Cp4.1LG00g02590 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG00g02590 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 437.6 bits (1124), Expect = 1.5e-121
Identity = 255/414 (61.59%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 23  HGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN----------------------- 82
           H HI+EV          IV SK+CG+AP CGFSD+KN                       
Sbjct: 8   HSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLCIAVVLCL 67

Query: 83  ---------------------------NVAAFAISLFSLWASGWEATPRQSYGFFRIEIL 142
                                      +VAAFAISLFSLWA+GWEATPRQ+YGFFRIEIL
Sbjct: 68  VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEIL 127

Query: 143 GALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLGHEHGH 202
           GALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+LLGH+HGH
Sbjct: 128 GALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGH 187

Query: 203 AHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHH-HEGG--KGAARD-HHAH 262
           +H HGH HG   HDH           H H HG+++TTHHHHH HE G   G   D HHAH
Sbjct: 188 SHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAH 247

Query: 263 KEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIW 322
            + VT  LL+           +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGGAIIW
Sbjct: 248 GD-VTEQLLD-------KSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGGAIIW 307

Query: 323 YKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVA 382
           Y PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+EVVA
Sbjct: 308 YNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEMEEVVA 367

BLAST of Cp4.1LG00g02590 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 432.6 bits (1111), Expect = 4.8e-120
Identity = 250/421 (59.38%), Postives = 287/421 (68.17%), Query Frame = 0

Query: 14  QKREMEVQDHGHIIEVCGDVQAVGPSIVG-SKICGDAPSCGFSDAK-------------- 73
           +K +  + +H H++++CG+V +   S+VG  K CG+AP CGFSDAK              
Sbjct: 14  KKMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMR 73

Query: 74  ------------------------------------NNVAAFAISLFSLWASGWEATPRQ 133
                                               ++VAAFAISLFSLWASGW+A P+Q
Sbjct: 74  KLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQ 133

Query: 134 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 193
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNI M
Sbjct: 134 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAM 193

Query: 194 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 253
           A+LLGH+HGH H H H +GHG H H HG            HGI+ T HHH          
Sbjct: 194 AILLGHDHGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHH---------- 253

Query: 254 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 313
                H E               S    V+  QKKQRN+N+QGAYLHVLGDSIQS+GVMI
Sbjct: 254 --DSGHDE---------------SQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMI 313

Query: 314 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 373
           GGAIIWYKPE+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE
Sbjct: 314 GGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCE 373

Query: 374 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 384
           ++EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+IDYI+RE+NISHVTIQIER
Sbjct: 374 IEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIER 393

BLAST of Cp4.1LG00g02590 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 4.3e-113
Identity = 235/335 (70.15%), Postives = 264/335 (78.81%), Query Frame = 0

Query: 59  NNVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLIN 118
           ++VAAFAISLFSLWA+GWEATP+QSYGFFRIEILGALVSIQ+IWLLAGILVYEAIVRLIN
Sbjct: 93  SDVAAFAISLFSLWAAGWEATPQQSYGFFRIEILGALVSIQLIWLLAGILVYEAIVRLIN 152

Query: 119 GPGEVKGLLMFAVSAFGLVVNIGMALLLGHEHGHAHSHGHSHGHG-EHDHG-----HGKH 178
             GEV+G LMFAVSAFGL VNI MA+LLGH+HGH H HGH HGH  +HDHG     H  H
Sbjct: 153 ESGEVQGSLMFAVSAFGLFVNIIMAVLLGHDHGHGHGHGHGHGHSHDHDHGGSDHDHHHH 212

Query: 179 EHGGEGHGHH----HGISITTHHHHHHEGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHS 238
           E    GH HH    HG SIT + HHH     G    HH  +EP+     +  C+  +S +
Sbjct: 213 EDQEHGHVHHHEDGHGNSITVNLHHH----PGTGHHHHDAEEPLLKS--DAGCDSTQSGA 272

