CmoCh20G011450 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh20G011450 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 4.8e-88
Identity = 162/341 (47.51%), Postives = 226/341 (66.28%), Query Frame = 0

Query: 36  SSENRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLA 95
           SS+  +  FIFGDS  D GNNN+INT  +F ANF PYG+++F++PTGRFSDGRIIPDF+A
Sbjct: 27  SSQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIA 86

Query: 96  EYANLPLIQPYLDPKNNLYLNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTK 155
           EYA+LP+I  YL+P NN + +G NFAS G GAL  SH G A+GLQTQ+++F  + +   +
Sbjct: 87  EYASLPIIPAYLEP-NNDFTHGANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQ 146

Query: 156 RLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEV 215
            LG+ +++   S++V+LF+ GGNDY +P+   Y        QEQ+ D+VIGN+T  +K +
Sbjct: 147 NLGDIKSRQLLSDAVYLFSCGGNDYQSPY---YPY-----TQEQYVDIVIGNMTNVIKGI 206

Query: 216 YKCGGRKFGFMTVPPLGYTPSSRLKKNAQFFD-ETSSLARVHNKLFLMAIQRLATQLDGF 275
           Y+ GGRKFG + VP +G  P  R K+     + E   L R+HN+ F   +++L  QL+GF
Sbjct: 207 YEKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGF 266

Query: 276 KYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDY 335
            YA  D  T +L R+ NP+KYGFK  ++ACCGS  F G ++CGR K    + LC+N  +Y
Sbjct: 267 VYAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRIK---EFGLCDNATEY 326

Query: 336 MFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQYDP 376
            FFD +HP E    Q A+  W GD+ + +PYN K LF+  P
Sbjct: 327 FFFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALFEGKP 355

BLAST of CmoCh20G011450 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 4.9e-85
Identity = 162/340 (47.65%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 39  NRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYA 98
           N+S  F+FGDS  DAGNNNYI+T     +N+ PYG+T F +PTGR SDGR+IPDF+AEYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 99  NLPLIQPYLDP--KNNLYLNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKR 158
            LPLI P L P   N+ +  GVNFASGG GAL G+  G  I L+TQ+  FK+V+  L  +
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 159 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVY 218
           LG+A  +   S +V+LF+ G NDY  PF  +  +F+++ + E++ D V+GN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVY 213

Query: 219 KCGGRKFGFMTVPPLGYTPSSRL---KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDG 278
             GGRKFG +   P    P+S +    K    F   + L  +HN+  L  ++RL  +L G
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 279 FKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKG-AIPYTLCENVQ 338
           FKYA  D HT L +R+ +P+KYGFK    ACCGS   RG   CG + G +  Y LCENV 
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVT 333

Query: 339 DYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQ 373
           DY+FFD +H TEK   Q+A+ +WSG  +I  PYN K LF+
Sbjct: 334 DYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of CmoCh20G011450 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 9.3e-84
Identity = 161/340 (47.35%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 39  NRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYA 98
           N+S  F+FGDS  DAGNNNYI+T   F +N+ PYG+T F  PTGR SDGR IPDF+AEYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 99  NLPLIQPYLDPKN--NLYLNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKR 158
            LPLI  YL P N  N +  GV+FAS G GAL G+  G  I L++Q+  FK+V+  L   
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 159 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVY 218
           LG A+ +   S +V+LF+ G NDY  PF  +  IF++   QE + D V+GN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVY 215

Query: 219 KCGGRKFGFMTVPPLGYTPSSRL---KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDG 278
           K GGRKFGF+ +      P+S +    K    F   + L  +HN+     ++RL  +L G
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 279 FKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKG-AIPYTLCENVQ 338
           FKYA  D HT L  R+ NP+KYGFK    ACCG+   RG   CG + G +  Y LCE V 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 339 DYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQ 373
           DY+FFD +H TEK  +Q+A+ +WSG  ++ KPYN + LF+
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFE 374

BLAST of CmoCh20G011450 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 305.1 bits (780), Expect = 1.1e-81
Identity = 163/379 (43.01%), Postives = 233/379 (61.48%), Query Frame = 0

Query: 2   KISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINT 61
           K+++     F FFI+       +G    ++S      N +  F+FGDS++DAGNNNYINT
Sbjct: 9   KVTRRTISSFIFFIVSSTILFLAGKSSAKISH-NGDNNVTALFLFGDSFLDAGNNNYINT 68

Query: 62  TDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNN-LYLNGVNF 121
           T    ANFPPYG+TFF  PTGRFSDGR+I DF+AEYANLPLI P+L+P N+   L GVNF
Sbjct: 69  TTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNF 128

Query: 122 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 181
           AS G GAL  + QG  I L+TQ+  +K+V+       G   ++   S +V+L + G NDY
Sbjct: 129 ASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDY 188

Query: 182 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRL- 241
            + F  +  +  ++    Q  D+VIGN+T  + E+YK GGRKFGF+ VP LG  P+ R+ 
Sbjct: 189 SSIFLTNQSLPISM---SQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRIL 248

Query: 242 --KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGF 301
             K +     + S LA +HN+     + ++  Q+ GFK++  D +  L  R+ +P+K+GF
Sbjct: 249 QPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGF 308

Query: 302 KIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSG 361
           K  + ACCG+ ++RG F+CG K+    Y LCEN +DY+F+DS H T+ T+ Q A  +W+G
Sbjct: 309 KEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNG 368

Query: 362 ----DAHIVKPYNFKQLFQ 373
               D+ +V PYN   LFQ
Sbjct: 369 GHMSDSLVVGPYNINNLFQ 383

BLAST of CmoCh20G011450 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 303.9 bits (777), Expect = 2.5e-81
Identity = 169/369 (45.80%), Postives = 221/369 (59.89%), Query Frame = 0

Query: 10  FFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINTTDDFLANF 69
           FF + I+L I +         + +     N++  F+FGDS  DAGNNNYINT   F +N 
Sbjct: 9   FFVYTIILSIGSINC------IDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNI 68

Query: 70  PPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPK--NNLYLNGVNFASGGGGA 129
            PYG+T F  PTGR SDG        E A LP I P L P   NN +  GV+FAS G GA
Sbjct: 69  WPYGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGA 128

Query: 130 LAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFDIS 189
           LA S  G  I L TQ+  FK V+ SL   LG+A T+  FS +V+LF+ G NDY  PF  +
Sbjct: 129 LAESFLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSAN 188

Query: 190 YDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK---KNAQ 249
              FK+ +++E+F D VIGNIT  ++EVYK GGRKFGF+ V P   +P+S ++   K   
Sbjct: 189 SSTFKS-NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGS 248

Query: 250 FFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTAC 309
            F   + L  +HNK F   ++RL  QL GF+YA  D HT L +RI +P+KYGFK    AC
Sbjct: 249 CFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKAC 308

Query: 310 CGSDEFRGFFNCGRKKG-AIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVK 369
           CGS   RG   CG + G +  Y LCENV DY+F+DS H TEK   Q+A+ +W+G  ++ +
Sbjct: 309 CGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTR 363

Query: 370 PYNFKQLFQ 373
           PYN K LF+
Sbjct: 369 PYNLKALFE 363

BLAST of CmoCh20G011450 vs. ExPASy TrEMBL
Match: A0A6J1EJ18 (GDSL esterase/lipase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111434897 PE=3 SV=1)

HSP 1 Score: 782.3 bits (2019), Expect = 9.0e-223
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60
           MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN
Sbjct: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120
           TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF
Sbjct: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120

Query: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180
           ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY
Sbjct: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180

Query: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240
           LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK
Sbjct: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240

Query: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300
           KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV
Sbjct: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300

Query: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360
           DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH
Sbjct: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360

Query: 361 IVKPYNFKQLFQYDPFLGSQ 381
           IVKPYNFKQLFQYDPFLGSQ
Sbjct: 361 IVKPYNFKQLFQYDPFLGSQ 380

BLAST of CmoCh20G011450 vs. ExPASy TrEMBL
Match: A0A6J1I3Y7 (GDSL esterase/lipase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111470363 PE=3 SV=1)

HSP 1 Score: 731.5 bits (1887), Expect = 1.8e-207
Identity = 357/380 (93.95%), Postives = 367/380 (96.58%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60
           MKISKMGS FFFFFILLG AAAGSGFPGIQL DIPSSENRSGFFIFGDSYVDAGNNNYIN
Sbjct: 1   MKISKMGS-FFFFFILLGNAAAGSGFPGIQLPDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120
           TT DFLAN+PPYGETFFTTPTGRFSDGRIIPDFLAEYA LPLIQPYLDPKNNLYLNGVNF
Sbjct: 61  TTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNLYLNGVNF 120

Query: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180
           ASGGGGALA SHQGQAIGLQTQMKFFKRVKNSLTKRLG+ART+SFFSNSVFLFNFGGNDY
Sbjct: 121 ASGGGGALAESHQGQAIGLQTQMKFFKRVKNSLTKRLGSARTRSFFSNSVFLFNFGGNDY 180

Query: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240
           LNPFDISYDIFKTLDAQEQFA+MVIGNITIALKEVYKCGGRKFGFMTVPP GYTPSSRLK
Sbjct: 181 LNPFDISYDIFKTLDAQEQFANMVIGNITIALKEVYKCGGRKFGFMTVPPFGYTPSSRLK 240

Query: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300
           K+AQFF ETSSLARVHNKLFLMAIQRLATQL GFKYAFADTHTLLL+RILNPTKYGFKI+
Sbjct: 241 KSAQFFYETSSLARVHNKLFLMAIQRLATQLHGFKYAFADTHTLLLRRILNPTKYGFKII 300

Query: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360
           DTACCGSDEFRGFFNCGRKKGA+PYTLCEN+QDYMFFDSYHPTEKTFEQLA EMWSGDA 
Sbjct: 301 DTACCGSDEFRGFFNCGRKKGALPYTLCENIQDYMFFDSYHPTEKTFEQLANEMWSGDAD 360

Query: 361 IVKPYNFKQLFQYDPFLGSQ 381
           IVK YNFKQLFQYDPFL +Q
Sbjct: 361 IVKSYNFKQLFQYDPFLDAQ 379

BLAST of CmoCh20G011450 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 4.2e-164
Identity = 285/381 (74.80%), Postives = 320/381 (83.99%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFP------GIQLSDIPSSENRSGFFIFGDSYVDAG 60
           MK   +GS FFF      + AA  GFP      GIQL   PSSENR GFFIFGDSYVD G
Sbjct: 1   MKFPNLGSCFFFI-----LFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVG 60

Query: 61  NNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLY 120
           NNNYINTT DF ANFPPYGE+FF   TGRF+DGR IPDFL EYANLPL+ PYLDP N+LY
Sbjct: 61  NNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLY 120

Query: 121 LNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFN 180
             GVNFASGGGGALA SHQ QA+GLQTQMKFFKRV  SL K+LGNARTQSFFSNSVFLFN
Sbjct: 121 DYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFN 180

Query: 181 FGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYT 240
           FGGNDYLNPFDISYDIFKT+DAQEQF +MVIGNIT ALKEVYK GGRKFG M VPPLGY 
Sbjct: 181 FGGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYM 240

Query: 241 PSSRLKKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTK 300
           PSSRLKK+AQFF+E SS+AR+HNKL  +A+ +L+ QL GFKYAFADTHTLLLQRILNP++
Sbjct: 241 PSSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSR 300

Query: 301 YGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEM 360
           YGFK+VDTACCGSDEFRG +NCGRK G++P+T C+N++D+MFFDS+HPTEK F+QL ++M
Sbjct: 301 YGFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQM 360

Query: 361 WSGDAHIVKPYNFKQLFQYDP 376
           WSG   +VKPYNFKQLF Y+P
Sbjct: 361 WSGGLEVVKPYNFKQLFDYEP 376

BLAST of CmoCh20G011450 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.4e-161
Identity = 280/386 (72.54%), Postives = 316/386 (81.87%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFP------GIQLSDIPSSENRSGFFIFGDSYVDAG 60
           MK     S FFF   +     A  GFP      GIQL+  PSSENR GFFIFGDSYVDAG
Sbjct: 1   MKFPNRSSCFFFILFV-----AQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVDAG 60

Query: 61  NNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLY 120
           NNNYINTT DF ANFPPYGE+FF   TGRF+DGR IPDFL EYANLPL+ PYLDP N+LY
Sbjct: 61  NNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLY 120

Query: 121 LNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFN 180
             GVNFASGGGGAL  SHQ QA+GLQTQM FFKRV  SL K+LGNARTQSFFSNSVFLFN
Sbjct: 121 DYGVNFASGGGGALPLSHQEQALGLQTQMNFFKRVDKSLRKKLGNARTQSFFSNSVFLFN 180

Query: 181 FGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYT 240
           FGGNDYLNPFDISYDIFKT+D QEQF +MVIGNIT  LKEVYK GGRKFG M VPPLGY 
Sbjct: 181 FGGNDYLNPFDISYDIFKTIDTQEQFVNMVIGNITTVLKEVYKHGGRKFGLMGVPPLGYM 240

Query: 241 PSSRLKKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTK 300
           PSSRLKK+AQ+F+E SS+AR+HNKL  +A+ +L+ QL GFKYAFAD H LLLQRILNP++
Sbjct: 241 PSSRLKKSAQYFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADVHMLLLQRILNPSQ 300

Query: 301 YGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEM 360
           YGFK+VDTACCGSDEFRG +NCGRK G+ P+T C+N++D+MFFDSYHPTEK F+QLA++M
Sbjct: 301 YGFKVVDTACCGSDEFRGVYNCGRKLGSFPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQM 360

Query: 361 WSGDAHIVKPYNFKQLFQYDPFLGSQ 381
           WSG   +V PYNFKQLF+Y+P L S+
Sbjct: 361 WSGGLEVVTPYNFKQLFEYEPTLASE 381

BLAST of CmoCh20G011450 vs. ExPASy TrEMBL
Match: A0A6J1G6L0 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451341 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 6.8e-154
Identity = 265/379 (69.92%), Postives = 304/379 (80.21%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60
           MK+  +G  FFFFFIL  +  A   FP      +P   N   FF+FGDSYVDAGNNNYIN
Sbjct: 1   MKLPNLGFCFFFFFILFALHTASPTFPEATTIPLP---NCFAFFLFGDSYVDAGNNNYIN 60

Query: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120
           TT DF ANFPPYGETFF  PT RF+DGR IPDFLAEYA LPLI PYLDP  N Y NGVNF
Sbjct: 61  TTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDNGVNF 120

Query: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180
           AS G GALA SH+G AIGLQTQMKFFK+V  SL K+LGNA  Q+  SNS+FLFNFGGNDY
Sbjct: 121 ASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFGGNDY 180

Query: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240
           LNPFDISYDIF T  AQE+F +MV+GNITIALKEVYK GGRKFGFMTVPPLG+ PSSR+K
Sbjct: 181 LNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPSSRIK 240

Query: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300
           ++ QFF+E SS+ R+HN+L   A+Q+L  QL+GFKYAFADTHTLLLQRILNP KYGFK+V
Sbjct: 241 RSVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYGFKVV 300

Query: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360
           +TACCGS  FRG +NCGR    +P+T C+N++DY+FFDS+HPTEK F+QLA+ +WSGDA 
Sbjct: 301 ETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWSGDAQ 360

Query: 361 IVKPYNFKQLFQYDPFLGS 380
           IVKPYNFKQLF+YD  L S
Sbjct: 361 IVKPYNFKQLFEYDSMLAS 376

BLAST of CmoCh20G011450 vs. NCBI nr
Match: XP_022927996.1 (GDSL esterase/lipase 1-like [Cucurbita moschata])

HSP 1 Score: 782.3 bits (2019), Expect = 1.9e-222
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60
           MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN
Sbjct: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120
           TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF
Sbjct: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120

Query: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180
           ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY
Sbjct: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180

Query: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240
           LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK
Sbjct: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240

Query: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300
           KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV
Sbjct: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300

Query: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360
           DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH
Sbjct: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360

Query: 361 IVKPYNFKQLFQYDPFLGSQ 381
           IVKPYNFKQLFQYDPFLGSQ
Sbjct: 361 IVKPYNFKQLFQYDPFLGSQ 380

BLAST of CmoCh20G011450 vs. NCBI nr
Match: XP_023512753.1 (GDSL esterase/lipase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 745.0 bits (1922), Expect = 3.3e-211
Identity = 364/376 (96.81%), Postives = 368/376 (97.87%), Query Frame = 0

Query: 6   MGSFFFFF-FILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINTTDD 65
           MGSFFFFF FILLGIAAA SGFPGIQL  IPSSENRSGFFIFGDSYVDAGNNNYINTT D
Sbjct: 1   MGSFFFFFVFILLGIAAAESGFPGIQLPGIPSSENRSGFFIFGDSYVDAGNNNYINTTGD 60

Query: 66  FLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNFASGG 125
           FLAN+PPYGETFF TPTGRFSDGRIIPDFLAEYANLPLIQPYLDP NNLYLNGVNFASGG
Sbjct: 61  FLANYPPYGETFFPTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPTNNLYLNGVNFASGG 120

Query: 126 GGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPF 185
           GGALAGSH+GQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPF
Sbjct: 121 GGALAGSHKGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPF 180

Query: 186 DISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNAQ 245
           DISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNAQ
Sbjct: 181 DISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNAQ 240

Query: 246 FFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTAC 305
           FFDE+SSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTAC
Sbjct: 241 FFDESSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTAC 300

Query: 306 CGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKP 365
           CGSDEFRGFFNCGRKKGA+PYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDA IVKP
Sbjct: 301 CGSDEFRGFFNCGRKKGALPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDADIVKP 360

Query: 366 YNFKQLFQYDPFLGSQ 381
           YNFKQLFQYDPFLGSQ
Sbjct: 361 YNFKQLFQYDPFLGSQ 376

BLAST of CmoCh20G011450 vs. NCBI nr
Match: XP_022971691.1 (GDSL esterase/lipase 1-like [Cucurbita maxima])

HSP 1 Score: 731.5 bits (1887), Expect = 3.8e-207
Identity = 357/380 (93.95%), Postives = 367/380 (96.58%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYIN 60
           MKISKMGS FFFFFILLG AAAGSGFPGIQL DIPSSENRSGFFIFGDSYVDAGNNNYIN
Sbjct: 1   MKISKMGS-FFFFFILLGNAAAGSGFPGIQLPDIPSSENRSGFFIFGDSYVDAGNNNYIN 60

Query: 61  TTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNF 120
           TT DFLAN+PPYGETFFTTPTGRFSDGRIIPDFLAEYA LPLIQPYLDPKNNLYLNGVNF
Sbjct: 61  TTGDFLANYPPYGETFFTTPTGRFSDGRIIPDFLAEYAKLPLIQPYLDPKNNLYLNGVNF 120

Query: 121 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 180
           ASGGGGALA SHQGQAIGLQTQMKFFKRVKNSLTKRLG+ART+SFFSNSVFLFNFGGNDY
Sbjct: 121 ASGGGGALAESHQGQAIGLQTQMKFFKRVKNSLTKRLGSARTRSFFSNSVFLFNFGGNDY 180

Query: 181 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK 240
           LNPFDISYDIFKTLDAQEQFA+MVIGNITIALKEVYKCGGRKFGFMTVPP GYTPSSRLK
Sbjct: 181 LNPFDISYDIFKTLDAQEQFANMVIGNITIALKEVYKCGGRKFGFMTVPPFGYTPSSRLK 240

Query: 241 KNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIV 300
           K+AQFF ETSSLARVHNKLFLMAIQRLATQL GFKYAFADTHTLLL+RILNPTKYGFKI+
Sbjct: 241 KSAQFFYETSSLARVHNKLFLMAIQRLATQLHGFKYAFADTHTLLLRRILNPTKYGFKII 300

Query: 301 DTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAH 360
           DTACCGSDEFRGFFNCGRKKGA+PYTLCEN+QDYMFFDSYHPTEKTFEQLA EMWSGDA 
Sbjct: 301 DTACCGSDEFRGFFNCGRKKGALPYTLCENIQDYMFFDSYHPTEKTFEQLANEMWSGDAD 360

Query: 361 IVKPYNFKQLFQYDPFLGSQ 381
           IVK YNFKQLFQYDPFL +Q
Sbjct: 361 IVKSYNFKQLFQYDPFLDAQ 379

BLAST of CmoCh20G011450 vs. NCBI nr
Match: XP_023513196.1 (GDSL esterase/lipase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 651.4 bits (1679), Expect = 4.9e-183
Identity = 329/377 (87.27%), Postives = 336/377 (89.12%), Query Frame = 0

Query: 6   MGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINTTDDF 65
           MGS  FFFFILLGI AAGSGFPGIQL  I SSENR GFFIFGDSYVDAGNNNYINTT DF
Sbjct: 1   MGS--FFFFILLGIGAAGSGFPGIQLPGITSSENRFGFFIFGDSYVDAGNNNYINTTGDF 60

Query: 66  LA--NFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLYLNGVNFASG 125
           LA  NF                         AEYA LPLIQPYLDPKNNLYLNGVNFASG
Sbjct: 61  LAKKNF-----------------------IAAEYAKLPLIQPYLDPKNNLYLNGVNFASG 120

Query: 126 GGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNP 185
           GGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNP
Sbjct: 121 GGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNP 180

Query: 186 FDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLKKNA 245
           FDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPP+GY PSSRLKK+A
Sbjct: 181 FDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPMGYMPSSRLKKSA 240

Query: 246 QFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTA 305
           QFF+ETSSLARVHNKL LMAIQRLATQL GFKYAFADTHTLLL++ILNPTKYGFKIVDTA
Sbjct: 241 QFFEETSSLARVHNKLLLMAIQRLATQLHGFKYAFADTHTLLLEKILNPTKYGFKIVDTA 300

Query: 306 CCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVK 365
           CCGSDEFRGFFNCGRKKGA+PYTLCENVQDYMFFDSYHPTEKTFEQLAKEM SGDA +VK
Sbjct: 301 CCGSDEFRGFFNCGRKKGALPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMSSGDADVVK 352

Query: 366 PYNFKQLFQYDPFLGSQ 381
           PYNFKQLFQYDPFLGSQ
Sbjct: 361 PYNFKQLFQYDPFLGSQ 352

BLAST of CmoCh20G011450 vs. NCBI nr
Match: XP_022928027.1 (GDSL esterase/lipase 3-like [Cucurbita moschata])

HSP 1 Score: 587.4 bits (1513), Expect = 8.7e-164
Identity = 285/381 (74.80%), Postives = 320/381 (83.99%), Query Frame = 0

Query: 1   MKISKMGSFFFFFFILLGIAAAGSGFP------GIQLSDIPSSENRSGFFIFGDSYVDAG 60
           MK   +GS FFF      + AA  GFP      GIQL   PSSENR GFFIFGDSYVD G
Sbjct: 1   MKFPNLGSCFFFI-----LFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVG 60

Query: 61  NNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNNLY 120
           NNNYINTT DF ANFPPYGE+FF   TGRF+DGR IPDFL EYANLPL+ PYLDP N+LY
Sbjct: 61  NNNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLY 120

Query: 121 LNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFN 180
             GVNFASGGGGALA SHQ QA+GLQTQMKFFKRV  SL K+LGNARTQSFFSNSVFLFN
Sbjct: 121 DYGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFN 180

Query: 181 FGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYT 240
           FGGNDYLNPFDISYDIFKT+DAQEQF +MVIGNIT ALKEVYK GGRKFG M VPPLGY 
Sbjct: 181 FGGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYM 240

Query: 241 PSSRLKKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTK 300
           PSSRLKK+AQFF+E SS+AR+HNKL  +A+ +L+ QL GFKYAFADTHTLLLQRILNP++
Sbjct: 241 PSSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSR 300

Query: 301 YGFKIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEM 360
           YGFK+VDTACCGSDEFRG +NCGRK G++P+T C+N++D+MFFDS+HPTEK F+QL ++M
Sbjct: 301 YGFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQM 360

Query: 361 WSGDAHIVKPYNFKQLFQYDP 376
           WSG   +VKPYNFKQLF Y+P
Sbjct: 361 WSGGLEVVKPYNFKQLFDYEP 376

BLAST of CmoCh20G011450 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 316.2 bits (809), Expect = 3.5e-86
Identity = 162/340 (47.65%), Postives = 216/340 (63.53%), Query Frame = 0

Query: 39  NRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYA 98
           N+S  F+FGDS  DAGNNNYI+T     +N+ PYG+T F +PTGR SDGR+IPDF+AEYA
Sbjct: 34  NQSALFVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYA 93

Query: 99  NLPLIQPYLDP--KNNLYLNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKR 158
            LPLI P L P   N+ +  GVNFASGG GAL G+  G  I L+TQ+  FK+V+  L  +
Sbjct: 94  WLPLIPPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSK 153

Query: 159 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVY 218
           LG+A  +   S +V+LF+ G NDY  PF  +  +F+++ + E++ D V+GN+T   KEVY
Sbjct: 154 LGDAEGKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVY 213

Query: 219 KCGGRKFGFMTVPPLGYTPSSRL---KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDG 278
             GGRKFG +   P    P+S +    K    F   + L  +HN+  L  ++RL  +L G
Sbjct: 214 NLGGRKFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSG 273

Query: 279 FKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKG-AIPYTLCENVQ 338
           FKYA  D HT L +R+ +P+KYGFK    ACCGS   RG   CG + G +  Y LCENV 
Sbjct: 274 FKYALHDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVT 333

Query: 339 DYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQ 373
           DY+FFD +H TEK   Q+A+ +WSG  +I  PYN K LF+
Sbjct: 334 DYLFFDPFHLTEKANRQIAELIWSGPTNITGPYNLKALFE 372

BLAST of CmoCh20G011450 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 305.1 bits (780), Expect = 8.1e-83
Identity = 163/379 (43.01%), Postives = 233/379 (61.48%), Query Frame = 0

Query: 2   KISKMGSFFFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINT 61
           K+++     F FFI+       +G    ++S      N +  F+FGDS++DAGNNNYINT
Sbjct: 9   KVTRRTISSFIFFIVSSTILFLAGKSSAKISH-NGDNNVTALFLFGDSFLDAGNNNYINT 68

Query: 62  TDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPKNN-LYLNGVNF 121
           T    ANFPPYG+TFF  PTGRFSDGR+I DF+AEYANLPLI P+L+P N+   L GVNF
Sbjct: 69  TTLDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNF 128

Query: 122 ASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDY 181
           AS G GAL  + QG  I L+TQ+  +K+V+       G   ++   S +V+L + G NDY
Sbjct: 129 ASAGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDY 188

Query: 182 LNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRL- 241
            + F  +  +  ++    Q  D+VIGN+T  + E+YK GGRKFGF+ VP LG  P+ R+ 
Sbjct: 189 SSIFLTNQSLPISM---SQHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRIL 248

Query: 242 --KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGF 301
             K +     + S LA +HN+     + ++  Q+ GFK++  D +  L  R+ +P+K+GF
Sbjct: 249 QPKNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGF 308

Query: 302 KIVDTACCGSDEFRGFFNCGRKKGAIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSG 361
           K  + ACCG+ ++RG F+CG K+    Y LCEN +DY+F+DS H T+ T+ Q A  +W+G
Sbjct: 309 KEGEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNG 368

Query: 362 ----DAHIVKPYNFKQLFQ 373
               D+ +V PYN   LFQ
Sbjct: 369 GHMSDSLVVGPYNINNLFQ 383

BLAST of CmoCh20G011450 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 303.9 bits (777), Expect = 1.8e-82
Identity = 169/369 (45.80%), Postives = 221/369 (59.89%), Query Frame = 0

Query: 10  FFFFFILLGIAAAGSGFPGIQLSDIPSSENRSGFFIFGDSYVDAGNNNYINTTDDFLANF 69
           FF + I+L I +         + +     N++  F+FGDS  DAGNNNYINT   F +N 
Sbjct: 9   FFVYTIILSIGSINC------IDNNNLVTNQAALFVFGDSLFDAGNNNYINTVSSFRSNI 68

Query: 70  PPYGETFFTTPTGRFSDGRIIPDFLAEYANLPLIQPYLDPK--NNLYLNGVNFASGGGGA 129
            PYG+T F  PTGR SDG        E A LP I P L P   NN +  GV+FAS G GA
Sbjct: 69  WPYGQTNFKFPTGRLSDG-------PEKAWLPSIPPNLQPNNGNNQFTYGVSFASAGAGA 128

Query: 130 LAGSHQGQAIGLQTQMKFFKRVKNSLTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFDIS 189
           LA S  G  I L TQ+  FK V+ SL   LG+A T+  FS +V+LF+ G NDY  PF  +
Sbjct: 129 LAESFLGMVINLGTQLNNFKDVEKSLRSELGDAETKRVFSRAVYLFHIGANDYFYPFSAN 188

Query: 190 YDIFKTLDAQEQFADMVIGNITIALKEVYKCGGRKFGFMTVPPLGYTPSSRLK---KNAQ 249
              FK+ +++E+F D VIGNIT  ++EVYK GGRKFGF+ V P   +P+S ++   K   
Sbjct: 189 SSTFKS-NSKEKFVDFVIGNITFVIEEVYKMGGRKFGFLNVGPYECSPNSLIRDRTKIGS 248

Query: 250 FFDETSSLARVHNKLFLMAIQRLATQLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTAC 309
            F   + L  +HNK F   ++RL  QL GF+YA  D HT L +RI +P+KYGFK    AC
Sbjct: 249 CFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYALHDYHTSLSERINSPSKYGFKEGKKAC 308

Query: 310 CGSDEFRGFFNCGRKKG-AIPYTLCENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVK 369
           CGS   RG   CG + G +  Y LCENV DY+F+DS H TEK   Q+A+ +W+G  ++ +
Sbjct: 309 CGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFYDSSHLTEKAHRQIAELIWNGPPNVTR 363

Query: 370 PYNFKQLFQ 373
           PYN K LF+
Sbjct: 369 PYNLKALFE 363

BLAST of CmoCh20G011450 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 296.6 bits (758), Expect = 2.9e-80
Identity = 155/325 (47.69%), Postives = 202/325 (62.15%), Query Frame = 0

Query: 39  NRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYA 98
           N+S  F+FGDS  DAGNNNYI+T   F +N+ PYG+T F  PTGR SDGR IPDF+AEYA
Sbjct: 36  NQSALFVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYA 95

Query: 99  NLPLIQPYLDPKN--NLYLNGVNFASGGGGALAGSHQGQAIGLQTQMKFFKRVKNSLTKR 158
            LPLI  YL P N  N +  GV+FAS G GAL G+  G  I L++Q+  FK+V+  L   
Sbjct: 96  WLPLIPAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRST 155

Query: 159 LGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIALKEVY 218
           LG A+ +   S +V+LF+ G NDY  PF  +  IF++   QE + D V+GN T  +KEVY
Sbjct: 156 LGEAQGKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVY 215

Query: 219 KCGGRKFGFMTVPPLGYTPSSRL---KKNAQFFDETSSLARVHNKLFLMAIQRLATQLDG 278
           K GGRKFGF+ +      P+S +    K    F   + L  +HN+     ++RL  +L G
Sbjct: 216 KIGGRKFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSG 275

Query: 279 FKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKG-AIPYTLCENVQ 338
           FKYA  D HT L  R+ NP+KYGFK    ACCG+   RG   CG + G +  Y LCE V 
Sbjct: 276 FKYALHDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVT 335

Query: 339 DYMFFDSYHPTEKTFEQLAKEMWSG 358
           DY+FFD +H TEK  +Q+A+ +WSG
Sbjct: 336 DYLFFDHFHLTEKAHQQIAELIWSG 359

BLAST of CmoCh20G011450 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 294.3 bits (752), Expect = 1.4e-79
Identity = 155/344 (45.06%), Postives = 207/344 (60.17%), Query Frame = 0

Query: 39  NRSGFFIFGDSYVDAGNNNYINTTDDFLANFPPYGETFFTTPTGRFSDGRIIPDFLAEYA 98
           N++  F FGDS  +AGNNNY ++   F +NF PYG+T F  PTGR SDGRI+ DF+AEYA
Sbjct: 34  NQAALFAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYA 93

Query: 99  NLPLIQPYLDP--KNNLYLNGVNFASGGGGALAGSHQGQAIGLQ----TQMKFFKRVKNS 158
            LPLI P L P   N+    G+NFA+   G  AG+  G    L     TQ+  FK V+ +
Sbjct: 94  WLPLIPPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKT 153

Query: 159 LTKRLGNARTQSFFSNSVFLFNFGGNDYLNPFDISYDIFKTLDAQEQFADMVIGNITIAL 218
           L   LG+A  +   S +V+LF+ G NDY  PF  +   F     +E+F D VIGN T  +
Sbjct: 154 LRSNLGDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN-TTKERFIDFVIGNTTTVI 213

Query: 219 KEVYKCGGRKFGFMTVPPLGYTPSSRL---KKNAQFFDETSSLARVHNKLFLMAIQRLAT 278
           +E+YK G RKFGF+++ P G TPS+ +    K    F+  + L  +HN+ F   ++RL  
Sbjct: 214 EELYKLGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTELINLHNQEFPKVLRRLER 273

Query: 279 QLDGFKYAFADTHTLLLQRILNPTKYGFKIVDTACCGSDEFRGFFNCGRKKG-AIPYTLC 338
           +L GFKYA  D HT L QRI NP++YGFK  + ACCGS   RG   CG + G +  Y LC
Sbjct: 274 RLSGFKYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLC 333

Query: 339 ENVQDYMFFDSYHPTEKTFEQLAKEMWSGDAHIVKPYNFKQLFQ 373
           EN  DY+FFD  H TE   +Q+A+ +WSG  ++  PYN K LF+
Sbjct: 334 ENADDYVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLFR 376