CmoCh19G000660 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh19G000660
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Pumpkin fruit trypsin inhibitor
Location	Cmo_Chr19: 377622 .. 377971 (-)
RNA-Seq Expression	CmoCh19G000660
Synteny	CmoCh19G000660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGCAACAACAAAAACAATGTCAGGTAGTAATTTTTAATGCAATGATGTATTGATATTAGTTCAATATGTATTGATATCATTTTATGTATCTAATAAGTATAGTTTACATGGTGAAGTTATGTTGAATGAAGAAAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGAAAGCAGTGAAGATAATTCAACAAGACAACCCTTCTCTGTATGTAATTCTAATGCCACGAGGTGAAGAGGCCGCATCGGCCAATTTTATGTTCAAACGAGTCCGAGTCTTTGTTGACTATTGTGGAAGGGTGAATTCGATCCCTCGAATTGGCTAAGCACTTTTTGACTATATGTAAC

mRNA sequence

CGCAACAACAAAAACAATGTCAGTTATGTTGAATGAAGAAAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGAAAGCAGTGAAGATAATTCAACAAGACAACCCTTCTCTGTATGTAATTCTAATGCCACGAGGTGAAGAGGCCGCATCGGCCAATTTTATGTTCAAACGAGTCCGAGTCTTTGTTGACTATTGTGGAAGGGTGAATTCGATCCCTCGAATTGGCTAAGCACTTTTTGACTATATGTAAC

Coding sequence (CDS)

ATGTCAGTTATGTTGAATGAAGAAAAGTGGCGTTGGCCAGAGTTGGTGGGGATGGATGGGGAGAAAGCAGTGAAGATAATTCAACAAGACAACCCTTCTCTGTATGTAATTCTAATGCCACGAGGTGAAGAGGCCGCATCGGCCAATTTTATGTTCAAACGAGTCCGAGTCTTTGTTGACTATTGTGGAAGGGTGAATTCGATCCCTCGAATTGGCTAA

Protein sequence

MSVMLNEEKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNSIPRIG

Homology

[Close]

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot
Match: P01053 (Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 7.8e-08
Identity = 26/64 (40.62%), Postives = 37/64 (57.81%), Query Frame = 0


Query: 9  KWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNSI 68
          K  WPELVG   E+A K+I QD P   +I++P G    +  +   RVR+FVD    +  +
Sbjct: 22 KTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVG-TIVTMEYRIDRVRLFVDKLDNIAQV 81

Query: 69 PRIG 73
          PR+G
Sbjct: 82 PRVG 84

[Close]

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot
Match: P82977 (Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 2.3e-07
Identity = 24/67 (35.82%), Postives = 39/67 (58.21%), Query Frame = 0


Query: 6  NEEKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRV 65
          + +K  WPELVG   E+A K+I QD     ++++P G    +  +   RVR+FVD   ++
Sbjct: 19 HNQKTEWPELVGKSVEEAKKVILQDKSEAQIVVLPVG-TIVTMEYRIDRVRLFVDSLDKI 78

Query: 66 NSIPRIG 73
            +PR+G
Sbjct: 79 AQVPRVG 84

[Close]

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot
Match: P08626 (Subtilisin-chymotrypsin inhibitor-2B (Fragment) OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.9e-07
Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = 0


Query: 8  EKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNS 67
          +K  WPELV    E+A K+I QD P   +I++P G    +  +   RVR+FVD    +  
Sbjct: 9  QKTEWPELVEKSVEEAKKVILQDKPEAQIIVLPVG-TIVTMEYRIDRVRLFVDRLDNIAQ 68

Query: 68 IPRIG 73
          +PR+G
Sbjct: 69 VPRVG 72

[Close]

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 1.6e-05
Identity = 26/61 (42.62%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 12 WPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNSIPRI 71
          WPELVG +G+ A  IIQ +N ++  I++  G    + +  F RVRVFVD    V  +P I
Sbjct: 11 WPELVGTNGDIAAGIIQTENANVKAIVVKEG-LPITQDLNFNRVRVFVDENRVVTQVPAI 70

Query: 72 G 73
          G
Sbjct: 71 G 70

[Close]

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot
Match: P16231 (Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 8.1e-05
Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 0


Query: 12  WPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNSIPRI 71
           WPEL+G+    A  II+++NPS+  I +       + +F   RVR+FV+  G V  IPR+
Sbjct: 51  WPELIGVPALYAKGIIEKENPSITNIPILLNGSPVTKDFRCDRVRLFVNILGDVVQIPRV 110

[Close]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL
Match: Q43421 (Pumpkin fruit trypsin inhibitor OS=Cucurbita maxima OX=3661 GN=pfiAF4 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.1e-16
Identity = 45/65 (69.23%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 8  EKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNS 67
          EK  WPELVG DGE+AVKIIQQ+NPSL VILMPRG+  A+ ++   RVRVF D  G+VNS
Sbjct: 3  EKLSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATKDYRPNRVRVFNDDSGKVNS 62

Query: 68 IPRIG 73
          IPRIG
Sbjct: 63 IPRIG 67

[Close]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL
Match: Q6DKU8 (Putative chymotrypsin protease inhibitor OS=Cucurbita maxima OX=3661 PE=3 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 8.1e-16
Identity = 45/65 (69.23%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 8  EKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNS 67
          EK  WPELVG DGE+AVKIIQQ+NPSL VILMPRG+  A+ ++   RVRVF D  G+VNS
Sbjct: 3  EKSSWPELVGKDGEEAVKIIQQENPSLDVILMPRGQNWATLDYRPNRVRVFNDESGKVNS 62

Query: 68 IPRIG 73
          IPRIG
Sbjct: 63 IPRIG 67

[Close]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL
Match: Q43420 (Pumpkin Fruit Chymotrypsin Inhibitor OS=Cucurbita maxima OX=3661 GN=pfiBM7 PE=2 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 4.0e-15
Identity = 45/65 (69.23%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 8  EKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNS 67
          EK  WPELVG DGE+AVKIIQQ+NPSL VILMPRG+  A+ +    RVRVF D  G+VNS
Sbjct: 3  EKSSWPELVGEDGEEAVKIIQQENPSLDVILMPRGQNWATLDCRPNRVRVFNDESGKVNS 62

Query: 68 IPRIG 73
          IPRIG
Sbjct: 63 IPRIG 67

[Close]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL
Match: A0A2G9GNE2 (Uncharacterized protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_20721 PE=3 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 1.4e-07
Identity = 32/63 (50.79%), Postives = 39/63 (61.90%), Query Frame = 0


Query: 7  EEKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVN 66
          E K  WPELVG  GE AVKII++D P  Y I++P  ++    NF+  RV VFVD  G V 
Sbjct: 29 EGKQSWPELVGYCGETAVKIIEKDGPGTYAIIVPPDQQHLPGNFVCGRVFVFVDEKGIVE 88

Query: 67 SIP 70
           IP
Sbjct: 89 RIP 91

[Close]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL
Match: A0A1R3I780 (Proteinase inhibitor I13, potato inhibitor I OS=Corchorus capsularis OX=210143 GN=CCACVL1_14397 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 3.1e-07
Identity = 31/72 (43.06%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 1  MSVMLNEEKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVD 60
          MS   +E K  WPELVG++GE A  +I ++NP +  +++  G    + +F   RVRV+VD
Sbjct: 1  MSGCSDEGKSSWPELVGVNGEAAAAVIMEENPKVGAVIVKEG-MMVTMDFRCDRVRVWVD 60

Query: 61 YCGRVNSIPRIG 73
           CG V  IP IG
Sbjct: 61 KCGIVKEIPHIG 71

[Close]

BLAST of CmoCh19G000660 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 54.3 bits (129), Expect = 4.7e-08
Identity = 26/65 (40.00%), Postives = 41/65 (63.08%), Query Frame = 0


Query: 8  EKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNS 67
          +K+RWPELVG +G+ A   I+++NP++  I++  GE+    NF   RV V++   G+V  
Sbjct: 22 KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIE-NFCCNRVFVYLGSNGQVAD 81

Query: 68 IPRIG 73
           P IG
Sbjct: 82 APMIG 85

[Close]

BLAST of CmoCh19G000660 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 51.2 bits (121), Expect = 4.0e-07
Identity = 26/71 (36.62%), Postives = 41/71 (57.75%), Query Frame = 0


Query: 2  SVMLNEEKWRWPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDY 61
          S+  +  K  WPEL+G  GE A ++I+++NP +  +++  G       F+  RV V+V+ 
Sbjct: 30 SICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEI-FICSRVYVWVND 89

Query: 62 CGRVNSIPRIG 73
          CG V  IP IG
Sbjct: 90 CGIVVQIPIIG 99

[Close]

BLAST of CmoCh19G000660 vs. TAIR 10
Match: AT3G50020.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.3e-05
Identity = 25/64 (39.06%), Postives = 38/64 (59.38%), Query Frame = 0


Query: 12 WPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFK-RVRVFVDYC--GRVNSI 71
          WPEL G+ G +A +II+ DNP + V+++P      + N   +  VRV V+ C  G V++ 
Sbjct: 33 WPELNGVKGLEAKRIIEHDNPKVVVVIIPDDVAVLAVNCCNRVIVRVPVNNCPNGPVSNF 92

Query: 72 PRIG 73
          P IG
Sbjct: 93 PHIG 96

[Close]

BLAST of CmoCh19G000660 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 45.8 bits (107), Expect = 1.7e-05
Identity = 23/61 (37.70%), Postives = 36/61 (59.02%), Query Frame = 0


Query: 12 WPELVGMDGEKAVKIIQQDNPSLYVILMPRGEEAASANFMFKRVRVFVDYCGRVNSIPRI 71
          WPEL G +G+ A  +I+++NP++   ++  G    +A+F   RVRVFVD    V   P+ 
Sbjct: 11 WPELTGTNGDYAAVVIERENPTVNAAVILDG-SPVTADFRCDRVRVFVDGNRIVVKTPKS 70

Query: 72 G 73
          G
Sbjct: 71 G 70

The following BLAST results are available for this feature:

BLAST of CmoCh19G000660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P01053	7.8e-08	40.63	Subtilisin-chymotrypsin inhibitor-2A OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]
P82977	2.3e-07	35.82	Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2	[more]
P08626	3.9e-07	38.46	Subtilisin-chymotrypsin inhibitor-2B (Fragment) OS=Hordeum vulgare OX=4513 PE=2 ...	[more]
Q6XNP7	1.6e-05	42.62	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
P16231	8.1e-05	38.33	Wound-induced proteinase inhibitor 1 OS=Solanum peruvianum OX=4082 PE=3 SV=1	[more]

BLAST of CmoCh19G000660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
Q43421	8.1e-16	69.23	Pumpkin fruit trypsin inhibitor OS=Cucurbita maxima OX=3661 GN=pfiAF4 PE=2 SV=1	[more]
Q6DKU8	8.1e-16	69.23	Putative chymotrypsin protease inhibitor OS=Cucurbita maxima OX=3661 PE=3 SV=1	[more]
Q43420	4.0e-15	69.23	Pumpkin Fruit Chymotrypsin Inhibitor OS=Cucurbita maxima OX=3661 GN=pfiBM7 PE=2 ...	[more]
A0A2G9GNE2	1.4e-07	50.79	Uncharacterized protein OS=Handroanthus impetiginosus OX=429701 GN=CDL12_20721 P...	[more]
A0A1R3I780	3.1e-07	43.06	Proteinase inhibitor I13, potato inhibitor I OS=Corchorus capsularis OX=210143 G...	[more]

BLAST of CmoCh19G000660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G46860.1	4.7e-08	40.00	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	4.0e-07	36.62	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G50020.1	1.3e-05	39.06	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT2G38870.1	1.7e-05	37.70	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 9..72 e-value: 1.6E-18 score: 67.1
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 8..72
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PROSITE	PS00285	POTATO_INHIBITOR	coord: 12..23
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 5..72 e-value: 1.4E-20 score: 75.5
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 5..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh19G000660.1	CmoCh19G000660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity