CmoCh20G002830 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh20G002830
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	X8 domain-containing protein
Location	Cmo_Chr20: 1374944 .. 1376581 (-)
RNA-Seq Expression	CmoCh20G002830
Synteny	CmoCh20G002830

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCTCACCGCATCAAGTTCCCACTCACAAATCGTGTTCACTTCTTCTTCGTCGTCCTGTGTCTCAATCTCAGAGACACCCACCTCTACAACTCCGACCACCACCACCTTCCGATCATCGGCGGAGGAAACAGAGAGCGTCTCTGCGAATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTCAGAACTCTAATAAGCTCATCTTCTTTTCATGTTTTCTTCAATCAATGAATCCATTAAAGTAGTTCTTGTTCTTCTGTCCTGTTCTTGGATCTTTTCTTTTATGCTTTTCATGGTGTCGACTTCAATTTTGAATGCCTAAAAATGGGTTTGTTGTTTTGTTTGTACCTTTTTCCCCTAAATGGAAAACAGGTTCATCGAGAGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCGACAATCAACATCCCCACTACTTCCAACCCGGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTACAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAGAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACTAATCCCAGTAAGCTATCTTTGTCGCTTTCGCTAATTAGGGTTTATGAATTGATCCATGGTTTTGGTTAGGTGAGTAATAATTGTGTTTTTTAATGTTGTCACAGGCAGTGGCTCGTGCGAGTACCCATCAACAAGGTGAGCCCTCTGCAACCCGTTTGATCAATAAATTTTTGAAATGAAATTAAAATGTTAGTTGAAATTGAATTTATTTGCATTTGCATTTGCATTTGCAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAGAAAGTTCTGCAATTTCTCAACGATTTTTGTAAAGTAATGTTGGGCCGGAATATCTTTATAGAGAAGAGTCTGATGCATCTCTGGAGGCCCAAGAACGGTGTGCTTAATGGGCTTTGAAAAGCCCATTTGAAAATTCGTGGCTATGTGGGCCCTACAATCGTCAAACAGAAGCAAACCCGAAGCGGAAGCCAGGTATATTCCCTTCATCGGCCCTTTTTATCTTTTTTCGCCACCACGCAAGGGGAAGGGCAAATGCGTCAATTCACCGTCTCTTGGGCCTTTACGTCACTCTTACACTACTATATTCTTCCTCCCATTATATATATATATAAATAAAATAAATAATTGTAGAAGTCAAAGGAACCTAGACAGAATTTGTATATTAATCATTTAATCTTAGCTTACCTAATTTCATTAAATAAAATCCCCAACTTA

mRNA sequence

CGCTCACCGCATCAAGTTCCCACTCACAAATCGTGTTCACTTCTTCTTCGTCGTCCTGTGTCTCAATCTCAGAGACACCCACCTCTACAACTCCGACCACCACCACCTTCCGATCATCGGCGGAGGAAACAGAGAGCGTCTCTGCGAATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTTCATCGAGAGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCGACAATCAACATCCCCACTACTTCCAACCCGGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTACAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAGAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACTAATCCCAGCAGTGGCTCGTGCGAGTACCCATCAACAAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAGAAAGTTCTGCAATTTCTCAACGATTTTTGTAAAGTAATGTTGGGCCGGAATATCTTTATAGAGAAGAGTCTGATGCATCTCTGGAGGCCCAAGAACGGTGTGCTTAATGGGCTTTGAAAAGCCCATTTGAAAATTCGTGGCTATGTGGGCCCTACAATCGTCAAACAGAAGCAAACCCGAAGCGGAAGCCAGGTATATTCCCTTCATCGGCCCTTTTTATCTTTTTTCGCCACCACGCAAGGGGAAGGGCAAATGCGTCAATTCACCGTCTCTTGGGCCTTTACGTCACTCTTACACTACTATATTCTTCCTCCCATTATATATATATATAAATAAAATAAATAATTGTAGAAGTCAAAGGAACCTAGACAGAATTTGTATATTAATCATTTAATCTTAGCTTACCTAATTTCATTAAATAAAATCCCCAACTTA

Coding sequence (CDS)

ATGGATGGGGGTTTCACTTGTACCACTCTAATCCTGTTCCTGTTCCTCTTCATTCACTGTCTTTTCCCAGGTTCATCGAGAGCAGATCCCACGCAAAAACAGGGCAAATACAGAGCAGCCTCTTCGACCCATCAAAAGGACATCACCACCCCAATAACCACCGTCCCGACAATCAACATCCCCACTACTTCCAACCCGGATTCCGTCTCCCCGACCATCACAACCCCAAGCTTTACCCCTGCAACCACCGCCACCGGTGGCTCCAGCTGGTGCATTGCCAGTCTCGGCGCATCGCAGGCAGTCTTACAACTAGCTCTGGACTACGCTTGCGGCATGGGCGGCGCCGATTGCTCCGCGATTCAAACCGGTGGAAGCTGCTACAACCCAAATTCAGTTCGAGATCATGCTTCTTACGCCTTCAATAGCTATTATCAGAAGAACCCACTTCCGAATAGCTGTAATTTTGGAGGCACTGCCGTGATCACCAGCACTAATCCCAGCAGTGGCTCGTGCGAGTACCCATCAACAAGCACGAGTTCATCGATTCTCAACACTACAAATTCAAGCGGCTCCACCGTGTTCGGCGCTGCTCCTTCCGGCCCCTCCACCTCCTCGTCGGCACCCAAATACTCCTCCTCCGCCCTCTTCCTTCTTCTTATTGCTTTCTAG

Protein sequence

MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINIPTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF

Homology

[Close]

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 3.0e-18
Identity = 55/126 (43.65%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 51  PITTVPTINIPTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYAC 110
           P+ T PT ++PT+S+P    PT             G   WC+    A+ A LQ  +DY C
Sbjct: 11  PVPT-PTPSVPTSSSPVPKPPT------------QGNKKWCVPKAEATDAQLQSNIDYVC 70

Query: 111 GMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-KNPLPNSCNFGGTAVITSTNPSSG 170
              G DC  IQ  G+C+NPN+VR HASYA NS+YQ K      C+F GT  ITS++PS+G
Sbjct: 71  SQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSKGRNDFDCDFSGTGAITSSDPSNG 123

Query: 171 SCEYPS 176
           SC + S
Sbjct: 131 SCSFLS 123

[Close]

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 93.2 bits (230), Expect = 4.0e-18
Identity = 55/116 (47.41%), Postives = 71/116 (61.21%), Query Frame = 0


Query: 85  TGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYY 144
           T   ++CIA  G     LQ ALD+ACG G ++CS IQ G SCY PN+V+ HAS+AFNSYY
Sbjct: 376 TTNQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYY 435

Query: 145 QK-NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAPS 200
           QK      SC+F G A+IT+T+PS GSC +P +    +   T  +S     G A S
Sbjct: 436 QKEGRASGSCDFKGVAMITTTDPSHGSCIFPGSKKVGNRTQTVVNSTEVAAGEATS 491

[Close]

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 4.0e-18
Identity = 56/128 (43.75%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 85  TGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYY 144
           T   ++CIA     + +LQ ALD+ACG G  DCSA+  G SCY P+ V  H++YAFN+YY
Sbjct: 355 TTNQTFCIAKEKVDRKMLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYY 414

Query: 145 QK-NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAPSGPST 204
           QK      SC+F G A +T+T+PS G+C +P ++ S+  L      G+     APS  ST
Sbjct: 415 QKMGKASGSCDFKGVATVTTTDPSRGTCVFPGSAKSNQTL------GNNTSALAPSANST 474

Query: 205 SSSA-PKY 211
           +S   PKY
Sbjct: 475 TSGCIPKY 476

[Close]

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot
Match: Q9SD84 (PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.2e-18
Identity = 55/120 (45.83%), Postives = 74/120 (61.67%), Query Frame = 0


Query: 88  SSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-K 147
           +SWC+   G S +VLQ  LDYACG  GADC+     GSC+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDSVLQKTLDYACG-NGADCNPTHPKGSCFNPDNVRAHCNYAVNSFFQKK 78

Query: 148 NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAPSGPSTSSS 207
                SCNF GTA +T+T+PS   C +PS+++ SS       SGST      + P  S+S
Sbjct: 79  GQASESCNFTGTATLTTTDPSYTGCAFPSSASGSS------GSGSTTVTPGKNSPKGSNS 131

[Close]

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot
Match: Q9FNQ2 (PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.4e-17
Identity = 56/122 (45.90%), Postives = 76/122 (62.30%), Query Frame = 0


Query: 88  SSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQ-K 147
           +SWC+   G S  VLQ  LDYACG  GADC+  +   SC+NP++VR H +YA NS++Q K
Sbjct: 19  ASWCVCKTGLSDTVLQATLDYACG-NGADCNPTKPKQSCFNPDNVRSHCNYAVNSFFQKK 78

Query: 148 NPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAPSGPSTSSS 207
              P SCNF GTA  T+++PS   C +P TS S S  +TT + G+T     P G  T+++
Sbjct: 79  GQSPGSCNFDGTATPTNSDPSYTGCAFP-TSASGSSGSTTVTPGTT----NPKGSPTTTT 134

Query: 208 AP 209
            P
Sbjct: 139 LP 134

[Close]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL
Match: A0A6J1FWZ7 (major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC111448235 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 6.2e-115
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 223
           SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 222

[Close]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL
Match: A0A6J1JFA6 (major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 PE=4 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.2e-110
Identity = 215/223 (96.41%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHC FPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCFFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATT TGGSSWCIA+LGASQA LQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKY-SSSALFLLLIAF 223
           S+LNTTNSSGSTVFGA PSGPSTSSSAPKY SSSALFLLLIAF
Sbjct: 181 SVLNTTNSSGSTVFGAVPSGPSTSSSAPKYSSSSALFLLLIAF 223

[Close]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL
Match: A0A1S4E1R5 (PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC103496317 PE=4 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.6e-86
Identity = 187/235 (79.57%), Postives = 198/235 (84.26%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGK-YRAA---SSTHQKDITTPITTVP 60
           MDG F  +TLI  LFLFIHC  PGSSRADP QKQGK +RAA   SST QKDITTPITTVP
Sbjct: 1   MDGRFIYSTLI--LFLFIHCFSPGSSRADPPQKQGKQFRAALRKSSTSQKDITTPITTVP 60

Query: 61  TINI-------PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYA 120
           TINI       PT+SNPD+VSP +TTPSFTPATT TGGSSWCIAS  ASQA LQLALDYA
Sbjct: 61  TINIPTIPIINPTSSNPDTVSPAMTTPSFTPATTITGGSSWCIASQSASQAALQLALDYA 120

Query: 121 CGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG 180
           CGMGGADCS+IQ GG+CYNPNSVRDHASYAFN+YYQKNPLPNSCNFGGTAVITSTNPSSG
Sbjct: 121 CGMGGADCSSIQAGGNCYNPNSVRDHASYAFNNYYQKNPLPNSCNFGGTAVITSTNPSSG 180

Query: 181 SCEYPSTSTSSSILNTTNSSGSTVFGAAPSGPST--SSSAPKYSSSALFLLLIAF 223
           +CEYPSTSTSSSILNTTNSSGSTVFGA PSGPST  SSSA  + S+  F LL  F
Sbjct: 181 TCEYPSTSTSSSILNTTNSSGSTVFGAVPSGPSTSSSSSALNFPSTQFFSLLTNF 233

[Close]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL
Match: A0A6J1D8X8 (glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111018000 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 8.4e-72
Identity = 158/211 (74.88%), Postives = 169/211 (80.09%), Query Frame = 0


Query: 2   DGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINIP 61
           +  FTCTTLI  L LF HC+ PGSS +   Q Q          QKDITTPITTVPTI+IP
Sbjct: 49  NASFTCTTLI--LLLFTHCISPGSSTSSIRQSQ-------KAGQKDITTPITTVPTISIP 108

Query: 62  T------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGA 121
           T      TS PD+VSP + TPSF PATTA GGSSWCIASLGASQA LQLALDYACG+GGA
Sbjct: 109 TMPIVNPTSTPDTVSPAMATPSFGPATTA-GGSSWCIASLGASQAALQLALDYACGVGGA 168

Query: 122 DCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPS 181
           DC  IQ GG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG+CEYPS
Sbjct: 169 DCGPIQGGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGTCEYPS 228

Query: 182 TSTSSSILNTTNSSGSTVFGAAPSGPSTSSS 207
           TSTSS++LNTTNSSGSTVFGA P+G   SSS
Sbjct: 229 TSTSSTVLNTTNSSGSTVFGAVPAGRGPSSS 249

[Close]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL
Match: A0A0A0KDA3 (X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 4.2e-71
Identity = 150/187 (80.21%), Postives = 162/187 (86.63%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGK-YRAA---SSTHQKDITTPITTVP 60
           MDG F C+TLI  LFLFIH   PGSSRADPTQKQGK +RAA   SST QKDITTPITTVP
Sbjct: 1   MDGRFLCSTLI--LFLFIHWFCPGSSRADPTQKQGKQFRAASRKSSTTQKDITTPITTVP 60

Query: 61  TINIPT-------TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYA 120
           TINIPT       +SNPD+VSP +TTPSFTP+TT +GGSSWCIAS  ASQA LQLALDYA
Sbjct: 61  TINIPTIPIINPASSNPDTVSPAMTTPSFTPSTTISGGSSWCIASQSASQAALQLALDYA 120

Query: 121 CGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSG 177
           CG+GGADCS+IQ GG+CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS+G
Sbjct: 121 CGIGGADCSSIQGGGNCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSTG 180

[Close]

BLAST of CmoCh20G002830 vs. NCBI nr
Match: XP_022943478.1 (major pollen allergen Ole e 10-like [Cucurbita moschata])

HSP 1 Score: 423.3 bits (1087), Expect = 1.3e-114
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 223
           SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 222

[Close]

BLAST of CmoCh20G002830 vs. NCBI nr
Match: XP_023513115.1 (major pollen allergen Ole e 10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 419.9 bits (1078), Expect = 1.4e-113
Identity = 220/222 (99.10%), Postives = 221/222 (99.55%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSS ADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSSADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSALFLLLIAF 223
           SILNTTNSSGSTVFGA+PSGPSTSSSAPKYSSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSALFLLLIAF 222

[Close]

BLAST of CmoCh20G002830 vs. NCBI nr
Match: KAG7010427.1 (Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 414.1 bits (1063), Expect = 7.7e-112
Identity = 220/228 (96.49%), Postives = 221/228 (96.93%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRA SSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAGSSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKY------SSSALFLLLIAF 223
           SILNTTNSSGSTVFGA+PSGPSTSSSAPKY      SSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSAPKYSSSSSSSSSALFLLLIAF 228

[Close]

BLAST of CmoCh20G002830 vs. NCBI nr
Match: KAG6570572.1 (Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 413.7 bits (1062), Expect = 1.0e-111
Identity = 219/224 (97.77%), Postives = 220/224 (98.21%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRA SSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAGSSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKY--SSSALFLLLIAF 223
           SILNTTNSSGSTVFGA+PSGPSTSSS PKY  SSSALFLLLIAF
Sbjct: 181 SILNTTNSSGSTVFGASPSGPSTSSSEPKYSSSSSALFLLLIAF 224

[Close]

BLAST of CmoCh20G002830 vs. NCBI nr
Match: XP_022986028.1 (major pollen allergen Ole e 10-like [Cucurbita maxima])

HSP 1 Score: 409.1 bits (1050), Expect = 2.5e-110
Identity = 215/223 (96.41%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60
           MDGGFTCTTLILFLFLFIHC FPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI
Sbjct: 1   MDGGFTCTTLILFLFLFIHCFFPGSSRADPTQKQGKYRAASSTHQKDITTPITTVPTINI 60

Query: 61  PTTSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAI 120
           PTTSNPDSVSPTITTPSFTPATT TGGSSWCIA+LGASQA LQLALDYACGMGGADCSAI
Sbjct: 61  PTTSNPDSVSPTITTPSFTPATTTTGGSSWCIANLGASQAALQLALDYACGMGGADCSAI 120

Query: 121 QTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180
           QTGG CYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS
Sbjct: 121 QTGGRCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSS 180

Query: 181 SILNTTNSSGSTVFGAAPSGPSTSSSAPKY-SSSALFLLLIAF 223
           S+LNTTNSSGSTVFGA PSGPSTSSSAPKY SSSALFLLLIAF
Sbjct: 181 SVLNTTNSSGSTVFGAVPSGPSTSSSAPKYSSSSALFLLLIAF 223

[Close]

BLAST of CmoCh20G002830 vs. TAIR 10
Match: AT2G30933.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.2e-41
Identity = 111/232 (47.84%), Postives = 136/232 (58.62%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSR-ADPTQKQGKYRAASSTHQKDITTPITTVPTIN 60
           MD GF   T +L L  F    F G+    +P ++           +KDITTP+ T PT  
Sbjct: 1   MDKGFRLVTSLLLLSFF----FSGTKAITEPIEE-----------EKDITTPLATNPT-T 60

Query: 61  IPTTSNPDSVSPT-------ITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGM 120
            PTT  P+S S         +T PS        G  SWC+A    ++  LQ ALDYACG+
Sbjct: 61  TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGI 120

Query: 121 GGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCE 180
           GGADCS IQ GG+CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS GSC 
Sbjct: 121 GGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPSLGSCH 180

Query: 181 YPSTSTSSSILNTTNSSGSTVFGAAPSGPSTSSSAPKYSSSAL--FLLLIAF 223
           +PSTSTS SILN T+  G  +FG  PS P+    A   SS  L  FL  + F
Sbjct: 181 FPSTSTSESILNVTSEDGLGLFGRIPSHPTPKPEASTSSSRTLISFLYFLCF 216

[Close]

BLAST of CmoCh20G002830 vs. TAIR 10
Match: AT2G30933.2 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 135.2 bits (339), Expect = 6.5e-32
Identity = 84/175 (48.00%), Postives = 104/175 (59.43%), Query Frame = 0


Query: 1   MDGGFTCTTLILFLFLFIHCLFPGSSR-ADPTQKQGKYRAASSTHQKDITTPITTVPTIN 60
           MD GF   T +L L  F    F G+    +P ++           +KDITTP+ T PT  
Sbjct: 1   MDKGFRLVTSLLLLSFF----FSGTKAITEPIEE-----------EKDITTPLATNPT-T 60

Query: 61  IPTTSNPDSVSPT-------ITTPSFTPATTATGGSSWCIASLGASQAVLQLALDYACGM 120
            PTT  P+S S         +T PS        G  SWC+A    ++  LQ ALDYACG+
Sbjct: 61  TPTTVVPNSDSDASAVATTPLTIPSSPHGVAYPGDQSWCVARENVAKMALQAALDYACGI 120

Query: 121 GGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNPS 168
           GGADCS IQ GG+CYNPNS+R HAS+AFNSYYQKNP+P+SCNF GTA+  S +PS
Sbjct: 121 GGADCSEIQEGGNCYNPNSLRAHASFAFNSYYQKNPIPSSCNFDGTAITISADPS 159

[Close]

BLAST of CmoCh20G002830 vs. TAIR 10
Match: AT1G29380.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.5e-28
Identity = 67/134 (50.00%), Postives = 81/134 (60.45%), Query Frame = 0


Query: 79  TPATTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASY 138
           T A    G   WCIA   AS   LQ+ALDYACG GGADC  IQ G +CY PN++RDHAS+
Sbjct: 137 TGAGGGVGSGQWCIAKANASPTSLQVALDYACGYGGADCGQIQQGAACYEPNTIRDHASF 196

Query: 139 AFNSYYQKNPLPNSCNFGGTAVITSTNPSSGSCEYPSTSTSSSILNTTNSSGSTVFGAAP 198
           AFNSYYQK+P  +SCNFGG A +TST+PS GSC + S+S                 G   
Sbjct: 197 AFNSYYQKHPGSDSCNFGGAAQLTSTDPSKGSCHFSSSS-----------------GTVS 253

Query: 199 SGPSTSSSAPKYSS 213
           + P +  S P +SS
Sbjct: 257 TSPPSQMSPPDFSS 253

[Close]

BLAST of CmoCh20G002830 vs. TAIR 10
Match: AT1G09460.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-25
Identity = 75/159 (47.17%), Postives = 98/159 (61.64%), Query Frame = 0


Query: 48  ITTPITTVPTINIPT-TSNPDSVSPTITTPSFTPATTATGGSSWCIASLGASQAVLQLAL 107
           +T P+T   T   PT  S    V   +  P     + +  G SWC+A  GASQ  LQ AL
Sbjct: 94  VTNPVTNPVTQYPPTQPSGTVPVPVPVVAPPVVSNSPSVSGQSWCVAKPGASQVSLQQAL 153

Query: 108 DYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFNSYYQKNPLPNSCNFGGTAVITSTNP 167
           DYACG+  ADCS +Q GG+CY+P S++ HAS+AFNSYYQKNP P SC+FGG A + +TNP
Sbjct: 154 DYACGI--ADCSQLQQGGNCYSPISLQSHASFAFNSYYQKNPSPQSCDFGGAASLVNTNP 213

Query: 168 SSGSCEYPSTSTSSSIL--NTTNSSGSTVFGAAPSGPST 204
           S+GSC Y + S++S+ +   TT  + ST     P   ST
Sbjct: 214 STGSCIYQTGSSTSTPMTAGTTTPTPSTQTVNQPPVTST 250

[Close]

BLAST of CmoCh20G002830 vs. TAIR 10
Match: AT4G05430.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 8.8e-21
Identity = 51/101 (50.50%), Postives = 67/101 (66.34%), Query Frame = 0


Query: 82  TTATGGSSWCIASLGASQAVLQLALDYACGMGGADCSAIQTGGSCYNPNSVRDHASYAFN 141
           TT   G++WC+A  GA+QA LQ ALD+ACG+G  DCS I+  G CY P+++  HAS+AFN
Sbjct: 15  TTFLEGTTWCVARPGATQAELQRALDWACGIGRVDCSVIERHGDCYEPDTIVSHASFAFN 74

Query: 142 SYYQKNPLPN-SCNFGGTAVITSTNPSSGSCEYPSTSTSSS 182
           +YYQ N     +C FGGTA  T  NPS G C Y ++ +  S
Sbjct: 75  AYYQTNGNNRIACYFGGTATFTKINPSYGKCSYDASKSEVS 115

The following BLAST results are available for this feature:

BLAST of CmoCh20G002830 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84V39	3.0e-18	43.65	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
O65399	4.0e-18	47.41	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q9ZU91	4.0e-18	43.75	Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ...	[more]
Q9SD84	5.2e-18	45.83	PLASMODESMATA CALLOSE-BINDING PROTEIN 2 OS=Arabidopsis thaliana OX=3702 GN=PDCB2...	[more]
Q9FNQ2	3.4e-17	45.90	PLASMODESMATA CALLOSE-BINDING PROTEIN 1 OS=Arabidopsis thaliana OX=3702 GN=PDCB1...	[more]

BLAST of CmoCh20G002830 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWZ7	6.2e-115	100.00	major pollen allergen Ole e 10-like OS=Cucurbita moschata OX=3662 GN=LOC11144823...	[more]
A0A6J1JFA6	1.2e-110	96.41	major pollen allergen Ole e 10-like OS=Cucurbita maxima OX=3661 GN=LOC111483888 ...	[more]
A0A1S4E1R5	4.6e-86	79.57	PLASMODESMATA CALLOSE-BINDING PROTEIN 5-like OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1D8X8	8.4e-72	74.88	glucan endo-1,3-beta-glucosidase 3-like OS=Momordica charantia OX=3673 GN=LOC111...	[more]
A0A0A0KDA3	4.2e-71	80.21	X8 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139780 PE=4 SV=...	[more]

BLAST of CmoCh20G002830 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943478.1	1.3e-114	100.00	major pollen allergen Ole e 10-like [Cucurbita moschata]	[more]
XP_023513115.1	1.4e-113	99.10	major pollen allergen Ole e 10 [Cucurbita pepo subsp. pepo]	[more]
KAG7010427.1	7.7e-112	96.49	Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6570572.1	1.0e-111	97.77	Glucan endo-1,3-beta-glucosidase 1, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022986028.1	2.5e-110	96.41	major pollen allergen Ole e 10-like [Cucurbita maxima]	[more]

BLAST of CmoCh20G002830 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G30933.1	1.2e-41	47.84	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G30933.2	6.5e-32	48.00	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G29380.1	2.5e-28	50.00	Carbohydrate-binding X8 domain superfamily protein	[more]
AT1G09460.1	1.2e-25	47.17	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G05430.1	8.8e-21	50.50	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 89..173 e-value: 7.3E-45 score: 165.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 90..159 e-value: 4.6E-20 score: 72.1
None	No IPR available	GENE3D	1.20.58.1040		coord: 84..191 e-value: 2.5E-11 score: 45.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 51..71
None	No IPR available	PANTHER	PTHR31044:SF86	CARBOHYDRATE-BINDING X8 DOMAIN SUPERFAMILY PROTEIN	coord: 16..210
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 16..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh20G002830.1	CmoCh20G002830.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016020	membrane
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds