Cp4.1LG01g15380 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG01g15380
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionXyloglucan endotransglucosylase/hydrolase
LocationCp4.1LG01: 9118279 .. 9120422 (-)
RNA-Seq ExpressionCp4.1LG01g15380
SyntenyCp4.1LG01g15380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTGCCCCTCTTAAGCTTCTTCATTTTGTGCATGTTTTTTGCAGCCCCTTCCTGTGGTACATACTACACGCCTCCAAGCGTCCCACGTTTAACGGATCTCGTTCCCAATGTCTCAATCGATCAATGCTTCGCCAAGATTTTTGGGGCCGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTCAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGGTGCTGGTTTGGTCTCGAGGAGCAAATATCATTATGGGTTCTTCAGTGCTTCAATTAAGCTTCCATCAGGCTTCACTTCAGGGGTTGTAGTGGCTTTTTATGTAAGATTTTCCCCCATTTCTTATTATCCTCTGTTTTGCTTACCAGTTTGGTCAACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTAAGTGCATGAAAGGCAGGATCACTTTTGTTGGTCGTTGTATTTCTCTGTTCTTCTAAAAAGTGCATGGAAATTCAAGCAGGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTTGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGTACCCAGTGGACTATAAGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCAACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGGAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATGTTTTACTCCTATTGTGAAGATACATCCAGATACAAAGTTTTGCCACCAGAGTGCAAATAGATGTATTTTTACCGAGTTTTTTTCCAAGTTTTTGTGAGATCCTACCTCAAGAAAAGAAAAGAAAACTTTCTTTATTTTATAAGAGTGTTAAAACATCTCCATAACAGACACGTTTTAAAAACCGTAAAGGATGACAATATCTGCTAACGGTAGACTTAGGCTGTTACTGAGTATTGTAGTAAAGACATAGGAAGAAGATAGATATTGTAGTAAAGTGTATCCGACAAAGACGAGTATTGGAGTAAAGACATAGGAAGAAGATAGATATTGTAGTAAAGTGTATCCGACAAAGATACGAGCGATGACTGAACAGAGGAGACTCCGATAGGAGATCTTCCATCAGTACCATCCAAAAAGAGGGATTCTCCATCAAGGATTTTGTAAATGCTTGCATTATATGAACTATAAAGAAGTTAAACTAATCTGGCAACTAACGGATTGGTCGGGTCAAAGAAAATTGAAAGATATTAAATTACATAGGGCAAGAAAGCTTCACCCATGGATGCAACCTTTTTGAACTACCCAAATCAGTGATATATGATAGCCAATCCTGAATGAAATGAGAGATACATCAAGAAAAGGGCATAGCCGCATATCATAGATGCCAAATCAAGCCCACAGTGGTACTAATCTTCCTCTCATGGGAGTACAGAGTATGGCATAGGTTTCTATGATTTTCAACTAGAGGAAATCAGACAGGAATTACTACTACCTATTATTTGATGGCACTAAATAAGAGACTGACCAAGACAAATCCACCAGTTATAGTGTATGGCATCCCATATAATATTAACGCCGGCCAAAAGGATAATCACTAATCAGTCAACTAAAGCCAAAAAAGATCAAGCAAGCAGGAATAAGAAACATTCTGAGTTATAAAAACATAAACTCCTGTGGCTTTCTGTTAGTACTGAATTCCAAAGCCTAATCGTAAGCCATATCCATGTATGATTACAGCATGGGCACGAAACATAAAGCTTGA

mRNA sequence

ATGGAATTGCCCCTCTTAAGCTTCTTCATTTTGTGCATGTTTTTTGCAGCCCCTTCCTGTGGTACATACTACACGCCTCCAAGCGTCCCACGTTTAACGGATCTCGTTCCCAATGTCTCAATCGATCAATGCTTCGCCAAGATTTTTGGGGCCGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTCAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGTTTGGTCAACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTTGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGTACCCAGTGGACTATAAGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCAACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGGAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATCATGGGCACGAAACATAAAGCTTGA

Coding sequence (CDS)

ATGGAATTGCCCCTCTTAAGCTTCTTCATTTTGTGCATGTTTTTTGCAGCCCCTTCCTGTGGTACATACTACACGCCTCCAAGCGTCCCACGTTTAACGGATCTCGTTCCCAATGTCTCAATCGATCAATGCTTCGCCAAGATTTTTGGGGCCGCAAATATCCAGCTGATGAGCAATGGGTCCTCTCTCAATCTCACTCTCAACAAACATTCTGGTAAATCATCATTGGATCTTAGCTTCTCCTTTCAGCCCGACTCTGTTTTGATTCATAACTTCTTCTCTTTTCATTTCTGGAGTTTGGTCAACAACCAACGAGATGATGAGGGCCATGTTCAGCTGTCGAATGCAGATGTTTATACCCATTCTCATGACGAGATTGACATAGAGCTGCTGGGACATGACAAGAGAAAAGATTGGGTTATTCAAACAAATATGTATGCAAATGGAAGCGTCAAGACAGGGAGAGAAGAGAAATTCTACCTCTGGTTTGATCCCAGCGTGAAGTACCACGATTACACCATCATTTGGAACAAATATCATACAGTGTTTTTGGTGGATAATGTTCCAATCAGAGAGCTGAGAAATTCTGAAGCTTTCTATCCATCAAAACCCATGTCAGTTTTTGTCACGATATGGGATGGTTCAGAATGGGCAACTCATGGAGGAAAGTACCCAGTGGACTATAAGTATGCACCATATGTGGCTTCTTTTGAAGAAATGGAGATCAATGGAAGTGTATCACCACCAATAGCAACTGTTCCTTCAAGCTCCAAAACCAATGTCTCGAGCCCGGATACCGTGGAGGGTCCAGAATTTATAAAGCTATCACAGGAGCAAATAGATGCCATGGATTGGGCAAGGAGGAAGCTCATCATGGGCACGAAACATAAAGCTTGA

Protein sequence

MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLIMGTKHKA
Homology
BLAST of Cp4.1LG01g15380 vs. ExPASy Swiss-Prot
Match: Q8LC45 (Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis thaliana OX=3702 GN=XTH33 PE=1 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 1.9e-71
Identity = 146/290 (50.34%), Postives = 188/290 (64.83%), Query Frame = 0

Query: 9   FILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLN 68
           F LC      S    +T P+V RLTD    ++I+  F++ FGA NIQ+  NGS   LTL+
Sbjct: 11  FCLCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLD 70

Query: 69  KHSGKSSLDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDI 128
           K SG   +         +   + FFS                  LSNA+ Y  SHDEIDI
Sbjct: 71  KSSGAGLVS-------KNKYHYGFFSARLKLPAGFASGVVVAFYLSNAETYPKSHDEIDI 130

Query: 129 ELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNV 188
           ELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT+IWN +HTVFLVDN+
Sbjct: 131 ELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNI 190

Query: 189 PIRELRNSEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEING- 248
           P+R+  N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV+YKYAP+V S  ++E++G 
Sbjct: 191 PVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGC 250

Query: 249 SVSPPIATVP---SSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 292
           SV+   +T     + S  ++SS D V+G +F  LS+ QI+AMDWARRKL+
Sbjct: 251 SVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINAMDWARRKLM 291

BLAST of Cp4.1LG01g15380 vs. ExPASy Swiss-Prot
Match: Q8LDS2 (Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis thaliana OX=3702 GN=XTH27 PE=2 SV=2)

HSP 1 Score: 197.2 bits (500), Expect = 2.6e-49
Identity = 98/259 (37.84%), Postives = 152/259 (58.69%), Query Frame = 0

Query: 36  VPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P  S ++ + ++FG  N+ +  +G S+ LTL++ +G         F  +   +H FFS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGS-------GFVSNDYYLHGFFSA 85

Query: 96  HFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGR 155
                 +          +SN D+Y  +HDEID E LG+ + K+W +QTN+Y NGS  +GR
Sbjct: 86  SIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHSGR 145

Query: 156 EEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSE---AFYPSKPMSVFVTIW 215
           EE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ +      +PSKPMS++ TIW
Sbjct: 146 EERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTTIW 205

Query: 216 DGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF 275
           DGS+WAT+GGKY V+YKYAPY+A F ++ ++G    PI   P   +         +    
Sbjct: 206 DGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFPRCDEGAAEDMRAAQ---- 265

Query: 276 IKLSQEQIDAMDWARRKLI 292
            +++  Q   MD  RR+L+
Sbjct: 266 -EITPSQRSKMDVFRRRLM 272

BLAST of Cp4.1LG01g15380 vs. ExPASy Swiss-Prot
Match: Q38909 (Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis thaliana OX=3702 GN=XTH28 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 9.9e-49
Identity = 95/227 (41.85%), Postives = 142/227 (62.56%), Query Frame = 0

Query: 36  VPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P +  D+ + ++FG  N+ +  +G S+ LTL++ +G         F  + + +H FFS 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGS-------GFVSNDIYLHGFFS- 85

Query: 96  HFWSLVNNQRDDEGHV----QLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSV 155
              S +    D    V     LSN D+Y  +HDEID E LG+ + ++W IQTN+Y NGS 
Sbjct: 86  ---SSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRIQTNIYGNGST 145

Query: 156 KTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVF 215
             GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ + +    +P+KPMS++
Sbjct: 146 HLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGGDFPAKPMSLY 205

Query: 216 VTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPS 256
            TIWDGS+WAT GGKY V+YKYAPYV+ F ++ ++G    P    PS
Sbjct: 206 STIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPS 241

BLAST of Cp4.1LG01g15380 vs. ExPASy Swiss-Prot
Match: Q38908 (Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis thaliana OX=3702 GN=XTH30 PE=2 SV=2)

HSP 1 Score: 185.3 bits (469), Expect = 1.0e-45
Identity = 97/260 (37.31%), Postives = 152/260 (58.46%), Query Frame = 0

Query: 39  VSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +S ++  + +FG AN+    +  S+ L L++++G         F   ++  H F+S    
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGS-------GFISSNMYQHGFYS---- 89

Query: 99  SLVNNQRDDEGHVQL----SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTG 158
           S++    D    V +    SN DV+  +HDE+DIE LG+ K K W  QTN+Y NGS   G
Sbjct: 90  SMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHRG 149

Query: 159 REEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTI 218
           REE++ LWFDPS ++H Y+I+W  +  +F VD+VPIRE+  ++A    YP+KPM+++ TI
Sbjct: 150 REERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATI 209

Query: 219 WDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPE 278
           WD S+WAT GGKY  +YK+AP+VA F+   ++G    PI  VP     +V   D +E  +
Sbjct: 210 WDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSV---DFLESQD 269

Query: 279 FIKLSQEQIDAMDWARRKLI 292
           +  ++  Q  AM   R++ +
Sbjct: 270 YSSINSHQRAAMRRFRQRFM 275

BLAST of Cp4.1LG01g15380 vs. ExPASy Swiss-Prot
Match: Q8L7H3 (Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis thaliana OX=3702 GN=XTH29 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.5e-41
Identity = 86/213 (40.38%), Postives = 125/213 (58.69%), Query Frame = 0

Query: 39  VSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +  D+  + +FG  N+    +  S+ L L+K++G         F   S+  H FFS    
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGS-------GFISSSMYQHGFFS---- 97

Query: 99  SLVNNQRDDEGHVQL----SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTG 158
           SL+         + +    SN DV+   HDE+DIE LG+ + K W  QTNMY NGS   G
Sbjct: 98  SLIKLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRG 157

Query: 159 REEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTI 218
           REE++ LWFDPS ++H Y+I+W     +F VD+VPIRE+   E     YP KPMS++ TI
Sbjct: 158 REERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATI 217

Query: 219 WDGSEWATHGGKYPVDYKYAPYVASFEEMEING 245
           WD S WAT GGK+ VDY ++P+V+ F+++ ++G
Sbjct: 218 WDASSWATSGGKFGVDYTFSPFVSEFKDIALDG 239

BLAST of Cp4.1LG01g15380 vs. NCBI nr
Match: KAG7032271.1 (putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 572 bits (1474), Expect = 6.76e-205
Identity = 283/306 (92.48%), Postives = 289/306 (94.44%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSFF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWS---------------LVNNQR 120
           SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW                LV+NQR
Sbjct: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWRCWFGLEEQISLWVLHLVHNQR 120

Query: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180
           DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP
Sbjct: 121 DDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDP 180

Query: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYP 240
           SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGK+P
Sbjct: 181 SVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKHP 240

Query: 241 VDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDW 291
           VDY+YAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQ+QIDAMDW
Sbjct: 241 VDYRYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDW 300

BLAST of Cp4.1LG01g15380 vs. NCBI nr
Match: KAG6601490.1 (putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 528 bits (1360), Expect = 6.12e-188
Identity = 259/276 (93.84%), Postives = 266/276 (96.38%), Query Frame = 0

Query: 16  AAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSS 75
           +APSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFGAANIQLMSNGSSLNLTLNKHSGKSS
Sbjct: 14  SAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNGSSLNLTLNKHSGKSS 73

Query: 76  LDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDK 135
           LDLSFSFQPDSVLIHNFFSFHFWSLV+NQRDDEGHVQLSNADVYTHSHDEIDIELLGHDK
Sbjct: 74  LDLSFSFQPDSVLIHNFFSFHFWSLVHNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDK 133

Query: 136 RKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRN 195
           RKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTV         ELRN
Sbjct: 134 RKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTV---------ELRN 193

Query: 196 SEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPS 255
           SEAFYPSKPMSVFVTIWDGSEWATHGG++PVDY+YAPYVASFEEMEINGSVSPPIATVPS
Sbjct: 194 SEAFYPSKPMSVFVTIWDGSEWATHGGRHPVDYRYAPYVASFEEMEINGSVSPPIATVPS 253

Query: 256 SSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           SSKTNVSSPDTVEGPEFIKLSQ+QIDAMDWARRKL+
Sbjct: 254 SSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLM 280

BLAST of Cp4.1LG01g15380 vs. NCBI nr
Match: XP_023512499.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 500 bits (1287), Expect = 9.36e-177
Identity = 256/297 (86.20%), Postives = 263/297 (88.55%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---QLS 120
           SSLNLTLNKHSG   +  S   + F   S+ + + F+              G V    LS
Sbjct: 61  SSLNLTLNKHSGAGLVSRSKYHYGFFSASIKLPSGFT-------------SGVVVAFYLS 120

Query: 121 NADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180
           NADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI
Sbjct: 121 NADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180

Query: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYV 240
           IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYV
Sbjct: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYV 240

Query: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKL+
Sbjct: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLM 284

BLAST of Cp4.1LG01g15380 vs. NCBI nr
Match: XP_022957166.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschata])

HSP 1 Score: 490 bits (1262), Expect = 6.01e-173
Identity = 250/297 (84.18%), Postives = 261/297 (87.88%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSFF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---QLS 120
           SSLNLTLNKHSG   +  S   + F   S+ + + F+              G V    LS
Sbjct: 61  SSLNLTLNKHSGAGLVSRSKYHYGFFSASIKLPSGFT-------------SGVVVAFYLS 120

Query: 121 NADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180
           NADVYTHSHDEIDIELLGHD RKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI
Sbjct: 121 NADVYTHSHDEIDIELLGHDNRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180

Query: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYV 240
           IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDY+YAPYV
Sbjct: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYRYAPYV 240

Query: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF+KLSQ+QIDAMDWARRKL+
Sbjct: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFVKLSQQQIDAMDWARRKLM 284

BLAST of Cp4.1LG01g15380 vs. NCBI nr
Match: XP_022985837.1 (probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Cucurbita maxima])

HSP 1 Score: 481 bits (1239), Expect = 2.06e-169
Identity = 249/299 (83.28%), Postives = 260/299 (86.96%), Query Frame = 0

Query: 1   MELPLLS--FFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMS 60
           MELPLLS  FF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFG ANIQLMS
Sbjct: 1   MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 60

Query: 61  NGSSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---Q 120
           NGSSLNLTLNK SG   +  +   + F   S+ + + F+              G V    
Sbjct: 61  NGSSLNLTLNKLSGAGLVSRNKYHYGFFSASIKLPSGFT-------------SGVVVAFY 120

Query: 121 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 180
           LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY
Sbjct: 121 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 180

Query: 181 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 240
           TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP
Sbjct: 181 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 240

Query: 241 YVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           YVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQ+QIDAMDWARRKL+
Sbjct: 241 YVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLM 286

BLAST of Cp4.1LG01g15380 vs. ExPASy TrEMBL
Match: A0A6J1GYH0 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC111458636 PE=3 SV=1)

HSP 1 Score: 490 bits (1262), Expect = 2.91e-173
Identity = 250/297 (84.18%), Postives = 261/297 (87.88%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSFF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFGAANIQLMSNG
Sbjct: 1   MELPLLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGAANIQLMSNG 60

Query: 61  SSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---QLS 120
           SSLNLTLNKHSG   +  S   + F   S+ + + F+              G V    LS
Sbjct: 61  SSLNLTLNKHSGAGLVSRSKYHYGFFSASIKLPSGFT-------------SGVVVAFYLS 120

Query: 121 NADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180
           NADVYTHSHDEIDIELLGHD RKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI
Sbjct: 121 NADVYTHSHDEIDIELLGHDNRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTI 180

Query: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYV 240
           IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDY+YAPYV
Sbjct: 181 IWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYRYAPYV 240

Query: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF+KLSQ+QIDAMDWARRKL+
Sbjct: 241 ASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFVKLSQQQIDAMDWARRKLM 284

BLAST of Cp4.1LG01g15380 vs. ExPASy TrEMBL
Match: A0A6J1J604 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111483755 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 9.96e-170
Identity = 249/299 (83.28%), Postives = 260/299 (86.96%), Query Frame = 0

Query: 1   MELPLLS--FFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMS 60
           MELPLLS  FF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFG ANIQLMS
Sbjct: 1   MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 60

Query: 61  NGSSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---Q 120
           NGSSLNLTLNK SG   +  +   + F   S+ + + F+              G V    
Sbjct: 61  NGSSLNLTLNKLSGAGLVSRNKYHYGFFSASIKLPSGFT-------------SGVVVAFY 120

Query: 121 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 180
           LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY
Sbjct: 121 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 180

Query: 181 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 240
           TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP
Sbjct: 181 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 240

Query: 241 YVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           YVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQ+QIDAMDWARRKL+
Sbjct: 241 YVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLM 286

BLAST of Cp4.1LG01g15380 vs. ExPASy TrEMBL
Match: A0A6J1JER7 (Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC111483755 PE=3 SV=1)

HSP 1 Score: 481 bits (1239), Expect = 2.13e-169
Identity = 249/299 (83.28%), Postives = 260/299 (86.96%), Query Frame = 0

Query: 1   MELPLLS--FFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMS 60
           MELPLLS  FF+LCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQ FAKIFG ANIQLMS
Sbjct: 22  MELPLLSLSFFLLCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQYFAKIFGGANIQLMS 81

Query: 61  NGSSLNLTLNKHSGKSSLDLS---FSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHV---Q 120
           NGSSLNLTLNK SG   +  +   + F   S+ + + F+              G V    
Sbjct: 82  NGSSLNLTLNKLSGAGLVSRNKYHYGFFSASIKLPSGFT-------------SGVVVAFY 141

Query: 121 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 180
           LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY
Sbjct: 142 LSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDY 201

Query: 181 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 240
           TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP
Sbjct: 202 TIIWNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAP 261

Query: 241 YVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           YVASFEEMEINGS+SPPIATVPSSSKTNVSSPDTVEGPEFIKLSQ+QIDAMDWARRKL+
Sbjct: 262 YVASFEEMEINGSISPPIATVPSSSKTNVSSPDTVEGPEFIKLSQQQIDAMDWARRKLM 307

BLAST of Cp4.1LG01g15380 vs. ExPASy TrEMBL
Match: N0DX87 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE=3 SV=1)

HSP 1 Score: 439 bits (1130), Expect = 3.64e-153
Identity = 222/296 (75.00%), Postives = 240/296 (81.08%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSF  LCM F APSC  YYTPPSVPRLTDLVP VS DQCFAKIFGA+NIQL +NG
Sbjct: 1   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 60

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDD--EGHV---QLSN 120
           SS++LTL+K SG              ++  N + + F+S           G V    LSN
Sbjct: 61  SSVDLTLDKVSGA------------GLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSN 120

Query: 121 ADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTII 180
           ADVY HSHDEIDIELLGHDKRKDWVIQTN+YANGSVKTGREEKFYLWFDPS+KYHDYTII
Sbjct: 121 ADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTII 180

Query: 181 WNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVA 240
           WN YHTVFLVDNVP+RELRNSE FYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APY A
Sbjct: 181 WNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTA 240

Query: 241 SFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           SFEEMEING +  P ATVPSSSK NVS PDT EGPEFIKLSQ+Q+DAMDWARRKL+
Sbjct: 241 SFEEMEINGGILTPTATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDWARRKLM 284

BLAST of Cp4.1LG01g15380 vs. ExPASy TrEMBL
Match: A0A0A0KQA3 (Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G602120 PE=3 SV=1)

HSP 1 Score: 439 bits (1128), Expect = 9.42e-153
Identity = 222/296 (75.00%), Postives = 240/296 (81.08%), Query Frame = 0

Query: 1   MELPLLSFFILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNG 60
           MELPLLSF  LCM F APSC  YYTPPSVPRLTDLVP VS DQCFAKIFGA+NIQL +NG
Sbjct: 8   MELPLLSFIFLCMLFVAPSCSRYYTPPSVPRLTDLVPRVSTDQCFAKIFGASNIQLRNNG 67

Query: 61  SSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDD--EGHV---QLSN 120
           SS++LTL+K SG              ++  N + + F+S           G V    LSN
Sbjct: 68  SSVDLTLDKVSGA------------GLVSRNKYHYGFFSASIKLPSGLTSGVVVAFYLSN 127

Query: 121 ADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTII 180
           ADVY HSHDEIDIELLGHDKRKDWVIQTN+YANGSVKTGREEKFYLWFDPS+KYHDYTII
Sbjct: 128 ADVYPHSHDEIDIELLGHDKRKDWVIQTNIYANGSVKTGREEKFYLWFDPSLKYHDYTII 187

Query: 181 WNKYHTVFLVDNVPIRELRNSEAFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVA 240
           WN YHTVFLVDNVP+RELRNSE FYPSKPMSVFVTIWDGSEWATHGGKYPVDYK+APY A
Sbjct: 188 WNNYHTVFLVDNVPVRELRNSEVFYPSKPMSVFVTIWDGSEWATHGGKYPVDYKHAPYTA 247

Query: 241 SFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 291
           SFEEMEING +  P ATVPSSSK NVS PDT EGPEFIKLSQ+Q+DAMDWARRKL+
Sbjct: 248 SFEEMEINGGILTPKATVPSSSKANVSGPDTAEGPEFIKLSQQQVDAMDWARRKLM 291

BLAST of Cp4.1LG01g15380 vs. TAIR 10
Match: AT1G10550.1 (xyloglucan:xyloglucosyl transferase 33 )

HSP 1 Score: 270.8 bits (691), Expect = 1.3e-72
Identity = 146/290 (50.34%), Postives = 188/290 (64.83%), Query Frame = 0

Query: 9   FILCMFFAAPSCGTYYTPPSVPRLTDLVPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLN 68
           F LC      S    +T P+V RLTD    ++I+  F++ FGA NIQ+  NGS   LTL+
Sbjct: 11  FCLCSLSLVSSHSRKFTTPNVTRLTDQFSKIAIENGFSRRFGAHNIQV--NGSLAKLTLD 70

Query: 69  KHSGKSSLDLSFSFQPDSVLIHNFFSFHFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDI 128
           K SG   +         +   + FFS                  LSNA+ Y  SHDEIDI
Sbjct: 71  KSSGAGLVS-------KNKYHYGFFSARLKLPAGFASGVVVAFYLSNAETYPKSHDEIDI 130

Query: 129 ELLGHDKRKDWVIQTNMYANGSVKTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNV 188
           ELLG  +R DW IQTN+YANGS +TGREEKFY WFDP+  +HDYT+IWN +HTVFLVDN+
Sbjct: 131 ELLGRSRRDDWTIQTNVYANGSTRTGREEKFYFWFDPTQAFHDYTLIWNSHHTVFLVDNI 190

Query: 189 PIRELRNSEAF---YPSKPMSVFVTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEING- 248
           P+R+  N  AF   YPSKPMS++VT+WDGSEWAT GGKYPV+YKYAP+V S  ++E++G 
Sbjct: 191 PVRQFPNRGAFTSAYPSKPMSLYVTVWDGSEWATKGGKYPVNYKYAPFVVSVADVELSGC 250

Query: 249 SVSPPIATVP---SSSKTNVSSPDTVEGPEFIKLSQEQIDAMDWARRKLI 292
           SV+   +T     + S  ++SS D V+G +F  LS+ QI+AMDWARRKL+
Sbjct: 251 SVNNGSSTGSGPCTKSGGSISSLDPVDGQDFATLSKNQINAMDWARRKLM 291

BLAST of Cp4.1LG01g15380 vs. TAIR 10
Match: AT2G01850.1 (endoxyloglucan transferase A3 )

HSP 1 Score: 197.2 bits (500), Expect = 1.9e-50
Identity = 98/259 (37.84%), Postives = 152/259 (58.69%), Query Frame = 0

Query: 36  VPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P  S ++ + ++FG  N+ +  +G S+ LTL++ +G         F  +   +H FFS 
Sbjct: 26  LPITSFEESYTQLFGDKNLFVHQDGKSVRLTLDERTGS-------GFVSNDYYLHGFFSA 85

Query: 96  HFWSLVNNQRDDEGHVQLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTGR 155
                 +          +SN D+Y  +HDEID E LG+ + K+W +QTN+Y NGS  +GR
Sbjct: 86  SIKLPSDYTAGVVVAFYMSNGDMYEKNHDEIDFEFLGNIREKEWRVQTNIYGNGSTHSGR 145

Query: 156 EEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSE---AFYPSKPMSVFVTIW 215
           EE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ +      +PSKPMS++ TIW
Sbjct: 146 EERYNLWFDPTEDFHQYSILWSDSHIIFFVDNVPIREVKRTAEMGGHFPSKPMSLYTTIW 205

Query: 216 DGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPEF 275
           DGS+WAT+GGKY V+YKYAPY+A F ++ ++G    PI   P   +         +    
Sbjct: 206 DGSKWATNGGKYGVNYKYAPYIARFSDLVLHGCPVDPIEQFPRCDEGAAEDMRAAQ---- 265

Query: 276 IKLSQEQIDAMDWARRKLI 292
            +++  Q   MD  RR+L+
Sbjct: 266 -EITPSQRSKMDVFRRRLM 272

BLAST of Cp4.1LG01g15380 vs. TAIR 10
Match: AT1G14720.1 (xyloglucan endotransglucosylase/hydrolase 28 )

HSP 1 Score: 195.3 bits (495), Expect = 7.1e-50
Identity = 95/227 (41.85%), Postives = 142/227 (62.56%), Query Frame = 0

Query: 36  VPNVSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSF 95
           +P +  D+ + ++FG  N+ +  +G S+ LTL++ +G         F  + + +H FFS 
Sbjct: 26  LPLIQFDEGYTQLFGDQNLIVHRDGKSVRLTLDERTGS-------GFVSNDIYLHGFFS- 85

Query: 96  HFWSLVNNQRDDEGHV----QLSNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSV 155
              S +    D    V     LSN D+Y  +HDEID E LG+ + ++W IQTN+Y NGS 
Sbjct: 86  ---SSIKLPADYSAGVVIAFYLSNGDLYEKNHDEIDFEFLGNIRGREWRIQTNIYGNGST 145

Query: 156 KTGREEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVF 215
             GREE++ LWFDP+  +H Y+I+W+  H +F VDNVPIRE++ + +    +P+KPMS++
Sbjct: 146 HLGREERYNLWFDPTEDFHQYSILWSLSHIIFYVDNVPIREVKRTASMGGDFPAKPMSLY 205

Query: 216 VTIWDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPS 256
            TIWDGS+WAT GGKY V+YKYAPYV+ F ++ ++G    P    PS
Sbjct: 206 STIWDGSKWATDGGKYGVNYKYAPYVSQFTDLILHGCAVDPTEKFPS 241

BLAST of Cp4.1LG01g15380 vs. TAIR 10
Match: AT1G32170.1 (xyloglucan endotransglucosylase/hydrolase 30 )

HSP 1 Score: 185.3 bits (469), Expect = 7.3e-47
Identity = 97/260 (37.31%), Postives = 152/260 (58.46%), Query Frame = 0

Query: 39  VSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +S ++  + +FG AN+    +  S+ L L++++G         F   ++  H F+S    
Sbjct: 30  LSFEESLSPLFGDANLVRSPDDLSVRLLLDRYTGS-------GFISSNMYQHGFYS---- 89

Query: 99  SLVNNQRDDEGHVQL----SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTG 158
           S++    D    V +    SN DV+  +HDE+DIE LG+ K K W  QTN+Y NGS   G
Sbjct: 90  SMIKLPADYTAGVVVAFYTSNGDVFEKTHDELDIEFLGNIKGKPWRFQTNLYGNGSTHRG 149

Query: 159 REEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTI 218
           REE++ LWFDPS ++H Y+I+W  +  +F VD+VPIRE+  ++A    YP+KPM+++ TI
Sbjct: 150 REERYRLWFDPSKEFHRYSILWTPHKIIFWVDDVPIREVIRNDAMGADYPAKPMALYATI 209

Query: 219 WDGSEWATHGGKYPVDYKYAPYVASFEEMEINGSVSPPIATVPSSSKTNVSSPDTVEGPE 278
           WD S+WAT GGKY  +YK+AP+VA F+   ++G    PI  VP     +V   D +E  +
Sbjct: 210 WDASDWATSGGKYKANYKFAPFVAEFKSFSLDGCSVDPIQEVPMDCSDSV---DFLESQD 269

Query: 279 FIKLSQEQIDAMDWARRKLI 292
           +  ++  Q  AM   R++ +
Sbjct: 270 YSSINSHQRAAMRRFRQRFM 275

BLAST of Cp4.1LG01g15380 vs. TAIR 10
Match: AT4G18990.1 (xyloglucan endotransglucosylase/hydrolase 29 )

HSP 1 Score: 169.9 bits (429), Expect = 3.2e-42
Identity = 86/213 (40.38%), Postives = 125/213 (58.69%), Query Frame = 0

Query: 39  VSIDQCFAKIFGAANIQLMSNGSSLNLTLNKHSGKSSLDLSFSFQPDSVLIHNFFSFHFW 98
           +  D+  + +FG  N+    +  S+ L L+K++G         F   S+  H FFS    
Sbjct: 38  IFFDEGLSHLFGEGNLIRSPDDRSVRLLLDKYTGS-------GFISSSMYQHGFFS---- 97

Query: 99  SLVNNQRDDEGHVQL----SNADVYTHSHDEIDIELLGHDKRKDWVIQTNMYANGSVKTG 158
           SL+         + +    SN DV+   HDE+DIE LG+ + K W  QTNMY NGS   G
Sbjct: 98  SLIKLPGAYTAGIVVAFYTSNGDVFVKDHDELDIEFLGNLEGKPWRFQTNMYGNGSTNRG 157

Query: 159 REEKFYLWFDPSVKYHDYTIIWNKYHTVFLVDNVPIRELRNSEAF---YPSKPMSVFVTI 218
           REE++ LWFDPS ++H Y+I+W     +F VD+VPIRE+   E     YP KPMS++ TI
Sbjct: 158 REERYRLWFDPSKEFHRYSILWTPTKIIFWVDDVPIREILRKEEMNGDYPQKPMSLYATI 217

Query: 219 WDGSEWATHGGKYPVDYKYAPYVASFEEMEING 245
           WD S WAT GGK+ VDY ++P+V+ F+++ ++G
Sbjct: 218 WDASSWATSGGKFGVDYTFSPFVSEFKDIALDG 239

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LC451.9e-7150.34Probable xyloglucan endotransglucosylase/hydrolase protein 33 OS=Arabidopsis tha... [more]
Q8LDS22.6e-4937.84Probable xyloglucan endotransglucosylase/hydrolase protein 27 OS=Arabidopsis tha... [more]
Q389099.9e-4941.85Probable xyloglucan endotransglucosylase/hydrolase protein 28 OS=Arabidopsis tha... [more]
Q389081.0e-4537.31Probable xyloglucan endotransglucosylase/hydrolase protein 30 OS=Arabidopsis tha... [more]
Q8L7H34.5e-4140.38Probable xyloglucan endotransglucosylase/hydrolase protein 29 OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
KAG7032271.16.76e-20592.48putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbit... [more]
KAG6601490.16.12e-18893.84putative xyloglucan endotransglucosylase/hydrolase protein 33, partial [Cucurbit... [more]
XP_023512499.19.36e-17786.20probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita pepo su... [more]
XP_022957166.16.01e-17384.18probable xyloglucan endotransglucosylase/hydrolase protein 33 [Cucurbita moschat... [more]
XP_022985837.12.06e-16983.28probable xyloglucan endotransglucosylase/hydrolase protein 33 isoform X2 [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1GYH02.91e-17384.18Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J6049.96e-17083.28Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1JER72.13e-16983.28Xyloglucan endotransglucosylase/hydrolase OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
N0DX873.64e-15375.00Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=xth29 PE... [more]
A0A0A0KQA39.42e-15375.00Xyloglucan endotransglucosylase/hydrolase OS=Cucumis sativus OX=3659 GN=Csa_5G60... [more]
Match NameE-valueIdentityDescription
AT1G10550.11.3e-7250.34xyloglucan:xyloglucosyl transferase 33 [more]
AT2G01850.11.9e-5037.84endoxyloglucan transferase A3 [more]
AT1G14720.17.1e-5041.85xyloglucan endotransglucosylase/hydrolase 28 [more]
AT1G32170.17.3e-4737.31xyloglucan endotransglucosylase/hydrolase 30 [more]
AT4G18990.13.2e-4240.38xyloglucan endotransglucosylase/hydrolase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016455Xyloglucan endotransglucosylase/hydrolasePIRSFPIRSF005604EndGlu_transfcoord: 8..294
e-value: 3.0E-67
score: 224.7
IPR000757Glycoside hydrolase family 16PFAMPF00722Glyco_hydro_16coord: 107..229
e-value: 7.4E-33
score: 113.5
IPR000757Glycoside hydrolase family 16PROSITEPS51762GH16_2coord: 22..237
score: 19.842987
NoneNo IPR availableGENE3D2.60.120.200coord: 26..294
e-value: 4.0E-53
score: 182.5
NoneNo IPR availablePANTHERPTHR31062:SF144XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 33-RELATEDcoord: 3..292
IPR044791Beta-glucanase/XTHPANTHERPTHR31062XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATEDcoord: 3..292
IPR013320Concanavalin A-like lectin/glucanase domain superfamilySUPERFAMILY49899Concanavalin A-like lectins/glucanasescoord: 114..292

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG01g15380.1Cp4.1LG01g15380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042546 cell wall biogenesis
biological_process GO:0009831 plant-type cell wall modification involved in multidimensional cell growth
biological_process GO:0010411 xyloglucan metabolic process
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0048046 apoplast
cellular_component GO:0005887 integral component of plasma membrane
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function GO:0016762 xyloglucan:xyloglucosyl transferase activity