CmoCh20G008540 (gene) Cucurbita moschata (Rifu) v1

BLAST of CmoCh20G008540 vs. ExPASy Swiss-Prot
Match: Q54NX0 (pre-rRNA 2'-O-ribose RNA methyltransferase OS=Dictyostelium discoideum OX=44689 GN=fsjC PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 5.9e-123
Identity = 334/863 (38.70%), Postives = 496/863 (57.47%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K   K RLDK+Y +AKE GYRSRA++KL QL+ KYNFL ++ A LDLCAAPGGWMQVA +
Sbjct: 6   KKLAKGRLDKFYYMAKEQGYRSRAAFKLIQLNKKYNFLGTAKACLDLCAAPGGWMQVASK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGSPN 122
            +PV SL+VGVDLVPI  VR  I + +DIT  +C+  +KK +        D+ LHDG+PN
Sbjct: 66  YMPVQSLIVGVDLVPIRQVRNCIGLTEDITTQKCRTEIKKALK---TWKVDVCLHDGAPN 125

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           +G +W Q+A  Q  L + ++KLAT+ L   G FVTKVFR  DY+S+++   +LF+KVE  
Sbjct: 126 MGTSWVQDAYQQAELTLHALKLATEFLTTGGWFVTKVFRGSDYNSLIWVFNKLFKKVEST 185

Query: 183 KPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRHRD 242
           KP +SR+ASAEI+V+   +  P +IDP+LLD K +F+   E   K VDVL   K+K +R 
Sbjct: 186 KPPSSRNASAEIFVVCQGFLNPKRIDPKLLDPKFVFKEIQEV--KKVDVL-SEKKKVNRA 245

Query: 243 GYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALCDD 302
           GYEDG   L K    S+++ S+  L+ L       FD+ A  + + H+LTT E+K L  D
Sbjct: 246 GYEDGVTVLYKKGFISDFVNSNEHLQDLANFNAFEFDE-AAKIFEQHELTTPEIKELVKD 305

Query: 303 LRVLGKQDFKHLLKWR----LHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEEL 362
           L+VL K DF+ ++KW+     +  K  +P+++ T    +  E   ++ E++ L  EM+E 
Sbjct: 306 LKVLNKNDFQKIIKWKKAMAAYKEKLDNPDEEETEKPEEKKELTAEEMEEN-LQEEMKEY 365

Query: 363 TYAIERKKKREKKLL--AKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRV 422
              +E+KK++EKK     KR+ + K    M +  D +EE   D +L+S+   KGK++   
Sbjct: 366 LALVEKKKRKEKKRQNELKRKHQRKIELTMHIPGDKIEE-TTDGDLYSM---KGKDEFDE 425

Query: 423 VDSTDY-DVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVA 482
               D+ D+ + E    ++D  +++D+   +  ID        DE++E+ LD+ Y+ +  
Sbjct: 426 DIVADHSDISSDEFDSDDSDDDDDDDNNGDSKLIDD-------DEYLEQQLDEQYKLY-- 485

Query: 483 RKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVD--EDRNPLMVSLDDG 542
                 +QR R K A  DD + +++D+ G DG+  D   DE  V+  E+ NPL+V  +  
Sbjct: 486 ------QQRIRKKAAKLDDVK-VKKDKIGQDGYNED---DEEFVEEQEESNPLLVG-NKR 545

Query: 543 GEPTQEEIANKWFGQDIF------------AEAAEDGDLKELDSEDDMQVD-------GP 602
            EP  + +++ +F  ++F            +E  +DGD  + D E++  +D        P
Sbjct: 546 KEPDAQAVSSLFFDNELFGGVEYRNPGDSESEPEQDGD-DDQDDENNKPIDISKLKKQKP 605

Query: 603 KEKTAVTKVAK-SNISKNAREKSKISTNA----KDSIKADD-------GFEVVPAPATDS 662
           +    +TK  K +N ++  ++KSK   N     KD    DD       GFE VP      
Sbjct: 606 QAAQPITKKQKTTNSAEFGKQKSKYQKNPTLDDKDDQDDDDDKGNSIKGFEEVPV----- 665

Query: 663 SNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILDDSYNKYMFDDTGLPKWFLDEEK 722
             +   E   DE+ D K +  A  + ++RKK R+ ++DDS+NKY F+DTGLP WF D+E 
Sbjct: 666 QEEVEYESDSDEDIDDKIKTKALGEFLIRKKSRQDLIDDSFNKYAFNDTGLPNWFTDDEN 725

Query: 723 RHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKARKKRVAMKKLEKVRKKANIISDQ 782
           RH +   P+TKE V  +R + KEID RP KK+AEAKARKK    KK+EK R KA+ I D 
Sbjct: 726 RHNKAQTPLTKEMVDEIRRKIKEIDDRPIKKIAEAKARKKYRLGKKMEKTRDKASSIVDN 785

Query: 783 ADISDRSKSKMIDQLYKKAVPQ--RPKKEFVVAKKGVQVRVGKGK-TLVDRRMKKD--AR 821
            ++S+R KSK I++LY     +  +PKK  ++AKK      G GK  +VD+RMKKD  A+
Sbjct: 786 PEMSNREKSKAIEKLYSGTDKKNMKPKKIIMIAKKSKTAGGGTGKYKIVDKRMKKDLRAQ 830

BLAST of CmoCh20G008540 vs. ExPASy Swiss-Prot
Match: O42832 (AdoMet-dependent rRNA methyltransferase spb1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=spb1 PE=1 SV=2)

HSP 1 Score: 423.7 bits (1088), Expect = 4.8e-117
Identity = 319/831 (38.39%), Postives = 480/831 (57.76%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K   K RLDK+Y+LAKE GYRSRA++KL QL+ KY+FL  +  ++DLCAAPGGW+QVA +
Sbjct: 6   KKTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCAAPGGWLQVASK 65

Query: 63  RVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGSPN 122
               GSL+VGVDL PI P+       +DIT  +C+++L+  +        D++LHDG+PN
Sbjct: 66  TCKPGSLIVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKA---DVVLHDGAPN 125

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A  Q  LV+ S+KLA + L   G FVTKVFRS+DY+++L+  KQLF KVE  
Sbjct: 126 VGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTKVFRSRDYNNLLWVFKQLFNKVEAT 185

Query: 183 KPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKV-VDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   Y AP K+DPR  D + +F+   E    V   V    K+KR R
Sbjct: 186 KPPSSRNVSAEIFVVCRGYKAPKKLDPRFTDPRTVFEEVQEPVTNVDAKVFHPEKRKRSR 245

Query: 243 DGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITF--DDPACLLIKNHDLTTEEVKAL 302
           +GY D D TL K   AS ++ ++ P++ILGT   I F  DD  C  + N D+TTEE+   
Sbjct: 246 EGYADDDYTLHKTVLASEFVTANDPIQILGTSAEIVFPKDDEECQRLYNLDVTTEEILLC 305

Query: 303 CDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELT 362
           C DL+VLGK++F+ +L+WRL IR  +   +K         E   + +E+++L  E+++L+
Sbjct: 306 CSDLQVLGKKEFRDILRWRLKIRDEMGIGKKVEDEQKTVVEEIPEMDEEERLDQELQDLS 365

Query: 363 YAIERKKKREKKLLAKRRAKDKARKAMG----MQLDVMEEGYIDHELFSLSNIKGKNDLR 422
            A   K KRE++   +R+ ++  R  MG    M + +  E   +  LF L+  + K+ L+
Sbjct: 366 EAERVKLKRERRKANQRKQREIVRMQMGMLAPMDIGLEHEAMGEDSLFGLATAE-KHGLK 425

Query: 423 VVDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVA 482
                  +++NG L   E+   +EE    +  + DSD+ER R    +E  LD  Y  +  
Sbjct: 426 -------ELENGTLPVTES--VDEEVSTDNEVEYDSDDERDR----LEADLDSMYSDYTK 485

Query: 483 RKEGSAKQRKRAKKAYSDDAELLEEDENGGD-GFQSDYD--SDENLVDEDRNPLMVSLDD 542
           RK   +  + R KKA  D   L +E+ NG D G +SD    ++ N    D++ L  SL D
Sbjct: 486 RK-AESDVKYRVKKARGD---LDDEEWNGIDNGTESDDSQIAETNFATPDKDRLTTSLLD 545

Query: 543 GGEPTQEEIANK---WFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNIS 602
            G  T++ ++ K   +F QDIF +  ED D        D+++     + A+ K      S
Sbjct: 546 KGS-TKDGLSRKARMFFDQDIF-DGIEDADA-------DVEIMS-MNRAAIKKREAELAS 605

Query: 603 KNAREKSKISTNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTK---AEILACA 662
           +N  + SK       S  ++D  EVVP  +    +D  + +S+++E + +   AE +  A
Sbjct: 606 QNNDDGSK----GDQSEDSNDHIEVVPVASAHDEDDDWNSDSDNDENNVEIVTAEAMTLA 665

Query: 663 KKMV-RKKQREQILDDSYNKYMFDD-TGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFK 722
           + +  R+K +  ++D+ YN++ F    GLP WFLDEE    +P KPITKE V A+R + K
Sbjct: 666 QDIASRRKSKADLIDEGYNRWSFQSKEGLPDWFLDEETTVNKPNKPITKEAVLALREKMK 725

Query: 723 EIDARPAKKVAEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQ 782
            ++ARP KKV EA+ RKK   +K+L++V KKA  IS+  D+++  K+K I +L  +A   
Sbjct: 726 ALNARPIKKVLEAQGRKKMRTIKRLQRVAKKAEGISESGDMTESEKAKEISRLVSRATKS 785

Query: 783 RPKKE--FVVAK---KGVQVRVG--KGK-TLVDRRMKKDAR-KHGMSKQGK 807
           +PK +   VVAK   KG++ R    KGK  +VD RMKKD R +  ++K+G+
Sbjct: 786 KPKAKPTLVVAKGPNKGLKSRPKGVKGKYKMVDSRMKKDLRAQKRLAKKGR 801

BLAST of CmoCh20G008540 vs. ExPASy Swiss-Prot
Match: Q4WVH3 (AdoMet-dependent rRNA methyltransferase spb1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=spb1 PE=3 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 2.5e-113
Identity = 307/821 (37.39%), Postives = 457/821 (55.66%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  S  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLIQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGSPN 122
            +P  S+++GVDL PI P+   I  + DIT  +C+A +++ +        D +LHDG+PN
Sbjct: 65  CMPTQSIIIGVDLAPIKPIPRVITFQSDITTEKCRATIRQHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A SQ  LV+ S+KLAT+ L   G FVTKVFRS+DY+ +L+  KQLF  VE  
Sbjct: 125 VGTAWVQDAFSQAELVLQSMKLATEFLVEGGTFVTKVFRSKDYNPLLWVFKQLFTSVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVES-QRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   + AP +IDP+ LD KH+F    +S       V    K+KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGFKAPKRIDPKFLDPKHVFAELTDSTPNNEARVFNPEKKKRKR 244

Query: 243 DGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFD-----DPACLLIKNHDLTTEEV 302
           +GYE+GD T  K    + +I +  P+ ILGT   ++F+     D A   +   + TT+E+
Sbjct: 245 EGYEEGDYTQFKEIPVTEFINTTDPIAILGTYNKLSFEQSPGGDLALATLNRLEETTDEI 304

Query: 303 KALCDDLRVLGKQDFKHLLKWRLHIRKAL---------SPNQKATLTSVKDAENEVKQNE 362
           +  C+DL++LGK++F+ LL+WRL +R+             ++   +  V   + E+   E
Sbjct: 305 RTCCEDLKILGKKEFRSLLRWRLKVREKFGLVVKKGQAKADEPEEVAEVAPMDEELAIQE 364

Query: 363 DDKLLNEMEELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSN 422
           + + L E E      ER+K+ EKK     R +      M + ++ +  G  D   FSL  
Sbjct: 365 ELQRLQEKESAKRKKERRKENEKKRKEIIRMQMHMTTPMDIGMEQLGPGG-DDATFSLKR 424

Query: 423 IKGKNDLRVVDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLD 482
           ++      V+ S       G+L E E+D  +E+D   S  D +SD+E     + +E  LD
Sbjct: 425 VERDGARDVIAS-------GKLAEIESD--SEDDQTESDYD-ESDDE----GDRLERELD 484

Query: 483 QAYESFVARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGF-QSDYDSDENLVDEDRNPL 542
             YE +  R+E     + RAKKA  D     E +E   DGF  SD + DE   ++  +  
Sbjct: 485 SLYEQYQERRE-DRDSKVRAKKARKD----YEAEE--WDGFSDSDKEDDEESEEDGASQA 544

Query: 543 MVSLDDGGEPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKS 602
           +V        T    A  +F QDIF    +  D+++ DS  +MQ D    K        S
Sbjct: 545 VVKPAPPNSGTLSSKAAMFFDQDIFQGLGDVDDVEDEDSAIEMQEDDKSAKKG------S 604

Query: 603 NISKNAREKSKISTNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACA 662
            + K A +++K    A +     D          +  +D   +  + +     AE +A A
Sbjct: 605 ALEKKAPKEAKKKAQAPEDFSDSD---------PEEPDDPRKKNGQLDIDIITAEAMALA 664

Query: 663 KKMVR-KKQREQILDDSYNKYMFDDT-GLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFK 722
           ++M   +K+ + I+DD +N+Y F D  GLP+WFLD+E +H +P +PITK   AA++ + +
Sbjct: 665 QQMATGEKKSQDIIDDGFNRYTFRDVDGLPEWFLDDENKHSKPQRPITKAAAAAIKEKLR 724

Query: 723 EIDARPAKKVAEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQ 782
            I+ARP KKV EAK RKK  A ++LEK+RKK+ +++D   +S+R KS+ I +L  KAV +
Sbjct: 725 AINARPIKKVMEAKGRKKMKAAQRLEKLRKKSALLADDEALSERDKSQAIAKLMSKAVKK 784

Query: 783 RPKKE--FVVAK---KGV--QVRVGKGK-TLVDRRMKKDAR 798
           +PK++   VVA+   +G+  + R  KGK  +VD RMKKD R
Sbjct: 785 KPKQQVKLVVARGANRGISGRPRGVKGKYKIVDSRMKKDIR 785

BLAST of CmoCh20G008540 vs. ExPASy Swiss-Prot
Match: Q52C47 (AdoMet-dependent rRNA methyltransferase SPB1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=SPB1 PE=3 SV=2)

HSP 1 Score: 399.1 bits (1024), Expect = 1.3e-109
Identity = 312/865 (36.07%), Postives = 484/865 (55.95%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+Y+LAKE GYR+RA++KL QL+ KY FL  S  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYKLAKEKGYRARAAFKLIQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGSPN 122
            +PV SL+VGVDL PI P+   I  + DIT  +C+A +++ +        D +LHDG+PN
Sbjct: 65  VMPVSSLIVGVDLAPIKPIPKVITFQSDITTEKCRATIRQHLKTWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q++ +Q  L + ++KLAT+ L   G FVTKVFRS+DY+S+L+   QLF+KVE  
Sbjct: 125 VGTAWVQDSFNQAELTLQAMKLATEFLVEGGTFVTKVFRSKDYNSMLWVFNQLFKKVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVE-SQRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   + AP +IDP+ LD + +F    + +      V +   +KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGFKAPKRIDPKFLDPRAVFAELADPTPNNEAKVYKPEIKKRKR 244

Query: 243 DGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFD-----DPACLLIKNHDLTTEEV 302
           DGYE+GD T  K   A  +I S  P+ ILG+   ++ +     D A  +++    TT+E+
Sbjct: 245 DGYEEGDYTQYKELPAYEFIQSTDPIAILGSTNRLSLEQSKNGDVALAVLEKLPETTDEI 304

Query: 303 KALCDDLRVLGKQDFKHLLKWRLHIRKALS-PNQK---------ATLTSVKDAENEVKQN 362
           +  C DL+VLG+++FK LLKWRL +R+ L  P +K         A + + ++       +
Sbjct: 305 RTCCADLKVLGRKEFKLLLKWRLAVREKLGFPTKKSVKKEEEAAAAVAAAEEVAKIESMD 364

Query: 363 EDDKLLNEMEELTYAIERKKKREKKLLAKRRAKDKARKAMGM--QLDV-MEEGYIDHE-- 422
           E+ ++ +E+E+L      KKKRE++   +R+ KD  R  M M   +D+ +E+   + E  
Sbjct: 365 EEMRIQHELEKLKERNSTKKKRERRKENERKQKDIVRMQMHMVAPMDIGVEQAGPEGEDA 424

Query: 423 LFSLSNIKGKNDLRVVDSTDYDVDNGELGEHENDVTNEEDHG---SSASDIDSDEERRRY 482
           +F+L  ++  + +R +      V +      E D   + D G   S  +D +SDEE  R 
Sbjct: 425 MFALRAVEKGDVMRRLAKGKMVVAS------EADAKKDRDSGIGSSGETDDESDEELDR- 484

Query: 483 DEHMEELLDQAYESFVARKEGS-AKQR-KRAKKAYSDDAELLEEDENGGDGFQSDYDSDE 542
              +E  LD  Y+ F  RK  S AK R K+A++A + D +  EE E   D  ++D  SD+
Sbjct: 485 ---LETELDDMYDQFRERKAASDAKYRAKKARQARNGDGD--EEWEGVSDNEKADEISDD 544

Query: 543 NLVDED-------------RNPLMVSLDDGGEPTQ--EEIANKWFGQDIFAEAAEDGDLK 602
           + ++E+             R  L+  LD          + A  +F QDIF E   DGD+ 
Sbjct: 545 SELEEESSGDSDDEDDTAPRKSLLTDLDTTPSDNSGLSKRARAFFNQDIFKEL--DGDMD 604

Query: 603 E-LDSE---------DDMQVDGPKEKTAVTKVAKSNISKNAREKSKISTNAKDSIKADD- 662
           E +D E         +D  ++    K    K  +    K A +K+K +      +K DD 
Sbjct: 605 EPMDEELRAALAGEDEDADMEDTVSKADSKKTKEKTADKKAAKKAKKAAQKAQQVKDDDS 664

Query: 663 ------GFEVVPAPATD--SSNDSSSEESEDEEPDTKAEILACAKKMVR-KKQREQILDD 722
                 GFEVV +   D     D  +++   +     AE +  A ++   +K    ++DD
Sbjct: 665 DDESDGGFEVVKSGKEDDWEDEDKRTKDGRLDIDIITAEAMTLAHQLATGQKSSHDVIDD 724

Query: 723 SYNKYMFDD-TGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 782
            +NK+ F D  GLP+WFLD+E +H +P KPITK   AA++ + +  +ARP KKV EAK R
Sbjct: 725 GFNKHAFKDREGLPEWFLDDETKHDKPQKPITKAAAAAIKEKMRAFNARPIKKVREAKGR 784

Query: 783 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKK--EFVVAK---K 798
           KK  A ++LEK++KK++++ ++  ++++ K++ I +L +KA  ++PK+  + VVAK   +
Sbjct: 785 KKMKAAQRLEKLKKKSDLLVNEEGMTEKEKAESIAKLLRKATKKKPKQAVKVVVAKGANR 844

BLAST of CmoCh20G008540 vs. ExPASy Swiss-Prot
Match: Q5BH88 (AdoMet-dependent rRNA methyltransferase spb1 OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=spb1 PE=3 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.5e-107
Identity = 306/827 (37.00%), Postives = 451/827 (54.53%), Query Frame = 0

Query: 3   KVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVE 62
           K  GK RLDK+YRLAKE GYR+RA++KL QL+ KY FL  S  +LDLCAAPG W QVA E
Sbjct: 5   KKHGKGRLDKWYRLAKEKGYRARAAFKLVQLNKKYGFLEKSKVLLDLCAAPGSWCQVAAE 64

Query: 63  RVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGSPN 122
            +P  SL+VGVDL PI P+   I  +QDIT  +C+A ++  +        D +LHDG+PN
Sbjct: 65  CMPAQSLIVGVDLAPIKPIPRVITFQQDITTEKCRATIRSHLKHWKA---DTVLHDGAPN 124

Query: 123 VGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVEVD 182
           VG AW Q+A SQ  LV++S+KLAT+ L   G FVTKVFRS+DY+ +L+  KQLF  VE  
Sbjct: 125 VGTAWVQDAFSQAELVLESLKLATEFLVEGGTFVTKVFRSKDYNPLLWVFKQLFMSVEAT 184

Query: 183 KPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVE-SQRKVVDVLRGTKQKRHR 242
           KP +SR+ SAEI+V+   Y AP +IDP+ LD KH+F    + +      V    K+KR R
Sbjct: 185 KPPSSRNVSAEIFVVCRGYKAPKRIDPKFLDSKHVFAELADPTPNNEAKVFNPEKKKRKR 244

Query: 243 DGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFD-----DPACLLIKNHDLTTEEV 302
           +GYE+GD T  K    + +I +  P+ ILG+   ++F      D A   +     TT+E+
Sbjct: 245 EGYEEGDWTQFKEIPVTEFINTTDPIAILGSCNKLSFQQQPGGDLALATLDRLPETTDEI 304

Query: 303 KALCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEME 362
           +  C+DL+VLGK++F++LL+WRL +R+      K       +AE   +    D  L   E
Sbjct: 305 RNCCEDLKVLGKKEFRNLLRWRLKVREKFGLVVKKGQAKDDEAEEVAEIAPMDDELAIQE 364

Query: 363 ELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRV 422
           EL    E++  R KK   K   + K ++ + MQ+ +     I  E            L+ 
Sbjct: 365 ELLRLKEKESARSKKERRKENER-KRKEIVRMQMHMTTPMDIGMEQLGPGGEDATFSLKR 424

Query: 423 VDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVAR 482
            +           G+    + +E +   S ++ D  ++     + +E  LD  YE +  R
Sbjct: 425 AEKAGAAAAMAS-GKELAVIESESEESESETEYDDSDDE---GDRLERELDSLYEQYQER 484

Query: 483 KEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEP 542
           KE     + RAKKA  D     E DE   +GF SD D + +  +ED     V +     P
Sbjct: 485 KE-DKDSKLRAKKARKD----YEADE--WEGF-SDSDKEGSDDEEDEAKPNVPV----VP 544

Query: 543 TQEEIANK---WFGQDIF--AEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKN 602
           T   ++N    +F QDIF   +  ED +  E +  ++M V   +E+    +  +   S+ 
Sbjct: 545 TNGALSNNAALFFDQDIFQGLDDVEDEE-DEAEGTNNMTVANEREEQQEEEEEEQPKSEA 604

Query: 603 AREKSKISTNAKDSIKADDGFEVVPAPATDSSNDSSSEESED-EEPDTK----------A 662
           A EK K               +V     +  S DSS +E ED +EP  K          A
Sbjct: 605 APEKKK---------------KVKETANSKPSEDSSDDEYEDTDEPRKKNGQLDIDIITA 664

Query: 663 EILACAKKMVR-KKQREQILDDSYNKYMFDDT-GLPKWFLDEEKRHRQPIKPITKEEVAA 722
           E +A A++M   +K+ + + DD +N+Y F D  GLP+WFLD+E +H +P +PITK   AA
Sbjct: 665 EAMALAQQMATGEKKSQDVFDDGFNRYAFRDVDGLPEWFLDDENKHSKPNRPITKAAAAA 724

Query: 723 MRAQFKEIDARPAKKVAEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLY 782
           ++ +++ I+ARP KKV EAK RKK  A +++EK+RKK+ +++D   +S+R K++ I ++ 
Sbjct: 725 IQEKWRAINARPIKKVMEAKGRKKFKAAQRIEKLRKKSALLADDETMSERDKAQAIARMM 784

Query: 783 KKAVPQRPKK--EFVVAKKGVQVRVG-----KGK-TLVDRRMKKDAR 798
            +A  ++PK+  + VVAK G +   G     KGK  +VD RMKKD R
Sbjct: 785 SRAAKKKPKQNVKLVVAKGGNRGISGRPKGVKGKYKIVDARMKKDVR 795

BLAST of CmoCh20G008540 vs. ExPASy TrEMBL
Match: A0A6J1FTB8 (Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111448289 PE=3 SV=1)

HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 836/836 (100.00%), Postives = 836/836 (100.00%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS
Sbjct: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST
Sbjct: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 837
           VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 836

BLAST of CmoCh20G008540 vs. ExPASy TrEMBL
Match: A0A6J1L3E5 (Putative rRNA methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111499543 PE=3 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 758/837 (90.56%), Postives = 795/837 (94.98%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDIT+PECKARLKKIMNEKGCAAFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGALAIEQDITRPECKARLKKIMNEKGCAAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKG FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGD+TLRK SSASN+IWSDSPLEILGTVT ITF+DPACLLIK+H+LTTEEVK LC
Sbjct: 241 RDGYEDGDMTLRKESSASNFIWSDSPLEILGTVTTITFNDPACLLIKDHELTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDL VLGKQDFKHLLKWRLHIRKALSP QK T TSV DAENEVKQ+EDDKLLNEMEELTY
Sbjct: 301 DDLGVLGKQDFKHLLKWRLHIRKALSPKQKVTSTSVNDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           A+ERKKKREKKLLAKR+AKDKARKA+GMQLDVME+GYIDHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AMERKKKREKKLLAKRKAKDKARKALGMQLDVMEDGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           +YD +N E+GE E  VT EE+HGSS SD+DSDEERRRYD H+EELLDQAY+SFVARKEGS
Sbjct: 421 EYDDENDEMGEQEIGVTKEENHGSSDSDLDSDEERRRYDAHIEELLDQAYDSFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
            KQRKRAKKAYSDDAELL+EDENGGDG  SDYDSDENLVDED+NPLMVSLD GG PT+EE
Sbjct: 481 TKQRKRAKKAYSDDAELLKEDENGGDG-PSDYDSDENLVDEDKNPLMVSLDYGGVPTEEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKEL-DSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIS 600
           +ANKWF QDIFAEA EDGDLKEL DSEDDMQVD PKEK AV+K AK NISK  REK K S
Sbjct: 541 VANKWFSQDIFAEAVEDGDLKELVDSEDDMQVDRPKEKAAVSKEAKPNISKKEREKLKTS 600

Query: 601 TNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQIL 660
           TNA+++ K DDGFEVVPAPATDSS+DSSS+ESEDE+PDTKAEILACAKKM+RKKQREQIL
Sbjct: 601 TNARETDKGDDGFEVVPAPATDSSDDSSSDESEDEDPDTKAEILACAKKMLRKKQREQIL 660

Query: 661 DDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKA 720
           DD+YNKYMFDDTGLP WFLDEE+RHR+PIKPITKEEVAAM+AQFKEIDARPAKKVAEAKA
Sbjct: 661 DDAYNKYMFDDTGLPSWFLDEERRHRRPIKPITKEEVAAMKAQFKEIDARPAKKVAEAKA 720

Query: 721 RKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQV 780
           RKKRVAMKKLEKVRKKAN ISDQADISDRSK KMIDQLYKKAVP+RPKKEFVVAKKGVQV
Sbjct: 721 RKKRVAMKKLEKVRKKANSISDQADISDRSKRKMIDQLYKKAVPERPKKEFVVAKKGVQV 780

Query: 781 RVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 837
           RVGKGK LVDRRMKKD RKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK
Sbjct: 781 RVGKGKVLVDRRMKKDMRKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 836

BLAST of CmoCh20G008540 vs. ExPASy TrEMBL
Match: A0A6J1G7D4 (Putative rRNA methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111451531 PE=3 SV=1)

HSP 1 Score: 1417.1 bits (3667), Expect = 0.0e+00
Identity = 760/838 (90.69%), Postives = 793/838 (94.63%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDIT+PECKARLKKIMNEKGCAAFDL+LHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGALAIEQDITRPECKARLKKIMNEKGCAAFDLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKG FVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGTFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGD+TLRK SSASN+IWSDSPLEILGTVT ITF+DPACL IK+H+LTTEEVK LC
Sbjct: 241 RDGYEDGDMTLRKESSASNFIWSDSPLEILGTVTTITFNDPACLPIKDHELTTEEVKTLC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDL VLGKQDFKHLLKWRLHIRKALSP QK T TSV DAENEVKQ+EDDKLLNEMEELTY
Sbjct: 301 DDLGVLGKQDFKHLLKWRLHIRKALSPKQKVTSTSVNDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           A+ERKKKREKKLLAKR+AKDKARKA+GMQLDVME+GYIDHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AMERKKKREKKLLAKRKAKDKARKALGMQLDVMEDGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           +YD DN E+GE EN VT EE+HGSS SDIDSDEERRRYD HMEELLDQAYESFVARKEGS
Sbjct: 421 EYDDDNDEMGEQENGVTKEENHGSSDSDIDSDEERRRYDAHMEELLDQAYESFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
            KQRKRAKKAYSDDAELL+EDE+GGDG  SDYDSDENLVDED+NPLMVSLDDGG PT+EE
Sbjct: 481 TKQRKRAKKAYSDDAELLKEDESGGDG-PSDYDSDENLVDEDKNPLMVSLDDGGVPTEEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKEL-DSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIS 600
           IANKWF QDIFAEA EDGDLKEL DSEDDMQ+D PKEK AV+K AK NISK  REKSK S
Sbjct: 541 IANKWFSQDIFAEAVEDGDLKELVDSEDDMQIDRPKEKAAVSKEAKPNISKKEREKSKTS 600

Query: 601 TNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQIL 660
           TNA+++ K DDGFEVVPAPATDSS+DSSS+ESEDE+PDTKAEILACAKKM+RKKQREQIL
Sbjct: 601 TNARETDKEDDGFEVVPAPATDSSDDSSSDESEDEDPDTKAEILACAKKMLRKKQREQIL 660

Query: 661 DDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKA 720
           DD+YNKYMFDDTGLP WFLDEE+RHRQPIKPITKEEVAAM+AQFKEIDARPAKKVAEAKA
Sbjct: 661 DDAYNKYMFDDTGLPSWFLDEERRHRQPIKPITKEEVAAMKAQFKEIDARPAKKVAEAKA 720

Query: 721 RKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQV 780
           RKKRVAMKKLEKVRKKAN ISDQADISDRSK KMIDQLYKKAVP+RPKKEFVVAKKGVQV
Sbjct: 721 RKKRVAMKKLEKVRKKANSISDQADISDRSKRKMIDQLYKKAVPERPKKEFVVAKKGVQV 780

Query: 781 RVGKGKTLVDRRMKKDARKHGMSKQGKGSK----KGKNSKAPRGKGGSAKASGKKGRK 834
           RVGKGK LVDRRMKKD RKHGMSKQGKGSK    KGKNSKAPRGKGG AKASGKKGRK
Sbjct: 781 RVGKGKVLVDRRMKKDMRKHGMSKQGKGSKKGKGKGKNSKAPRGKGGPAKASGKKGRK 837

BLAST of CmoCh20G008540 vs. ExPASy TrEMBL
Match: A0A0A0LIR7 (Putative rRNA methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_2G248140 PE=3 SV=1)

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 750/855 (87.72%), Postives = 797/855 (93.22%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+A EQDITKPECKARLKKIM+EKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAFEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAM+QNSLVIDSV+LATQLLAPKG FVTKVFRSQDYSSVLYC+KQLFEKVE
Sbjct: 121 PNVGGAWAQEAMAQNSLVIDSVRLATQLLAPKGTFVTKVFRSQDYSSVLYCMKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLG+RY APAKIDPRLLDVK+LFQGS+E Q+KVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGIRYKAPAKIDPRLLDVKYLFQGSIEPQQKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDG  TLRKVSSASN+IWSDSPLE+LGTVTCI FDDP  L IK+HDLTTEEVKALC
Sbjct: 241 RDGYEDGATTLRKVSSASNFIWSDSPLEVLGTVTCIAFDDPDSLPIKDHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSP+QK T TSVKDAENEVKQ+EDDKLLNEMEEL Y
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPDQKPTSTSVKDAENEVKQDEDDKLLNEMEELAY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           A+ERKKKR KKLLAKR+AKDKARKAMG QLDVMEEGY+DHELFSLSNIKGKNDLR VDST
Sbjct: 361 AMERKKKRAKKLLAKRKAKDKARKAMGTQLDVMEEGYVDHELFSLSNIKGKNDLRAVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           +YD DN ELGEHENDVT +++ GSSASDIDSDEERRRYDEHMEELLDQAYESFV+RKEGS
Sbjct: 421 EYDDDNVELGEHENDVTKDKNRGSSASDIDSDEERRRYDEHMEELLDQAYESFVSRKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AK+RKR K AYSD+AELLEEDENGGDG QSDYDSDEN+VD D+NPLMVSLDDG EPTQEE
Sbjct: 481 AKRRKRVKNAYSDNAELLEEDENGGDGIQSDYDSDENIVDADKNPLMVSLDDGAEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IA+KWF QDIFAEAAE+GDLK LDS+DDM+VDGPKE  AV+K AKSNIS+NA EKSKIST
Sbjct: 541 IASKWFSQDIFAEAAEEGDLKRLDSDDDMEVDGPKETLAVSKKAKSNISQNAGEKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NA++S K DDGFEVVPAPATDSS+ SSSEES+DE+PDT+AEILACAKKM+RKKQREQILD
Sbjct: 601 NARESNKVDDGFEVVPAPATDSSDSSSSEESDDEDPDTRAEILACAKKMLRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDD+GLPKWFLDEE+RHRQPIKPITKEEVAA+RAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDSGLPKWFLDEEQRHRQPIKPITKEEVAAIRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKR+AMKKLEKVRKKAN+ISDQADISDRSK KMIDQLYKKAVPQ+PKKE VVAKKGVQVR
Sbjct: 721 KKRIAMKKLEKVRKKANVISDQADISDRSKRKMIDQLYKKAVPQKPKKELVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSA---------------- 837
           VGKGK LVDRRMKKDARKHGM+KQGKGSKKGKNSKAPR KGG A                
Sbjct: 781 VGKGKVLVDRRMKKDARKHGMNKQGKGSKKGKNSKAPRAKGGPAKDSNTPGGKAGRAGKA 840

BLAST of CmoCh20G008540 vs. ExPASy TrEMBL
Match: A0A1S4E314 (Putative rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103499682 PE=3 SV=1)

HSP 1 Score: 1409.0 bits (3646), Expect = 0.0e+00
Identity = 752/850 (88.47%), Postives = 796/850 (93.65%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIM+EKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSV+LATQLLAPKG FVTKVFRSQDYSSVLYC+KQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVRLATQLLAPKGTFVTKVFRSQDYSSVLYCIKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIY+LG+RY APAKIDPRLLDVKHLFQGSVE Q+KVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYILGIRYKAPAKIDPRLLDVKHLFQGSVEPQQKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDG   LRKVSSASN+IWSDSPLEILGTVTCITFD+P  L IK+HDLTTEEVKALC
Sbjct: 241 RDGYEDGATILRKVSSASNFIWSDSPLEILGTVTCITFDEPDSLPIKDHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSP+QKAT TSVKD ENEVKQ+EDDKLLNEMEEL Y
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPDQKATSTSVKDVENEVKQDEDDKLLNEMEELAY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           A+ERKKKR KKLLAKR+AKDKARKAMG Q+DVMEEGY+DHELFSLSNIKGKNDL+ VDST
Sbjct: 361 AMERKKKRTKKLLAKRKAKDKARKAMGTQIDVMEEGYVDHELFSLSNIKGKNDLKAVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           +YD DNGELGEHENDVT EE+ GSSASDIDSDEERRRYDEHMEELLDQAYESFV+RKEGS
Sbjct: 421 EYDDDNGELGEHENDVTKEENRGSSASDIDSDEERRRYDEHMEELLDQAYESFVSRKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AK+RKR K AYSD+AELLEEDENGGDG QSDYDSDEN+VD D+NPLMVSLDDG EPTQEE
Sbjct: 481 AKRRKRVKNAYSDNAELLEEDENGGDGIQSDYDSDENIVDADKNPLMVSLDDGAEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IA+KWF QDIFAEA E+GDLK LDS+DDM+VDGPKE  AV+K AKS+IS+NA E SKIS+
Sbjct: 541 IASKWFSQDIFAEAVEEGDLKGLDSDDDMEVDGPKETLAVSKKAKSSISQNAGENSKISS 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NA++S + DDGFEVVPAPATDSS+ SSSEES+DE+PDT+AEILACAKKM+RKKQREQILD
Sbjct: 601 NARESNEVDDGFEVVPAPATDSSDSSSSEESDDEDPDTRAEILACAKKMLRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDD GLPKWFLDEE+RHRQPIKP+TKEEVAA+RAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDAGLPKWFLDEERRHRQPIKPVTKEEVAAIRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMKKLEKVRKKAN+ISDQADISDRSK KMIDQLYKKAVPQRPKKE VVAKKGVQVR
Sbjct: 721 KKRVAMKKLEKVRKKANVISDQADISDRSKRKMIDQLYKKAVPQRPKKELVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSA-------------KAS 837
           VGKGK LVDRRMKKDARKHGMSKQGKGSKKGKNSKAPR KGG A             KAS
Sbjct: 781 VGKGKVLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRAKGGPAKDSNTPRGKAGFTKAS 840

BLAST of CmoCh20G008540 vs. NCBI nr
Match: XP_022943552.1 (putative rRNA methyltransferase [Cucurbita moschata])

HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 836/836 (100.00%), Postives = 836/836 (100.00%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS
Sbjct: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST
Sbjct: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 837
           VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 836

BLAST of CmoCh20G008540 vs. NCBI nr
Match: KAG6571106.1 (hypothetical protein SDJN03_30021, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1556.2 bits (4028), Expect = 0.0e+00
Identity = 830/836 (99.28%), Postives = 831/836 (99.40%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYRAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           DY VDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS
Sbjct: 421 DYVVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IANKWFGQDIFA+AAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST
Sbjct: 541 IANKWFGQDIFAKAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NAKDSIKADDGFEVVP PATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD
Sbjct: 601 NAKDSIKADDGFEVVPPPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDDTGLPKWFLDEEKRH QPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHHQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 837
           VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 836

BLAST of CmoCh20G008540 vs. NCBI nr
Match: KAG7010927.1 (spb1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1553.5 bits (4021), Expect = 0.0e+00
Identity = 831/842 (98.69%), Postives = 833/842 (98.93%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEE----- 300
           RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEE     
Sbjct: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVPVII 300

Query: 301 -VKALCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNE 360
            VKALCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQNEDDKLLNE
Sbjct: 301 WVKALCDDLRVLGKQDFKHLLKWRLHIRKALSPNQKATPTSVKDAENEVKQNEDDKLLNE 360

Query: 361 MEELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDL 420
           MEELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDL
Sbjct: 361 MEELTYAIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDL 420

Query: 421 RVVDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFV 480
           RV+DSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFV
Sbjct: 421 RVIDSTDYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFV 480

Query: 481 ARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGG 540
           ARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGG
Sbjct: 481 ARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGG 540

Query: 541 EPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNARE 600
           EPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNARE
Sbjct: 541 EPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNARE 600

Query: 601 KSKISTNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQ 660
           KSKISTNAKDSIKADDGFEVVPAPATDSS+DSSSEESEDEEPDTKAEILACAKKMVRKKQ
Sbjct: 601 KSKISTNAKDSIKADDGFEVVPAPATDSSDDSSSEESEDEEPDTKAEILACAKKMVRKKQ 660

Query: 661 REQILDDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKV 720
           REQILDDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKV
Sbjct: 661 REQILDDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKV 720

Query: 721 AEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAK 780
           AEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAK
Sbjct: 721 AEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAK 780

Query: 781 KGVQVRVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKG 837
           KGVQVRVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKG
Sbjct: 781 KGVQVRVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKG 840

BLAST of CmoCh20G008540 vs. NCBI nr
Match: XP_023512222.1 (adoMet-dependent rRNA methyltransferase spb1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1538.5 bits (3982), Expect = 0.0e+00
Identity = 821/836 (98.21%), Postives = 826/836 (98.80%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           V+KPAASRSASAEIYVLGLRY APAKIDPRLLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VEKPAASRSASAEIYVLGLRYKAPAKIDPRLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGD+TLRKVSSASNYIWSDSPLEILGTVTCITFDDP CLLIKNHDLTTEEVKALC
Sbjct: 241 RDGYEDGDMTLRKVSSASNYIWSDSPLEILGTVTCITFDDPPCLLIKNHDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRLHIRKALSPNQKAT TSVKDAENEVKQNEDDKLLNEMEELTY
Sbjct: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATPTSVKDAENEVKQNEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRV+DST
Sbjct: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVIDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           DYDVDNGELGEHENDVTNEEDH SSASDI SDEERRRYDEHMEELLDQAYESFVARKEGS
Sbjct: 421 DYDVDNGELGEHENDVTNEEDHESSASDIGSDEERRRYDEHMEELLDQAYESFVARKEGS 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AKQRKRAKKAYSDDAELLEEDENGGD FQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE
Sbjct: 481 AKQRKRAKKAYSDDAELLEEDENGGDRFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IANKWFGQDIFAEAAEDGDLKEL SEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST
Sbjct: 541 IANKWFGQDIFAEAAEDGDLKELGSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
           NAKDSIKADD FEVVPAPATDSS+DSSSEESEDEEPDTKAEILACAKKM+RKKQREQ LD
Sbjct: 601 NAKDSIKADDEFEVVPAPATDSSDDSSSEESEDEEPDTKAEILACAKKMLRKKQREQFLD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR
Sbjct: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 837
           VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK
Sbjct: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKASGKKGRKGNK 836

BLAST of CmoCh20G008540 vs. NCBI nr
Match: XP_038901448.1 (adoMet-dependent rRNA methyltransferase spb1 [Benincasa hispida])

HSP 1 Score: 1422.5 bits (3681), Expect = 0.0e+00
Identity = 760/849 (89.52%), Postives = 796/849 (93.76%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQ+A
Sbjct: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQIA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VERVPVGSLVVGVDLVPIAPVRGA+AIEQDITKPECKARLKKIM++KGCAAFDLILHDGS
Sbjct: 61  VERVPVGSLVVGVDLVPIAPVRGAVAIEQDITKPECKARLKKIMSDKGCAAFDLILHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAM+QNSLVIDSVKLATQLLAPKG FVTKVFRSQDYSSVLYCLKQLF KVE
Sbjct: 121 PNVGGAWAQEAMAQNSLVIDSVKLATQLLAPKGTFVTKVFRSQDYSSVLYCLKQLFNKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           VDKPAASRS SAEIY+LG  Y APAKIDP LLDVKHLFQGSVE QRKVVDVLRGTKQKRH
Sbjct: 181 VDKPAASRSTSAEIYILGFSYKAPAKIDPHLLDVKHLFQGSVEPQRKVVDVLRGTKQKRH 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDGD+TLRKVSSASN+IWSDSPLEILGTVTCITFDDPACL IK++DLTTEEVKALC
Sbjct: 241 RDGYEDGDMTLRKVSSASNFIWSDSPLEILGTVTCITFDDPACLPIKDNDLTTEEVKALC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDLRVLGKQDFKHLLKWRL+IRKALSPN KAT T VKDAENEVKQ+EDDKLLNEMEELTY
Sbjct: 301 DDLRVLGKQDFKHLLKWRLNIRKALSPNLKATSTLVKDAENEVKQDEDDKLLNEMEELTY 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
           A+ERKKKR KKLLAKR+AKDKARKAMG QLDVMEEGY+DHELFSLSNIKGKNDLRVVDST
Sbjct: 361 AMERKKKRAKKLLAKRKAKDKARKAMGAQLDVMEEGYVDHELFSLSNIKGKNDLRVVDST 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSASDIDSDEERRRYDEHMEELLDQAYESFVARKEGS 480
           DYD  N E GEHENDVTNEE+HGSSASDIDSDEERRRYDEHMEELLDQAYESF++RKEG+
Sbjct: 421 DYDDGNDEFGEHENDVTNEENHGSSASDIDSDEERRRYDEHMEELLDQAYESFISRKEGT 480

Query: 481 AKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGGEPTQEE 540
           AKQRKRA+KAYSDD E+LEEDENG DG QSDYDSDEN+VD DRNPLMVSLDDG EPTQE+
Sbjct: 481 AKQRKRARKAYSDDVEMLEEDENGVDGVQSDYDSDENIVDADRNPLMVSLDDGAEPTQED 540

Query: 541 IANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNAREKSKIST 600
           IANKWF QDIFAEAAE+GDLK LDSEDD QVDGPKE TAV+K  KSNI KNAREKSKIST
Sbjct: 541 IANKWFSQDIFAEAAEEGDLKGLDSEDDTQVDGPKESTAVSKEGKSNIPKNAREKSKIST 600

Query: 601 NAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAKKMVRKKQREQILD 660
              +S +AD+GFEVVPAPATDSS+ SSSEES+DE+PDTKAEILACAKKM+RKKQREQILD
Sbjct: 601 ---ESNEADNGFEVVPAPATDSSDSSSSEESDDEDPDTKAEILACAKKMLRKKQREQILD 660

Query: 661 DSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEIDARPAKKVAEAKAR 720
           DSYNKYMFDDTGLPKWFLDEE+RHRQPIKP+TKEEVAAMRAQFKEIDARPAKKVAEAKAR
Sbjct: 661 DSYNKYMFDDTGLPKWFLDEERRHRQPIKPVTKEEVAAMRAQFKEIDARPAKKVAEAKAR 720

Query: 721 KKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAVPQRPKKEFVVAKKGVQVR 780
           KKRVAMK+LEKVRKKAN+ISDQADISDRSK KMIDQLYKKAVPQRPKKE VVAKKGVQVR
Sbjct: 721 KKRVAMKRLEKVRKKANVISDQADISDRSKRKMIDQLYKKAVPQRPKKELVVAKKGVQVR 780

Query: 781 VGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKG-------------GSAKAS 837
           VGKGK LVDRRMKKDARKHGMSKQGKGSKKGKNSKAPR KG             G AKAS
Sbjct: 781 VGKGKVLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRAKGGFAKDSKAPRGNAGFAKAS 840

BLAST of CmoCh20G008540 vs. TAIR 10
Match: AT4G25730.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 960.3 bits (2481), Expect = 1.0e-279
Identity = 537/844 (63.63%), Postives = 660/844 (78.20%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGKVKGKHRLDKYYRLAKE G+RSRAS+KL QLD+KY+ L S+HAVLDLCAAPGGWMQVA
Sbjct: 1   MGKVKGKHRLDKYYRLAKERGFRSRASYKLLQLDAKYSLLHSAHAVLDLCAAPGGWMQVA 60

Query: 61  VERVPVGSLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKGCAAFDLILHDGS 120
           VE+VPVGSLV+G+DLVPI PVRG + + QDIT+ ECK+++K++M + G +AF+L+LHDGS
Sbjct: 61  VEKVPVGSLVLGIDLVPILPVRGCVTMTQDITRTECKSKIKQVMEQHGVSAFNLVLHDGS 120

Query: 121 PNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSVLYCLKQLFEKVE 180
           PNVGGAWAQEAMSQN+LVIDSV+LAT+ LA  G  VTKVFRS+DY+SVLYCL +LFEKVE
Sbjct: 121 PNVGGAWAQEAMSQNALVIDSVRLATEFLARNGNLVTKVFRSRDYNSVLYCLGRLFEKVE 180

Query: 181 VDKPAASRSASAEIYVLGLRYTAPAKIDPRLLDVKHLFQGSVESQRKVVDVLRGTKQKRH 240
           V KP ASRSASAE Y++GL+Y APAKIDPRLLD +HLF+ S E  RKVVDVL G+KQKR+
Sbjct: 181 VFKPPASRSASAETYLVGLKYLAPAKIDPRLLDYRHLFKESAEPTRKVVDVLGGSKQKRN 240

Query: 241 RDGYEDGDLTLRKVSSASNYIWSDSPLEILGTVTCITFDDPACLLIKNHDLTTEEVKALC 300
           RDGYEDG+  LR+V+SA+++IWS++PL++LGT T I+FDD A L +K HDLTTEE+K LC
Sbjct: 241 RDGYEDGESILRRVASAADFIWSENPLDVLGTTTSISFDDQASLPLKEHDLTTEEIKILC 300

Query: 301 DDLRVLGKQDFKHLLKWRLHIRKALSPNQKATLTSVKDAENEVKQNEDDKLLNEMEELTY 360
           DDL VLGK DFKH+LKWR+ IRKAL+P +K       D   E ++NEDDKLLNE+EELT 
Sbjct: 301 DDLPVLGKNDFKHILKWRMQIRKALTPEKKEVAKPEPDVGKEDEENEDDKLLNELEELTN 360

Query: 361 AIERKKKREKKLLAKRRAKDKARKAMGMQLDVMEEGYIDHELFSLSNIKGKNDLRVVDST 420
            ++RKKK+ KK+LAKRRAKDKARKA G Q+DV+E+G++D+ELFSL+ IKGK DL  VD+ 
Sbjct: 361 TVDRKKKQAKKILAKRRAKDKARKATGPQMDVLEDGFVDNELFSLNAIKGKKDLMAVDN- 420

Query: 421 DYDVDNGELGEHENDVTNEEDHGSSA------SDIDSDEERRRYDEHMEELLDQAYESFV 480
           D D DNG   + EN     EDHG  A      SD DSDEER++Y E MEE+ +QAYE ++
Sbjct: 421 DED-DNGNAVDSEN-----EDHGEGASDDSKDSDRDSDEERQKYTEQMEEIFEQAYERYM 480

Query: 481 ARKEGSAKQRKRAKKAYSDDAELLEEDENGGDGFQSDYDSDENLVDEDRNPLMVSLDDGG 540
            +KEGSAKQRKRA++A+   AE LEE + G +  + DYDSD N   ++ NPL+V LDDG 
Sbjct: 481 VKKEGSAKQRKRARQAH---AEKLEEGD-GDEEMKIDYDSDMNEEKDEANPLVVPLDDGV 540

Query: 541 EPTQEEIANKWFGQDIFAEAAEDGDLKELDSEDDMQVDGPKEKTAVTKVAKSNISKNARE 600
             T+EEI+N+WF Q+IFAEA E+GDL + DSED++            K    N+SK  + 
Sbjct: 541 VQTKEEISNQWFSQNIFAEAVEEGDLGKDDSEDEI----------ANKKKSKNLSKPDKS 600

Query: 601 KSKIS-------TNAKDSIKADDGFEVVPAPATDSSNDSSSEESEDEEPDTKAEILACAK 660
           K K S        +  +S K +D FEVVPAPATDS +DSSSE    ++  TKAEILACAK
Sbjct: 601 KQKASKASVLSDQSLPNSSKKEDEFEVVPAPATDSDSDSSSE----DDVHTKAEILACAK 660

Query: 661 KMVRKKQREQILDDSYNKYMFDDTGLPKWFLDEEKRHRQPIKPITKEEVAAMRAQFKEID 720
           KM+RKKQREQ+LDD+YNK+MF D GLPKWF+D+EK+HRQP+KP+TK+EV AM+AQFKEI+
Sbjct: 661 KMLRKKQREQMLDDAYNKHMFVDEGLPKWFVDDEKQHRQPMKPVTKDEVNAMKAQFKEIN 720

Query: 721 ARPAKKVAEAKARKKRVAMKKLEKVRKKANIISDQADISDRSKSKMIDQLYKKAV-PQRP 780
           ARPAKKVAEAKARKKR A K+LEKVRKKAN ISD ADISDRSK KMID+LYKKA  P++P
Sbjct: 721 ARPAKKVAEAKARKKRAAQKRLEKVRKKANTISDTADISDRSKDKMIDKLYKKAAEPRKP 780

Query: 781 KKEFVVAKKGVQVRVGKGKTLVDRRMKKDARKHGMSKQGKGSKKGKNSKAPRGKGGSAKA 831
           +KE VV+KKGV V+VGKG+  VDRRMK D RK G  K G+  +KG      +GK  + K 
Sbjct: 781 RKELVVSKKGVGVKVGKGQKRVDRRMKSDDRKRGGGKPGRNGQKGTGKAGQKGKRPAGKP 819

BLAST of CmoCh20G008540 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 2.4e-31
Identity = 82/223 (36.77%), Postives = 126/223 (56.50%), Query Frame = 0

Query: 1   MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVA 60
           MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W QV 
Sbjct: 1   MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQVL 60

Query: 61  VER--VPVGS----------LVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMNEKG 120
             +  +P  S          L+V +DL P+AP+ G I ++ DIT    +     I +  G
Sbjct: 61  SRQLYLPAKSSAESKDGDLPLIVAIDLQPMAPIEGVIQVQGDIT--NARTAEVVIRHFDG 120

Query: 121 CAAFDLILHDGSPNVGGAWAQEAMSQNSLVIDSVKLATQLLAPKGAFVTKVFRSQDYSSV 180
           C A DL++ DG+P+V G    +   Q+ L++  + + T +L   G F+ K+FR +D +S+
Sbjct: 121 CKA-DLVVCDGAPDVTGLHDMDEFVQSQLILAGLTIVTHILKEGGKFIAKIFRGKD-TSL 180

Query: 181 LYC-LKQLFEKVEVDKPAASRSASAEIYVLGLRYTAPAKIDPR 211
           LYC LK  F  V   KP +SR++S E + +   Y+ P   +PR
Sbjct: 181 LYCQLKLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPR 218

BLAST of CmoCh20G008540 vs. TAIR 10
Match: AT5G13830.1 (FtsJ-like methyltransferase family protein )

HSP 1 Score: 68.9 bits (167), Expect = 2.1e-11
Identity = 58/212 (27.36%), Postives = 95/212 (44.81%), Query Frame = 0

Query: 11  DKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQVAVERV-PV--G 70
           D +YR A+  GY +R+++KL Q+  +Y  ++   +VLDL  APG W+QVA + + P+  G
Sbjct: 8   DFFYREAQRLGYVARSAFKLLQIQKQYKLIKPGSSVLDLGCAPGAWLQVACQSLGPLRSG 67

Query: 71  SLVVGVDLVPIAPVRGAIAIEQDITKPECKARLKKIMN-EKGCAAFDLILHDGSPNVGGA 130
            +VVG+D+  +       +  Q I         +KI         F +IL D   +V G 
Sbjct: 68  GIVVGMDIKKVKVPPQCDSRVQTIAADVLNFPRQKIRELSPQQLGFSVILSDMCHSVSGI 127

Query: 131 WAQEAMSQNSLVIDSVKLAT---------------------QLLAPKGAFVTKVFRSQDY 190
             ++A     L + ++ LA                       +L   G  V K+  S+D 
Sbjct: 128 TTRDAALSAELGMRALDLAVGQAAISQSPNDDDGGPNESRPGVLRHGGHLVIKLLESEDA 187

Query: 191 SSVLYCLKQLFEKVEVDKPAASRSASAEIYVL 198
                  K +F K    +P A+R +S EIY++
Sbjct: 188 QDFARICKPIFNKASWLRPKATRPSSREIYLI 219

BLAST of CmoCh20G008540 vs. TAIR 10
Match: AT5G01230.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 7.6e-09
Identity = 28/58 (48.28%), Postives = 39/58 (67.24%), Query Frame = 0

Query: 1  MGKVKGKHRLDKYYRLAKEHGYRSRASWKLAQLDSKYNFLRSSHAVLDLCAAPGGWMQ 59
          MGK   + + D YYR AKE G+R+R+++KL Q+D ++N       V+DLCAAPG W Q
Sbjct: 1  MGKA-SRDKRDIYYRKAKEEGWRARSAFKLLQIDEEFNIFEGVKRVVDLCAAPGSWSQ 57