CmoCh20G008450 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh20G008450
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionBifunctional dihydrofolate reductase-thymidylate synthase
LocationCmo_Chr20: 4256776 .. 4257991 (+)
RNA-Seq ExpressionCmoCh20G008450
SyntenyCmoCh20G008450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTCAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAACATGGGTATTGGTAAGGATGGGAAACTACCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCGCGACAACATCAGATCATGGGAAAAGGAATGCGATTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGCTCTGGAAGTTTCGACATTGCCACCGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCACCTTATTGTTTGTCAATAGAGAAAGTGTTTGTCATAGGTGGTGGTGAGATATTAAGGTGTGCACTTGTTTTCGAGACCTTCGTTAGTGTACTATTTTTTGTTTCGGCGCACTGATTGTGTTTTTAATGCAGGGAAGCTCTGAACGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGTGACACCTTCATACCAGCTATCGACAAGTCTATGTTTCAGCCATGGTACTCGTCCTTTCCCGTGGTGGAAAATAACTTACGATATTCATTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCCATTGATCAGAACAATGGTCTGGTCTCTCACAACAAACCAGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTCGAGAGGCACGAGGAATACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGGTAACGTTATCTTTCTTATTATTTGACATATTTATCTTTTATACAAGTTGCTCTATTTCGAATGCTACACGTTCATCTCTTGTTCGCGGTATCTGTACAGATGCGGTACAACTTGCGCAGAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGGTTTCCTTTGTAGCTGTGGCATGAAAGAAAAACTCCTTTTTTTACTCGTCTTGTTTCCGTTTTTAAATTTCTTAAATCGGGTGCATCGTATATGGTAATGGTTTAGTCATTGACATATATTCGTACCACGTGATCCTTCAGA

mRNA sequence

ATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTCAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAACATGGGTATTGGTAAGGATGGGAAACTACCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCGCGACAACATCAGATCATGGGAAAAGGAATGCGATTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGCTCTGGAAGTTTCGACATTGCCACCGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCACCTTATTGTTTGTCAATAGAGAAAGTGTTTGTCATAGGTGGTGGTGAGATATTAAGGGAAGCTCTGAACGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGTGACACCTTCATACCAGCTATCGACAAGTCTATGTTTCAGCCATGGTACTCGTCCTTTCCCGTGGTGGAAAATAACTTACGATATTCATTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCCATTGATCAGAACAATGGTCTGGTCTCTCACAACAAACCAGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTCGAGAGGCACGAGGAATACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAGAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGA

Coding sequence (CDS)

ATGGCTGCTGATCCTCTGACAAGCTCGAATGGAAATGCCAATGTCAATGCACCACCTAATCCGCGAAGGACTTATCAAGTCGTTGTAGCTGCTACTGAAAACATGGGTATTGGTAAGGATGGGAAACTACCATGGAGGTTGCCTTCTGATCTCAAATTTTTTAAGAACATAACCGCGACAACATCAGATCATGGGAAAAGGAATGCGATTCTAATGGGTCGTAAAACATGGGAGAGTATTCCGGCTGAGTATCGACCTCTACCTGGTCGTCTCAATGTTGTTCTGACTCGCTCTGGAAGTTTCGACATTGCCACCGTCGAAAATGTTATAATATGTGGTAGTATGACGTCTGCTTTGGAATTATTAGCAGCTTCACCTTATTGTTTGTCAATAGAGAAAGTGTTTGTCATAGGTGGTGGTGAGATATTAAGGGAAGCTCTGAACGCCCCCGAGTGTGATGCGGTCCACGTTACTGAAATCGAGACAAACGTTGAATGTGACACCTTCATACCAGCTATCGACAAGTCTATGTTTCAGCCATGGTACTCGTCCTTTCCCGTGGTGGAAAATAACTTACGATATTCATTCACTAGTTATGTTCGTGTGAGGAGCTCAACCGAACCACCCATTGATCAGAACAATGGTCTGGTCTCTCACAACAAACCAGATTCTTTTAACTTCGAAGCTAAGAACTTCTCTTTCCTACCCAAGGAGATTTTCGAGAGGCACGAGGAATACTTGTACCTAAAACTTGTTCAAGAAATCATCTCAGACGGTACTTCCAAGGATGATAGAACGGGAACGGGTACTTTATCAAAATTTGGTTGCCAGATGCGGTACAACTTGCGCAGAACTTTTCCGCTGCTTACAACTAAGGCATGTGCTCGTTTCGTATTTTGA

Protein sequence

MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKACARFVF
Homology
[Close]
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match: P45350 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 441.4 bits (1134), Expect = 8.0e-123
Identity = 210/292 (71.92%), Postives = 251/292 (85.96%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MA++ L +    + +   P+P+RTYQVVVAAT+NMGIGKDGKLPWRLPSD+KFFK++T T
Sbjct: 1   MASELLANPTNGSGITR-PDPQRTYQVVVAATQNMGIGKDGKLPWRLPSDMKFFKDVTMT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD  KRNA++MGRKTWESIP ++RPLPGRLNVVLTRSGSFDIATVENV+ICGSM SALE
Sbjct: 61  TSDPLKRNAVIMGRKTWESIPIQHRPLPGRLNVVLTRSGSFDIATVENVVICGSMISALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLA SPYC+S+EKVFVIGGG+I REALNAP CDAVH+TEIE ++ECDTFIP +D+S+FQP
Sbjct: 121 LLAGSPYCVSVEKVFVIGGGQIYREALNAPGCDAVHITEIEEHIECDTFIPLLDESVFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFP+VEN +RY FT+YVRVR+S      Q NG  S +K DS NFE +NFSFLPK +F
Sbjct: 181 WYSSFPLVENKIRYCFTTYVRVRNSVAELTSQTNGCSSDSKSDSGNFEIQNFSFLPKTVF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           E+HEEYLYL LV+ IIS+G +K+DRT TGT+S FGCQMR+NLR++FPLLTTK
Sbjct: 241 EKHEEYLYLGLVENIISNGVTKNDRTRTGTVSIFGCQMRFNLRKSFPLLTTK 291

[Close]
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 9.8e-121
Identity = 213/294 (72.45%), Postives = 248/294 (84.35%), Query Frame = 0 

Query: 1   MAADPLTSSNGNAN--VNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT 60
           M +D    SNG++N  VN  PN +RTYQVVVAAT++ GIGKDGKLPWRLP+DLKFFK IT
Sbjct: 1   MPSDSSVISNGHSNGSVNPLPNLQRTYQVVVAATQDWGIGKDGKLPWRLPTDLKFFKEIT 60

Query: 61  ATTSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSA 120
             TS+ GK+NAI+MGRKTWESIP EYRPL GRLNVVLTRSGSFDIAT ENV+ICGSM+SA
Sbjct: 61  MKTSEPGKKNAIVMGRKTWESIPLEYRPLSGRLNVVLTRSGSFDIATAENVVICGSMSSA 120

Query: 121 LELLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMF 180
           LELLAASPY LSIEKVFVIGGG+I REALN P C+A+H+TEI++++ECDTF+P +D ++F
Sbjct: 121 LELLAASPYSLSIEKVFVIGGGQIFREALNVPGCEAIHLTEIQSSIECDTFMPPVDFTIF 180

Query: 181 QPWYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKE 240
           +PWYSSFP VENN+RYSFT+YVRVRSS      QN   +  N  +S  FE K+FSFLPK 
Sbjct: 181 RPWYSSFPKVENNIRYSFTTYVRVRSSAAESAGQNIDPLLDNNSESMKFEVKDFSFLPKM 240

Query: 241 IFERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           I ERHEEYLYLKLVQ+II++GT+K DRTGTGTLSKFGCQMR+NLR  FPLLTTK
Sbjct: 241 ISERHEEYLYLKLVQDIIAEGTTKGDRTGTGTLSKFGCQMRFNLRGNFPLLTTK 294

[Close]
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 406.4 bits (1043), Expect = 2.9e-112
Identity = 192/286 (67.13%), Postives = 234/286 (81.82%), Query Frame = 0 

Query: 7   TSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGK 66
           T+ N +        P+RTYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASP 126
           +NA++MGRKTWESIP +YRPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREALN P CDA+H+TEI+T+V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEY 246
           V EN LR+ FT++VRV+SS +   D++NG        S  F+ K F FLPK +F++HEE+
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNG------SQSLQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           LYL +V++IIS+G  K+DRTGTGTLSKFGCQM++NLRR+FPLLTTK
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTK 283

[Close]
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 399.4 bits (1025), Expect = 3.5e-110
Identity = 186/280 (66.43%), Postives = 229/280 (81.79%), Query Frame = 0 

Query: 13  ANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGKRNAILM 72
           AN ++   P+R YQVVVA T +MGIGKDG LPW+LP DLKFFK +T TTSD  K+NA++M
Sbjct: 6   ANGDSQGRPQRNYQVVVAGTRDMGIGKDGVLPWKLPGDLKFFKELTLTTSDPVKKNAVIM 65

Query: 73  GRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSIE 132
           GRKTWESIP + RPLPGRLNV+LTRSGSFD ATVENV+ICGSM SALELLA++PYCLSIE
Sbjct: 66  GRKTWESIPVKSRPLPGRLNVILTRSGSFDFATVENVVICGSMESALELLASTPYCLSIE 125

Query: 133 KVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENNL 192
           KVFVIGGG++LRE L  P C+A+H+T+I++++ECDTFIP +D S+FQPWYSSFPV+E+N+
Sbjct: 126 KVFVIGGGQVLREYLKGPACEAIHLTDIQSSIECDTFIPPVDFSVFQPWYSSFPVIESNI 185

Query: 193 RYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYLYLKLV 252
           R+SF S+VRVR S     + N    +     +  FE +NFSFLPK +++RHEEY YL LV
Sbjct: 186 RHSFVSFVRVRKSVAETHESNGKESTEVDTKNDKFETENFSFLPKMVYDRHEEYQYLNLV 245

Query: 253 QEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ++II  G  K+DRTGTGTLSKFGCQMR+NLR+ FPLLTTK
Sbjct: 246 EDIIRSGAQKNDRTGTGTLSKFGCQMRFNLRKNFPLLTTK 285

[Close]
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match: Q2QRX6 (Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0446900 PE=3 SV=2)

HSP 1 Score: 386.3 bits (991), Expect = 3.1e-106
Identity = 178/258 (68.99%), Postives = 215/258 (83.33%), Query Frame = 0 

Query: 35  MGIGKDGKLPWRLPSDLKFFKNITATTSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVV 94
           MGIGKDG LPW+LP DLKFFK+IT TTSD  K+NA++MGRKTWESIP ++RPLPGRLNV+
Sbjct: 1   MGIGKDGTLPWKLPGDLKFFKDITVTTSDPSKKNAVVMGRKTWESIPLKFRPLPGRLNVI 60

Query: 95  LTRSGSFDIATVENVIICGSMTSALELLAASPYCLSIEKVFVIGGGEILREALNAPECDA 154
           LTRSGSFD AT ENV+ICGS+ SAL+LLA +PYCL++EK F+IGGGEILR++LNAP C+A
Sbjct: 61  LTRSGSFDFATAENVVICGSLDSALQLLATTPYCLTVEKTFIIGGGEILRQSLNAPACEA 120

Query: 155 VHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNN 214
           +H+T+IE+++ECDTFIP ID SMF PWYSSFPVVEN +++SF S+VRV  S     D + 
Sbjct: 121 IHLTDIESSIECDTFIPPIDLSMFHPWYSSFPVVENGIKHSFISFVRVTKSIAEANDSSG 180

Query: 215 GLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKF 274
             ++ N      FE +NFSFLPK IFERHEEY YL LVQ+II +G  K+DRTGTGT+SKF
Sbjct: 181 KELTGNDSKKVKFEIENFSFLPKMIFERHEEYQYLNLVQDIIRNGAKKNDRTGTGTVSKF 240

Query: 275 GCQMRYNLRRTFPLLTTK 293
           GCQMR+NLRR FPLLTTK
Sbjct: 241 GCQMRFNLRRNFPLLTTK 258

[Close]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.1e-166
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 292

[Close]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match: A0A6J1J7P4 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111484218 PE=3 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 9.1e-162
Identity = 285/292 (97.60%), Postives = 288/292 (98.63%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFP+VENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN   KNFSFLPKEIF
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN---KNFSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK 289

[Close]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match: A0A6J1CYC5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia OX=3673 GN=LOC111015400 PE=3 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 4.5e-153
Identity = 267/292 (91.44%), Postives = 283/292 (96.92%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MA DPLTSSNGNANV APP+PRRT+QVVVAATENMGIGKDGKLPWRLPSDLK+FKNIT T
Sbjct: 10  MAGDPLTSSNGNANVIAPPDPRRTFQVVVAATENMGIGKDGKLPWRLPSDLKYFKNITTT 69

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 70  TSDHGKRNAVLMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 129

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVF+IGGGEILREALNAPECDA+HVT+I+T VECDTFIPAIDKSMFQP
Sbjct: 130 LLAASPYCLSIEKVFIIGGGEILREALNAPECDAIHVTDIQTCVECDTFIPAIDKSMFQP 189

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSS EPPIDQNNGLVS+++PDS NFEAK FSFLPKEIF
Sbjct: 190 WYSSFPVVENNLRYSFTSYVRVRSSIEPPIDQNNGLVSYSRPDSSNFEAKKFSFLPKEIF 249

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYL LV+EIISDGTSKDDRTGTGTLS+FG QMRYNLR+TFPLLTTK
Sbjct: 250 ERHEEYLYLNLVKEIISDGTSKDDRTGTGTLSRFGVQMRYNLRKTFPLLTTK 301

[Close]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match: A0A0A0LPA5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=3659 GN=Csa_2G248730 PE=3 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 6.5e-152
Identity = 267/292 (91.44%), Postives = 280/292 (95.89%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNG ANVN+PP+PRRTYQVVVAATEN+GIGK GKLPWRLPSD+KFFKNIT T
Sbjct: 1   MAADPLTSSNGIANVNSPPDPRRTYQVVVAATENLGIGKGGKLPWRLPSDIKFFKNITIT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD GKRNA+LMGRKTWES+PAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDPGKRNAVLMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREA+NAPECDAVH+TEIETNVECDTFIPA+DKS+FQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNVECDTFIPAVDKSIFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVE NLRYSF SYVRVRSS EPPIDQNNGLVSHNKPD  NFEAK FSFLPKEIF
Sbjct: 181 WYSSFPVVEKNLRYSFNSYVRVRSSAEPPIDQNNGLVSHNKPDFSNFEAK-FSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDG  KDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK
Sbjct: 241 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK 291

[Close]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match: A0A5A7TVM8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00740 PE=3 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.5e-151
Identity = 267/293 (91.13%), Postives = 278/293 (94.88%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLT SNG ANVN+ P+PRRTYQVVVAATEN+GIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 49  MAADPLTISNGIANVNSLPDPRRTYQVVVAATENLGIGKDGKLPWRLPSDLKFFKNITTT 108

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSD GKRNAILMGRKTWES+PAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+ SALE
Sbjct: 109 TSDPGKRNAILMGRKTWESLPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSIMSALE 168

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREA+NAPECDAVH+TEIETN+ECDTFIPAIDK+MFQP
Sbjct: 169 LLAASPYCLSIEKVFVIGGGEILREAMNAPECDAVHITEIETNIECDTFIPAIDKAMFQP 228

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSF SYVRVRSS E PIDQNNGLVSHNKPD  N EAK FSFLPKEIF
Sbjct: 229 WYSSFPVVENNLRYSFNSYVRVRSSAELPIDQNNGLVSHNKPDFSNIEAKKFSFLPKEIF 288

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTKA 294
           ERHEEYLYLKLVQEIISDG  KDDRTGTGTLSKFGCQMRYNLR+TFPLLTTKA
Sbjct: 289 ERHEEYLYLKLVQEIISDGIPKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTKA 341

[Close]
BLAST of CmoCh20G008450 vs. NCBI nr
Match: XP_022944443.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata] >XP_022944444.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata])

HSP 1 Score: 594.7 bits (1532), Expect = 4.3e-166
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 292

[Close]
BLAST of CmoCh20G008450 vs. NCBI nr
Match: KAG6571099.1 (hypothetical protein SDJN03_30014, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 592.8 bits (1527), Expect = 1.6e-165
Identity = 291/292 (99.66%), Postives = 292/292 (100.00%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT
Sbjct: 568 MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 627

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 628 TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 687

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAI+KSMFQP
Sbjct: 688 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAINKSMFQP 747

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF
Sbjct: 748 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 807

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK
Sbjct: 808 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 859

[Close]
BLAST of CmoCh20G008450 vs. NCBI nr
Match: XP_023513067.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo] >XP_023513068.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 9.0e-164
Identity = 289/293 (98.63%), Postives = 291/293 (99.32%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTE-PPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240
           WYSSFPVVENNLRYSFTSYVRVRSSTE PPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEI 240

Query: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK
Sbjct: 241 FERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK 293

[Close]
BLAST of CmoCh20G008450 vs. NCBI nr
Match: XP_022986497.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima])

HSP 1 Score: 579.3 bits (1492), Expect = 1.9e-161
Identity = 285/292 (97.60%), Postives = 288/292 (98.63%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNA+LMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE
Sbjct: 61  TSDHGKRNAVLMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFP+VENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN   KNFSFLPKEIF
Sbjct: 181 WYSSFPMVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFN---KNFSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLR+TFPLLTTK
Sbjct: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRKTFPLLTTK 289

[Close]
BLAST of CmoCh20G008450 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 270/292 (92.47%), Postives = 283/292 (96.92%), Query Frame = 0 

Query: 1   MAADPLTSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITAT 60
           MA DPLTSSNG ANVNAPP+PRRTYQVVVAA+ENMGIGKDGKLPWRLPSDLKFFKNIT T
Sbjct: 1   MAGDPLTSSNGIANVNAPPDPRRTYQVVVAASENMGIGKDGKLPWRLPSDLKFFKNITTT 60

Query: 61  TSDHGKRNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALE 120
           TSDHGKRNAILMGRKTWESIPAE+RPLPGRLNVVLTRSGSFDIATVENVIICGS+TSALE
Sbjct: 61  TSDHGKRNAILMGRKTWESIPAEHRPLPGRLNVVLTRSGSFDIATVENVIICGSITSALE 120

Query: 121 LLAASPYCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQP 180
           LLAASPYCLSIEKVFV+GGGEILREALNAPECDA+H+TE+ETN+ECDTFIPAIDKSMFQP
Sbjct: 121 LLAASPYCLSIEKVFVVGGGEILREALNAPECDAIHITEVETNIECDTFIPAIDKSMFQP 180

Query: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIF 240
           WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPD  NFEAK FSFLPKEIF
Sbjct: 181 WYSSFPVVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDFSNFEAKKFSFLPKEIF 240

Query: 241 ERHEEYLYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           E+HEEYLYL LVQEIIS+G  KDDRTGTGTLS+FGCQMRYNLR+TFPLLTTK
Sbjct: 241 EKHEEYLYLNLVQEIISNGIPKDDRTGTGTLSRFGCQMRYNLRKTFPLLTTK 292

[Close]
BLAST of CmoCh20G008450 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 406.4 bits (1043), Expect = 2.0e-113
Identity = 192/286 (67.13%), Postives = 234/286 (81.82%), Query Frame = 0 

Query: 7   TSSNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGK 66
           T+ N +        P+RTYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK+IT TTSD  K
Sbjct: 4   TTLNDSVTTTLASEPQRTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDITLTTSDSSK 63

Query: 67  RNAILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASP 126
           +NA++MGRKTWESIP +YRPL GRLNVVLTRSG FDIA  ENV+ C S+ SAL+LLAA P
Sbjct: 64  KNAVVMGRKTWESIPIKYRPLSGRLNVVLTRSGGFDIANTENVVTCSSVDSALDLLAAPP 123

Query: 127 YCLSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFP 186
           YCLSIE+VFVIGGG+ILREALN P CDA+H+TEI+T+V+CDTFIPAID S++QPW SSFP
Sbjct: 124 YCLSIERVFVIGGGDILREALNRPSCDAIHLTEIDTSVDCDTFIPAIDTSVYQPWSSSFP 183

Query: 187 VVENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEY 246
           V EN LR+ FT++VRV+SS +   D++NG        S  F+ K F FLPK +F++HEE+
Sbjct: 184 VTENGLRFCFTTFVRVKSSADESSDESNG------SQSLQFDGKKFLFLPKMVFDQHEEF 243

Query: 247 LYLKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           LYL +V++IIS+G  K+DRTGTGTLSKFGCQM++NLRR+FPLLTTK
Sbjct: 244 LYLNMVEDIISNGNVKNDRTGTGTLSKFGCQMKFNLRRSFPLLTTK 283

[Close]
BLAST of CmoCh20G008450 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 379.8 bits (974), Expect = 2.0e-105
Identity = 186/283 (65.72%), Postives = 226/283 (79.86%), Query Frame = 0 

Query: 12  NANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGKRNAIL 71
           N NV     P+ TYQVVVAAT+ MGIGKDGKLPW LP+DLKFFK++T +TSD  K+NA++
Sbjct: 53  NGNVIMTSKPQSTYQVVVAATKEMGIGKDGKLPWNLPTDLKFFKDLTLSTSDSAKKNAVV 112

Query: 72  MGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYCLSI 131
           MGRKTWESIP +YRPL GRLNVVL+RS  FDIA  ENV+ C S+ SAL+LLAA P+ LSI
Sbjct: 113 MGRKTWESIPKKYRPLSGRLNVVLSRSSGFDIANTENVVTCSSIDSALDLLAAPPFSLSI 172

Query: 132 EKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVVENN 191
           EKVFVIGGG+ILREALN P C+A+H+TEI+T+++CDTFIP +D S +QPW SSFP+ EN 
Sbjct: 173 EKVFVIGGGDILREALNKPSCEAIHITEIDTSIDCDTFIPTVDTSAYQPWCSSFPICENG 232

Query: 192 LRYSFTSYVRVRSSTE-PPIDQNNGLVSHNKPDSFNFEAKNF-SFLPKEIFERHEEYLYL 251
           LR+SFT++VRV+SS+     D+++G            + K F S LPK IF+RHEEYLYL
Sbjct: 233 LRFSFTTHVRVKSSSAGEASDESDG------SKVLQVDWKKFSSVLPKMIFDRHEEYLYL 292

Query: 252 KLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
            LV+EIIS+G  KDDRTGTGTLSKFGCQM++NLRR FPLLTTK
Sbjct: 293 NLVKEIISNGNLKDDRTGTGTLSKFGCQMKFNLRRNFPLLTTK 329

[Close]
BLAST of CmoCh20G008450 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 325.5 bits (833), Expect = 4.5e-89
Identity = 164/284 (57.75%), Postives = 205/284 (72.18%), Query Frame = 0 

Query: 9   SNGNANVNAPPNPRRTYQVVVAATENMGIGKDGKLPWRLPSDLKFFKNITATTSDHGKRN 68
           +NG + V+     RR+YQVV+AAT +MG+G D KLPW LPS+ +FF+++T  TSD  KRN
Sbjct: 10  NNGQSAVSV--TRRRSYQVVIAATRDMGLGMDMKLPWDLPSEYQFFQDVTTRTSDPTKRN 69

Query: 69  AILMGRKTWESIPAEYRPLPGRLNVVLTRSGSFDIATVENVIICGSMTSALELLAASPYC 128
           A +MGRK+WES P E RPLPGRLN+VLT+S   +IA  ENV++  SM SALELLA  PY 
Sbjct: 70  ATIMGRKSWESTPLEIRPLPGRLNIVLTKSSCHNIAIDENVLVSSSMESALELLATEPYS 129

Query: 129 LSIEKVFVIGGGEILREALNAPECDAVHVTEIETNVECDTFIPAIDKSMFQPWYSSFPVV 188
           LSIEKVFVIGGGE+LR  +NA  CDA+H+TEI+ +V CD F P +D S+++PWYSSFPVV
Sbjct: 130 LSIEKVFVIGGGELLRNYMNASICDAIHLTEIDISVPCDAFAPRVDTSLYRPWYSSFPVV 189

Query: 189 ENNLRYSFTSYVRVRSSTEPPIDQNNGLVSHNKPDSFNFEAKNFSFLPKEIFERHEEYLY 248
           EN +RYSF +YVR + +          + S  K      + K +SFLPK +FERHEE+ Y
Sbjct: 190 ENGIRYSFNTYVRRKDAI---------VGSGEKKSVAESDLKEYSFLPKMVFERHEEFGY 249

Query: 249 LKLVQEIISDGTSKDDRTGTGTLSKFGCQMRYNLRRTFPLLTTK 293
           L LVQ IIS G   D+     TLSKFGCQMR+NLR+TFPLLTTK
Sbjct: 250 LNLVQNIISSGDMNDN----STLSKFGCQMRFNLRKTFPLLTTK 278

The following BLAST results are available for this feature:
BLAST of CmoCh20G008450 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P453508.0e-12371.92Bifunctional dihydrofolate reductase-thymidylate synthase OS=Daucus carota OX=40... [more]
P518209.8e-12172.45Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847... [more]
Q057622.9e-11267.13Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali... [more]
O813953.5e-11066.43Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN... [more]
Q2QRX63.1e-10668.99Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sati... [more]
BLAST of CmoCh20G008450 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FWP82.1e-166100.00Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ... [more]
A0A6J1J7P49.1e-16297.60Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX... [more]
A0A6J1CYC54.5e-15391.44Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia... [more]
A0A0A0LPA56.5e-15291.44Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis sativus OX=... [more]
A0A5A7TVM82.5e-15191.13Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m... [more]
BLAST of CmoCh20G008450 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022944443.14.3e-166100.00bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moscha... [more]
KAG6571099.11.6e-16599.66hypothetical protein SDJN03_30014, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023513067.19.0e-16498.63bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo s... [more]
XP_022986497.11.9e-16197.60bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima... [more]
XP_038901596.11.2e-15592.47bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp... [more]
BLAST of CmoCh20G008450 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G16370.12.0e-11367.13thymidylate synthase 1 [more]
AT4G34570.12.0e-10565.72thymidylate synthase 2 [more]
AT2G21550.14.5e-8957.75Bifunctional dihydrofolate reductase/thymidylate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00070DHFRcoord: 36..46
score: 58.68
coord: 50..58
score: 46.75
coord: 133..147
score: 43.38
coord: 70..81
score: 65.15
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR11548:SF12BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASEcoord: 1..293
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3D3.30.572.10Thymidylate synthase/dCMP hydroxymethylase domaincoord: 241..296
e-value: 1.6E-16
score: 62.0
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilySUPERFAMILY55831Thymidylate synthase/dCMP hydroxymethylasecoord: 224..295
IPR024072Dihydrofolate reductase-like domain superfamilyGENE3D3.40.430.10Dihydrofolate Reductase, subunit Acoord: 8..203
e-value: 1.3E-63
score: 216.5
IPR024072Dihydrofolate reductase-like domain superfamilySUPERFAMILY53597Dihydrofolate reductase-likecoord: 20..201
IPR001796Dihydrofolate reductase domainPFAMPF00186DHFR_1coord: 26..201
e-value: 8.8E-39
score: 132.8
IPR001796Dihydrofolate reductase domainPROSITEPS51330DHFR_2coord: 24..201
score: 52.322918
IPR001796Dihydrofolate reductase domainCDDcd00209DHFRcoord: 28..200
e-value: 1.4229E-63
score: 195.82
IPR023451Thymidylate synthase/dCMP hydroxymethylase domainPFAMPF00303Thymidylat_syntcoord: 248..294
e-value: 3.1E-13
score: 49.5
IPR045097Thymidylate synthase/dCMP hydroxymethylasePANTHERPTHR11548THYMIDYLATE SYNTHASE 1coord: 1..293
IPR017925Dihydrofolate reductase conserved sitePROSITEPS00075DHFR_1coord: 36..58

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh20G008450.1CmoCh20G008450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006231 dTMP biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0046654 tetrahydrofolate biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004146 dihydrofolate reductase activity
molecular_function GO:0004799 thymidylate synthase activity
molecular_function GO:0016741 transferase activity, transferring one-carbon groups