Sbjct: 181 KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNM 240

Query: 257 ESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSGTEPAVRPNSPESKSST 316
           ESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSGTEPAVRPNSPESK ST
Sbjct: 241 ESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSGTEPAVRPNSPESKPST 300

Query: 317 EGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELL 376
           EGPHVKYQSTV EVST SDLENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELL
Sbjct: 301 EGPHVKYQSTVTEVSTHSDLENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEELL 360

Query: 377 NQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPEDVRDVTSNDESAGETH 436
           NQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPEDVRDVTSNDESAGETH
Sbjct: 361 NQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPEDVRDVTSNDESAGETH 420

Query: 437 TMIDGPDGTSPFGEENVGSGLMQDK 461
           TMIDGPDGTS FGEENVGSGLMQDK
Sbjct: 421 TMIDGPDGTSLFGEENVGSGLMQDK 445

BLAST of Cp4.1LG01g20050 vs. ExPASy TrEMBL
Match: A0A6J1GWY3 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita moschata OX=3662 GN=LOC111457590 PE=4 SV=1)

HSP 1 Score: 909 bits (2350), Expect = 0.0
Identity = 472/476 (99.16%), Postives = 472/476 (99.16%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG
Sbjct: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAVRPNSPESK STEGPHVKYQSTV EVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVTEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSH NPESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHLNPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHE NDTTSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEVNDTTSLS 476

BLAST of Cp4.1LG01g20050 vs. ExPASy TrEMBL
Match: A0A6J1JJ31 (vacuolar protein sorting-associated protein 9A-like OS=Cucurbita maxima OX=3661 GN=LOC111487398 PE=4 SV=1)

HSP 1 Score: 905 bits (2338), Expect = 0.0
Identity = 468/476 (98.32%), Postives = 472/476 (99.16%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERD ASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDGASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN
Sbjct: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 181 SPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DAN+LSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG
Sbjct: 241 DANSLSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAVRPNSPESK STEGPHVKYQSTVMEVSTLSDLE+KGAAMLLNDHTRKQAFRDYPYL
Sbjct: 301 TEPAVRPNSPESKPSTEGPHVKYQSTVMEVSTLSDLESKGAAMLLNDHTRKQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDVEELLNQYQH+VFKYVSLSKGLSLRSSSPSSS+SEMHSHHNPESFKEPE
Sbjct: 361 FAQVGDLTFNDVEELLNQYQHVVFKYVSLSKGLSLRSSSPSSSSSEMHSHHNPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           DVRDVTSNDESAGETHTMIDGPDGTS FGEENVGSGLMQDKVVLPQHEGNDTTSLS
Sbjct: 421 DVRDVTSNDESAGETHTMIDGPDGTSLFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476

BLAST of Cp4.1LG01g20050 vs. ExPASy TrEMBL
Match: A0A6J1CKF0 (vacuolar protein sorting-associated protein 9A-like OS=Momordica charantia OX=3673 GN=LOC111011952 PE=4 SV=1)

HSP 1 Score: 783 bits (2022), Expect = 3.63e-283
Identity = 407/471 (86.41%), Postives = 432/471 (91.72%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL++RVYASL DDVKIDEQLSDK+ALIQQF R
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFTRVYASLRDDVKIDEQLSDKIALIQQFAR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINMYKAPRDKLVCLL CCKVISNLL+NAS+ASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMYKAPRDKLVCLLNCCKVISNLLINASVASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQ+RLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQNRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DA ALSM+E EFE  MESARALLSGLSTD E+ SNKSNLDE V RNS LV++SSQASN G
Sbjct: 241 DAKALSMDEIEFENKMESARALLSGLSTDAEAISNKSNLDEGVVRNSGLVKSSSQASNVG 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYL 360
           TEPAV P S E+K  TE    K QSTVME  +LSDLENKGAA+LLNDHT +QAFRDYPYL
Sbjct: 301 TEPAVIPKSAEAKPRTEASCAKDQSTVMEEPSLSDLENKGAAILLNDHTGRQAFRDYPYL 360

Query: 361 FAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEPE 420
           FAQVGDLTFNDV+ELL+QY+ LVFKY+SLSKGLSL +SS S S+SEM SHH PESFKEPE
Sbjct: 361 FAQVGDLTFNDVDELLSQYKQLVFKYISLSKGLSLTTSSVSVSHSEMQSHHRPESFKEPE 420

Query: 421 DVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGND 471
            +RDVTSN+ESAGETH  IDGPDG S FGEE+VGSGL QDKVVL QHEGND
Sbjct: 421 GIRDVTSNEESAGETHKRIDGPDGISLFGEESVGSGLPQDKVVLLQHEGND 471

BLAST of Cp4.1LG01g20050 vs. ExPASy TrEMBL
Match: A0A1S3B4M4 (vacuolar protein sorting-associated protein 9A-like OS=Cucumis melo OX=3656 GN=LOC103485761 PE=4 SV=1)

HSP 1 Score: 766 bits (1978), Expect = 4.43e-276
Identity = 408/477 (85.53%), Postives = 428/477 (89.73%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 29  MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 88

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL+SRVYASLADDVKIDEQLS+KMALIQQFIR
Sbjct: 89  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQLSEKMALIQQFIR 148

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINM+KAPRDKLVCLL CCKVISNLL NASIASNEN
Sbjct: 149 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 208

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI
Sbjct: 209 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 268

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DA ALSMEE EFEKNMESARALLSGLS+DVE+ SNK+NLDE V   SELVETSSQAS   
Sbjct: 269 DAKALSMEEIEFEKNMESARALLSGLSSDVEAISNKNNLDEGVGHYSELVETSSQAS--- 328

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTR-KQAFRDYPY 360
            E  VRP   E K  TE PH K QSTVMEV +LSDLENKGA +LLND T  +QA RDYPY
Sbjct: 329 VESQVRPKPTEGKPRTEAPHAKDQSTVMEVPSLSDLENKGATILLNDQTGGRQALRDYPY 388

Query: 361 LFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEP 420
           LF+QVGDLTF DVEELL+QY+ LVFKYVSLS+GL L +   S SNSEM+S H+ ESFKEP
Sbjct: 389 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLGLTTELSSYSNSEMYSQHHHESFKEP 448

Query: 421 EDVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           EDVRD+TSNDESAGE H +IDGPDGTS FGEENVGSGL  DKVVLPQHEGN+  S S
Sbjct: 449 EDVRDMTSNDESAGEGHRLIDGPDGTSLFGEENVGSGL--DKVVLPQHEGNNEMSQS 500

BLAST of Cp4.1LG01g20050 vs. ExPASy TrEMBL
Match: A0A0A0LI50 (VPS9 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G128630 PE=4 SV=1)

HSP 1 Score: 763 bits (1969), Expect = 3.96e-275
Identity = 403/477 (84.49%), Postives = 426/477 (89.31%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA
Sbjct: 1   MENTDVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEELESAGEGLEKYVMTKL+SRVYASLADDVKIDEQ+S+KMALIQQFIR
Sbjct: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLFSRVYASLADDVKIDEQISEKMALIQQFIR 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDIKPNFQNE+SWLLAQKELLKINM+KAPRDKLVCLL CCKVISNLL NASIASNEN
Sbjct: 121 PENLDIKPNFQNETSWLLAQKELLKINMHKAPRDKLVCLLSCCKVISNLLFNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
           PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTN+LSAESFISNI
Sbjct: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNVLSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DA ALSMEE EFEKNMESARALLSGLS+DVE+ SNK+NLDE V   SELVETSSQAS   
Sbjct: 241 DAKALSMEEIEFEKNMESARALLSGLSSDVEALSNKNNLDEGVGHYSELVETSSQAS--- 300

Query: 301 TEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTR-KQAFRDYPY 360
            E  VRP   E K  TE PH K QS VM+V +LSDLENKGA +LLND T  +QA R+YPY
Sbjct: 301 VESPVRPKPTEGKPRTEAPHAKDQSAVMKVPSLSDLENKGATILLNDQTGGRQALREYPY 360

Query: 361 LFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSSSPSSSNSEMHSHHNPESFKEP 420
           LF+QVGDLTF DVEELL+QY+ LVFKYVSLS+GLSL +   S S SEMHS H+ ESFKEP
Sbjct: 361 LFSQVGDLTFKDVEELLSQYKQLVFKYVSLSRGLSLTTEFSSYSKSEMHSQHHHESFKEP 420

Query: 421 EDVRDVTSNDESAGETHTMIDGPDGTSPFGEENVGSGLMQDKVVLPQHEGNDTTSLS 476
           EDVRDVTSNDESAG+ H +IDGPDGTS FGEENVGSGL QDKV LPQ EGN+  S S
Sbjct: 421 EDVRDVTSNDESAGDRHRLIDGPDGTSLFGEENVGSGLPQDKVELPQREGNNEMSQS 474

BLAST of Cp4.1LG01g20050 vs. TAIR 10
Match: AT3G19770.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 510.4 bits (1313), Expect = 1.6e-144
Identity = 285/443 (64.33%), Postives = 338/443 (76.30%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DVFLGLHDFLERMR+PSA DFVKSIKSFIVSFSNNAPDPE+D A VQEFF+KME A
Sbjct: 1   MENTDVFLGLHDFLERMRKPSAGDFVKSIKSFIVSFSNNAPDPEKDCAMVQEFFSKMEAA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCSEEEL+SAG+GLEKYVMTKL++RV+AS  ++V  DE+L  KM+L+QQFI 
Sbjct: 61  FRAHPLWSGCSEEELDSAGDGLEKYVMTKLFTRVFASNTEEVIADEKLFQKMSLVQQFIS 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDI+P FQNESSWLLAQKEL KINMYKAPRDKLVC+L CCKVI+NLLLNASIASNEN
Sbjct: 121 PENLDIQPTFQNESSWLLAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNEN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGADEFLPVLIYV IKANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNI
Sbjct: 181 APGADEFLPVLIYVTIKANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSG 300
           DA ++S++E EFEKNMESARA +SGL +    + + S    R     E     +Q+ N  
Sbjct: 241 DAKSISLDEAEFEKNMESARARISGLDSQTYQTGHGSAPPPR----DESTLQKTQSLNPK 300

Query: 301 TEPAV-RPNSPESKSST-EGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYP 360
            E  + +  S +S S T E  ++  ++ + +  ++SDLENKGA  LL D    + F++YP
Sbjct: 301 RENTLFQSKSSDSLSGTNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQEYP 360

Query: 361 YLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSS-SPSSSNSEMHSHHNPESFK 420
           Y+FA  GDL   DVE LLN Y+ LVFKYV L+KGL   +S +PSSS  +  S  N  + K
Sbjct: 361 YIFASAGDLRIGDVEGLLNSYKQLVFKYVCLTKGLGDGTSLAPSSSPLQASSGFN--TSK 420

Query: 421 EPEDVRDVTSNDESAGETHTMID 441
           E ED R  +S+ +   ET   +D
Sbjct: 421 ESEDHRRSSSDVQMTKETDRSVD 436

BLAST of Cp4.1LG01g20050 vs. TAIR 10
Match: AT5G09320.1 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 454.9 bits (1169), Expect = 7.9e-128
Identity = 247/418 (59.09%), Postives = 302/418 (72.25%), Query Frame = 0

Query: 1   MENADVFLGLHDFLERMRQPSASDFVKSIKSFIVSFSNNAPDPERDSASVQEFFAKMEGA 60
           MEN DV  GLH+FL +   PSA DF+KSIKSFIVS  N APDPE+D  +VQ+FF KME A
Sbjct: 1   MENTDVVSGLHNFLSK---PSAKDFIKSIKSFIVSILNTAPDPEKDCDAVQDFFYKMESA 60

Query: 61  FRAHPLWSGCSEEELESAGEGLEKYVMTKLYSRVYASLADDVKIDEQLSDKMALIQQFIR 120
           FRAHPLWSGCS++EL++AG+GLEKYVMTKL+ RV+AS  +DV  DE+L  K++L+QQFI 
Sbjct: 61  FRAHPLWSGCSDDELDNAGDGLEKYVMTKLFPRVFASNTEDVISDEKLFQKISLVQQFIS 120

Query: 121 PENLDIKPNFQNESSWLLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNEN 180
           PENLDI+P FQN++SWLLAQKEL KINMY APRDKL+C+L CCKVI+NLLLNASIASN+N
Sbjct: 121 PENLDIQPTFQNQTSWLLAQKELQKINMYNAPRDKLMCILRCCKVINNLLLNASIASNQN 180

