CmoCh19G001430 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh19G001430
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	geraniol 8-hydroxylase-like
Location	Cmo_Chr19: 801694 .. 802461 (+)
RNA-Seq Expression	CmoCh19G001430
Synteny	CmoCh19G001430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTTTGGCCTATTTTCTTTGTTTTTGTGTCGCCTTCTTCATTTTGAGGCTTAAATGGAAGACGAGAAGTACATCGAAGCTACCTCCGGGACCAAAGCCTTTGCCGGTCATCGAAAATCTCTTGGATCTCGGCGACAAGCCTCACAGGTCGCTCACCGCCATGGCTAAGATATATGGCTCAATCATGACTTTGAAACTTGGACAAGTTACGGCGGTGGTGGTTTCTTCTTCGGCCATGGCTAAAGAGGTCCTACACACTAACGACCCCTCTTTGTGCGACAGAGCCATTTTAGATTCTCTGACAGCTCACGATCATAACGAAGTCGGACTTCCATGGATTCCTGTTTCTCCTCTCTGGAGGAAATATCGCAAAATATGCAACAATACTCTGTTCGTTGGAAAAATTATGGACGCAAACGAGAATCTTCGCCGGAGGAAAGTGGAAGAGCTCGTGGAGATTGTTCGAAAGAGCGCGTCAAAGGGCGAGGCGGTGGATGTAGGGATATTAGCTTTCACGACTACGCTAAATCTACTCTCGAATGCAATTTTCTCGGTTGTTTTAGCAGATTCGAAATCTGAATTAGCGAGACAGTTCAAGAAATACGTGCACGGATATTTGGAAGAGGCCGGAAACCCAAATTTGAGTGATTATTTTCCCGTGTTGAGGAAGCTCGACATTCAAGGGATGAGGAAGAGGATGAAGATACACATGGGAAGTCTCTTGAAACTTCTAGACTCAATGATAAAACAGAGGATGAATTAG

mRNA sequence

ATGGAGTTTTTGGCCTATTTTCTTTGTTTTTGTGTCGCCTTCTTCATTTTGAGGCTTAAATGGAAGACGAGAAGTACATCGAAGCTACCTCCGGGACCAAAGCCTTTGCCGGTCATCGAAAATCTCTTGGATCTCGGCGACAAGCCTCACAGGTCGCTCACCGCCATGGCTAAGATATATGGCTCAATCATGACTTTGAAACTTGGACAAGTTACGGCGGTGGTGGTTTCTTCTTCGGCCATGGCTAAAGAGGTCCTACACACTAACGACCCCTCTTTGTGCGACAGAGCCATTTTAGATTCTCTGACAGCTCACGATCATAACGAAGTCGGACTTCCATGGATTCCTGTTTCTCCTCTCTGGAGGAAATATCGCAAAATATGCAACAATACTCTGTTCGTTGGAAAAATTATGGACGCAAACGAGAATCTTCGCCGGAGGAAAGTGGAAGAGCTCGTGGAGATTGTTCGAAAGAGCGCGTCAAAGGGCGAGGCGGTGGATGTAGGGATATTAGCTTTCACGACTACGCTAAATCTACTCTCGAATGCAATTTTCTCGGTTGTTTTAGCAGATTCGAAATCTGAATTAGCGAGACAGTTCAAGAAATACGTGCACGGATATTTGGAAGAGGCCGGAAACCCAAATTTGAGTGATTATTTTCCCGTGTTGAGGAAGCTCGACATTCAAGGGATGAGGAAGAGGATGAAGATACACATGGGAAGTCTCTTGAAACTTCTAGACTCAATGATAAAACAGAGGATGAATTAG

Coding sequence (CDS)

ATGGAGTTTTTGGCCTATTTTCTTTGTTTTTGTGTCGCCTTCTTCATTTTGAGGCTTAAATGGAAGACGAGAAGTACATCGAAGCTACCTCCGGGACCAAAGCCTTTGCCGGTCATCGAAAATCTCTTGGATCTCGGCGACAAGCCTCACAGGTCGCTCACCGCCATGGCTAAGATATATGGCTCAATCATGACTTTGAAACTTGGACAAGTTACGGCGGTGGTGGTTTCTTCTTCGGCCATGGCTAAAGAGGTCCTACACACTAACGACCCCTCTTTGTGCGACAGAGCCATTTTAGATTCTCTGACAGCTCACGATCATAACGAAGTCGGACTTCCATGGATTCCTGTTTCTCCTCTCTGGAGGAAATATCGCAAAATATGCAACAATACTCTGTTCGTTGGAAAAATTATGGACGCAAACGAGAATCTTCGCCGGAGGAAAGTGGAAGAGCTCGTGGAGATTGTTCGAAAGAGCGCGTCAAAGGGCGAGGCGGTGGATGTAGGGATATTAGCTTTCACGACTACGCTAAATCTACTCTCGAATGCAATTTTCTCGGTTGTTTTAGCAGATTCGAAATCTGAATTAGCGAGACAGTTCAAGAAATACGTGCACGGATATTTGGAAGAGGCCGGAAACCCAAATTTGAGTGATTATTTTCCCGTGTTGAGGAAGCTCGACATTCAAGGGATGAGGAAGAGGATGAAGATACACATGGGAAGTCTCTTGAAACTTCTAGACTCAATGATAAAACAGAGGATGAATTAG

Protein sequence

MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIYGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPLWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLLSNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMGSLLKLLDSMIKQRMN

Homology

[Close]

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot
Match: D1MI46 (Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 4.8e-60
Identity = 119/236 (50.42%), Postives = 163/236 (69.07%), Query Frame = 0


Query: 19  LKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIYGSIMTLKLGQVTAVVVSS 78
           L + +R +  LPPGP PLP+I NL  LGD+PH+SL  +AK +G IM L+LGQVT +VV+S
Sbjct: 22  LNFFSRKSKNLPPGPSPLPLIGNLHLLGDQPHKSLAKLAKKHGPIMGLQLGQVTTIVVTS 81

Query: 79  SAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPLWRKYRKICNNTLFVGKIM 138
           S MAKEVL   D +   R+I +++ AHD  +  + W+PV+  WR  RK  N+ +F G  +
Sbjct: 82  SGMAKEVLQKQDLAFSSRSIPNAIHAHDQYKYSVIWLPVASRWRGLRKALNSNMFSGNRL 141

Query: 139 DANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLLSNAIFSVVLADSKSELAR 198
           DAN++LR RKV+EL+   RKS+  G+A+DVG  AF T+LNLLSN +FS  L D  S+ A+
Sbjct: 142 DANQHLRSRKVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAK 201

Query: 199 QFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMGSLLKLLDSMIKQRM 255
           +FK  V   + EAG PNL DYFP+L K+D QG+RKRM IH G +L+L   +I +R+
Sbjct: 202 EFKDLVWNVMVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERL 257

[Close]

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.1e-59
Identity = 120/255 (47.06%), Postives = 169/255 (66.27%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILR-LKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKI 60
           M++L   L    A  +     + +R T  LPPGP PLP I +L  LGD+PH+SL  ++K 
Sbjct: 1   MDYLTIILTLLFALTLYEAFSYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKK 60

Query: 61  YGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSP 120
           +G IM+LKLGQ+T +V+SSS MAKEVL   D +   R++ ++L AH+  +  + W+PV+ 
Sbjct: 61  HGPIMSLKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVAS 120

Query: 121 LWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNL 180
            WR  RK+ N+ +F G  +DAN++LR RKV+EL+   RK++  GEAVDVG  AF T+LNL
Sbjct: 121 RWRSLRKVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNL 180

Query: 181 LSNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHM 240
           LSN IFS  L D  S+ A++FK  V   + EAG PNL D+FP+L K+D QG+R RM IH 
Sbjct: 181 LSNLIFSKDLTDPYSDSAKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHF 240

Query: 241 GSLLKLLDSMIKQRM 255
           G +LKL   ++ +R+
Sbjct: 241 GEVLKLFGGLVNERL 255

[Close]

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot
Match: P0DKI2 (Inactive cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=3 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 2.7e-55
Identity = 107/243 (44.03%), Postives = 165/243 (67.90%), Query Frame = 0


Query: 12  VAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIYGSIMTLKLGQV 71
           ++F  ++L +  ++T  LPPGPKPLP+I N+L++G KPHRS   +AKI+G +++L+LG V
Sbjct: 15  ISFHFIKLLFSQQTTKLLPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGPLISLRLGSV 74

Query: 72  TAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPLWRKYRKICNNT 131
           T +VVSS+ +AKE+    D  L +R I +S+TA DH+++ + W+PVSP WR +RKI    
Sbjct: 75  TTIVVSSADVAKEMFLKKDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWRNFRKITAVH 134

Query: 132 LFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLLSNAIFSVVLAD 191
           L   + +DA +  R  KV++L E V++ A KG+AVD+G  AFTT+LNLLS   FSV LA 
Sbjct: 135 LLSPQRLDACQTFRHAKVQQLYEYVQECAQKGQAVDIGKAAFTTSLNLLSKLFFSVELAH 194

Query: 192 SKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMGSLLKLLDSMIK 251
            KS  +++FK+ +   +E+ G PN +DYFP+L  +D  G+R+R+      L+ +  S+I 
Sbjct: 195 HKSHTSQEFKELIWNIMEDIGKPNYADYFPILGCVDPSGIRRRLACSFDKLIAVFQSIIC 254

Query: 252 QRM 255
           +R+
Sbjct: 255 ERL 257

[Close]

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot
Match: I3PFJ5 (Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 7.9e-55
Identity = 106/243 (43.62%), Postives = 164/243 (67.49%), Query Frame = 0


Query: 12  VAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIYGSIMTLKLGQV 71
           ++F  ++L +  ++T  LPPGPKPLP+I N+L++G KPHRS   +AKI+G +++L+LG V
Sbjct: 15  ISFHFIKLLFSQQTTKLLPPGPKPLPIIGNILEVGKKPHRSFANLAKIHGPLISLRLGSV 74

Query: 72  TAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPLWRKYRKICNNT 131
           T +VVSS+ +AKE+    D  L +R I +S+TA DH+++ + W+PVSP WR +RKI    
Sbjct: 75  TTIVVSSADVAKEMFLKKDHPLSNRTIPNSVTAGDHHKLTMSWLPVSPKWRNFRKITAVH 134

Query: 132 LFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLLSNAIFSVVLAD 191
           L   + +DA +  R  KV++L E V++ A KG+AVD+G  AFTT+LNLLS   FSV LA 
Sbjct: 135 LLSPQRLDACQTFRHAKVQQLYEYVQECAQKGQAVDIGKAAFTTSLNLLSKLFFSVELAH 194

Query: 192 SKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMGSLLKLLDSMIK 251
            KS  +++FK+ +   +E+ G PN +DYFP+L  +D  G+R+R+      L+ +   +I 
Sbjct: 195 HKSHTSQEFKELIWNIMEDIGKPNYADYFPILGCVDPSGIRRRLACSFDKLIAVFQGIIC 254

Query: 252 QRM 255
           +R+
Sbjct: 255 ERL 257

[Close]

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot
Match: W0FKI0 (Drimenol monooxygenase OS=Persicaria hydropiper OX=46901 GN=CYP76AJ1 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-53
Identity = 110/256 (42.97%), Postives = 168/256 (65.62%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTR-STSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKI 60
           M++L   +C  V+  +L   + +R S  KLPPGP  LP++ NL DLG  PHRSL  +AK+
Sbjct: 1   MDYLILMICIVVSGLLL---YSSRTSKMKLPPGPPGLPIVGNLFDLGSLPHRSLAKLAKL 60

Query: 61  YGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSP 120
           +G +M L++G++  VV+SS + AKEVL T+D   C+R + DSLTA  H+   +  +P + 
Sbjct: 61  HGPVMCLRMGRLRVVVISSDSAAKEVLQTSDTLFCNRFVYDSLTASQHHTFSMALLPPTA 120

Query: 121 LWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNL 180
           LW+  RKI  + LF    M+A+++LRR+++E+L+  V   +  G A+++   AF T++NL
Sbjct: 121 LWKSLRKISASQLFTNARMNASQHLRRKQLEDLLSYVESCSRSGTAINIAQAAFNTSVNL 180

Query: 181 LSNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHM 240
           LS   FSV L D  S  + +FK+ V   + E+G PNL+DYFPVLR++D QG R+RMKI  
Sbjct: 181 LSKTFFSVDLIDPSSSNSVEFKEMVWQIMLESGTPNLADYFPVLRRIDPQGNRRRMKIQF 240

Query: 241 GSLLKLLDSMIKQRMN 256
             +L L ++MI+QR++
Sbjct: 241 EKILDLFNTMIRQRLD 253

[Close]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL
Match: A0A6J1HL81 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463981 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 2.7e-138
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY 60
           MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY
Sbjct: 1   MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY 60

Query: 61  GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL 120
           GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL
Sbjct: 61  GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL 120

Query: 121 WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL 180
           WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL
Sbjct: 121 WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL 180

Query: 181 SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG 240
           SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG
Sbjct: 181 SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG 240

Query: 241 SLLKLLDSMIKQRMN 256
           SLLKLLDSMIKQRMN
Sbjct: 241 SLLKLLDSMIKQRMN 255

[Close]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL
Match: A0A6J1HGL8 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463910 PE=3 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.6e-114
Identity = 217/254 (85.43%), Postives = 229/254 (90.16%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY 60
           MEFLA FLCFCVAFFILRLK K RSTSKLPPGPKPLPVI NLLDLGDKPH+SL AMAK+Y
Sbjct: 1   MEFLACFLCFCVAFFILRLKSKARSTSKLPPGPKPLPVIGNLLDLGDKPHKSLAAMAKVY 60

Query: 61  GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL 120
           G I++LKLG+VTAVVVSSSAMAKEVL TND SLCDRAI DSLTA+DHNEVG PWI VSPL
Sbjct: 61  GPILSLKLGRVTAVVVSSSAMAKEVLQTNDHSLCDRAIPDSLTAYDHNEVGFPWISVSPL 120

Query: 121 WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL 180
           WRKYRKICNNTLF GKI+DANENLRR+KVEELVEIVRKSASKGEAVDVG L FT TLNLL
Sbjct: 121 WRKYRKICNNTLFAGKILDANENLRRKKVEELVEIVRKSASKGEAVDVGRLMFTITLNLL 180

Query: 181 SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG 240
           SN IFSV LAD KSELARQFKK V   +EEAG PNLSDYFPVLRKLDIQGMRKRMKIH+G
Sbjct: 181 SNTIFSVDLADPKSELARQFKKCVQETMEEAGKPNLSDYFPVLRKLDIQGMRKRMKIHLG 240

Query: 241 SLLKLLDSMIKQRM 255
             LK+LDSM+KQRM
Sbjct: 241 GFLKILDSMVKQRM 254

[Close]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL
Match: A0A6J1HIG9 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463908 PE=3 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.3e-113
Identity = 215/254 (84.65%), Postives = 227/254 (89.37%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY 60
           MEFLA FLCFCVAFFILRLK K RSTSKLPPGPKPLPVI NLLDLGDKPH+SL AMAK+Y
Sbjct: 1   MEFLACFLCFCVAFFILRLKSKARSTSKLPPGPKPLPVIGNLLDLGDKPHKSLAAMAKVY 60

Query: 61  GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL 120
           G IM+LKLG+VTAVVVSSSAMAKEVL TND SLCDR + DSLTAHDHNEVG  WI VSPL
Sbjct: 61  GPIMSLKLGRVTAVVVSSSAMAKEVLQTNDHSLCDRTVPDSLTAHDHNEVGFAWISVSPL 120

Query: 121 WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL 180
           WRKYRKIC NTLF GKI+DANENLRR+KVEELVEIVRKSASKGEAVDVG L FT TLN L
Sbjct: 121 WRKYRKICKNTLFAGKILDANENLRRKKVEELVEIVRKSASKGEAVDVGRLVFTITLNQL 180

Query: 181 SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG 240
           SN IFSV LAD KSELAR+FKKYV G +EE G PNLSDYFPVLRK DIQGMRKRM+IHMG
Sbjct: 181 SNTIFSVDLADPKSELARRFKKYVQGIMEEGGKPNLSDYFPVLRKFDIQGMRKRMEIHMG 240

Query: 241 SLLKLLDSMIKQRM 255
           S+L+LLDSMIKQRM
Sbjct: 241 SVLELLDSMIKQRM 254

[Close]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL
Match: A0A6J1HKS6 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463909 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.4e-110
Identity = 209/253 (82.61%), Postives = 225/253 (88.93%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIY 60
           MEFLAYFLCFC AFFILRLKWKTRST KLPPGPKPLPVI NLLDLGDKPH+SL AMAK+Y
Sbjct: 1   MEFLAYFLCFCAAFFILRLKWKTRSTQKLPPGPKPLPVIGNLLDLGDKPHKSLAAMAKVY 60

Query: 61  GSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSPL 120
           G IM+LKLG+VTAVVVSSSAMAKEVL TN+ SLCDRAI DSLTA+DHNEVG PWI VSPL
Sbjct: 61  GPIMSLKLGRVTAVVVSSSAMAKEVLQTNEHSLCDRAIPDSLTAYDHNEVGFPWISVSPL 120

Query: 121 WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLL 180
           WRKYRKICN+TLF  KI+DANENLRR+KVEELVEIVRKSA KGEAVD+G L FTTTLNLL
Sbjct: 121 WRKYRKICNSTLFARKILDANENLRRKKVEELVEIVRKSALKGEAVDIGRLLFTTTLNLL 180

Query: 181 SNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMG 240
           SN IFSV LA  KSELARQFKK V G +E AG  NLSDYFP+LRKLDIQGMRKRM+IHMG
Sbjct: 181 SNTIFSVDLAYPKSELARQFKKCVQGMMELAGKSNLSDYFPMLRKLDIQGMRKRMEIHMG 240

Query: 241 SLLKLLDSMIKQR 254
           S  K+LDSM++ R
Sbjct: 241 SFFKILDSMVELR 253

[Close]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL
Match: A0A6J1K4E7 (geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491590 PE=3 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 5.3e-102
Identity = 197/255 (77.25%), Postives = 219/255 (85.88%), Query Frame = 0


Query: 1   MEFLAYFLCFCVA-FFILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKI 60
           MEF    LC CVA FFIL+LK KT +TSKLPPGPKPLPVI NLLDLG+KPH+SL AMAK+
Sbjct: 1   MEFWTCILCLCVAFFFILKLKSKTGNTSKLPPGPKPLPVIGNLLDLGEKPHKSLAAMAKV 60

Query: 61  YGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSP 120
           +G IM++KLG+VTAVVVSSS MAKEVL TND  LC+RAI D+L AH H+EVG PWI VSP
Sbjct: 61  HGPIMSMKLGRVTAVVVSSSTMAKEVLQTNDQFLCNRAIPDALKAHAHDEVGFPWISVSP 120

Query: 121 LWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNL 180
           LWR YRKICNNTLF GKI+DANENLRRRKVEELVEIVRKSASKGEAVD+G L F TTLNL
Sbjct: 121 LWRNYRKICNNTLFAGKILDANENLRRRKVEELVEIVRKSASKGEAVDLGRLLFMTTLNL 180

Query: 181 LSNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHM 240
           +SN +FSV LAD  SELAR+FK YV G LEEAG PNLSDYFPVLRKLDIQG+R+RMKI M
Sbjct: 181 ISNTVFSVDLADPNSELARKFKNYVWGILEEAGKPNLSDYFPVLRKLDIQGVRRRMKIQM 240

Query: 241 GSLLKLLDSMIKQRM 255
           G++L L+DSMI QRM
Sbjct: 241 GNVLDLIDSMINQRM 255

[Close]

BLAST of CmoCh19G001430 vs. TAIR 10
Match: AT2G45580.1 (cytochrome P450, family 76, subfamily C, polypeptide 3 )

HSP 1 Score: 183.7 bits (465), Expect = 1.8e-46
Identity = 109/257 (42.41%), Postives = 152/257 (59.14%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKW-KTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKI 60
           M    YFL     FF    K  ++ ST  LPPGP  LP++ N+  LG  PHRSL A +K 
Sbjct: 9   MSLPLYFLLTLFFFFFATAKTRRSSSTGTLPPGPPILPLVGNIFQLGFNPHRSLAAFSKT 68

Query: 61  YGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSP 120
           YG IM+LKLG++TAVV+SS   AKE L T+D  +  R   D+L A DH++  + WIP S 
Sbjct: 69  YGPIMSLKLGRLTAVVISSPEAAKEALRTHDHVMSARTFNDALRAFDHHKHSIVWIPPSA 128

Query: 121 LWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNL 180
            WR  +K     L   + +DA ++LR RKVEELV +V +   +GEA+D+   +F T+ N+
Sbjct: 129 RWRFLKKTITKYLLSPQNLDAIQSLRMRKVEELVSLVNEFRERGEAIDLARASFVTSFNI 188

Query: 181 LSNAIFSVVLA--DSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKI 240
           +SNA+FSV LA  DS S  + +F   V    + AG PN+ DYF  +R LD+QG RK+  +
Sbjct: 189 ISNALFSVDLATYDSNSS-SYEFHNTVVHLTDIAGIPNVGDYFQYMRFLDLQGTRKKAVL 248

Query: 241 HMGSLLKLLDSMIKQRM 255
            +  L ++    I  R+
Sbjct: 249 CIEKLFRVFQEFIDARL 264

[Close]

BLAST of CmoCh19G001430 vs. TAIR 10
Match: AT3G61040.1 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 176.0 bits (445), Expect = 3.8e-44
Identity = 102/255 (40.00%), Postives = 149/255 (58.43%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRST----SKLPPGPKPLPVIENLLDLGDKPHRSLTAM 60
           M+ +A  L     FF+    + T  +    +K PPGP  L ++ N+L   +KPHRSL  +
Sbjct: 1   MDIVAIVLSLLFIFFLFFFFYTTGKSCPGGAKNPPGPSKLSLLRNILQTVEKPHRSLADL 60

Query: 61  AKIYGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIP 120
           ++IYGS+M+ KLG +T VV+SS   AKEVL T+D  L  R   D + A  H+E+ L WIP
Sbjct: 61  SRIYGSVMSFKLGCLTTVVISSPETAKEVLKTHDHVLSYRVSSDPVRAAGHHELSLLWIP 120

Query: 121 VSPLWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTT 180
               WR  RKI  N LF  + ++A   +R RKV+EL+  V K   + EAV++   +F T+
Sbjct: 121 PLARWRFLRKITRNQLFSTQRLEATSAIRTRKVQELMNFVNKCCERREAVNISRASFITS 180

Query: 181 LNLLSNAIFSVVLAD-SKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRM 240
           LN++SNA+FS  LA+   S+    F+  V   +E +G PNL+D+FP L  LD+QG RK  
Sbjct: 181 LNIISNALFSTNLANFDDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEA 240

Query: 241 KIHMGSLLKLLDSMI 251
           ++ M  L ++    I
Sbjct: 241 RLLMHKLFRVFQGFI 255

[Close]

BLAST of CmoCh19G001430 vs. TAIR 10
Match: AT3G61040.2 (cytochrome P450, family 76, subfamily C, polypeptide 7 )

HSP 1 Score: 176.0 bits (445), Expect = 3.8e-44
Identity = 102/255 (40.00%), Postives = 149/255 (58.43%), Query Frame = 0


Query: 1   MEFLAYFLCFCVAFFILRLKWKTRST----SKLPPGPKPLPVIENLLDLGDKPHRSLTAM 60
           M+ +A  L     FF+    + T  +    +K PPGP  L ++ N+L   +KPHRSL  +
Sbjct: 1   MDIVAIVLSLLFIFFLFFFFYTTGKSCPGGAKNPPGPSKLSLLRNILQTVEKPHRSLADL 60

Query: 61  AKIYGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIP 120
           ++IYGS+M+ KLG +T VV+SS   AKEVL T+D  L  R   D + A  H+E+ L WIP
Sbjct: 61  SRIYGSVMSFKLGCLTTVVISSPETAKEVLKTHDHVLSYRVSSDPVRAAGHHELSLLWIP 120

Query: 121 VSPLWRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTT 180
               WR  RKI  N LF  + ++A   +R RKV+EL+  V K   + EAV++   +F T+
Sbjct: 121 PLARWRFLRKITRNQLFSTQRLEATSAIRTRKVQELMNFVNKCCERREAVNISRASFITS 180

Query: 181 LNLLSNAIFSVVLAD-SKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRM 240
           LN++SNA+FS  LA+   S+    F+  V   +E +G PNL+D+FP L  LD+QG RK  
Sbjct: 181 LNIISNALFSTNLANFDDSKTFHDFQNVVIRMMEISGKPNLADFFPFLGFLDLQGARKEA 240

Query: 241 KIHMGSLLKLLDSMI 251
           ++ M  L ++    I
Sbjct: 241 RLLMHKLFRVFQGFI 255

[Close]

BLAST of CmoCh19G001430 vs. TAIR 10
Match: AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-42
Identity = 96/250 (38.40%), Postives = 150/250 (60.00%), Query Frame = 0


Query: 7   FLCFCVAFF-ILRLKWKTRSTSKLPPGPKPLPVIENLLDLGDKPHRSLTAMAKIYGSIMT 66
           F+  C+ FF   R +      SK PPGP  LP+I N+  +G  PH S T ++K YG +M+
Sbjct: 15  FILSCLLFFTTARSRRSPCQLSKSPPGPPRLPIIGNIHLVGKNPHHSFTDLSKTYGPVMS 74

Query: 67  LKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWI-PVSPLWRKY 126
           LKLG + +VV++S    +EVL T+D  L  R I ++  +++H+E  + WI P S  +R  
Sbjct: 75  LKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSNNHHEFSVGWIHPSSSRFRML 134

Query: 127 RKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLNLLSNAI 186
           RK+    LF  + + A + LR +KV+ELV  + +S  + EAVD+  ++F T LN++SN +
Sbjct: 135 RKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISHVSFVTALNIISNIL 194

Query: 187 FSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMKIHMGSLLK 246
           FSV L    S+ +  F++ V GY E  GNP+L+++FP +R LD+QG  K+M+   G LL+
Sbjct: 195 FSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQGNSKKMRESSGRLLQ 254

Query: 247 LLDSMIKQRM 255
           +       R+
Sbjct: 255 VFREFYDARI 264

[Close]

BLAST of CmoCh19G001430 vs. TAIR 10
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )

HSP 1 Score: 169.5 bits (428), Expect = 3.6e-42
Identity = 94/238 (39.50%), Postives = 142/238 (59.66%), Query Frame = 0


Query: 4   LAYFLCFCVAFFILRLKWKTRSTS----KLPPGPKPLPVIENLLDLGDKPHRSLTAMAKI 63
           L    CF  + F++    ++R +S     LPPGP  LP+I N+  +G  PH S   +AKI
Sbjct: 9   LFLLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKI 68

Query: 64  YGSIMTLKLGQVTAVVVSSSAMAKEVLHTNDPSLCDRAILDSLTAHDHNEVGLPWIPVSP 123
           YG IM+LK G + +VV++S   A+EVL T+D  L  R   DS+    H EV + W+P S 
Sbjct: 69  YGPIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGRKSNDSIRCFGHEEVSVIWLPPSS 128

Query: 124 L-WRKYRKICNNTLFVGKIMDANENLRRRKVEELVEIVRKSASKGEAVDVGILAFTTTLN 183
             WR  RK+    +F  +  +A + LR +KV+ELV  + +S+ + EAVD+   ++TT LN
Sbjct: 129 ARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTVLN 188

Query: 184 LLSNAIFSVVLADSKSELARQFKKYVHGYLEEAGNPNLSDYFPVLRKLDIQGMRKRMK 237
           ++SN +FSV L    S+ + +F+  V G +E AG P+ ++YFP +  LD+QG RK M+
Sbjct: 189 IISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMR 246

The following BLAST results are available for this feature:

BLAST of CmoCh19G001430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D1MI46	4.8e-60	50.42	Geraniol 8-hydroxylase OS=Swertia mussotii OX=137888 GN=CYP76B10 PE=1 SV=1	[more]
Q8VWZ7	1.1e-59	47.06	Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1	[more]
P0DKI2	2.7e-55	44.03	Inactive cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=3 SV=1	[more]
I3PFJ5	7.9e-55	43.62	Cytochrome P450 76AD1 OS=Beta vulgaris OX=161934 GN=CYP76AD1 PE=2 SV=1	[more]
W0FKI0	1.1e-53	42.97	Drimenol monooxygenase OS=Persicaria hydropiper OX=46901 GN=CYP76AJ1 PE=1 SV=1	[more]

BLAST of CmoCh19G001430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HL81	2.7e-138	100.00	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463981 PE=4 S...	[more]
A0A6J1HGL8	4.6e-114	85.43	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463910 PE=3 S...	[more]
A0A6J1HIG9	2.3e-113	84.65	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463908 PE=3 S...	[more]
A0A6J1HKS6	1.4e-110	82.61	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111463909 PE=3 S...	[more]
A0A6J1K4E7	5.3e-102	77.25	geraniol 8-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111491590 PE=3 SV=...	[more]

BLAST of CmoCh19G001430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45580.1	1.8e-46	42.41	cytochrome P450, family 76, subfamily C, polypeptide 3	[more]
AT3G61040.1	3.8e-44	40.00	cytochrome P450, family 76, subfamily C, polypeptide 7	[more]
AT3G61040.2	3.8e-44	40.00	cytochrome P450, family 76, subfamily C, polypeptide 7	[more]
AT1G33720.1	3.6e-42	38.40	cytochrome P450, family 76, subfamily C, polypeptide 6	[more]
AT2G45550.1	3.6e-42	39.50	cytochrome P450, family 76, subfamily C, polypeptide 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 30..253 e-value: 1.8E-26 score: 92.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 15..255 e-value: 3.9E-38 score: 133.2
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 24..254
None	No IPR available	PANTHER	PTHR24299	CYTOCHROME P450 FAMILY 1	coord: 5..254
None	No IPR available	PANTHER	PTHR24299:SF38	CYTOCHROME P450 SUPERFAMILY PROTEIN	coord: 5..254

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G001430.1	CmoCh19G001430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen