Cp4.1LG00g01630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG00g01630 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 4.1e-163
Identity = 326/737 (44.23%), Postives = 418/737 (56.72%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIV LGSH+H  ++S+  +  V  SH   L SF+GS E AK+AIFYSYK HINGFAAIL
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 61  DEEEATNLAR-----------------------------------------------VIA 120
           DE EA  +A+                                               +IA
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 121 YL------KSHNLTDE--------------------------------------LP--LI 180
            L      +S + +DE                                      LP    
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVPCNRKLIGARYFNKGYLAYTGLPSNAS 220

Query: 181 VNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGC 240
             + RD+ GHG+HTLSTA G++V G +VFG G GTA GGSPKARVAAYKVCWP ++   C
Sbjct: 221 YETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAEC 280

Query: 241 YDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPS 300
           +D DI    + AI DGVDVLS S+G    +Y  D IAI SFHA+K G+ VVCSAGNSGP 
Sbjct: 281 FDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPK 340

Query: 301 MATATNIAPWILT-----------------------------------LYPLITGAQAKA 360
             T +N+APW++T                                   +Y LI+ A A  
Sbjct: 341 SGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANV 400

Query: 361 TTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGF 420
              +  DA+LCK  +LD  KV GKILVCL G ++R+DKGMQA  AGA GM+LCND+ SG 
Sbjct: 401 ANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGN 460

Query: 421 KIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRKLSRFVSN------- 480
           +II+D HVLPAS I Y DG+ + SY++S K+P GY+  P++    K + F+++       
Sbjct: 461 EIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPN 520

Query: 481 -----SLHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLL 540
                 L PD+TAPGV+IIAAF+ A  PT    DNR  P+ T SGTSMSCPH+SG+VGLL
Sbjct: 521 TITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLL 580

Query: 541 KALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPG 595
           K LHP WSPAAI+SAIMT++   +N    ++D     F  A PF YGSGH+ P  A  PG
Sbjct: 581 KTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPG 640

BLAST of Cp4.1LG00g01630 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.4e-150
Identity = 312/743 (41.99%), Postives = 413/743 (55.59%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SY+V  G+HSH  E++   + RV ++H+  LGSF GS E+A DAIFYSY  HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 61  DEEEATNLAR-----------------------------------------------VIA 120
           D + A  +++                                               +IA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 121 YL------KSHNLTDE-------------------------------------------L 180
            L      +S +  DE                                           L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210

Query: 181 PLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNS 240
               +S RD  GHG+HTLSTA G +V GVS+FG G GTAKGGSP+ARVAAYKVCWP +  
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270

Query: 241 GGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNS 300
             CYD D+   FD AI+DG DV+S+S+G  P  +++D++AI SFHA KK I VVCSAGNS
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330

Query: 301 GPSMATATNIAPWILTL------------------------------------YPLITGA 360
           GP+ +T +N+APW +T+                                    YP++   
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390

Query: 361 QAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDR 420
            AKA  ASA DA LCK  +LD  K  GKILVCL G + R++KG    L G +GM+L N  
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450

Query: 421 FSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK----LSRFVS 480
            +G  ++AD HVLPA+ ++  D  AVS YI+  K P+ ++ P  +    K    ++ F S
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510

Query: 481 NS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGI 540
                     L PD+TAPGV +IAA++GAVSPT E FD R + +  +SGTSMSCPH+SGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570

Query: 541 VGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGA 595
            GLLK  +P WSPAAI+SAIMT+ATI D+    I +  +     ATPF +G+GH+ P  A
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLA 630

BLAST of Cp4.1LG00g01630 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 439.9 bits (1130), Expect = 4.6e-122
Identity = 289/755 (38.28%), Postives = 373/755 (49.40%), Query Frame = 0

Query: 2   YIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAILD 61
           YIV +G+HSHG   ++ D++   DSH+ LLGS  GS EKAK+AI YSY  HINGFAA+L+
Sbjct: 32  YIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAALLE 91

Query: 62  EEEATNLAR----VIAYL-KSHNL------------------------------------ 121
           EEEA ++A+    V  +L K H L                                    
Sbjct: 92  EEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRGQNSAWQKGRFGENTIIGNIDT 151

Query: 122 --------------------------------------------------------TDEL 181
                                                                     +L
Sbjct: 152 GVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQL 211

Query: 182 PLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNS 241
             ++++ RD+VGHGTHTLSTAGG++V G  VF  G GTAKGGSP+ARVAAYKVCW   + 
Sbjct: 212 DPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTDP 271

Query: 242 GGCYDVDIFDGFDQAIYDGVDVLSLSIG----SPPEEYYDDTIAIASFHALKKGIPVVCS 301
             CY  D+    DQAI DGVDV+++S G       E  + D I+I +FHA+ K I +V S
Sbjct: 272 ASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVAS 331

Query: 302 AGNSGPSMATATNIAPWILTL----------------------------------YPLIT 361
           AGN GP+  T  N+APW+ T+                                  + LI 
Sbjct: 332 AGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINNQLIEGASLFVNLPPNQAFSLIL 391

Query: 362 GAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSR-IDKGMQAVLAGAVGMILC 421
              AK   A+  DA LC+  TLD +KVNGKI++C   G  + + +G++A+ AGA GMIL 
Sbjct: 392 STDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGMILN 451

Query: 422 NDRFSGFKIIADLHVL-----PASHISYNDGQAVSSYINSRKNPM-----------GYLF 481
           N   +G  + A+ HV      P            ++ I    +P+             LF
Sbjct: 452 NQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLF 511

Query: 482 -----PPSSKF-LRKLSRFVSNSLHPDVTAPGVDIIAAFSGAVSPTGEPFDNRT-VPYIT 541
                P  + F  R  ++   + L PDVTAPGV+I+AA+S   S +    DNR    +  
Sbjct: 512 GRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNV 571

Query: 542 MSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPAT 594
           + GTSMSCPH SGI GLLK  HP WSPAAIKSAIMT+AT  DNT   I D      A A 
Sbjct: 572 LQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA- 631

BLAST of Cp4.1LG00g01630 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 9.4e-107
Identity = 229/556 (41.19%), Postives = 315/556 (56.65%), Query Frame = 0

Query: 90  STRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGCYD 149
           S RD  GHGTHT STA GS V G S+ G   GTA+G +P+ARVA YKVCW     GGC+ 
Sbjct: 205 SPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW----LGGCFS 264

Query: 150 VDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMA 209
            DI    D+AI D V+VLS+S+G    +YY D +AI +F A+++GI V CSAGN+GPS +
Sbjct: 265 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 324

Query: 210 TATNIAPWILTL--------YPLI---------TGAQA---------------KATTASA 269
           + +N+APWI T+        +P +         TG                      ++A
Sbjct: 325 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 384

Query: 270 DDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIAD 329
            +  LC   TL   KV GKI++C  G ++R+ KG     AG VGMIL N   +G +++AD
Sbjct: 385 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 444

Query: 330 LHVLPASHISYNDGQAVSSYINSRKNPMGYL--------FPPSSKFLRKLSR----FVSN 389
            H+LPA+ +    G  +  Y+ +  NP   +          PS       SR       N
Sbjct: 445 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 504

Query: 390 SLHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHP 449
            L PD+ APGV+I+AA++GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HP
Sbjct: 505 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 564

Query: 450 EWSPAAIKSAIMTSA--TISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPGLVY 509
           EWSPAAI+SA+MT+A  T  D    L +  G     P+TPF +G+GH+ PT A +PGL+Y
Sbjct: 565 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIY 624

Query: 510 DLSPNDYLEFLCARGYTEKNMRVFAEENFKCPVSG--SILNFNYPSIGVQ-NLTGCVTLT 569
           DL+  DYL FLCA  YT   +R  +  N+ C  S   S+ + NYPS  V  +  G    T
Sbjct: 625 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 684

Query: 570 RRLKNVGRPGVYRVRV-RRPEGVKVLVKPRVLKFRKIGEEKRFELTMI--GAVAEGQIGY 594
           R + +VG  G Y V+V     GVK+ V+P VL F++  E+K + +T     +   G   +
Sbjct: 685 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSF 744

BLAST of Cp4.1LG00g01630 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 2.9e-100
Identity = 231/565 (40.88%), Postives = 312/565 (55.22%), Query Frame = 0

Query: 90  STRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGCYD 149
           S RD  GHGTHT STA GS V+  S++    GTA G + KAR+AAYK+CW    +GGCYD
Sbjct: 210 SPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW----TGGCYD 269

Query: 150 VDIFDGFDQAIYDGVDVLSLSIGS--PPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPS 209
            DI    DQA+ DGV V+SLS+G+     EY+ D+IAI +F A + GI V CSAGNSGP+
Sbjct: 270 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 329

Query: 210 MATATNIAPWILTL-----------------YPLITGAQAKATTASADDAM--------- 269
             TATNIAPWILT+                   + TG    A  +  D  +         
Sbjct: 330 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG 389

Query: 270 --LCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLH 329
             LC P  L+ S V GKI++C  GG++R++KG    LAG  GMIL N   SG ++ AD H
Sbjct: 390 SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449

Query: 330 VLPASHISYNDGQAVSSYINSRKNP------MGYLF---PPSSKFLRKLSRFVSN----S 389
           ++PA+ +    G  +  YI +  +P      +G L    PPS +     SR  ++     
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509

Query: 390 LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPE 449
           L PDV APGV+I+A ++G V PT    D R V +  +SGTSMSCPHVSG+  LL+  HP+
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPD 569

Query: 450 WSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPGLVYDLS 509
           WSPAAIKSA++T+A   +N+   I D  +     +  FI+G+GH+ P  A++PGLVYD+ 
Sbjct: 570 WSPAAIKSALVTTAYDVENSGEPIEDLATG--KSSNSFIHGAGHVDPNKALNPGLVYDIE 629

Query: 510 PNDYLEFLCARGYTEKNMRVFAE--------ENFKCPVSGSILNFNYPSIGV--QNLTGC 569
             +Y+ FLCA GY    + VF +        E  K   +G +   NYPS  V   +    
Sbjct: 630 VKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL---NYPSFSVVFASTGEV 689

Query: 570 VTLTRRLKNVGR--PGVYRVRVRRPEGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQ 594
           V   R +KNVG     VY V V+ P  V++ V P  L F K      +E+T    V  G 
Sbjct: 690 VKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGG 749

BLAST of Cp4.1LG00g01630 vs. NCBI nr
Match: KAG6583695.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1069 bits (2765), Expect = 0.0
Identity = 557/682 (81.67%), Postives = 565/682 (82.84%), Query Frame = 0

Query: 8   SHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAILDEEEATN 67
           SHSHGLEVSAIDVQRVVDSHHKLLGSFLGS EKAKDAIFYSYKNHINGFAAILDEEEATN
Sbjct: 5   SHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAAILDEEEATN 64

Query: 68  LARV------------------------------------------------IAYLKSHN 127
           LA V                                                IAYLKSHN
Sbjct: 65  LASVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYLKSHN 124

Query: 128 LTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCW 187
           LTDELPLI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCW
Sbjct: 125 LTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCW 184

Query: 188 PFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVC 247
           PF N GGCYD DIFDGFDQAIYDGVDVLSLSIGSPP+EYYDDTIAIASFHALKKGIPVVC
Sbjct: 185 PFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPVVC 244

Query: 248 SAGNSGPSMATATNIAPWILT-----------------------------------LYPL 307
           SAGNSGPSMATATNIAPWILT                                   LYPL
Sbjct: 245 SAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLYPL 304

Query: 308 ITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMIL 367
           ITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGAVGMIL
Sbjct: 305 ITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGMIL 364

Query: 368 CNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK----LS 427
           CNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYL PPSSK   K    ++
Sbjct: 365 CNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPTMA 424

Query: 428 RFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPH 487
            F S          + PDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPH
Sbjct: 425 AFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPH 484

Query: 488 VSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIH 547
           VSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT NLILDGGSP FAPATPFIYGSGHIH
Sbjct: 485 VSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGHIH 544

Query: 548 PTGAIDPGLVYDLSPNDYLEFLCARGYTEKNMRVFAEENFKCPVSGSILNFNYPSIGVQN 594
           P GAIDPGLVYDLSPNDYLEFLCA GY  KN+RVF E+NFKCPVSGSILNFNYPSIGVQN
Sbjct: 545 PIGAIDPGLVYDLSPNDYLEFLCASGYKAKNIRVFGEKNFKCPVSGSILNFNYPSIGVQN 604

BLAST of Cp4.1LG00g01630 vs. NCBI nr
Match: XP_022927103.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 563/744 (75.67%), Postives = 575/744 (77.28%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL
Sbjct: 19  SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 78

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           DEEEATNLA+                                                  
Sbjct: 79  DEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVIIA 138

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                +IAYLKS
Sbjct: 139 NLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAYLKS 198

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
           HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 199 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 258

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF N GGCYD DIFDGFDQAI+DGVDVLSLSIGSPP+EYYDDTIAIASFHALKKGIPV
Sbjct: 259 CWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPV 318

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATATNIAPWILT                                   LY
Sbjct: 319 VCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLY 378

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATT SADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 379 PLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 438

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYL PPSSK   K    
Sbjct: 439 ILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKPSPT 498

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC
Sbjct: 499 MAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 558

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNTMNLILDGGSP FAPATPFIYGSGH
Sbjct: 559 PHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYGSGH 618

BLAST of Cp4.1LG00g01630 vs. NCBI nr
Match: KAG6583696.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 563/744 (75.67%), Postives = 572/744 (76.88%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGS EKAKDAIFYSYKNHINGFAAIL
Sbjct: 26  SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSFEKAKDAIFYSYKNHINGFAAIL 85

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           DEEEATNLA+                                                  
Sbjct: 86  DEEEATNLAKHPEVATVLPNKARELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVIIA 145

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                VIAYLKS
Sbjct: 146 NLDTGVWPESKSFGEHGITGGVPSRWKGGCTDKTPDAVPCNKKLIGAKYFNQGVIAYLKS 205

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
           HNLTDELPLI NSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 206 HNLTDELPLIGNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 265

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF N GGCYD DIFDGFDQAIYDGVDVLSLSIGSPP+EYYDDTIAIASFHALKKGIPV
Sbjct: 266 CWPFRNGGGCYDADIFDGFDQAIYDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPV 325

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATATNIAPWILT                                   LY
Sbjct: 326 VCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLY 385

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV+AGAVGM
Sbjct: 386 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVVAGAVGM 445

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYL PPSSK   K    
Sbjct: 446 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPT 505

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC
Sbjct: 506 MAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 565

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNT NLILDGGSP FAPATPFIYGSGH
Sbjct: 566 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTRNLILDGGSPIFAPATPFIYGSGH 625

BLAST of Cp4.1LG00g01630 vs. NCBI nr
Match: XP_022927102.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 541/744 (72.72%), Postives = 564/744 (75.81%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSA+D+QRVVDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAAIL
Sbjct: 253 SYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAAIL 312

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           +EEEAT LA+                                                  
Sbjct: 313 EEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVIIA 372

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                +IAYLKS
Sbjct: 373 NLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYLKS 432

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
           HNLTDEL LIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 433 HNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 492

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF NSGGCYD+D+ DGFDQAI+DGVDVLSLSIGSPP EYYDDTIAIASF A+KKGIPV
Sbjct: 493 CWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGIPV 552

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMA+A+NIAPWILT                                   LY
Sbjct: 553 VCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRKLY 612

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 613 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 672

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYL PPSSK   K    
Sbjct: 673 ILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPSPT 732

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGV+IIAAFSGAVSPT EPFDNRTVPYITMSGTSMSC
Sbjct: 733 MAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSMSC 792

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNTMNLILDGGSP FAPATPFIYGSGH
Sbjct: 793 PHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGSGH 852

BLAST of Cp4.1LG00g01630 vs. NCBI nr
Match: XP_022973086.1 (subtilisin-like protease SBT5.4 [Cucurbita maxima])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 529/744 (71.10%), Postives = 539/744 (72.45%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL
Sbjct: 26  SYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 85

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           DEEEAT  A+                                                  
Sbjct: 86  DEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDVIIA 145

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                VIAYLKS
Sbjct: 146 NLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAYLKS 205

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
            NLTDELPLIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 206 QNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAAYKV 265

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF NSGGCYD DIFDG DQAIYDGVDVLSLSIGSPP EYYDDTIAIASFHALKKGIPV
Sbjct: 266 CWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKGIPV 325

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATATNIAPWILT                                   LY
Sbjct: 326 VCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLAGRKLY 385

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTASADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 386 PLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 445

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYL PPSSK   K    
Sbjct: 446 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPI 505

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGV+IIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC
Sbjct: 506 MAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 565

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNL+LDGGSP FAPATPFIYGSGH
Sbjct: 566 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYGSGH 625

BLAST of Cp4.1LG00g01630 vs. ExPASy TrEMBL
Match: A0A6J1EK27 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434038 PE=3 SV=1)

HSP 1 Score: 1064 bits (2752), Expect = 0.0
Identity = 563/744 (75.67%), Postives = 575/744 (77.28%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL
Sbjct: 19  SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 78

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           DEEEATNLA+                                                  
Sbjct: 79  DEEEATNLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFIFPFSPWRKARFGKDVIIA 138

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                +IAYLKS
Sbjct: 139 NLDTGVWPESKSFGEQGITGGVPSRWKGGCMDKTPDAVPCNKKLIGAKYFNQGLIAYLKS 198

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
           HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 199 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 258

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF N GGCYD DIFDGFDQAI+DGVDVLSLSIGSPP+EYYDDTIAIASFHALKKGIPV
Sbjct: 259 CWPFRNGGGCYDADIFDGFDQAIHDGVDVLSLSIGSPPDEYYDDTIAIASFHALKKGIPV 318

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATATNIAPWILT                                   LY
Sbjct: 319 VCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGLSLSRPLARRKLY 378

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATT SADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 379 PLITGAQAKATTTSADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 438

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHISYNDGQA+SSYINSRKNPMGYL PPSSK   K    
Sbjct: 439 ILCNDRFSGFKIIADLHVLPASHISYNDGQAISSYINSRKNPMGYLIPPSSKVNTKPSPT 498

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC
Sbjct: 499 MAAFSSRGPNIVSPEIIKPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 558

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHP+WSPAAIKSAIMTSATISDNTMNLILDGGSP FAPATPFIYGSGH
Sbjct: 559 PHVSGIVGLLKALHPQWSPAAIKSAIMTSATISDNTMNLILDGGSPIFAPATPFIYGSGH 618

BLAST of Cp4.1LG00g01630 vs. ExPASy TrEMBL
Match: A0A6J1EG71 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE=3 SV=1)

HSP 1 Score: 1015 bits (2625), Expect = 0.0
Identity = 541/744 (72.72%), Postives = 564/744 (75.81%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSA+D+QRVVDSHHKLLGS LGSSEKAKDAIF+SYKNHINGFAAIL
Sbjct: 253 SYIVLLGSHSHGLEVSAVDLQRVVDSHHKLLGSLLGSSEKAKDAIFHSYKNHINGFAAIL 312

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           +EEEAT LA+                                                  
Sbjct: 313 EEEEATKLAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGVVLPFSPWRKARFGRDVIIA 372

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                +IAYLKS
Sbjct: 373 NLDTGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDAVPCNRKLIGAKYFNQGLIAYLKS 432

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
           HNLTDEL LIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 433 HNLTDELSLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 492

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF NSGGCYD+D+ DGFDQAI+DGVDVLSLSIGSPP EYYDDTIAIASF A+KKGIPV
Sbjct: 493 CWPFHNSGGCYDIDVVDGFDQAIHDGVDVLSLSIGSPPAEYYDDTIAIASFLAIKKGIPV 552

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMA+A+NIAPWILT                                   LY
Sbjct: 553 VCSAGNSGPSMASASNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLARRKLY 612

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 613 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 672

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHI+YNDGQAV SYINS KNPMGYL PPSSK   K    
Sbjct: 673 ILCNDRFSGFKIIADLHVLPASHINYNDGQAVFSYINSWKNPMGYLIPPSSKVNTKPSPT 732

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGV+IIAAFSGAVSPT EPFDNRTVPYITMSGTSMSC
Sbjct: 733 MAAFSSRGPNMVSPEIIKPDVTAPGVNIIAAFSGAVSPTREPFDNRTVPYITMSGTSMSC 792

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSA ISDNTMNLILDGGSP FAPATPFIYGSGH
Sbjct: 793 PHVSGIVGLLKALHPEWSPAAIKSAIMTSAIISDNTMNLILDGGSPMFAPATPFIYGSGH 852

BLAST of Cp4.1LG00g01630 vs. ExPASy TrEMBL
Match: A0A6J1I7Q4 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471617 PE=3 SV=1)

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 529/744 (71.10%), Postives = 539/744 (72.45%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVS ID+QRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL
Sbjct: 26  SYIVLLGSHSHGLEVSTIDLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 85

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           DEEEAT  A+                                                  
Sbjct: 86  DEEEATKFAKHPEVAAVLPNKAKELHTTHSWEFMHLEKNGFILPFSPWRKARFGKDVIIA 145

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                VIAYLKS
Sbjct: 146 NLDTGVWPESKSFGEQGIVGGVPSRWKGGCTDKTPDAVPCNRKLIGAKYFNQGVIAYLKS 205

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
            NLTDELPLIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 206 QNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKARVAAYKV 265

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWPF NSGGCYD DIFDG DQAIYDGVDVLSLSIGSPP EYYDDTIAIASFHALKKGIPV
Sbjct: 266 CWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHALKKGIPV 325

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATATNIAPWILT                                   LY
Sbjct: 326 VCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRPLAGRKLY 385

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTASADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM
Sbjct: 386 PLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 445

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYL PPSSK   K    
Sbjct: 446 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKVNTKPSPI 505

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGV+IIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC
Sbjct: 506 MAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 565

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNL+LDGGSP FAPATPFIYGSGH
Sbjct: 566 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATPFIYGSGH 625

BLAST of Cp4.1LG00g01630 vs. ExPASy TrEMBL
Match: A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)

HSP 1 Score: 961 bits (2483), Expect = 0.0
Identity = 510/744 (68.55%), Postives = 543/744 (72.98%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIVLLGSHSHGLEVSA+D+QR VDSHHKLLGSFLGSS KAKDAIFYSYKNHINGFAAIL
Sbjct: 27  SYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSFLGSSGKAKDAIFYSYKNHINGFAAIL 86

Query: 61  DEEEATNLAR-------------------------------------------------- 120
           +EEEAT LA+                                                  
Sbjct: 87  EEEEATKLAKHPEVAAVLPNKAKELHTTHSWESMHLEKNDVIPSSSPWRKARFGEDVIIA 146

Query: 121 -----------------------------------------------------VIAYLKS 180
                                                                 +AYLKS
Sbjct: 147 NLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDKTPDGVPCNRKLIGAKYFNQGALAYLKS 206

Query: 181 HNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKV 240
            NL +ELPLIVNSTRDY GHG+HTLSTAGGSYVS VSVFGSGIGTAKGGSPKARVAAYKV
Sbjct: 207 QNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYVSNVSVFGSGIGTAKGGSPKARVAAYKV 266

Query: 241 CWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPV 300
           CWP  N GGC+D D+ + FD AI+DGVDVLSLSIGSPP EYYDD IAI SFHALKKGIPV
Sbjct: 267 CWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLSIGSPPAEYYDDIIAIGSFHALKKGIPV 326

Query: 301 VCSAGNSGPSMATATNIAPWILT-----------------------------------LY 360
           VCSAGNSGPSMATA+NIAPWILT                                   LY
Sbjct: 327 VCSAGNSGPSMATASNIAPWILTVGASTLDREFQAPIELGNGQRFTGSSLSNPLAGRKLY 386

Query: 361 PLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGM 420
           PLITGAQAKATTA A D++LCKP+TLDHSKV GKILVCL GGSSRIDKGMQA+LAGAVGM
Sbjct: 387 PLITGAQAKATTAFAQDSILCKPETLDHSKVKGKILVCLRGGSSRIDKGMQALLAGAVGM 446

Query: 421 ILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK---- 480
           ILCNDR SGF+I+ADLHVLP SHI+YNDGQAV SYI S KNPMGYL PPSSK   K    
Sbjct: 447 ILCNDRLSGFEIMADLHVLPTSHINYNDGQAVFSYIYSTKNPMGYLIPPSSKVNTKPSPT 506

Query: 481 LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSC 540
           ++ F S          + PDVTAPGV+IIAAFSGAVSPTGEPFDNR VPYITMSGTSMSC
Sbjct: 507 MAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRAVPYITMSGTSMSC 566

Query: 541 PHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGH 594
           PHVSGIVGLLKALHPEWSPAAIKSAIMTSA +SDNTMNL+LDGGSP FAPATPF+YGSGH
Sbjct: 567 PHVSGIVGLLKALHPEWSPAAIKSAIMTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGH 626

BLAST of Cp4.1LG00g01630 vs. ExPASy TrEMBL
Match: A0A6J1IAG7 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471620 PE=3 SV=1)

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 489/571 (85.64%), Postives = 498/571 (87.22%), Query Frame = 0

Query: 71  VIAYLKSHNLTDELPLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKA 130
           VIAYLKS NLTDELPLIVNSTRDYVGHG+HTLSTAGG+YVSGVSVFGSGIGTAKGGSPKA
Sbjct: 17  VIAYLKSQNLTDELPLIVNSTRDYVGHGSHTLSTAGGNYVSGVSVFGSGIGTAKGGSPKA 76

Query: 131 RVAAYKVCWPFLNSGGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHA 190
           RVAAYKVCWPF NSGGCYD DIFDG DQAIYDGVDVLSLSIGSPP EYYDDTIAIASFHA
Sbjct: 77  RVAAYKVCWPFHNSGGCYDADIFDGIDQAIYDGVDVLSLSIGSPPAEYYDDTIAIASFHA 136

Query: 191 LKKGIPVVCSAGNSGPSMATATNIAPWILT------------------------------ 250
           LKKGIPVVCSAGNSGPSMATATNIAPWILT                              
Sbjct: 137 LKKGIPVVCSAGNSGPSMATATNIAPWILTVGASTLDREFQAPIELGNGQRFWGSSLSRP 196

Query: 251 -----LYPLITGAQAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAV 310
                LYPLITGAQAKATTASADDAMLCKPKTLD SKVNGKILVCLTGGSSRIDKGMQAV
Sbjct: 197 LAGRKLYPLITGAQAKATTASADDAMLCKPKTLDRSKVNGKILVCLTGGSSRIDKGMQAV 256

Query: 311 LAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKF 370
           LAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYL PPSSK 
Sbjct: 257 LAGAVGMILCNDRFSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLIPPSSKV 316

Query: 371 LRK----LSRFVSNS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITM 430
             K    ++ F S          + PDVTAPGV+IIAAFSGAVSPTGEPFDNRTVPYITM
Sbjct: 317 NTKPSPIMAAFSSRGPNMISPEIIKPDVTAPGVNIIAAFSGAVSPTGEPFDNRTVPYITM 376

Query: 431 SGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATP 490
           SGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNL+LDGGSP FAPATP
Sbjct: 377 SGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSATISDNTMNLMLDGGSPIFAPATP 436

Query: 491 FIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCARGYTEKNMRVFAEENFKCPVSGSILNF 550
           FIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCA GY EKN+RVFA+ENFKCPV  SILNF
Sbjct: 437 FIYGSGHIHPTGAIDPGLVYDLSPNDYLEFLCASGYKEKNIRVFADENFKCPVFCSILNF 496

Query: 551 NYPSIGVQNLTGCVTLTRRLKNVGRPGVYRVRVRRPEGVKVLVKPRVLKFRKIGEEKRFE 594
           NYPSIGVQNLTG VTLTRRLKNVGRPGVYRV VRRPEGVKV VKPRVLKF KIGEEKRFE
Sbjct: 497 NYPSIGVQNLTGSVTLTRRLKNVGRPGVYRVIVRRPEGVKVSVKPRVLKFWKIGEEKRFE 556

BLAST of Cp4.1LG00g01630 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 576.2 bits (1484), Expect = 2.9e-164
Identity = 326/737 (44.23%), Postives = 418/737 (56.72%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SYIV LGSH+H  ++S+  +  V  SH   L SF+GS E AK+AIFYSYK HINGFAAIL
Sbjct: 41  SYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGFAAIL 100

Query: 61  DEEEATNLAR-----------------------------------------------VIA 120
           DE EA  +A+                                               +IA
Sbjct: 101 DENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGEDTIIA 160

Query: 121 YL------KSHNLTDE--------------------------------------LP--LI 180
            L      +S + +DE                                      LP    
Sbjct: 161 NLDTGVWPESKSFSDEGYGAVPARWKGRCHKDVPCNRKLIGARYFNKGYLAYTGLPSNAS 220

Query: 181 VNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGC 240
             + RD+ GHG+HTLSTA G++V G +VFG G GTA GGSPKARVAAYKVCWP ++   C
Sbjct: 221 YETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAEC 280

Query: 241 YDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPS 300
           +D DI    + AI DGVDVLS S+G    +Y  D IAI SFHA+K G+ VVCSAGNSGP 
Sbjct: 281 FDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPK 340

Query: 301 MATATNIAPWILT-----------------------------------LYPLITGAQAKA 360
             T +N+APW++T                                   +Y LI+ A A  
Sbjct: 341 SGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPEEKMYSLISAADANV 400

Query: 361 TTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGF 420
              +  DA+LCK  +LD  KV GKILVCL G ++R+DKGMQA  AGA GM+LCND+ SG 
Sbjct: 401 ANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGN 460

Query: 421 KIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRKLSRFVSN------- 480
           +II+D HVLPAS I Y DG+ + SY++S K+P GY+  P++    K + F+++       
Sbjct: 461 EIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPN 520

Query: 481 -----SLHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLL 540
                 L PD+TAPGV+IIAAF+ A  PT    DNR  P+ T SGTSMSCPH+SG+VGLL
Sbjct: 521 TITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLL 580

Query: 541 KALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPG 595
           K LHP WSPAAI+SAIMT++   +N    ++D     F  A PF YGSGH+ P  A  PG
Sbjct: 581 KTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPG 640

BLAST of Cp4.1LG00g01630 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 534.6 bits (1376), Expect = 9.8e-152
Identity = 312/743 (41.99%), Postives = 413/743 (55.59%), Query Frame = 0

Query: 1   SYIVLLGSHSHGLEVSAIDVQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKNHINGFAAIL 60
           SY+V  G+HSH  E++   + RV ++H+  LGSF GS E+A DAIFYSY  HINGFAA L
Sbjct: 31  SYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFAAHL 90

Query: 61  DEEEATNLAR-----------------------------------------------VIA 120
           D + A  +++                                               +IA
Sbjct: 91  DHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDTIIA 150

Query: 121 YL------KSHNLTDE-------------------------------------------L 180
            L      +S +  DE                                           L
Sbjct: 151 NLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHL 210

Query: 181 PLIVNSTRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNS 240
               +S RD  GHG+HTLSTA G +V GVS+FG G GTAKGGSP+ARVAAYKVCWP +  
Sbjct: 211 NSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKG 270

Query: 241 GGCYDVDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNS 300
             CYD D+   FD AI+DG DV+S+S+G  P  +++D++AI SFHA KK I VVCSAGNS
Sbjct: 271 NECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNS 330

Query: 301 GPSMATATNIAPWILTL------------------------------------YPLITGA 360
           GP+ +T +N+APW +T+                                    YP++   
Sbjct: 331 GPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASV 390

Query: 361 QAKATTASADDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDR 420
            AKA  ASA DA LCK  +LD  K  GKILVCL G + R++KG    L G +GM+L N  
Sbjct: 391 NAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTY 450

Query: 421 FSGFKIIADLHVLPASHISYNDGQAVSSYINSRKNPMGYLFPPSSKFLRK----LSRFVS 480
            +G  ++AD HVLPA+ ++  D  AVS YI+  K P+ ++ P  +    K    ++ F S
Sbjct: 451 VTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSS 510

Query: 481 NS--------LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGI 540
                     L PD+TAPGV +IAA++GAVSPT E FD R + +  +SGTSMSCPH+SGI
Sbjct: 511 KGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGI 570

Query: 541 VGLLKALHPEWSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGA 595
            GLLK  +P WSPAAI+SAIMT+ATI D+    I +  +     ATPF +G+GH+ P  A
Sbjct: 571 AGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLA 630

BLAST of Cp4.1LG00g01630 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.6e-108
Identity = 229/556 (41.19%), Postives = 315/556 (56.65%), Query Frame = 0

Query: 90  STRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGCYD 149
           S RD  GHGTHT STA GS V G S+ G   GTA+G +P+ARVA YKVCW     GGC+ 
Sbjct: 205 SPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW----LGGCFS 264

Query: 150 VDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMA 209
            DI    D+AI D V+VLS+S+G    +YY D +AI +F A+++GI V CSAGN+GPS +
Sbjct: 265 SDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSS 324

Query: 210 TATNIAPWILTL--------YPLI---------TGAQA---------------KATTASA 269
           + +N+APWI T+        +P +         TG                      ++A
Sbjct: 325 SLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNA 384

Query: 270 DDAMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIAD 329
            +  LC   TL   KV GKI++C  G ++R+ KG     AG VGMIL N   +G +++AD
Sbjct: 385 TNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVAD 444

Query: 330 LHVLPASHISYNDGQAVSSYINSRKNPMGYL--------FPPSSKFLRKLSR----FVSN 389
            H+LPA+ +    G  +  Y+ +  NP   +          PS       SR       N
Sbjct: 445 AHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPN 504

Query: 390 SLHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHP 449
            L PD+ APGV+I+AA++GA  PTG   D+R V +  +SGTSMSCPHVSG+  LLK++HP
Sbjct: 505 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 564

Query: 450 EWSPAAIKSAIMTSA--TISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPGLVY 509
           EWSPAAI+SA+MT+A  T  D    L +  G     P+TPF +G+GH+ PT A +PGL+Y
Sbjct: 565 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGK----PSTPFDHGAGHVSPTTATNPGLIY 624

Query: 510 DLSPNDYLEFLCARGYTEKNMRVFAEENFKCPVSG--SILNFNYPSIGVQ-NLTGCVTLT 569
           DL+  DYL FLCA  YT   +R  +  N+ C  S   S+ + NYPS  V  +  G    T
Sbjct: 625 DLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYT 684

Query: 570 RRLKNVGRPGVYRVRV-RRPEGVKVLVKPRVLKFRKIGEEKRFELTMI--GAVAEGQIGY 594
           R + +VG  G Y V+V     GVK+ V+P VL F++  E+K + +T     +   G   +
Sbjct: 685 RTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSF 744

BLAST of Cp4.1LG00g01630 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 367.5 bits (942), Expect = 2.1e-101
Identity = 222/555 (40.00%), Postives = 306/555 (55.14%), Query Frame = 0

Query: 90  STRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGCYD 149
           S RD  GHGTHT +TA GS V   S  G   GTA+G + +ARVA YKVCW    S GC+ 
Sbjct: 201 SPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCW----STGCFG 260

Query: 150 VDIFDGFDQAIYDGVDVLSLSIGSPPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPSMA 209
            DI    D+AI DGVDVLSLS+G     YY DTIAI +F A+++G+ V CSAGNSGP+ A
Sbjct: 261 SDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRA 320

Query: 210 TATNIAPWILTL--------YPL---------ITGAQAKA-------------TTASADD 269
           +  N+APW++T+        +P          +TG    +                ++  
Sbjct: 321 SVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSS 380

Query: 270 AMLCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLH 329
           + LC P +LD S V GKI+VC  G ++R++KG     AG +GMI+ N   SG +++AD H
Sbjct: 381 SNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 440

Query: 330 VLPASHISYNDGQAVSSYINSRKNPMGYL--------FPPSSKFLRKLSR----FVSNSL 389
           +LPA  +    G  +  Y+ S   P   L          PS       SR         L
Sbjct: 441 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEIL 500

Query: 390 HPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPEW 449
            PDV  PGV+I+A +S A+ PTG   D+R   +  MSGTSMSCPH+SG+ GLLKA HPEW
Sbjct: 501 KPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMSCPHISGLAGLLKAAHPEW 560

Query: 450 SPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPGLVYDLSP 509
           SP+AIKSA+MT+A + DNT   + D      + + P+ +GSGH+ P  A+ PGLVYD+S 
Sbjct: 561 SPSAIKSALMTTAYVLDNTNAPLHDAADN--SLSNPYAHGSGHVDPQKALSPGLVYDIST 620

Query: 510 NDYLEFLCARGYT-EKNMRVFAEENFKCPVSGSIL-NFNYPSIGVQNLTG---CVTLTRR 569
            +Y+ FLC+  YT +  + +    +  C    S     NYPS  V  L G    V  TR 
Sbjct: 621 EEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFSV--LFGGKRVVRYTRE 680

Query: 570 LKNVG-RPGVYRVRVRRPEGVKVLVKPRVLKFRKIGEEKRFELTMI---GAVAEGQIGYG 594
           + NVG    VY+V V     V + VKP  L F+ +GE+KR+ +T +   G     +  +G
Sbjct: 681 VTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEFG 740

BLAST of Cp4.1LG00g01630 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 367.5 bits (942), Expect = 2.1e-101
Identity = 231/565 (40.88%), Postives = 312/565 (55.22%), Query Frame = 0

Query: 90  STRDYVGHGTHTLSTAGGSYVSGVSVFGSGIGTAKGGSPKARVAAYKVCWPFLNSGGCYD 149
           S RD  GHGTHT STA GS V+  S++    GTA G + KAR+AAYK+CW    +GGCYD
Sbjct: 210 SPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICW----TGGCYD 269

Query: 150 VDIFDGFDQAIYDGVDVLSLSIGS--PPEEYYDDTIAIASFHALKKGIPVVCSAGNSGPS 209
            DI    DQA+ DGV V+SLS+G+     EY+ D+IAI +F A + GI V CSAGNSGP+
Sbjct: 270 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 329

Query: 210 MATATNIAPWILTL-----------------YPLITGAQAKATTASADDAM--------- 269
             TATNIAPWILT+                   + TG    A  +  D  +         
Sbjct: 330 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG 389

Query: 270 --LCKPKTLDHSKVNGKILVCLTGGSSRIDKGMQAVLAGAVGMILCNDRFSGFKIIADLH 329
             LC P  L+ S V GKI++C  GG++R++KG    LAG  GMIL N   SG ++ AD H
Sbjct: 390 SRLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSH 449

Query: 330 VLPASHISYNDGQAVSSYINSRKNP------MGYLF---PPSSKFLRKLSRFVSN----S 389
           ++PA+ +    G  +  YI +  +P      +G L    PPS +     SR  ++     
Sbjct: 450 LVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHLTPVI 509

Query: 390 LHPDVTAPGVDIIAAFSGAVSPTGEPFDNRTVPYITMSGTSMSCPHVSGIVGLLKALHPE 449
           L PDV APGV+I+A ++G V PT    D R V +  +SGTSMSCPHVSG+  LL+  HP+
Sbjct: 510 LKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPD 569

Query: 450 WSPAAIKSAIMTSATISDNTMNLILDGGSPFFAPATPFIYGSGHIHPTGAIDPGLVYDLS 509
           WSPAAIKSA++T+A   +N+   I D  +     +  FI+G+GH+ P  A++PGLVYD+ 
Sbjct: 570 WSPAAIKSALVTTAYDVENSGEPIEDLATG--KSSNSFIHGAGHVDPNKALNPGLVYDIE 629

Query: 510 PNDYLEFLCARGYTEKNMRVFAE--------ENFKCPVSGSILNFNYPSIGV--QNLTGC 569
             +Y+ FLCA GY    + VF +        E  K   +G +   NYPS  V   +    
Sbjct: 630 VKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDL---NYPSFSVVFASTGEV 689

Query: 570 VTLTRRLKNVGR--PGVYRVRVRRPEGVKVLVKPRVLKFRKIGEEKRFELTMIGAVAEGQ 594
           V   R +KNVG     VY V V+ P  V++ V P  L F K      +E+T    V  G 
Sbjct: 690 VKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGG 749