Cp4.1LG01g07810 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG01g07810 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 5.9e-96
Identity = 167/305 (54.75%), Postives = 214/305 (70.16%), Query Frame = 0

Query: 54  ECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLF 113
           E  LP++DL  L      ER+AC+  +  A++EWGFFQ+ NHG+  EL+  M +EQ +LF
Sbjct: 47  ERDLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLF 106

Query: 114 GVPFEKKSTSGILDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAAC-YGEFINLREVM 173
            +PFE K  +G+L+ SYRWG PTA      SWSEAFH+PL  +S A C +G+  +LR VM
Sbjct: 107 RLPFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGDLTSLRGVM 166

Query: 174 EELATAMSKLARSLAGVLVECLGHR-------KEIFDDICDESTCFLRLNHYPICPFPGE 233
           +E+A AMS++A ++A  L E L  R          F   CDE+TCFLRLN YP CPF  +
Sbjct: 167 QEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACPFAAD 226

Query: 234 VSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKS 293
             GLVPHTDSDFLT+L QD+ GGL LMK S+WVAV+P P+ALVVNIGDL Q WSN+ YKS
Sbjct: 227 TFGLVPHTDSDFLTVLCQDQVGGLHLMKDSRWVAVRPRPDALVVNIGDLFQAWSNNRYKS 286

Query: 294 VKHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKV 351
           V+H+VV N+  +R S+AYFLCPSY S +G+C +PSPYR FTF EYRK+V++DV   G K+
Sbjct: 287 VEHKVVANAKTDRLSVAYFLCPSYDSLVGTCGEPSPYRAFTFGEYRKKVQEDVRTTGKKI 346

BLAST of Cp4.1LG01g07810 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 336.3 bits (861), Expect = 4.4e-91
Identity = 160/304 (52.63%), Postives = 212/304 (69.74%), Query Frame = 0

Query: 48  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 107
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 108 EQMKLFGVPFEKKSTSGILD-HSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFI 167
           EQ+++F  PF+KKS S      SYRWGTP+A    Q SWSEAFH+P+T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 168 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEV 227
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P EV
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 228 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSV 287
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL Q WSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 288 KHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 347
           +HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 348 LSKF 351
           L +F
Sbjct: 333 LPRF 333

BLAST of Cp4.1LG01g07810 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.5e-78
Identity = 160/346 (46.24%), Postives = 214/346 (61.85%), Query Frame = 0

Query: 18  TDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSV-MEECQLPLIDLTGLESSNEKERRAC 77
           ++SNPPL   Y+ L   + +L +       + ++ ++EC+LP+IDL GL  + +     C
Sbjct: 8   SNSNPPLMSTYKHLFVEQHRLDM------DMGAIDVDECELPVIDLAGLMEAEQ----VC 67

Query: 78  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTS---GILDHSYRWG 137
             ++  A++EWGFFQV NHG+   LL  +   Q+ +F  PF++K T    G    SYRWG
Sbjct: 68  RADMVRAASEWGFFQVTNHGVPQALLRELHDAQVAVFRRPFQEKVTERLLGFSPESYRWG 127

Query: 138 TPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVEC 197
           TPTA    Q SWSEA+HIP+T    +      I  R V+EE++ AM +LA+ LA +L+  
Sbjct: 128 TPTAKCLEQLSWSEAYHIPMTTPRPSTS----IRARAVIEEVSRAMYELAQKLAEILMRG 187

Query: 198 LGHRKEIFDDICD-ESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILH--QDRSGGLQ 257
           L    E    +   E TCFLRLN YP C       GL PHTDSD LTI+H  QD  GGLQ
Sbjct: 188 LPGAGEGETMVTTREETCFLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQ 247

Query: 258 LMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYH 317
           L+KG +WVAVKP+P  L+VN+GDLLQ WSND YKSV+HRV+ N+  ER+S+A+FLCPSYH
Sbjct: 248 LLKGGRWVAVKPSPSTLIVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLCPSYH 307

Query: 318 SPIGSCR-----QPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 352
           + I           + YR FTF EYRKQ+ +DV   G K+GL +FR
Sbjct: 308 TLIIPSSSHVHDDDAHYRSFTFGEYRKQIMEDVRSTGRKIGLHRFR 339

BLAST of Cp4.1LG01g07810 vs. ExPASy Swiss-Prot
Match: Q9C6I4 (Gibberellin 2-beta-dioxygenase 7 OS=Arabidopsis thaliana OX=3702 GN=GA2OX7 PE=1 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.1e-68
Identity = 132/298 (44.30%), Postives = 190/298 (63.76%), Query Frame = 0

Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 116 PFE---KKSTSGILDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVM 175
           PF    ++  S +  +SYRWG P+A  P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 176 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPH 235
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  EV GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 236 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVT 295
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ Q  SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 296 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 351
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

BLAST of Cp4.1LG01g07810 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.3e-39
Identity = 102/293 (34.81%), Postives = 149/293 (50.85%), Query Frame = 0

Query: 57  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVP 116
           +P+IDL GL S            I  A   WGFFQV+NHG++ EL+    +   + F +P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 117 FEKKSTSGILDHSYR-WGTPTAAHPN-QFSWSEAF--HIPLTKVSEAACYGEF-INLREV 176
              K T      +Y  +G+           WS+ +  H+    + +   +  F   +REV
Sbjct: 122 VNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREV 181

Query: 177 MEELATAMSKLARSLAGVLVECLGHRKEIFDDIC--DESTCFLRLNHYPICPFPGEVSGL 236
           ++E    + KL+  +  VL   LG +++ F +    +     LR+N+YP CP P    GL
Sbjct: 182 IDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGL 241

Query: 237 VPHTDSDFLTI-LHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKH 296
            PH+D   +TI L  D+  GLQ+ K   W+ VKP+P A +VNIGD +Q+ SN TYKSV+H
Sbjct: 242 SPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEH 301

Query: 297 RVVTNSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAEYRKQVR 336
           RV+ NS +ER S+A+F  P    PI       S   P  Y   TF +YR  +R
Sbjct: 302 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIR 354

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: XP_022942053.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita moschata])

HSP 1 Score: 721 bits (1862), Expect = 6.93e-262
Identity = 349/358 (97.49%), Postives = 354/358 (98.88%), Query Frame = 0

Query: 6   QKPTRNIENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 65
           +KPTRNIENTTMTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL
Sbjct: 30  RKPTRNIENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 89

Query: 66  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 125
           ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI
Sbjct: 90  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 149

Query: 126 LDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 185
           LDHSYRWGTPTA HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS
Sbjct: 150 LDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 209

Query: 186 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDR 245
           LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDR
Sbjct: 210 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDR 269

Query: 246 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFL 305
           SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQ WSNDTYKSVKH VV NSMRERYSIAYFL
Sbjct: 270 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFL 329

Query: 306 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           CPSYHSPIGSC+QPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFRLVNINTNSNSNN
Sbjct: 330 CPSYHSPIGSCKQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRLVNINTNSNSNN 387

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: XP_022989785.1 (gibberellin 2-beta-dioxygenase 8-like, partial [Cucurbita maxima])

HSP 1 Score: 716 bits (1848), Expect = 9.43e-260
Identity = 347/358 (96.93%), Postives = 351/358 (98.04%), Query Frame = 0

Query: 6   QKPTRNIENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 65
           +KPT NIENTTMTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVM ECQLPLIDLTGL
Sbjct: 30  RKPTGNIENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMGECQLPLIDLTGL 89

Query: 66  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 125
           ESSNEKERRACSREIFGAS EWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI
Sbjct: 90  ESSNEKERRACSREIFGASTEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 149

Query: 126 LDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 185
           LDHSYRWGTPTA HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS
Sbjct: 150 LDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 209

Query: 186 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDR 245
           LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDR
Sbjct: 210 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDR 269

Query: 246 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFL 305
           SGGLQLMKGSQWVAV PNPEALVVNIGDLLQ WSNDTYKSVKHRVV NSMRERYSIAYFL
Sbjct: 270 SGGLQLMKGSQWVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFL 329

Query: 306 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFRLVNINTNSNSNN
Sbjct: 330 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRLVNINTNSNSNN 387

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: XP_023546537.1 (gibberellin 2-beta-dioxygenase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712 bits (1839), Expect = 4.93e-259
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 17  MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 76
           MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 77  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 136
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 137 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 196
           AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 197 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 256
           RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 257 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 316
           WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 300

Query: 317 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 347

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: KAG6600809.1 (Gibberellin 2-beta-dioxygenase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 702 bits (1811), Expect = 9.14e-255
Identity = 340/347 (97.98%), Postives = 343/347 (98.85%), Query Frame = 0

Query: 17  MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 76
           MTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 77  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 136
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 137 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 196
           A HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 ATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 197 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 256
           RKEIFDDIC ESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICHESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 257 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 316
           WVAVKPNPEALVVNIGDLLQ WSNDTYKSVKHRVV NSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300

Query: 317 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           RQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFRLVNINTNSNSNN
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRLVNINTNSNSNN 347

BLAST of Cp4.1LG01g07810 vs. NCBI nr
Match: KAG7031445.1 (Gibberellin 2-beta-dioxygenase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678 bits (1750), Expect = 1.16e-245
Identity = 328/335 (97.91%), Postives = 330/335 (98.51%), Query Frame = 0

Query: 17  MTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 76
           MTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC
Sbjct: 1   MTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKERRAC 60

Query: 77  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRWGTPT 136
           SREIFGASAEWGFFQVINHGIRTELLSRMSKEQ KLFGVPFEKKSTSGILDHSYRWGTPT
Sbjct: 61  SREIFGASAEWGFFQVINHGIRTELLSRMSKEQTKLFGVPFEKKSTSGILDHSYRWGTPT 120

Query: 137 AAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 196
           A HPNQFSWSEAFHI LTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH
Sbjct: 121 ATHPNQFSWSEAFHISLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVECLGH 180

Query: 197 RKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 256
           RKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ
Sbjct: 181 RKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQ 240

Query: 257 WVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIGSC 316
           WVAVKPNPEALVVNIGDLLQ WSNDTYKSVKHRVV NSMRERYSIAYFLCPSYHSPIGSC
Sbjct: 241 WVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFLCPSYHSPIGSC 300

Query: 317 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 351
           RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR
Sbjct: 301 RQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFR 335

BLAST of Cp4.1LG01g07810 vs. ExPASy TrEMBL
Match: A0A6J1FTS8 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447241 PE=3 SV=1)

HSP 1 Score: 721 bits (1862), Expect = 3.36e-262
Identity = 349/358 (97.49%), Postives = 354/358 (98.88%), Query Frame = 0

Query: 6   QKPTRNIENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 65
           +KPTRNIENTTMTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL
Sbjct: 30  RKPTRNIENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 89

Query: 66  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 125
           ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI
Sbjct: 90  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 149

Query: 126 LDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 185
           LDHSYRWGTPTA HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS
Sbjct: 150 LDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 209

Query: 186 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDR 245
           LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDR
Sbjct: 210 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDR 269

Query: 246 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFL 305
           SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQ WSNDTYKSVKH VV NSMRERYSIAYFL
Sbjct: 270 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQAWSNDTYKSVKHMVVANSMRERYSIAYFL 329

Query: 306 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           CPSYHSPIGSC+QPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFRLVNINTNSNSNN
Sbjct: 330 CPSYHSPIGSCKQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRLVNINTNSNSNN 387

BLAST of Cp4.1LG01g07810 vs. ExPASy TrEMBL
Match: A0A6J1JNB9 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111486865 PE=3 SV=1)

HSP 1 Score: 716 bits (1848), Expect = 4.57e-260
Identity = 347/358 (96.93%), Postives = 351/358 (98.04%), Query Frame = 0

Query: 6   QKPTRNIENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGL 65
           +KPT NIENTTMTDSNPPLFH+YELLLQSKAQLQLLNNGNQQINSVM ECQLPLIDLTGL
Sbjct: 30  RKPTGNIENTTMTDSNPPLFHQYELLLQSKAQLQLLNNGNQQINSVMGECQLPLIDLTGL 89

Query: 66  ESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 125
           ESSNEKERRACSREIFGAS EWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI
Sbjct: 90  ESSNEKERRACSREIFGASTEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGI 149

Query: 126 LDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 185
           LDHSYRWGTPTA HPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS
Sbjct: 150 LDHSYRWGTPTATHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARS 209

Query: 186 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDR 245
           LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPG+VSGLVPHTDSDFLTILHQDR
Sbjct: 210 LAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGDVSGLVPHTDSDFLTILHQDR 269

Query: 246 SGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFL 305
           SGGLQLMKGSQWVAV PNPEALVVNIGDLLQ WSNDTYKSVKHRVV NSMRERYSIAYFL
Sbjct: 270 SGGLQLMKGSQWVAVNPNPEALVVNIGDLLQAWSNDTYKSVKHRVVANSMRERYSIAYFL 329

Query: 306 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRLVNINTNSNSNN 363
           CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYK+GLSKFRLVNINTNSNSNN
Sbjct: 330 CPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKIGLSKFRLVNINTNSNSNN 387

BLAST of Cp4.1LG01g07810 vs. ExPASy TrEMBL
Match: A0A6J1FVR3 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita moschata OX=3662 GN=LOC111447734 PE=3 SV=1)

HSP 1 Score: 601 bits (1550), Expect = 2.26e-215
Identity = 292/340 (85.88%), Postives = 310/340 (91.18%), Query Frame = 0

Query: 13  ENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKE 72
           E+TTM +SNPPLF +YELLLQS A LQ L+NG Q  +SV+EECQLPL+DL GLES  EKE
Sbjct: 4   EDTTMMNSNPPLFQQYELLLQSTAHLQPLHNGYQPTSSVLEECQLPLVDLKGLESGKEKE 63

Query: 73  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRW 132
           R ACSREIF ASAEWGFFQVINHGIR+ELLSRMSKEQMKLF VPFEKKSTSGILD+SYRW
Sbjct: 64  RMACSREIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRW 123

Query: 133 GTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVE 192
           G  TA  PNQFSWSEAFHIPLTKVSEA CYG+FI+LREVMEELATAMSKLARSLAGVLVE
Sbjct: 124 GAATATQPNQFSWSEAFHIPLTKVSEADCYGDFIHLREVMEELATAMSKLARSLAGVLVE 183

Query: 193 CLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLM 252
            LGHRKE+F+D+CDESTCFLRLNHYPICPFPGEV+GLVPHTDSD+LTILHQD  GGLQLM
Sbjct: 184 SLGHRKEVFEDMCDESTCFLRLNHYPICPFPGEVTGLVPHTDSDYLTILHQDSGGGLQLM 243

Query: 253 KGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSP 312
           KGSQWVAVKP PEALVVNIGDLLQ WSNDTYKSVKHRVVTNS+RERYS AYFLCPS  S 
Sbjct: 244 KGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVTNSVRERYSTAYFLCPSNQSG 303

Query: 313 IGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 352
           IGSCRQPSPYRHFTF EYRKQVRDDVEK GYKVGLS+FRL
Sbjct: 304 IGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKVGLSRFRL 343

BLAST of Cp4.1LG01g07810 vs. ExPASy TrEMBL
Match: A0A0A0KIW0 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G052540 PE=3 SV=1)

HSP 1 Score: 596 bits (1537), Expect = 2.24e-213
Identity = 288/341 (84.46%), Postives = 307/341 (90.03%), Query Frame = 0

Query: 13  ENTTMT-DSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEK 72
           ENTTM  DSNPPLF +YE LLQS A LQ  NNGN   NSVMEECQLPLIDL GL+SS+EK
Sbjct: 4   ENTTMMMDSNPPLFDQYEFLLQSTAHLQPFNNGNLPTNSVMEECQLPLIDLKGLKSSDEK 63

Query: 73  ERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYR 132
           ER AC REIF AS EWGFFQVINHGI TELL+RM+KEQ+KLFGVPFEKK TSGILD+SYR
Sbjct: 64  ERVACRREIFEASEEWGFFQVINHGIHTELLNRMNKEQIKLFGVPFEKKFTSGILDNSYR 123

Query: 133 WGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLV 192
           WGTPTA HPNQFSWSEAFHIPLTKVSEAACYG+FI LREVMEE+A+AMSK+AR LAGVLV
Sbjct: 124 WGTPTATHPNQFSWSEAFHIPLTKVSEAACYGDFIYLREVMEEVASAMSKVARKLAGVLV 183

Query: 193 ECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQL 252
           E +G RKE+ +DICDESTCFLRLNHYPICPF GEVSGLVPHTDSDFLTILHQD  GGLQ+
Sbjct: 184 ESMGQRKELLEDICDESTCFLRLNHYPICPFSGEVSGLVPHTDSDFLTILHQDSGGGLQV 243

Query: 253 MKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHS 312
           MKGSQW+AVKPNP+ALVVNIGDLLQ WSNDTYKS KHRVV N MRERYSIAYFLCPSYHS
Sbjct: 244 MKGSQWLAVKPNPQALVVNIGDLLQAWSNDTYKSAKHRVVANPMRERYSIAYFLCPSYHS 303

Query: 313 PIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 352
            IGSCR+PSPYRHFTFAEYRKQVRDDVEK GYK+GLS FRL
Sbjct: 304 QIGSCREPSPYRHFTFAEYRKQVRDDVEKTGYKIGLSNFRL 344

BLAST of Cp4.1LG01g07810 vs. ExPASy TrEMBL
Match: A0A6J1JB12 (gibberellin 2-beta-dioxygenase 8-like OS=Cucurbita maxima OX=3661 GN=LOC111483364 PE=3 SV=1)

HSP 1 Score: 591 bits (1523), Expect = 2.93e-211
Identity = 287/340 (84.41%), Postives = 305/340 (89.71%), Query Frame = 0

Query: 13  ENTTMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVMEECQLPLIDLTGLESSNEKE 72
           E+TT+ +SNPPLFH+Y LLLQS A LQ L +G Q  +SV+EECQLPL+DL GLES  EKE
Sbjct: 4   EDTTVMNSNPPLFHQYGLLLQSTAHLQPLQSGYQPTSSVLEECQLPLVDLKGLESGKEKE 63

Query: 73  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKSTSGILDHSYRW 132
           R ACS EIF ASAEWGFFQVINHGIR+ELLSRMSKEQMKLF VPFEKKSTSGILD+SYRW
Sbjct: 64  RMACSSEIFEASAEWGFFQVINHGIRSELLSRMSKEQMKLFAVPFEKKSTSGILDNSYRW 123

Query: 133 GTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVMEELATAMSKLARSLAGVLVE 192
           G  TA  PNQFSWSEAFHIPLTKVSEA CYG FI+LREVMEELATAMSKLARSLAGVLVE
Sbjct: 124 GAATATQPNQFSWSEAFHIPLTKVSEADCYGNFIHLREVMEELATAMSKLARSLAGVLVE 183

Query: 193 CLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLM 252
            LGHRKE+F+D+CDESTCFLRLNHYPICPFPG+V+GLVPHTDSD+LTILHQD  GGLQLM
Sbjct: 184 SLGHRKEVFEDMCDESTCFLRLNHYPICPFPGKVTGLVPHTDSDYLTILHQDSGGGLQLM 243

Query: 253 KGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSP 312
           KGSQWVAVKP PEALVVNIGDLLQ WSNDTYKSVKHRVV NS RERYS AYFLCPS HS 
Sbjct: 244 KGSQWVAVKPIPEALVVNIGDLLQAWSNDTYKSVKHRVVANSARERYSTAYFLCPSDHSG 303

Query: 313 IGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKFRL 352
           IGSCRQPSPYRHFTF EYRKQVRDDVEK GYK GLS+FRL
Sbjct: 304 IGSCRQPSPYRHFTFGEYRKQVRDDVEKTGYKEGLSRFRL 343

BLAST of Cp4.1LG01g07810 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 336.3 bits (861), Expect = 3.1e-92
Identity = 160/304 (52.63%), Postives = 212/304 (69.74%), Query Frame = 0

Query: 48  INSVMEECQLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSK 107
           + +V+EE +LP+ID++ L    E+ER  C   I  AS EWGFFQVINHGI  ++L +M +
Sbjct: 33  VTAVVEEVELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQ 92

Query: 108 EQMKLFGVPFEKKSTSGILD-HSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFI 167
           EQ+++F  PF+KKS S      SYRWGTP+A    Q SWSEAFH+P+T +S+     +F 
Sbjct: 93  EQIRVFREPFDKKSKSEKFSAGSYRWGTPSATSIRQLSWSEAFHVPMTDISDNK---DFT 152

Query: 168 NLREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEV 227
            L   ME+ A+    LA  LA VL E  G     F + C  +TC+LR+N YP CP P EV
Sbjct: 153 TLSSTMEKFASESEALAYMLAEVLAEKSGQNSSFFKENCVRNTCYLRMNRYPPCPKPSEV 212

Query: 228 SGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSV 287
            GL+PHTDSDFLTIL+QD+ GGLQL+K ++W+AVKPNP+AL++NIGDL Q WSN  YKSV
Sbjct: 213 YGLMPHTDSDFLTILYQDQVGGLQLIKDNRWIAVKPNPKALIINIGDLFQAWSNGMYKSV 272

Query: 288 KHRVVTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVG 347
           +HRV+TN   ER+S AYF+CPSY + I        YR+F+F E+R+QV++DV+K G+KVG
Sbjct: 273 EHRVMTNPKVERFSTAYFMCPSYDAVIECSSDRPAYRNFSFREFRQQVQEDVKKFGFKVG 332

Query: 348 LSKF 351
           L +F
Sbjct: 333 LPRF 333

BLAST of Cp4.1LG01g07810 vs. TAIR 10
Match: AT1G50960.1 (gibberellin 2-oxidase 7 )

HSP 1 Score: 260.4 bits (664), Expect = 2.2e-69
Identity = 132/298 (44.30%), Postives = 190/298 (63.76%), Query Frame = 0

Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           +LP+IDL+ L S  E +R+ C +++  A+ EWGFFQ++NHGI  ++   M  E+ KLF  
Sbjct: 39  KLPVIDLSHLTSGEEVKRKRCVKQMVAAAKEWGFFQIVNHGIPKDVFEMMLLEEKKLFDQ 98

Query: 116 PFE---KKSTSGILDHSYRWGTPTAAHPNQFSWSEAFHIPLTKVSEAACYGEFINLREVM 175
           PF    ++  S +  +SYRWG P+A  P Q+S SEAFHI L++VS  +   +  NLR ++
Sbjct: 99  PFSVKVRERFSDLSKNSYRWGNPSATSPAQYSVSEAFHIILSEVSRIS--DDRNNLRTIV 158

Query: 176 EELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYPICPFPGEVSGLVPH 235
           E     ++++A+ +  +L + +    E F++I +    FLRLN Y    F  EV GLVPH
Sbjct: 159 ETYVQEIARVAQMICEILGKQVNVSSEYFENIFELENSFLRLNKYHPSVFGSEVFGLVPH 218

Query: 236 TDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKHRVVT 295
           TD+ FLTIL QD+ GGL+L    QW++VKP  EAL VNIGD+ Q  SN  Y+SV+HRV++
Sbjct: 219 TDTSFLTILSQDQIGGLELENNGQWISVKPCLEALTVNIGDMFQALSNGVYQSVRHRVIS 278

Query: 296 NSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDVEKIGYKVGLSKF 351
            +  ER SIA+F+CP   + I     P  YR F+F EY++Q   DV++ G KVGLS+F
Sbjct: 279 PANIERMSIAFFVCPYLETEIDCFGYPKKYRRFSFREYKEQSEHDVKETGDKVGLSRF 334

BLAST of Cp4.1LG01g07810 vs. TAIR 10
Match: AT5G58660.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 2.3e-42
Identity = 119/372 (31.99%), Postives = 184/372 (49.46%), Query Frame = 0

Query: 16  TMTDSNPPLFHRYELLLQSKAQLQLLNNGNQQINSVM---EECQLPLIDLTGLESSNEKE 75
           ++++S PP F R            + N+ N     V+   ++  +P+IDL  L+   E  
Sbjct: 2   SISESYPPAFRR------------VTNDENPPATPVIVQGKDINIPVIDLERLD--KEIL 61

Query: 76  RRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVPFEKKS---TSGILDHS 135
           R AC         EWG F++ NHG+   L SR+ +    L  +PFEKK     +     S
Sbjct: 62  REACK--------EWGIFRLENHGVPLALTSRLQEISESLLSLPFEKKRELFAAVKSPLS 121

Query: 136 YRWGTPT----------AAHPNQFSWSEAFHIPLTKVS-------------EAACYGEFI 195
           Y WGTP            A  +  +  E F++PL+ +S             +A    +  
Sbjct: 122 YFWGTPALNRSGDALKRGAQASNLTMLEGFNVPLSSLSSLSKLPTSTCCDDDAQEEPKLE 181

Query: 196 NLREVMEELATAMSKLARSLAGVLVECL-----GHRKEIFDDICDESTCFLRLNHYPICP 255
           + R +MEE    ++++A SL   + + L     G+R+    +   EST  +R+  YP   
Sbjct: 182 SFRVLMEEYGKHITRIAVSLFEAIAQTLNLELSGNRR---SEYLSESTGLIRVYRYPQSS 241

Query: 256 FPG--EVSGLVPHTDSDFLTILHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWS 315
                E  G+  HTDS  ++IL +D SGGL++MKG +W  VKP    L+VN+GD++Q  S
Sbjct: 242 EEAAREALGMEVHTDSSVISILREDESGGLEIMKGEEWFCVKPVANTLIVNLGDMMQAIS 301

Query: 316 NDTYKSVKHRV-VTNSMRERYSIAYFLCPSYHSPIGSCRQPSPYRHFTFAEYRKQVRDDV 351
           +D YKSV HRV   N   ER+S+ YF+ P     I S    S Y+ FT++++  QV+ DV
Sbjct: 302 DDEYKSVTHRVKKRNRKTERHSVCYFVFPKRDCVIKS----SNYKLFTYSDFEAQVQADV 344

BLAST of Cp4.1LG01g07810 vs. TAIR 10
Match: AT3G19000.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 5.6e-41
Identity = 107/312 (34.29%), Postives = 158/312 (50.64%), Query Frame = 0

Query: 56  QLPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGV 115
           ++P IDL+ LE ++  ++ A ++EI  A   WGFFQVINHG+ + L  R+ K   + F +
Sbjct: 31  EIPTIDLSSLEDTHH-DKTAIAKEIAEACKRWGFFQVINHGLPSALRHRVEKTAAEFFNL 90

Query: 116 PFEKKSTSGILDHSYRWGTPTAAH-PNQFSWSEAFH--------IPLTKVSEAACYGEFI 175
             E+K      D     G     H  N   W E F         +P +   E     +  
Sbjct: 91  TTEEKRKVK-RDEVNPMGYHDEEHTKNVRDWKEIFDFFLQDSTIVPASPEPEDTELRKLT 150

Query: 176 N--------LREVMEELATAMSKLARSLAGVLVECLGHRKEIFDDICDESTCFLRLNHYP 235
           N         REV +E A  + KLA  L  ++   LG   +      +E T FLR NHYP
Sbjct: 151 NQWPQNPSHFREVCQEYAREVEKLAFRLLELVSISLGLPGDRLTGFFNEQTSFLRFNHYP 210

Query: 236 ICPFPGEVSGLVPHTDSDFLTILHQDRSGGLQLMKGS--QWVAVKPNPEALVVNIGDLLQ 295
            CP P    G+  H D   LT+L QD  GGLQ+ + S  QW+ VKP  +AL++N+G+ +Q
Sbjct: 211 PCPNPELALGVGRHKDGGALTVLAQDSVGGLQVSRRSDGQWIPVKPISDALIINMGNCIQ 270

Query: 296 VWSNDTYKSVKHRVVTNSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAE- 342
           VW+ND Y S +HRVV N+ +ER+SI +F  PS+ + I       S   P  Y+ + + + 
Sbjct: 271 VWTNDEYWSAEHRVVVNTSKERFSIPFFFFPSHEANIEPLEELISEENPPCYKKYNWGKF 330

BLAST of Cp4.1LG01g07810 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 9.5e-41
Identity = 102/293 (34.81%), Postives = 149/293 (50.85%), Query Frame = 0

Query: 57  LPLIDLTGLESSNEKERRACSREIFGASAEWGFFQVINHGIRTELLSRMSKEQMKLFGVP 116
           +P+IDL GL S            I  A   WGFFQV+NHG++ EL+    +   + F +P
Sbjct: 62  IPIIDLEGLFSEEGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMP 121

Query: 117 FEKKSTSGILDHSYR-WGTPTAAHPN-QFSWSEAF--HIPLTKVSEAACYGEF-INLREV 176
              K T      +Y  +G+           WS+ +  H+    + +   +  F   +REV
Sbjct: 122 VNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREV 181

Query: 177 MEELATAMSKLARSLAGVLVECLGHRKEIFDDIC--DESTCFLRLNHYPICPFPGEVSGL 236
           ++E    + KL+  +  VL   LG +++ F +    +     LR+N+YP CP P    GL
Sbjct: 182 IDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELALGL 241

Query: 237 VPHTDSDFLTI-LHQDRSGGLQLMKGSQWVAVKPNPEALVVNIGDLLQVWSNDTYKSVKH 296
            PH+D   +TI L  D+  GLQ+ K   W+ VKP+P A +VNIGD +Q+ SN TYKSV+H
Sbjct: 242 SPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEH 301

Query: 297 RVVTNSMRERYSIAYFLCPSYHSPIG------SCRQPSPYRHFTFAEYRKQVR 336
           RV+ NS +ER S+A+F  P    PI       S   P  Y   TF +YR  +R
Sbjct: 302 RVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIR 354