Query: 181 PPGADEFLPVLIYVIIKANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNI 240
            PGAD+FLPVLIYV IKANPPQ HSNLLYIQRYR QS+L GEA Y FTNILSAESFISNI
Sbjct: 181 EPGADQFLPVLIYVTIKANPPQFHSNLLYIQRYRRQSKLVGEAGYLFTNILSAESFISNI 240

Query: 241 DANALSMEETEFEKNMESARALLSGLSTDVESSSNKSNLD--ERVARNSELVETSSQASN 300
           DA +LSM+E +FE  M+SA A LSG  +    + + + L       R + L+ T S  S 
Sbjct: 241 DAKSLSMDEADFETKMKSAHARLSGPGSQSYQTDHGAALPTAHNTKRENMLLHTKSTDSF 300

Query: 301 SGTEPAVRPNSPESKSSTEGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTR-KQAFRDY 360
           SGT   +                  ++ + +   ++DLENKGAA LLND +   + F++Y
Sbjct: 301 SGTNETLS-----------------ETPIKKADPITDLENKGAATLLNDRSEATKIFQEY 360

Query: 361 PYLFAQVGDLTFNDVEELLNQYQHLVFKYVSLSKGLSLRSS-----SPSSSNSEMHSH 411
           PY+FA VGDL   DVE+LLN Y+ LVFKYV LSKGL   +S     SP  ++    +H
Sbjct: 361 PYMFASVGDLKIGDVEDLLNNYKQLVFKYVCLSKGLGDATSLTPCISPLQASKVSENH 398

BLAST of Cp4.1LG01g20050 vs. TAIR 10
Match: AT3G19770.2 (Vacuolar sorting protein 9 (VPS9) domain )

HSP 1 Score: 283.5 bits (724), Expect = 3.2e-76
Identity = 174/307 (56.68%), Postives = 214/307 (69.71%), Query Frame = 0

Query: 137 LLAQKELLKINMYKAPRDKLVCLLGCCKVISNLLLNASIASNENPPGADEFLPVLIYVII 196
           + AQKEL KINMYKAPRDKLVC+L CCKVI+NLLLNASIASNEN PGADEFLPVLIYV I
Sbjct: 1   MAAQKELQKINMYKAPRDKLVCILNCCKVINNLLLNASIASNENAPGADEFLPVLIYVTI 60

Query: 197 KANPPQLHSNLLYIQRYRGQSRLTGEAAYFFTNILSAESFISNIDANALSMEETEFEKNM 256
           KANPPQLHSNLLYIQRYR +S+L GEAAYFFTNILSAESFISNIDA ++S++E EFEKNM
Sbjct: 61  KANPPQLHSNLLYIQRYRRESKLVGEAAYFFTNILSAESFISNIDAKSISLDEAEFEKNM 120

Query: 257 ESARALLSGLSTDVESSSNKSNLDERVARNSELVETSSQASNSGTEPAV-RPNSPESKSS 316
           ESARA +SGL +    + + S    R     E     +Q+ N   E  + +  S +S S 
Sbjct: 121 ESARARISGLDSQTYQTGHGSAPPPR----DESTLQKTQSLNPKRENTLFQSKSSDSLSG 180

Query: 317 T-EGPHVKYQSTVMEVSTLSDLENKGAAMLLNDHTRKQAFRDYPYLFAQVGDLTFNDVEE 376
           T E  ++  ++ + +  ++SDLENKGA  LL D    + F++YPY+FA  GDL   DVE 
Sbjct: 181 TNELLNINSETPMKKAESISDLENKGAT-LLKDTEPSKVFQEYPYIFASAGDLRIGDVEG 240

Query: 377 LLNQYQHLVFKYVSLSKGLSLRSS-SPSSSNSEMHSHHNPESFKEPEDVRDVTSNDESAG 436
           LLN Y+ LVFKYV L+KGL   +S +PSSS  +  S  N  + KE ED R  +S+ +   
Sbjct: 241 LLNSYKQLVFKYVCLTKGLGDGTSLAPSSSPLQASSGFN--TSKESEDHRRSSSDVQMTK 300

Query: 437 ETHTMID 441
           ET   +D
Sbjct: 301 ETDRSVD 300