Query: 239 PKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIV 298
                 +K +RNINV  AYLHVLGDSIQSIGVMIGGAIIWYKPE+KI+DLICTLIFS IV
Sbjct: 273 KDA---KKARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIV 332

Query: 299 LCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKILLACHV 358
           L TTI+MLRNILEVLMESTPREIDAT LE GL +MD VVA+HELHIWAITVGK+LLACHV
Sbjct: 333 LFTTIKMLRNILEVLMESTPREIDATSLENGLRDMDGVVAVHELHIWAITVGKVLLACHV 392

Query: 359 IIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 384
            I  +A+AD +LDKVI YI+ EYNISHVTIQIER+
Sbjct: 393 TITQDADADQMLDKVIGYIKSEYNISHVTIQIERE 418

BLAST of Cp4.1LG00g02590 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-90
Identity = 188/324 (58.02%), Postives = 222/324 (68.52%), Query Frame = 0

Query: 60  NVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLING 119
           +V AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA+ RL+  
Sbjct: 69  DVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRLVQE 128

Query: 120 PG-EVKGLLMFAVSAFGLVVNIGMALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEG 179
              +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH HG   H +G+       
Sbjct: 129 TNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHG---HSYGE------- 188

Query: 180 HGHHHGISITTHHHHHHEGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQ 239
                                                            + ++++  K+ 
Sbjct: 189 ------------------------------------------------RAEQLLEKSKEI 248

Query: 240 RNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRN 299
           RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FS IVL TTI+MLR+
Sbjct: 249 RNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVLGTTIKMLRS 308

Query: 300 ILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADM 359
           ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV ++PEA  +M
Sbjct: 309 ILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKVRPEAGDEM 334

Query: 360 VLDKVIDYIRREYNISHVTIQIER 383
           VL+KVIDYI REY ISHVTIQIER
Sbjct: 369 VLNKVIDYIWREYRISHVTIQIER 334

BLAST of Cp4.1LG00g02590 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.3e-64
Identity = 137/325 (42.15%), Postives = 200/325 (61.54%), Query Frame = 0

Query: 59  NNVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLIN 118
           ++VA   +SL ++  S WEA PR S+GF R+E+L A +S+Q+IWL++G++++EAI RL++
Sbjct: 93  SDVAGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLS 152

Query: 119 GPGEVKGLLMFAVSAFGLVVNIGMALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEG 178
              EV G +MF +SAFG  +N+ M L LGH H H H          HDH           
Sbjct: 153 RSREVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHH----------HDH----------- 212

Query: 179 HGHHHGISITTHHHHHHEGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQ 238
             HHH      HH+H H+         H HKE V         E+   +  K  K   K+
Sbjct: 213 --HHH------HHNHKHQ-------HQHHHKEVVAEE------EEEEMNPLKGEKSSSKE 272

Query: 239 RNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRN 298
            NIN+QGAYLH + D IQS+GVMIGG IIW KP++ ++DLICTL+FSA  L  T+ +L+N
Sbjct: 273 MNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLVFSAFALAATLPILKN 332

Query: 299 ILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADM 358
           I  +LME  PR++D  KLE+GL  +D V  +++LH+W ITVG+I+L+CH++ +P A+   
Sbjct: 333 IFGILMERVPRDMDIEKLERGLKRIDGVKIVYDLHVWEITVGRIVLSCHILPEPGASPKE 375

Query: 359 VLDKVIDYIRREYNISHVTIQIERQ 384
           ++  V ++ R+ Y I H T+Q+E +
Sbjct: 393 IITGVRNFCRKSYGIYHATVQVESE 375

BLAST of Cp4.1LG00g02590 vs. NCBI nr
Match: XP_023520863.1 (metal tolerance protein A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703 bits (1815), Expect = 8.03e-254
Identity = 370/421 (87.89%), Postives = 371/421 (88.12%), Query Frame = 0

Query: 13  MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN------------ 72
           MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+            
Sbjct: 1   MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSNDAKERAASMR 60

Query: 73  --------------------------------------VAAFAISLFSLWASGWEATPRQ 132
                                                 VAAFAISLFSLWASGWEATPRQ
Sbjct: 61  KLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQ 120

Query: 133 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 192
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM
Sbjct: 121 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 180

Query: 193 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 252
           ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA
Sbjct: 181 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 240

Query: 253 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 312
           RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 300

Query: 313 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 372
           GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 373 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 383
           MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER
Sbjct: 361 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 420

BLAST of Cp4.1LG00g02590 vs. NCBI nr
Match: XP_022953632.1 (metal tolerance protein A2-like [Cucurbita moschata])

HSP 1 Score: 677 bits (1746), Expect = 2.50e-243
Identity = 363/421 (86.22%), Postives = 364/421 (86.46%), Query Frame = 0

Query: 13  MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN------------ 72
           MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+            
Sbjct: 1   MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSNDAKERAASMR 60

Query: 73  --------------------------------------VAAFAISLFSLWASGWEATPRQ 132
                                                 VAAFAISLFSLWASGWEATPRQ
Sbjct: 61  KLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQ 120

Query: 133 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 192
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM
Sbjct: 121 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 180

Query: 193 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 252
           ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHH H EG KGAA
Sbjct: 181 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHDHQEG-KGAA 240

Query: 253 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 312
            DHHA  EPVTTPLLEG CEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 SDHHAPTEPVTTPLLEGGCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 300

Query: 313 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 372
           GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 373 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 383
           MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER
Sbjct: 361 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 420

BLAST of Cp4.1LG00g02590 vs. NCBI nr
Match: KAG7013911.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 675 bits (1741), Expect = 1.25e-242
Identity = 359/416 (86.30%), Postives = 360/416 (86.54%), Query Frame = 0

Query: 18  MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN----------------- 77
           MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+                 
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSNDAKERAASMRKLFIA 60

Query: 78  ---------------------------------VAAFAISLFSLWASGWEATPRQSYGFF 137
                                            VAAFAISLFSLWASGWEATPRQSYGFF
Sbjct: 61  VVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFF 120

Query: 138 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 197
           RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG
Sbjct: 121 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 180

Query: 198 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAARDHHA 257
           HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHH HHE GKGAA DHHA
Sbjct: 181 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHDHHEEGKGAASDHHA 240

Query: 258 HKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII 317
             EPVTTPLLEG CEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII
Sbjct: 241 QTEPVTTPLLEGGCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII 300

Query: 318 WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV 377
           WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV
Sbjct: 301 WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV 360

Query: 378 AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 383
           AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ
Sbjct: 361 AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 416

BLAST of Cp4.1LG00g02590 vs. NCBI nr
Match: KAG6575371.1 (Metal tolerance protein A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 672 bits (1733), Expect = 2.06e-241
Identity = 358/416 (86.06%), Postives = 359/416 (86.30%), Query Frame = 0

Query: 18  MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN----------------- 77
           MEVQDHGHIIEVCGDVQAVG SIVGSKICGDAPSCGFSDAKN+                 
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGSSIVGSKICGDAPSCGFSDAKNSSNDAKERAASMRKLFIA 60

Query: 78  ---------------------------------VAAFAISLFSLWASGWEATPRQSYGFF 137
                                            VAAFAISLFSLWASGWEATPRQSYGFF
Sbjct: 61  VVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFF 120

Query: 138 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 197
           RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG
Sbjct: 121 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 180

Query: 198 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAARDHHA 257
           HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHH HHE GKGAA DHHA
Sbjct: 181 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHDHHEEGKGAASDHHA 240

Query: 258 HKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII 317
             EPVTTPLLEG CEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII
Sbjct: 241 QTEPVTTPLLEGGCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAII 300

Query: 318 WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV 377
           WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV
Sbjct: 301 WYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVV 360

Query: 378 AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 383
           AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ
Sbjct: 361 AIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 416

BLAST of Cp4.1LG00g02590 vs. NCBI nr
Match: XP_022992261.1 (metal tolerance protein 1-like [Cucurbita maxima])

HSP 1 Score: 661 bits (1705), Expect = 5.90e-237
Identity = 356/428 (83.18%), Postives = 358/428 (83.64%), Query Frame = 0

Query: 13  MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN------------ 72
           MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDA N+            
Sbjct: 1   MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDANNSSNDAKERAASMR 60

Query: 73  --------------------------------------VAAFAISLFSLWASGWEATPRQ 132
                                                 VAAFAISLFSLWASGWEATPRQ
Sbjct: 61  RLSIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQ 120

Query: 133 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 192
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGP EVKG LMFAVSAFGLVVNIGM
Sbjct: 121 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPSEVKGFLMFAVSAFGLVVNIGM 180

Query: 193 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHG-------GEGHGHHHGISITTHHHHHH 252
           ALLLGHE+GHAHSHGHSHGHG HDHGHGKHEHG       GEGHGHHHGISITTHHHHHH
Sbjct: 181 ALLLGHEYGHAHSHGHSHGHGAHDHGHGKHEHGHGKHEHGGEGHGHHHGISITTHHHHHH 240

Query: 253 EGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI 312
           E GKGAA DHHAHKE VTT LLE CCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI
Sbjct: 241 EEGKGAASDHHAHKESVTTSLLEVCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI 300

Query: 313 QSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATK 372
           QSIGVMIGGAIIWYKPEY ILDLI  LIFSAIVLCTTIQMLRNILEVLMESTPREIDATK
Sbjct: 301 QSIGVMIGGAIIWYKPEYMILDLISALIFSAIVLCTTIQMLRNILEVLMESTPREIDATK 360

Query: 373 LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISH 383
           LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLD VIDYIRREYNISH
Sbjct: 361 LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDNVIDYIRREYNISH 420

BLAST of Cp4.1LG00g02590 vs. ExPASy TrEMBL
Match: A0A6J1GNS5 (metal tolerance protein A2-like OS=Cucurbita moschata OX=3662 GN=LOC111456106 PE=4 SV=1)

HSP 1 Score: 677 bits (1746), Expect = 1.21e-243
Identity = 363/421 (86.22%), Postives = 364/421 (86.46%), Query Frame = 0

Query: 13  MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN------------ 72
           MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+            
Sbjct: 1   MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSNDAKERAASMR 60

Query: 73  --------------------------------------VAAFAISLFSLWASGWEATPRQ 132
                                                 VAAFAISLFSLWASGWEATPRQ
Sbjct: 61  KLFIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQ 120

Query: 133 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 192
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM
Sbjct: 121 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 180

Query: 193 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 252
           ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHH H EG KGAA
Sbjct: 181 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHDHQEG-KGAA 240

Query: 253 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 312
            DHHA  EPVTTPLLEG CEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI
Sbjct: 241 SDHHAPTEPVTTPLLEGGCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 300

Query: 313 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 372
           GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE
Sbjct: 301 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 360

Query: 373 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 383
           MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER
Sbjct: 361 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 420

BLAST of Cp4.1LG00g02590 vs. ExPASy TrEMBL
Match: A0A6J1JT29 (metal tolerance protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488635 PE=4 SV=1)

HSP 1 Score: 661 bits (1705), Expect = 2.86e-237
Identity = 356/428 (83.18%), Postives = 358/428 (83.64%), Query Frame = 0

Query: 13  MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN------------ 72
           MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDA N+            
Sbjct: 1   MQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDANNSSNDAKERAASMR 60

Query: 73  --------------------------------------VAAFAISLFSLWASGWEATPRQ 132
                                                 VAAFAISLFSLWASGWEATPRQ
Sbjct: 61  RLSIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQ 120

Query: 133 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 192
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGP EVKG LMFAVSAFGLVVNIGM
Sbjct: 121 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPSEVKGFLMFAVSAFGLVVNIGM 180

Query: 193 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHG-------GEGHGHHHGISITTHHHHHH 252
           ALLLGHE+GHAHSHGHSHGHG HDHGHGKHEHG       GEGHGHHHGISITTHHHHHH
Sbjct: 181 ALLLGHEYGHAHSHGHSHGHGAHDHGHGKHEHGHGKHEHGGEGHGHHHGISITTHHHHHH 240

Query: 253 EGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI 312
           E GKGAA DHHAHKE VTT LLE CCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI
Sbjct: 241 EEGKGAASDHHAHKESVTTSLLEVCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSI 300

Query: 313 QSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATK 372
           QSIGVMIGGAIIWYKPEY ILDLI  LIFSAIVLCTTIQMLRNILEVLMESTPREIDATK
Sbjct: 301 QSIGVMIGGAIIWYKPEYMILDLISALIFSAIVLCTTIQMLRNILEVLMESTPREIDATK 360

Query: 373 LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISH 383
           LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLD VIDYIRREYNISH
Sbjct: 361 LEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDNVIDYIRREYNISH 420

BLAST of Cp4.1LG00g02590 vs. ExPASy TrEMBL
Match: A0A1S3CB54 (metal tolerance protein 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498976 PE=4 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 1.39e-212
Identity = 330/429 (76.92%), Postives = 340/429 (79.25%), Query Frame = 0

Query: 12  HMQKREMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN----------- 71
           H  + +MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+           
Sbjct: 2   HTTQLQMEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASM 61

Query: 72  ---------------------------------------VAAFAISLFSLWASGWEATPR 131
                                                  VAAFAISLFSLWASGWEATPR
Sbjct: 62  RKLCIAVVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPR 121

Query: 132 QSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIG 191
           QSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVS FGLVVNI 
Sbjct: 122 QSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSTFGLVVNIA 181

Query: 192 MALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGA 251
           MALLLGHEHGHAHSHGH+HGHGEHDHGHG HEHG E H H HGIS+T HHHHH E  KGA
Sbjct: 182 MALLLGHEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHHHDE--KGA 241

Query: 252 ARD-------HHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDS 311
           A D       HH HKE  T PLL+         S KV K QKKQRNINVQGAYLHVLGDS
Sbjct: 242 ASDGVEHHHHHHEHKESTTVPLLDS--------SRKVTKTQKKQRNINVQGAYLHVLGDS 301

Query: 312 IQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDAT 371
           IQSIGVMIGGAIIWY+PEY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DAT
Sbjct: 302 IQSIGVMIGGAIIWYRPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDAT 361

Query: 372 KLEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNIS 383
           KLEKGLCEM+EVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVI+YIRREYNIS
Sbjct: 362 KLEKGLCEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNIS 420

BLAST of Cp4.1LG00g02590 vs. ExPASy TrEMBL
Match: E5GC92 (Metal tolerance protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 8.80e-212
Identity = 329/423 (77.78%), Postives = 337/423 (79.67%), Query Frame = 0

Query: 18  MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN----------------- 77
           MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+                 
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLCIA 60

Query: 78  ---------------------------------VAAFAISLFSLWASGWEATPRQSYGFF 137
                                            VAAFAISLFSLWASGWEATPRQSYGFF
Sbjct: 61  VVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFF 120

Query: 138 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 197
           RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVS FGLVVNI MALLLG
Sbjct: 121 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSTFGLVVNIAMALLLG 180

Query: 198 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAARD--- 257
           HEHGHAHSHGH+HGHGEHDHGHG HEHG E H H HGIS+T HHHHH E  KGAA D   
Sbjct: 181 HEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHHHDE--KGAASDGVE 240

Query: 258 ----HHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGV 317
               HH HKE  T PLL+         S KV K QKKQRNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 HHHHHHEHKESTTVPLLDS--------SRKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 300

Query: 318 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 377
           MIGGAIIWY+PEY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DATKLEKGL
Sbjct: 301 MIGGAIIWYRPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKGL 360

Query: 378 CEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQI 383
           CEM+EVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVI+YIRREYNISHVTIQI
Sbjct: 361 CEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 413

BLAST of Cp4.1LG00g02590 vs. ExPASy TrEMBL
Match: A0A1S3CBM2 (metal tolerance protein 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498976 PE=4 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 8.80e-212
Identity = 329/423 (77.78%), Postives = 337/423 (79.67%), Query Frame = 0

Query: 18  MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNN----------------- 77
           MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN+                 
Sbjct: 1   MEVQDHGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKNSSKDAKERSASMRKLCIA 60

Query: 78  ---------------------------------VAAFAISLFSLWASGWEATPRQSYGFF 137
                                            VAAFAISLFSLWASGWEATPRQSYGFF
Sbjct: 61  VVLCIVFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWEATPRQSYGFF 120

Query: 138 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLG 197
           RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVS FGLVVNI MALLLG
Sbjct: 121 RIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSTFGLVVNIAMALLLG 180

Query: 198 HEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAARD--- 257
           HEHGHAHSHGH+HGHGEHDHGHG HEHG E H H HGIS+T HHHHH E  KGAA D   
Sbjct: 181 HEHGHAHSHGHNHGHGEHDHGHGSHEHGEEDHRHRHGISVTMHHHHHDE--KGAASDGVE 240

Query: 258 ----HHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGV 317
               HH HKE  T PLL+         S KV K QKKQRNINVQGAYLHVLGDSIQSIGV
Sbjct: 241 HHHHHHEHKESTTVPLLDS--------SRKVTKTQKKQRNINVQGAYLHVLGDSIQSIGV 300

Query: 318 MIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGL 377
           MIGGAIIWY+PEY ILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPRE+DATKLEKGL
Sbjct: 301 MIGGAIIWYRPEYMILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREVDATKLEKGL 360

Query: 378 CEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQI 383
           CEM+EVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVI+YIRREYNISHVTIQI
Sbjct: 361 CEMEEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIEYIRREYNISHVTIQI 413

BLAST of Cp4.1LG00g02590 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 437.6 bits (1124), Expect = 1.1e-122
Identity = 255/414 (61.59%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 23  HGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN----------------------- 82
           H HI+EV          IV SK+CG+AP CGFSD+KN                       
Sbjct: 8   HSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLCIAVVLCL 67

Query: 83  ---------------------------NVAAFAISLFSLWASGWEATPRQSYGFFRIEIL 142
                                      +VAAFAISLFSLWA+GWEATPRQ+YGFFRIEIL
Sbjct: 68  VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEIL 127

Query: 143 GALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLGHEHGH 202
           GALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+LLGH+HGH
Sbjct: 128 GALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGH 187

Query: 203 AHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHH-HEGG--KGAARD-HHAH 262
           +H HGH HG   HDH           H H HG+++TTHHHHH HE G   G   D HHAH
Sbjct: 188 SHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAH 247

Query: 263 KEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIW 322
            + VT  LL+           +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGGAIIW
Sbjct: 248 GD-VTEQLLD-------KSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGGAIIW 307

Query: 323 YKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVA 382
           Y PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+EVVA
Sbjct: 308 YNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEMEEVVA 367

BLAST of Cp4.1LG00g02590 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 437.6 bits (1124), Expect = 1.1e-122
Identity = 255/414 (61.59%), Postives = 290/414 (70.05%), Query Frame = 0

Query: 23  HGHIIEVCGDVQAVGPSIVGSKICGDAPSCGFSDAKN----------------------- 82
           H HI+EV          IV SK+CG+AP CGFSD+KN                       
Sbjct: 8   HSHIVEVNVGKSDEERIIVASKVCGEAP-CGFSDSKNASGDAHERSASMRKLCIAVVLCL 67

Query: 83  ---------------------------NVAAFAISLFSLWASGWEATPRQSYGFFRIEIL 142
                                      +VAAFAISLFSLWA+GWEATPRQ+YGFFRIEIL
Sbjct: 68  VFMSVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEIL 127

Query: 143 GALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLGHEHGH 202
           GALVSIQ+IWLL GILVYEAI+R++    EV G LMF V+AFGLVVNI MA+LLGH+HGH
Sbjct: 128 GALVSIQLIWLLTGILVYEAIIRIVTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGH 187

Query: 203 AHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHH-HEGG--KGAARD-HHAH 262
           +H HGH HG   HDH           H H HG+++TTHHHHH HE G   G   D HHAH
Sbjct: 188 SHGHGHGHG---HDH-----------HNHSHGVTVTTHHHHHDHEHGHSHGHGEDKHHAH 247

Query: 263 KEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIW 322
            + VT  LL+           +V   +K++RNIN+QGAYLHVLGDSIQS+GVMIGGAIIW
Sbjct: 248 GD-VTEQLLD-------KSKTQVAAKEKRKRNINLQGAYLHVLGDSIQSVGVMIGGAIIW 307

Query: 323 YKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVA 382
           Y PE+KI+DLICTL FS IVL TTI M+RNILEVLMESTPREIDATKLEKGL EM+EVVA
Sbjct: 308 YNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDATKLEKGLLEMEEVVA 367

BLAST of Cp4.1LG00g02590 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 432.6 bits (1111), Expect = 3.4e-121
Identity = 250/421 (59.38%), Postives = 287/421 (68.17%), Query Frame = 0

Query: 14  QKREMEVQDHGHIIEVCGDVQAVGPSIVG-SKICGDAPSCGFSDAK-------------- 73
           +K +  + +H H++++CG+V +   S+VG  K CG+AP CGFSDAK              
Sbjct: 14  KKMKDHIHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMR 73

Query: 74  ------------------------------------NNVAAFAISLFSLWASGWEATPRQ 133
                                               ++VAAFAISLFSLWASGW+A P+Q
Sbjct: 74  KLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQ 133

Query: 134 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGM 193
           SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNI M
Sbjct: 134 SYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAM 193

Query: 194 ALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAA 253
           A+LLGH+HGH H H H +GHG H H HG            HGI+ T HHH          
Sbjct: 194 AILLGHDHGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHH---------- 253

Query: 254 RDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMI 313
                H E               S    V+  QKKQRN+N+QGAYLHVLGDSIQS+GVMI
Sbjct: 254 --DSGHDE---------------SQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMI 313

Query: 314 GGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCE 373
           GGAIIWYKPE+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE
Sbjct: 314 GGAIIWYKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCE 373

Query: 374 MDEVVAIHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIER 384
           ++EVVA+HELHIWAITVGK+LLACHV I+PEA ADMVLDK+IDYI+RE+NISHVTIQIER
Sbjct: 374 IEEVVAVHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIER 393

BLAST of Cp4.1LG00g02590 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 431.4 bits (1108), Expect = 7.5e-121
Identity = 249/415 (60.00%), Postives = 284/415 (68.43%), Query Frame = 0

Query: 20  VQDHGHIIEVCGDVQAVGPSIVG-SKICGDAPSCGFSDAK-------------------- 79
           + +H H++++CG+V +   S+VG  K CG+AP CGFSDAK                    
Sbjct: 59  IHEHDHMVQICGEVSSGETSLVGIKKTCGEAP-CGFSDAKTSSIEAQERAASMRKLLIAV 118

Query: 80  ------------------------------NNVAAFAISLFSLWASGWEATPRQSYGFFR 139
                                         ++VAAFAISLFSLWASGW+A P+QSYGFFR
Sbjct: 119 LLCAIFIVVEVVGGIKANSLAILTDAAHLLSDVAAFAISLFSLWASGWKANPQQSYGFFR 178

Query: 140 IEILGALVSIQMIWLLAGILVYEAIVRLINGPGEVKGLLMFAVSAFGLVVNIGMALLLGH 199
           IEILGALVSIQMIWLLAGILVYEAIVRL NG GEV+G LMFAVSA GL+VNI MA+LLGH
Sbjct: 179 IEILGALVSIQMIWLLAGILVYEAIVRLNNGSGEVEGSLMFAVSAVGLLVNIAMAILLGH 238

Query: 200 EHGHAHSHGHSHGHGEHDHGHGKHEHGGEGHGHHHGISITTHHHHHHEGGKGAARDHHAH 259
           +HGH H H H +GHG H H HG            HGI+ T HHH               H
Sbjct: 239 DHGHGHGHSHDNGHG-HSHDHG------------HGIAATEHHH------------DSGH 298

Query: 260 KEPVTTPLLEGCCEDGRSHSPKVIKMQKKQRNINVQGAYLHVLGDSIQSIGVMIGGAIIW 319
            E               S    V+  QKKQRN+N+QGAYLHVLGDSIQS+GVMIGGAIIW
Sbjct: 299 DE---------------SQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIW 358

Query: 320 YKPEYKILDLICTLIFSAIVLCTTIQMLRNILEVLMESTPREIDATKLEKGLCEMDEVVA 379
           YKPE+KILDLICTL+FS IVL TTI MLRNILEVLMESTPREID T LEKG+CE++EVVA
Sbjct: 359 YKPEWKILDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIEEVVA 418

Query: 380 IHELHIWAITVGKILLACHVIIKPEANADMVLDKVIDYIRREYNISHVTIQIERQ 384
           +HELHIWAITVGK+LLACHV I+PEA ADMVLDK+IDYI+RE+NISHVTIQIERQ
Sbjct: 419 VHELHIWAITVGKLLLACHVKIRPEAEADMVLDKIIDYIKREHNISHVTIQIERQ 432

BLAST of Cp4.1LG00g02590 vs. TAIR 10
Match: AT3G61940.1 (Cation efflux family protein )

HSP 1 Score: 334.7 bits (857), Expect = 9.6e-92
Identity = 188/324 (58.02%), Postives = 222/324 (68.52%), Query Frame = 0

Query: 60  NVAAFAISLFSLWASGWEATPRQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRLING 119
           +V AFAIS+ SLWAS WEA PRQSYGFFRIEILG LVSIQ+IWLL GILVYEA+ RL+  
Sbjct: 69  DVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRLVQE 128

Query: 120 PG-EVKGLLMFAVSAFGLVVNIGMALLLGHEHGHAHSHGHSHGHGEHDHGHGKHEHGGEG 179
              +V G  M  V+AFGLVVNI M ++LGH+HGH H HGHSH HG   H +G+       
Sbjct: 129 TNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHG---HSYGE------- 188

Query: 180 HGHHHGISITTHHHHHHEGGKGAARDHHAHKEPVTTPLLEGCCEDGRSHSPKVIKMQKKQ 239
                                                            + ++++  K+ 
Sbjct: 189 ------------------------------------------------RAEQLLEKSKEI 248

Query: 240 RNINVQGAYLHVLGDSIQSIGVMIGGAIIWYKPEYKILDLICTLIFSAIVLCTTIQMLRN 299
           RNINVQGAYLHVLGD IQSIGVMIGG +IWY P++K++DLICTL FS IVL TTI+MLR+
Sbjct: 249 RNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFFSVIVLGTTIKMLRS 308

Query: 300 ILEVLMESTPREIDATKLEKGLCEMDEVVAIHELHIWAITVGKILLACHVIIKPEANADM 359
           ILEVLMESTPREIDA +LEKGL E++EVV +HELHIWAITVGK L +CHV ++PEA  +M
Sbjct: 309 ILEVLMESTPREIDARQLEKGLMEIEEVVDVHELHIWAITVGKALFSCHVKVRPEAGDEM 334

Query: 360 VLDKVIDYIRREYNISHVTIQIER 383
           VL+KVIDYI REY ISHVTIQIER
Sbjct: 369 VLNKVIDYIWREYRISHVTIQIER 334