CmoCh18G008170 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh18G008170
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein ASPARTIC PROTEASE IN GUARD CELL 2-like
LocationCmo_Chr18: 9584181 .. 9585863 (+)
RNA-Seq ExpressionCmoCh18G008170
SyntenyCmoCh18G008170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTCCTAAAACTTCACTTTTCTCTTCTTCTCTTCTTCTCGGTCTCTGTCGTCTTTGGCAGCCGTTCTTCTCCGGTCGAGTTTCAATATCTAAATGTCAAATCGAGCAAGTTCGATGCTAATGATAGTCAAATTCTTCATATCCTTAACTTCTCCAACGGTCACCGGTTGGTGACGGGTCGAAAATCTAACCATACTAAATTTAAGCTCAAACTTGTTCATCGAGACGAGCTCCCTCACATCCATGGTCAACATCAGGGCTTCGATGAGCGTATCAAAAGAGATGTCAAACGAGTTGCCACCCTTGTCCGCCGTCTATCTCGTTATGACACTGATAGCTCCGGCGTTCAAGATAATAATTACAAGGTGGCGAATTTTGGGACGGGTGTCATTTCAGGTGAGAGAATCTTTTTTTTAAATAATACCCGTTAAAATCCAATATTTACATTTATGATTTTTTTTTTTCCATTTTTCGAATGATTTGGAATTAGGGATGGAAGAGGGGAATGGAGAGTATTTTGTTCGGATAGGAGTTGGGAGCCCGCCGAGGAGTCAATACATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGTCAACCTTGTAGGCAGTGCTATCAACAGTCCGATCCCGTTTTCAACCCGGCCGAGTCCACTTCGTATACCGGTGTCTCATGTGGCTCGGCTGTTTGCGACCGCCTCGAGAACGCTGGCTGCCATGCTGGACGGTGTCGATATGAGGTGTCGTATGGCGATGGGTCTTACACTAAAGGCACTCTCGCCCTCGAAACTCTCACTATTGGCGAGGTTAGTACGCATATAGATTATGATATATTTAAAGGTCGATTTTATATACAATAAATTATCATGAATTTCATTAAATAAATGTAAATAGTATTTATAAGCTTAATTTTAAAAATCAAATGATTATTAAACTTGATATTCTTGACTAGGTTATGATTCATGACATGGCTATTGGGTGCGGTCATACCAACCAAGGGATGTTCATTGGAGCTGCCGGGCTACTCGGTCTCGGTGGTGGTTCGATGTCATTTGTTGGCCAACTTGGCGGTCAGACCGGTGGTGCATTTAGCTACTGTTTAGTGAGCCGAGGTATCGGCTCGACCGGGACATTAGAATTTGGCCGCGGAGCAATGCCGGTTGGTGCCACGTGGATCTCCTTGGTTCGAAACCCAAATGCCCCAAGCTTCTACTACATCGGACTAGCCGGCGTCGGTGTCGGAGGCATCAAAGTTCCAATACCCGAAGAAACTTTCCAGCTGACGGAGTACGGAACCAACGGCGTCGTAATGGACACCGGCACCGCCGTAACACGGCTACCAACAGCAGCCTACGTGGCATTCCGCGATTCGTTCACAGCCCAAACCAGCAACCTACCACGAGCGCCCGGAGTTTCGATTTTCGACACGTGTTATAATCTCAACGGCTTCGAGTCCGTACGAGTGCCAACCGTGTCGTTTTACTTCTCGGACGGCCCGGTGCTGACGCTTCCGGCGAATAACTTCCTGATTCCGGTTGACAGCGCCGGAACTTTCTGCTTCGCTTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAACAGGGAGGGATTCAAATTTCATTTGATGGGGCTAATGGGTTTGTTGGATTTGGCCCAAATATTTGCTAA

mRNA sequence

ATGCTCCTAAAACTTCACTTTTCTCTTCTTCTCTTCTTCTCGGTCTCTGTCGTCTTTGGCAGCCGTTCTTCTCCGGTCGAGTTTCAATATCTAAATGTCAAATCGAGCAAGTTCGATGCTAATGATAGTCAAATTCTTCATATCCTTAACTTCTCCAACGGTCACCGGTTGGTGACGGGTCGAAAATCTAACCATACTAAATTTAAGCTCAAACTTGTTCATCGAGACGAGCTCCCTCACATCCATGGTCAACATCAGGGCTTCGATGAGCGTATCAAAAGAGATGTCAAACGAGTTGCCACCCTTGTCCGCCGTCTATCTCGTTATGACACTGATAGCTCCGGCGTTCAAGATAATAATTACAAGGTGGCGAATTTTGGGACGGGTGTCATTTCAGGGATGGAAGAGGGGAATGGAGAGTATTTTGTTCGGATAGGAGTTGGGAGCCCGCCGAGGAGTCAATACATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGTCAACCTTGTAGGCAGTGCTATCAACAGTCCGATCCCGTTTTCAACCCGGCCGAGTCCACTTCGTATACCGGTGTCTCATGTGGCTCGGCTGTTTGCGACCGCCTCGAGAACGCTGGCTGCCATGCTGGACGGTGTCGATATGAGGTGTCGTATGGCGATGGGTCTTACACTAAAGGCACTCTCGCCCTCGAAACTCTCACTATTGGCGAGGTTATGATTCATGACATGGCTATTGGGTGCGGTCATACCAACCAAGGGATGTTCATTGGAGCTGCCGGGCTACTCGGTCTCGGTGGTGGTTCGATGTCATTTGTTGGCCAACTTGGCGGTCAGACCGGTGGTGCATTTAGCTACTGTTTAGTGAGCCGAGGTATCGGCTCGACCGGGACATTAGAATTTGGCCGCGGAGCAATGCCGGTTGGTGCCACGTGGATCTCCTTGGTTCGAAACCCAAATGCCCCAAGCTTCTACTACATCGGACTAGCCGGCGTCGGTGTCGGAGGCATCAAAGTTCCAATACCCGAAGAAACTTTCCAGCTGACGGAGTACGGAACCAACGGCGTCGTAATGGACACCGGCACCGCCGTAACACGGCTACCAACAGCAGCCTACGTGGCATTCCGCGATTCGTTCACAGCCCAAACCAGCAACCTACCACGAGCGCCCGGAGTTTCGATTTTCGACACGTGTTATAATCTCAACGGCTTCGAGTCCGTACGAGTGCCAACCGTGTCGTTTTACTTCTCGGACGGCCCGGTGCTGACGCTTCCGGCGAATAACTTCCTGATTCCGGTTGACAGCGCCGGAACTTTCTGCTTCGCTTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAACAGGGAGGGATTCAAATTTCATTTGATGGGGCTAATGGGTTTGTTGGATTTGGCCCAAATATTTGCTAA

Coding sequence (CDS)

ATGCTCCTAAAACTTCACTTTTCTCTTCTTCTCTTCTTCTCGGTCTCTGTCGTCTTTGGCAGCCGTTCTTCTCCGGTCGAGTTTCAATATCTAAATGTCAAATCGAGCAAGTTCGATGCTAATGATAGTCAAATTCTTCATATCCTTAACTTCTCCAACGGTCACCGGTTGGTGACGGGTCGAAAATCTAACCATACTAAATTTAAGCTCAAACTTGTTCATCGAGACGAGCTCCCTCACATCCATGGTCAACATCAGGGCTTCGATGAGCGTATCAAAAGAGATGTCAAACGAGTTGCCACCCTTGTCCGCCGTCTATCTCGTTATGACACTGATAGCTCCGGCGTTCAAGATAATAATTACAAGGTGGCGAATTTTGGGACGGGTGTCATTTCAGGGATGGAAGAGGGGAATGGAGAGTATTTTGTTCGGATAGGAGTTGGGAGCCCGCCGAGGAGTCAATACATGGTGATTGATTCCGGCAGTGACATTGTGTGGGTTCAATGTCAACCTTGTAGGCAGTGCTATCAACAGTCCGATCCCGTTTTCAACCCGGCCGAGTCCACTTCGTATACCGGTGTCTCATGTGGCTCGGCTGTTTGCGACCGCCTCGAGAACGCTGGCTGCCATGCTGGACGGTGTCGATATGAGGTGTCGTATGGCGATGGGTCTTACACTAAAGGCACTCTCGCCCTCGAAACTCTCACTATTGGCGAGGTTATGATTCATGACATGGCTATTGGGTGCGGTCATACCAACCAAGGGATGTTCATTGGAGCTGCCGGGCTACTCGGTCTCGGTGGTGGTTCGATGTCATTTGTTGGCCAACTTGGCGGTCAGACCGGTGGTGCATTTAGCTACTGTTTAGTGAGCCGAGGTATCGGCTCGACCGGGACATTAGAATTTGGCCGCGGAGCAATGCCGGTTGGTGCCACGTGGATCTCCTTGGTTCGAAACCCAAATGCCCCAAGCTTCTACTACATCGGACTAGCCGGCGTCGGTGTCGGAGGCATCAAAGTTCCAATACCCGAAGAAACTTTCCAGCTGACGGAGTACGGAACCAACGGCGTCGTAATGGACACCGGCACCGCCGTAACACGGCTACCAACAGCAGCCTACGTGGCATTCCGCGATTCGTTCACAGCCCAAACCAGCAACCTACCACGAGCGCCCGGAGTTTCGATTTTCGACACGTGTTATAATCTCAACGGCTTCGAGTCCGTACGAGTGCCAACCGTGTCGTTTTACTTCTCGGACGGCCCGGTGCTGACGCTTCCGGCGAATAACTTCCTGATTCCGGTTGACAGCGCCGGAACTTTCTGCTTCGCTTTTGCTCCGTCGCCGTCGGGGCTTTCCATAATCGGAAACATCCAACAGGGAGGGATTCAAATTTCATTTGATGGGGCTAATGGGTTTGTTGGATTTGGCCCAAATATTTGCTAA

Protein sequence

MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC
Homology
BLAST of CmoCh18G008170 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 3.7e-178
Identity = 310/482 (64.32%), Postives = 380/482 (78.84%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60
           MLL L F   L   + +   S  S  +FQ ++V         +    + +F+N H     
Sbjct: 1   MLLPLFF-FFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTAT----LPDFNNTH----F 60

Query: 61  RKSNHTKFKLKLVHRDELPHI--HGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120
              + +K+ L+L+HRD  P +     H     R++RD  RV+ ++RR+S     SS   D
Sbjct: 61  SDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSS---D 120

Query: 121 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180
           + Y+V +FG+ ++SGM++G+GEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC+ CY+Q
Sbjct: 121 SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQ 180

Query: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S SYTGVSCGS+VCDR+EN+GCH+G CRYEV YGDGSYTKGTLALETLT  
Sbjct: 181 SDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFA 240

Query: 241 EVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300
           + ++ ++A+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRG  STG
Sbjct: 241 KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360
           +L FGR A+PVGA+W+ LVRNP APSFYY+GL G+GVGG+++P+P+  F LTE G  GVV
Sbjct: 301 SLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVV 360

Query: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAYVAFRD F +QT+NLPRA GVSIFDTCY+L+GF SVRVPTVSFYF+
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFT 420

Query: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480
           +GPVLTLPA NFL+PVD +GT+CFAFA SP+GLSIIGNIQQ GIQ+SFDGANGFVGFGPN
Sbjct: 421 EGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPN 470

BLAST of CmoCh18G008170 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 1.4e-105
Identity = 194/431 (45.01%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 63  SNHTKFKLKLVHRDELPHIHGQHQGFD----ERIKRDVKRVATLVRRL-------SRYDT 122
           ++ +   L+L  RD    +  QH+ +      R++RD  RVA +V ++        R D 
Sbjct: 75  NSSSPLSLELHSRDTF--VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDL 134

Query: 123 DSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQP 182
                +D  Y+  +  T V+SG  +G+GEYF RIGVG+P +  Y+V+D+GSD+ W+QC+P
Sbjct: 135 KPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP 194

Query: 183 CRQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLA 242
           C  CYQQSDPVFNP  S++Y  ++C +  C  LE + C + +C Y+VSYGDGS+T G LA
Sbjct: 195 CADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELA 254

Query: 243 LETLTIGEV-MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLV 302
            +T+T G    I+++A+GCGH N+G+F GAAGLLGLGGG +S   Q+      +FSYCLV
Sbjct: 255 TDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSFSYCLV 314

Query: 303 SRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLT 362
            R  G + +L+F    +  G     L+RN    +FYY+GL+G  VGG KV +P+  F + 
Sbjct: 315 DRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVD 374

Query: 363 EYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPR-APGVSIFDTCYNLNGFESVR 422
             G+ GV++D GTAVTRL T AY + RD+F   T NL + +  +S+FDTCY+ +   +V+
Sbjct: 375 ASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVK 434

Query: 423 VPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGA 481
           VPTV+F+F+ G  L LPA N+LIPVD +GTFCFAFAP+ S LSIIGN+QQ G +I++D +
Sbjct: 435 VPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLS 494

BLAST of CmoCh18G008170 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.3e-103
Identity = 206/465 (44.30%), Postives = 284/465 (61.08%), Query Frame = 0

Query: 23  SSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKSNHTKFKLKLVHRDELPHIH 82
           +SPV FQ           +DS+ L    F +G         + +   L L H D L    
Sbjct: 41  ASPVSFQ---------PDSDSESLLESEFESG-----SDSESSSSITLNLDHIDALSSNK 100

Query: 83  GQHQGFDERIKRD---VKRVATLVRRLSRYDTDSSGVQDNNYKVANFGTGVISGMEEGNG 142
              + F  R++RD   VK +ATL  ++   +   +       +   F + V+SG+ +G+G
Sbjct: 101 TPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP------RPGGFSSSVVSGLSQGSG 160

Query: 143 EYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFNPAESTSYTGVSCGSA 202
           EYF R+GVG+P R  YMV+D+GSDIVW+QC PCR+CY QSDP+F+P +S +Y  + C S 
Sbjct: 161 EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSP 220

Query: 203 VCDRLENAGCHAGR--CRYEVSYGDGSYTKGTLALETLTIGEVMIHDMAIGCGHTNQGMF 262
            C RL++AGC+  R  C Y+VSYGDGS+T G  + ETLT     +  +A+GCGH N+G+F
Sbjct: 221 HCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF 280

Query: 263 IGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGS-TGTLEFGRGAMPVGATWISL 322
           +GAAGLLGLG G +SF GQ G +    FSYCLV R   S   ++ FG  A+   A +  L
Sbjct: 281 VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPL 340

Query: 323 VRNPNAPSFYYIGLAGVGVGGIKVP-IPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVA 382
           + NP   +FYY+GL G+ VGG +VP +    F+L + G  GV++D+GT+VTRL   AY+A
Sbjct: 341 LSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIA 400

Query: 383 FRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVD 442
            RD+F      L RAP  S+FDTC++L+    V+VPTV  +F  G  ++LPA N+LIPVD
Sbjct: 401 MRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVD 460

Query: 443 SAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
           + G FCFAFA +  GLSIIGNIQQ G ++ +D A+  VGF P  C
Sbjct: 461 TNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of CmoCh18G008170 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 4.6e-72
Identity = 171/456 (37.50%), Postives = 241/456 (52.85%), Query Frame = 0

Query: 39  DANDSQILHILNFSNGHRLVTGRKSNHTKFKLKLVHR----DELPHIHGQHQGFDERIKR 98
           D++  Q+  +L  S+   +++ R S  TK  L + HR      L +         E ++ 
Sbjct: 32  DSHTIQVSSLLPSSSSSCVLSPRAST-TKSSLHVTHRHGTCSRLNNGKATSPDHVEILRL 91

Query: 99  DVKRVATLVRRLS-RYDTDSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRS 158
           D  RV ++  +LS +  TD      +    A  G+ +      G+G Y V +G+G+P   
Sbjct: 92  DQARVNSIHSKLSKKLATDHVSESKSTDLPAKDGSTL------GSGNYIVTVGLGTPKND 151

Query: 159 QYMVIDSGSDIVWVQCQPC-RQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENA----- 218
             ++ D+GSD+ W QCQPC R CY Q +P+FNP++STSY  VSC SA C  L +A     
Sbjct: 152 LSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAG 211

Query: 219 GCHAGRCRYEVSYGDGSYTKGTLALETLTIGEVMIHD-MAIGCGHTNQGMFIGAAGLLGL 278
            C A  C Y + YGD S++ G LA E  T+    + D +  GCG  NQG+F G AGLLGL
Sbjct: 212 SCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGL 271

Query: 279 GGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFY 338
           G   +SF  Q        FSYCL S     TG L FG   +     +  +    +  SFY
Sbjct: 272 GRDKLSFPSQTATAYNKIFSYCLPS-SASYTGHLTFGSAGISRSVKFTPISTITDGTSFY 331

Query: 339 YIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSN 398
            + +  + VGG K+PIP      T + T G ++D+GT +TRLP  AY A R SF A+ S 
Sbjct: 332 GLNIVAITVGGQKLPIPS-----TVFSTPGALIDSGTVITRLPPKAYAALRSSFKAKMSK 391

Query: 399 LPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFA- 458
            P   GVSI DTC++L+GF++V +P V+F FS G V+ L +   +  V      C AFA 
Sbjct: 392 YPTTSGVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKG-IFYVFKISQVCLAFAG 451

Query: 459 -PSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
               S  +I GN+QQ  +++ +DGA G VGF PN C
Sbjct: 452 NSDDSNAAIFGNVQQQTLEVVYDGAGGRVGFAPNGC 473

BLAST of CmoCh18G008170 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.2e-69
Identity = 159/452 (35.18%), Postives = 233/452 (51.55%), Query Frame = 0

Query: 34  KSSKFDANDSQILHILN-FSNGHRLVTGRKSNHTKFKLKLVH-RDELPHIHGQHQ-GFDE 93
           KS      DS  + + + F +    V   K+++TK  L++VH      H+    +   DE
Sbjct: 28  KSDSGKVLDSYTIQVSSLFPSSSSCVPSSKASNTKSSLRVVHMHGACSHLSSDARVDHDE 87

Query: 94  RIKRDVKRVATLVRRLSRYDTDSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSP 153
            I+RD  RV ++  +LS+   +      +    A       SG+  G+G Y V IG+G+P
Sbjct: 88  IIRRDQARVESIYSKLSKNSANEVSEAKSTELPAK------SGITLGSGNYIVTIGIGTP 147

Query: 154 PRSQYMVIDSGSDIVWVQCQPC-RQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENAGC 213
                +V D+GSD+ W QC+PC   CY Q +P FNP+ S++Y  VSC S +C+  E+  C
Sbjct: 148 KHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES--C 207

Query: 214 HAGRCRYEVSYGDGSYTKGTLALETLTI-GEVMIHDMAIGCGHTNQGMFIGAAGLLGLGG 273
            A  C Y + YGD S+T+G LA E  T+    ++ D+  GCG  NQG+F G AGLLGLG 
Sbjct: 208 SASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGP 267

Query: 274 GSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFYYI 333
           G +S   Q        FSYCL S    STG L FG   +     +  +   P+A + Y I
Sbjct: 268 GKLSLPAQTTTTYNNIFSYCLPSFTSNSTGHLTFGSAGISESVKFTPISSFPSAFN-YGI 327

Query: 334 GLAGVGVGGIKVPIPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLP 393
            + G+ VG  ++ I   +F      T G ++D+GT  TRLPT  Y   R  F  + S+  
Sbjct: 328 DIIGISVGDKELAITPNSF-----STEGAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYK 387

Query: 394 RAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFAPSP 453
              G  +FDTCY+  G ++V  PT++F F+   V+ L  +   +P+      C AFA + 
Sbjct: 388 STSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPI-KISQVCLAFAGND 447

Query: 454 SGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
              +I GN+QQ  + + +D A G VGF PN C
Sbjct: 448 DLPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

BLAST of CmoCh18G008170 vs. ExPASy TrEMBL
Match: A0A6J1G1D0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449773 PE=4 SV=1)

HSP 1 Score: 970.7 bits (2508), Expect = 2.2e-279
Identity = 480/480 (100.00%), Postives = 480/480 (100.00%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60
           MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG
Sbjct: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60

Query: 61  RKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNN 120
           RKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNN
Sbjct: 61  RKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNN 120

Query: 121 YKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD 180
           YKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD
Sbjct: 121 YKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD 180

Query: 181 PVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEV 240
           PVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEV
Sbjct: 181 PVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEV 240

Query: 241 MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL 300
           MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL
Sbjct: 241 MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL 300

Query: 301 EFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD 360
           EFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD
Sbjct: 301 EFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD 360

Query: 361 TGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG 420
           TGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG
Sbjct: 361 TGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG 420

Query: 421 PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 480
           PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC
Sbjct: 421 PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 480

BLAST of CmoCh18G008170 vs. ExPASy TrEMBL
Match: A0A6J1HQG0 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111466434 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.0e-267
Identity = 459/480 (95.62%), Postives = 470/480 (97.92%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60
           MLLKLHFSLLLFFSVSVV GSRSS VEFQYLNVKS+KFD NDSQ+LH LNFSNGHRLVTG
Sbjct: 1   MLLKLHFSLLLFFSVSVVLGSRSSSVEFQYLNVKSNKFDVNDSQLLHTLNFSNGHRLVTG 60

Query: 61  RKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNN 120
           RKSNHTKFKLKLVHRD+LPHIHGQHQGFDERIKRDVKRVATLVRRLSR DT +S V+DN+
Sbjct: 61  RKSNHTKFKLKLVHRDKLPHIHGQHQGFDERIKRDVKRVATLVRRLSRDDTTTSVVEDNS 120

Query: 121 YKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD 180
           YKVANFGTGVISGMEEG+GEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD
Sbjct: 121 YKVANFGTGVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSD 180

Query: 181 PVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEV 240
           PVFNPAESTSYTGVSCGSA+CDRLENAGCH+GRCRYEVSYGDGSYTKGTLALETLTIGEV
Sbjct: 181 PVFNPAESTSYTGVSCGSAICDRLENAGCHSGRCRYEVSYGDGSYTKGTLALETLTIGEV 240

Query: 241 MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL 300
            + DMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL
Sbjct: 241 TVRDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTL 300

Query: 301 EFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD 360
           +FGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD
Sbjct: 301 DFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMD 360

Query: 361 TGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG 420
           TGTAVTRLPTAAYVAFRDSFTA TSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG
Sbjct: 361 TGTAVTRLPTAAYVAFRDSFTALTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDG 420

Query: 421 PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 480
           PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC
Sbjct: 421 PVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 480

BLAST of CmoCh18G008170 vs. ExPASy TrEMBL
Match: A0A1S3BEG7 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103488783 PE=4 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 1.2e-224
Identity = 386/476 (81.09%), Postives = 427/476 (89.71%), Query Frame = 0

Query: 8   SLLLFFSVSVVFGSR---SSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKSN 67
           SLL F +V VV  +    SSP +FQYLNVK++K D ND QILH LNFS+ HR V+G KS+
Sbjct: 9   SLLFFLAVLVVVAAAARPSSPTKFQYLNVKATKLDFNDGQILHTLNFSDAHRQVSGHKSH 68

Query: 68  HTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQDNNYKVA 127
           +  FKL L+HRD+L H+HG  +GF++R+KRD  RVATLVRRLS     ++ V+D+ +KVA
Sbjct: 69  NDTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSH---GAAAVEDSRFKVA 128

Query: 128 NFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFN 187
           NF T VISGMEEG+GEYFVRIGVGSPPRSQYMVIDSGSDIVWVQC+PC +CYQQSDPVF+
Sbjct: 129 NFATDVISGMEEGSGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCKPCSRCYQQSDPVFD 188

Query: 188 PAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIGEVMIHD 247
           PA+S+S+ GVSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT+G+VMI D
Sbjct: 189 PADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLTVGQVMIRD 248

Query: 248 MAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTLEFGR 307
           +AIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG GSTGTLEFGR
Sbjct: 249 VAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGSTGTLEFGR 308

Query: 308 GAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVVMDTGTA 367
           GA+PVGATWISL+RNP APSFYYIGLAG+GVGG++V IPEETFQLTE+GTNGVVMDTGTA
Sbjct: 309 GALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSIPEETFQLTEFGTNGVVMDTGTA 368

Query: 368 VTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLT 427
           VTRLPT+AYVA RDSFTAQTSNLPRAPGVSIFDTCY+LNGFESVRVPTVSFYFSDGP LT
Sbjct: 369 VTRLPTSAYVALRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFYFSDGPALT 428

Query: 428 LPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
           LPA NFLIPVD  GTFC AFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFGPNIC
Sbjct: 429 LPAKNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFGPNIC 481

BLAST of CmoCh18G008170 vs. ExPASy TrEMBL
Match: A0A0A0KPR4 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G579060 PE=4 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.3e-223
Identity = 388/484 (80.17%), Postives = 427/484 (88.22%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSR----SSPVEFQYLNVKSSKFDANDSQILHILNFSNGHR 60
           M  K  FS LLF  V +V  +     SS  +FQYLNVK++K D ND QILH LNFS+GHR
Sbjct: 1   MFFKQLFSSLLFLLVVIVGAAAAARLSSSTKFQYLNVKATKLDFNDGQILHALNFSDGHR 60

Query: 61  LVTGRKSNHTKFKLKLVHRDELPHIHGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGV 120
            V+G KS++  FKL L+HRD+L H+HG  +GF++R+KRD  RVATLVRRLS      + V
Sbjct: 61  QVSGYKSDNNTFKLNLLHRDKLSHVHGHRRGFNDRMKRDAIRVATLVRRLSH--GAPAAV 120

Query: 121 QDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCY 180
           +D+ YKVANF T VISGME G+GEYFVRIGVGSPPR+QYMVIDSGSDIVWVQC+PC +CY
Sbjct: 121 KDSRYKVANFATDVISGMEAGSGEYFVRIGVGSPPRNQYMVIDSGSDIVWVQCKPCSRCY 180

Query: 181 QQSDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLT 240
           QQSDPVF+PA+S+S+ GVSCGS VCDRLEN GC+AGRCRYEVSYGDGSYTKGTLALETLT
Sbjct: 181 QQSDPVFDPADSSSFAGVSCGSDVCDRLENTGCNAGRCRYEVSYGDGSYTKGTLALETLT 240

Query: 241 IGEVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGS 300
           +G+VMI D+AIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSRG GS
Sbjct: 241 VGQVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRGTGS 300

Query: 301 TGTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNG 360
           TG LEFGRGA+PVGATWISL+RNP APSFYYIGLAG+GVGG++V +PEETFQLTEYGTNG
Sbjct: 301 TGALEFGRGALPVGATWISLIRNPRAPSFYYIGLAGIGVGGVRVSVPEETFQLTEYGTNG 360

Query: 361 VVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFY 420
           VVMDTGTAVTR PTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCY+LNGFESVRVPTVSFY
Sbjct: 361 VVMDTGTAVTRFPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYDLNGFESVRVPTVSFY 420

Query: 421 FSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFG 480
           FSDGPVLTLPA NFLIPVD  GTFC AFAPSPSGLSIIGNIQQ GIQISFDGANGFVGFG
Sbjct: 421 FSDGPVLTLPARNFLIPVDGGGTFCLAFAPSPSGLSIIGNIQQEGIQISFDGANGFVGFG 480

BLAST of CmoCh18G008170 vs. ExPASy TrEMBL
Match: A0A6J1KA06 (protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LOC111493608 PE=4 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 5.4e-217
Identity = 378/482 (78.42%), Postives = 419/482 (86.93%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60
           +LL LH  LL   + +      S P  F YLNVK +K + N +QIL  LNFS+ H  VT 
Sbjct: 10  LLLLLH--LLFLAAAAAALARPSLPPNFLYLNVKPTKLELNHTQILPTLNFSDAHLQVTA 69

Query: 61  RKSNHTKFKLKLVHRDELPHIHG--QHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120
           +K ++T  KL L+HRD+LPH+H       F+ERIKRD  RVATLVRRLS     ++ VQD
Sbjct: 70  QKFHNTTLKLNLLHRDKLPHVHAHRHRHAFNERIKRDAIRVATLVRRLSH---TAAAVQD 129

Query: 121 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180
           N+YKVANF TGV+SGMEEG+GE+FVR+GVGSPPRSQYMVIDSGSDIVWVQCQPC QCYQQ
Sbjct: 130 NSYKVANFATGVVSGMEEGSGEFFVRVGVGSPPRSQYMVIDSGSDIVWVQCQPCTQCYQQ 189

Query: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVFNPA+S+S+ GVSCGSAVC RL+N  CHAGRCRYEVSYGDGSYTKGTLALET+T+G
Sbjct: 190 SDPVFNPADSSSFAGVSCGSAVCGRLDNTDCHAGRCRYEVSYGDGSYTKGTLALETITMG 249

Query: 241 EVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300
           +VMI D+AIGCGHTNQGMFIGAAGLLGLGGGSMSF+GQLGGQTGGAFSYCLVSR  GSTG
Sbjct: 250 QVMIRDVAIGCGHTNQGMFIGAAGLLGLGGGSMSFIGQLGGQTGGAFSYCLVSRDTGSTG 309

Query: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360
           TLEFGRGAMPVGATWISL+RNP APSFYYIGLAGVGVGG++VPIPEETFQL+EYGTNGVV
Sbjct: 310 TLEFGRGAMPVGATWISLIRNPRAPSFYYIGLAGVGVGGVRVPIPEETFQLSEYGTNGVV 369

Query: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAY AFRD+FTAQT+NLPRA GVSIFDTC++LNGFES+RVPTVSFYFS
Sbjct: 370 MDTGTAVTRLPTAAYEAFRDAFTAQTNNLPRAGGVSIFDTCFDLNGFESIRVPTVSFYFS 429

Query: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480
           DGPVLTLPA NFLIPV+ AGTFC AFAPSPSGLSIIGNIQQ GIQIS DGANGFVGFGPN
Sbjct: 430 DGPVLTLPAKNFLIPVNGAGTFCLAFAPSPSGLSIIGNIQQEGIQISIDGANGFVGFGPN 486

BLAST of CmoCh18G008170 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 625.9 bits (1613), Expect = 2.6e-179
Identity = 310/482 (64.32%), Postives = 380/482 (78.84%), Query Frame = 0

Query: 1   MLLKLHFSLLLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTG 60
           MLL L F   L   + +   S  S  +FQ ++V         +    + +F+N H     
Sbjct: 1   MLLPLFF-FFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTAT----LPDFNNTH----F 60

Query: 61  RKSNHTKFKLKLVHRDELPHI--HGQHQGFDERIKRDVKRVATLVRRLSRYDTDSSGVQD 120
              + +K+ L+L+HRD  P +     H     R++RD  RV+ ++RR+S     SS   D
Sbjct: 61  SDESSSKYTLRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSS---D 120

Query: 121 NNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQ 180
           + Y+V +FG+ ++SGM++G+GEYFVRIGVGSPPR QYMVIDSGSD+VWVQCQPC+ CY+Q
Sbjct: 121 SRYEVNDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQ 180

Query: 181 SDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLALETLTIG 240
           SDPVF+PA+S SYTGVSCGS+VCDR+EN+GCH+G CRYEV YGDGSYTKGTLALETLT  
Sbjct: 181 SDPVFDPAKSGSYTGVSCGSSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFA 240

Query: 241 EVMIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGSTG 300
           + ++ ++A+GCGH N+GMFIGAAGLLG+GGGSMSFVGQL GQTGGAF YCLVSRG  STG
Sbjct: 241 KTVVRNVAMGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDSTG 300

Query: 301 TLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLTEYGTNGVV 360
           +L FGR A+PVGA+W+ LVRNP APSFYY+GL G+GVGG+++P+P+  F LTE G  GVV
Sbjct: 301 SLVFGREALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVV 360

Query: 361 MDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFS 420
           MDTGTAVTRLPTAAYVAFRD F +QT+NLPRA GVSIFDTCY+L+GF SVRVPTVSFYF+
Sbjct: 361 MDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFT 420

Query: 421 DGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPN 480
           +GPVLTLPA NFL+PVD +GT+CFAFA SP+GLSIIGNIQQ GIQ+SFDGANGFVGFGPN
Sbjct: 421 EGPVLTLPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPN 470

BLAST of CmoCh18G008170 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 389.0 bits (998), Expect = 5.4e-108
Identity = 201/452 (44.47%), Postives = 283/452 (62.61%), Query Frame = 0

Query: 41  NDSQILHILNFSNGHRLVTGRKSNH---TKFKLKLVHRDELPHI-HGQHQGFD-ERIKRD 100
           N +  +H   +++  RL    +  H   + F L+L  R  +    H  ++     R+ RD
Sbjct: 37  NVADSIHRTKYTSSFRLNQQEEQTHSASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRD 96

Query: 101 VKRVATLVRRL-------SRYDTDSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVG 160
             RV +L+ RL       S+ D           +  +    +ISG  +G+GEYF R+G+G
Sbjct: 97  TARVKSLITRLDLAINNISKADLKPISTMYTT-EEQDIEAPLISGTTQGSGEYFTRVGIG 156

Query: 161 SPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENAG 220
            P R  YMV+D+GSD+ W+QC PC  CY Q++P+F P+ S+SY  +SC +  C+ LE + 
Sbjct: 157 KPAREVYMVLDTGSDVNWLQCTPCADCYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSE 216

Query: 221 CHAGRCRYEVSYGDGSYTKGTLALETLTIGEVMIHDMAIGCGHTNQGMFIGAAGLLGLGG 280
           C    C YEVSYGDGSYT G  A ETLTIG  ++ ++A+GCGH+N+G+F+GAAGLLGLGG
Sbjct: 217 CRNATCLYEVSYGDGSYTVGDFATETLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGG 276

Query: 281 GSMSFVGQLGGQTGGAFSYCLVSRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFYYI 340
           G ++   QL      +FSYCLV R   S  T++FG    P  A    L+RN    +FYY+
Sbjct: 277 GLLALPSQLNTT---SFSYCLVDRDSDSASTVDFGTSLSP-DAVVAPLLRNHQLDTFYYL 336

Query: 341 GLAGVGVGGIKVPIPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLP 400
           GL G+ VGG  + IP+ +F++ E G+ G+++D+GTAVTRL T  Y + RDSF   T +L 
Sbjct: 337 GLTGISVGGELLQIPQSSFEMDESGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLE 396

Query: 401 RAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFAPSP 460
           +A GV++FDTCYNL+   +V VPTV+F+F  G +L LPA N++IPVDS GTFC AFAP+ 
Sbjct: 397 KAAGVAMFDTCYNLSAKTTVEVPTVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTA 456

Query: 461 SGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
           S L+IIGN+QQ G +++FD AN  +GF  N C
Sbjct: 457 SSLAIIGNVQQQGTRVTFDLANSLIGFSSNKC 483

BLAST of CmoCh18G008170 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 384.8 bits (987), Expect = 1.0e-106
Identity = 194/431 (45.01%), Postives = 277/431 (64.27%), Query Frame = 0

Query: 63  SNHTKFKLKLVHRDELPHIHGQHQGFD----ERIKRDVKRVATLVRRL-------SRYDT 122
           ++ +   L+L  RD    +  QH+ +      R++RD  RVA +V ++        R D 
Sbjct: 75  NSSSPLSLELHSRDTF--VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDL 134

Query: 123 DSSGVQDNNYKVANFGTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQP 182
                +D  Y+  +  T V+SG  +G+GEYF RIGVG+P +  Y+V+D+GSD+ W+QC+P
Sbjct: 135 KPVYNEDTRYQTEDLTTPVVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEP 194

Query: 183 CRQCYQQSDPVFNPAESTSYTGVSCGSAVCDRLENAGCHAGRCRYEVSYGDGSYTKGTLA 242
           C  CYQQSDPVFNP  S++Y  ++C +  C  LE + C + +C Y+VSYGDGS+T G LA
Sbjct: 195 CADCYQQSDPVFNPTSSSTYKSLTCSAPQCSLLETSACRSNKCLYQVSYGDGSFTVGELA 254

Query: 243 LETLTIGEV-MIHDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLV 302
            +T+T G    I+++A+GCGH N+G+F GAAGLLGLGGG +S   Q+      +FSYCLV
Sbjct: 255 TDTVTFGNSGKINNVALGCGHDNEGLFTGAAGLLGLGGGVLSITNQM---KATSFSYCLV 314

Query: 303 SRGIGSTGTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVPIPEETFQLT 362
            R  G + +L+F    +  G     L+RN    +FYY+GL+G  VGG KV +P+  F + 
Sbjct: 315 DRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVD 374

Query: 363 EYGTNGVVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPR-APGVSIFDTCYNLNGFESVR 422
             G+ GV++D GTAVTRL T AY + RD+F   T NL + +  +S+FDTCY+ +   +V+
Sbjct: 375 ASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVK 434

Query: 423 VPTVSFYFSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGA 481
           VPTV+F+F+ G  L LPA N+LIPVD +GTFCFAFAP+ S LSIIGN+QQ G +I++D +
Sbjct: 435 VPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLS 494

BLAST of CmoCh18G008170 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 378.3 bits (970), Expect = 9.5e-105
Identity = 206/465 (44.30%), Postives = 284/465 (61.08%), Query Frame = 0

Query: 23  SSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKSNHTKFKLKLVHRDELPHIH 82
           +SPV FQ           +DS+ L    F +G         + +   L L H D L    
Sbjct: 41  ASPVSFQ---------PDSDSESLLESEFESG-----SDSESSSSITLNLDHIDALSSNK 100

Query: 83  GQHQGFDERIKRD---VKRVATLVRRLSRYDTDSSGVQDNNYKVANFGTGVISGMEEGNG 142
              + F  R++RD   VK +ATL  ++   +   +       +   F + V+SG+ +G+G
Sbjct: 101 TPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAP------RPGGFSSSVVSGLSQGSG 160

Query: 143 EYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFNPAESTSYTGVSCGSA 202
           EYF R+GVG+P R  YMV+D+GSDIVW+QC PCR+CY QSDP+F+P +S +Y  + C S 
Sbjct: 161 EYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSP 220

Query: 203 VCDRLENAGCHAGR--CRYEVSYGDGSYTKGTLALETLTIGEVMIHDMAIGCGHTNQGMF 262
            C RL++AGC+  R  C Y+VSYGDGS+T G  + ETLT     +  +A+GCGH N+G+F
Sbjct: 221 HCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEGLF 280

Query: 263 IGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSRGIGS-TGTLEFGRGAMPVGATWISL 322
           +GAAGLLGLG G +SF GQ G +    FSYCLV R   S   ++ FG  A+   A +  L
Sbjct: 281 VGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFTPL 340

Query: 323 VRNPNAPSFYYIGLAGVGVGGIKVP-IPEETFQLTEYGTNGVVMDTGTAVTRLPTAAYVA 382
           + NP   +FYY+GL G+ VGG +VP +    F+L + G  GV++D+GT+VTRL   AY+A
Sbjct: 341 LSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIA 400

Query: 383 FRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFYFSDGPVLTLPANNFLIPVD 442
            RD+F      L RAP  S+FDTC++L+    V+VPTV  +F  G  ++LPA N+LIPVD
Sbjct: 401 MRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVD 460

Query: 443 SAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFGPNIC 481
           + G FCFAFA +  GLSIIGNIQQ G ++ +D A+  VGF P  C
Sbjct: 461 TNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of CmoCh18G008170 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 357.1 bits (915), Expect = 2.3e-98
Identity = 202/484 (41.74%), Postives = 290/484 (59.92%), Query Frame = 0

Query: 10  LLFFSVSVVFGSRSSPVEFQYLNVKSSKFDANDSQILHILNFSNGHRLVTGRKS-NHTKF 69
           L F   +V+F + S+  ++Q L V +    A        L++     L     S + T  
Sbjct: 9   LAFSVFAVLFFTSSASSQYQTLVVNTLPSSAT-------LSWPESESLTDESLSESTTSL 68

Query: 70  KLKLVHRDELPHIHGQHQG--FDERIKRDVKRVATLVRRLSRYDTDSSGVQDNNYKVANF 129
            + L H D L           F+ R++RD  RV + +  L+   T  +  +        F
Sbjct: 69  SVHLSHVDALSSFSDASPADLFNLRLQRDSLRVKS-ITSLAAVSTGRNATKRTPRTAGGF 128

Query: 130 GTGVISGMEEGNGEYFVRIGVGSPPRSQYMVIDSGSDIVWVQCQPCRQCYQQSDPVFNPA 189
              VISG+ +G+GEYF+R+GVG+P  + YMV+D+GSD+VW+QC PC+ CY Q+D +F+P 
Sbjct: 129 SGAVISGLSQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPK 188

Query: 190 ESTSYTGVSCGSAVCDRLENAG-CHAGR---CRYEVSYGDGSYTKGTLALETLTIGEVMI 249
           +S ++  V CGS +C RL+++  C   R   C Y+VSYGDGS+T+G  + ETLT     +
Sbjct: 189 KSKTFATVPCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARV 248

Query: 250 HDMAIGCGHTNQGMFIGAAGLLGLGGGSMSFVGQLGGQTGGAFSYCLVSR-GIGST---- 309
             + +GCGH N+G+F+GAAGLLGLG G +SF  Q   +  G FSYCLV R   GS+    
Sbjct: 249 DHVPLGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPP 308

Query: 310 GTLEFGRGAMPVGATWISLVRNPNAPSFYYIGLAGVGVGGIKVP-IPEETFQLTEYGTNG 369
            T+ FG  A+P  + +  L+ NP   +FYY+ L G+ VGG +VP + E  F+L   G  G
Sbjct: 309 STIVFGNAAVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGG 368

Query: 370 VVMDTGTAVTRLPTAAYVAFRDSFTAQTSNLPRAPGVSIFDTCYNLNGFESVRVPTVSFY 429
           V++D+GT+VTRL   AYVA RD+F    + L RAP  S+FDTC++L+G  +V+VPTV F+
Sbjct: 369 VIIDSGTSVTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFH 428

Query: 430 FSDGPVLTLPANNFLIPVDSAGTFCFAFAPSPSGLSIIGNIQQGGIQISFDGANGFVGFG 481
           F  G V +LPA+N+LIPV++ G FCFAFA +   LSIIGNIQQ G ++++D     VGF 
Sbjct: 429 FGGGEV-SLPASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFL 483

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHE33.7e-17864.32Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LS401.4e-10545.01Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
Q9LNJ31.3e-10344.30Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q8S9J64.6e-7237.50Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LEW38.2e-6935.18Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G1D02.2e-279100.00protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1HQG02.0e-26795.63protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A1S3BEG71.2e-22481.09protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A0A0KPR41.3e-22380.17Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G57906... [more]
A0A6J1KA065.4e-21778.42protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
AT3G20015.12.6e-17964.32Eukaryotic aspartyl protease family protein [more]
AT1G25510.15.4e-10844.47Eukaryotic aspartyl protease family protein [more]
AT3G18490.11.0e-10645.01Eukaryotic aspartyl protease family protein [more]
AT1G01300.19.5e-10544.30Eukaryotic aspartyl protease family protein [more]
AT3G61820.12.3e-9841.74Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 141..303
e-value: 5.6E-49
score: 166.8
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 325..476
e-value: 2.6E-31
score: 108.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 121..303
e-value: 8.5E-50
score: 171.4
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 310..480
e-value: 1.1E-43
score: 151.0
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 137..480
NoneNo IPR availablePANTHERPTHR13683:SF838PROTEIN ASPARTIC PROTEASE IN GUARD CELL 2-LIKEcoord: 64..480
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 64..480
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 141..476
score: 45.173145
IPR033873CND41-likeCDDcd05472cnd41_likecoord: 140..480
e-value: 4.26678E-129
score: 374.686

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh18G008170.1CmoCh18G008170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity