CmoCh18G013030 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G013030
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionProtein of unknown function (DUF3464)
LocationCmo_Chr18: 12496259 .. 12496828 (-)
RNA-Seq ExpressionCmoCh18G013030
SyntenyCmoCh18G013030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGCTCTGGTGTGTTCCTTCCGGCACGCTCTACACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCGATGACTCAACGACCACGAAGAATCTCAGAATTTTCACAAGAACATCCCGAGGCCAGATCCAACTAAACGCCAAGGGTTTCACAGGTTCTCCGGCAACAGCGAAAGACAGAGAAACCGCCGTGAAAAAACCTTCCGGCCAAAACAATAGCAGAGACGGCGGCGGCGACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTTGGAGTTCCGATGGCGTTCGGCGTGACATTGGTGAAGATCTTAGAGGCGGTCCATGAGCAGAAACTTTGGCATGTGCCAATTTGGCTGCCGTTCTTGACGATTTTCTTGACATTTGGAGCTTCGACGATGGGAATCGCTTACGGGACGCTGTCGACGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGATGAGGCGCAGAAGAATTGGGTTGAGATGTGGAAGGAGGAAGACGAAGCCAATCGTGGAGGAAACTAA

mRNA sequence

ATGAAAGCTCTGGTGTGTTCCTTCCGGCACGCTCTACACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCGATGACTCAACGACCACGAAGAATCTCAGAATTTTCACAAGAACATCCCGAGGCCAGATCCAACTAAACGCCAAGGGTTTCACAGGTTCTCCGGCAACAGCGAAAGACAGAGAAACCGCCGTGAAAAAACCTTCCGGCCAAAACAATAGCAGAGACGGCGGCGGCGACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTTGGAGTTCCGATGGCGTTCGGCGTGACATTGGTGAAGATCTTAGAGGCGGTCCATGAGCAGAAACTTTGGCATGTGCCAATTTGGCTGCCGTTCTTGACGATTTTCTTGACATTTGGAGCTTCGACGATGGGAATCGCTTACGGGACGCTGTCGACGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGATGAGGCGCAGAAGAATTGGGTTGAGATGTGGAAGGAGGAAGACGAAGCCAATCGTGGAGGAAACTAA

Coding sequence (CDS)

ATGAAAGCTCTGGTGTGTTCCTTCCGGCACGCTCTACACCTCTCGAAGCCTCCGCCATGGAAACAGAGAAGCCGAATCGATGACTCAACGACCACGAAGAATCTCAGAATTTTCACAAGAACATCCCGAGGCCAGATCCAACTAAACGCCAAGGGTTTCACAGGTTCTCCGGCAACAGCGAAAGACAGAGAAACCGCCGTGAAAAAACCTTCCGGCCAAAACAATAGCAGAGACGGCGGCGGCGACGACGATGAAATTCCAGAGGCCGTAGCCAAGAGGATCATAACGAGAATCTTGGCTTTCGTTGGAGTTCCGATGGCGTTCGGCGTGACATTGGTGAAGATCTTAGAGGCGGTCCATGAGCAGAAACTTTGGCATGTGCCAATTTGGCTGCCGTTCTTGACGATTTTCTTGACATTTGGAGCTTCGACGATGGGAATCGCTTACGGGACGCTGTCGACGAGTTTGGATCCGGAGAAGGAAGGGACTCTGTTTGGATGGGATGAGGCGCAGAAGAATTGGGTTGAGATGTGGAAGGAGGAAGACGAAGCCAATCGTGGAGGAAACTAA

Protein sequence

MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATAKDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVHEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKEEDEANRGGN
Homology
BLAST of CmoCh18G013030 vs. ExPASy Swiss-Prot
Match: Q9LTD9 (Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 69/185 (37.30%), Postives = 106/185 (57.30%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSR----GQIQLNAKGFTGS 60
           M+AL+CS R         P    SR    T + N +     ++     ++    KGF  S
Sbjct: 1   MRALLCSHRLL-------PLSSLSRTTVKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSS 60

Query: 61  PATAKDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKIL 120
             +        +KP   +   +   DD  IP+ V +R++ RI+  VG P+  GV ++K+L
Sbjct: 61  RTS--------EKPGRPDPDPE---DDPPIPQEVFERMMGRIVVSVGTPLGLGVAILKVL 120

Query: 121 EAVHEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVE 180
           E + ++ +W VP+W+P+LT  +TFG+S +GIAYG+LST+LDP K  +LFG  EA++NWVE
Sbjct: 121 EVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLSTNLDPAKTNSLFGLKEAKENWVE 167

Query: 181 MWKEE 182
           MWKE+
Sbjct: 181 MWKED 167

BLAST of CmoCh18G013030 vs. ExPASy Swiss-Prot
Match: O49668 (Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.7e-17
Identity = 55/171 (32.16%), Postives = 89/171 (52.05%), Query Frame = 0

Query: 21  KQRSRIDDSTTTKNLRIFTRTSRGQIQLNA-KGFTGSPATAKDRETAVKKPSGQNNSRDG 80
           K +S++  S T  N    +R +     +N+ KGF   P   K +++   KP  Q++  D 
Sbjct: 37  KTKSQVPKSITCINRLEISRIAPLHATMNSPKGF--GPPPKKTKKSKKPKPGNQSDEDDD 96

Query: 81  GGDDDE-----------IPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVHEQKLWHVP 140
             D+D+           IPE V  R+I+R+   VG+P+  G+        +       VP
Sbjct: 97  DEDEDDDDEEDERERGVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVP 156

Query: 141 IWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180
            W+PF+  F+ FG +  G++YG +S+S DP +EG+L GW+EA+KNW   W+
Sbjct: 157 TWVPFIVSFVFFGTALAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQ 205

BLAST of CmoCh18G013030 vs. ExPASy TrEMBL
Match: A0A6J1FZC0 (uncharacterized protein PAM68-like OS=Cucurbita moschata OX=3662 GN=LOC111449293 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 7.9e-103
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA 60
           MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA
Sbjct: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVH 120
           KDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVH
Sbjct: 61  KDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVH 120

Query: 121 EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE 180
           EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE
Sbjct: 121 EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE 180

Query: 181 EDEANRGGN 190
           EDEANRGGN
Sbjct: 181 EDEANRGGN 189

BLAST of CmoCh18G013030 vs. ExPASy TrEMBL
Match: A0A6J1HYL3 (uncharacterized protein PAM68-like OS=Cucurbita maxima OX=3661 GN=LOC111467790 PE=4 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 1.6e-95
Identity = 180/189 (95.24%), Postives = 184/189 (97.35%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA 60
           MKALVCSFRHAL LSKPPPWKQRSRID+STTTKNLR+FT TSRGQIQLNAKGFTGSPATA
Sbjct: 1   MKALVCSFRHALDLSKPPPWKQRSRIDNSTTTKNLRVFTGTSRGQIQLNAKGFTGSPATA 60

Query: 61  KDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVH 120
           KDRETAVKK S QNNSRD GGDDDEIPEAVAKRIITRILAFVG+PMAFGVTLVKILEAVH
Sbjct: 61  KDRETAVKKLSDQNNSRD-GGDDDEIPEAVAKRIITRILAFVGIPMAFGVTLVKILEAVH 120

Query: 121 EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE 180
           EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE
Sbjct: 121 EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE 180

Query: 181 EDEANRGGN 190
           EDEANRGG+
Sbjct: 181 EDEANRGGD 188

BLAST of CmoCh18G013030 vs. ExPASy TrEMBL
Match: A0A6J1CGP3 (uncharacterized protein PAM68-like OS=Momordica charantia OX=3673 GN=LOC111011500 PE=4 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 5.9e-74
Identity = 141/186 (75.81%), Postives = 161/186 (86.56%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPATA 60
           MKAL+CS+R ALHLS+PPPWK RSRI+DST TKNL   +R +RG +++NAKGFTG+PATA
Sbjct: 1   MKALLCSYRQALHLSEPPPWKPRSRINDSTITKNLTNLSRIARGDVRVNAKGFTGTPATA 60

Query: 61  KDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVH 120
           K+RE A +K SGQNNS     DDDEI EAV+ RIITRILAFVGVPMAFGV L+KI E + 
Sbjct: 61  KEREAAARKISGQNNS--SNDDDDEISEAVSNRIITRILAFVGVPMAFGVALLKIFETIK 120

Query: 121 EQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWKE 180
           EQK+W VPIWLPFLT FLTFGAST+GIAYGTLS SLDPEKEG+LFGW+EAQKNWVEMWKE
Sbjct: 121 EQKVWDVPIWLPFLTTFLTFGASTIGIAYGTLSASLDPEKEGSLFGWEEAQKNWVEMWKE 180

Query: 181 EDEANR 187
           EDEA+R
Sbjct: 181 EDEASR 184

BLAST of CmoCh18G013030 vs. ExPASy TrEMBL
Match: A0A5A7STB5 (Putative Sterol regulatory element-binding protein cleavage-activating protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001970 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.9e-70
Identity = 140/187 (74.87%), Postives = 157/187 (83.96%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRS-RIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPAT 60
           MK+  CSFR AL L KPPP  QR+  I+DST TKNL  FT TSRGQ+Q+NAKGFTGSPAT
Sbjct: 1   MKSFGCSFRQALLLPKPPPRNQRTVIINDSTITKNLSNFTTTSRGQVQVNAKGFTGSPAT 60

Query: 61  AKDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAV 120
           AK+RETA K  SGQNN+ D   DDDEIPEAV  RIITRILAFVG+PMAFGVTL+KI +A+
Sbjct: 61  AKNRETAAKNLSGQNNNED---DDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAI 120

Query: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180
            EQ LW VPIW+PF T FLTFGASTMGIAYGTLSTSLDPEK+G++ GW+EAQKNWVEMWK
Sbjct: 121 KEQNLWDVPIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVMGWEEAQKNWVEMWK 180

Query: 181 EEDEANR 187
           EEDE +R
Sbjct: 181 EEDEVSR 184

BLAST of CmoCh18G013030 vs. ExPASy TrEMBL
Match: A0A1S3AZ57 (uncharacterized protein PAM68-like OS=Cucumis melo OX=3656 GN=LOC103484134 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 3.9e-70
Identity = 140/187 (74.87%), Postives = 157/187 (83.96%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRS-RIDDSTTTKNLRIFTRTSRGQIQLNAKGFTGSPAT 60
           MK+  CSFR AL L KPPP  QR+  I+DST TKNL  FT TSRGQ+Q+NAKGFTGSPAT
Sbjct: 1   MKSFGCSFRQALLLPKPPPRNQRTVIINDSTITKNLSNFTTTSRGQVQVNAKGFTGSPAT 60

Query: 61  AKDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKILEAV 120
           AK+RETA K  SGQNN+ D   DDDEIPEAV  RIITRILAFVG+PMAFGVTL+KI +A+
Sbjct: 61  AKNRETAAKNLSGQNNNED---DDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAI 120

Query: 121 HEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180
            EQ LW VPIW+PF T FLTFGASTMGIAYGTLSTSLDPEK+G++ GW+EAQKNWVEMWK
Sbjct: 121 KEQNLWDVPIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVMGWEEAQKNWVEMWK 180

Query: 181 EEDEANR 187
           EEDE +R
Sbjct: 181 EEDEVSR 184

BLAST of CmoCh18G013030 vs. TAIR 10
Match: AT5G52780.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 69/185 (37.30%), Postives = 106/185 (57.30%), Query Frame = 0

Query: 1   MKALVCSFRHALHLSKPPPWKQRSRIDDSTTTKNLRIFTRTSR----GQIQLNAKGFTGS 60
           M+AL+CS R         P    SR    T + N +     ++     ++    KGF  S
Sbjct: 1   MRALLCSHRLL-------PLSSLSRTTVKTKSHNPKTLYPNNKPRWESKLHAGPKGFQSS 60

Query: 61  PATAKDRETAVKKPSGQNNSRDGGGDDDEIPEAVAKRIITRILAFVGVPMAFGVTLVKIL 120
             +        +KP   +   +   DD  IP+ V +R++ RI+  VG P+  GV ++K+L
Sbjct: 61  RTS--------EKPGRPDPDPE---DDPPIPQEVFERMMGRIVVSVGTPLGLGVAILKVL 120

Query: 121 EAVHEQKLWHVPIWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVE 180
           E + ++ +W VP+W+P+LT  +TFG+S +GIAYG+LST+LDP K  +LFG  EA++NWVE
Sbjct: 121 EVLKDRNVWDVPLWVPYLTTLVTFGSSALGIAYGSLSTNLDPAKTNSLFGLKEAKENWVE 167

Query: 181 MWKEE 182
           MWKE+
Sbjct: 181 MWKED 167

BLAST of CmoCh18G013030 vs. TAIR 10
Match: AT4G19100.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 89.7 bits (221), Expect = 2.7e-18
Identity = 55/171 (32.16%), Postives = 89/171 (52.05%), Query Frame = 0

Query: 21  KQRSRIDDSTTTKNLRIFTRTSRGQIQLNA-KGFTGSPATAKDRETAVKKPSGQNNSRDG 80
           K +S++  S T  N    +R +     +N+ KGF   P   K +++   KP  Q++  D 
Sbjct: 37  KTKSQVPKSITCINRLEISRIAPLHATMNSPKGF--GPPPKKTKKSKKPKPGNQSDEDDD 96

Query: 81  GGDDDE-----------IPEAVAKRIITRILAFVGVPMAFGVTLVKILEAVHEQKLWHVP 140
             D+D+           IPE V  R+I+R+   VG+P+  G+        +       VP
Sbjct: 97  DEDEDDDDEEDERERGVIPEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVP 156

Query: 141 IWLPFLTIFLTFGASTMGIAYGTLSTSLDPEKEGTLFGWDEAQKNWVEMWK 180
            W+PF+  F+ FG +  G++YG +S+S DP +EG+L GW+EA+KNW   W+
Sbjct: 157 TWVPFIVSFVFFGTALAGVSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQ 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTD91.5e-2637.30Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 ... [more]
O496683.7e-1732.16Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FZC07.9e-103100.00uncharacterized protein PAM68-like OS=Cucurbita moschata OX=3662 GN=LOC111449293... [more]
A0A6J1HYL31.6e-9595.24uncharacterized protein PAM68-like OS=Cucurbita maxima OX=3661 GN=LOC111467790 P... [more]
A0A6J1CGP35.9e-7475.81uncharacterized protein PAM68-like OS=Momordica charantia OX=3673 GN=LOC11101150... [more]
A0A5A7STB53.9e-7074.87Putative Sterol regulatory element-binding protein cleavage-activating protein O... [more]
A0A1S3AZ573.9e-7074.87uncharacterized protein PAM68-like OS=Cucumis melo OX=3656 GN=LOC103484134 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT5G52780.11.1e-2737.30Protein of unknown function (DUF3464) [more]
AT4G19100.12.7e-1832.16Protein of unknown function (DUF3464) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021855PAM68-likePFAMPF11947DUF3464coord: 60..180
e-value: 2.7E-36
score: 124.6
IPR021855PAM68-likePANTHERPTHR34575PROTEIN PAM68, CHLOROPLASTICcoord: 1..185
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 51..85
NoneNo IPR availablePANTHERPTHR34575:SF6EXPRESSED PROTEINcoord: 1..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G013030.1CmoCh18G013030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane