CmoCh19G007360 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh19G007360
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionGlutamyl-tRNA(Gln) amidotransferase subunit A
LocationCmo_Chr19: 7586416 .. 7590305 (+)
RNA-Seq ExpressionCmoCh19G007360
SyntenyCmoCh19G007360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCCACGTTCGGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACAGTACCTGACCTCCAGCTAGCTTTCAAGCGAAACGAGCTCACTTCGAGGGGCCTTGTTGAGTTCTACATCGGAGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGGCTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTATTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTGCCCCGTGACGCAGGTGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCTCTTGCTGCTCCTGCTGGTTTGAGCGCTAGAGGTGGCCAGGGAAAGGTGTGTTGCTTTGAATTTAGGTTCCTACTTAACTAAAAACCTATAGCAACTCAAGTCACGGCGAGTCGATATTATCCTCTTTAGGTTTTCCCTTTCAGGCTTCCTCTCGAGGGTTTAAAATGTGTCCACTAGGGAGAGGTTTTCACACTCTTATAAGGAATGTTTCGTTTTGTTCCCCTCTCCAACCGATGTGTGATCTCACAATTCACCCCTTCGGAGACCCAACGTCCTCGTTGGCACAGCCCCTGGTGTCTAGCTCTGACCATTTGTAACAATCCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGACTTTTTCCTTCCGGGCTTCCCCTCAAGGTTTTAAAACATGTCCACTAGGGAGAGGTTTCCACACTTTTATATAAGGAATGTTTCGTTTTGTTCCCCTCTCCAACCGATGTGGGATCTCACAATCCACCCCTTAGGAGGCCAGCATTCTCGTTGGCACACCGCCTAGTGTCTATCTCTGACCATTTGTAACAGTCCAAGCCCATTGCTAGTAGATATTGTCCTCTTTAGACTTCCCCTTGAAGTTTTAAAACGCGTCCACTAGGAAGAGGTTTCCACACCCTTATAAGTAATGTTTCGTTTTGTTCCCCTCTCCAACCGATGTGGGATATCACAATCCACTCCCCTTGAGGACCCAGCGTCCTCGTTAGCACACCGCCCAGTGTCTGGCTCTGATACCATTTATAACAGCCCAAACTCACCGCTAGCAGACATTTTCCTCTCTAGGCTTTCCCTTTTCGGCTTCCCCTCAATGGTTTTAAATCGCGTCCACTAGGGAGACGTTTCCACACCCTTAAAAGCATTGTTTCGTTCTTCTCTCTAACCGATATGGGATCTCACAAAACCATTTCTTTTGTACAATTTTTTTTGCAGAATCCATATGTTTTATCAGCATCACCCTGTGGATCCAGCAGTGGACCTTCCATATCAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGCTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCAACGGTCGGCCTCACAAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGTGAGCAATCTGGCATCCATATCAGCAAAATATTCTACACAGATCATAAGAACTGAAACTTTGTAACCTTCACATGTGAAAACAGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTTTCAGCTTCTTCAATGATTCAGCCATCACTCAGGCTTTTGAGGATCATTTCAACATTCTAAAGTAAGAAAACCAACTTCCCTATCCATGCAAATGCTAATCATTTCATAAACAGGTTCATATTCACTTTGGGTTCATATTCACTTAAAACAAAAGGCTCGATCATTAATCTGAGATCACATACGGTTGGGGAGGAGAACAAAGCATTGGTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTGAAAGGAAAGTCTAAAAAGGACAATATCTGTTAGCGGTGAGCTTGAGCTGTTACAGATGATATCAGAACCAGACACCAGGCGGTGTGCCAGCGAGGACATTGGACTCCCAAGGGGGTGGATTGTGAGATCCCACATCGGTTTGAGAGGGGAACGAAACATTTCTTATAAGGGTGTAGAAACCTCTCCCTAGTAGACACGTTGGTGGATTGTGAGATCCCACATCAGTTGGAGAGGGGTACGAAACATTGCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTGAAGGGAATCTCGGAAGGGAAAGCCTAAAGAGGACAATATCTGCTATTAGTGGGCTTGGGCTGTTACAAATAGTATCAGAACCGAACACCAGGCGGTGTGCCAGCGAGGATGTTGGGCCCCAGGAGGGTGGATTGTGAGATTCCACACCGATTGGAGAAGAGAACAAAACATTGCTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACACGGTGGATTGTAAGATCTCACATCGGTTGAAGAGGGGAACGAAACATTGTTTATAATAATGTGAGAACCTCTCCCTAGTAGATTTACTTATAAGGGTATGAAAACATCTCCCTAGTGGACTTGATTATAAGGGTGTGAAAAACTCTCTCTAGTAGACGCGTTGGTGGATTATGAGATCCCATATTGGTTGGAGAGGGGAACAAAGCATTGCTTATAAGGGTGTGGAAACTCTTCCCTAGTAGACGTGTTTTAAAACCTTGAAGGAAAGCCTGAAACGAAGGGAAAACCAAAGAGGACAATATCAACTAGCAGTGAACTTGAACTCTTTCTTACGGATTAAGAAAAATCAATTCAACATGAACACAAACAACCAATCTTCAAAAGGAATAGTTTACAATCCTCTCAACTCAAATCATTTTCCATTGTTCTTCTAGTAAACACTAAATCTAACATTGTTGAAACTCAAGGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGAAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGTTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGTAACACAATATTCAATCCATTGTTCATCGCATAGTAATCGTATTCATTCGGAATCGATTCGAATCATGGTTGTGCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCTACAAACGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGATTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATTGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTTGGAGGGTTGAAGGGTTCAGAGCCGACGCTGATTGAGGTTGCTTATGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGAAGTGAATCAAATTACAACTTTCATCAATTGAGCTATGTGTTAAAAGATATTGAAATTAATTGACATTTTAGCTATTGAACTACAACATGAAACTACTTTGTGTTGCACTGATATAATTTAATTCGAACAAAATTCTTG

mRNA sequence

ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCCACGTTCGGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACAGTACCTGACCTCCAGCTAGCTTTCAAGCGAAACGAGCTCACTTCGAGGGGCCTTGTTGAGTTCTACATCGGAGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGGCTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTATTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTGCCCCGTGACGCAGGTGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCTCTTGCTGCTCCTGCTGGTTTGAGCGCTAGAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGCTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCAACGGTCGGCCTCACAAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTTTCAGCTTCTTCAATGATTCAGCCATCACTCAGGCTTTTGAGGATCATTTCAACATTCTAAAGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGAAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGTTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCTACAAACGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGATTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATTGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTTGGAGGGTTGAAGGGTTCAGAGCCGACGCTGATTGAGGTTGCTTATGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGAAGTGAATCAAATTACAACTTTCATCAATTGAGCTATGTGTTAAAAGATATTGAAATTAATTGACATTTTAGCTATTGAACTACAACATGAAACTACTTTGTGTTGCACTGATATAATTTAATTCGAACAAAATTCTTG

Coding sequence (CDS)

ATGGCCTACTCCTCCTGGCTTCTGATTATTGTGGCCTTTTGTTTGACGGTGCCACGTTCGGCAGCTGTTCGAGGATTTTCGATCAGAGAAGCCACAGTACCTGACCTCCAGCTAGCTTTCAAGCGAAACGAGCTCACTTCGAGGGGCCTTGTTGAGTTCTACATCGGAGAAATTCGCAGACTCAACCCGGTTGTCCATGGTGTCATAGAAATCAACCCAGATGCCTTGCTGCAAGCTTACAAGGCTGACAGAGAGCGTGAGGCTAAGAAGCCTGGATCGCTTTGTGGGCTGCATGGAATCCCAGTTTTATTGAAGGATAGCATTGGGACTAAGGATAAGTTGAACACTACTGCTGGGTCGTTCGCGTTGCTTGGCTCTATCGTGCCCCGTGACGCAGGTGTGGTGAAGAGACTAAGAAGGGCTGGAGCCATCATACTGGGAAAGGCTAGCTTGAGTGAATGGGCTGATTTTAGGTCTCTTGCTGCTCCTGCTGGTTTGAGCGCTAGAGTTGCAGCAAACATAGCAGCAGTGTCAATAGGAACTGAAACCGACGGCTCTATCCTCTGTCCAGCTAGTTTTAACTCGGTCGTCGGTATCAAACCAACGGTCGGCCTCACAAGTAGAGCAGGTGTCATTCCAGTCTCCCCAAGACAAGACACTATTGGGCCAATCGGCAGGACAGTGACGGATGCTGTAATAGTTCTTGACACCATTGTAGGATTTGATTACAATGATGAAGCCACCAGAACATCCTCCAAGTATATTCCATACGGTGGCTACAAACAATTCCTCAATGCAAATGGGTTGAAAGGGAAACGATTGGGCATTGTGAGGAACCCTTTTTTCAGCTTCTTCAATGATTCAGCCATCACTCAGGCTTTTGAGGATCATTTCAACATTCTAAAGCAAGGTGGTGCAATTTTGGTAGACAATCTAGAGATAGAAAATATAGATATCATCTTAAACGTGACTGCAAGTGGAGAAGCAGTGGCATTGTTAGCTGAATTCAAACAATCATTAAATGAGTACCTGAAAGAGCTGGTGGCTTCCCCTGTTCGAAGTTTGGCCGACATAATTGCCTTCGATAACGCAAATCCAGATCAGGAACTGCTCGACGTTTTCGGCCAGGAGATTTTTCTCGCGGCCGAAGCTACAAACGGGATCGGCGACGTGCAGAAGGCGGCTCTGTTGAACTTGGCGAAGCTGACGAAGGATGGGTTTGAGAAAGTGGTGGAGGAGGAGCGATTGGATGCGGTGGTGACGCCAGGTCCCGCCATAGCTACGGTGCTTGCAATTGGGGGATTTCCGGGAATCAATGTTCCGGCGGGATACGACGGCGGAGGAGTTCCATTTGGGATTAACTTTGGAGGGTTGAAGGGTTCAGAGCCGACGCTGATTGAGGTTGCTTATGCGTTCGAGCAGGCCACTCTGATAAGAAAGCCTCCTTCCTTCAAGCCTTGA

Protein sequence

MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPTLIEVAYAFEQATLIRKPPSFKP
Homology
BLAST of CmoCh19G007360 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 7.3e-158
Identity = 300/512 (58.59%), Postives = 381/512 (74.41%), Query Frame = 0

Query: 3   YSSWLLIIV---AFCLTVPRSAAVR---GFSIREATVPDLQLAFKRNELTSRGLVEFYIG 62
           +SS L++++   A  ++V  ++ +R    FSI+EAT+ D+++AF    LTS+ LVE Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 63  EIRRLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNT 122
            I +LNP++H VIE NPDAL+QA  ADRER+ K    L  LHG+PVLLKDSI TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 123 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR------- 182
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASLSEWA FRS + P G SAR       
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNP 188

Query: 183 -----------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVI 242
                            V AN+ AVS+GTETDGSIL PAS NSVVGIKP+VGLTSRAGV+
Sbjct: 189 YVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVV 248

Query: 243 PVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGK 302
           P+S RQD+IGPI RTV+DAV +LD IVG+D  DEAT+T+S++IP GGYKQFL  +GLKGK
Sbjct: 249 PISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGK 308

Query: 303 RLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVAL 362
           RLGIV             +   + H   L++ GAI+++NL I NI++I+  T SGE +AL
Sbjct: 309 RLGIVMKH----------SSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIAL 368

Query: 363 LAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQ 422
           LAEFK SLN YLKELV SPVRSLAD+IA++    +QE +  +GQE+FL AEAT+G+G+ +
Sbjct: 369 LAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKE 428

Query: 423 KAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFG 482
           K AL  + +L+++G EK++EE +LDA+VT G  +++VLAIGG+PGINVPAGYD GGVP+G
Sbjct: 429 KTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYG 488

Query: 483 INFGGLKGSEPTLIEVAYAFEQATLIRKPPSF 485
           I+FGGL+ SEP LIE+A+AFEQATLIRKPP F
Sbjct: 489 ISFGGLRFSEPKLIEIAFAFEQATLIRKPPKF 510

BLAST of CmoCh19G007360 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.0e-67
Identity = 184/515 (35.73%), Postives = 261/515 (50.68%), Query Frame = 0

Query: 24  RGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKAD 83
           +  ++ +AT+  LQ   +   LTS  +V  Y+    ++NP V+G++++NPD L  A + D
Sbjct: 62  QNITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD 121

Query: 84  REREAKKPGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAG 143
            ER     G + G LHGIP ++KD+  TKDK++TTAGS+ALLGSIVPRDA VVK+LR AG
Sbjct: 122 DER---ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 181

Query: 144 AIILGKASLSEWADFRSLAAPAGLSAR------------------------VAANIAAVS 203
           A++ G A+LSEWAD RS     G SAR                        VA+N+ A +
Sbjct: 182 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 241

Query: 204 IGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTI 263
           +GTETDGSI+ PA  N VVG+KPTVGLTSR GVIP S  QDT GPI RTV DAV V  ++
Sbjct: 242 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 301

Query: 264 VGFDYNDEATRTSSKYIPY-GGYKQFL-NANGLKGKRLGIVRNPFFSFFNDSAITQAFED 323
            G D ND  T   +   P  G Y +FL N   L+G R G+     +       I +  E 
Sbjct: 302 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLE- 361

Query: 324 HFNILKQGGAILVDNLEIENIDII--------LNVTASGEAVALLAEFKQSLNEYLKELV 383
               +++ GAI+ +N    N+D+I        L      E   +  +F  ++  YL E+ 
Sbjct: 362 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 421

Query: 384 ASPVRSLADIIAFDNANPDQE-----LLDVF--GQEIFLAAEATNGIGDVQKAALLNLAK 443
            + + SL DI+ ++N     E     ++  F  GQ+ FLA+    G+ +      +   +
Sbjct: 422 NTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVR 481

Query: 444 LTK--DGFE--------KVVEEERLDAVVTPGPAIATV--LAIGGFPGINVPAGYDGGGV 485
            T   +G +        K  +   L+ ++ P     T    A  G+P I +P G    G 
Sbjct: 482 RTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 541

BLAST of CmoCh19G007360 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 1.0e-58
Identity = 167/491 (34.01%), Postives = 243/491 (49.49%), Query Frame = 0

Query: 36  LQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREAKKPGSLC 95
           LQ  +++  +    +V+ Y+  I  +N  V  V EINPDAL  A + D ER   K G L 
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNER---KMGKLR 153

Query: 96  G-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEW 155
           G LHG+P+++K++I T DK+++TAGS+A+ G+    DA V  +LR AG +I+GK+  S+W
Sbjct: 154 GPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 156 ADFRSLAAPAGLSA---RVAA---------------------NIAAVSIGTETDGSILCP 215
           A+FRSL +  G SA   +V A                      +A  ++GTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 216 ASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRT 275
           A  +++VG+KPTVGLTSR  V+P+S RQDT+GP+ R+V DA  +L  I G D ND  T  
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSA 333

Query: 276 SSKYIPYGGYKQFLNA---NGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAI 335
               IP+     ++ A   N LKGKR+G+ RN    F +   +   F     ++K+ GAI
Sbjct: 334 ----IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAI 393

Query: 336 LVDNLEIENIDIILNVTASGEAVALLAEFKQSLNEYLKELVASP--VRSLADIIAFDNAN 395
           +V+N +  +           +   L A+   +L  + K+L  +P  +  L  +  F   +
Sbjct: 394 IVENTDFTSFAEFAQSPIPDD--ILYADSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH 453

Query: 396 PDQEL--LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPG 455
             +E    D    +I L     N           N+    + G    +   +LDA V P 
Sbjct: 454 RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLPT 513

Query: 456 PAIATVLAIGGFPGINVPAGY---------------DGGGVPFGINFGGLKGSEPTLIEV 480
                + A+ G P I VP G                 G G+P GI F G   SE  LI +
Sbjct: 514 DLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPIGIGFMGDLWSEEKLIGL 573

BLAST of CmoCh19G007360 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 4.3e-41
Identity = 151/499 (30.26%), Postives = 237/499 (47.49%), Query Frame = 0

Query: 32  TVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREAKKP 91
           T+ +L+   K+ E+++  + + Y+  I+ + P +  ++ I  D  LQ  KA    E  K 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQ--KAKEADEKIKK 66

Query: 92  GSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASL 151
           G    L GIPV++KD+I T + + TT  S  L   I P +A VV++L   G IILGK++L
Sbjct: 67  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 126

Query: 152 SEWA--------DFRSLAAP-----------AGLSARVAANIAAVSIGTETDGSILCPAS 211
            E+A         F++   P            G +A +AA+ AA ++G++T GSI  PAS
Sbjct: 127 DEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQPAS 186

Query: 212 FNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSS 271
              VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G D  D    ++S
Sbjct: 187 LCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKD----STS 246

Query: 272 KYIPYGGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNL 331
             I    Y  +L  + +KG R+G+ +  FF    +  + +  ++   +L+  GA ++D +
Sbjct: 247 LKIDKPDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVLQDLGAEIID-I 306

Query: 332 EIENIDIIL---NVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQE 391
            I  ++  L    + AS EA + LA +      +    +A     L D+     +     
Sbjct: 307 SIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAEKYEDLIDMYMVTRS----- 366

Query: 392 LLDVFGQE----IFLAAEA-TNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTP-G 451
             + FG+E    I L   A ++G  D      L +  L K+ FEK    E+ D ++ P  
Sbjct: 367 --EGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPTS 426

Query: 452 PAIATVLA--------------------IGGFPGINVPAGYDGGGVPFGINFGGLKGSEP 483
           P +A  +                     I G PGI++P G    G+P G+   G    E 
Sbjct: 427 PTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQIIGRHFDEG 481

BLAST of CmoCh19G007360 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 7.3e-41
Identity = 151/499 (30.26%), Postives = 237/499 (47.49%), Query Frame = 0

Query: 32  TVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREAKKP 91
           T+ +L+   K+ E+++  + + Y+  I+ + P +  +I I  D  LQ  +A    E  K 
Sbjct: 7   TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQ--RAKEADEKIKN 66

Query: 92  GSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASL 151
           G    L GIPV++KD+I T + + TT  S  L   I P +A VV++L   G IILGK++L
Sbjct: 67  GEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNL 126

Query: 152 SEWA--------DFRSLAAP-----------AGLSARVAANIAAVSIGTETDGSILCPAS 211
            E+A         F++   P            G +A +AA+ AA ++G++T GSI  PAS
Sbjct: 127 DEFAMGSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQPAS 186

Query: 212 FNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSS 271
              VVG+KPT GL SR G++  +   D IGP  + VTD  IVL+TI+G D  D    ++S
Sbjct: 187 LCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKD----STS 246

Query: 272 KYIPYGGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNL 331
             I    Y  +L  + +KG R+G+ +  FF    +  + +  ++   +L+  GA ++D +
Sbjct: 247 LKIDKPDYTSYLKED-IKGLRIGVAKE-FFGEGIEEGVKETVQESIKVLQDLGAEIID-I 306

Query: 332 EIENIDIIL---NVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQE 391
            I  ++  L    + AS EA + LA +      +    +A     L D+     +     
Sbjct: 307 SIPYVEYALPAYYIIASAEASSNLARYDGIRYGH----IAGKYEDLIDMYMVTRS----- 366

Query: 392 LLDVFGQE----IFLAAEA-TNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTP-G 451
             + FG+E    I L   A ++G  D      L +  L K+ FEK    E+ D ++ P  
Sbjct: 367 --EGFGKEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPTS 426

Query: 452 PAIATVLA--------------------IGGFPGINVPAGYDGGGVPFGINFGGLKGSEP 483
           P +A  +                     I G PGI++P G    G+P G+   G    E 
Sbjct: 427 PTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGL-SDGLPVGLQIIGRHFDEG 481

BLAST of CmoCh19G007360 vs. ExPASy TrEMBL
Match: A0A6J1GLV2 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.1e-265
Identity = 486/510 (95.29%), Postives = 486/510 (95.29%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEPTLIEVAYAFEQATLIRKPPSFKP
Sbjct: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 510

BLAST of CmoCh19G007360 vs. ExPASy TrEMBL
Match: A0A6J1HUS8 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.1e-261
Identity = 479/510 (93.92%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MA SSWLLIIVAFCLTVPRSAAVRGFSIREATVPDL LAFKRNELTSRGLVEFYIGEIRR
Sbjct: 17  MACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND ATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGA+LVDNLEI NIDIILNVTASGEA ALLAEF
Sbjct: 317 VRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 436

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 437 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 496

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEP LIEVAYAFEQATLIRKPPSFKP
Sbjct: 497 GLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

BLAST of CmoCh19G007360 vs. ExPASy TrEMBL
Match: A0A0A0K2I0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.7e-238
Identity = 439/506 (86.76%), Postives = 454/506 (89.72%), Query Frame = 0

Query: 5   SWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPV 64
           S LLI+VAFC T   S  VRG SIREATV DLQLAFK+N+LTSR LV FYIGEIRRLNPV
Sbjct: 13  SLLLILVAFCSTPTHSVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDALLQAYKADREREA KPGSLCGLHGIPVLLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALLQAYKADREREANKPGSLCGLHGIPVLLKDNIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR--------------- 184
           GSIVPRDAGVVKRLR+AGAIILGKASLSEWADFRSLAAPAGLSAR               
Sbjct: 133 GSIVPRDAGVVKRLRKAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
                    VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSL 364
           FFSFFNDS ITQAFEDHFN LKQGGAIL+DNLEI +IDIILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFEDHFNTLKQGGAILIDNLEIADIDIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAA+LNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAAVLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 484
           KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKENQLDAVVTPGTGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

Query: 485 SEPTLIEVAYAFEQATLIRKPPSFKP 487
           SE  LIEVAY FEQATLIRKPPSFKP
Sbjct: 493 SETKLIEVAYGFEQATLIRKPPSFKP 518

BLAST of CmoCh19G007360 vs. ExPASy TrEMBL
Match: A0A1S4DXK2 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 9.7e-238
Identity = 435/506 (85.97%), Postives = 453/506 (89.53%), Query Frame = 0

Query: 5   SWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPV 64
           S LLI+VAFCLT P    VRG SIREATV DLQLAFK+N+LTSR LV FYIGEIRRLNPV
Sbjct: 13  SMLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDAL+QAYKADREREA KPGSLCGLHGIP+LLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR--------------- 184
           GSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSL APAGLSAR               
Sbjct: 133 GSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
                    VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSL 364
           FFSFFNDS ITQAF+DHFN LKQGGAIL+DNLEI NIDIILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANIDIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAALLNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 484
           KLT+DGFEK+V++ +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKDNQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

Query: 485 SEPTLIEVAYAFEQATLIRKPPSFKP 487
           SE  LIEVAY FEQAT IRKPPSFKP
Sbjct: 493 SEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CmoCh19G007360 vs. ExPASy TrEMBL
Match: A0A5A7U6F5 (Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00780 PE=4 SV=1)

HSP 1 Score: 831.2 bits (2146), Expect = 2.2e-237
Identity = 435/506 (85.97%), Postives = 453/506 (89.53%), Query Frame = 0

Query: 5   SWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPV 64
           S LLI+VAFCLT P    VRG SIREATV DLQLAFK+N+LTSR LV FYIGEIRRLNPV
Sbjct: 13  SLLLILVAFCLTTPYCVTVRGLSIREATVHDLQLAFKQNQLTSRELVNFYIGEIRRLNPV 72

Query: 65  VHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALL 124
           VHGVIEINPDAL+QAYKADREREA KPGSLCGLHGIP+LLKD+IGTKDKLNTTAGSFALL
Sbjct: 73  VHGVIEINPDALMQAYKADREREANKPGSLCGLHGIPILLKDTIGTKDKLNTTAGSFALL 132

Query: 125 GSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR--------------- 184
           GSIVPRDAG+VKRLR+AGAIILGKASLSEWADFRSL APAGLSAR               
Sbjct: 133 GSIVPRDAGIVKRLRKAGAIILGKASLSEWADFRSLTAPAGLSARGGQGKNPYVLSASPC 192

Query: 185 ---------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 244
                    VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT
Sbjct: 193 GSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 252

Query: 245 IGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNP 304
           IGPIGRTVTDAVIVLDTIVGFDYND ATRT+SKYIPYGGYKQFLN NGLKGKRLGIVRNP
Sbjct: 253 IGPIGRTVTDAVIVLDTIVGFDYNDAATRTASKYIPYGGYKQFLNPNGLKGKRLGIVRNP 312

Query: 305 FFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSL 364
           FFSFFNDS ITQAF+DHFN LKQGGAIL+DNLEI NI+IILNVTASGEA ALLAEFKQSL
Sbjct: 313 FFSFFNDSTITQAFDDHFNTLKQGGAILIDNLEIANINIILNVTASGEAAALLAEFKQSL 372

Query: 365 NEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLA 424
           N YLKELV SPVRSLADIIAF+NAN DQELL+VFGQEIFLAAEATNGIGDVQKAALLNL 
Sbjct: 373 NGYLKELVVSPVRSLADIIAFNNANADQELLNVFGQEIFLAAEATNGIGDVQKAALLNLG 432

Query: 425 KLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 484
           KLT+DGFEK+V+E +LDAVVTPG  IATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG
Sbjct: 433 KLTEDGFEKLVKENQLDAVVTPGSGIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKG 492

Query: 485 SEPTLIEVAYAFEQATLIRKPPSFKP 487
           SE  LIEVAY FEQAT IRKPPSFKP
Sbjct: 493 SEAKLIEVAYGFEQATQIRKPPSFKP 518

BLAST of CmoCh19G007360 vs. NCBI nr
Match: XP_022953021.1 (putative amidase C869.01 [Cucurbita moschata])

HSP 1 Score: 925.2 bits (2390), Expect = 2.3e-265
Identity = 486/510 (95.29%), Postives = 486/510 (95.29%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEPTLIEVAYAFEQATLIRKPPSFKP
Sbjct: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 510

BLAST of CmoCh19G007360 vs. NCBI nr
Match: KAG7011753.1 (hypothetical protein SDJN02_26659, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 919.5 bits (2375), Expect = 1.2e-263
Identity = 484/510 (94.90%), Postives = 484/510 (94.90%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR
Sbjct: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 180

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 181 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFD NDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDCNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEI NIDIILNVTASGEAVALLAEF
Sbjct: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 360

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEPTLIEVAYAFEQATLIRKPPSFKP
Sbjct: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 510

BLAST of CmoCh19G007360 vs. NCBI nr
Match: KAG6572101.1 (putative amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916.4 bits (2367), Expect = 1.1e-262
Identity = 483/510 (94.71%), Postives = 483/510 (94.71%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYI EIRR
Sbjct: 71  MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIREIRR 130

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 131 LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 190

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 191 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 250

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 251 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 310

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFD NDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 311 RQDTIGPIGRTVTDAVIVLDTIVGFDCNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 370

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEI NIDIILNVTASGEAVALLAEF
Sbjct: 371 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 430

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 431 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 490

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 491 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 550

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEPTLIEVAYAFEQATLIRKPPSFKP
Sbjct: 551 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 580

BLAST of CmoCh19G007360 vs. NCBI nr
Match: XP_022968892.1 (putative amidase C869.01 [Cucurbita maxima])

HSP 1 Score: 911.0 bits (2353), Expect = 4.4e-261
Identity = 479/510 (93.92%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MA SSWLLIIVAFCLTVPRSAAVRGFSIREATVPDL LAFKRNELTSRGLVEFYIGEIRR
Sbjct: 17  MACSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLHLAFKRNELTSRGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYND ATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDAATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPFFSFFNDSAITQAFEDHFNILKQGGA+LVDNLEI NIDIILNVTASGEA ALLAEF
Sbjct: 317 VRNPFFSFFNDSAITQAFEDHFNILKQGGAVLVDNLEIANIDIILNVTASGEAAALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAAL 436

Query: 421 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 480
           LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG
Sbjct: 437 LNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFG 496

Query: 481 GLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           GLKGSEP LIEVAYAFEQATLIRKPPSFKP
Sbjct: 497 GLKGSEPKLIEVAYAFEQATLIRKPPSFKP 526

BLAST of CmoCh19G007360 vs. NCBI nr
Match: XP_023554278.1 (probable amidase At4g34880 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 894.4 bits (2310), Expect = 4.3e-256
Identity = 477/536 (88.99%), Postives = 481/536 (89.74%), Query Frame = 0

Query: 1   MAYSSWLLIIVAFCLTVPRSAAVRGFSIREATVPDLQLAFKRNELTSRGLVEFYIGEIRR 60
           MAYSSWLLIIVAFCLTV RSAAVRGFSIREAT+PDLQLAFKRN+LTS GLVEFYIGEIRR
Sbjct: 17  MAYSSWLLIIVAFCLTVQRSAAVRGFSIREATIPDLQLAFKRNQLTSTGLVEFYIGEIRR 76

Query: 61  LNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 120
           LNPVVHGVIEINPDALLQAYK+DREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS
Sbjct: 77  LNPVVHGVIEINPDALLQAYKSDREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGS 136

Query: 121 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR----------- 180
           FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSAR           
Sbjct: 137 FALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARGGQGKNPYVLS 196

Query: 181 -------------VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 240
                        VAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP
Sbjct: 197 ASPCGSSSGPSISVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSP 256

Query: 241 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 300
           RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI
Sbjct: 257 RQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGI 316

Query: 301 VRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEF 360
           VRNPF SFFNDSAITQAFE+HFNILKQGGAILVDNLEI NIDIILNVTASGEAVALLAEF
Sbjct: 317 VRNPFLSFFNDSAITQAFEEHFNILKQGGAILVDNLEIANIDIILNVTASGEAVALLAEF 376

Query: 361 KQSLNEYLKELVASPVRSLADIIAFDNANPD--------------------------QEL 420
           KQSLNEYLKELVASPVRSLADIIAFDNANPD                          QEL
Sbjct: 377 KQSLNEYLKELVASPVRSLADIIAFDNANPDQVTQYSIHCSSHSNRIHSESIRIMVVQEL 436

Query: 421 LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL 480
           LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL
Sbjct: 437 LDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTPGPAIATVL 496

Query: 481 AIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPTLIEVAYAFEQATLIRKPPSFKP 487
           AIGGFPGINVPAGYDGGGVPFGINFGGLKGSEP LIEVAYAFEQATLIRKPPSFKP
Sbjct: 497 AIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPKLIEVAYAFEQATLIRKPPSFKP 552

BLAST of CmoCh19G007360 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 526.2 bits (1354), Expect = 2.9e-149
Identity = 282/488 (57.79%), Postives = 361/488 (73.98%), Query Frame = 0

Query: 3   YSSWLLIIV---AFCLTVPRSAAVR---GFSIREATVPDLQLAFKRNELTSRGLVEFYIG 62
           +SS L++++   A  ++V  ++ +R    FSI+EAT+ D+++AF    LTS+ LVE Y+ 
Sbjct: 9   FSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLE 68

Query: 63  EIRRLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNT 122
            I +LNP++H VIE NPDAL+QA  ADRER+ K    L  LHG+PVLLKDSI TKDKLNT
Sbjct: 69  AISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNT 128

Query: 123 TAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEWADFRSLAAPAGLSARVAANIAA 182
           TAGSFALLGS+V RDAGVVKRLR +GA+ILGKASLSEWA FRS + P G SA        
Sbjct: 129 TAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA-------- 188

Query: 183 VSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLD 242
                          S NSVVGIKP+VGLTSRAGV+P+S RQD+IGPI RTV+DAV +LD
Sbjct: 189 --------------PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLD 248

Query: 243 TIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFED 302
            IVG+D  DEAT+T+S++IP GGYKQFL  +GLKGKRLGIV             +   + 
Sbjct: 249 AIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRLGIVMKH----------SSLLDH 308

Query: 303 HFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVRSLA 362
           H   L++ GAI+++NL I NI++I+  T SGE +ALLAEFK SLN YLKELV SPVRSLA
Sbjct: 309 HIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAEFKMSLNAYLKELVKSPVRSLA 368

Query: 363 DIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERL 422
           D+IA++    +QE +  +GQE+FL AEAT+G+G+ +K AL  + +L+++G EK++EE +L
Sbjct: 369 DVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKL 428

Query: 423 DAVVTPGPAIATVLAIGGFPGINVPAGYDGGGVPFGINFGGLKGSEPTLIEVAYAFEQAT 482
           DA+VT G  +++VLAIGG+PGINVPAGYD GGVP+GI+FGGL+ SEP LIE+A+AFEQAT
Sbjct: 429 DAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISFGGLRFSEPKLIEIAFAFEQAT 464

Query: 483 LIRKPPSF 485
           LIRKPP F
Sbjct: 489 LIRKPPKF 464

BLAST of CmoCh19G007360 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 1.3e-24
Identity = 130/494 (26.32%), Postives = 218/494 (44.13%), Query Frame = 0

Query: 44  ELTSRGLVEFYIGEIRRLNPVVHGVIEINPDALLQAYKADREREAKKPGSLCGLHGIPVL 103
           E T+  + + Y+  IR   P +   + ++ + L  A + D  +   K   L  L G+ + 
Sbjct: 57  ETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID--QRIAKGEELGPLAGVLIG 116

Query: 104 LKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKASLSEW--------A 163
           +KD+I T+  + +TA S  L     P DA  VK+++  G I++GK ++ E+        +
Sbjct: 117 VKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGSTTEAS 176

Query: 164 DFRSLAAP-----------AGLSARVAANIAAVSIGTETDGSILCPASFNSVVGIKPTVG 223
            F+  A P            G +A VAA    VS+G++T GS+  PASF  VVG+KPT G
Sbjct: 177 AFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYG 236

Query: 224 LTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPYGGYKQFL 283
             SR G++  +   D IG  G TV DA ++L  I G+D  D    TSSK        QFL
Sbjct: 237 RVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDS---TSSKQDVPEFQSQFL 296

Query: 284 N-----ANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVDNLEIENIDI 343
           +     +  L G ++GI+R        DS +  A ++  + L+  G IL + + + +  +
Sbjct: 297 SVDHFESKPLNGVKVGIIRETLEDGV-DSGVRSATQEAASHLEALGCILTE-VSLPSFSL 356

Query: 344 IL---NVTASGEAVALLAEFKQSLNEYLKELVASPVRSLADIIAFDNANPDQELLDVFGQ 403
            L    V AS E+ + L+ +      Y  +++A  +  L +    +    + ++  + G 
Sbjct: 357 GLPAYYVIASSESSSNLSRYDGV--RYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGT 416

Query: 404 EIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEEERLDAVVTP-------------- 463
               A     G  D        +  L +  F+  +E+   D +++P              
Sbjct: 417 YALSA-----GYYDAYYKRAQQVRTLIRKDFKAALEQN--DILISPAAPSAAYKIGEKKD 476

Query: 464 -------GPAIATVLAIGGFPGINVPAG-YDGG--GVPFGINFGGLKGSEPTLIEVAYAF 487
                  G  +   + + G P + +P G  +GG  G+P G+   G    E  L++V + F
Sbjct: 477 DPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIF 530

BLAST of CmoCh19G007360 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.3e-21
Identity = 91/336 (27.08%), Postives = 150/336 (44.64%), Query Frame = 0

Query: 32  TVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPD-ALLQAYKADREREAKK 91
           +V +L    K  ++TS+ LV  Y+ +++R N V+  V+    + A  QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 92  PGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 151
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 152 SLSEWA----------------DFRSLAAPAGLSARVAANIAAVSIGTETDGSILCPASF 211
                A                +  S  + AG +A  +A +   +IG+ET GS+  PA+ 
Sbjct: 324 VTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAAR 383

Query: 212 NSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSK 271
             +  ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D ++R  + 
Sbjct: 384 CGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSREIAF 443

Query: 272 YIPYGGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVD--- 331
             P+      L     K   + +V                      +L   G  +V    
Sbjct: 444 EDPFSVDITKLTVGYTKDADMKVV---------------------EVLGSKGVNMVPFEL 503

Query: 332 NLEIENIDIILNVTASGEAVALLAEFKQSLNEYLKE 347
           N  +E++  ILN T   + +A   E++++  E L E
Sbjct: 504 NYTVESVQGILNFTMDVDMLAHFDEWQRTGQEDLYE 514

BLAST of CmoCh19G007360 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 95.9 bits (237), Expect = 9.5e-20
Identity = 91/332 (27.41%), Postives = 147/332 (44.28%), Query Frame = 0

Query: 32  TVPDLQLAFKRNELTSRGLVEFYIGEIRRLNPVVHGVIEINPD-ALLQAYKADREREAKK 91
           +V +L    K  ++TS+ LV  Y+ +++R N V+  V+    + A  QA +AD   +   
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEAD---DLLS 263

Query: 92  PGSLCG-LHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAGVVKRLRRAGAIILGKA 151
            G+  G LHGIP  LKD +       TT GS +     +  +A V KRL+ +GA+++ K 
Sbjct: 264 QGTYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKL 323

Query: 152 SLSEWA------------DFRSLAAPAGLSARVAANIAAVSIGTETDGSILCPASFNSVV 211
                A             +       G SA  AA+ +A   G+ET GS+  PA+   + 
Sbjct: 324 VTGSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSA---GSETAGSMTYPAARCGIT 383

Query: 212 GIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIVLDTIVGFDYNDEATRTSSKYIPY 271
            ++PT G   R GV+ +S   D +GP  RT  D  ++LD I G D +D ++R  +   P+
Sbjct: 384 ALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSREIAFEDPF 443

Query: 272 GGYKQFLNANGLKGKRLGIVRNPFFSFFNDSAITQAFEDHFNILKQGGAILVD---NLEI 331
                 L     K   + +V                      +L   G  +V    N  +
Sbjct: 444 SVDITKLTVGYTKDADMKVV---------------------EVLGSKGVNMVPFELNYTV 503

Query: 332 ENIDIILNVTASGEAVALLAEFKQSLNEYLKE 347
           E++  ILN T   + +A   E++++  E L E
Sbjct: 504 ESVQGILNFTMDVDMLAHFDEWQRTGQEDLYE 507

BLAST of CmoCh19G007360 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 87.4 bits (215), Expect = 3.4e-17
Identity = 108/446 (24.22%), Postives = 190/446 (42.60%), Query Frame = 0

Query: 74  DALLQAYKADREREAKKPGSLCGLHGIPVLLKDSIGTKDKLNTTAGSFALLGSIVPRDAG 133
           + + QA  + R  E   P S+  L GI V +KD I           ++      V +D+ 
Sbjct: 176 EVIKQAEASTRRFEQGNPISV--LDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSA 235

Query: 134 VVKRLRRAGAIILGKASL-----------SEWADFRSLAAP--------AGLSARVAANI 193
           VV +LR  GAI+LGKA++           S +   R+   P        +G +A VAA +
Sbjct: 236 VVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGL 295

Query: 194 AAVSIGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDTIGPIGRTVTDAVIV 253
            + ++GT+  GS+  P++   + G+K T G T   G +      + IGP+  ++ DA +V
Sbjct: 296 CSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDAFLV 355

Query: 254 LDTIVGFDYNDEATRTSSKYIPYGGYKQFLNANGLKGKRLGIVR-NPFFSFFNDSAITQA 313
              I+G   +  A R + K  P   + + L+ NG     +G +R   +  +FND + +  
Sbjct: 356 YAAILG---SSSADRYNLKPSP-PCFPKLLSHNG--SNAIGSLRLGKYTKWFNDVSSSDI 415

Query: 314 FEDHFNILKQGGAILVDNLEIENIDIILNVTASGEAVALLAEFKQSLNEYLKELVASPVR 373
            +   +ILK    +L +N   + ++I++       A  +++    +L+       A    
Sbjct: 416 SDKCEDILK----LLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGKNS 475

Query: 374 SLADIIAFDNANPDQELLDVFGQEIFLAAEATNGIGDVQKAALLNLAKLTKDGFEKVVEE 433
            L+      +      +   F    ++AA+       +++  +     + KD    V   
Sbjct: 476 KLS-----YDTRTSFAIFRSFSASDYIAAQC------LRRRLMEYHLNIFKDVDVIVTPT 535

Query: 434 ERLDAVVTPGPAIAT--------------VLAIG--GFPGINVPAGYDGGGVPFGINFGG 484
             + A V P  A+                VLA    GFP I+VP GYD  G+P G+   G
Sbjct: 536 TGMTAPVIPPDALKNGETNIQVTTDLMRFVLAANLLGFPAISVPVGYDKEGLPIGLQIMG 595

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A1P8B7607.3e-15858.59Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=... [more]
Q9URY42.0e-6735.73Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ... [more]
D4B3C81.0e-5834.01Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ... [more]
B0K3S34.3e-4130.26Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain ... [more]
B0KBN47.3e-4130.26Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanol... [more]
Match NameE-valueIdentityDescription
A0A6J1GLV21.1e-26595.29putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111455539 PE=4 SV=1[more]
A0A6J1HUS82.1e-26193.92putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111468076 PE=4 SV=1[more]
A0A0A0K2I05.7e-23886.76Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009780 PE=... [more]
A0A1S4DXK29.7e-23885.97putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103490301 PE=4... [more]
A0A5A7U6F52.2e-23785.97Putative amidase isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
XP_022953021.12.3e-26595.29putative amidase C869.01 [Cucurbita moschata][more]
KAG7011753.11.2e-26394.90hypothetical protein SDJN02_26659, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6572101.11.1e-26294.71putative amidase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968892.14.4e-26193.92putative amidase C869.01 [Cucurbita maxima][more]
XP_023554278.14.3e-25688.99probable amidase At4g34880 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G34880.12.9e-14957.79Amidase family protein [more]
AT3G25660.11.3e-2426.32Amidase family protein [more]
AT5G07360.11.3e-2127.08Amidase family protein [more]
AT5G07360.29.5e-2027.41Amidase family protein [more]
AT5G64440.13.4e-1724.22fatty acid amide hydrolase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023631Amidase signature domainPFAMPF01425Amidasecoord: 50..156
e-value: 5.9E-22
score: 78.3
coord: 165..424
e-value: 4.2E-34
score: 118.3
IPR036928Amidase signature (AS) superfamilyGENE3D3.90.1300.10Amidase signature (AS) domaincoord: 16..484
e-value: 6.9E-125
score: 419.9
IPR036928Amidase signature (AS) superfamilySUPERFAMILY75304Amidase signature (AS) enzymescoord: 27..483
NoneNo IPR availablePANTHERPTHR42678AMIDASEcoord: 21..169
coord: 170..486
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 170..486
NoneNo IPR availablePANTHERPTHR42678:SF19C869.01-LIKE PROTEIN, PUTATIVE-RELATEDcoord: 21..169

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh19G007360.1CmoCh19G007360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane